ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3400 good O KOG2603 Posttranslational modification, protein turnover, chaperones Oligosaccharyltransferase, gamma subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3400 1195072 1194038 -345
(345 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR085w [O] KOG2603 Oligosaccharyltransferase gamma subunit 177 2e-44
7297376 [O] KOG2603 Oligosaccharyltransferase gamma subunit 87 3e-17
CE00457 [O] KOG2603 Oligosaccharyltransferase gamma subunit 81 2e-15
Hs6996934 [O] KOG2603 Oligosaccharyltransferase gamma subunit 79 1e-14
Hs14149775 [O] KOG2603 Oligosaccharyltransferase gamma subunit 73 7e-13
>YOR085w [O] KOG2603 Oligosaccharyltransferase gamma subunit
Length = 350
Score = 177 bits (449), Expect = 2e-44
Identities = 105/354 (29%), Positives = 186/354 (51%), Gaps = 15/354 (4%)
Query: 3 LSTVFATFLVWYMSLVT----AISNKRLLELSQKDGN-VIKLNSKNYEKILNSPRKSD-I 56
++ +F LV++ + T A+S+ RLL+L+ K +I L ++E IL P ++ I
Sbjct: 1 MNWLFLVSLVFFCGVSTHPALAMSSNRLLKLANKSPKKIIPLKDSSFENILAPPHENAYI 60
Query: 57 VVFFTATATQFSCTLCLEMSPSFDVVANSWFSDHANGISKELENHGLFFAKSDFNAESKQ 116
V FTATA + C+LCLE+ +D + SWF DH + S + +FF K + SK
Sbjct: 61 VALFTATAPEIGCSLCLELESEYDTIVASWFDDHPDAKSSNSDT-SIFFTKVNLEDPSKT 119
Query: 117 L---FSQFQLTSVPAFLVFKAGGKSINDVEKITVATELGANHLNFLADNIKNAVQIPDLF 173
+ F FQL +VP +FK SI D I+++T+ G+ + + IK Q+ D
Sbjct: 120 IPKAFQFFQLNNVPRLFIFKPNSPSILDHSVISISTDTGSERMKQIIQAIKQFSQVNDFS 179
Query: 174 VYEPINWGACIXXXXXXXXXXXXXXRYTSALLNVLTLRPLWGIACSFCITTLIAGAMFIK 233
++ P++W I + + + ++++ R +W +F I +I+ MF +
Sbjct: 180 LHLPMDWTPIITSTIITFITVLLFKKQSKLMFSIISSRIIWATLSTFFIICMISAYMFNQ 239
Query: 234 IRGSPFSGMSADRKSIVYFLEGQLQNQYAIESQIITVLYSVLASSFIGLICVVPYIQKWY 293
IR + +G+ + ++YFL + Q+Q+AIE+Q++ ++Y LA+ + L+ + +++
Sbjct: 240 IRNTQLAGV-GPKGEVMYFLPNEFQHQFAIETQVMVLIYGTLAALVVVLVKGIQFLRSHL 298
Query: 294 QKKQHYGKAAIVNFSLALFFTAVIYIFYSALMAVFALKNTGYPFKLFK--NPFK 345
+ KA ++ LA F IY+F++AL VF +K+ YPF L + PFK
Sbjct: 299 YPETK--KAYFIDAILASFCALFIYVFFAALTTVFTIKSPAYPFPLLRLSAPFK 350
>7297376 [O] KOG2603 Oligosaccharyltransferase gamma subunit
Length = 347
Score = 87.4 bits (215), Expect = 3e-17
Identities = 83/329 (25%), Positives = 141/329 (42%), Gaps = 45/329 (13%)
Query: 23 NKRLLELSQKDGNVIKLNSKN----------YEKILNSPRKSDIVVFFTATATQFSCTLC 72
+K L LS+K N++ +N+K E + ++PR ++V TA A C +C
Sbjct: 28 SKTGLSLSEKVQNLVDMNAKKPLLRFNGPKFREYVKSAPRNYSMIVMLTALAPSRQCQIC 87
Query: 73 LEMSPSFDVVANSW-FSDHANGISKELENHGLFFAKSDFNAESKQLFSQFQLTSVPAFLV 131
F +VANS+ FS ++ LFFA DF+ + ++F +L + P F+
