ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII3310  suspect: Pn   S     KOG2722  Function unknown                                               Predicted membrane protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII3310 1166092 1164482 -537 
         (537 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g76530 [S] KOG2722 Predicted membrane protein                       55   3e-07
SPAC5D6.04 [S] KOG2722 Predicted membrane protein                      51   4e-06
At1g76520 [S] KOG2722 Predicted membrane protein                       51   4e-06

>At1g76530 [S] KOG2722 Predicted membrane protein
          Length = 415

 Score = 55.1 bits (131), Expect = 3e-07
 Identities = 50/216 (23%), Positives = 97/216 (44%), Gaps = 13/216 (6%)

Query: 7   EAIWASVKPIIKIYMIIATGFGLAKG--NILTVAATRTCSDIVLTVLLPSLSFNKIVSSI 64
           E   AS KP+++  +I + GF LA    N+L   A +  ++IV  V  PSL  +++  S+
Sbjct: 5   ELFIASSKPVVETLLITSVGFYLALDTVNLLGHDARKHLNNIVFYVFSPSLIGSRLADSV 64

Query: 65  DYRDAKDVGIICLTAVMIFGTGLFFAFVVRKVLPVPKKWRGGILAGGTFPNISDLPIAIL 124
            Y     +  + +  ++ F  G    ++V  +   P + RG I++     N+  +P+ I+
Sbjct: 65  TYESLVKMWFMPVNVLLTFMIGSLLGWIVIVITKPPSQLRGLIISCCASGNLGTMPLIII 124

Query: 125 QTM--DQGLLFTEEEADK--GIASVIIFLAMFMICIFN------LGGFRLIEMDFEYADE 174
             +  ++G  F + E+ +  G+  V + +  F I ++       + G   + MD  Y + 
Sbjct: 125 PAICKEKGGPFGDSESCEKYGMGYVTLSMTAFFISVYKHDTNWYVSGGNGLLMDL-YINL 183

Query: 175 ENASIQTPLEPPAQSNASQFHLQTDSQSFCSKENDR 210
                 +P+E    S  S +      Q   SKE ++
Sbjct: 184 MRVLSNSPVETHTHSIESNYDDSCKVQLISSKEEEK 219


>SPAC5D6.04 [S] KOG2722 Predicted membrane protein
          Length = 452

 Score = 51.2 bits (121), Expect = 4e-06
 Identities = 53/229 (23%), Positives = 99/229 (43%), Gaps = 23/229 (10%)

Query: 9   IWASVKPIIK----IYMIIATGFGLAKGNILTVAATRTCSDIVLTVLLPSLSFNKIVSSI 64
           +W+ ++PII+    + +I   G+ LAK   L   A +  S + +    P L F K+ + +
Sbjct: 12  VWSLLRPIIESDLEVIVIALGGYVLAKKGFLPRDAQKVISSLNVYFFTPCLVFEKVGNGL 71

Query: 65  DYRDAKDVGIICLTAVMIFGTGLFFAFVVRKVLPVPKKWRGGILAGGTFPNISDLPIAI- 123
           + +   D+ ++ +  V+I    +  +F++ K+  +  + R    A  TF N + LP+A+ 
Sbjct: 72  NLKMLIDLSLLPVFYVIISAASILISFLLAKLFRLTPRQRNFATACITFQNSNSLPLALV 131

Query: 124 --LQTMDQGLLF---TEEEADKGIASVIIFLAMFMICIFNLGGFRLIEMDFEYADEENAS 178
             L T  + LL+    ++  DK  +  I++L +F      LG      + + Y      S
Sbjct: 132 SSLATTVKDLLWDKIPDDTPDKVASRGIMYLLIFS----QLGQ----ALRWSYGYRILLS 183

Query: 179 IQTPLEPPAQSNASQFHLQTDSQSF-----CSKENDRAVNSINSQEAVT 222
              P +P    N S  H   + +        S   D   NS+ + E  T
Sbjct: 184 PNQPEDPLPIGNRSWSHSDVNEEEIQNLLASSANVDGVQNSVQANEGST 232


>At1g76520 [S] KOG2722 Predicted membrane protein
          Length = 390

 Score = 51.2 bits (121), Expect = 4e-06
 Identities = 47/194 (24%), Positives = 92/194 (47%), Gaps = 15/194 (7%)

Query: 7   EAIWASVKPIIKIYMIIATGFGLAKG--NILTVAATRTCSDIVLTVLLPSLSFNKIVSSI 64
           E    S KP+++I +I + GF +A    N+L   A +  ++IV  V  PSL  +++  S+
Sbjct: 6   ELFITSSKPVVEILLITSVGFYMALDGVNLLGHDARKYLNNIVFYVFSPSLIGSRLADSV 65

Query: 65  DYRDAKDVGIICLTAVMIFGTGLFFAFVVRKVLPVPKKWRGGILAGGTFPNISDLPIAIL 124
            Y     +  + +  ++ F  G    ++V  +   P   RG IL      N+ ++P+ I+
Sbjct: 66  TYESLVKMWFMPVNVLLTFIIGSLLGWIVIVITKPPSHLRGLILGCCAAGNLGNMPLIII 125

Query: 125 QTM--DQGLLFTEEEADK--GIASVIIFLAMFMICIFNLGGFRLIEMDFEYADEENASIQ 180
             +  ++G  F + E+ +  G+  V + +AM  I I+    + L+ +        N+ ++
Sbjct: 126 PAVCKEKGGPFGDPESCQKYGMGYVALSMAMGSIYIWTY-VYNLMRV------LSNSPVE 178

Query: 181 TPLEPPAQSNASQF 194
           TP  P  +SN   +
Sbjct: 179 TP--PSVESNYDSY 190


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.325    0.141    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,748,991
Number of Sequences: 60738
Number of extensions: 1223012
Number of successful extensions: 3712
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3710
Number of HSP's gapped (non-prelim): 3
length of query: 537
length of database: 30,389,216
effective HSP length: 111
effective length of query: 426
effective length of database: 23,647,298
effective search space: 10073748948
effective search space used: 10073748948
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)