ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3425 good R KOG2140 General function prediction only Uncharacterized conserved protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3425 1206080 1207666 529
(529 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR278w [R] KOG2140 Uncharacterized conserved protein 245 1e-64
SPBC13E7.01 [R] KOG2140 Uncharacterized conserved protein 207 3e-53
At1g80930 [R] KOG2140 Uncharacterized conserved protein 197 4e-50
Hs20536216 [R] KOG2140 Uncharacterized conserved protein 196 6e-50
CE17753 [R] KOG2140 Uncharacterized conserved protein 186 8e-47
Hs22046307 [T] KOG2141 Protein involved in high osmolarity signa... 62 2e-09
>YGR278w [R] KOG2140 Uncharacterized conserved protein
Length = 577
Score = 245 bits (625), Expect = 1e-64
Identities = 157/480 (32%), Positives = 253/480 (52%), Gaps = 26/480 (5%)
Query: 18 LSHVLTNIDSANILGNLRDLIEVNILRHRRLFVATLLSLQCENDKDS---AFAGLVKLID 74
+S +++N+ N+ + RDL +VNIL R + ++ A + L+ L++
Sbjct: 25 VSPIISNLTMDNLQESHRDLFQVNILIGRNIICKNVVDFTLNKQNGRLIPALSALIALLN 84
Query: 75 HYLPSIGFLTGRECVLRLIDAVYRSDKKVYFNMASLLSFLIIDEVIDEGTAFALIYFLLR 134
+P IG +E +L + R D N+ LS L + +VI E ++ LL
Sbjct: 85 SDIPDIGETLAKELMLMFVQQFNRKDYVSCGNILQCLSILFLYDVIHEIVILQILLLLLE 144
Query: 135 NKTDDNXXXXXXXXXXXGQQLQAFDKESEADIAEKLRLIYQDRYTQKHTYKALNRLFDTR 194
++ G +L K++ I EKLR I Q + ++L LF+ R
Sbjct: 145 K---NSLRLVIAVMKICGWKLALVSKKTHDMIWEKLRYILQTQELSSTLRESLETLFEIR 201
Query: 195 RRSYRNVIKNVNI--PTVEN-NVHEVVVDFSNDHPAMDLDGFHLDDKYELINEQYXXXXX 251
++ Y++ + + I PT + H +V D +L F + + + +
Sbjct: 202 QKDYKSGSQGLFILDPTSYTVHTHSYIVS-DEDEANKELGNFEKCENFNELTMAFDTLRQ 260
Query: 252 XXXXXXXXXXDVKNS----LNDMTDSENTEFKKKVYLILKGSLSGDEAAHKLLQLRLNDA 307
+ ++ + DMT + + EFKKK+YL+LK SLSGDEAAHKLL+L++ +
Sbjct: 261 KLLINNTSDTNEGSNSQLQIYDMTSTNDVEFKKKIYLVLKSSLSGDEAAHKLLKLKIANN 320
Query: 308 KKNEVADTITKACAQEPTYSKFYGILTERLTSFHVSWQHSFSAVFKENY-QTIADNDPSN 366
K V D I K+ QE T+SKFY IL+ER+ +FH SWQ +++ F++NY Q I D +
Sbjct: 321 LKKSVVDIIIKSSLQESTFSKFYSILSERMITFHRSWQTAYNETFEQNYTQDIEDYETDQ 380
Query: 367 IRNLGKFWGHALATECIPFEVFEIVHMNERDSNAANRVFLKFLFQEMVVNLGIDALKKKL 426
+R LGKFWGH ++ E +P + +I+ + E +S R+F+KFLFQE+V LG+D L+ +L
Sbjct: 381 LRILGKFWGHLISYEFLPMDCLKIIKLTEEESCPQGRIFIKFLFQELVNELGLDELQLRL 440
