ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3395 good R KOG1012 General function prediction only Ca2+-dependent lipid-binding protein CLB1/vesicle protein vp115/Granuphilin A, contains C2 domain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3395 1189822 1193340 1173
(1173 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR086c [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/ve... 1369 0.0
YNL087w [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/ve... 1251 0.0
YML072c [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/ve... 446 e-125
SPAPYUK71.03c [R] KOG1012 Ca2+-dependent lipid-binding protein C... 443 e-124
SPCC962.01 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1... 373 e-103
At1g20080 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 125 3e-28
At5g04220 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 108 5e-23
ECU01g1250 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1... 106 2e-22
7301181 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/ve... 101 6e-21
At5g11100 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 98 7e-20
SPCP31B10.06 [R] KOG1012 Ca2+-dependent lipid-binding protein CL... 96 4e-19
At1g05500 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 92 4e-18
Hs14149680 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1... 88 9e-17
CE18256 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/ve... 85 8e-16
At3g61050 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 82 6e-15
Hs21362014 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bin... 77 2e-13
At1g53590 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 75 6e-13
At2g20990 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 74 1e-12
Hs6005997 [U] KOG1013 Synaptic vesicle protein rabphilin-3A 72 7e-12
Hs18584396 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bin... 69 6e-11
CE28229 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein... 69 6e-11
CE02711 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein... 69 6e-11
Hs21361472 [O] KOG0940 Ubiquitin protein ligase RSP5/NEDD4 68 1e-10
7295906 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein... 67 1e-10
CE07234 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein... 67 2e-10
7302558 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bindin... 66 3e-10
Hs20482609 [TU] KOG1028 Ca2+-dependent phospholipid-binding prot... 66 4e-10
Hs14150054 [TU] KOG1028 Ca2+-dependent phospholipid-binding prot... 65 8e-10
Hs4506067 [T] KOG0696 Serine/threonine protein kinase 64 1e-09
Hs4507337 [TU] KOG1028 Ca2+-dependent phospholipid-binding prote... 64 2e-09
At1g50260 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 62 4e-09
7302860 [T] KOG0696 Serine/threonine protein kinase 62 4e-09
Hs22043941 [TU] KOG1028 Ca2+-dependent phospholipid-binding prot... 62 5e-09
7302861 [T] KOG0696 Serine/threonine protein kinase 62 5e-09
CE27898 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bindin... 62 7e-09
CE26302 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein... 62 7e-09
CE09100 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bindin... 62 7e-09
Hs11128045_3 [R] KOG1012 Ca2+-dependent lipid-binding protein CL... 61 9e-09
Hs8922917 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bind... 61 1e-08
CE02586 [T] KOG0696 Serine/threonine protein kinase 60 2e-08
At3g14590 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 60 2e-08
Hs20127450 [T] KOG0696 Serine/threonine protein kinase 59 3e-08
Hs13384594 [T] KOG0696 Serine/threonine protein kinase 59 3e-08
At1g63220 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bind... 59 4e-08
Hs5032139 [TU] KOG1028 Ca2+-dependent phospholipid-binding prote... 58 8e-08
At3g19830 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/... 58 8e-08
7304322 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein... 58 8e-08
Hs22068916 [T] KOG0696 Serine/threonine protein kinase 58 1e-07
CE24954 [U] KOG1013 Synaptic vesicle protein rabphilin-3A 58 1e-07
At1g03370_2 [S] KOG1032 Uncharacterized conserved protein contai... 57 1e-07
Hs22045984 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1... 57 2e-07
Hs15299160 [TU] KOG1028 Ca2+-dependent phospholipid-binding prot... 57 2e-07
Hs5901958 [T] KOG2059 Ras GTPase-activating protein 56 4e-07
Hs20550102 [O] KOG0940 Ubiquitin protein ligase RSP5/NEDD4 56 4e-07
7301258 [UT] KOG1328 Synaptic vesicle protein BAIAP3 involved in... 55 6e-07
At3g07940_2 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bi... 55 6e-07
Hs4503353 [U] KOG1013 Synaptic vesicle protein rabphilin-3A 55 8e-07
Hs20552506 [TU] KOG1028 Ca2+-dependent phospholipid-binding prot... 54 1e-06
Hs7662432 [U] KOG1013 Synaptic vesicle protein rabphilin-3A 54 2e-06
Hs4759026 [T] KOG2059 Ras GTPase-activating protein 53 2e-06
Hs14762054 [TU] KOG1028 Ca2+-dependent phospholipid-binding prot... 53 3e-06
Hs18543827 [TU] KOG1028 Ca2+-dependent phospholipid-binding prot... 52 4e-06
CE29448 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein... 52 4e-06
Hs4504797_2 [T] KOG4305 RhoGEF GTPase 52 7e-06
At4g05330_2 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bi... 52 7e-06
Hs4758856 [M] KOG1326 Membrane-associated protein FER-1 and rela... 51 9e-06
Hs20553661 [T] KOG1327 Copine 51 9e-06
At1g70800 [R] KOG1030 Predicted Ca2+-dependent phospholipid-bind... 51 9e-06
>YOR086c [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 1186
Score = 1369 bits (3543), Expect = 0.0
Identities = 664/1159 (57%), Positives = 866/1159 (74%), Gaps = 9/1159 (0%)
Query: 1 MASAGTDIVNPASGE--QVVQVDGVDKLAP--TAALSSDAGNVSEEKQVDPAKTSSEVRP 56
MA T + P E QV ++G+DKL P T + + +VS EK A S R
Sbjct: 1 MAKEDTGVTAPKKPETAQVANINGIDKLEPPKTKEETESSKSVSSEKAAH-ASDESFKRS 59
Query: 57 VIDATYVGWKQMGGWEESDTLTFEDELLGVNEETFLDNVIPDKAYGDWYHAVGIFFMXXX 116
+ +A+YVGWKQ+GGWE+ D LT +DEL+ + ETFLDN+IPD YGDWYH+V IFF+
Sbjct: 60 IHEASYVGWKQIGGWEDKDELTLDDELMDMTRETFLDNIIPDSLYGDWYHSVAIFFIGGV 119
Query: 117 XXXXXXXXXXXXXPVFFIILATAVFYRTSVKKYRMSIRELAQKEFVVQKVEDDYESMEWL 176
FF+I+ T++ YRTS KKYR SIREL QKEF VQKVE+DYES+EWL
Sbjct: 120 ASFALGHYKFSMGSAFFVIVITSLLYRTSAKKYRGSIRELVQKEFTVQKVENDYESLEWL 179
Query: 177 NSFLDKYWPIIEPSVSQIVVEQVNEQIAVNEAIPAFVKALWIDRFTLGIKPPRIDLVKTF 236
N+FLDKYWPI+EPSVSQ++V+Q NEQ+A NEAIP F+ LWID TLG+KPPR+DLVKTF
Sbjct: 180 NAFLDKYWPILEPSVSQLIVQQANEQMATNEAIPKFITQLWIDELTLGVKPPRVDLVKTF 239
Query: 237 QNTELDVVVMDFGMSFTPHDLSDLTSKQLRNYVNQTVVLKAKLFGLTVPVVVADIAFKAR 296
QNT DVVVMD+G+SFTPHDL D+++KQ+RNYVN+ V+KAK+FG+T+PV V+DIAFKA
Sbjct: 240 QNTASDVVVMDWGISFTPHDLCDMSAKQVRNYVNELAVVKAKIFGITIPVSVSDIAFKAH 299
Query: 297 VRVRMKLMTPFPHIETVNIQFLDVPDIDFVCKLLGNTVFNWEIMSIPGLLPLARELARKY 356
RV+ KLMTPFPH+ETVNIQ L VPD DFV L G ++FNWEI++IPGL+ L +++A+KY
Sbjct: 300 ARVKFKLMTPFPHVETVNIQLLKVPDFDFVATLFGRSIFNWEILAIPGLMTLIQKMAKKY 359
Query: 357 LGPLFLPPFSLQLNIPQLVSGSALSIGVLELTVKNAKDLKR-SNLMNISVDPYLQFSIGG 415
+GP+ LPPFSLQLNIPQL+SGS LSIG+LE+TVKNAK LKR S+++N S+DPYL F
Sbjct: 360 MGPILLPPFSLQLNIPQLLSGSNLSIGILEITVKNAKGLKRTSSILNESIDPYLSFEFND 419
Query: 416 RVLGKTRTVKDTLNPVWNESMFILLASFTDPLEITVYDKREHLKDKVLGRIYYNLSSLHD 475
+ KTRTV+DTLNPVW+E++++LL SFTDPL I+VYDKR LKDKVLGRI YNL++LHD
Sbjct: 420 ISIAKTRTVRDTLNPVWDETLYVLLNSFTDPLTISVYDKRAKLKDKVLGRIQYNLNTLHD 479
Query: 476 TPLQKNVSSHFIRNSKPVGDLFFDMKFHATLESKKLPDGTVEEVPDLNTGITKITIEEAK 535
Q+N+ + F+RNSKPVG+L FD++F TLE KKLPDG+VEE+PDLNTGI K+ +EE
Sbjct: 480 KTTQRNLKAQFLRNSKPVGELTFDLRFFPTLEEKKLPDGSVEELPDLNTGIAKVVVEEGS 539
Query: 536 DLDETGKAVNSYVELFVNSKLVLTTSTIKKSEKPSWAAPYEAVVTDRRKTRIKLVVKNDK 595
E + V +YVE+++N+KLVLTT + W + YEAV+ DRRKTR K VVK+ K
Sbjct: 540 RFAEEEQKVTAYVEVYLNAKLVLTTGKATDTGTLKWNSDYEAVIADRRKTRYKFVVKDGK 599
Query: 596 GDIISSTVQTLNDLIDRTLVAKEWIPLKNGKSSLKISTQWKPVSLDIGSNDVAYTPPVGV 655
G+ I ST+QTLNDLIDR+ V K IPLKN K +KI+T W+PV L+IGSN VAYTPP+G
Sbjct: 600 GEEIGSTIQTLNDLIDRSQVNKNLIPLKNQKGDIKITTYWRPVRLEIGSNSVAYTPPIGA 659
Query: 656 LRILLNKATGLKNLEKFGTIDPYARVLVNNLPKGRTNVVESTVNPVWNEAIYVAVSSSNQ 715
+R+ + KA L+NLEKFGTIDPY +VLVN L KGRT+ T+NPVWN+ IYVAV+S NQ
Sbjct: 660 IRVFIEKANDLRNLEKFGTIDPYCKVLVNGLSKGRTDFKSQTLNPVWNQVIYVAVTSPNQ 719
Query: 716 KVSIECLDVEYAGEDRSLGKVDIPISDMFQKGSDDKYIAHIDDEPKTGNLV-SKKGAKGV 774
+++++C+DVE +DRSLG+ ++ + D+F+K +DKY ID++ K G LV KK KG
Sbjct: 720 RITLQCMDVETVNKDRSLGEFNVNVQDLFKKDENDKYEETIDEKAKVGRLVMPKKKPKGT 779
Query: 775 ITYYASFYPAVPVLSLEEIQEMDEISKKKANWEKSKAEFEKIGDKKNVXXXXXXXXXXXX 834
ITYY SFYPA+PVL+LEEIQ++D+++KKK E K+ ++ K +
Sbjct: 780 ITYYTSFYPALPVLTLEEIQDLDKVNKKKKALELRKSAIDE--KKISKEDKAKFDQEWNE 837
Query: 835 XXXXXXMYSSKMKLDLDELLQYNSGVLSVYILSGELPQQGCYLQAFFDANGYARFVSQKS 894
MYS++ KLDL ELLQYN GVL+V +L+GELP G Y+QAFFD NG+ RFVS +
Sbjct: 838 VKELEDMYSNRQKLDLPELLQYNQGVLAVTVLNGELPDSGLYVQAFFDDNGHPRFVSPRI 897
Query: 895 PTRSFRSGMVGDVIIKELEWSTTTFRVSKDKNLNKAEDCLCEVTIPTIELLKNCYYKPSI 954
P+R ++G GDVIIKEL+ S TTFRV+K+KN N+ E C+CEV +PT EL+KNCYYKPSI
Sbjct: 898 PSRIVKNGWSGDVIIKELDKSITTFRVAKNKNYNRVEKCVCEVELPTQELVKNCYYKPSI 957
Query: 955 LSLTGPSSAKIMVQVSWFPISVTKLPQSDLITNCGDLKINIKSANDLISSDRNGKSDPFV 1014
L L+G SAK+M+Q+SWFPI +LP +DLITN GDL I +SA +LI+SD NG SDP++
Sbjct: 958 LHLSGEGSAKLMLQISWFPIDTKQLPANDLITNSGDLTIMSRSAENLIASDLNGYSDPYL 1017
Query: 1015 KLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANRVNNYLKIKIMDWDAGNKDDNIGEA 1074
K Y+N+ YKTK +KKTL+P WN+ T+Q+ NR+N+ L+IK+MDWD+ + DD IG A
Sbjct: 1018 KYYINNEEDCAYKTKVVKKTLNPKWNDEGTIQINNRLNDVLRIKVMDWDSTSADDTIGTA 1077
Query: 1075 ILPLSKIDPENPTELDIPLVLPSEKKEDAGVVHLSFEFQPRYVLAVTRKETKIGDLAGKG 1134
+PL+K+ E TELD+P+ +D G++HL+F F+PRY ++V+++E K+GD+A KG
Sbjct: 1078 EIPLNKVKVEGTTELDVPVEGLENAGQDGGMLHLAFSFKPRYTISVSKREKKVGDIASKG 1137
Query: 1135 FSAGLQAGTTVIGGGLGAV 1153
GL+AGTTVIGGG+GA+
Sbjct: 1138 LGTGLKAGTTVIGGGVGAI 1156
>YNL087w [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 1178
Score = 1251 bits (3236), Expect = 0.0
Identities = 617/1143 (53%), Positives = 819/1143 (70%), Gaps = 11/1143 (0%)
Query: 15 EQVVQVDGVDKLAPTAALSSDAGNVSEEKQVDPAKTSSEVRPVIDATYVGWKQMGGWEES 74
+Q+ + G+++ + + V + +V+ TS VR D +YVGWKQ+GGWEE
Sbjct: 11 DQISSMPGINEATKVESKNVVKDAVPIKSEVETNGTSI-VREKQDPSYVGWKQVGGWEEK 69
Query: 75 DTLTFEDELLGVNEETFLDNVIPDKAYGDWYHAVGIFFMXXXXXXXXXXXXXXXXPVFFI 134
D LT ED L+ VN++TFL N++PDK YGDWYH V I + V +
Sbjct: 70 DELTSEDLLVDVNKDTFLGNLLPDKFYGDWYHEVAILIIAGLCSFVLGYFKFSLASVLIV 129
Query: 135 ILATAVFYRTSVKKYRMSIRELAQKEFVVQKVEDDYESMEWLNSFLDKYWPIIEPSVSQI 194
+L T + YRTS KKYR S+R+LAQKE V+K+ DYES+EWLN+FLDKYWPIIEPSVSQ
Sbjct: 130 MLTTGMLYRTSSKKYRESLRDLAQKEQTVEKITSDYESVEWLNTFLDKYWPIIEPSVSQQ 189
Query: 195 VVEQVNEQIAVNEAIPAFVKALWIDRFTLGIKPPRIDLVKTFQNTELDVVVMDFGMSFTP 254
+V+ N ++ N AIP F+KA+W+D+FTLG+KPPRID +KTFQNT+ DVVVMD +SFTP
Sbjct: 190 IVDGTNTALSENVAIPKFIKAIWLDQFTLGVKPPRIDAIKTFQNTKSDVVVMDVCLSFTP 249
Query: 255 HDLSDLTSKQLRNYVNQTVVLKAKLFGLTVPVVVADIAFKARVRVRMKLMTPFPHIETVN 314
HD+SDL +KQ RNYVN VVLKAK+FG+ +PV VADI F+ VR R +LMT P +ET+N
Sbjct: 250 HDMSDLDAKQCRNYVNSNVVLKAKIFGMDIPVSVADIFFQVFVRFRFQLMTTLPLVETIN 309
Query: 315 IQFLDVPDIDFVCKLLGNTVFNWEIMSIPGLLPLARELARKYLGPLFLPPFSLQLNIPQL 374
IQ L+VP++DF+ +LLGN+VFNWEI++IPGL+ L +++A KYL P+ LPPFSLQLNIPQL
Sbjct: 310 IQLLEVPEVDFISRLLGNSVFNWEILAIPGLMRLIQKMAFKYLSPVLLPPFSLQLNIPQL 369
Query: 375 VSGSALSIGVLELTVKNAKDLKRS-NLMNISVDPYLQFSIGGRVLGKTRTVKDTLNPVWN 433
+S + L IGVLE+ VKNA L++ ++ +VDPYL F + G+++GKT+ K++ NPVWN
Sbjct: 370 LSKTGLPIGVLEIKVKNAHGLRKLVGMIKKTVDPYLTFELSGKIVGKTKVFKNSANPVWN 429
Query: 434 ESMFILLASFTDPLEITVYDKREHLKDKVLGRIYYNLSSLHDTPLQKNVSSHFIRNSKPV 493
ES++ILL SFTDPL I VYDKRE L DK +G + +NL+ LH KN HF+RNSKPV
Sbjct: 430 ESIYILLQSFTDPLTIAVYDKRETLSDKKMGTVIFNLNKLHANHYHKNEKVHFLRNSKPV 489
Query: 494 GDLFFDMKFHATLESKKLPDGTVEEVPDLNTGITKITIEEAKDLDETGKAVNSYVELFVN 553
G+L FD++F T+E KKL +G E +PD+NTGITKITI E K LDE + EL+VN
Sbjct: 490 GELTFDLRFFPTIEPKKLLNGDEEPLPDMNTGITKITIRELKGLDELSDKKFVFAELYVN 549
Query: 554 SKLVLTTSTIKKSEKPSWAAPYEAVVTDRRKTRIKLVVKNDKGDIISSTVQTLNDLIDRT 613
++LV+TT K++ W + Y +VVTDRRKT + V+K+ G +ISS+VQ LN LIDRT
Sbjct: 550 AELVMTTKKEKRTAHLKWNSDYYSVVTDRRKTICRFVLKDQSGKVISSSVQPLNHLIDRT 609
Query: 614 LVAKEWIPLKNGKSSLKISTQWKPVSLDIGSNDVAYTPPVGVLRILLNKATGLKNLEKFG 673
V KEWIPL+NGK LK++T W+PV +D+G V YT P+G+LR+ +NKA L+N + G
Sbjct: 610 EVNKEWIPLRNGKGELKVTTYWRPVDIDLGLKSVGYTTPIGMLRVFINKAENLRNPDSLG 669
Query: 674 TIDPYARVLVNNLPKGRTNVVESTVNPVWNEAIYVAVSSSNQKVSIECLDVEYAGEDRSL 733
I PYA+V VN + +GRTN T+NP+WN++IYV+V+S QKVSI+C ++ G+D +L
Sbjct: 670 KISPYAKVSVNGVARGRTNERIETLNPIWNQSIYVSVTSPLQKVSIDCFGIDTNGDDHNL 729
Query: 734 GKVDIPISDMFQKGSDDKYIAHIDDEPKTGNLVSKKGAKGVITYYASFYPAVPVLSLEEI 793
G ++I +++ K +DDKY ID+ P+TGNL+ KKG KG +TYY SFYP VPVLSLEE
Sbjct: 730 GSLNIQTQNIYHKDNDDKYTIFIDNAPRTGNLIGKKGVKGTVTYYLSFYPVVPVLSLEEA 789
Query: 794 QEMDEISKKKANWEKSKAEFEKIGDKKNVX--XXXXXXXXXXXXXXXXXMYSSKMKLDLD 851
+E+DEI++KK EK K+ D KN+ MYS KMKLDLD
Sbjct: 790 KEVDEINEKKDKLEKQKSTL----DDKNISKEEKERIKKEEFRLTEKYDMYSYKMKLDLD 845
