ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3336 good G KOG2798 Carbohydrate transport and metabolism Putative trehalase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3336 1174325 1175509 395
(395 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL092w [G] KOG2798 Putative trehalase 308 1e-83
SPBC1778.07 [G] KOG2798 Putative trehalase 186 4e-47
Hs20540987 [G] KOG2798 Putative trehalase 162 1e-39
At2g32160 [G] KOG2798 Putative trehalase 140 3e-33
7290235 [G] KOG2798 Putative trehalase 133 5e-31
At2g32170 [G] KOG2798 Putative trehalase 132 1e-30
CE16654 [G] KOG2798 Putative trehalase 129 8e-30
>YNL092w [G] KOG2798 Putative trehalase
Length = 400
Score = 308 bits (788), Expect = 1e-83
Identities = 161/392 (41%), Positives = 232/392 (59%), Gaps = 8/392 (2%)
Query: 5 DEENDVLLLERTASSFFRYKEFALEEFWRPRILRWESLSEHQKQMIPWYNSYLEDVHDAI 64
D + + + R SF +Y+E+AL+E + R+ +W S+S+ QK M+P Y YL ++ AI
Sbjct: 7 DNQRENKAVARVIISFLKYEEYALKEIYNLRVKKWASISDRQKDMVPNYTKYLANLKAAI 66
Query: 65 ETNSIFYMNLLERSVKLWGLNSDPNLWAQPSGTDMRKTISMLNQIAREWSTHCEEERDQX 124
N F+ ++ E +++ ++ +P QP+ DM KT S+L Q+ REWS ER+
Sbjct: 67 IENGKFFRSVAEYALQ--SISFEPGEIVQPNDLDMSKTCSLLTQVYREWSAEAISERNCL 124
Query: 125 XXXXXXXXXXXXPSDRNTIKILIPGAGLGRLAVEMVRLGFNTEANEVSYHMLMNSQFIMD 184
P + ILIPG G GRL V++ R+G+N E NE SYHML+ SQ++++
Sbjct: 125 NSRLVPFLKTLSPPKAD---ILIPGCGTGRLLVDLSRMGYNCEGNEFSYHMLLVSQYMLN 181
Query: 185 GGLQKEQIALFPFVHSFSHHINRAEQLRQVNIPDMNIVEEVGGNGLLSMVAGSFPDLYGP 244
GL + QI ++PF+H FSH +QL + +PD+ G G +S+ AGSF D YG
Sbjct: 182 AGLLQNQIIIYPFIHCFSHWKKIEDQLSPIKVPDIEAWSSNKGMGSMSICAGSFVDCYGR 241
Query: 245 NVNIKQSESYSNSAYIREVRAANRGSKHVVITNFFIDTCSNILDYLETITHVLKVDGYWI 304
N K S Y+ S ++ RA SK VV+TNFFIDT SNILDYL+TI HVLK G W
Sbjct: 242 NQGTKISSHYTFSRRMQLSRAKAENSKDVVVTNFFIDTGSNILDYLDTIGHVLKPGGIWC 301
Query: 305 NFGPFMYHFEQDHQTEMTADFDAYTGELSNILD-TPLRGIELSHEDILEVATTHFPFKLL 363
NFGP +YHFE DH E T + + Y+G I D TPL G+ELS +DI+ +AT H F+L+
Sbjct: 302 NFGPLLYHFENDHGVETTYEVNPYSGFQDKINDYTPLMGLELSSDDIISIATNHLDFELI 361
Query: 364 KQQTNISSSYG--TNKLDISMLGYQCSFWVLQ 393
++++ I YG +M GY C +W+L+
Sbjct: 362 RRESGILCGYGRYAGPESCAMPGYMCHYWILK 393
>SPBC1778.07 [G] KOG2798 Putative trehalase