Query: 427 EAVELQPFLSNLFPREAQ-DDIMFSINYFTAIGLGALTDNMRNIL------QQAEEEQRK 479
+ + L +FP E + I +SIN+FTAIGLG LT++MR+ L + AEEE++K
Sbjct: 441 NSSK----LDGMFPLEGDAEHIRYSINFFTAIGLGLLTEDMRSRLTIIQEVEDAEEEEKK 496
>SPBC13E7.01 [R] KOG2140 Uncharacterized conserved protein
Length = 628
Score = 207 bits (527), Expect = 3e-53
Identities = 127/496 (25%), Positives = 242/496 (48%), Gaps = 30/496 (6%)
Query: 18 LSHVLTNIDSANILGNLRDLIEVNILRHRRLFVATLLSLQCEN-DKDSAFAGLVKLIDHY 76
++ ++ ++ +NI + +L + NI+R R L+ +++ Q + +A + +I+
Sbjct: 127 INGLINKVNKSNIRDIIPELFQENIIRGRALYCRSIMKAQAASLPFTPIYAAMTAVINTK 186
Query: 77 LPSIGFLTGRECVLRLIDAVYRSDKKVYFNMASLLSFLIIDEVIDEGTAFALIYFLLRNK 136
P IG L +++ + R+DK + + +S ++ LI ++ E ++ LL
Sbjct: 187 FPQIGELLLTRLIVQFRKSFQRNDKSMCISSSSFIAHLINQKIAHEIVGLQILAVLLERP 246
Query: 137 TDDNXXXXXXXXXXXGQQLQAFDKESEADIAEKLRLIYQDRYTQKHTYKALNRLFDTRRR 196
T+D+ G L + + E+ R I + ++ T + LF TR+
Sbjct: 247 TNDSIEIAVMLLREIGAYLAEVSTRAYNGVFERFRTILHEGQLERRTQFIIEVLFQTRKD 306
Query: 197 SYRN---VIKNVNIPTVENNVHEVVVDFSNDHPAMDLDGFHLDDKYELINEQYXXXX--- 250
++N + + +++ E+ + + N L FH D YE ++Y
Sbjct: 307 KFKNNPTIPQELDLVEEEDQITHYISLDDNLDVQESLGIFHYDPDYEENEKKYDAIKHEI 366
Query: 251 ------------------XXXXXXXXXXXDVKNSLNDMTDSENTEFKKKVYLILKGSLSG 292
D K + D T++ +K +YL + S+
Sbjct: 367 LGEEDDDENEEDEEDSEETSESEEDESVNDEKPQVIDQTNASLVNLRKSIYLTIMSSVDF 426
Query: 293 DEAAHKLLQLRLNDAKKNEVADTITKACAQEPTYSKFYGILTERLTSFHVSWQHSFSAVF 352
+E HKLL++ L + ++ E+ + + + +QE TY+KFYG++ ER +W+ ++ F
Sbjct: 427 EECCHKLLKIDLPEGQEIELCNMVIECNSQERTYAKFYGLIGERFCKLSRTWRSTYEQCF 486
Query: 353 KENYQTIADNDPSNIRNLGKFWGHALATECIPFEVFEIVHMNERDSNAANRVFLKFLFQE 412
K Y+TI + + +RN+ F+ + L+T+ I +EV++ V + E D+ A++R+FLK +FQE
Sbjct: 487 KNYYETIHRYETNRLRNIALFFANLLSTDSIGWEVYDCVRLTEDDTTASSRIFLKIMFQE 546
Query: 413 MVVNLGIDALKKKLEAVELQPFLSNLFPREAQDDIMFSINYFTAIGLGALTDNMRNIL-- 470
+V LG+ +L ++L L P+L LFP + ++ FSINYFT+IGLGALT+ MR L
Sbjct: 547 IVEALGLKSLVERLHDPNLVPYLHGLFPVDEARNVRFSINYFTSIGLGALTEEMREYLLT 606
Query: 471 ---QQAEEEQRKRYSN 483
+ +E+ + YS+
Sbjct: 607 MPKSEPKEQDSEGYSS 622
>At1g80930 [R] KOG2140 Uncharacterized conserved protein
Length = 900
Score = 197 bits (500), Expect = 4e-50
Identities = 129/513 (25%), Positives = 242/513 (47%), Gaps = 33/513 (6%)
