ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII3663  suspect: LH   K     KOG1510  Transcription                                                  RNA polymerase II holoenzyme and mediator subcomplex, subunit SURB7/SRB7

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII3663 1291927  1292445 173  
         (173 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDR308c [K] KOG1510 RNA polymerase II holoenzyme and mediator su...   175   2e-44
SPBC1604.10 [K] KOG1510 RNA polymerase II holoenzyme and mediato...    64   8e-11

>YDR308c [K] KOG1510 RNA polymerase II holoenzyme and mediator subcomplex
           subunit SURB7/SRB7
          Length = 140

 Score =  175 bits (444), Expect = 2e-44
 Identities = 89/137 (64%), Positives = 106/137 (76%)

Query: 31  MTDRLTQLQICLDQMMEQFCGAVNYVDKNHGFEPSNDSEEQMSDPQAHIVEEKEFEKNID 90
           MTDRLTQLQICLDQM EQFC  +NY+DKNHGFE    +E QMSD  A +V  +EF   ID
Sbjct: 1   MTDRLTQLQICLDQMTEQFCATLNYIDKNHGFERLTVNEPQMSDKHATVVPPEEFSNTID 60

Query: 91  ELTTDIILKTRQIMALIDSLPGVDVSAQEQLHRIDSLQKQLIKMEKEKIDAIKRKDALQE 150
           EL+TDIILKTRQI  LIDSLPGVDVSA+EQL +ID LQK+L+++E EKI+AIK+K+ L  
Sbjct: 61  ELSTDIILKTRQINKLIDSLPGVDVSAEEQLRKIDMLQKKLVEVEDEKIEAIKKKEKLLR 120

Query: 151 KVRTLTQDFTIGINESK 167
            V +L +DF  GI  SK
Sbjct: 121 HVDSLIEDFVDGIANSK 137


>SPBC1604.10 [K] KOG1510 RNA polymerase II holoenzyme and mediator subcomplex
           subunit SURB7/SRB7
          Length = 138

 Score = 64.3 bits (155), Expect = 8e-11
 Identities = 33/108 (30%), Positives = 61/108 (55%), Gaps = 1/108 (0%)

Query: 31  MTDRLTQLQICLDQMMEQFCGAVNYVDKNHGFEPSNDSEEQMSDPQAHIVEEKEFEKNID 90
           M  R TQLQ  +D++  QF  +++Y+  +H F P    +E++SD + + +  +E +    
Sbjct: 1   MACRCTQLQDTIDEVATQFYSSIHYLSSHHDFVPL-PGQEKVSDSKVNPISAEELQFAQR 59

Query: 91  ELTTDIILKTRQIMALIDSLPGVDVSAQEQLHRIDSLQKQLIKMEKEK 138
           +L  D++ K  QI  LI+ LPG+  + + QL +I  LQ  + + + E+
Sbjct: 60  DLAKDLVTKFMQIDTLINQLPGISTAPKHQLEKIKKLQNSIEEKQLER 107


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.315    0.131    0.349 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,578,435
Number of Sequences: 60738
Number of extensions: 389237
Number of successful extensions: 1649
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1647
Number of HSP's gapped (non-prelim): 2
length of query: 173
length of database: 30,389,216
effective HSP length: 99
effective length of query: 74
effective length of database: 24,376,154
effective search space: 1803835396
effective search space used: 1803835396
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)