ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3682 good O KOG3800 Posttranslational modification, protein turnover, chaperones Predicted E3 ubiquitin ligase containing RING finger, subunit of transcription/repair factor TFIIH and CDK-activating kinase assembly factor
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3682 1297342 1298295 318
(318 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR460w [O] KOG3800 Predicted E3 ubiquitin ligase containing RIN... 457 e-129
SPBC776.18c [O] KOG3800 Predicted E3 ubiquitin ligase containing... 216 3e-56
7303708 [O] KOG3800 Predicted E3 ubiquitin ligase containing RIN... 137 2e-32
Hs4505225 [O] KOG3800 Predicted E3 ubiquitin ligase containing R... 127 2e-29
ECU11g0220 [O] KOG3800 Predicted E3 ubiquitin ligase containing ... 101 1e-21
CE11002_2 [O] KOG3800 Predicted E3 ubiquitin ligase containing R... 77 3e-14
>YDR460w [O] KOG3800 Predicted E3 ubiquitin ligase containing RING finger
subunit of transcription/repair factor TFIIH and
CDK-activating kinase assembly factor
Length = 321
Score = 457 bits (1177), Expect = e-129
Identities = 225/321 (70%), Positives = 264/321 (82%), Gaps = 8/321 (2%)
Query: 1 MDE-DEKKDMCPICKTDRYLSPDMKFLINPECYHKICESCVDRIFSLGPAQCPYEGCDKI 59
MDE +E KDMCPICKTDRYLSPD+KFL+NPECYH+ICESCVDRIFSLGPAQCPY+GCDKI
Sbjct: 3 MDEYEENKDMCPICKTDRYLSPDVKFLVNPECYHRICESCVDRIFSLGPAQCPYKGCDKI 62
Query: 60 LRRNKFKTQIFDDVGVEKEVDIRKRVFNVFNKTLEDFDNNQDDYDKYLEEVEDIIYNLDH 119
LR+NKFKTQIFDDV VEKEVDIRKRVFNVFNKT++DF+ + +Y+KYLEEVEDIIY LDH
Sbjct: 63 LRKNKFKTQIFDDVEVEKEVDIRKRVFNVFNKTIDDFNGDLVEYNKYLEEVEDIIYKLDH 122
Query: 120 GIDMEKTEERLRTYEELNKQLIMANLERSKQDVANFEQRQXXXXXXXXXXXXXXXQIEEE 179
GID+ KTEE+LRTYEELNKQLIM NLERS+ ++ +FEQRQ QIEEE
Sbjct: 123 GIDVAKTEEKLRTYEELNKQLIMNNLERSRTEIESFEQRQKFEKEMKLKKRLLERQIEEE 182
Query: 180 ERANREWAKREIVNQL-TVNEDAEEVIENVKKTVKLKKSSARRKLEELNKVLRNNPY--- 235
ER N+EW K+EIVN+L T +D E IE VK TVKLKKSSARRKLEELN+VL+NNPY
Sbjct: 183 ERMNKEWTKKEIVNRLSTTTQDINETIEGVKNTVKLKKSSARRKLEELNRVLKNNPYFNS 242
Query: 236 -MTMTNGRMKKKDTVPFTPFNGDRDLHKRYELVENEYNDSFIRDLQKKKEYIASGFRADF 294
+ + N R+ KD VPFTPFNGDR+ H R+ L + YND FI+DL+ +KE+IASGF ++
Sbjct: 243 NVNVQNSRL--KDAVPFTPFNGDREAHPRFTLKGSVYNDPFIKDLEHRKEFIASGFNTNY 300
Query: 295 IYDRMLTEAFMGLGCIISEEL 315
Y+R+LTEAFMGLGC+ISEEL
Sbjct: 301 AYERVLTEAFMGLGCVISEEL 321
>SPBC776.18c [O] KOG3800 Predicted E3 ubiquitin ligase containing RING finger
subunit of transcription/repair factor TFIIH and
CDK-activating kinase assembly factor
Length = 318
Score = 216 bits (550), Expect = 3e-56
Identities = 113/309 (36%), Positives = 188/309 (60%), Gaps = 10/309 (3%)
Query: 2 DEDEKK--DMCPICKTDRYLSPDMKFLINPECYHKICESCVDRIFSLGPAQCPYEGCDKI 59
DE +K + CP+C+ DRYL+P+MK LINPECYHK+CESCVDRIF+ GPAQCP GC+KI