Query: 15 RLILSHVLTNIDSANILGNLRDLIEVNILRHRRLFVATLLSLQCENDK-DSAFAGLVKLI 73
R ++ ++ ++++NI + +L N++R R LF + + Q + FA LV +I
Sbjct: 363 RKSINGLVNKVNASNIKNIIPELFAENLIRGRGLFCRSCMKSQMASPGFTDVFAALVAVI 422
Query: 74 DHYLPSIGFLTGRECVLRLIDAVYRSDKKVYFNMASLLSFLIIDEVIDEGTAFALIYFLL 133
+ P + L + VL+L A R+DK ++ L+ +V +E A L+ LL
Sbjct: 423 NAKFPEVAELLLKRVVLQLKRAYKRNDKPQLLAAVKFIAHLVNQQVAEEIIALELVTILL 482
Query: 134 RNKTDDNXXXXXXXXXXXGQQLQAFDKESEADIAEKLRLIYQDRYTQKHTYKALNRLFDT 193
+ TDD+ G LQ I E+ R I + K + LF T
Sbjct: 483 GDPTDDSVEVAVGFVTECGAMLQDVSPRGLNGIFERFRGILHEGEIDKRVQYLIESLFAT 542
Query: 194 RRRSYRN--VIKNVNIPTVENNVHEVVVDFSNDHPAMDLDGFHLDD-------KYELINE 244
R+ ++ ++ E H++ +D D P LD F D KYE + +
Sbjct: 543 RKAKFQGHPAVRPELDLVEEKYSHDLSLDHEID-PETALDIFKPDPDFVENEKKYEALKK 601
Query: 245 QYXXXXXXXXXXXXXXXDVKNS---------------LNDMTDSENTEFKKKVYLILKGS 289
+ N + D T++ ++ +YL + S
Sbjct: 602 ELLGDEESEDEDGSDASSEDNDEEEDESDEEDEEQMRIRDETETNLVNLRRTIYLTIMSS 661
Query: 290 LSGDEAAHKLLQLRLNDAKKNEVADTITKACAQEPTYSKFYGILTERLTSFHVSWQHSFS 349
+ +EA HKLL+++L ++ E+ + + C+QE TY ++YG+L +R + Q +F
Sbjct: 662 VDFEEAGHKLLKIKLEPGQEMELCIMLLECCSQERTYLRYYGLLGQRFCMINKIHQENFE 721
Query: 350 AVFKENYQTIADNDPSNIRNLGKFWGHALATECIPFEVFEIVHMNERDSNAANRVFLKFL 409
F + Y I + + +RN+ KF+ H L T+ +P+ V + + E D+ +++R+F+K L
Sbjct: 722 KCFVQQYSMIHRLETNKLRNVAKFFAHLLGTDALPWHVLAYIRLTEEDTTSSSRIFIKIL 781
Query: 410 FQEMVVNLGIDALKKKLEAVELQPFLSNLFPREAQDDIMFSINYFTAIGLGALTDNMR-- 467
FQE+ +LGI L ++L+ +Q L ++FP++ + F+IN+FT+IGLG +T+N+R
Sbjct: 782 FQELSEHLGIRLLNERLQDPTMQESLESIFPKDNPKNTRFAINFFTSIGLGGITENLREY 841
Query: 468 -----NILQQAEEEQRKRYSNAIKNSEVSERDN 495
+++ Q +++ + S + S+ S+ ++
Sbjct: 842 LKNMPSLIMQRQKQVAESESGSSSGSDSSDSES 874
>Hs20536216 [R] KOG2140 Uncharacterized conserved protein
Length = 908
Score = 196 bits (499), Expect = 6e-50
Identities = 129/517 (24%), Positives = 242/517 (45%), Gaps = 39/517 (7%)
Query: 18 LSHVLTNIDSANILGNLRDLIEVNILRHRRLFVATLLSLQCENDKDS-AFAGLVKLIDHY 76
++ ++ ++ +NI +++L++ NI+R R L ++L Q + + +A LV +I+
Sbjct: 166 INGLINKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSK 225
Query: 77 LPSIGFLTGRECVLRLIDAVYRSDKKVYFNMASLLSFLIIDEVIDEGTAFALIYFLLRNK 136
P IG L + +L R+DK++ + ++ LI V E ++ LL
Sbjct: 226 FPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERP 285