Query: 852 ELLQYNSGVLSVYILSGELPQQGCYLQAFFDANGYARFVSQKSPTRSFRSGMVGDVIIKE 911
ELLQYN+GVL V +L GELPQ G Y+Q FFD+ GYA S K+ R+ ++G GD +IKE
Sbjct: 846 ELLQYNAGVLGVTVLGGELPQPGLYVQTFFDSCGYAAITSAKNAIRTIKTGWSGDFMIKE 905
Query: 912 LEWSTTTFRVSKDKNLNKAEDCLCEVTIPTIELLKNCYYKPSILSLTGPSSAKIMVQVSW 971
LEWS TTFRV+K K+ NKAE+ +CEV IPTIEL++NCYYKPS+L+L G SAK++VQVSW
Sbjct: 906 LEWSVTTFRVTKTKDANKAENFICEVNIPTIELVRNCYYKPSVLNLIGKKSAKLLVQVSW 965
Query: 972 FPISVTKLPQSDLITNCGDLKINIKSANDLISSDRNGKSDPFVKLYLNDNG-SPFYKTKT 1030
FP++ T+LPQSDLITN GDLKI KSA +LI ++NG SDP+V+ +LN+ SPF+KT
Sbjct: 966 FPVTATELPQSDLITNSGDLKITAKSAENLIGVNKNGYSDPYVEFFLNEKSTSPFFKTAV 1025
Query: 1031 IKKTLDPTWNESCTVQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELD 1090
KKTL+PTWNES T++V+NRVN+YL I + D+++ N + +IG+A++PLS IDPE+ T +
Sbjct: 1026 QKKTLNPTWNESKTIEVSNRVNDYLTINVKDYESTNSNRSIGKAVVPLSTIDPESDTTFN 1085
Query: 1091 IPLVLPSEKKEDAGVVHLSFEFQPRYVLAVTRKETKIGDLAGKGFSAGLQAGTTVIGGGL 1150
IPLV P K ED GV+HL FEF+PRY V ++E +G+ A KG G++AG+TV G
Sbjct: 1086 IPLVGP--KGEDGGVLHLEFEFEPRYTTNVVKREAGLGNFATKGLGTGIKAGSTVFALGT 1143
Query: 1151 GAV 1153
V
Sbjct: 1144 NVV 1146
>YML072c [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 1545
Score = 446 bits (1148), Expect = e-125
Identities = 320/1153 (27%), Positives = 553/1153 (47%), Gaps = 94/1153 (8%)
Query: 65 WKQMGGWEESDTLTFEDELLGVNEETFLDNVIPDKAYGDWYHAVGIFFMXXXXXXXXXXX 124
W+ +G + S + ++ V + L+N Y DWY +
Sbjct: 173 WQNVGEFHASGKGSPNTKMSRVIKAYILENF-----YNDWYCNIATVLGTCFFSWLFAYI 227
Query: 125 XXXXXPVFFIILATAVFYRTSVKKYRMSIRELAQKEFVVQKVEDDYESMEWLNSFLDKYW 184
+ FI L TA Y ++ +IR+ ++ V + + D ES WLNSFL K+W
Sbjct: 228 GFSWWSMIFIFLGTATVYNAEYTRFNRNIRDDLKRVTVEETLSDRVESTTWLNSFLSKFW 287
Query: 185 PIIEPSVSQIVVEQVNEQIAVNEAIPAF-VKALWIDRFTLGIKPPRIDLVKTFQNTELDV 243
I P +SQ V + VN Q+A P + + AL ID FTLG K P I +K++ T +
Sbjct: 288 VIYMPVLSQQVKDNVNPQLA--GVAPGYGIDALAIDEFTLGSKAPTIKGIKSYTKTGKNT 345
Query: 244 VVMDFGMSFTPHDLSDLTSKQLRNYVNQTVVLKAKL----FGLTVPVVVADIAFKARVRV 299
V MD+ +FTP D+SD+T+ + R +N + L L T+P++V DI ++R+
Sbjct: 346 VEMDWSFAFTPSDVSDMTATEAREKINPKISLGVTLGKSFVSKTMPILVEDINVAGKMRI 405
Query: 300 RMKLMTPFPHIETVNIQFLDVPDIDFVCKLLGNTVFNWEIMS-IPGLLPLARELARKYLG 358
+++ FP+I+ V++Q L+ P IDF K +G ++MS +PGL + + +G
Sbjct: 406 KVEFGKAFPNIKIVSLQLLEPPLIDFALKPIGGDTLGLDVMSFLPGLKSFVKNIINSNIG 465
Query: 359 PLFLPPFSLQLNIPQLVSG-SALSIGVLELTVKNAKDLKRSNLMNISVDPYLQFSIGGRV 417
P+ PP L +N+ +++ S +IGVL +T+ +A LK S+ + +VDPY+ + V
Sbjct: 466 PMLFPPNHLDINVEDIMAAQSKEAIGVLAVTIASADSLKGSDFITNTVDPYIVMTTEDAV 525
Query: 418 LGKTRTVKDTL-----NPVWNESMFILLASFTDPLEITVYDKREHLKDKVLGRIYYNLSS 472
G V+ ++ NP WNE+ ++LL + L + +D + KD V+G + +L+
Sbjct: 526 PGTDEEVRTSIKSNVKNPRWNETKYLLLNTLEQKLNLKCFDFNDVRKDTVIGDLQLDLAD 585
Query: 473 LHDTPLQKNVSSHFIRNSKPVGDLFFDMKFHATLESKKLP------------------DG 514
L P+ N ++ +K G L + + + E K +
Sbjct: 586 LLQNPVLDNQTAELRSGTKSKGILHYSLHWFPVKEDKSEEKAVERAEAKAKGKKEDENED 645
Query: 515 TVEEVPDLN-------TGITKITIEEAKDLDETGKAVNSY---VELFVNSKLVLTTSTIK 564
T E+ D N GI KIT+++ K LD T S ELF++ + V + T++
Sbjct: 646 TTEKEEDENEESSQTDVGIAKITLQKVKYLDTTSSMTGSLSPCAELFIDGQKVKSYRTLR 705
Query: 565 KSEKPSWAAPYEAVVTDRRKTRIKLVVKNDKGD---IISSTVQTLNDLIDRTLVAKEWIP 621
+ +PSW E +V + ++ L + +D+ + +I +L+D++ A+E++
Sbjct: 706 RINEPSWNETIEVLVPSKSNSKFVLKIFDDRMNGKALICEYSSSLDDIMTTLDTAQEFVK 765
Query: 622 LKNGKSSLKISTQWKPVSLD---IGSNDVAYTPPVGVLRILLNKATGLKNLEKFGTIDPY 678
+ + + + WK + + +N V + P+G +++ + A +L G +DPY
Sbjct: 766 -GSPQGDIYLDVSWKSIEMTGAFAAANSV--SEPIGCIKLDVKDAIIKGDLSGVGDVDPY 822
Query: 679 ARVLVNNLPKGRTNVVESTVNPVWNEAIYVAVSSSNQKVSIECLDVEYAGEDRSLGKVDI 738
V +N ++ T +P+++ + YV + S NQ +++E D + G+DR +G V I
Sbjct: 823 YTVSLNRRVLYKSIYHSDTDHPIFDNSTYVPIFSPNQILTLEFHDYQKIGKDRFIGSVQI 882
Query: 739 PISDMFQKG-SDDKYIAHIDDEPKTGNLVSK----KGAKGVITYYASFYPAVPVLSLEEI 793
P S++F+K KY+ + + + + L K K + ++ +F P V S EE+
Sbjct: 883 PTSNVFKKDPKSGKYVGN-NGKEEISKLKLKDHEHKVTESIVNVSTTFIPINLVYSPEEL 941
Query: 794 QEMD----EISKKKANWEKSKAEFEKIGDKKNVXXXXXXXXXXXXXXXXXXMYSSKMKLD 849
++ E+ +KK +E ++ E E+ +K + K KL
Sbjct: 942 VNVEKLEKELKEKKKKFEATQEENEQEMEKN--PKEWEVAEIEDPFDSDEKKINRKAKLS 999
Query: 850 LDELLQYNSGVLSVYILSGELPQQGCYLQAFFDANGYARFVSQKSPTRSFRSGMVGDVII 909
L+EL++ SG+LS+ IL G L YL+ D Y F+ K P++ + + ++ I
Sbjct: 1000 LNELIKQKSGILSMQILEGTLSPSSAYLEILADDISYPVFICMK-PSQGKLNSEMANIFI 1058
Query: 910 KELEWSTTTFRVSKDKNLNKAEDCLCEVTIPTIELLKNCYYKPSILSLTGPSSAKIMVQV 969
++L +S FRVSK ++D + E + T++LLK Y +P L+ G +K+ V+
Sbjct: 1059 RDLNYSKLHFRVSKKHIAKDSDDVISETSYSTLKLLKQAYEEPMWLNFNG---SKMKVRF 1115
Query: 970 SWFPISVTKLPQSDLITNCGDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTK 1029
+ P SV KLP S+ + + G L I + S + L S+DRNG SDPFV +++ND +K+
Sbjct: 1116 LYTPTSV-KLPSSESVEDTGYLNIKLISGHGLKSADRNGYSDPFVHIFVND--KKVFKSN 1172
Query: 1030 TIKKTLDPTWNESCTVQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTEL 1089
KKTLDP WNE + + +R N + ++DWD +D++G+A L +S ++
Sbjct: 1173 IKKKTLDPVWNEDAKIPILSRSKNQVIFNVLDWDRAGDNDDLGQASLDVSSLEVGKTYNW 1232
Query: 1090 DIPLVLPSEKKEDAGVVHLSFEFQPRYVL--------AVTRKETKIGDLAGKG----FSA 1137
++ L G + L F P Y+ +T K KI A
Sbjct: 1233 NLNL-------NTQGSIKLQGSFNPEYIKPSFDIVKGGITDKPMKIASGAAHATVGIAGT 1285
Query: 1138 GLQAGTTVIGGGL 1150
G+ A T V GGL
Sbjct: 1286 GIGAATGVATGGL 1298
>SPAPYUK71.03c [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 1225
Score = 443 bits (1140), Expect = e-124
Identities = 288/1012 (28%), Positives = 514/1012 (50%), Gaps = 51/1012 (5%)
Query: 133 FIILATAV-FYRTSVKKYRMSIRELAQKEFVVQKVEDDYESMEWLNSFLDKYWPIIEPSV 191
FII+A + +YR ++ +++ ++ + +K+E+D E++ WLN+FL ++W I EPS+
Sbjct: 180 FIIMAVCIQYYRICDRRVKVNFKDDYTRYLSTRKLENDSETVTWLNTFLQQFWYIFEPSL 239
Query: 192 SQIVVEQVNEQIAVNEAIPAFVKALWIDRFTLGIKPPRIDLVKTFQNTELDVVVMDFGMS 251
S+ + E + +QI ++E +P+F+ ++ + FTLG K PR+ ++++ TE D V+MD ++
Sbjct: 240 SERITE-ITDQI-LSENVPSFIDSMALSEFTLGTKSPRMGFIRSYPKTEEDTVMMDLRLA 297
Query: 252 FTPHDLSDLTSKQLRNYVNQTVVLKAK----LFGLTVPVVVADIAFKARVRVRMKLMTPF 307
F+P+D+SDLT +++ + + L K + +PV++ D++F +RV++KL+ +
Sbjct: 298 FSPNDISDLTGREIAACIKPKIALDLKIGKSIASAKMPVLIEDLSFTGNLRVKVKLIDKY 357
Query: 308 PHIETVNIQFLDVPDIDFVCKLLGNTVFNWEIMSIPGLLPLARELARKYLGPLFLPPFSL 367
P+ +TV + F + P ++ K LG F ++I +IPGL E LGP+ P
Sbjct: 358 PYAKTVGLTFTEKPVFSYILKPLGGDKFGFDIGNIPGLTTFITEQIHNTLGPMMYSPNVY 417
Query: 368 QLNIPQLVSGSAL--SIGVLELTVKNAKDLKRSNLMNISVDPYLQF-SIGGRVLGKTRTV 424
+L+I ++ + L ++G +E ++ K + +VDPY+ + RV+GK++
Sbjct: 418 ELDIESMMGAAGLNTALGAVEFKLRKGDGFKDG--LGGAVDPYVVIKNSADRVIGKSKVA 475
Query: 425 KDTLNPVWNESMFILLASFTDPLEITVYDKREHLKDKVLGRIYYNLSSLHDTPLQKNVSS 484
+T +PV+NE+ + +L SF++ L + VYD + DK+LG L++L P+ +
Sbjct: 476 HNTGSPVFNETFYSVLNSFSENLNLEVYDFNDIRSDKLLGSAVLPLATLEAMPVTNDAFV 535
Query: 485 HFIRNSKPVGDLFFDMKFHATLESKKLPDGTVEEVPDLNTGITKITIEEAKDL-DETGKA 543
K VG L +DMKFHA +PD E G+ + T+ + K+L ++ K
Sbjct: 536 ELTLKGKTVGRLNYDMKFHAV-----VPDSGEEITKVDGPGVLQFTVHQCKELSNDPSKR 590
Query: 544 VNSYVELFVNSKLVLTTSTIKKSEKPSWAAPYEAVVTDRRKTRIKLVVKNDKGDIISSTV 603
+Y +L +N+K V TT IKK+ PSW + ++ + + + + + ++ + T
Sbjct: 591 PTAYAKLIINNKEVYTTRKIKKNNNPSWEESFGTLLPEGKNATLGVQIFTEESEHPFGTA 650
Query: 604 Q-TLNDLIDRTLVAKEWIPLKNGKSS-LKISTQWKPVSLDIGS-NDVAYTPPVGVLRILL 660
+L DL T W PL++ S +++S WKP L+ S + +A P+G +RI L
Sbjct: 651 NVSLQDLFAATKTGLLWFPLQHAPSGRVRMSVMWKPAQLNNDSISSMALATPIGAIRIHL 710
Query: 661 NKATGLKNLEKFGTIDPYARVLVNNLPKGRTNVVESTVNPVWNEAIYVAVSSSNQKVSIE 720
A L + D YAR++ +N + RT V+ S VNP W+E +Y V + + ++
Sbjct: 711 RSANNLHSKIPGKKCDSYARIMSHNTKQFRTVVIASNVNPFWDEYMYAPVITKHDIFFLQ 770
Query: 721 CLDVEYAGEDRSLGKVDIPISDMFQKGSDDKYIAHIDDEPKTGNLVSKKGAKG--VITYY 778
++ +GED+ +G+ I IS+ +G + + + D T L S +G KG IT+
Sbjct: 771 VMNYNSSGEDKLIGQTPINISNFINQGENGALMEYHDPRELTVPLSSTRGIKGNATITFK 830
Query: 779 ASFYPAVPVLSLEEIQEMDEISKKKANWEKSKAEFEKIGDKKNVXXXXXXXXXXXXXXXX 838
F+P+ SL ++ K + K E + + +
Sbjct: 831 CDFFPSAVTTSLS--PDVTPAPKASSTVATDKVNIEVLPESQKTPTAVDNTSTSRGSTSV 888
Query: 839 XXMYSSKMKLDL------DELLQYNSGVLSVYILSGELPQQGCYLQAFFDANGYARFVSQ 892
K+ L + L + SG + I+S ++ + L F D + F+S
Sbjct: 889 KTSKPKKISELLMPSEAVNAALDFESGFMGFDIISYKIAKPAQELAIFLDDLPHHIFLSS 948
Query: 893 K-SPTRSFRSGMVGDVIIKELEWSTTTFR-VSKDKNLNKAEDCLCEVTIPTIELLKNCYY 950
+ T G+ I++LE+S TF+ + DK + T+ + +L+
Sbjct: 949 ALNVTGGATLHEYGNTFIRQLEYSQCTFKLLDGDKEVGSK-------TMLSRDLISKGAT 1001
Query: 951 KPSILSLTGPSSAKIMVQVSWFPISVTKLPQSDLITNCGDLKINIKSANDLISSDRNGKS 1010
KP L + P A I+V P+ V KL + ++ N G++ +++ A DL ++D NGKS
Sbjct: 1002 KP--LEIAFPDGASILVAFRLTPVPV-KLEEVEMYENMGEMTVDVIKATDLPAADSNGKS 1058
Query: 1011 DPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANRVNNYLKIKIMDWDAGNKDDN 1070
DPFV L G Y+TKT K+TL+PT+NES V++ + + DWD GNKDD+
Sbjct: 1059 DPFVVFEL--QGEEVYRTKTHKRTLNPTFNESFEVELPCKQTCNFVANVFDWDFGNKDDH 1116
Query: 1071 IGEAILPLSKIDPENPTELDIPLVLPSEKKEDAGVVHLSFEFQPRYVLAVTR 1122
+G ++ + + T +IPL + K+ GV++L P++VL R
Sbjct: 1117 LGSCVIDCKLLQQQQQTNYEIPL----DSKQ--GVLYLRITLSPKWVLRSKR 1162
>SPCC962.01 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 1429
Score = 373 bits (958), Expect = e-103
Identities = 310/1163 (26%), Positives = 531/1163 (45%), Gaps = 92/1163 (7%)
Query: 32 LSSDAGNVSE---EKQVDPAKTSSEVRPVIDAT---YVGWKQMGGWEESDTLTFEDELLG 85
+SS + NV E EK P K + DA +GWK + + D +L
Sbjct: 116 ISSKSENVPEPAGEKVSMPEKQDLQSALPSDAVSNAVIGWKSIYHASDVDVHDHFANVLS 175
Query: 86 VN-------EETFLDNVIPDKAYGDWYHAVGIFFMXXXXXXXXXXXXXXXXPVFFIILAT 138
E + L+ K G W+ + I +FFII+
Sbjct: 176 ALQWDSHRVEPSILETYSSYKLTGQWWQSTSILLAVSILSWIASKLWFRFFILFFIIITG 235
Query: 139 AVFYRTSVKKYRMSIRELAQKEFVVQKVEDDYESMEWLNSFLDKYWPIIEPSVSQIVVEQ 198
+ Y + + R +IRE +E + + DYE+M W N+ L ++W + EP +S+ V
Sbjct: 236 TIVYGSCMISVRRNIREEVVQELSKKNGDVDYETMSWFNTLLQRFWMLNEPEISKSVSTS 295
Query: 199 VNEQIAVNEAIPAFVKALWIDRFTLGIKPPRIDLVKTFQNTELDVVVMDFGMSFTPHDLS 258
V + IA E +P+F+K FTLG K PRID V+T E DVV+MD S TP+D
Sbjct: 296 VEQSIA--EYLPSFIKEAAFSTFTLGSKAPRIDRVRTHPPVERDVVLMDVDFSLTPNDNY 353
Query: 259 DLTSKQLRNYVNQTVVLKAKL-FG---LTVPVVVADIAFKARVRVRMKLMTPFPHIETVN 314
D+ L+ VN + L K FG + P+ + D+ ++R+R L + +P I+T +
Sbjct: 354 DVNDSSLKCRVNSLISLVIKFGFGKYMFSFPITIKDLRLSGKLRIRWGLSSDYPFIQTAS 413
Query: 315 IQFLDVPDIDFVCKLLGNTVFNWEIMSIPGLLPLARELARKYLGPLFLPPFSLQLNIPQL 374
FL+ P + + + + +I IPG+ E L + L P ++ +
Sbjct: 414 FSFLETPIVYANIRPIDIPFLDADIFYIPGIGQFVSEQLGLLLNSMVLWPNMFDYDLSAM 473
Query: 375 VSG--SALSIGVLELTVKNAKDLKRSNLMNISVD----PYLQFSIGGRVLGKTRTVKDTL 428
++G S ++GV+ L + +A+ R + + S+D ++ + GR G+T +T
Sbjct: 474 MAGIASGTAVGVVGLKIYSAR---RGEVSDSSIDRKPSSFITVTTSGREHGRTPIRSNTF 530
Query: 429 NPVWNESMFILLASFTDPLEITVYDKREHLKDKVL-GRIYYNLSSLHDTPLQKNVSSHFI 487
+P ++ ++++++ S DPL++++YD K +L G Y + SL++ ++ F+
Sbjct: 531 SPTFDTTIYVVINSLNDPLKLSLYDNSG--KSPILVGTTYIDPRSLYERGFIGDIYQ-FL 587
Query: 488 RNSKPVGDLFFDMKFHATLESKKLPDGTVEEVPDLNTGITKITIEEAKDLDE----TGKA 543
N+ VG + FD F +L KK DG+ E P+ + GI + + +L E T K+
Sbjct: 588 YNAVNVGSVAFDATFFPSLLPKKTMDGSKIEPPESSKGILNVNLGCVNNLTELTELTKKS 647
Query: 544 VNSYVELFVNSKLVLTTSTIKKSEKPSWAAPYEAVVTDRRKTRIKLVVKNDKGD--IISS 601
YV L+V+SK V + TIK ++ + A + + +K+ IK+ V + K I++
Sbjct: 648 SLKYV-LYVDSKEV-ASKTIKFVDRTPISLQTNAYIENNKKSSIKVAVFDVKSPEKAIAT 705
Query: 602 TVQTLNDLIDRTLVAKEWIPLKNGKSSLKISTQWKPVSLDIGSNDVAYTPP-VGVLRILL 660
L +L+ ++ +N K+++ I + W PV + + Y VGV+R+ +
Sbjct: 706 VSVPLPELLHEGYDTFHFV--ENPKATIDIESFWTPVDVVEEKSAKTYIDNLVGVMRLSV 763
Query: 661 NKATGLKNLE-KFGTIDPYARVLVNNLPKGRTNVVESTVNPVWNEAIYVAVSSSNQKVSI 719
KA L N+E DPYARV+V N RT + +NP+WNE +YV + + + + +
Sbjct: 764 IKANDLVNVELPTRKSDPYARVIVGNSVVARTVYTPNNLNPIWNEILYVPIMADTKTIDL 823
Query: 720 ECLDVEYAGEDRSLGKVDIPISDMFQ--KGSDDKYIAHI---DDEPKTGNLVSKKG--AK 772
E +D E +G DRSLG I + + K D +A E L S+KG +
Sbjct: 824 EAMDYEESGNDRSLGYASINVQKYIRNAKRLDRSALASTVFGTSEVNALTLTSRKGQSVR 883
Query: 773 GVITYYASFYPAVPVLSLEEIQEMDEISKKKANWEKSKAEFEKIGDKKNVXXXXXXXXXX 832
G I+ + P + + D SK+ + +NV
Sbjct: 884 GTISVNCDYRPCLRL-------NTDNSSKQSS---------------ENVQSATDPTTPA 921
Query: 833 XXXXXXXXMYSS-KMKLDLDELLQYNSGVLSVYILSGELPQQGCYLQAFFDANGYARFVS 891
SS + ++E LQY SG + I+S +L G L+ F D + +
Sbjct: 922 KDNSTSNAETSSITSVISVNEALQYPSGFALISIVSADLQDVGVDLRVFTDNAAFPFITT 981
Query: 892 QKSPTRSFRSGMVGDVIIKELEWSTTTFRVSKDKNLNKAEDCLCEVTIPTIELLKNCYYK 951
+ T++ R G +++EL++S TTF+++ K +CE ++ T++L+ +
Sbjct: 982 PIAKTKTPRWSSFGISMVRELQFSETTFQLT--DGAKKDPKVVCEHSVKTLDLVSEALGR 1039
Query: 952 PSILSLTGPSSA--KIMVQVSWFPISVTKLPQSDLITNCGDLKINIKSANDLISSDRNGK 1009
P + + G + + + +++ P+ +T P I N G L ++ +L D
Sbjct: 1040 PYSVEIPGSNGQLNHVRLSITYMPVPMTLNPMESYI-NSGSLHFMLQDGQNLPIGDIR-S 1097
Query: 1010 SDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANRVNNYLKIKIMDWDAGNKDD 1069
SDPFV L L NG +K+K IKK L+P WNE + V NRV + L++ DWD G K D
Sbjct: 1098 SDPFVVLKL--NGESAFKSKVIKKNLNPVWNEEADIVVQNRVLDVLELVCYDWDMGEKPD 1155
Query: 1070 NIGEAILPLSKIDPENPTELDIPLVLPSEKKEDAGVVHLSFEFQPRYVLAVTRKETKIGD 1129
+G + + L ++P ++ I L G ++ S F P + R++ +
Sbjct: 1156 VLGTSNIDLLSLEPNVESQQSIKL------DSKTGTINASLRFVPGW----HRRKAVLDV 1205
Query: 1130 LAGKGFSAGLQAGTTVIGGGLGA 1152
F G ++ GG+GA