Length = 373
Score = 186 bits (473), Expect = 4e-47
Identities = 127/399 (31%), Positives = 200/399 (49%), Gaps = 43/399 (10%)
Query: 6 EENDVLLLERTASSFFRYKEFALEEFWRPRILRWESLSEHQKQMIPWYNS----YLEDVH 61
+E +V +L+ S+FF Y+++A + R EH+ ++ ++ +L +
Sbjct: 4 DEEEVKVLKEVLSAFFLYRQYAHTITQQKRKSMSRLSFEHKDLLLQDSDNNFLKHLSRID 63
Query: 62 DAIETNSIFYMNLLERSVKLWGLNSDPNLWAQPSGTDMRKTISMLNQIAREWSTHCEEER 121
IE NS+ + ++ ++ + D N + M+K S L QIAR+WST C EER
Sbjct: 64 QCIEQNSVLAEAIANAAIPVFCSDFDQNELFHVNVDMMQKVSSTLKQIARDWSTECVEER 123
Query: 122 DQXXXXXXXXXXXXXPSD---RNTIKILIPGAGLGRLAVEMVRLGFNTEANEVSYHMLMN 178
PSD R+ I++L+PG+GLGRLA ++ GF + NE SY ML+
Sbjct: 124 RTTYAPFIEELNSLFPSDSIDRSKIRVLVPGSGLGRLAFDIAVEGFACQGNEFSYFMLLT 183
Query: 179 SQFIMDGGLQKEQIALFPFVHSFSHHINRAEQLRQVNIPDMNIVEEVGGNGLLSMVAGSF 238
S FI++ Q+ Q ++P++HSFS+H+ R +Q+R +NIPD + + + SM AG F
Sbjct: 184 SHFILNCVKQENQFLVYPYIHSFSNHVMRDDQVRSLNIPDAVPSQYLRNSQNFSMAAGDF 243
Query: 239 PDLYGPNVNIKQSESYSNSAYIREVRAANRGSKHVVITNFFIDTCSNILDYLETITHVLK 298
++YG +R S VV T FFIDT NILDYL+TI + L
Sbjct: 244 LEVYGTE--------------------ESRDSFQVVATCFFIDTTKNILDYLDTIKNCLV 283
Query: 299 VDGYWINFGPFMYHFEQDHQTEMTADFDAYTGELSNILDTPLRGIELSHEDILEVATTHF 358
GYWIN GP +YHFE + + +D P +EL+ E + V +
Sbjct: 284 DGGYWINLGPLLYHFESEGTSNSNSDSQ----------QQPF--VELTLEQLFYVMDS-M 330
Query: 359 PFKLLKQQTNISSSYGTNKLDISMLGYQCSFWV--LQKT 395
F++LK ++ ++Y +K + Y +WV LQK+
Sbjct: 331 GFEVLK-HNSVDTTYMGDKRSMLEWIYHPHYWVCRLQKS 368
>Hs20540987 [G] KOG2798 Putative trehalase
Length = 475
Score = 162 bits (409), Expect = 1e-39
Identities = 111/381 (29%), Positives = 184/381 (48%), Gaps = 53/381 (13%)
Query: 21 FRYKEFALEEFWRPRILRWESLSEHQKQMIPWYNSYLEDVHDAIETNSIFYMNLLERSVK 80
FRY ++ E ++ SL +Q++++P + +L+ + I+ N + ++ +
Sbjct: 139 FRYYGTSMHERVNRTERQFRSLPANQQKLLPQFLLHLDKIRKCIDHNQEILLTIVNDCIH 198
Query: 81 LWG---LNSDPNLWAQPSGT-DMRKTISMLNQIAREWSTHCEEERDQXXXXXXXXXXXXX 136
++ D N P+ T DM K S L Q R+WS + ERD
Sbjct: 199 MFENKEYGEDGNGKIMPASTFDMDKLKSTLKQFVRDWSETGKAERDACYQPIIKEILKNF 258
Query: 137 PSDR---NTIKILIPGAGLGRLAVEMVRLGFNTEANEVSYHMLMNSQFIMDGGLQKEQIA 193
P +R + + IL+PGAGLGRLA E+ LG+ + NE S+ ML +S F+++ + +
