ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3616 good J KOG2963 Translation, ribosomal structure and biogenesis RNA-binding protein required for 60S ribosomal subunit biogenesis
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3616 1275944 1274523 -474
(474 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR312w [J] KOG2963 RNA-binding protein required for 60S ribosom... 483 e-136
YHR066w [J] KOG2963 RNA-binding protein required for 60S ribosom... 482 e-136
SPAC6B12.01 [J] KOG2963 RNA-binding protein required for 60S rib... 204 3e-52
Hs9910566 [J] KOG2963 RNA-binding protein required for 60S ribos... 168 1e-41
7300704 [J] KOG2963 RNA-binding protein required for 60S ribosom... 157 3e-38
At5g61770 [J] KOG2963 RNA-binding protein required for 60S ribos... 151 2e-36
CE18041_1 [J] KOG2963 RNA-binding protein required for 60S ribos... 132 9e-31
>YDR312w [J] KOG2963 RNA-binding protein required for 60S ribosomal subunit
biogenesis
Length = 453
Score = 483 bits (1244), Expect = e-136
Identities = 251/382 (65%), Positives = 295/382 (76%), Gaps = 12/382 (3%)
Query: 1 MAKRRTKNRTHVKPSPEELKKIPKSMVIRVGQTSLASHSLNQLVKDFRTIMQPHTAIKLR 60
MAKRR K RTH + +PE+ + IPKSMVIRVGQTSLA+HSLNQLVKDFR IMQPHTA+KL+
Sbjct: 1 MAKRRQKKRTHAQITPEQERDIPKSMVIRVGQTSLANHSLNQLVKDFRQIMQPHTAVKLK 60
Query: 61 ERKSNKLKDFVVMCGPLGVSHLFIFTQSEKTGNVSLKIARTPQGPTITFQVQDYSLGKDI 120
ERKSNKLKDFVVMCGPLGV+HLF+FTQSEKTGNVSLKIARTPQGPT+TFQV DYSLG+DI
Sbjct: 61 ERKSNKLKDFVVMCGPLGVTHLFMFTQSEKTGNVSLKIARTPQGPTVTFQVLDYSLGRDI 120
Query: 121 KKFLKRPKSLSKDDILNPPLLVLNGFQI--KGNEDEEKANVEKVIVSMFQNVFPPLNPAK 178
KKFLKRPKSL+ DD+LNPPLLVLNGF + +ED++ NVEKVIVSMFQN+FPPLNPA+
Sbjct: 121 KKFLKRPKSLNNDDVLNPPLLVLNGFSTSKRSDEDDQDVNVEKVIVSMFQNIFPPLNPAR 180
Query: 179 IQLNSINRVFMINKDQTTGEISLRHYAIDIREVDXXXXXXXXXXXXXXXXXAVPNLHRKD 238
LNSI R+FMINKD+ TGEIS+RHY IDIREV+ VPNLHRK+
Sbjct: 181 TSLNSIKRIFMINKDRETGEISMRHYFIDIREVEISRNLKRLYKAKNNLSKTVPNLHRKE 240
Query: 239 DIASLILDHDVAAYTSESEIEEDAIVKVMDKEHKHIKHIPHRSVNKDTGEKPARLSAXXX 298
DI+SLILDHD+ AYTSESEIE+DAIV+V+D + KH S+++ T P +++
Sbjct: 241 DISSLILDHDLGAYTSESEIEDDAIVRVVDNQDVKAKH-SQTSLSQKT---PVKMT---- 292
Query: 299 XXXXXXXXXXXXXXXXXXXXXXXXQHSTQQPKKKAIKLTEIGPRLTLKLVKIEEGICAGK 358
++S P+KKAIKLTE+GPRLTLKLVKIE+GIC+GK
Sbjct: 293 --DNEEREKGIEEEDVEMEEPKPSENSQPTPRKKAIKLTELGPRLTLKLVKIEDGICSGK 350