Sbjct: 1206 TLADNFLHAANKGAKLVVGGVGA 1228
>At1g20080 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 535
Score = 125 bits (315), Expect = 3e-28
Identities = 118/499 (23%), Positives = 225/499 (44%), Gaps = 62/499 (12%)
Query: 169 DYESMEWLNSFLDKYWPIIEPSVSQIVVEQVNEQIAVNEAIPAF-VKALWIDRFTLGIKP 227
D++ ++WLN + WP ++ ++ ++ IA E IP + + ++ + TLG P
Sbjct: 65 DFDRIDWLNKLIGHMWPYMDKAICKMAKSIAKPIIA--EQIPNYKIDSVEFEMLTLGSLP 122
Query: 228 PRIDLVKTFQNTELDVVVMDFGMSFTPHDLSDLTSKQLRNYVNQTVVLKAKLFGLTVPVV 287
P +K + + +++ M+ + + N +++ AK FGL V
Sbjct: 123 PSFQGMKVYATDDKEII-MELSVKWAG---------------NPNIIVVAKAFGLKATVQ 166
Query: 288 VADIAFKARVRVRMKLMTP-FPHIETVNIQFLDVPDIDFVCKLLGNTVFNWEIMSIPGLL 346
V D+ A R+ +K + P FP + + +D P +DF KLLG ++M+IPGL
Sbjct: 167 VIDLQVYATPRITLKPLVPSFPCFANIFVSLMDKPQVDFGLKLLGA-----DVMAIPGLY 221
Query: 347 PLARELARKYLGPLFLPPFSLQLNIPQLVSGSALSIGVLELTVKNAKDLKRSNLMNISVD 406
+E+ + + ++L P +L + I +G+L + V A LK+ +L+ S D
Sbjct: 222 RFVQEIIKDQVANMYLWPKTLNVQIMDPSKAMKKPVGLLSVKVIKAIKLKKKDLLGGS-D 280
Query: 407 PYLQFSIGG-RVLGKTRTVK-DTLNPVWNESM-FILLASFTDPLEITVYDKREHLKDKVL 463
PY++ ++ G +V GK VK LNP WNE ++ + L++ VYD + K +
Sbjct: 281 PYVKLTLSGDKVPGKKTVVKHSNLNPEWNEEFDLVVKEPESQELQLIVYDWEQVGKHDKI 340
Query: 464 GRIYYNLSSLHDTPLQ-KNVSSHFIRNSKPVGDLFFDMKFHATLESKKLP---DGTVEEV 519
G L L TP + K ++ +++ +P + + +E + P D E +
Sbjct: 341 GMNVIQLKDL--TPEEPKLMTLELLKSMEPKEPVSEKSRGQLVVEVEYKPFKDDDIPENI 398
Query: 520 PDLNT------------GITKITIEEAKDLDETGK-AVNSYVELFVNSKLVLTTSTIKKS 566
D N G+ + + EA+DL+ GK N V L + T +KK+
Sbjct: 399 DDPNAVEKAPEGTPSTGGLLVVIVHEAEDLE--GKYHTNPSVRLLFRGE-ERKTKRVKKN 455
Query: 567 EKPSWAAPYEAVVTD---RRKTRIKLVVKNDK----GDIISSTVQTLNDLIDRTLVAKEW 619
+P W ++ + + K ++++ + + + + V L D++ + ++
Sbjct: 456 REPRWDEDFQFPLDEPPINDKLHVEVISSSSRLIHPKETLGYVVINLGDVVSNRRINDKY 515
Query: 620 --IPLKNGKSSLKISTQWK 636
I KNG+ ++I QW+
Sbjct: 516 HLIDSKNGR--IQIELQWR 532
Score = 65.5 bits (158), Expect = 5e-10
Identities = 40/131 (30%), Positives = 62/131 (46%), Gaps = 5/131 (3%)
Query: 989 GDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVA 1048
G L + + A L D G SDP+VKL L+ + P KT L+P WNE + V
Sbjct: 258 GLLSVKVIKAIKLKKKDLLGGSDPYVKLTLSGDKVPGKKTVVKHSNLNPEWNEEFDLVVK 317
Query: 1049 NRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKE-----DA 1103
+ L++ + DW+ K D IG ++ L + PE P + + L+ E KE
Sbjct: 318 EPESQELQLIVYDWEQVGKHDKIGMNVIQLKDLTPEEPKLMTLELLKSMEPKEPVSEKSR 377
Query: 1104 GVVHLSFEFQP 1114
G + + E++P
Sbjct: 378 GQLVVEVEYKP 388
>At5g04220 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 583
Score = 108 bits (270), Expect = 5e-23
Identities = 127/535 (23%), Positives = 234/535 (43%), Gaps = 89/535 (16%)
Query: 169 DYESMEWLNSFLDKYWPIIEPSVSQIVVEQVNEQIAVNEAIPAF-VKALWIDRFTLGIKP 227
DYE ++W N F+ WP ++ +V I+ V A + I F ++++ + +LG P
Sbjct: 67 DYERVDWFNKFISYMWPYLDKAVCGIIRSSVQPLFA--DYIGTFCIESIEFENLSLGTLP 124
Query: 228 PRIDLVKTFQNTELDVVVMDF-GMSFTPHDLSDLTSKQLRNYV-NQTVVLKAKLFGLTVP 285
P + K+ + L + F G+ F + +L + + N +VL K+ L +
Sbjct: 125 PTVH-GKSLSHLALVISYRCFSGVKFYETNEKELLFEPSIKWAGNPNIVLVLKVLSLRIR 183
Query: 286 VVVA--------------------------DIAFKARVRVRMKLMTP-FPHIETVNIQFL 318
V V+ D+ F A VRV +K + P FP V + +
Sbjct: 184 VQVSESETVKEWNIGISTAEYLNVLTLQLVDLQFFAIVRVALKPLLPTFPCFGMVVVSLM 243
Query: 319 DVPDIDFVCKLLGNTVFNWEIMSIPGLLPLARELARKYLGPLFLPPFSLQLNIPQLVSGS 378
+ P +DF K+LG ++MSIPGL +E ++ + ++ P L+ IP L S +
Sbjct: 244 EKPHVDFGLKVLGG-----DLMSIPGLYRYVQETIKRQVSSMYHWPQVLE--IPILDSST 296
Query: 379 A---LSIGVLELTVKNAKDLKRSNLMNISVDPYLQFSIGGRVL--GKTRTVKDTLNPVWN 433
A +G+L +++ A++L + +L+ S DPY++ S+ G L KT K LNP WN
Sbjct: 297 ASVKKPVGLLHVSILRARNLLKKDLLGTS-DPYVKLSLTGEKLPAKKTTIKKRNLNPEWN 355
Query: 434 ESMFILLAS-FTDPLEITVYDKREHLKDKVLGRIYYNLSSLHDTPLQ-------KNVSSH 485
E +++ + L++ V+D DKV G ++ + PLQ K +
Sbjct: 356 EHFKLIVKDPNSQVLQLEVFD-----WDKVGG---HDRLGMQMIPLQKINPGERKEFNLD 407
Query: 486 FIRNSKPV---------GDLFFDMKFHATLES--KKLPDGTVEEVPD-----LNTGITKI 529
I+NS V G L D+++ E K+ + E+ + G+ +
Sbjct: 408 LIKNSNVVMDSGDKKKRGRLEVDLRYVPFREESIKRRKESREEKSSEDDDFLSQAGLLSV 467
Query: 530 TIEEAKDLDETGKAVNSY-VELFVNSKLVLTTSTIKKSEKPSWAAPYEAVVTD---RRKT 585
++ AKD++ K N Y V LF K T +KK+ P W ++ + + +
Sbjct: 468 AVQSAKDVEGKKKHSNPYAVVLFRGEK--KKTKMLKKTRDPRWNEEFQFTLEEPPVKESI 525
Query: 586 RIKLVVKND-----KGDIISSTVQTLNDLIDRTLVAKEWIPLKNGKSSLKISTQW 635
R++++ K + + L+D++D + +++ + + + I +W
Sbjct: 526 RVEVMSKGTGFHFRSKEELGHVDINLDDVVDNGRINQKYHLINSRNGIIHIEIRW 580
Score = 75.1 bits (183), Expect = 6e-13
Identities = 40/116 (34%), Positives = 62/116 (52%)
Query: 989 GDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVA 1048
G L ++I A +L+ D G SDP+VKL L P KT K+ L+P WNE + V
Sbjct: 304 GLLHVSILRARNLLKKDLLGTSDPYVKLSLTGEKLPAKKTTIKKRNLNPEWNEHFKLIVK 363
Query: 1049 NRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKEDAG 1104
+ + L++++ DWD D +G ++PL KI+P E ++ L+ S D+G
Sbjct: 364 DPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQKINPGERKEFNLDLIKNSNVVMDSG 419
Score = 51.6 bits (122), Expect = 7e-06
Identities = 30/102 (29%), Positives = 50/102 (48%), Gaps = 3/102 (2%)
Query: 642 IGSNDVAYTPPVGVLRILLNKATGLKNLEKFGTIDPYARVLV--NNLPKGRTNVVESTVN 699
+ S+ + PVG+L + + +A L + GT DPY ++ + LP +T + + +N
Sbjct: 292 LDSSTASVKKPVGLLHVSILRARNLLKKDLLGTSDPYVKLSLTGEKLPAKKTTIKKRNLN 351
Query: 700 PVWNEAIYVAVSSSNQKV-SIECLDVEYAGEDRSLGKVDIPI 740
P WNE + V N +V +E D + G LG IP+
Sbjct: 352 PEWNEHFKLIVKDPNSQVLQLEVFDWDKVGGHDRLGMQMIPL 393
>ECU01g1250 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 1018
Score = 106 bits (264), Expect = 2e-22
Identities = 76/322 (23%), Positives = 148/322 (45%), Gaps = 22/322 (6%)
Query: 109 GIFFMXXXXXXXXXXXXXXXXPVFFIILATAVFYRTSVKKYRMSIRELAQKEFVVQKVED 168
G+ F+ +FF+ Y ++++ S++ L +K +
Sbjct: 62 GLIFVGSVAGYIIGKTGLSVVNLFFVGYVIFFVYNRKIERFTRSLKSLVYHSARQEKARN 121
Query: 169 DYESMEWLNSFLDKYWPIIEPSVSQIVVEQVNEQIAVNEAIPAFVKALWIDRFTLGIKPP 228
+ E++EWLN + K+W + EP +S V +QVN ++ + P F+ L + FTLG + P
Sbjct: 122 NGETVEWLNYIVKKFWEVGEPVISAQVYQQVNNELL--KVTPPFLNGLRLTEFTLGSRSP 179
Query: 229 RIDLVKTFQNTELDVVVMDFGMSFTPHDLSDLTSKQLRN---YVNQTVVLKAKL-----F 280
I+ + ++ + + + + ++ ++F P ++S L N N + L A++
Sbjct: 180 FIEGI-SYISMDGNTLAIEIDVAFVPLEISRDVVNYLENDSKNWNSKIQLSARVGTRNGI 238
Query: 281 GLTVPVVVADIAFKARVRVRMKLMTPFPHIETVNIQFLDVPDIDFVCKLLGNTVFNWEIM 340
G+ +P++V ++ FK RVRV L + I+ V + +D P+ DF L +IM
Sbjct: 239 GINLPILVKELFFKGRVRVVANLFSKNVFIKDVEVCLMDSPEFDFTLVPLKMV----DIM 294
Query: 341 SIPGLLPLARELARKYLGPLFLPPFSLQLNIPQLVSGSALSIGVLELTVKNAKDLKRSNL 400
+PGL R + L + P S+ +++ + +IGV+ L + + ++ L
Sbjct: 295 DVPGLSRWIRGIINSTLATTIVNPSSITIDVDHISKDKGKTIGVVCLQILSLENEDEERL 354
Query: 401 MNISVDPYLQFSIGGRVLGKTR 422
++ I GRV +TR
Sbjct: 355 T-------VEIDIDGRVEYQTR 369
>7301181 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 816
Score = 101 bits (252), Expect = 6e-21
Identities = 115/485 (23%), Positives = 218/485 (44%), Gaps = 67/485 (13%)
Query: 157 AQKEFVVQKVED--------DYESMEWLNSFLDKYWPIIEPSVSQIVVEQVNEQIAVNEA 208
++K+ ++ ++++ D E EWLN L + WP +V E + +A+ A
Sbjct: 101 SEKDVILARIDELPAWVYFPDVERCEWLNKILKQVWPNANHFARTLVKETIEPNVAL--A 158
Query: 209 IPAF-VKALWIDRFTLGIKPPRIDLVKTF-QNTELDVVVMDFGMSFTPHDLSDLTSKQLR 266
+ + + DR LG PPRI VK + +N + + ++MD DL + +
Sbjct: 159 LANYKMHGFRFDRIILGTIPPRIGGVKIYDKNVDRNEIIMDL-------DLFYASDCDIN 211
Query: 267 NYVNQTVVLKAKLFGLTVPVVVADIAFKARVRVRMK-LMTPFPHIETVNIQFLDVPDIDF 325
Y+ + + D VRV MK L+ P + + I FL+ P+IDF
Sbjct: 212 FYLGG------------MKGGIKDFQIHGWVRVVMKPLIRSMPLVGGLQIFFLNNPNIDF 259
Query: 326 VCKLLGNTVFNWEIMSIPGLLPLARELARKYLGPLFLPPFSLQLNIPQLVSGSALSI--- 382
N V + M +PGL L R + + +G + + P L +++ + VS AL +
Sbjct: 260 ------NLVGVIDFMDMPGLSDLLRRIIVEQIGNVMVLPNKLPISLSEEVSAVALKMPEP 313
Query: 383 -GVLELTVKNAKDLKRSNLMNI---SVDPYLQFSIGGRVLGKTRTVKDTLNPVWN----E 434
G+L + V AKDL + ++ + DPY ++G + KT+ + + +NP W+
Sbjct: 314 EGILRIHVVEAKDLMKKDISVLGKGKSDPYAIINVGAQEF-KTQIIDNNVNPKWDYWCEA 372
Query: 435 SMFILLASFTDPLEITVYDKREHLKDKVLGRIYYNLSSLHDTPLQKN-VSSHFIRNSKPV 493
++FI + F +EI + D + KD+ LGR +++S+ ++K V S
Sbjct: 373 TVFIEMGQF---VEIQLKDSDDSKKDENLGRASIDIASV----IKKGVVDSWLTLEDAKH 425
Query: 494 GDLFFDMKFH-ATLESKKLPDGTVE----EVPDLNTGITKITIEEAKDLDE--TGKAVNS 546
G L ++++ T + L +E V +++ + + I+ A+ L + + +
Sbjct: 426 GLLHVRLQWYKLTADPNDLQQILLETQLLRVTSMSSAVLSVFIDSARHLKQARSSSKPDP 485
Query: 547 YVELFVNSKLVLTTSTIKKSEKPSWAAPYEAVVTDRRKTRIKLVVKNDK-GDIISSTVQT 605
Y+ VN K T+ I + + P W + +V++ + + + + K G+ I T
Sbjct: 486 YLVCSVN-KQKQQTAMIMRDDSPVWEQGFTFLVSNPDNESLNIKIYDQKTGNDIGQYTYT 544
Query: 606 LNDLI 610
L+ L+
Sbjct: 545 LSTLL 549
Score = 56.2 bits (134), Expect = 3e-07
Identities = 35/129 (27%), Positives = 68/129 (52%), Gaps = 20/129 (15%)
Query: 965 IMVQVSWFPISVT--------KLPQSDLITNCGDLKINIKSANDLISSDRN----GKSDP 1012
+MV + PIS++ K+P+ + G L+I++ A DL+ D + GKSDP
Sbjct: 288 VMVLPNKLPISLSEEVSAVALKMPEPE-----GILRIHVVEAKDLMKKDISVLGKGKSDP 342
Query: 1013 FVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANRVNNYLKIKIMDWDAGNKDDNIG 1072
+ + + G+ +KT+ I ++P W+ C V + +++I++ D D KD+N+G
Sbjct: 343 YAIINV---GAQEFKTQIIDNNVNPKWDYWCEATVFIEMGQFVEIQLKDSDDSKKDENLG 399
Query: 1073 EAILPLSKI 1081
A + ++ +
Sbjct: 400 RASIDIASV 408
>At5g11100 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 574
Score = 98.2 bits (243), Expect = 7e-20
Identities = 139/552 (25%), Positives = 226/552 (40%), Gaps = 78/552 (14%)
Query: 139 AVFYRTSVKKYRMSIRELAQKEFVV----QKVEDDYESMEWLNSFLDKYWPIIEPSVSQI 194
A F R +V+ R + +VV QK+ + WLN L+K WP + + S++
Sbjct: 40 AAFARMTVQDSRKLLPGDFYPSWVVFSQRQKLSYSKCLLNWLNLELEKIWPYVNEAASEL 99
Query: 195 VVEQVNEQIAVNEAIPAFVKALWIDRFTLGIKPPRIDLVKTFQNTELDVVVMDFGMSFTP 254
+ V + + PA + +L +FTLG P Q T + ++ + G +
Sbjct: 100 IKSSVEP--VLEQYTPAMLASLKFSKFTLGTVAP--------QFTGVSILESESGPNGIT 149
Query: 255 HDLSDLTSKQLRNYVNQTVVLKAK-LFGLTVPVVVADIAFKARVRVRMK-LMTPFPHIET 312
+L +++ N +VL K L G+++P+ V +I F R+ K L+ FP
Sbjct: 150 MEL------EMQWDGNPKIVLDVKTLLGVSLPIEVKNIGFTGVFRLIFKPLVDEFPCFGA 203
Query: 313 VNIQFLDVPDIDFVCKLLGNTVFNWEIMSIPGLLPLARELARKYLGPLFLPPFSLQLNI- 371
++ + +DF K++G E+ SIPG+ E R + P + I
Sbjct: 204 LSYSLREKKGLDFTLKVIGG-----ELTSIPGISDAIEETIRDAIEDSITWPVRKIIPIL 258
Query: 372 PQLVSGSALS-IGVLELTVKNAKDLKRSNLMNISVDPYLQFSIGGRVLG----KTRTVKD 426
P S L +G L++ V AKDL +++ S DPY I R L KT+T+ +
Sbjct: 259 PGDYSDLELKPVGKLDVKVVQAKDLANKDMIGKS-DPYAIVFI--RPLPDRTKKTKTISN 315
Query: 427 TLNPVWNESM-FILLASFTDPLEITVYDKREHLKDKVLGRIYYNLSSL-----HDTPLQK 480
+LNP+WNE FI+ T L + V+D +++G L+ L D L+
Sbjct: 316 SLNPIWNEHFEFIVEDVSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGKVKDIWLKL 375
Query: 481 NVSSHFIRNSKPVGDLF---------------FDMKFHATLESKKL-PDGTVEEVPDLN- 523
R++K G L F+ + T+ K L P+ + D+
Sbjct: 376 VKDLEIQRDTKNRGQLELLYCPLGKEGGLKNPFNPDYSLTILEKVLKPESEDSDATDMKK 435
Query: 524 -----------TGITKITIEEAKDL---DETGKAVNSYVELFVNSKLVLTTSTIKKSEKP 569
G+ +T+ A+DL D GKA V S+ T + S P
Sbjct: 436 LVTSKKKDVIVRGVLSVTVVAAEDLPAVDFMGKADAFVVITLKKSETKSKTRVVPDSLNP 495
Query: 570 SWAAPYEAVVTDRRKTRIKLVV-KNDK--GDIISSTVQTLNDLIDRTLVAKEWIPLKNGK 626
W ++ VV D + L V +DK D I + TL ++ +EW L K
Sbjct: 496 VWNQTFDFVVEDALHDLLTLEVWDHDKFGKDKIGRVIMTLTRVMLEG-EFQEWFELDGAK 554
Query: 627 S-SLKISTQWKP 637
S L + +W P
Sbjct: 555 SGKLCVHLKWTP 566
Score = 62.4 bits (150), Expect = 4e-09
Identities = 42/152 (27%), Positives = 68/152 (44%), Gaps = 14/152 (9%)
Query: 989 GDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVA 1048
G L + + A DL + D GKSDP+ +++ KTKTI +L+P WNE V
Sbjct: 271 GKLDVKVVQAKDLANKDMIGKSDPYAIVFIRPLPDRTKKTKTISNSLNPIWNEHFEFIVE 330
Query: 1049 NRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKEDA---GV 1105
+ +L +++ D + IG A +PL+++ P ++ + LV E + D G
Sbjct: 331 DVSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGKVKDIWLKLVKDLEIQRDTKNRGQ 390
Query: 1106 VHLSF-----------EFQPRYVLAVTRKETK 1126
+ L + F P Y L + K K
Sbjct: 391 LELLYCPLGKEGGLKNPFNPDYSLTILEKVLK 422
Score = 58.5 bits (140), Expect = 6e-08
Identities = 42/183 (22%), Positives = 83/183 (44%), Gaps = 19/183 (10%)
Query: 582 RRKTRIKLVVKNDKGDIISSTVQTLNDLIDRTLVAKEWIPLKNGKSSLKISTQW---KPV 638
R K + +K G++ S + ++D I+ T+ + +++ S W K +
Sbjct: 209 REKKGLDFTLKVIGGELTS--IPGISDAIEETI-----------RDAIEDSITWPVRKII 255
Query: 639 SLDIGSNDVAYTPPVGVLRILLNKATGLKNLEKFGTIDPYARVLVNNLP--KGRTNVVES 696
+ G PVG L + + +A L N + G DPYA V + LP +T + +
Sbjct: 256 PILPGDYSDLELKPVGKLDVKVVQAKDLANKDMIGKSDPYAIVFIRPLPDRTKKTKTISN 315
Query: 697 TVNPVWNEAI-YVAVSSSNQKVSIECLDVEYAGEDRSLGKVDIPISDMFQKGSDDKYIAH 755
++NP+WNE ++ S Q +++ D E G + +G +P++++ D ++
Sbjct: 316 SLNPIWNEHFEFIVEDVSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGKVKDIWLKL 375
Query: 756 IDD 758
+ D
Sbjct: 376 VKD 378
>SPCP31B10.06 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 1188
Score = 95.5 bits (236), Expect = 4e-19
Identities = 128/586 (21%), Positives = 226/586 (37%), Gaps = 118/586 (20%)
Query: 171 ESMEWLNSFLDKYWPIIEPSVSQIVVEQVNEQIAVNEAIPAFVKALWIDRFTLGIKPPRI 230
ES EW+N L+K WP+I P + V +Q+ + + +IP+FV+ + + G P R+