Query: 137 TDDNXXXXXXXXXXXGQQLQAFDKESEADIAEKLRLIYQDRYTQKHTYKALNRLFDTRRR 196
TDD+ G +L I E+LR I + K + +F R+
Sbjct: 286 TDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKD 345
Query: 197 SYRN---VIKNVNIPTVENNVHEVVVDFSNDHPAMDLDGFHLDDKYELINEQYXXXXXXX 253
+++ +++ +++ ++ ++ + +P L+ F +D + E+Y
Sbjct: 346 GFKDHPIILEGLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEI 405
Query: 254 XXXXXXXXDV-------------------------KNSLNDMTDSENTEFKKKVYLILKG 288
+ K +++D T+ F++ +YL ++
Sbjct: 406 LDEGDTDSNTDQDAGSSEEDEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQS 465
Query: 289 SLSGDEAAHKLLQLRLNDAKKNEVADTITKACAQEPTYSKFYGILTERLTSFHVSWQHSF 348
SL +E AHKLL++ +++ E+ + I CAQ+ TY KF+G+L R + SF
Sbjct: 466 SLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESF 525
Query: 349 SAVFKENYQTIADNDPSNIRNLGKFWGHALATECIPFEVFEIVHMNERDSNAANRVFLKF 408
+FKE Y TI + + +RN+ K + H L T+ +P+ V E + ++E + +++R+F+K
Sbjct: 526 EGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKI 585
Query: 409 LFQEMVVNLGIDALKKKLEAVELQPFLSNLFPREAQDDIMFSINYFTAIGLGALTDNMRN 468
FQE+ +G+ L +L+ LQPF L PR+ + F+IN+FT+IGLG LTD +R
Sbjct: 586 FFQELCEYMGLPKLNARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELRE 645
Query: 469 ---------ILQQAEEEQRKRY-SNAIKNSEVSERDN 495
+ Q+ + EQ K S++ S SE D+
Sbjct: 646 HLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDS 682
>CE17753 [R] KOG2140 Uncharacterized conserved protein
Length = 897
Score = 186 bits (472), Expect = 8e-47
Identities = 126/490 (25%), Positives = 241/490 (48%), Gaps = 32/490 (6%)
Query: 20 HVLTN-IDSANILGNLRDLIEVNILRHRRLFVATLLSLQCENDKDS-AFAGLVKLIDHYL 77
H L N +++ N++ +R+L++ N++R + L ++ Q + S +A L +I+
Sbjct: 198 HGLVNRVNAKNLVQIVRELLQENVIRSKGLLCRDIIQAQAFSPGFSNVYAALAAVINSKF 257
Query: 78 PSIGFLTGRECVLRLIDAVYRSDKKVYFNMASLLSFLIIDEVIDEGTAFALIYFLLRNKT 137
P +G L R +++ + R+D+ V N+ ++ LI +V E A ++ +L T
Sbjct: 258 PHVGELLLRRLIVQFKRSFRRNDRGVTVNVIKFIAHLINQQVAHEVLALEIMILMLEEPT 317
Query: 138 DDNXXXXXXXXXXXGQQLQAFDKESEADIAEKLRLIYQDRYTQKHTY-KALNRLFDTRRR 196
DD+ G +L + + ++LR I + ++ + + + +T +
Sbjct: 318 DDSVEVAIAFLKECGAKLLEIAPAALNSVYDRLRAILMETERSENALDRRIQYMIETAMQ 377
Query: 197 -------SYRNVIKNVNIPTVENNV-HEVVVDFSNDHPAMDLDGFHLDDKYELINEQYXX 248
+Y VI+++++ E+ + H + ++ + D P L+ F LD ++E E Y