Query: 359 VLHHEYVKKSDSEIKALEKRHA 380
VLHHE+V+KS EIKALEKRHA
Sbjct: 351 VLHHEFVQKSSEEIKALEKRHA 372
>YHR066w [J] KOG2963 RNA-binding protein required for 60S ribosomal subunit
biogenesis
Length = 453
Score = 482 bits (1240), Expect = e-136
Identities = 252/382 (65%), Positives = 287/382 (74%), Gaps = 12/382 (3%)
Query: 1 MAKRRTKNRTHVKPSPEELKKIPKSMVIRVGQTSLASHSLNQLVKDFRTIMQPHTAIKLR 60
MAKRR K RTH + +PE+ + IPKSMVIRVGQTSLA+HSLNQLVKDFR IMQPHTAIKL+
Sbjct: 1 MAKRRQKKRTHAQLTPEQEQGIPKSMVIRVGQTSLANHSLNQLVKDFRQIMQPHTAIKLK 60
Query: 61 ERKSNKLKDFVVMCGPLGVSHLFIFTQSEKTGNVSLKIARTPQGPTITFQVQDYSLGKDI 120
ERKSNKLKDFVVMCGPLGV+HLF+FTQSEKTGNVSLKIARTPQGPT+TFQV DYSLG+DI
Sbjct: 61 ERKSNKLKDFVVMCGPLGVTHLFMFTQSEKTGNVSLKIARTPQGPTVTFQVLDYSLGRDI 120
Query: 121 KKFLKRPKSLSKDDILNPPLLVLNGFQI--KGNEDEEKANVEKVIVSMFQNVFPPLNPAK 178
KKFLKRPKSL+ DD+LNPPLLVLNGF + ED++ NVEKVIVSMFQN+FPPLNPA+
Sbjct: 121 KKFLKRPKSLNNDDVLNPPLLVLNGFSTSKRSGEDDQDVNVEKVIVSMFQNIFPPLNPAR 180
Query: 179 IQLNSINRVFMINKDQTTGEISLRHYAIDIREVDXXXXXXXXXXXXXXXXXAVPNLHRKD 238
LNSI RVFMINKD+ TGEIS+RHY IDIREV+ VPNLHRK+
Sbjct: 181 TSLNSIKRVFMINKDRETGEISMRHYFIDIREVEISRNLKRLYKAKNNLSKTVPNLHRKE 240
Query: 239 DIASLILDHDVAAYTSESEIEEDAIVKVMDKEHKHIKHIPHRSVNKDTGEKPARLSAXXX 298
DI+SLILDHD+ AYTSESEIE+DAIV+V+D + KH + EK
Sbjct: 241 DISSLILDHDLGAYTSESEIEDDAIVRVVDNQDVKAKHSQSLKSQRTPVEK--------- 291
Query: 299 XXXXXXXXXXXXXXXXXXXXXXXXQHSTQQPKKKAIKLTEIGPRLTLKLVKIEEGICAGK 358
++ P+KKAIKLTE+GPRLTLKLVKIEEGIC+GK
Sbjct: 292 -KDNKEREKETEEEDVEMEEPKPSENLQPTPRKKAIKLTELGPRLTLKLVKIEEGICSGK 350
Query: 359 VLHHEYVKKSDSEIKALEKRHA 380
VLHHE+V+KS EIKALEKRHA
Sbjct: 351 VLHHEFVQKSSEEIKALEKRHA 372
>SPAC6B12.01 [J] KOG2963 RNA-binding protein required for 60S ribosomal subunit
biogenesis
Length = 361
Score = 204 bits (518), Expect = 3e-52
Identities = 128/355 (36%), Positives = 193/355 (54%), Gaps = 52/355 (14%)
Query: 26 MVIRVGQTSLASHSLNQLVKDFRTIMQPHTAIKLRERKSNKLKDFVVMCGPLGVSHLFIF 85
MVIR G + + SL+ L +D R +M+PHTAI+L+ERK+NK+KD++ M GPLGV+HL +
Sbjct: 1 MVIRSGASEVG-RSLSLLTRDLRHMMEPHTAIRLKERKANKIKDYLTMAGPLGVTHLLVL 59
Query: 86 TQSEKTGNVSLKIARTPQGPTITFQVQDYSLGKDIKKFLKRPKSLSKDDILNPPLLVLNG 145
++++ N +L+I R P+GP++ F++ +Y L KD+++ K PKS + L PPLLV+N
Sbjct: 60 SRTD--NNANLRIIRAPRGPSLHFRIHEYMLNKDVRRLQKNPKS-PTTEFLTPPLLVMNH 116