Sbjct: 230 ESAEWMNHLLEKVWPLINPEMFSSVADQIED--VMQASIPSFVENVRVASLDQGSHPVRV 287
Query: 231 DLVKTFQNTELDVVVMDFGMSFTPH-DLSDLTSKQLRNY----------VNQTVVLKAK- 278
+++ + E + S H D + KQ N+ V A+
Sbjct: 288 VSIRSLPSGEASESFSEKQASEAEHKDEPEQQRKQFYNFELCLAYHAKPVEDATSTSARA 347
Query: 279 ---------------LFGLTVPVVVADIAFKARVRVRMKLMTPFPHIETVNIQFLDVPDI 323
G +P+ V F AR+R R +LM P ++ V + +P++
Sbjct: 348 SNLHLRIVFYPGIKGTVGFPLPIWVEIKGFVARIRFRCELMPEVPFLKNVTFSLMGLPEL 407
Query: 324 DFVCKLLGNTVFNWEIMSIPGLLPLARELARKYLGPLFLPPFSLQLNIPQLVSGSAL--- 380
+ + N I +P + + A ++ P S+ +++ + + G +
Sbjct: 408 NVSAVPVAEGGVN--IFGLPLISKFVND-AISAAANEYVSPKSMTIDLSKTLLGDDIKKE 464
Query: 381 --SIGVLELTVKNAKDLKRSNLMNISVDPYLQ--FSIGGRVLGKTRTVKDTLNPVWNESM 436
++GV+ + + A+DL + ++ +S D Y+ F G+ L TR VK LNP+WNE
Sbjct: 465 VNALGVIFVHINRAEDLSKQDVNGLS-DAYITVGFHKFGKPLYCTRVVKQDLNPIWNEYA 523
Query: 437 FILL----ASFTDPLEITVYDKREHLKDKVLGRIYYNLSSL------------------H 474
FI + + + I ++D D V+GR L L
Sbjct: 524 FIPVFPDQVKAGEKISIELWDSDRFSPDDVVGRTKIGLHLLIQDSGKMHERCDTLTGISE 583
Query: 475 DTPLQKNV------------------SSHFIRNSKPVGDLFFDMKFHATLESKKLPDGTV 516
DT L V S H I + + D H +L++K+ + V
Sbjct: 584 DTSLPGRVFYEIGYFPRAEFKPSLKTSGHDITIPRSMRDDPAFQNPHGSLDNKE-EEAAV 642
Query: 517 EEVPD--LNTGITKITIEEAKDL-----------------------------DETGKAVN 545
PD +GI T+ +A +L +E + +
Sbjct: 643 TTAPDEEYPSGILSFTVHQAVNLQMNHPTGTFGNVSGNYNTSPAQSVGDVTAEEGSELPS 702
Query: 546 SYVELFVNSKLVLTTSTIKKSEKPSWAAPYEAVVTDRRKTRIKLVVKNDKGDIISSTVQT 605
SYV + ++ LV T T + P + A E V D R + V++ K S +
Sbjct: 703 SYVCVDLDDTLVYKTRTKVFTSNPIYNAGSEKFVKDWRNAMLCFTVRDFKLREHDSILGV 762
Query: 606 LNDLIDRTLVAK----EWIPLKN--GKSSLKISTQWKPVSLDIGSN 645
+N + TL +W P++ G S++IS ++ + L I N
Sbjct: 763 VNIPLATTLTTSSQLTKWYPIQGGIGFGSVRISILFRSMKLKIPRN 808
Score = 52.8 bits (125), Expect = 3e-06
Identities = 31/93 (33%), Positives = 49/93 (52%), Gaps = 3/93 (3%)
Query: 989 GDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQV- 1047
G + ++I A DL D NG SD ++ + + G P Y T+ +K+ L+P WNE + V
Sbjct: 469 GVIFVHINRAEDLSKQDVNGLSDAYITVGFHKFGKPLYCTRVVKQDLNPIWNEYAFIPVF 528
Query: 1048 ANRVNNYLKIKIMDWDAG--NKDDNIGEAILPL 1078
++V KI I WD+ + DD +G + L
Sbjct: 529 PDQVKAGEKISIELWDSDRFSPDDVVGRTKIGL 561
>At1g05500 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 528
Score = 92.4 bits (228), Expect = 4e-18
Identities = 126/523 (24%), Positives = 216/523 (41%), Gaps = 85/523 (16%)
Query: 171 ESMEWLNSFLDKYWPIIEPSVSQIV---VEQVNEQIAVNEAIPAFVKALWIDRFTLGIKP 227
E + WLN L K WP ++ + S+++ VE V EQ PA V +L + TLG
Sbjct: 34 ELLTWLNHHLTKIWPYVDEAASELIKASVEPVLEQYR-----PAIVASLTFSKLTLGTVA 88
Query: 228 PRIDLVKTFQNTELDVVVMDFGMSFTPHDLSDLTSK-QLRNYVNQTVVLKAK-LFGLTVP 285
P+ G+S D + +T + ++ N +VL K L G+++P
Sbjct: 89 PQFT-----------------GVSVIDGDKNGITLELDMQWDGNPNIVLGVKTLVGVSLP 131
Query: 286 VVVADIAFKARVRVRMK-LMTPFPHIETVNIQFLDVPDIDFVCKLLGNTVFNWEIMSIPG 344
+ V +I F R+ + L+ FP V++ + +DF K++G +I +IPG
Sbjct: 132 IQVKNIGFTGVFRLIFRPLVEDFPCFGAVSVSLREKKKLDFTLKVVGG-----DISAIPG 186
Query: 345 LLPLAR--------ELARKYLGPLFLPPFSLQLNIPQLVSGSALSIGVLELTVKNAKDLK 396
L ELAR+ +G L ++IP + +G+LE+ + AK+L
Sbjct: 187 LSEAIEVESYVFILELARQ-VGNLSRQLKFFCVSIPSDL--ELKPVGMLEVKLVQAKNLT 243
Query: 397 RSNLMNISVDPYLQFSIG--GRVLGKTRTVKDTLNPVWNESM-FILLASFTDPLEITVYD 453
+L+ S DP+ + I +++T+ + LNP+WNE F++ + T L + +YD
Sbjct: 244 NKDLVGKS-DPFAKMFIRPLREKTKRSKTINNDLNPIWNEHFEFVVEDASTQHLVVRIYD 302
Query: 454 KREHLKDKVLGRIYYNLSSLH-----DTPLQKNVSSHFIRNSKPVGDLFFDMKF------ 502
+++G L L D L+ R++K G++ ++ +
Sbjct: 303 DEGVQASELIGCAQIRLCELEPGKVKDVWLKLVKDLEIQRDTKNRGEVHLELLYIPYGSG 362
Query: 503 -----------HATLESKKLPDGTVEEVPD-------LNTGITKITIEEAKDL---DETG 541
+LE D T EE + G+ +T+ A+++ D G
Sbjct: 363 NGIVNPFVTSSMTSLERVLKNDTTDEENASSRKRKDVIVRGVLSVTVISAEEIPIQDLMG 422
Query: 542 KAVNSYVELFVNSKLVLTTSTIKKSEKPSWAAPYEAVVTDRRKTRIKLVVKNDK---GDI 598
KA V S T + S P W ++ VV D + L V + D
Sbjct: 423 KADPYVVLSMKKSGAKSKTRVVNDSLNPVWNQTFDFVVEDGLHDMLVLEVWDHDTFGKDY 482
Query: 599 ISSTVQTLNDLIDRTLVAKEWIPLKNGKS-SLKISTQWKPVSL 640
I + TL +I K+W PL K+ L++ +W S+
Sbjct: 483 IGRCILTLTRVIMEE-EYKDWYPLDESKTGKLQLHLKWMAQSI 524
Score = 63.5 bits (153), Expect = 2e-09
Identities = 44/151 (29%), Positives = 75/151 (49%), Gaps = 8/151 (5%)
Query: 968 QVSWFPISVTKLPQSDL-ITNCGDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFY 1026
Q+ +F +S+ SDL + G L++ + A +L + D GKSDPF K+++
Sbjct: 212 QLKFFCVSIP----SDLELKPVGMLEVKLVQAKNLTNKDLVGKSDPFAKMFIRPLREKTK 267
Query: 1027 KTKTIKKTLDPTWNESCTVQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENP 1086
++KTI L+P WNE V + +L ++I D + + IG A + L +++P
Sbjct: 268 RSKTINNDLNPIWNEHFEFVVEDASTQHLVVRIYDDEGVQASELIGCAQIRLCELEPGKV 327
Query: 1087 TELDIPLVLPSEKKEDA---GVVHLSFEFQP 1114
++ + LV E + D G VHL + P
Sbjct: 328 KDVWLKLVKDLEIQRDTKNRGEVHLELLYIP 358
Score = 55.8 bits (133), Expect = 4e-07
Identities = 38/128 (29%), Positives = 66/128 (50%), Gaps = 5/128 (3%)
Query: 989 GDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVA 1048
G L + + SA ++ D GK+DP+V L + +G+ KT+ + +L+P WN++ V
Sbjct: 403 GVLSVTVISAEEIPIQDLMGKADPYVVLSMKKSGAKS-KTRVVNDSLNPVWNQTFDFVVE 461
Query: 1049 NRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKEDAGVVHL 1108
+ +++ L +++ D D K D IG IL L+++ E + PL E K +HL
Sbjct: 462 DGLHDMLVLEVWDHDTFGK-DYIGRCILTLTRVIMEEEYKDWYPL---DESKTGKLQLHL 517
Query: 1109 SFEFQPRY 1116
+ Q Y
Sbjct: 518 KWMAQSIY 525
>Hs14149680 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 1104
Score = 87.8 bits (216), Expect = 9e-17
Identities = 137/667 (20%), Positives = 269/667 (39%), Gaps = 119/667 (17%)
Query: 169 DYESMEWLNSFLDKYWPIIEPSVSQIVVEQVNEQIAVNEAIPAFVKALWIDRFTLGIKPP 228
D E EWLN + + WP + + +++ E V AV + P ++ R LG KP
Sbjct: 135 DVEKAEWLNKIVAQVWPFLGQYMEKLLAETVAP--AVRGSNP-HLQTFTFTRVELGEKPL 191
Query: 229 RIDLVKTFQNTELDVVVMDFGMSFTPHDLSDLTSKQLRNYVNQTVVLKAKLFGLTVPVVV 288
RI VK + +++D +S+ D+ +++ Y + V +L G+
Sbjct: 192 RIIGVKVHPGQRKEQILLDLNISYVGDVQIDV---EVKKYFCKAGVKGMQLHGV------ 242
Query: 289 ADIAFKARVRVRMK-LMTPFPHIETVNIQFLDVPDIDFVCKLLGNTVFNW----EIMSIP 343
+RV ++ L+ P + V++ F+ P +D NW ++ IP
Sbjct: 243 --------LRVILEPLIGDLPFVGAVSMFFIRRPTLD----------INWTGMTNLLDIP 284
Query: 344 GLLPLARELARKYLGP-LFLPPFSLQLNIPQLVS----GSALSIGVLELTVKNAKDLKRS 398
GL L+ + + L LP L +P L S L G++ + + A+ L
Sbjct: 285 GLSSLSDTMIMDSIAAFLVLPNRLLVPLVPDLQDVAQLRSPLPRGIIRIHLLAARGLSSK 344
Query: 399 N-----LMNISVDPYLQFSIGGRVLGKTRTVKDTLNPVWNESMFILLASFT-DPLEITVY 452
+ L+ DPY +G + +R + + LNP W E+ +++ +E+ V+
Sbjct: 345 DKYVKGLIEGKSDPYALVRLGTQTF-CSRVIDEELNPQWGETYEVMVHEVPGQEIEVEVF 403
Query: 453 DKREHLKDKVLGRIYYNLSSLHDTPLQKNVSSHFIRNSKPVGDLFFDMKFHATLESKKLP 512
DK + KD LGR+ ++ + LQ +V + G + +++ + L +
Sbjct: 404 DK-DPDKDDFLGRMKLDVGKV----LQASVLDDWFPLQGGQGQVHLRLEWLSLLSDAE-- 456
Query: 513 DGTVEEVPDLNTGITK-----------ITIEEAKD--LDETGKAVNSYVELFVNSKLVLT 559
+E+V N G++ + ++ A+D L + K N V+L + +
Sbjct: 457 --KLEQVLQWNWGVSSRPDPPSAAILVVYLDRAQDLPLKKGNKEPNPMVQLSIQD-VTQE 513
Query: 560 TSTIKKSEKPSWAAPYEAVVTDRRKTRIKLVVKNDKGDII--SSTVQTLNDLIDRTLVAK 617
+ + + P W + + D + + + VK+D + + T+ L L+
Sbjct: 514 SKAVYSTNCPVWEEAFRFFLQDPQSQELDVQVKDDSRALTLGALTLPLARLLTAPELILD 573
Query: 618 EWIPLKNGKSSLKISTQWKPVSLDIGSNDVAYTPPVG----------------------- 654
+W L + + ++ + L + S+++ + G
Sbjct: 574 QWFQLSSSGPNSRLYMKLVMRILYLDSSEICFPTVPGCPGAWDVDSENPQRGSSVDAPPR 633
Query: 655 --------------VLRILLNKATGLKNLEKF------GTIDPYARVLVNNLPKGRTNVV 694
VLRI + +A L ++F G DPY ++ + R++VV
Sbjct: 634 PCHTTPDSQFGTEHVLRIHVLEAQDLIAKDRFLGGLVKGKSDPYVKLKLAG-RSFRSHVV 692
Query: 695 ESTVNPVWNEAIYVAVSS-SNQKVSIECLDVEYAGEDRSLGKVDIPISDMFQKGSDDKYI 753
+NP WNE V V+S Q++ +E D + +D LG+ + ++ + G D+++
Sbjct: 693 REDLNPRWNEVFEVIVTSVPGQELEVEVFDKD-LDKDDFLGRCKVRLTTVLNSGFLDEWL 751
Query: 754 AHIDDEP 760
++D P
Sbjct: 752 T-LEDVP 757
Score = 58.5 bits (140), Expect = 6e-08
Identities = 67/273 (24%), Positives = 118/273 (42%), Gaps = 30/273 (10%)
Query: 380 LSIGVLELTVKNAKDLKRSNLMNISVDPYLQFSIGGRVLGKTRTVKDTLNPVWNESMFIL 439
L I VLE AKD L+ DPY++ + GR ++ V++ LNP WNE ++
Sbjct: 649 LRIHVLEAQDLIAKDRFLGGLVKGKSDPYVKLKLAGRSF-RSHVVREDLNPRWNEVFEVI 707
Query: 440 LASFT-DPLEITVYDKREHLKDKVLGRIYYNLSSLHDTPLQKNVSSHFIRNSKPVGDLFF 498
+ S LE+ V+DK + KD LGR L+++ ++S F+ + D+
Sbjct: 708 VTSVPGQELEVEVFDK-DLDKDDFLGRCKVRLTTV--------LNSGFLDEWLTLEDV-P 757
Query: 499 DMKFHATLE--SKKLPDGTVEEVPDLNT------------GITKITIEEAKDLD--ETGK 542
+ H LE + + +EEV +N+ + I +E A+DL + K
Sbjct: 758 SGRLHLRLERLTPRPTAAELEEVLQVNSLIQTQKSAELAAALLSIYMERAEDLPLRKGTK 817
Query: 543 AVNSYVELFVNSKLVLTTSTIKKSEKPSWAAPYEAVVTDRRKTRIKLVVKNDKGDIISST 602
++ Y L V T TI ++ P W ++ ++L V+ + ++ S
Sbjct: 818 HLSPYATLTVGDS-SHKTKTISQTSAPVWDESASFLIRKPHTESLELQVRGEGTGVLGSL 876
Query: 603 VQTLND-LIDRTLVAKEWIPLKNGKSSLKISTQ 634
L++ L+ L W L +G+ + + Q
Sbjct: 877 SLPLSELLVADQLCLDRWFTLSSGQGQVLLRAQ 909
Score = 54.7 bits (130), Expect = 8e-07
Identities = 49/158 (31%), Positives = 77/158 (48%), Gaps = 16/158 (10%)
Query: 991 LKINIKSANDLISSDR------NGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCT 1044
L+I++ A DLI+ DR GKSDP+VKL L +++ +++ L+P WNE
Sbjct: 649 LRIHVLEAQDLIAKDRFLGGLVKGKSDPYVKLKLAGRS---FRSHVVREDLNPRWNEVFE 705
Query: 1045 VQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKEDAG 1104
V V + L++++ D D +KDD +G + L+ + N LD L L + +G
Sbjct: 706 VIVTSVPGQELEVEVFDKDL-DKDDFLGRCKVRLTTV--LNSGFLDEWLTL---EDVPSG 759
Query: 1105 VVHLSFE-FQPRYVLAVTRKETKIGDLAGKGFSAGLQA 1141
+HL E PR A + ++ L SA L A
Sbjct: 760 RLHLRLERLTPRPTAAELEEVLQVNSLIQTQKSAELAA 797
>CE18256 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 572
Score = 84.7 bits (208), Expect = 8e-16
Identities = 82/338 (24%), Positives = 154/338 (45%), Gaps = 57/338 (16%)
Query: 156 LAQKEFVVQKVED--------DYESMEWLNSFLDKYWPII----EPSVSQIVVEQVNEQI 203
L ++E ++ +++D D E +EWLN + + WP + + ++ ++ QV Q
Sbjct: 91 LREREVIMAQLQDLPAWVQFPDTERVEWLNKVIHQLWPYVGEYTKTFMNDFIIPQVKAQ- 149
Query: 204 AVNEAIPAFVKALWIDRFTLGIKPPRIDLVKTF-QNTELDVVVMDFGMSFTPHDLSDLTS 262
+P K + +G P R+ +K + N D +++D +++ +D T
Sbjct: 150 -----MPGMFKNFKFTKMDMGDIPCRVGGIKVYTTNVGRDRIIVDMDVAYAGD--ADFT- 201
Query: 263 KQLRNYVNQTVVLKAKLFGLTVPVVVADIAFKARVRVRMKLMTPF-PHIETVNIQFLDVP 321
G T + +I F ++R +K + P+ P + V+ FL++P
Sbjct: 202 --------------VSCCGFTGG--MNNIQFSGKLRAILKPLLPYPPMVGGVSGTFLEMP 245
Query: 322 DIDFVCKLLGNTVFNWEIMSIPGLLPLARELARKYLGPLFLPPFSLQLNIPQLVSGSALS 381
+DF +G E++ +PGL+ R + + L + P + + + V + L
Sbjct: 246 KMDFNLTGMG------EMVELPGLIDAIRSVINSQIAALCVLPNEIVVPLAPDVDVTQLY 299
Query: 382 I----GVLELTVKNAKDLKRSNLMNI---SVDPYLQFSIGGRVLGKTRTVKDTLNPVWNE 434
GV+ L + AK+L+ ++ I DPY + +G + KTRT+ D LNP+WNE
Sbjct: 300 FPEPDGVVRLKIIEAKNLENRDISFIKKGKSDPYAEIQVGSQFF-KTRTIDDDLNPIWNE 358
Query: 435 SMFILLASFTD--PLEITVYDKREHLKDKVLGRIYYNL 470
F + D L I ++D+ + KD+ LGR+ +L
Sbjct: 359 -YFEAVVDQADGQKLRIELFDE-DQGKDEELGRLSVDL 394
Score = 60.5 bits (145), Expect = 2e-08
Identities = 37/121 (30%), Positives = 66/121 (53%), Gaps = 7/121 (5%)
Query: 637 PVSLDIGSNDVAYTPPVGVLRILLNKATGLKNLE----KFGTIDPYARVLVNNLPKGRTN 692
P++ D+ + + P GV+R+ + +A L+N + K G DPYA + V + +T
Sbjct: 288 PLAPDVDVTQLYFPEPDGVVRLKIIEAKNLENRDISFIKKGKSDPYAEIQVGS-QFFKTR 346
Query: 693 VVESTVNPVWNEAIYVAVSSSN-QKVSIECLDVEYAGEDRSLGKVDIPISDMFQKGSDDK 751
++ +NP+WNE V ++ QK+ IE D E G+D LG++ + + + KG+ DK
Sbjct: 347 TIDDDLNPIWNEYFEAVVDQADGQKLRIELFD-EDQGKDEELGRLSVDLKLVQAKGTIDK 405
Query: 752 Y 752
+
Sbjct: 406 W 406
Score = 54.7 bits (130), Expect = 8e-07
Identities = 39/126 (30%), Positives = 64/126 (49%), Gaps = 15/126 (11%)
Query: 974 ISVTKL--PQSDLITNCGDLKINIKSANDLISSD----RNGKSDPFVKLYLNDNGSPFYK 1027
+ VT+L P+ D G +++ I A +L + D + GKSDP+ ++ + GS F+K
Sbjct: 293 VDVTQLYFPEPD-----GVVRLKIIEAKNLENRDISFIKKGKSDPYAEIQV---GSQFFK 344
Query: 1028 TKTIKKTLDPTWNESCTVQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPT 1087
T+TI L+P WNE V L+I++ D D G KD+ +G + L + +
Sbjct: 345 TRTIDDDLNPIWNEYFEAVVDQADGQKLRIELFDEDQG-KDEELGRLSVDLKLVQAKGTI 403
Query: 1088 ELDIPL 1093
+ PL
Sbjct: 404 DKWYPL 409
>At3g61050 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 510
Score = 81.6 bits (200), Expect = 6e-15
Identities = 95/387 (24%), Positives = 171/387 (43%), Gaps = 51/387 (13%)
Query: 170 YESMEWLNSFLDKYWPIIEPSVSQIVVEQVNEQIAVNEAIPAFVKALWIDRFTLGIKPPR 229
+E ++WLN L K WP I + + ++ + V + + + P + +L + TLG P+
Sbjct: 67 FEQVKWLNKLLSKMWPYIAEAATMVIRDSV--EPLLEDYRPPGITSLKFSKLTLGNVAPK 124
Query: 230 IDLVKTFQNTELDVVVMDFGMSFTPHDLSDLTSKQLRNYVNQTVVLKAKLFGLTVPVVVA 289
I+ ++ Q+ + V MD LR + +VL ++P+ +
Sbjct: 125 IEGIRV-QSFKEGQVTMDV---------------DLRWGGDPNIVLGVTALVASIPIQLK 168
Query: 290 DIAFKARVRVRMKLMTPFPHIETVNIQFL--DVPDIDFVCKLLGNTVFNWEIMSIPGLLP 347
D+ RV +L P I V + L P ID+ K +G + + +IPGL
Sbjct: 169 DLQVFTVARVIFQLADEIPCISAVVVALLAEPKPRIDYTLKAVGGS-----LTAIPGLSD 223
Query: 348 LARELARKYLGPLFLPPFSLQLNIPQL-VSGSALSI---GVLELTVKNAKDLKRSNLMNI 403
+ + + + P + + I + V S L + G L +TV A +LK L+
Sbjct: 224 MIDDTVDTIVKDMLQWPHRIVVPIGGIPVDLSDLELKPQGKLIVTVVKATNLKNKELIGK 283