Sbjct: 88 RHAHDEFAIVANSYRFS--------STYSNKLFFAMVDFD-DGSEVFQLLRLNTAPVFMH 138
Query: 132 FKAGGKSINDVEKITVATELGANHLNFLADNI-KNAVQIPDLF--VYEPINWGACIXXXX 188
F A GK A + + + F AD+I K + D+ ++ P N+ +
Sbjct: 139 FPAKGKPKG-------ADTMDIHRVGFAADSIAKFVAERTDITIRIFRPPNYSGTVAMIT 191
Query: 189 XXXXXXXXXXRYTSALLNVLTLRPLWGIACSFCITTLIAGAMFIKIRGSPFSGMSADRKS 248
+ L L + LWG F +I+G M+ IRG P + +
Sbjct: 192 LVALVGSFLYIRRNN-LEFLYNKNLWGAIAVFFCFAMISGQMWNHIRGPPL--VHKSQNG 248
Query: 249 IVYFLEGQLQNQYAIESQIITVLYSVLASSFIGLICVVPYIQKWYQKKQHYGKAAIVNFS 308
V ++ G Q Q +E+ I+ L +++ I LI + KA N
Sbjct: 249 GVAYIHGSSQGQLVVETYIVMFLNAMIVLGMILLI------------ESGTPKAHNKNRI 296
Query: 309 LALFFTAVIYIFYSALMAVFALKNTGYPF 337
+A+ ++ +F+S L++VF K GYP+
Sbjct: 297 MAMTGLVLLTVFFSFLLSVFRSKAQGYPY 325
>CE00457 [O] KOG2603 Oligosaccharyltransferase gamma subunit
Length = 331
Score = 81.3 bits (199), Expect = 2e-15
Identities = 85/329 (25%), Positives = 148/329 (44%), Gaps = 36/329 (10%)
Query: 24 KRLLELSQKDGNVIKLNSKNYEKILN-SPRKSDIVVFFTATATQFSCTLCLEMSPSFDVV 82
+ L++L+ + +++K N ++ ++ PR ++V FTA + C +C F +V
Sbjct: 19 QNLVDLTSRQ-SIVKFNMDKWKTLVRMQPRNYSMIVMFTALSPGVQCPICKPAYDEFMIV 77
Query: 83 ANSWFSDHANGISKELENHGLFFAKSDFNAESKQLFSQFQLTSVPAFLVF--KAGGK--- 137
ANS S E + +FF D+ ++ Q+F Q L + P F K G K
Sbjct: 78 ANS-----HRYTSSEGDRRKVFFGIVDYE-DAPQIFQQMNLNTAPILYHFGPKLGAKKRP 131
Query: 138 -SINDVEKITVATELGANHLNFLADNIKNAVQIPDLFVYEPINWGACIXXXXXXXXXXXX 196
++ + A +G F+AD + V+ V P N+ A +
Sbjct: 132 EQMDFQRQGFDADAIG----RFVADQTEVHVR-----VIRPPNYTAPVVIALFVALLLGM 182
Query: 197 XXRYTSALLNVLTLRPLWGIACSFCITTLIAGAMFIKIRGSPFSGMSADRKSIVYFLEGQ 256
++ L+ L R +WG C ++G M+ IRG PF + + K F+ G
Sbjct: 183 LYMKRNS-LDFLFNRTVWGFVCLAITFIFMSGQMWNHIRGPPFMITNPNTKE-PSFIHGS 240
Query: 257 LQNQYAIESQIITVLYSVLASSFIGLICVVPYIQKWYQK-KQHYGK----AAIVNF---S 308
Q Q E+ I+ +LY+++A IG ICV + K +++ GK +++N +
Sbjct: 241 TQFQLIAETYIVGLLYALIA---IGFICVNEAADQSNSKDRKNAGKKLNPLSLLNIPTNT 297
Query: 309 LALFFTAVIYIFYSALMAVFALKNTGYPF 337
LA+ I +F+S L++VF K GYP+
Sbjct: 298 LAIAGLVCICVFFSFLLSVFRSKYRGYPY 326
>Hs6996934 [O] KOG2603 Oligosaccharyltransferase gamma subunit
Length = 348
Score = 78.6 bits (192), Expect = 1e-14
Identities = 72/319 (22%), Positives = 135/319 (41%), Gaps = 35/319 (10%)
Query: 24 KRLLELSQKDGNVIKLNSKNYEKILNSP-RKSDIVVFFTATATQFSCTLCLEMSPSFDVV 82
++L+E S + ++ ++N + K + +P R ++V FTA Q C++C + + + ++
Sbjct: 54 EQLMEWSSRR-SIFRMNGDKFRKFIKAPPRNYSMIVMFTALQPQRQCSVCRQANEEYQIL 112
Query: 83 ANSWFSDHANGISKELENHGLFFAKSDFNAESKQLFSQFQLTSVPAFLVFKAGGK----S 138
ANSW A + LFF+ D++ E +F Q + S P F G+