Query: 146 FQIKGNEDEEKANVEKVIVSMFQNVFPPLNPAKIQLNSINRVFMINKDQTTGEISLRHYA 205
F ++D E ++ + FQN+FPP++ +NS+ RV ++N+ + G I LRH+
Sbjct: 117 FNQNSSKDSPH---EALLTTTFQNMFPPISVQHTNINSVKRVLLLNR-RDDGYIDLRHFI 172
Query: 206 IDIREVDXXXXXXXXXXXXXXXXXAVPNLHRKDDIASLILDHD-VAAYTSESEIEEDAIV 264
I + V +P+LH DI+ +L D ++ S+SEIEEDA V
Sbjct: 173 ISTKPVGISRPIRHLLKGEKKDSD-IPDLHNVRDISDYVLHGDGISGAASDSEIEEDATV 231
Query: 265 KVMDKEHKHIKHIPHRSVNKDTGEKPARLSAXXXXXXXXXXXXXXXXXXXXXXXXXXXQH 324
++ R V T E LSA
Sbjct: 232 EI------------DRPVPTKTEEN--LLSAS---------------------------- 249
Query: 325 STQQPKKKAIKLTEIGPRLTLKLVKIEEGICAGKVLHHEYVKKSDSEIKALEKRH 379
+PK++AIKL EIGPR+TL+L+KI E GKVL+H +V KS EIK + H
Sbjct: 250 QLLKPKQQAIKLIEIGPRMTLELIKITEDAMGGKVLYHSHVHKSKEEIKQQDNFH 304
>Hs9910566 [J] KOG2963 RNA-binding protein required for 60S ribosomal subunit
biogenesis
Length = 473
Score = 168 bits (426), Expect = 1e-41
Identities = 114/353 (32%), Positives = 174/353 (48%), Gaps = 70/353 (19%)
Query: 23 PKSMVIRVGQTSLASHSLNQLVKDFRTIMQPHTAIKLRERKSNKLKDFVVMCGPLGVSHL 82
P S V G T ++ QL D R +M+P TA +L+ RK N LKD V + GPLGV+H
Sbjct: 29 PHSFVFTRGCTG---RNIRQLSLDVRRVMEPLTASRLQVRKKNSLKDCVAVAGPLGVTHF 85
Query: 83 FIFTQSEKTGNVSLKIARTPQGPTITFQVQDYSLGKDIKKFLKRPKSLSKDDILNPPLLV 142
I +++E NV K+ R P GPT+TFQV+ YSL +D+ L+R + + + +PPLLV
Sbjct: 86 LILSKTET--NVYFKLMRLPGGPTLTFQVKKYSLVRDVVSSLRRHR-MHEQQFAHPPLLV 142
Query: 143 LNGFQIKGNEDEEKANVEKVIVSMFQNVFPPLNPAKIQLNSINRVFMINKDQTTGEISLR 202
LN F G K++ +MFQN+FP +N K+ LN+I R +I+ + + E+ R
Sbjct: 143 LNSFGPHGMH-------VKLMATMFQNLFPSINVHKVNLNTIKRCLLIDYNPDSQELDFR 195
Query: 203 HYAIDIREVDXXXXXXXXXXXXXXXXXAVPNLHRKDDIASLILDHDVAAYTSESEIEEDA 262
HY+I + V PN+ R DI+ L+ A SESE E D
Sbjct: 196 HYSIKVVPV------GASRGMKKLLQEKFPNMSRLQDISELLA---TGAGLSESEAEPDG 246
Query: 263 IVKVMDKEHKHIKHIPHRSVNKDTGEKPARLSAXXXXXXXXXXXXXXXXXXXXXXXXXXX 322
+H +I +P +
Sbjct: 247 -------DH-NITELPQAVAGR-------------------------------------- 260
Query: 323 QHSTQQPKKKAIKLTEIGPRLTLKLVKIEEGICAGKVLHHEYVKKSDSEIKAL 375
+ ++ A++LTEIGPR+TL+L+K++EG+ GKV+ H +V K++ E++A+
Sbjct: 261 --GNMRAQQSAVRLTEIGPRMTLQLIKVQEGVGEGKVMFHSFVSKTEEELQAI 311
>7300704 [J] KOG2963 RNA-binding protein required for 60S ribosomal subunit
biogenesis
Length = 460
Score = 157 bits (397), Expect = 3e-38