Query: 404 SVDPYLQFSIGGRVLGKTRTVKDTLNPVWNESMFILLA--SFTDPLEITVYDKREHLKDK 461
S DPY I KT+ +++ LNPVW+++ F L+A T L + V+DK + +D+
Sbjct: 284 S-DPYATIYIRPVFKYKTKAIENNLNPVWDQT-FELIAEDKETQSLTVEVFDK-DVGQDE 340
Query: 462 VLGRIYYNLSSLH-------DTPLQKNVSSHFIRNSKPVGDLFFDMKFHATLESKKLPDG 514
LG + LSSL + L ++ + +++ K G + + +H + +++
Sbjct: 341 RLGLVKLPLSSLEAGVTKELELNLLSSLDTLKVKDKKDRGSITLKVHYHEFNKEEQM--A 398
Query: 515 TVEEVPDLNTGITKITIEEAKDLDETG 541
+E+ K +EE K L E G
Sbjct: 399 ALED--------EKKIMEERKRLKEAG 417
Score = 70.9 bits (172), Expect = 1e-11
Identities = 56/204 (27%), Positives = 98/204 (47%), Gaps = 25/204 (12%)
Query: 964 KIMVQVSWFPISVTKL---PQSDLITNCGDLKINIKSANDLISSDRNGKSDPFVKLYLND 1020
+I+V + P+ ++ L PQ LI + + A +L + + GKSDP+ +Y+
Sbjct: 242 RIVVPIGGIPVDLSDLELKPQGKLI-------VTVVKATNLKNKELIGKSDPYATIYIRP 294
Query: 1021 NGSPFYKTKTIKKTLDPTWNESCTVQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSK 1080
YKTK I+ L+P W+++ + ++ L +++ D D G +D+ +G LPLS
Sbjct: 295 VFK--YKTKAIENNLNPVWDQTFELIAEDKETQSLTVEVFDKDVG-QDERLGLVKLPLSS 351
Query: 1081 IDPENPTELDIPLV-----LPSEKKEDAGVVHLS---FEFQPRYVLAVTRKETKIGDLAG 1132
++ EL++ L+ L + K+D G + L EF +A E KI +
Sbjct: 352 LEAGVTKELELNLLSSLDTLKVKDKKDRGSITLKVHYHEFNKEEQMAALEDEKKIMEERK 411
Query: 1133 KGFSAGLQAGTT----VIGGGLGA 1152
+ AG+ T ++G GLGA
Sbjct: 412 RLKEAGVIGSTMDAVGMVGSGLGA 435
Score = 67.8 bits (164), Expect = 1e-10
Identities = 62/228 (27%), Positives = 108/228 (47%), Gaps = 25/228 (10%)
Query: 584 KTRIKLVVKNDKGDIISSTVQTLNDLIDRTLVAKEWIPLKNGKSSLKISTQWKP-VSLDI 642
K RI +K G + + + L+D+ID T+ + +K QW + + I
Sbjct: 201 KPRIDYTLKAVGGSL--TAIPGLSDMIDDTV-----------DTIVKDMLQWPHRIVVPI 247
Query: 643 GSNDVAYTP----PVGVLRILLNKATGLKNLEKFGTIDPYARVLVNNLPKGRTNVVESTV 698
G V + P G L + + KAT LKN E G DPYA + + + K +T +E+ +
Sbjct: 248 GGIPVDLSDLELKPQGKLIVTVVKATNLKNKELIGKSDPYATIYIRPVFKYKTKAIENNL 307
Query: 699 NPVWNEAI-YVAVSSSNQKVSIECLDVEYAGEDRSLGKVDIPISDMFQKGSDDKYIAHID 757
NPVW++ +A Q +++E D + G+D LG V +P+S + + G + ++
Sbjct: 308 NPVWDQTFELIAEDKETQSLTVEVFDKD-VGQDERLGLVKLPLSSL-EAGVTKELELNLL 365
Query: 758 DEPKTGNLVSKKGAKGVIT---YYASFYPAVPVLSLEEIQEMDEISKK 802
T + KK +G IT +Y F + +LE+ +++ E K+
Sbjct: 366 SSLDTLKVKDKKD-RGSITLKVHYHEFNKEEQMAALEDEKKIMEERKR 412
>Hs21362014 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 692
Score = 76.6 bits (187), Expect = 2e-13
Identities = 41/125 (32%), Positives = 67/125 (52%), Gaps = 3/125 (2%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANR 1050
L I ++ L + DR G SDP+VK + G +++K I K L+P W E + V +
Sbjct: 42 LDITLRRGQSLAARDRGGTSDPYVKFKIG--GKEVFRSKIIHKNLNPVWEEKACILV-DH 98
Query: 1051 VNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKEDAGVVHLSF 1110
+ L IK+ D+D G +DD +G A L L++++ PT++ + L P D G++ LS
Sbjct: 99 LREPLYIKVFDYDFGLQDDFMGSAFLDLTQLELNRPTDVTLTLKDPHYPDHDLGIILLSV 158
Query: 1111 EFQPR 1115
P+
Sbjct: 159 ILTPK 163
Score = 75.9 bits (185), Expect = 4e-13
Identities = 97/420 (23%), Positives = 171/420 (40%), Gaps = 56/420 (13%)
Query: 385 LELTVKNAKDLKRSNLMNISVDPYLQFSIGGRVLGKTRTVKDTLNPVWNESMFILLASFT 444
L++T++ + L + S DPY++F IGG+ + +++ + LNPVW E IL+
Sbjct: 42 LDITLRRGQSLAARDRGGTS-DPYVKFKIGGKEVFRSKIIHKNLNPVWEEKACILVDHLR 100
Query: 445 DPLEITVYDKREHLKDKVLGRIYYNLSSL-------------------HDTP--LQKNVS 483
+PL I V+D L+D +G + +L+ L HD L +
Sbjct: 101 EPLYIKVFDYDFGLQDDFMGSAFLDLTQLELNRPTDVTLTLKDPHYPDHDLGIILLSVIL 160
Query: 484 SHFIRNSKPVGDLF-FDMKFHATLESKKLPDGTVEEVPDLNTGITKITIEEAKDL---DE 539
+ S+ V L K + +++ L + L GI IT+ E +DL D
Sbjct: 161 TPKEGESRDVTMLMRKSWKRSSKFQTQSLRLSDLHRKSHLWRGIVSITLIEGRDLKAMDS 220
Query: 540 TGKAVNSYVELFVNSKLVLTTSTIKKSEKPSWAAPYEAVVTDRRKTRIKLVVKN---DKG 596
G + + YV+ + + + + K+ P W ++ + + R I + + K
Sbjct: 221 NGLS-DPYVKFRLGHQ-KYKSKIMPKTLNPQWREQFDFHLYEERGGVIDITAWDKDAGKR 278
Query: 597 DIISSTVQTLNDLIDRTLVAKEWIPLKNGKSSLKI---STQWKPVSL-DIGSNDV----- 647
D Q + R K + L+ G+ L + T VS+ D+ N +
Sbjct: 279 DDFIGRCQVDLSALSREQTHKLELQLEEGEGHLVLLVTLTASATVSISDLSVNSLEDQKE 338
Query: 648 ------AYTP--------PVGVLRILLNKATGLKNLEKFGTIDPYARVLVNNLPKGRTNV 693
Y+P VG L++ + +A GL + G DP+ V +NN + T+
Sbjct: 339 REEILKKYSPLRIFHNLKDVGFLQVKVIRAEGLMAADVTGKSDPFCVVELNN-DRLLTHT 397
Query: 694 VESTVNPVWNEAIYVAVSSSNQKVSIECLDVEYAGEDRSLGKVDIPISDMFQKGSDDKYI 753
V +NP WN+ + + + + D + LGKV IP+ + Q G Y+
Sbjct: 398 VYKNLNPEWNKVFTFNIKDIHSVLEVTVYDEDRDRSADFLGKVAIPLLSI-QNGEQKAYV 456
Score = 63.2 bits (152), Expect = 2e-09
Identities = 37/105 (35%), Positives = 52/105 (49%), Gaps = 3/105 (2%)
Query: 989 GDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVA 1048
G + I + DL + D NG SDP+VK L G YK+K + KTL+P W E +
Sbjct: 203 GIVSITLIEGRDLKAMDSNGLSDPYVKFRL---GHQKYKSKIMPKTLNPQWREQFDFHLY 259
Query: 1049 NRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPL 1093
+ I D DAG +DD IG + LS + E +L++ L
Sbjct: 260 EERGGVIDITAWDKDAGKRDDFIGRCQVDLSALSREQTHKLELQL 304
Score = 53.1 bits (126), Expect = 2e-06
Identities = 60/224 (26%), Positives = 98/224 (42%), Gaps = 27/224 (12%)
Query: 369 LNIPQLVSGSALSIGVLELTVKNAKDLKRSNLMNISVDPYLQFSIGGRVLGKTRTVKDTL 428
L + L S L G++ +T+ +DLK + +S DPY++F +G + K++ + TL
Sbjct: 189 LRLSDLHRKSHLWRGIVSITLIEGRDLKAMDSNGLS-DPYVKFRLGHQKY-KSKIMPKTL 246
Query: 429 NPVWNESM-FILLASFTDPLEITVYDKREHLKDKVLGRIYYNLSSL-----HDTPLQ-KN 481
NP W E F L ++IT +DK +D +GR +LS+L H LQ +
Sbjct: 247 NPQWREQFDFHLYEERGGVIDITAWDKDAGKRDDFIGRCQVDLSALSREQTHKLELQLEE 306
Query: 482 VSSHFI-------RNSKPVGDL----FFDMKFHATLESKKLPDGTVEEVPDLNTGITKIT 530
H + + + DL D K + K P + D+ G ++
Sbjct: 307 GEGHLVLLVTLTASATVSISDLSVNSLEDQKEREEILKKYSPLRIFHNLKDV--GFLQVK 364
Query: 531 IEEAKDL---DETGKAVNSYVELFVNSKLVLTTSTIKKSEKPSW 571
+ A+ L D TGK+ V N +L+ T T+ K+ P W
Sbjct: 365 VIRAEGLMAADVTGKSDPFCVVELNNDRLL--THTVYKNLNPEW 406
Score = 53.1 bits (126), Expect = 2e-06
Identities = 29/97 (29%), Positives = 54/97 (54%), Gaps = 4/97 (4%)
Query: 985 ITNCGDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCT 1044
+ + G L++ + A L+++D GKSDPF + LN++ T T+ K L+P WN+ T
Sbjct: 355 LKDVGFLQVKVIRAEGLMAADVTGKSDPFCVVELNNDR---LLTHTVYKNLNPEWNKVFT 411
Query: 1045 VQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKI 1081
+ + +++ L++ + D D D +G+ +PL I
Sbjct: 412 FNIKD-IHSVLEVTVYDEDRDRSADFLGKVAIPLLSI 447
>At1g53590 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 706
Score = 75.1 bits (183), Expect = 6e-13
Identities = 78/329 (23%), Positives = 145/329 (43%), Gaps = 43/329 (13%)
Query: 163 VQKVEDDYESMEWLNSFLDKYWPIIEPSVSQIVVEQVNEQIAVNEAIPAFV--------K 214
V V D ES+ W+N ++K WP I +EQ+ Q + IP F+ K
Sbjct: 17 VLAVLSDSESVRWMNYAVEKIWP--------ICMEQIASQKILGPIIPWFLEKYRPWTAK 68
Query: 215 ALWIDRFTLGIKPPRIDLVKTF-QNTELDVVVMDFGMSF-TPHDLSDLTSKQLRNYVNQT 272
I +G PP + ++ Q+T D +V++ GM+F D+S + + +LR +
Sbjct: 69 KAVIQHLYMGRNPPLLTDIRVLRQSTGDDHLVLELGMNFLAADDMSAILAVKLRKRLG-- 126
Query: 273 VVLKAKLFGLTVPVVVADIAFKARVRVRMKLMTPFPHIETVNIQFLDVPDIDFVCKLLGN 332
FG+ + + + + +V + +K + +P + + + F + P K +
Sbjct: 127 -------FGMWTKLHLTGMHVEGKVLIGVKFLRRWPFLGRLRVCFAEPPYFQMTVKPI-- 177
Query: 333 TVFNWEIMSIPGLLPLARELARKYLGPLFLPPFSLQLNIPQLVSGSALS---------IG 383
++ +PG+ +L + P L +++ + VS ++ + +
Sbjct: 178 FTHGLDVAVLPGIAGWLDKLLSIAFEQTLVQPNMLVVDMEKFVSPTSENWFFVDEKEPVA 237
Query: 384 VLELTVKNAKDLKRSNLMNISVDPYLQFSIGGRVLGKTRTVKDTLNPVWNESMFILLASF 443
+ + V A DLK S+L ++ DPY++ +G KT+ K TL+P W+E I + ++
Sbjct: 238 HVLVEVFEASDLKPSDLNGLA-DPYVKGKLGAYRF-KTKIQKKTLSPKWHEEFKIPIFTW 295
Query: 444 TDP--LEITVYDKREHLKDKVLGRIYYNL 470
P L I V DK + D LG N+
Sbjct: 296 DSPSILNIEVGDK-DRFVDDTLGECSVNI 323
>At2g20990 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 488
Score = 73.9 bits (180), Expect = 1e-12
Identities = 89/395 (22%), Positives = 156/395 (38%), Gaps = 60/395 (15%)
Query: 205 VNEAIPAF-VKALWIDRFTLGIKPPRIDLVKTFQNTELDVVVMDFGMSFTPHDLSDLTSK 263
+ E IP + + ++ + TLG PP +K + E ++++
Sbjct: 84 IEEQIPKYKIDSVEFETLTLGSLPPTFQGMKVYLTDEKELIM----------------EP 127
Query: 264 QLRNYVNQTVVLKAKLFGLTVPVVVADIAFKARVRVRMKLMTP-FPHIETVNIQFLDVPD 322
L+ N +++ K FGL V V D+ A+ R+ +K + P FP + + ++ P
Sbjct: 128 CLKWAANPNILVAIKAFGLKATVQVVDLQVFAQPRITLKPLVPSFPCFANIYVSLMEKPH 187
Query: 323 IDFVCKLLGNTVFNWEIMSIPGLLPLARELARKYLGPLFLPPFSLQLNIPQLVSGSALSI 382
+DF KL G ++MSIPGL ++ L F + N +
Sbjct: 188 VDFGLKLGGA-----DLMSIPGLYRFV-QVDIFLLHTSFKSLTCMSKNFFMRERAFRRPV 241
Query: 383 GVLELTVKNAKDLKRSNLMNISVDPYLQFSIG-GRVLGKTRTVK-DTLNPVWNESM-FIL 439
G++ + V A L++ +LM DP+++ + ++ K TVK LNP WNE F +
Sbjct: 242 GIVHVKVVRAVGLRKKDLMG-GADPFVKIKLSEDKIPSKKTTVKHKNLNPEWNEEFKFSV 300
Query: 440 LASFTDPLEITVYDKREHLKDKVLGRIYYNLSSLHDTPLQKNVSSHFIRNS--------- 490
T LE +VYD + + +G L + P + + +R +
Sbjct: 301 RDPQTQVLEFSVYDWEQVGNPEKMGMNVLALKEM--VPDEHKAFTLELRKTLDGGEDGQP 358
Query: 491 --------------KPVGDLFFDMKFHATLESKKLPDGTVEEVPDLNTGITKITIEEAKD 536
KP + F T +K P+GT G+ + + A+D
Sbjct: 359 PDKYRGKLEVELLYKPFTEEEMPKGFEETQAVQKAPEGT-----PAAGGMLVVIVHSAED 413
Query: 537 LDETGKAVNSYVELFVNSKLVLTTSTIKKSEKPSW 571
+ E N YV ++ + T +KK+ P W
Sbjct: 414 V-EGKHHTNPYVRIYFKGE-ERKTKHVKKNRDPRW 446
Score = 55.5 bits (132), Expect = 5e-07
Identities = 31/114 (27%), Positives = 52/114 (45%)
Query: 989 GDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVA 1048
G + + + A L D G +DPFVK+ L+++ P KT K L+P WNE V
Sbjct: 242 GIVHVKVVRAVGLRKKDLMGGADPFVKIKLSEDKIPSKKTTVKHKNLNPEWNEEFKFSVR 301
Query: 1049 NRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKED 1102
+ L+ + DW+ + +G +L L ++ P+ + L + ED
Sbjct: 302 DPQTQVLEFSVYDWEQVGNPEKMGMNVLALKEMVPDEHKAFTLELRKTLDGGED 355
>Hs6005997 [U] KOG1013 Synaptic vesicle protein rabphilin-3A
Length = 412
Score = 71.6 bits (174), Expect = 7e-12
Identities = 51/149 (34%), Positives = 75/149 (50%), Gaps = 12/149 (8%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYK--TKTIKKTLDPTWNESCT---V 1045
L I A L D NG +DP+VKL+L S K TKT++ TL+PTWNE+ T +
Sbjct: 143 LHCTITKAKGLKPMDHNGLADPYVKLHLLPGASKANKLRTKTLRNTLNPTWNETLTYYGI 202
Query: 1046 QVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPL--VLPSEKKEDA 1103
+ + L+I + D D ++ IGE +PL K+ P + +I L LP +K ED
Sbjct: 203 TDEDMIRKTLRISVCDEDKFRHNEFIGETRVPLKKLKPNHTKTFNICLEKQLPVDKTEDK 262
Query: 1104 GVVHLSFEFQPRYVLAVTRKETKIGDLAG 1132
S E + R ++++ K G L G
Sbjct: 263 -----SLEERGRILISLKYSSQKQGLLVG 286
>Hs18584396 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 562
Score = 68.6 bits (166), Expect = 6e-11
Identities = 41/119 (34%), Positives = 67/119 (55%), Gaps = 4/119 (3%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANR 1050
L I++K +L+ DR G SDP+VK L NG YK+K I K L+P W+E + + +
Sbjct: 343 LTIHLKEGRNLVVRDRCGTSDPYVKFKL--NGKTLYKSKVIYKNLNPVWDEIVVLPIQS- 399
Query: 1051 VNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKEDAGVVHLS 1109
++ L++K+ D D D +G A + LS ++ TE + L P+ ++D GV+ L+
Sbjct: 400 LDQKLRVKVYDRDL-TTSDFMGSAFVILSDLELNRTTEHILKLEDPNSLEDDMGVIVLN 457
>CE28229 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 443
Score = 68.6 bits (166), Expect = 6e-11
Identities = 51/153 (33%), Positives = 74/153 (48%), Gaps = 9/153 (5%)
Query: 989 GDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVA 1048
G L + + A DL D +G SDP+VKLYL +TK +KTL+P +NE+ +VA
Sbjct: 174 GQLTVTVIQAEDLPGMDMSGTSDPYVKLYLLPEKKKKVETKVHRKTLNPVFNETFIFKVA 233
Query: 1049 -NRVN-NYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKE---DA 1103
N + L I D+D +K D IG+ ++PL KID E + P + KE
Sbjct: 234 FNEITAKTLVFAIYDFDRFSKHDQIGQVLIPLGKIDLGAVIEEWKDIAPPPDDKEAEKSL 293
Query: 1104 GVVHLSFEFQPR----YVLAVTRKETKIGDLAG 1132
G + S + P V+ + K K D+ G
Sbjct: 294 GDICFSLRYVPTAGKLTVVILEAKNLKKMDVGG 326
>CE02711 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 441
Score = 68.6 bits (166), Expect = 6e-11
Identities = 51/153 (33%), Positives = 74/153 (48%), Gaps = 9/153 (5%)
Query: 989 GDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVA 1048
G L + + A DL D +G SDP+VKLYL +TK +KTL+P +NE+ +VA
Sbjct: 174 GQLTVTVIQAEDLPGMDMSGTSDPYVKLYLLPEKKKKVETKVHRKTLNPVFNETFIFKVA 233
Query: 1049 -NRVN-NYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKE---DA 1103
N + L I D+D +K D IG+ ++PL KID E + P + KE
Sbjct: 234 FNEITAKTLVFAIYDFDRFSKHDQIGQVLIPLGKIDLGAVIEEWKDIAPPPDDKEAEKSL 293
Query: 1104 GVVHLSFEFQPR----YVLAVTRKETKIGDLAG 1132
G + S + P V+ + K K D+ G
Sbjct: 294 GDICFSLRYVPTAGKLTVVILEAKNLKKMDVGG 326
Score = 52.0 bits (123), Expect = 5e-06
Identities = 61/236 (25%), Positives = 105/236 (43%), Gaps = 31/236 (13%)
Query: 521 DLNTGITKITIEEAKDL---DETGKAVNSYVELFV--NSKLVLTTSTIKKSEKPSWAAPY 575
D G +T+ +A+DL D +G + + YV+L++ K + T +K+ P + +
Sbjct: 170 DFQQGQLTVTVIQAEDLPGMDMSGTS-DPYVKLYLLPEKKKKVETKVHRKTLNPVFNETF 228
Query: 576 EAVVTDRRKTRIKLVV------KNDKGDIISSTVQTLNDLIDRTLVAKEW---IPLKNGK 626
V T LV + K D I + L ID V +EW P + K
Sbjct: 229 IFKVAFNEITAKTLVFAIYDFDRFSKHDQIGQVLIPLGK-IDLGAVIEEWKDIAPPPDDK 287
Query: 627 SSLKISTQWKPVSLDIGSNDVAYTPPVGVLRILLNKATGLKNLEKFGTIDPYARVLV--- 683
+ K SL + Y P G L +++ +A LK ++ G DPY ++++
Sbjct: 288 EAEK--------SLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIVLMQG 339