Sbjct: 259 PKERWDPSKVNILVPGAGLGRLAWEIAMLGYACQGNEWSFFMLFSSNFVLNRCSEINKYK 318
Query: 194 LFPFVHSFSHHINRAEQLRQVNIPDMNIVEEVGGNGLLSMVAGSFPDLYGPNVNIKQSES 253
L+P++H FS++ A+Q+R + PD++ G+ SM AG F ++Y
Sbjct: 319 LYPWIHQFSNNRRSADQIRPIFFPDVDPHSLPPGSN-FSMTAGDFQEIY----------- 366
Query: 254 YSNSAYIREVRAANRGSKHVVITNFFIDTCSNILDYLETITHVLKVDGYWINFGPFMYHF 313
+ + + T FFIDT N++DY++TI +LK G WIN GP +YHF
Sbjct: 367 ------------SECNTWDCIATCFFIDTAHNVIDYIDTIWKILKPGGIWINLGPLLYHF 414
Query: 314 EQDHQTEMTADFDAYTGELSNILDTPLRGIELSHEDILEVATTHFPFKLLKQQTNISSSY 373
E L+N L IELS+EDI V + FK+ ++ ++ S+Y
Sbjct: 415 E----------------NLANEL-----SIELSYEDIKNV-VLQYGFKVEVEKESVLSTY 452
Query: 374 GTNKLDISMLGYQCSFWVLQK 394
N L + Y+C +V++K
Sbjct: 453 TVNDLSMMKYYYECVLFVVRK 473
>At2g32160 [G] KOG2798 Putative trehalase
Length = 424
Score = 140 bits (354), Expect = 3e-33
Identities = 95/311 (30%), Positives = 152/311 (48%), Gaps = 44/311 (14%)
Query: 87 DPNLWAQPSGTDMRKTISMLNQIAREWSTHCEEERDQXXXXXXXXXXXXXPS---DRNTI 143
D +L A D+ K ++ I R+W + ERD+ P +
Sbjct: 148 DSSLQAHVPLVDVNKVRWVIRNIVRDWGAEGQRERDECYKPILEELDSLFPDRHKESTRP 207
Query: 144 KILIPGAGLGRLAVEMVRLGFNTEANEVSYHMLMNSQFIMDGGLQKEQIALFPFVHSFSH 203
L+PGAGLGRLA+E+ LG ++ NEVSY+M++ S FI++ + ++P++H+ +
Sbjct: 208 ACLVPGAGLGRLALEISCLGMQSQGNEVSYYMMLCSSFILNYTQLPGEWTIYPWIHTNCN 267
Query: 204 HINRAEQLRQVNIPDMNIVEEVGGNGLLSMVAGSFPDLYGPNVNIKQSESYSNSAYIREV 263
++ +QLR ++IPD++ G SM G F +++
Sbjct: 268 SLSDDDQLRPISIPDIH-PASAGVTESFSMCRGDFVEVFN-------------------- 306
Query: 264 RAANRGSKHVVITNFFIDTCSNILDYLETITHVLKVDGYWINFGPFMYHFEQDHQTEMTA 323
++ G V+T FFIDT NI++Y+ETI+ +LK G IN GP +YHF A
Sbjct: 307 ESSQAGMWDAVVTCFFIDTAHNIIEYIETISKILKDGGVLINLGPLLYHF---------A 357
Query: 324 DFDAYTGELSNILDTPLRGIELSHEDILEVATTHFPFKLLKQQTNISSSYGTNKLDISML 383
D E+S IELS ED+ VA +H+ F++ K++T I ++Y TN +
Sbjct: 358 DEQGLENEMS---------IELSLEDVKRVA-SHYGFEMEKEKT-IETTYSTNPRSMMKN 406
Query: 384 GYQCSFWVLQK 394
Y FW ++K
Sbjct: 407 RYYPVFWTMRK 417
>7290235 [G] KOG2798 Putative trehalase
Length = 456
Score = 133 bits (334), Expect = 5e-31
Identities = 111/417 (26%), Positives = 178/417 (42%), Gaps = 75/417 (17%)
Query: 5 DEENDVLLLERTASSFFRYKEFALEEFWRPRILRWESLSEHQKQMIPWYNSYLEDVHDAI 64