Identities = 108/373 (28%), Positives = 177/373 (46%), Gaps = 71/373 (19%)
Query: 5 RTKNRTHVKPSPEELKKIPKSMVIRVGQTSLASHSLNQLVKDFRTIMQPHTAIKLRERKS 64
+T+ P E+ + P S VI G LA + L DFR IM+P TA LRE++
Sbjct: 10 KTRTAAFKASEPSEIVEAPHSFVIHRG---LACPYITDLTLDFRRIMEPFTASNLREKRM 66
Query: 65 NKLKDFVVMCGPLGVSHLFIFTQSEKTGNVSLKIARTPQGPTITFQVQDYSLGKDIKKFL 124
N++KDFV + VSH+ IF ++ + +S K+ R P+GP++TF+V ++L +D+ L
Sbjct: 67 NRIKDFVSLSSFFHVSHMGIFNKA--STQLSFKVVRLPRGPSLTFKVHQFTLARDVIS-L 123
Query: 125 KRPKSLSKDDILNPPLLVLNGFQIKGNEDEEKANVEKVIVSMFQNVFPPLNPAKIQLNSI 184
+ + + D + PL+++N F G K++ + FQN+FP +N A + + +I
Sbjct: 124 SKKQMIDNDHFKHAPLVIMNNFSGDGKH-------LKLMATTFQNMFPSINLATVNIGTI 176
Query: 185 NRVFMINKDQTTGEISLRHYAIDIREVDXXXXXXXXXXXXXXXXXAVPNLHRKDDIASLI 244
R + + + T + +RHY++ + V VPNL + +++ +
Sbjct: 177 RRCVLFSYNPDTKLVEMRHYSVQVVPV------GLKRAVQKIVKGTVPNLGKCNEVVDFV 230
Query: 245 LDHDVAAYTSESEIEEDAIVKVMDKEHKHIKHIPHRSVNKDTGEKPARLSAXXXXXXXXX 304
Y SESE E+D E H+ V T + L
Sbjct: 231 TKD---GYASESEAEDD--------EQSHV-------VLAQTLKSKGNL----------- 261
Query: 305 XXXXXXXXXXXXXXXXXXQHSTQQPKKKAIKLTEIGPRLTLKLVKIEEGICAGKVLHHEY 364
+ KK +IKL EIGPRLT++L+KIEEG+ G+VL+H++
Sbjct: 262 -----------------------EDKKSSIKLHEIGPRLTMQLIKIEEGLLTGEVLYHDH 298
Query: 365 VKKSDSEIKALEK 377
V K++ E + L K
Sbjct: 299 VVKTEDEKETLRK 311
>At5g61770 [J] KOG2963 RNA-binding protein required for 60S ribosomal subunit
biogenesis
Length = 305
Score = 151 bits (382), Expect = 2e-36
Identities = 103/339 (30%), Positives = 170/339 (49%), Gaps = 70/339 (20%)
Query: 40 LNQLVKDFRTIMQPHTAIKLRERKSNKLKDFVVMCGPLGVSHLFIFTQSEKTGNVSLKIA 99
+ QL D R +M P+TA+ L+E+K N L+DF+ + GP+GV+H + S+ ++SL++A
Sbjct: 7 VKQLQMDLRKLMLPYTALSLKEKKRNTLRDFLNVSGPMGVTHFLML--SKTASSLSLRVA 64
Query: 100 RTPQGPTITFQVQDYSLGKDIKKFLKRPKSLSKDDILNPPLLVLNGFQIKGNEDEEKANV 159
RTPQGPT+TF++ YSL DI + RP+ +D +PPL+VL+GF G+++
Sbjct: 65 RTPQGPTLTFKIHQYSLASDIAQSQLRPR-CPQDLFKSPPLIVLSGF---GSQELHL--- 117
Query: 160 EKVIVSMFQNVFPPLNPAKIQLNSINRVFMINKDQTTGEISLRHYAIDIREVDXXXXXXX 219
K+ MFQN+FP ++ ++L++ R+ ++N ++ T I RHY+I ++ V
Sbjct: 118 -KLATIMFQNIFPAIDINTVKLSTCQRLVLLNYNKDTKLIDFRHYSIRLQPV-----GVS 171
Query: 220 XXXXXXXXXXAVPNLHRKDDIASLILDHDVAAYTSESEIEEDAIVKVMDKEHKHIKHIPH 279
VP+L D++ + A Y SESE +E+A + + +
Sbjct: 172 RRIRKFVQNHQVPDLRNLQDVSDFVTK---AGYGSESEGDEEAATVTLSSDLGRV----- 223