Query: 684 -NNLPKGRTNVVESTVNPVWNEAIYVAVSSSN-QKVS--IECLDVEYAGEDRSLGK 735
L K +T++ + T+NP +NE+ V QKVS I +D + G + ++G+
Sbjct: 340 GKRLKKKKTSIKKCTLNPYYNESFSFEVPFEQIQKVSLMITVMDYDKLGSNDAIGR 395
>Hs21361472 [O] KOG0940 Ubiquitin protein ligase RSP5/NEDD4
Length = 955
Score = 67.8 bits (164), Expect = 1e-10
Identities = 47/135 (34%), Positives = 68/135 (49%), Gaps = 11/135 (8%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKL--YLNDNGSPF--YKTKTIKKTLDPTWNESCTVQ 1046
L++ + S DL D G SDP+VKL Y+ D +TKTIKKTL+P WNE +
Sbjct: 22 LRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFR 81
Query: 1047 VANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTE------LDIPLVLPSEKK 1100
V N N+ L ++ D + +DD +G+ +PLS + E+PT D L S K
Sbjct: 82 V-NPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKS 140
Query: 1101 EDAGVVHLSFEFQPR 1115
G + L + P+
Sbjct: 141 RVKGFLRLKMAYMPK 155
Score = 55.8 bits (133), Expect = 4e-07
Identities = 29/95 (30%), Positives = 47/95 (48%), Gaps = 6/95 (6%)
Query: 655 VLRILLNKATGLKNLEKFGTIDPYARVLVNNLPKGR------TNVVESTVNPVWNEAIYV 708
+LR+ + L + FG DPY ++ + + R T ++ T+NP WNE Y
Sbjct: 21 ILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYF 80
Query: 709 AVSSSNQKVSIECLDVEYAGEDRSLGKVDIPISDM 743
V+ SN ++ E D D LG+VD+P+S +
Sbjct: 81 RVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHL 115
>7295906 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 474
Score = 67.4 bits (163), Expect = 1e-10
Identities = 49/155 (31%), Positives = 74/155 (47%), Gaps = 9/155 (5%)
Query: 987 NCGDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCT-- 1044
N L + + A +L + D G SDP+VK+YL + ++TK +KTL P +NE+ T
Sbjct: 205 NSNSLAVTVIQAEELPALDMGGTSDPYVKVYLLPDKKKKFETKVHRKTLSPVFNETFTFK 264
Query: 1045 -VQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTE--LDIPLVLPSEKKE 1101
+ A+ +N L I D+D +K D IGE +PL ID E D+ V +E
Sbjct: 265 SLPYADAMNKTLVFAIFDFDRFSKHDQIGEVKVPLCTIDLAQTIEEWRDLVSVEGEGGQE 324
Query: 1102 DAGVVHLSFEFQPR----YVLAVTRKETKIGDLAG 1132
G + S + P V+ + K K D+ G
Sbjct: 325 KLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGG 359
Score = 51.6 bits (122), Expect = 7e-06
Identities = 53/186 (28%), Positives = 89/186 (47%), Gaps = 19/186 (10%)
Query: 896 TRSFRSGMVGDVIIKELEWSTTTF-RVSKDKNLNKAEDCLCEVTIPTIELLKNCYYKPSI 954
T +F+S D + K L ++ F R SK + + + LC TI+L + +
Sbjct: 260 TFTFKSLPYADAMNKTLVFAIFDFDRFSKHDQIGEVKVPLC-----TIDLAQTIEEWRDL 314
Query: 955 LSLTGPSSAKIMVQVSWFPISVTKLPQSDLITNCGDLKINIKSANDLISSDRNGKSDPFV 1014
+S+ G + + + + S+ +P + G L + I A +L D G SDP+V
Sbjct: 315 VSVEGEGGQEKLGDICF---SLRYVPTA------GKLTVVILEAKNLKKMDVGGLSDPYV 365
Query: 1015 KLYLNDNGSPFYKTKT-IKK-TLDPTWNESCTVQVA-NRVNNY-LKIKIMDWDAGNKDDN 1070
K+ + NG K KT IKK TL+P +NES + +V ++ L + ++D+D +
Sbjct: 366 KIAIMQNGKRLKKKKTSIKKCTLNPYYNESFSFEVPFEQIQKICLVVTVVDYDRIGTSEP 425
Query: 1071 IGEAIL 1076
IG IL
Sbjct: 426 IGRCIL 431
>CE07234 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 257
Score = 66.6 bits (161), Expect = 2e-10
Identities = 39/115 (33%), Positives = 67/115 (57%), Gaps = 5/115 (4%)
Query: 1005 DRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVA-NRVNN-YLKIKIMDW 1062
DRNG SDP+VK+Y+ ++T+ I+ TL+PT+NE+ + N +++ L + + D+
Sbjct: 2 DRNGMSDPYVKVYVLPERKQKFETRIIRNTLNPTYNETFQFSIPFNELHSKTLMLVVYDY 61
Query: 1063 DAGNKDDNIGEAILPLSKIDPENPTELDIPLVLP---SEKKEDAGVVHLSFEFQP 1114
D +KDD +G+ +PL ID T+++ PL P EK+ G + S ++P
Sbjct: 62 DRLSKDDKMGQLSVPLESIDFGITTDIERPLQKPEKDDEKECRLGDICFSTRYRP 116
Score = 52.8 bits (125), Expect = 3e-06
Identities = 32/92 (34%), Positives = 50/92 (53%), Gaps = 4/92 (4%)
Query: 989 GDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIK--KTLDPTWNESCTVQ 1046
G + + I A +L D G SDP+VK+YL+ K KT + KTL+P +NES +
Sbjct: 119 GTVTLTIMEARNLKKMDVGGSSDPYVKIYLHHGRKLLSKKKTSRKYKTLNPYYNESFQFK 178
Query: 1047 VANRV--NNYLKIKIMDWDAGNKDDNIGEAIL 1076
+ + +L + + D+D +K+D IGE L
Sbjct: 179 IEPHMIEKVHLIVSVWDYDKMSKNDFIGEVTL 210
>7302558 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 983
Score = 66.2 bits (160), Expect = 3e-10
Identities = 33/103 (32%), Positives = 64/103 (62%), Gaps = 3/103 (2%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANR 1050
L++++KS +DL++ D+NG SDP+VK + G +K++TI + L+P W+E V + +
Sbjct: 255 LRVHLKSGSDLVAMDKNGLSDPYVKFKV--GGRLLHKSRTIHRDLNPVWDEVFIVPIEDP 312
Query: 1051 VNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPL 1093
+ +K+ D+D G +DD +G A L L++++ ++ + L
Sbjct: 313 FQPII-VKVFDYDWGLQDDFMGSAKLDLTQLELGKAEDIHLQL 354
Score = 57.8 bits (138), Expect = 1e-07
Identities = 30/89 (33%), Positives = 51/89 (56%), Gaps = 1/89 (1%)
Query: 385 LELTVKNAKDLKRSNLMNISVDPYLQFSIGGRVLGKTRTVKDTLNPVWNESMFILLASFT 444
L + +K+ DL + +S DPY++F +GGR+L K+RT+ LNPVW+E + +
Sbjct: 255 LRVHLKSGSDLVAMDKNGLS-DPYVKFKVGGRLLHKSRTIHRDLNPVWDEVFIVPIEDPF 313
Query: 445 DPLEITVYDKREHLKDKVLGRIYYNLSSL 473
P+ + V+D L+D +G +L+ L
Sbjct: 314 QPIIVKVFDYDWGLQDDFMGSAKLDLTQL 342
>Hs20482609 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 289
Score = 65.9 bits (159), Expect = 4e-10
Identities = 34/95 (35%), Positives = 56/95 (58%), Gaps = 3/95 (3%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQ---V 1047
L + I A +L + D +G SDPFVK+YL + +TK +K L+P WNE+ +
Sbjct: 38 LTVKIMKAQELPAKDFSGTSDPFVKIYLLPDKKHKLETKVKRKNLNPHWNETFLFEGFPY 97
Query: 1048 ANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKID 1082
V L ++++D+D +++D IGE +PL+K+D
Sbjct: 98 EKVVQRILYLQVLDYDRFSRNDPIGEVSIPLNKVD 132
>Hs14150054 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 590
Score = 64.7 bits (156), Expect = 8e-10
Identities = 48/152 (31%), Positives = 75/152 (48%), Gaps = 12/152 (7%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNES--CTVQVA 1048
L + I A DL + D NG SDP+VK+YL + ++TK +KTL+P +NE+ +V +A
Sbjct: 316 LVVRILQALDLPAKDSNGFSDPYVKIYLLPDRKKKFQTKVHRKTLNPVFNETFQFSVPLA 375
Query: 1049 NRVNNYLKIKIMDWDAGNKDDNIGEAI----LPLSKIDPENPTELDIPLVLPSEKKEDAG 1104
L + D+D ++ D IG+ + L L++ P+ P DI V +K D G
Sbjct: 376 ELAQRKLHFSVYDFDRFSRHDLIGQVVLDNLLELAEQPPDRPLWRDI--VEGGSEKADLG 433
Query: 1105 VVHLSFEFQPR----YVLAVTRKETKIGDLAG 1132
++ S + P V + K DL G
Sbjct: 434 ELNFSLCYLPTAGRLTVTIIKASNLKAMDLTG 465
>Hs4506067 [T] KOG0696 Serine/threonine protein kinase
Length = 672
Score = 64.3 bits (155), Expect = 1e-09
Identities = 35/94 (37%), Positives = 55/94 (58%), Gaps = 3/94 (3%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYL--NDNGSPFYKTKTIKKTLDPTWNESCTVQV- 1047
L + ++ A +LI D NG SDP+VKL L + KTKTI+ TL+P WNES T ++
Sbjct: 173 LHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLNPQWNESFTFKLK 232
Query: 1048 ANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKI 1081
+ + L ++I DWD ++D +G +S++
Sbjct: 233 PSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSEL 266
>Hs4507337 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 386
Score = 63.5 bits (153), Expect = 2e-09
Identities = 46/154 (29%), Positives = 77/154 (49%), Gaps = 13/154 (8%)
Query: 989 GDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCT---- 1044
G L + I A L + D G SDP+V++YL + Y+TK ++TL+P + E+
Sbjct: 123 GQLLVGILQAMGLAALDLGGSSDPYVRVYLLXDKRRRYETKVHRQTLNPHFGETFAFKVP 182
Query: 1045 -VQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPL-VLPSEKKED 1102
V++ RV L + + D+D +++D IGE +P+S +D P + L P E++E
Sbjct: 183 YVELGGRV---LVMAVYDFDRFSRNDAIGEVRVPMSSVDLGRPVQAWRELQAAPREEQEK 239
Query: 1103 AGVVHLSFEFQPR----YVLAVTRKETKIGDLAG 1132
G + S + P V+ + K K D+ G
Sbjct: 240 LGDICFSLRYVPTAGKLTVIVLEAKNLKKMDVGG 273
>At1g50260 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 778
Score = 62.4 bits (150), Expect = 4e-09
Identities = 74/330 (22%), Positives = 139/330 (41%), Gaps = 41/330 (12%)
Query: 171 ESMEWLNSFLDKYWPIIEPSVSQIVVEQVNEQIAVNEAIPAFVKALWIDRFTLGIKPPRI 230
ES+EW+N L K W + + +V + + + + P +V+ + I +F+LG +P +
Sbjct: 190 ESVEWVNMVLGKLWKVYRAGIENWLVGLL-QPVIDDLKKPDYVQRVEIKQFSLGDEPLSV 248
Query: 231 DLVKTFQNTELDVVVMDFGMSFTPHDLSDLTSKQLRNYVNQTVVLKAKLFGLTVPVVVAD 290
V+ + + + G+ +T L + LK + + VPV V D
Sbjct: 249 RNVERKTSRRANDLQYQIGLRYTGGARMLL-----------MLSLKFGVIPIVVPVGVRD 297
Query: 291 IAFKARVRVRMKLMTPFPHIETVNIQFLDVPDIDFVCKLLGNTVFN-------WEIMSIP 343
+ V+++L+ P + V+ F+ +P + F +L +FN W P
Sbjct: 298 FDIDGELWVKLRLIPTQPWVGAVSCSFVSLPKVTF--QLAAFRLFNLMGMRLSWSSDPFP 355
Query: 344 GLLPLAR-ELARKYLGP----------LFLPPFSLQLNIPQLVSGSALSIGVLELTVKNA 392
L L +L R ++ P + P L ++ G+ +G L +T+ +A
Sbjct: 356 FLTKLLTVDLPRLFVRPKKIVLDFQKGKAVGPVLEDLKSGEMQEGNKDFVGELSVTLVDA 415
Query: 393 KDLKRSNLMNISVDPYLQFSIGGRVLGKTRTVKDTL-----NPVWNESMFILLASFTDP- 446
+ L+ + DPY +G +V+ R + T+ P+WN+ L+++ +
Sbjct: 416 QKLR--YMFFGKTDPYAILRLGDQVIRSKRNSQTTVIGAPGQPIWNQDFQFLVSNPREQV 473
Query: 447 LEITVYDKREHLKDKVLGRIYYNLSSLHDT 476
L+I V D R D +G +L L DT
Sbjct: 474 LQIEVND-RLGFADMAIGTGEVDLRFLQDT 502
>7302860 [T] KOG0696 Serine/threonine protein kinase
Length = 679
Score = 62.4 bits (150), Expect = 4e-09
Identities = 37/100 (37%), Positives = 57/100 (57%), Gaps = 4/100 (4%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYL--NDNGSPFYKTKTIKKTLDPTWNESCTVQV- 1047
L + IK +LI D NG SDP+VK+ L +D KT+TIK L+P WNE+ T +
Sbjct: 191 LTVQIKEGRNLIPMDPNGLSDPYVKVKLIPDDKDQSKKKTRTIKACLNPVWNETLTYDLK 250
Query: 1048 ANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPT 1087
+ + I++ DWD +++D +G +S+I +NPT
Sbjct: 251 PEDKDRRILIEVWDWDRTSRNDFMGALSFGISEI-IKNPT 289
>Hs22043941 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 425
Score = 62.0 bits (149), Expect = 5e-09
Identities = 40/119 (33%), Positives = 64/119 (53%), Gaps = 3/119 (2%)
Query: 973 PISVTKLPQS-DLITNCGDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTI 1031
P ++ KL S D L + + A +L + D G SDP+VK++L + Y+TK
Sbjct: 99 PENLGKLQFSLDYDFQANQLTVGVLQAAELPALDMGGTSDPYVKVFLLPDKKKKYETKVH 158
Query: 1032 KKTLDPTWNESCTVQVANRV--NNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTE 1088
+KTL+P +NE+ T +V + L + I D+D +K D IGE +P++ +D P E
Sbjct: 159 RKTLNPAFNETFTFKVPYQELGGKTLVMAIYDFDRFSKHDIIGEVKVPMNTVDLGQPIE 217
>7302861 [T] KOG0696 Serine/threonine protein kinase
Length = 700
Score = 62.0 bits (149), Expect = 5e-09
Identities = 35/98 (35%), Positives = 61/98 (61%), Gaps = 3/98 (3%)
Query: 990 DLKINIKSANDLISSDRNGKSDPF--VKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQV 1047
+LK++IK A +LI D NG SDP+ V+++ + +G KTKTI+K L+P +NE+ T ++
Sbjct: 207 NLKVDIKEAANLIPMDTNGFSDPYIAVQMHPDRSGRTKKKTKTIQKNLNPVFNETFTFEL 266
Query: 1048 ANR-VNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPE 1084
+ + L I++ DWD +++D +G L ++ E
Sbjct: 267 QPQDRDKRLLIEVWDWDRTSRNDFMGSFSFSLEELQKE 304
>CE27898 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 890
Score = 61.6 bits (148), Expect = 7e-09
Identities = 40/136 (29%), Positives = 69/136 (50%), Gaps = 5/136 (3%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANR 1050
+++ +K+ DL D +G SDP+VK DN YK+ TI K L+P+W+E + + +
Sbjct: 145 MEVRLKNGEDLPVKDASGSSDPYVKFRYKDN--IVYKSGTIFKNLNPSWDEEFQM-IVDD 201
Query: 1051 VNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKEDAGVVHLSF 1110
V +++++ D+D DD +G A + +S++ PTE + L E + G V +
Sbjct: 202 VTCPIRLEVFDFDRFCTDDFMGAAEVDMSQVKWCTPTEFHVELT--DEVNQPTGRVSVCV 259
Query: 1111 EFQPRYVLAVTRKETK 1126
P L V + K
Sbjct: 260 TITPMTQLEVQQFHQK 275
Score = 54.7 bits (130), Expect = 8e-07
Identities = 32/84 (38%), Positives = 47/84 (55%), Gaps = 4/84 (4%)
Query: 998 ANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANRVNNYLKI 1057
A DL++ D GKSDPF L L + +T TI KTL P+WN+ T V + ++ L++
Sbjct: 540 AEDLVAKDFGGKSDPFAVLELVNTR---VQTNTIYKTLSPSWNKIYTFAVKD-IHTCLQV 595
Query: 1058 KIMDWDAGNKDDNIGEAILPLSKI 1081
I D D N+ + +G +PL I
Sbjct: 596 TIYDEDPNNRFEFLGRVQIPLKSI 619
>CE26302 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 372
Score = 61.6 bits (148), Expect = 7e-09
Identities = 41/126 (32%), Positives = 65/126 (51%), Gaps = 4/126 (3%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVA-N 1049
L + I A +L + D G SDP+VKL+L + +TK +K+L+P +NES T ++ N
Sbjct: 32 LTVVIIQAEELPAMDLGGTSDPYVKLFLLPDKKKKLQTKVQRKSLNPVFNESFTFKIPFN 91
Query: 1050 RVNNY-LKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKEDAGVVHL 1108
+ L + + D+D K D IG+ +PL K+D E + P E + G V L
Sbjct: 92 EIGGQTLVLNVFDFDRFGKHDQIGQISIPLGKVDLAATLERTDLIESPPENR--LGEVCL 149
Query: 1109 SFEFQP 1114
+ + P
Sbjct: 150 ALRYVP 155
>CE09100 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 804
Score = 61.6 bits (148), Expect = 7e-09
Identities = 40/136 (29%), Positives = 69/136 (50%), Gaps = 5/136 (3%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANR 1050
+++ +K+ DL D +G SDP+VK DN YK+ TI K L+P+W+E + + +
Sbjct: 59 MEVRLKNGEDLPVKDASGSSDPYVKFRYKDN--IVYKSGTIFKNLNPSWDEEFQM-IVDD 115
Query: 1051 VNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKEDAGVVHLSF 1110
V +++++ D+D DD +G A + +S++ PTE + L E + G V +
Sbjct: 116 VTCPIRLEVFDFDRFCTDDFMGAAEVDMSQVKWCTPTEFHVELT--DEVNQPTGRVSVCV 173
Query: 1111 EFQPRYVLAVTRKETK 1126