+EE + +++ ++F Y +A + R SLS + M+ Y +LE V I
Sbjct: 26 NEEEEEKHIQKVQNAFLYYGPYACQRLKRSMDYL-NSLSGEDQIMLAKYRGHLECVRTCI 84
Query: 65 ETNSIFYMNLLERSVKLWGLNS---DPNLWAQPSGT---------------------DMR 100
+ N +L V L+ + DP+ + +P +
Sbjct: 85 DRNQAVIREILRGRV-LYPTDEATGDPSEFDEPPPNVRHGDMDQIDIPFDEADVEPLKIL 143
Query: 101 KTISMLNQIAREWSTHCEEERDQXXX---XXXXXXXXXXPSDRNTIKILIPGAGLGRLAV 157
K S L IAR+WST ER+Q + IKIL+PGAGLGRL
Sbjct: 144 KAQSTLKLIARDWSTEGALEREQSYKPIIDSIVAYFKHSDFELKDIKILVPGAGLGRLTY 203
Query: 158 EMVRLGFNTEANEVSYHMLMNSQFIMDGGLQKEQIALFPFVHSFSHHINRAEQLRQVNIP 217
E+ LG++ E NE SY ML+ S F+++ + + L+P+VH + +++ R +Q+ V P
Sbjct: 204 ELACLGYSCEGNEFSYFMLIASNFVLNLCDNENKYVLYPWVHQYVNNLRREDQVAPVRFP 263
Query: 218 DMNIVEEVGGNGLLSMVAGSFPDLYGPNVNIKQSESYSNSAYIREVRAANRGSKHVVITN 277
D+ ++ G + AG F ++Y K +Y + V T
Sbjct: 264 DVCPLKN-PPKGHFEIAAGDFLEVY------KTPNAY-----------------NCVATC 299
Query: 278 FFIDTCSNILDYLETITHVLKVDGYWINFGPFMYHFEQDHQTEMTADFDAYTGELSNILD 337
FFID +N++D++ TI +L G W+N GP +YHF +G+ S
Sbjct: 300 FFIDCANNVIDFIRTIYKILVPGGIWVNLGPLLYHFSD------------VSGQNS---- 343
Query: 338 TPLRGIELSHEDILEVATTHFPFKLLKQQTNISSSYGTNKLDISMLGYQCSFWVLQK 394
IE + ED+ + F + K +T I + Y N + YQ FWV +K
Sbjct: 344 -----IEPAFEDLC-IIMESVGFVIEKSRTGIRTKYAQNPSSMKQSEYQSLFWVCRK 394
>At2g32170 [G] KOG2798 Putative trehalase
Length = 306
Score = 132 bits (331), Expect = 1e-30
Identities = 87/255 (34%), Positives = 139/255 (54%), Gaps = 31/255 (12%)
Query: 146 LIPGAGLGRLAVEMVRLGFNTEANEVSYHMLMNSQFIMDGGLQKEQIALFPFVHSFSHHI 205
L+PGAGLGRLA+E+ LGF ++ NE SY+M++ S FI++ + ++P++HS + +
Sbjct: 70 LVPGAGLGRLALEISCLGFISQGNEFSYYMMICSSFILNYTQVPGEWTIYPWIHSNCNSL 129
Query: 206 NRAEQLRQVNIPDMNIVEEVGGN----GLLSM-VAGSFPDLYGPNVNIKQSESYSNSAYI 260
+ +QLR + IPD++ N GL + GSF + I + S ++
Sbjct: 130 SDNDQLRPIAIPDIHPARYALSNNSRTGLWFLECVGSF-----SSAGITEGFSMCGGDFV 184
Query: 261 REV-RAANRGSKHVVITNFFIDTCSNILDYLETITHVLKVDGYWINFGPFMYHFEQDHQT 319
+++ G V+T FFIDT N+++Y++TI+ +LK G WIN GP +YHF
Sbjct: 185 EVYNESSHAGMWDAVVTCFFIDTAHNVIEYIQTISKILKDGGVWINLGPLLYHF------ 238
Query: 320 EMTADFDAYTGELSNILDTPLRGIELSHEDILEVATTHFPFKLLKQQTNISSSYGTNKLD 379
AD + E+S IELS ED+ VA +HF F + K++T I ++Y TN