Sbjct: 3 DEGARKVDEKCPLCQADRYLNPNMKLLINPECYHKMCESCVDRIFTTGPAQCPTPGCNKI 62
Query: 60 LRRNKFKTQIFDDVGVEKEVDIRKRVFNVFNKTLEDFDNNQDDYDKYLEEVEDIIYNLDH 119
LR+ KF+ Q F+D +E+EVD+RKR+ +FNK ++FD+ Q Y+ YLEEVE + +NL +
Sbjct: 63 LRKAKFREQTFEDAQIEREVDVRKRISRIFNKGQQEFDSLQ-AYNDYLEEVEILTFNLIY 121
Query: 120 GIDMEKTEERLRTYEELNKQLIMANLERSKQDVANFEQRQXXXXXXXXXXXXXXXQIEEE 179
ID+E+TEE+++ YE+ N+ I AN R+ + Q + + ++
Sbjct: 122 KIDVEETEEKVKQYEKQNRDSIAANSARAAAEARILAQNEILLKRQKQEAREAAIREHQK 181
Query: 180 ERANREWAKREIVNQL-TVNEDAEEVIENVKKTVKLKKSSARRKLEELNKVLRNNPYMTM 238
E+ RE +++I+ L T +D ++I+ + ++K ++ + + +++ + + +
Sbjct: 182 EKERREQVEQQIIFDLATSGKDPNKIIQ-LSDSLKKQQENIASSVSNISR----SSSILL 236
Query: 239 TNGRMKKKDTVPFTPFNGDRDLHKRYELVENEYNDSFIRDLQKKKEYIASGFRADFIYDR 298
++ + +DT PF+P G++D K + +N Y D ++ + + GFR + + R
Sbjct: 237 SDVQQVAEDTTPFSPLAGEKDGSKYFSYSKNTYQDLYLEKVSHEPGR-KCGFRIEDCHLR 295
Query: 299 MLTEAFMGL 307
L EAF G+
Sbjct: 296 CLYEAFSGI 304
>7303708 [O] KOG3800 Predicted E3 ubiquitin ligase containing RING finger
subunit of transcription/repair factor TFIIH and
CDK-activating kinase assembly factor
Length = 320
Score = 137 bits (345), Expect = 2e-32
Identities = 92/310 (29%), Positives = 153/310 (48%), Gaps = 19/310 (6%)
Query: 10 CPICKTDRYLSPDMKFLINPECYHKICESCVDRIFSLGPAQCPYEGCDKILRRNKFKTQI 69
CP CKT +Y +P +K ++N C H +CESCVD +F G CP C LRRN F+ Q+
Sbjct: 6 CPRCKTTKYRNPSLKLMVNV-CGHTLCESCVDLLFLKGSGACPE--CMVPLRRNNFRVQL 62
Query: 70 FDDVGVEKEVDIRKRVFNVFNKTLEDFDNNQDDYDKYLEEVEDIIYNLDHGIDMEKTEER 129
F+D VEKEVDIR+R+ +NK EDF + + Y+ YLEE+EDI+YNL + ID+ +T +R
Sbjct: 63 FEDPMVEKEVDIRRRILRDYNKREEDFASLAE-YNDYLEEIEDIVYNLCNNIDIIETNKR 121
Query: 130 LRTYEELNKQLIMANLERSKQDVANFEQRQXXXXXXXXXXXXXXXQIEEEERANREWAKR 189
+ Y+ N+++I N R +D E+ ++E E + + K+
Sbjct: 122 IEAYKRDNREVIQRNKTRVGRDEYALEEMLELEKVQEEARRKELEELENEHKKKKARDKQ 181
Query: 190 EIVNQLTVN-EDAEEVI-ENVKKTVKLKKSSAR----RKLEELNKVLRNNPYMTMTNGRM 243
++ +L + +DA +++ E +K K ++ + + E + ++ + +
Sbjct: 182 ALIEELMYSGKDAAQIVTEFAEKAEKQREEEKQLPPPKPANEFSTGIKFGQTADPSLLPV 241
Query: 244 KKKDT------VPFTPFNGDRDLHKRYELVENEYNDSFIRDLQKKKEYIASGFRADFIYD 297
K + P PF+ + E+V Y + ++ A GF + +
Sbjct: 242 PKSEEGPLFVYEPLVPFSEGPAMPPTNEIVSRGYIAHIRAETPQEN---AGGFTSALACE 298
Query: 298 RMLTEAFMGL 307
R L EA GL
Sbjct: 299 RALQEALQGL 308
>Hs4505225 [O] KOG3800 Predicted E3 ubiquitin ligase containing RING finger
subunit of transcription/repair factor TFIIH and
CDK-activating kinase assembly factor
Length = 309
Score = 127 bits (319), Expect = 2e-29
Identities = 83/318 (26%), Positives = 158/318 (49%), Gaps = 39/318 (12%)
Query: 10 CPICKTDRYLSPDMKFLINPECYHKICESCVDRIFSLGPAQCPYEGCDKILRRNKFKTQI 69
CP CKT +Y +P +K ++N C H +CESCVD +F G CP C LR++ F+ Q+
Sbjct: 6 CPRCKTTKYRNPSLKLMVNV-CGHTLCESCVDLLFVRGAGNCPE--CGTPLRKSNFRVQL 62