P L V + K
Sbjct: 174 TITPMTQLEVQQFHQK 189
Score = 54.7 bits (130), Expect = 8e-07
Identities = 32/84 (38%), Positives = 47/84 (55%), Gaps = 4/84 (4%)
Query: 998 ANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANRVNNYLKI 1057
A DL++ D GKSDPF L L + +T TI KTL P+WN+ T V + ++ L++
Sbjct: 454 AEDLVAKDFGGKSDPFAVLELVNTR---VQTNTIYKTLSPSWNKIYTFAVKD-IHTCLQV 509
Query: 1058 KIMDWDAGNKDDNIGEAILPLSKI 1081
I D D N+ + +G +PL I
Sbjct: 510 TIYDEDPNNRFEFLGRVQIPLKSI 533
>Hs11128045_3 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle protein
vp115/Granuphilin A contains C2 domain
Length = 149
Score = 61.2 bits (147), Expect = 9e-09
Identities = 32/108 (29%), Positives = 60/108 (54%), Gaps = 3/108 (2%)
Query: 981 QSDLITNCGDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWN 1040
+S + G L +++ A +L + NGKS+P+ ++ + GS Y T+TI+ TL+P WN
Sbjct: 13 RSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISM---GSQSYTTRTIQDTLNPKWN 69
Query: 1041 ESCTVQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTE 1088
+C + + + L + + D D + DD +G +P++KI E ++
Sbjct: 70 FNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESK 117
>Hs8922917 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 466
Score = 60.8 bits (146), Expect = 1e-08
Identities = 39/129 (30%), Positives = 69/129 (53%), Gaps = 10/129 (7%)
Query: 985 ITNCGDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCT 1044
+ + G L++ + A DL+++D +GKSDPF L L G+ +T T+ K L+P WN+ T
Sbjct: 93 VKDVGILQVKVLKAADLLAADFSGKSDPFCLLEL---GNDRLQTHTVYKNLNPEWNKVFT 149
Query: 1045 VQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPLVLPSEKKEDA- 1103
+ + +++ L++ + D D D +G+ +PL I P VL ++ E A
Sbjct: 150 FPIKD-IHDVLEVTVFDEDGDKPPDFLGKVAIPLLSIRDGQPN----CYVLKNKDLEQAF 204
Query: 1104 -GVVHLSFE 1111
GV++L +
Sbjct: 205 KGVIYLEMD 213
>CE02586 [T] KOG0696 Serine/threonine protein kinase
Length = 861
Score = 60.5 bits (145), Expect = 2e-08
Identities = 37/98 (37%), Positives = 56/98 (56%), Gaps = 4/98 (4%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYL--NDNGSPF-YKTKTIKKTLDPTWNESCTVQ- 1046
L I I A +LI D NG SDP+VK L D+G KTKT++ TL+P WNE+ T +
Sbjct: 240 LTIKILEAKNLIPMDPNGLSDPYVKCKLIPEDSGCKSKQKTKTLRATLNPQWNETFTYKL 299
Query: 1047 VANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPE 1084
+ + L I++ DWD +++D +G +S++ E
Sbjct: 300 LPGDKDRRLSIEVWDWDRTSRNDFMGSLSFGISELMKE 337
>At3g14590 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 705
Score = 60.5 bits (145), Expect = 2e-08
Identities = 80/357 (22%), Positives = 151/357 (41%), Gaps = 69/357 (19%)
Query: 139 AVFYRTSV-------KKYRMSIRELAQ----KEFVVQKVEDDYESMEWLNSFLDKYWPII 187
AVFY S+ ++Y M +R+ Q K+ ++V D ES+ WLN +++ WP
Sbjct: 27 AVFYVLSLVYLYLVHERYVMRLRKKLQFEERKQANQRRVLSDSESVRWLNHAVERIWP-- 84
Query: 188 EPSVSQIVVEQVNEQIAVNEAIPAFVKALWIDRFTLGIKPPRIDLVKTFQNTELDVVVMD 247
I +EQ+ Q + IP F +D++ +P V++
Sbjct: 85 ------ICMEQIASQKILRPIIPWF-----LDKY----RP--------------WTAVLE 115
Query: 248 FGMSF-TPHDLSDLTSKQLRNYVNQTVVLKAKLFGLTVPVVVADIAFKARVRVRMKLMTP 306
GM+F T D+S + + +LR + FG+ + + + + +V + +K +
Sbjct: 116 LGMNFLTADDMSAILAVKLRKRLG---------FGMWTKLHLTGMHVEGKVLIGVKFLRR 166
Query: 307 FPHIETVNIQFLDVPDIDFVCKLLGNTVFNWEIMSIPGLLPLARELARKYLGPLFLPPFS 366
+P + + + F + P K + T ++ +PG+ +L + P
Sbjct: 167 WPFLGRLRVCFAEPPYFQMTVKPI--TTHGLDVAVLPGIAGWLDKLLSVAFEQTLVEPNM 224
Query: 367 LQLNIPQLVSGSA----------LSIGVLELTVKNAKDLKRSNLMNISVDPYLQFSIGGR 416
L +++ + VS + + + V A D+K S+L ++ DPY++ +G
Sbjct: 225 LVVDMEKFVSPESGDNWFFVDEKEPVAHALVEVVEACDVKPSDLNGLA-DPYVKGQLGAY 283
Query: 417 VLGKTRTVKDTLNPVWNESMFILLASF--TDPLEITVYDKREHLKDKVLGRIYYNLS 471
KT+ + TL P W E I + ++ + L I V DK + D LG N++
Sbjct: 284 RF-KTKILWKTLAPKWQEEFKIPICTWDSANILNIEVQDK-DRFSDDSLGDCSVNIA 338
>Hs20127450 [T] KOG0696 Serine/threonine protein kinase
Length = 673
Score = 59.3 bits (142), Expect = 3e-08
Identities = 33/94 (35%), Positives = 55/94 (58%), Gaps = 3/94 (3%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYL--NDNGSPFYKTKTIKKTLDPTWNESCTVQV- 1047
L + ++ A +L+ D NG SDP+VKL L + KTKTIK +L+P WNE+ Q+
Sbjct: 173 LIVLVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLK 232
Query: 1048 ANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKI 1081
+ + L ++I DWD +++D +G +S++
Sbjct: 233 ESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISEL 266
>Hs13384594 [T] KOG0696 Serine/threonine protein kinase
Length = 697
Score = 59.3 bits (142), Expect = 3e-08
Identities = 31/95 (32%), Positives = 53/95 (55%), Gaps = 3/95 (3%)
Query: 990 DLKINIKSANDLISSDRNGKSDPFVKLYL--NDNGSPFYKTKTIKKTLDPTWNESCTVQV 1047
++ + + A +LI D NG SDP+VKL L + KT+T+K TL+P WNE+ +
Sbjct: 172 EIHVTVGEARNLIPMDPNGLSDPYVKLKLIPDPRNLTKQKTRTVKATLNPVWNETFVFNL 231
Query: 1048 -ANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKI 1081
V L +++ DWD +++D +G +S++
Sbjct: 232 KPGDVERRLSVEVWDWDRTSRNDFMGAMSFGVSEL 266
>At1g63220 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 147
Score = 58.9 bits (141), Expect = 4e-08
Identities = 44/163 (26%), Positives = 72/163 (43%), Gaps = 18/163 (11%)
Query: 652 PVGVLRILLNKATGLKNLEKFGTIDPYARVLVNNLPKGRTNVVEST-VNPVWNEAIYVAV 710
P G L ++L A GL++ + +DPY ++ + ++NV E P WNE V
Sbjct: 2 PHGTLEVVLVSAKGLEDADFLNNMDPYVQLTCRTQDQ-KSNVAEGMGTTPEWNETFIFTV 60
Query: 711 SSSNQKVSIECLDVEYAGEDRSLGKVDIPISDMFQKGSDDKYIAHIDDEPKTGNLVSKKG 770
S ++ + D + ED ++G+ IP+ +F +GS P N+V +
Sbjct: 61 SEGTTELKAKIFDKDVGTEDDAVGEATIPLEPVFVEGS---------IPPTAYNVVKDEE 111
Query: 771 AKGVITYYASFYPAVPVLSLEEIQEMDEISKKKANWEKSKAEF 813
KG I SF P S + MDE + W+ S+A +
Sbjct: 112 YKGEIWVALSFKP-----SENRSRGMDE--ESYGGWKNSEASY 147
>Hs5032139 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 422
Score = 58.2 bits (139), Expect = 8e-08
Identities = 45/149 (30%), Positives = 75/149 (50%), Gaps = 7/149 (4%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQV--A 1048
L + I A +L + D G SDP+VK++L + ++TK +KTL+P +NE T +V +
Sbjct: 159 LLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYS 218
Query: 1049 NRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPL-VLPSEKKEDAGVVH 1107
L + + D+D +K D IGE +P++ +D + TE L E++E G +
Sbjct: 219 ELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDIC 278
Query: 1108 LSFEFQPR----YVLAVTRKETKIGDLAG 1132
S + P V+ + K K D+ G
Sbjct: 279 FSLRYVPTAGKLTVVILEAKNLKKMDVGG 307
Score = 53.1 bits (126), Expect = 2e-06
Identities = 73/286 (25%), Positives = 120/286 (41%), Gaps = 49/286 (17%)
Query: 489 NSKPVGDLFFDMKFHATLESKK---LPDGTVEEVP---------------DLNTGITKIT 530
N K V DL MK A + L DG +E P D +
Sbjct: 103 NMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVG 162
Query: 531 IEEAKDLD--ETGKAVNSYVELFV--NSKLVLTTSTIKKSEKP------SWAAPYEAVVT 580
I +A +L + G + YV++F+ + K T +K+ P ++ PY +
Sbjct: 163 IIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSEL-- 220
Query: 581 DRRKTRIKLVVKND---KGDIISSTVQTLNDLIDRTLVAKEWIPLKNGKSSLKISTQWKP 637
KT + V D K DII +N +D V +EW L++ + +
Sbjct: 221 -GGKTLVMAVYDFDRFSKHDIIGEFKVPMNT-VDFGHVTEEWRDLQSAEKE-------EQ 271
Query: 638 VSLDIGSNDVAYTPPVGVLRILLNKATGLKNLEKFGTIDPYARV-LVNN---LPKGRTNV 693
L + Y P G L +++ +A LK ++ G DPY ++ L+ N L K +T +
Sbjct: 272 EKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTI 331
Query: 694 VESTVNPVWNEAIYVAVSSSN-QKVS--IECLDVEYAGEDRSLGKV 736
++T+NP +NE+ V QKV + LD + G++ ++GKV
Sbjct: 332 KKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKV 377
Score = 52.4 bits (124), Expect = 4e-06
Identities = 32/96 (33%), Positives = 51/96 (52%), Gaps = 4/96 (4%)
Query: 985 ITNCGDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTI--KKTLDPTWNES 1042
+ G L + I A +L D G SDP+VK++L NG K KT K TL+P +NES
Sbjct: 284 VPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNES 343
Query: 1043 CTVQVANRVNNYLK--IKIMDWDAGNKDDNIGEAIL 1076
+ +V ++ + ++D+D K+D IG+ +
Sbjct: 344 FSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFV 379
>At3g19830 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 666
Score = 58.2 bits (139), Expect = 8e-08
Identities = 77/352 (21%), Positives = 150/352 (41%), Gaps = 45/352 (12%)
Query: 171 ESMEWLNSFLDKYWPIIEPSVSQIVVEQVNEQIAVNEAIPAFVKALWIDRFTLGIKPPRI 230
ES+EW+N L K W + + +V + + + + P +V+ + I +F+LG +P +
Sbjct: 202 ESVEWVNMVLVKLWKVYRGGIENWLVGLL-QPVIDDLKKPDYVQRVEIKQFSLGDEPLSV 260
Query: 231 DLVKTFQNTELDVVVMDFGMSFTPHDLSDLTSKQLRNYVNQTVVLKAKLFGLTVPVVVAD 290
V+ + ++ + G+ +T L + LK + + VPV + D
Sbjct: 261 RNVERRTSRRVNDLQYQIGLRYTGGARMLL-----------MLSLKFGIIPVVVPVGIRD 309
Query: 291 IAFKARVRVRMKLMTPFPHIETVNIQFLDVPDIDFVCKLLGNTVFNWEIMSIPGLLPLAR 350
+ V+++L+ P + + FL KLL +P L +
Sbjct: 310 FDIDGELWVKLRLIPSAPWVGAASWAFL--------TKLLTE--------DLPRLFVRPK 353
Query: 351 ELARKYLGPLFLPPFSLQLNIPQLVSGSALSIGVLELTVKNAKDLKRSNLMNISVDPYLQ 410
++ + + P S L ++ G+ +G L +T+ NA+ K + + DPY+
Sbjct: 354 KIVLDFQKGKAVGPVSEDLKSGEMQEGNKDFVGELSVTLVNAQ--KLPYMFSGKTDPYVI 411
Query: 411 FSIGGRVLGKTRTVKDTL-----NPVWNESMFILLASFTDP-LEITVYDKREHLKDKVLG 464
IG +V+ + + T+ P+WN+ L+++ + L+I V D D +G
Sbjct: 412 LRIGDQVIRSKKNSQTTVIGAPGQPIWNQDFQFLVSNPREQVLQIEVNDCL-GFADMAIG 470
Query: 465 RIYYNLSSLHDT-PLQKNVS-----SHFIRNSKPVGDLFFDMKFHATLESKK 510
+L SL DT P + VS S F + S G++ + + A +E ++
Sbjct: 471 IGEVDLESLPDTVPTDRFVSLRGGWSLFGKGS--TGEILLRLTYKAYVEDEE 520
>7304322 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 280
Score = 58.2 bits (139), Expect = 8e-08
Identities = 29/95 (30%), Positives = 54/95 (56%), Gaps = 3/95 (3%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQ---V 1047
L + + +L + D +G SDP+V++ L + +TK ++TL+P WNE+ + +
Sbjct: 28 LILKVLQGKELPAKDLSGTSDPYVRVTLLPDKKHRLETKIKRRTLNPRWNETFYFEGFPI 87
Query: 1048 ANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKID 1082
+ L + + D+D ++DD+IGE LPL ++D
Sbjct: 88 QKLQSRVLHLHVFDYDRFSRDDSIGEVFLPLCQVD 122
>Hs22068916 [T] KOG0696 Serine/threonine protein kinase
Length = 673
Score = 57.8 bits (138), Expect = 1e-07
Identities = 32/89 (35%), Positives = 52/89 (57%), Gaps = 3/89 (3%)
Query: 996 KSANDLISSDRNGKSDPFVKLYL--NDNGSPFYKTKTIKKTLDPTWNESCTVQV-ANRVN 1052
+ A +L+ D NG SDP+VKL L + KTKTIK +L+P WNE+ Q+ + +
Sbjct: 178 RDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKD 237
Query: 1053 NYLKIKIMDWDAGNKDDNIGEAILPLSKI 1081
L ++I DWD +++D +G +S++
Sbjct: 238 RRLSVEIWDWDLTSRNDFMGSLSFGISEL 266
>CE24954 [U] KOG1013 Synaptic vesicle protein rabphilin-3A
Length = 1069
Score = 57.8 bits (138), Expect = 1e-07
Identities = 38/135 (28%), Positives = 69/135 (50%), Gaps = 14/135 (10%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYK--TKTIKKTLDPTWNESCT---V 1045
LK+++ A +L + D NG SDP+VK +L + K +KTI+KTL+P WNE + +
Sbjct: 807 LKMHLIRAKNLKAMDSNGFSDPYVKFHLLPGNTKATKLTSKTIEKTLNPEWNEEMSYYGI 866
Query: 1046 QVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPEN--------PTELDIPLVLPS 1097
++ L++ ++D D D +GE + L K++ + L +P
Sbjct: 867 TEDDKEKKILRVTVLDRDRIG-SDFLGETRIALKKLNDNEMKKFNLYLESALPVPQQTKE 925
Query: 1098 EKKEDAGVVHLSFEF 1112
E+ ED G +++ ++
Sbjct: 926 EENEDRGKINVGLQY 940
Score = 55.8 bits (133), Expect = 4e-07
Identities = 37/98 (37%), Positives = 48/98 (48%), Gaps = 4/98 (4%)
Query: 989 GDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFY--KTKTIKKTLDPTWNESC--T 1044
G L INI +L+ D G SDP+ K+ L S + KT T K+TL+P WNE
Sbjct: 945 GSLFININRCVELVGMDSTGFSDPYCKVSLTPITSKAHRAKTSTKKRTLNPEWNEQLQFV 1004
Query: 1045 VQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKID 1082
V + L+I + D D G DD IG +L S D
Sbjct: 1005 VPFKDLPKKTLQIGVYDHDLGKHDDYIGGILLSTSAKD 1042
>At1g03370_2 [S] KOG1032 Uncharacterized conserved protein contains GRAM domain
Length = 1033
Score = 57.4 bits (137), Expect = 1e-07
Identities = 44/136 (32%), Positives = 72/136 (52%), Gaps = 9/136 (6%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANR 1050
L++ + A +L + D NG SDP+V+L L S +TK +KK L+P W E + V +
Sbjct: 3 LQVRVVEARNLPAMDLNGFSDPYVRLQLGKQRS---RTKVVKKNLNPKWTEDFSFGV-DD 58
Query: 1051 VNNYLKIKIMDWDAGNKDDNIGEAILPLSKI-DPENPTELDIPLVLPSEK---KEDAGVV 1106
+N+ L + ++D D DD +G+ + +S + D EN + + L +K K+D G +
Sbjct: 59 LNDELVVSVLDEDKYFNDDFVGQVRVSVSLVFDAENQSLGTVWYPLNPKKKGSKKDCGEI 118
Query: 1107 HLSFEF-QPRYVLAVT 1121
L F Q VL +T
Sbjct: 119 LLKICFSQKNSVLDLT 134
>Hs22045984 [R] KOG1012 Ca2+-dependent lipid-binding protein CLB1/vesicle
protein vp115/Granuphilin A contains C2 domain
Length = 717
Score = 56.6 bits (135), Expect = 2e-07
Identities = 90/437 (20%), Positives = 188/437 (42%), Gaps = 59/437 (13%)
Query: 184 WPIIEPSVSQIVVEQVNEQI-AVNEAIPAFVKALWIDRFTLGIKPPRIDLVKTF-QNTEL 241
WP I + ++ E + + N + F + +G +P RI+ VK + +N +
Sbjct: 2 WPFICQFIEKLFRETIEPAVRGANTHLSTFS----FTKVDVGQQPLRINGVKVYTENVDK 57
Query: 242 DVVVMDFGMSFTPHDLSDLTSKQLRNYVNQTVVLKAKLFGLTVPVVVADIAFKARVRVRM 301
+++D +SF + DL +++ Y + V ++ G T+ V++
Sbjct: 58 RQIILDLQISFVGNCEIDL---EIKRYFCRAGVKSIQIHG-TMRVILEP----------- 102
Query: 302 KLMTPFPHIETVNIQFLDVPDIDFVCKLLGNTVFNWEIMSIPGLLPLARELARKYLGP-L 360
L+ P + ++I FL P ++ L N ++ +PGL L+ + + L