Sbjct: 239 ---ADTYGHENEMS---------IELSLEDVKRVA-SHFGFVIEKERT-IETTYTTNPRA 284
Query: 380 ISMLGYQCSFWVLQK 394
+ Y +FW ++K
Sbjct: 285 MMQNRYYTAFWTMRK 299
>CE16654 [G] KOG2798 Putative trehalase
Length = 369
Score = 129 bits (324), Expect = 8e-30
Identities = 100/396 (25%), Positives = 178/396 (44%), Gaps = 65/396 (16%)
Query: 13 LERTASSFFRYKEFALEEFWRPRILRWESLSEHQKQMIPWYNSYLEDVHDAIETNSIFYM 72
+E ++FF Y + E+ + ++HQ + + ++ ++ + +E NS
Sbjct: 19 MEEVINAFFYYGRYGAEKMVSMVEQMRKIPNDHQLLLGGQFKEHIRNMKEKVEHNSQILR 78
Query: 73 NLLERSVKLWGLNSDPNLWA-------QPSGTDMRKTISMLNQIAREWSTHCEEERDQXX 125
+ ++G + +L A +PS M K S + QI REWS+ + ER+
Sbjct: 79 MIANSCAGMFG---EDHLRAMRIHQTRRPSSDFMSKVFSTMRQICREWSSEGQPEREATF 135
Query: 126 XXXXXXXXXXXPSD---RNTIKILIPGAGLGRLAVEMVRLGFNTEANEVSYHMLMNSQFI 182
P + R+ ++IL+PG GLGRLA +++ GF + NE ++ ML S FI
Sbjct: 136 KPIIDQLTELYPPETHPRHNVRILVPGCGLGRLAYDLMEQGFTVQGNEFAFFMLFTSFFI 195
Query: 183 MDGGLQKEQIALFPFVHSFSHHINRAEQLRQVNIPDMNIVEEVGGN---GLLSMVAGSFP 239
++ Q+ Q ++PFV ++ N ++QLR V PD V + N S+ AG F
Sbjct: 196 INNCKQENQFTIYPFVFDKNNSWNYSDQLRPVTFPDKAPVSKKDPNHRRASFSICAGDFL 255
Query: 240 DLYGPNVNIKQSESYSNSAYIREVRAANRGSKHVVITNFFIDTCSNILDYLETITHVLKV 299
++ +K + + V++T +FIDT N+L+Y++ I L+
Sbjct: 256 EV------VKDT------------------TFDVIVTAWFIDTAHNVLEYIDAIYSTLEP 291
Query: 300 DGYWINFGPFMYHFEQDHQTEMTADFDAYTGELSNILDTP-LRGIELSHEDILEVATTHF 358
G WIN GP +HF DTP IEL + I+E+
Sbjct: 292 GGVWINVGPLTWHFS----------------------DTPDEASIELPYSVIMEMIRKK- 328
Query: 359 PFKLLKQQTNISSSYGTNKLDISMLGYQCSFWVLQK 394
F++++++ I S Y N+ + + C+++ +K
Sbjct: 329 GFQVVEERV-IDSKYTVNRRAMQFNQFACAYFYARK 363
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.135 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,188,850
Number of Sequences: 60738
Number of extensions: 949856
Number of successful extensions: 2216
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2188
Number of HSP's gapped (non-prelim): 9
length of query: 395
length of database: 30,389,216
effective HSP length: 108
effective length of query: 287
effective length of database: 23,829,512
effective search space: 6839069944
effective search space used: 6839069944
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)