Query: 70 FDDVGVEKEVDIRKRVFNVFNKTLEDFDNNQDDYDKYLEEVEDIIYNLDHGIDMEKTEER 129
F+D V+KEV+IRK+V ++NK EDF + + +Y+ +LEEVE+I++NL + +D++ T+++
Sbjct: 63 FEDPTVDKEVEIRKKVLKIYNKREEDFPSLR-EYNDFLEEVEEIVFNLTNNVDLDNTKKK 121
Query: 130 LRTYEELNKQLIMANLERSKQDVANFEQRQXXXXXXXXXXXXXXXQIEEEERANREW--A 187
+ Y++ NK +I N + ++ E+ ++E +E R
Sbjct: 122 MEIYQKENKDVIQKNKLKLTREQEELEE---------------ALEVERQENEQRRLFIQ 166
Query: 188 KREIVNQLTVNEDAEEVIENVKKT---VKLKKSSARRKLEELNKVL-RNNPYMTMT-NGR 242
K E + Q+ ++ + ++ ++ + V L + + + +L L + P +T +
Sbjct: 167 KEEQLQQILKRKNKQAFLDELESSDLPVALLLAQHKDRSTQLEMQLEKPKPVKPVTFSTG 226
Query: 243 MKKKDTVPFTPFNGDRDLHKRYELVENEYNDSFIRDLQ-------------KKKEYIASG 289
+K + P + + Y+ ++ E + +L+ + +A G
Sbjct: 227 IKMGQHISLAPIHKLEEALYEYQPLQIETYGPHVPELEMLGRLGYLNHVRAASPQDLAGG 286
Query: 290 FRADFIYDRMLTEAFMGL 307
+ + R L +AF GL
Sbjct: 287 YTSSLACHRALQDAFSGL 304
>ECU11g0220 [O] KOG3800 Predicted E3 ubiquitin ligase containing RING finger
subunit of transcription/repair factor TFIIH and
CDK-activating kinase assembly factor
Length = 227
Score = 101 bits (252), Expect = 1e-21
Identities = 47/133 (35%), Positives = 82/133 (61%), Gaps = 3/133 (2%)
Query: 10 CPICKTDRYLSPDMKFLINPECYHKICESCVDRIFSLGPAQCPYEGCDKILRRNKFKTQI 69
CP+CK+D YL+PD+ I+P C+H++CESC+ RIFS G A CP C ILR+ +
Sbjct: 6 CPVCKSDIYLNPDIVIYISP-CFHRMCESCMFRIFSRGQAPCPE--CGTILRKINYIKPT 62
Query: 70 FDDVGVEKEVDIRKRVFNVFNKTLEDFDNNQDDYDKYLEEVEDIIYNLDHGIDMEKTEER 129
F+D+ VEKE +R+ + F + E+F + Y+ YLE+ E++++ + + +E+
Sbjct: 63 FEDITVEKECKVRRILLRAFGREEEEFGGDSAKYNDYLEKFEELVFEVLELKNESLVKEK 122
Query: 130 LRTYEELNKQLIM 142
++ +E+ + I+
Sbjct: 123 VKKIQEMGQDSIL 135
>CE11002_2 [O] KOG3800 Predicted E3 ubiquitin ligase containing RING finger
subunit of transcription/repair factor TFIIH and
CDK-activating kinase assembly factor
Length = 403
Score = 77.0 bits (188), Expect = 3e-14
Identities = 34/98 (34%), Positives = 63/98 (63%), Gaps = 5/98 (5%)
Query: 10 CPICKTDRYLSPDMKFLINPECYHKICESCVDRIFSLGPAQCPYEGCDKILRRNKFKTQI 69
C CK++ Y + + +IN EC H +C++CVD IF+L C C+++LR+N F+ QI
Sbjct: 3 CKKCKSNEYTNKQLVMMIN-ECGHPLCKNCVDNIFALNSGNCHV--CNRVLRKNNFREQI 59
Query: 70 FDDVGVEKEVDIRKRVFNVFNKTLEDFDNNQD--DYDK 105
++D ++KE +R+++ ++N +DF+N ++ DY +
Sbjct: 60 YEDPLIDKETFLRRKLRKIYNLKQDDFENLREFGDYQE 97
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,264,623
Number of Sequences: 60738
Number of extensions: 869442
Number of successful extensions: 4281
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 4262
Number of HSP's gapped (non-prelim): 7
length of query: 318
length of database: 30,389,216
effective HSP length: 106
effective length of query: 212
effective length of database: 23,950,988
effective search space: 5077609456
effective search space used: 5077609456
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)