Sbjct: 103 -LIGDMPLVGALSIFFLRKPLLEINWTGLTN------LLDVPGLNGLSDTIILDIISNYL 155
Query: 361 FLP-----PFSLQLNIPQL---VSGSALSIGVLELTVKNAKDLKRSNLMNISVDPYLQFS 412
LP P ++ I QL V L I +E KD L+ DPY
Sbjct: 156 VLPNRITVPLVSEVQIAQLRFPVPKGVLRIHFIEAQDLQGKDTYLKGLVKGKSDPYGIIR 215
Query: 413 IGGRVLGKTRTVKDTLNPVWNESMFILLASFT-DPLEITVYDKREHLKDKVLGRIYYNLS 471
+G ++ ++R +K+ L+P WNE L+ LEI ++D+ + KD LG + +L
Sbjct: 216 VGNQIF-QSRVIKENLSPKWNEVYEALVYEHPGQELEIELFDE-DPDKDDFLGSLMIDLI 273
Query: 472 SLHDTPLQKNVSSHFIRNSKPVGDLFFDMKFHATLESKKLPDGTVEEV--------PDLN 523
+ ++ + F + P G L +++ + + D + ++ L+
Sbjct: 274 EVEK---ERLLDEWFTLDEVPKGKLHLRLEWLTLMPNASNLDKVLTDIKADKDQANDGLS 330
Query: 524 TGITKITIEEAKDLDETGKAV----NSYVELFVNSKLVLTTSTIK-KSEKPSWAAPYEAV 578
+ + + ++ A++L +GK + N V++ V K S I+ K+ +P W +
Sbjct: 331 SALLILYLDSARNL-PSGKKISSNPNPVVQMSVGHK--AQESKIRYKTNEPVWEENFTFF 387
Query: 579 VTDRRKTRIKLVVKNDK 595
+ + ++ +++ V++++
Sbjct: 388 IHNPKRQDLEVEVRDEQ 404
>Hs15299160 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 431
Score = 56.6 bits (135), Expect = 2e-07
Identities = 44/170 (25%), Positives = 84/170 (48%), Gaps = 14/170 (8%)
Query: 952 PSILSLTGPSSAKIMVQVSWFPISVT-KLPQSDLITNCGDLKINIKSANDL-ISSDRNGK 1009
P T PSS + V + SV P+ L+ + I+ A+ L + D+
Sbjct: 141 PGESKTTSPSSPEEDVMLGSLTFSVDYNFPKKALV-------VTIQEAHGLPVMDDQTQG 193
Query: 1010 SDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCT---VQVANRVNNYLKIKIMDWDAGN 1066
SDP++K+ + + KT+ ++KTLDP ++E+ T + + + L ++ +D +
Sbjct: 194 SDPYIKMTILPDKRHRVKTRVLRKTLDPVFDETFTFYGIPYSQLQDLVLHFLVLSFDRFS 253
Query: 1067 KDDNIGEAILPLSKIDPE-NPTELDIPLVLPS-EKKEDAGVVHLSFEFQP 1114
+DD IGE ++PL+ +DP +L ++ + +K G + +S +QP
Sbjct: 254 RDDVIGEVMVPLAGVDPSTGKVQLTRDIIKRNIQKCISRGELQVSLSYQP 303
>Hs5901958 [T] KOG2059 Ras GTPase-activating protein
Length = 826
Score = 55.8 bits (133), Expect = 4e-07
Identities = 26/91 (28%), Positives = 49/91 (53%), Gaps = 3/91 (3%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANR 1050
L+ ++ A DL DRNG SDPFV++ +T +KK+ P WNE+ ++
Sbjct: 158 LRCSVLEARDLAPKDRNGTSDPFVRVRYKGRTR---ETSIVKKSCYPRWNETFEFELQEG 214
Query: 1051 VNNYLKIKIMDWDAGNKDDNIGEAILPLSKI 1081
L ++ DWD +++D +G+ ++ + ++
Sbjct: 215 AMEALCVEAWDWDLVSRNDFLGKVVIDVQRL 245
>Hs20550102 [O] KOG0940 Ubiquitin protein ligase RSP5/NEDD4
Length = 900
Score = 55.8 bits (133), Expect = 4e-07
Identities = 41/134 (30%), Positives = 66/134 (48%), Gaps = 10/134 (7%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLND--NGS-PFYKTKTIKKTLDPTWNESCTVQV 1047
+++ + + L D G SDP+V++ L D NG +TKTIKK+L+P WNE +V
Sbjct: 21 VRVRVIAGIGLAKKDILGASDPYVRVTLYDPMNGVLTSVQTKTIKKSLNPKWNEEILFRV 80
Query: 1048 ANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENP------TELDIPLVLPSEKKE 1101
+ + L ++ D + +DD +G+ +PL + ENP T D L S K
Sbjct: 81 HPQQHRLL-FEVFDENRLTRDDFLGQVDVPLYPLPTENPRLERPYTFKDFVLHPRSHKSR 139
Query: 1102 DAGVVHLSFEFQPR 1115
G + L + P+
Sbjct: 140 VKGYLRLKMTYLPK 153
>7301258 [UT] KOG1328 Synaptic vesicle protein BAIAP3 involved in vesicle
priming/regulation
Length = 753
Score = 55.1 bits (131), Expect = 6e-07
Identities = 30/74 (40%), Positives = 39/74 (52%), Gaps = 6/74 (8%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQ---- 1046
L + I A +L+S D NG SDPFV LYL NGS Y + TL+P W E ++
Sbjct: 176 LNVEIIKAENLMSKDSNGLSDPFVTLYLESNGSHRYNSSVKPATLNPIWEEHFSLDFDAA 235
Query: 1047 --VANRVNNYLKIK 1058
V +VN L +K
Sbjct: 236 ETVKEKVNKILDVK 249
>At3g07940_2 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 165
Score = 55.1 bits (131), Expect = 6e-07
Identities = 29/99 (29%), Positives = 56/99 (56%), Gaps = 3/99 (3%)
Query: 375 VSGSALSIGVLELTVKNAKDLKRSNLMNISVDPYLQFSIGGRVLGKTRTVKDTLNPVWNE 434
++G +G++++ V +L ++M + DPY+ ++G + + KTR +K+ LNPVWNE
Sbjct: 1 MAGMVEFVGLIKVNVVKGTNLAVRDVM--TSDPYVILALGQQSV-KTRVIKNNLNPVWNE 57
Query: 435 SMFILLASFTDPLEITVYDKREHLKDKVLGRIYYNLSSL 473
++ + + PL++ VYDK D +G ++ L
Sbjct: 58 TLMLSIPEPMPPLKVLVYDKDTFSTDDFMGEAEIDIQPL 96
>Hs4503353 [U] KOG1013 Synaptic vesicle protein rabphilin-3A
Length = 400
Score = 54.7 bits (130), Expect = 8e-07
Identities = 39/118 (33%), Positives = 57/118 (48%), Gaps = 11/118 (9%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPF--YKTKTIKKTLDPTWNESCT---V 1045
L + I A L D NG +DP+VKL+L KTKT + TL+P WNE T +
Sbjct: 106 LHVCILRAKGLKPMDFNGLADPYVKLHLLPGACKANKLKTKTQRNTLNPVWNEDLTYSGI 165
Query: 1046 QVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENP------TELDIPLVLPS 1097
+ + L+I + D D + ++ IGE +PL ++ P E +PL PS
Sbjct: 166 TDDDITHKVLRIAVCDEDKLSHNEFIGEIRVPLRRLKPSQKKHFNICLERQVPLASPS 223
>Hs20552506 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 449
Score = 53.9 bits (128), Expect = 1e-06
Identities = 32/88 (36%), Positives = 49/88 (55%), Gaps = 2/88 (2%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVA-- 1048
L + I A DL + D G SDP+VK+YL + ++T+ +KTL+P ++E+ VA
Sbjct: 305 LVVKIIKALDLPAKDFTGTSDPYVKMYLLPDRKKKFQTRVHRKTLNPLFDETFQFPVAYD 364
Query: 1049 NRVNNYLKIKIMDWDAGNKDDNIGEAIL 1076
N L + D+D ++ D IGE IL
Sbjct: 365 QLSNRKLHFSVYDFDRFSRHDMIGEVIL 392
>Hs7662432 [U] KOG1013 Synaptic vesicle protein rabphilin-3A
Length = 694
Score = 53.5 bits (127), Expect = 2e-06
Identities = 38/121 (31%), Positives = 57/121 (46%), Gaps = 7/121 (5%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYK--TKTIKKTLDPTWNESCT---V 1045
L+ I A L D NG +DP+VKL+L S K TKT++ T +P WNE+ +
Sbjct: 409 LQCTIIKAKGLKPMDSNGLADPYVKLHLLPGASKSNKLRTKTLRNTRNPIWNETLVYHGI 468
Query: 1046 QVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELDIPL--VLPSEKKEDA 1103
+ L+I + D D ++ IGE L K+ P +I L V+P ++
Sbjct: 469 TDEDMQRKTLRISVCDEDKFGHNEFIGETRFSLKKLKPNQRKNFNICLERVIPMKRAGTT 528
Query: 1104 G 1104
G
Sbjct: 529 G 529
Score = 53.1 bits (126), Expect = 2e-06
Identities = 37/98 (37%), Positives = 52/98 (52%), Gaps = 4/98 (4%)
Query: 986 TNCGDLKINIKSANDLISSDRNGKSDPFVKLYLN-DNGSPF-YKTKTIKKTLDPTWNES- 1042
T G L + I L + D NG SDPFVKL+L D G +KT+ KKTL+P +NE
Sbjct: 562 TQQGGLIVGIIRCVHLAAMDANGYSDPFVKLWLKPDMGKKAKHKTQIKKKTLNPEFNEEF 621
Query: 1043 -CTVQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLS 1079
++ ++ L I + D+D G +D IG L +S
Sbjct: 622 FYDIKHSDLAKKSLDISVWDYDIGKSNDYIGGCQLGIS 659
>Hs4759026 [T] KOG2059 Ras GTPase-activating protein
Length = 804
Score = 53.1 bits (126), Expect = 2e-06
Identities = 28/82 (34%), Positives = 46/82 (55%), Gaps = 3/82 (3%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANR 1050
L+ ++ A DL D +G SDPF +++ GS +T TIKKT P W+E ++
Sbjct: 135 LRCHVLHARDLAPRDISGTSDPFARVFW---GSQSLETSTIKKTRFPHWDEVLELREMPG 191
Query: 1051 VNNYLKIKIMDWDAGNKDDNIG 1072
+ L++++ DWD K+D +G
Sbjct: 192 APSPLRVELWDWDMVGKNDFLG 213
>Hs14762054 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 425
Score = 52.8 bits (125), Expect = 3e-06
Identities = 31/94 (32%), Positives = 51/94 (53%), Gaps = 4/94 (4%)
Query: 993 INIKSANDLISSDRNGK-SDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCT---VQVA 1048
+NIK A L + D SDP++K+ + KT+ ++KTLDP ++E+ T +
Sbjct: 172 VNIKEARGLPAMDEQSMTSDPYIKMTILPEKKHKVKTRVLRKTLDPAFDETFTFYGIPYT 231
Query: 1049 NRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKID 1082
L I+ +D ++DD IGE ++PLS I+
Sbjct: 232 QIQELALHFTILSFDRFSRDDIIGEVLIPLSGIE 265
>Hs18543827 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 425
Score = 52.4 bits (124), Expect = 4e-06
Identities = 43/150 (28%), Positives = 70/150 (46%), Gaps = 8/150 (5%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNES--CTVQVA 1048
L + I A DL + D G SDP+VK+YL + +T+ +KTL+PT++E+ V
Sbjct: 161 LIVRILKAFDLPAKDFCGSSDPYVKIYLLPDRKCKLQTRVHRKTLNPTFDENFHFPVPYE 220
Query: 1049 NRVNNYLKIKIMDWDAGNKDDNIGEAILP--LSKIDPENPTELDIPLVLPSEKKEDAGVV 1106
+ L + + D+D ++ D IGE IL D T + + + + D G +
Sbjct: 221 ELADRKLHLSVFDFDRFSRHDMIGEVILDNLFEASDLSRETSIWKDIQYATSESVDLGEI 280
Query: 1107 HLSFEFQP---RYVLAVTR-KETKIGDLAG 1132
S + P R L V + + K D+ G
Sbjct: 281 MFSLCYLPTAGRLTLTVIKCRNLKAMDITG 310
Score = 51.2 bits (121), Expect = 9e-06
Identities = 57/247 (23%), Positives = 110/247 (44%), Gaps = 42/247 (17%)
Query: 521 DLNTGITKITIEEAKDL---DETGKAVNSYVELFV--NSKLVLTTSTIKKSEKPSW---- 571
D T + I +A DL D G + + YV++++ + K L T +K+ P++
Sbjct: 155 DYETETLIVRILKAFDLPAKDFCGSS-DPYVKIYLLPDRKCKLQTRVHRKTLNPTFDENF 213
Query: 572 --AAPYEAVVTDRRKTRIKLVVKNDKGDIISSTVQTLNDLIDRTLVAKEWIPLKNGKSSL 629
PYE + + + + + D+I + L++L + + +++E
Sbjct: 214 HFPVPYEELADRKLHLSVFDFDRFSRHDMIGEVI--LDNLFEASDLSRE----------- 260
Query: 630 KISTQWKPV------SLDIGSN--DVAYTPPVGVLRILLNKATGLKNLEKFGTIDPYARV 681
++ WK + S+D+G + Y P G L + + K LK ++ G DPY +V
Sbjct: 261 --TSIWKDIQYATSESVDLGEIMFSLCYLPTAGRLTLTVIKCRNLKAMDITGYSDPYVKV 318
Query: 682 LV----NNLPKGRTNVVESTVNPVWNEAIYVAVSSSNQ---KVSIECLDVEYAGEDRSLG 734
+ L K +T + ++T+NPV+NEAI + N + I +D + G + +G
Sbjct: 319 SLLCDGRRLKKKKTTIKKNTLNPVYNEAIIFDIPPENMDQVSLLISVMDYDRVGHNEIIG 378
Query: 735 KVDIPIS 741
+ I+
Sbjct: 379 VCRVGIT 385
>CE29448 [TU] KOG1028 Ca2+-dependent phospholipid-binding protein
Synaptotagmin required for synaptic vesicle and secretory
granule exocytosis
Length = 458
Score = 52.4 bits (124), Expect = 4e-06
Identities = 33/104 (31%), Positives = 51/104 (48%), Gaps = 4/104 (3%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPF--YKTKTIKKTLDPTWNESCTVQVA 1048
L IN+ A +L D G SDP+VK+ NG F K+ T + L+P ++E+ +
Sbjct: 316 LYINVSKAYNLRPMDITGASDPYVKIEQTFNGKRFKSKKSSTKRANLNPVFHETLVFDIP 375
Query: 1049 NR--VNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPENPTELD 1090
+ + I++MDWD KDD +G IL + T+ D
Sbjct: 376 SSQIAETNILIQVMDWDRIGKDDLLGCCILGKDSPTADGKTQWD 419
>Hs4504797_2 [T] KOG4305 RhoGEF GTPase
Length = 500
Score = 51.6 bits (122), Expect = 7e-06
Identities = 30/103 (29%), Positives = 53/103 (51%), Gaps = 3/103 (2%)
Query: 979 LPQSDLITNCGDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPT 1038
L +S T G L +N+ +L +GKS+P+ ++ + GS + TKTI+ TL+P
Sbjct: 365 LVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTM---GSQCHITKTIQDTLNPK 421
Query: 1039 WNESCTVQVANRVNNYLKIKIMDWDAGNKDDNIGEAILPLSKI 1081
WN +C + + L I + + D + DD +G + ++ I
Sbjct: 422 WNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADI 464
>At4g05330_2 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 165
Score = 51.6 bits (122), Expect = 7e-06
Identities = 28/99 (28%), Positives = 53/99 (53%), Gaps = 3/99 (3%)
Query: 375 VSGSALSIGVLELTVKNAKDLKRSNLMNISVDPYLQFSIGGRVLGKTRTVKDTLNPVWNE 434
+ G IG+L++T+K +L ++M S DPY+ ++G + L +T + LNPVWN+
Sbjct: 1 MEGMVEFIGLLKVTIKKGTNLAIRDMM--SSDPYVVLNLGKQKL-QTTVMNSNLNPVWNQ 57
Query: 435 SMFILLASFTDPLEITVYDKREHLKDKVLGRIYYNLSSL 473
+ + + P+++ VYD D ++G ++ L
Sbjct: 58 ELMLSVPESYGPVKLQVYDYDTFSADDIMGEADIDIQPL 96
>Hs4758856 [M] KOG1326 Membrane-associated protein FER-1 and related ferlins
contain multiple C2 domains
Length = 1230
Score = 51.2 bits (121), Expect = 9e-06
Identities = 30/95 (31%), Positives = 50/95 (52%), Gaps = 6/95 (6%)
Query: 991 LKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPFYKTKTIKKTLDPTWNESCTVQVANR 1050
+++ + A DL +D NGK+DP++ + L K I K L+P + +S ++ +
Sbjct: 727 VRVYVVRATDLHPADINGKADPYIAIRLGKTDIRD-KENYISKQLNPVFGKSFDIEASFP 785
Query: 1051 VNNYLKIKIMDWDAGNKDDNIGEAILPLSKIDPEN 1085
+ + L + + DWD DD IGE +KID EN
Sbjct: 786 MESMLTVAVYDWDLVGTDDLIGE-----TKIDLEN 815
>Hs20553661 [T] KOG1327 Copine
Length = 298
Score = 51.2 bits (121), Expect = 9e-06
Identities = 30/92 (32%), Positives = 43/92 (46%), Gaps = 6/92 (6%)
Query: 988 CGDLKINIKSANDLISSDRNGKSDPFVKLYLNDNGSPF---YKTKTIKKTLDPTWNE--- 1041
C D + AN L D GKSDPF+ Y ++ F +KT+ +K TL+P W
Sbjct: 156 CRDAVLMQFCANKLDKKDFFGKSDPFLVFYRSNEDGSFTICHKTEVVKNTLNPVWQAFKI 215
Query: 1042 SCTVQVANRVNNYLKIKIMDWDAGNKDDNIGE 1073
S + +K+++ DWD D IGE
Sbjct: 216 SVRALCNGDYDRTIKVEVYDWDRDGSHDFIGE 247
>At1g70800 [R] KOG1030 Predicted Ca2+-dependent phospholipid-binding protein
Length = 174
Score = 51.2 bits (121), Expect = 9e-06
Identities = 27/83 (32%), Positives = 48/83 (57%), Gaps = 3/83 (3%)
Query: 382 IGVLELTVKNAKDLKRSNLMNISVDPYLQFSIGGRVLGKTRTVKDTLNPVWNESMFILLA 441
+G++ + VK DL R + + S DP++ ++G + L K+ TVK+ NP WNE + + +
Sbjct: 14 VGLVRILVKRGIDLARRDAL--SSDPFVVITMGPQKL-KSFTVKNNCNPEWNEELTLAIE 70
Query: 442 SFTDPLEITVYDKREHLKDKVLG 464
+P+++ VYDK D +G
Sbjct: 71 DPNEPVKLMVYDKDTFTADDKMG 93
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.135 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 66,483,746
Number of Sequences: 60738
Number of extensions: 2887547
Number of successful extensions: 8314
Number of sequences better than 1.0e-05: 68
Number of HSP's better than 0.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 55
Number of HSP's that attempted gapping in prelim test: 7870
Number of HSP's gapped (non-prelim): 286
length of query: 1173
length of database: 30,389,216
effective HSP length: 118
effective length of query: 1055
effective length of database: 23,222,132
effective search space: 24499349260
effective search space used: 24499349260
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)