Sbjct: 113 ANSWRYSSA-------FCNKLFFSMVDYD-EGTDVFQQLNMNSAPTFXHXPPKGRPKRAD 164
Query: 139 INDVEKITVATELGANHLNFLADNIKNAVQIPDLFVYEPINWGACIXXXXXXXXXXXXXX 198
D+++I A E A ++AD ++ V+ P N+ I
Sbjct: 165 TFDLQRIGFAAEQLA---KWIADRTDVHIR-----VFRPPNYSGTIALALLVSLVGGLLY 216
Query: 199 RYTSALLNVLTLRPLWGIACSFCITTLIAGAMFIKIRGSPFSGMSADRKSIVYFLEGQLQ 258
+ L + + W + + + +G M+ IRG P++ + + Y + G Q
Sbjct: 217 XRRNN-LEFIYNKTGWAMVSLCIVFAMTSGQMWNHIRGPPYAHKNPHNGQVSY-IHGSSQ 274
Query: 259 NQYAIESQIITVLYSVLASSFIGLICVVPYIQKWYQKKQHYGKAAIVNFSLALFFTAVIY 318
Q+ ES II VL + + + + + K GK I+ L ++
Sbjct: 275 AQFVAESHIILVLNAAITMGMV-------LLNEAATSKGDVGKRRII----CLVGLGLVV 323
Query: 319 IFYSALMAVFALKNTGYPF 337
F+S L+++F K GYP+
Sbjct: 324 FFFSFLLSIFRSKYHGYPY 342
>Hs14149775 [O] KOG2603 Oligosaccharyltransferase gamma subunit
Length = 335
Score = 72.8 bits (177), Expect = 7e-13
Identities = 65/304 (21%), Positives = 124/304 (40%), Gaps = 28/304 (9%)
Query: 36 VIKLNSKNYEKILNSP-RKSDIVVFFTATATQFSCTLCLEMSPSFDVVANSWFSDHANGI 94
VI++N + +++ +P R ++V FTA C +C + F ++ANSW A
Sbjct: 53 VIRMNGDKFRRLVKAPPRNYSVIVMFTALQLHRQCVVCKQADEEFQILANSWRYSSAF-- 110
Query: 95 SKELENHGLFFAKSDFNAESKQLFSQFQLTSVPAFLVFKAGGK-SINDVEKITVATELGA 153
+ +FFA DF+ E +F + S P F+ F A GK D ++ V
Sbjct: 111 -----TNRIFFAMVDFD-EGSDVFQMLNMNSAPTFINFPAKGKPKRGDTYELQVRGFSAE 164
Query: 154 NHLNFLADNIKNAVQIPDLFVYEPINWGACIXXXXXXXXXXXXXXRYTSALLNVLTLRPL 213
++AD +++ + P G + R ++ + L +
Sbjct: 165 QIARWIADRTDVNIRV----IRPPNYAGPLMLGLLLAVIGGLVYLRRSN--MEFLFNKTG 218
Query: 214 WGIACSFCITTLIAGAMFIKIRGSPFSGMSADRKSIVYFLEGQLQNQYAIESQIITVLYS 273
W A + + +G M+ IRG P++ + + Y + G Q Q+ E+ I+ +
Sbjct: 219 WAFAALCFVLAMTSGQMWNHIRGPPYAHKNPHTGHVNY-IHGSSQAQFVAETHIVLLFNG 277
Query: 274 VLASSFIGLICVVPYIQKWYQKKQHYGKAAIVNFSLALFFTAVIYIFYSALMAVFALKNT 333
+ + L+C K++ A I ++ +F+S ++++F K
Sbjct: 278 GVTLGMV-LLCEAATSDMDIGKRKIMCVAGI----------GLVVLFFSWMLSIFRSKYH 326
Query: 334 GYPF 337
GYP+
Sbjct: 327 GYPY 330
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.325 0.137 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,969,893
Number of Sequences: 60738
Number of extensions: 665124
Number of successful extensions: 2188
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2175
Number of HSP's gapped (non-prelim): 6
length of query: 345
length of database: 30,389,216
effective HSP length: 107
effective length of query: 238
effective length of database: 23,890,250
effective search space: 5685879500
effective search space used: 5685879500
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)