Sbjct: 378 IRKDKFAAYPAVIEDLDLIEEEDQIIHTLNLEDAVD-PENGLNVFKLDPEFEKNEEVYEE 436
Query: 249 XXXXXXXXXXXX--------------XDVKNS------LNDMTDSENTEFKKKVYLILKG 288
DV+ + + D TD T F+++VYL ++
Sbjct: 437 IRKEIIGNADISDEDGGDELDDEEEGSDVEEAPKKTTEIIDNTDQNLTAFRREVYLTMQS 496
Query: 289 SLSGDEAAHKLLQLRLNDAKKNEVADTITKACAQEPTYSKFYGILTERLTSFHVSWQHSF 348
SL EAAHKLL++++ D+ +NE+ + CAQ+ TY +FYG+L ER + +Q F
Sbjct: 497 SLDYQEAAHKLLKMKIPDSMQNELCAMLVDCCAQQRTYERFYGMLIERFCRLRLEYQQYF 556
Query: 349 SAVFKENYQTIADNDPSNIRNLGKFWGHALATECIPFEVFEIVHMNERDSNAANRVFLKF 408
+ ++ Y TI D + +RNL + H L+T+ I +++ + M E D+ ++ R+++K+
Sbjct: 557 EKLCQDTYSTIHRIDITKLRNLARLIAHLLSTDAIDWKILADMKMTEEDTTSSGRIYIKY 616
Query: 409 LFQEMVVNLGIDALKKKLEAVELQPFLSNLFPREAQDDIMFSINYFTAIGLGALTDNMRN 468
+F E+V +G+ L ++ L LFPR + FSIN+FT IGLG LT +R
Sbjct: 617 IFNELVEAMGMVKLHSRVTDPTLAHCFVGLFPRTNPNSARFSINFFTMIGLGGLTLELRE 676
Query: 469 ILQQAEEEQR 478
L + ++++
Sbjct: 677 WLAKGLKKKK 686
>Hs22046307 [T] KOG2141 Protein involved in high osmolarity signaling pathway
Length = 356
Score = 62.4 bits (150), Expect = 2e-09
Identities = 37/164 (22%), Positives = 73/164 (43%), Gaps = 2/164 (1%)
Query: 275 NTEFKKKVYLILKGSLSGDEAAHKLLQLRLNDAKKNEVADTITKACAQEPTYSKFYGILT 334
NT+ ++ ++ + S +A KLL+L L D ++ E+ + C QE TY+ FY L
Sbjct: 148 NTDIRRNIFCTIMTSEDFLDAFEKLLKLGLKDQQEREIIHVLMDCCLQEKTYNPFYAFLA 207
Query: 335 ERLTSFHVSWQHSFSAVFKENYQTIADNDPSNIRNLGKFWGHALATECIPFEVFEIVHMN 394
+ + +Q +F + ++ + + +N NL H L T+ + + ++V +
Sbjct: 208 SKFCEYERRFQMTFQFSIWDKFRDLENLPATNFSNLVHLVAHLLKTKSLSLSILKVVEFS 267
Query: 395 ERDSNAANRVFLKFLFQEMVVNLGIDALKKKLEAVELQPFLSNL 438
E D FL+ + +++ ++ L V P L L
Sbjct: 268 ELDKPRVR--FLRKVLSILLMETEVEDLSLIFTRVSDNPKLGVL 309
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.136 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,090,760
Number of Sequences: 60738
Number of extensions: 1042154
Number of successful extensions: 2762
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2744
Number of HSP's gapped (non-prelim): 7
length of query: 529
length of database: 30,389,216
effective HSP length: 111
effective length of query: 418
effective length of database: 23,647,298
effective search space: 9884570564
effective search space used: 9884570564
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)