Query: 280 RSVNKDTGEKPARLSAXXXXXXXXXXXXXXXXXXXXXXXXXXXQHSTQQPKKKAIKLTEI 339
K A SA +KL EI
Sbjct: 224 --------NKGATKSA--------------------------------------VKLQEI 237
Query: 340 GPRLTLKLVKIEEGICAGKVLHHEYVKKSDSEIKALEKR 378
GPR+T++LVK+EEG+C G ++ EY + D + + L+K+
Sbjct: 238 GPRMTMQLVKVEEGLCTGGIIFSEY-EDVDGKKEKLKKK 275
>CE18041_1 [J] KOG2963 RNA-binding protein required for 60S ribosomal subunit
biogenesis
Length = 400
Score = 132 bits (333), Expect = 9e-31
Identities = 102/358 (28%), Positives = 170/358 (46%), Gaps = 35/358 (9%)
Query: 18 ELKKIPKSMVIRVGQTSLASHSLNQLVKDFRTIMQPHTAIKLRERKSNKLKDFVVMCGPL 77
EL + P +VI G + L D R +++P+TA L+ K N +KDF+V L
Sbjct: 51 ELARQPHCLVIHRGDVG---KYVKGLESDLRNLVEPNTAKNLKILKRNNIKDFIVNGAVL 107
Query: 78 GVSHLFIFTQSEKTGNVSLKIARTPQGPTITFQVQDYSLGKDIKKFLKRPKSLSKDDILN 137
GV+++ + T S+ ++ L++ R QGPT++F+V+ YSL + + KRP + K +
Sbjct: 108 GVTNMMVLTSSD--ASLQLRMMRFSQGPTLSFKVKQYSLARHVVNCQKRPVATDKL-FKS 164
Query: 138 PPLLVLNGFQIKGNEDEEKANVEKVIVSMFQNVFPPLNPAKIQLNSINRVFMINKDQTTG 197
PL+V+NGF G+ ++ ++ + + QN+FP +N IQL ++ R +++ D+ T
Sbjct: 165 SPLVVMNGF---GDGTQKHLSLVQTFI---QNMFPSINVDTIQLGNLKRCLIVSYDEETD 218
Query: 198 EISLRHYAIDIREVDXXXXXXXXXXXXXXXXXAVPNLHRKDDIASLILD---HDVAAYTS 254
EI +RH+AI + +PNL DI+ L+ + +
Sbjct: 219 EIQMRHFAIRVVASGLNKSVKKLMQAEKTMGKNIPNLSTYKDISDYFLNPGQFRIRTKLN 278
Query: 255 ESEIEEDAIVKVMDKEHKHIKHIPHRSVNKDTGEKPARLSAXXXXXXXXXXXXXXXXXXX 314
S I I+K K+ ++ N + P +LS
Sbjct: 279 FSNINHKIIIK------KYSIYL-----NFPSFSSPGQLS---------DSEFEGDQQEV 318
Query: 315 XXXXXXXXQHSTQQPKKKAIKLTEIGPRLTLKLVKIEEGICAGKVLHHEYVKKSDSEI 372
+K ++L EIGPRLTL+LVKIEEGI G+VL+H++ K+ E+
Sbjct: 319 ELPQDISEGRGCGVGQKSNVRLHEIGPRLTLELVKIEEGIDEGEVLYHKHNAKTPDEL 376
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.133 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,400,659
Number of Sequences: 60738
Number of extensions: 787614
Number of successful extensions: 1701
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1669
Number of HSP's gapped (non-prelim): 16
length of query: 474
length of database: 30,389,216
effective HSP length: 110
effective length of query: 364
effective length of database: 23,708,036
effective search space: 8629725104
effective search space used: 8629725104
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)