ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII3735  suspect: LH   U     KOG1792  Intracellular trafficking, secretion, and vesicular transport  Reticulon

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII3735 1315161 1314307 -285 
         (285 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDR233c [U] KOG1792 Reticulon                                         246   2e-65
YDL204w [U] KOG1792 Reticulon                                         125   7e-29

>YDR233c [U] KOG1792 Reticulon
          Length = 295

 Score =  246 bits (629), Expect = 2e-65
 Identities = 122/242 (50%), Positives = 168/242 (69%), Gaps = 4/242 (1%)

Query: 12  CDILYWKNPVETGKXXXXXXXXXXXIKKVNLISLFLKLLYTVLFATASVEFLSKLILNKG 71
           CD+L W+NPV+TGK           +KKVNLI+ FLK+ YT+LF T S+EF+SKL L +G
Sbjct: 21  CDLLLWRNPVQTGKYFGGSLLALLILKKVNLITFFLKVAYTILFTTGSIEFVSKLFLGQG 80

Query: 72  LVTTYGIKECPDIAGTIKPYIDEALKQLPVYQAKFRKLVFASSPECSFKAAGVVYIAHKL 131
           L+T YG KECP+IAG IKP+IDEALKQLPV+QA  RK VFA  P+ +FK A  +++ HK 
Sbjct: 81  LITKYGPKECPNIAGFIKPHIDEALKQLPVFQAHIRKTVFAQVPKHTFKTAVALFLLHKF 140

Query: 132 ISFLSLWTIALVSTLATFSVPILYKTYQKEIDAAVEQGVSVAKTKSSEYQKLVQEKATPY 191
            S+ S+WTI  V+ + TF++P++Y +Y+ EIDA V QGV ++K K+ E+ ++  EK  PY
Sbjct: 141 FSWFSIWTIVFVADIFTFTLPVIYHSYKHEIDATVAQGVEISKQKTQEFSQMACEKTKPY 200

Query: 192 VKQLDEKLGPVSGFVKSKIP--QSRVTPE-STTAKLAADVPLESNEQSTATTSGASFPSV 248
           + +++ KLGP+S  VKSK     S   P+ ++T+KLAADVPLE  E    T+S    P V
Sbjct: 201 LDKVESKLGPISNLVKSKTAPVSSTAGPQTASTSKLAADVPLEP-ESKAYTSSAQVMPEV 259

Query: 249 PK 250
           P+
Sbjct: 260 PQ 261


>YDL204w [U] KOG1792 Reticulon
          Length = 393

 Score =  125 bits (314), Expect = 7e-29
 Identities = 58/191 (30%), Positives = 110/191 (57%)

Query: 5   KVSEKSQCDILYWKNPVETGKXXXXXXXXXXXIKKVNLISLFLKLLYTVLFATASVEFLS 64
           +   +++  ++YW NP ++G            ++ VN+IS+ LK+ Y VLF + +VE  +
Sbjct: 24  EAGHQNKTGLIYWTNPSKSGASFAATLVSLLILRNVNVISVLLKIGYMVLFTSFAVELST 83

Query: 65  KLILNKGLVTTYGIKECPDIAGTIKPYIDEALKQLPVYQAKFRKLVFASSPECSFKAAGV 124
           K++ +KG+V+ +G++E PD+ G +KP+ID  L +LP  + + RKLVFA     +F     
Sbjct: 84  KVLFDKGVVSRFGMQESPDLVGVLKPHIDRELDRLPALEDRIRKLVFAHRTRNNFTIGVS 143

Query: 125 VYIAHKLISFLSLWTIALVSTLATFSVPILYKTYQKEIDAAVEQGVSVAKTKSSEYQKLV 184
           +Y  H L +  S+ T+ +++T+  ++VP++Y   Q  ID A+++   +   +  +    V
Sbjct: 144 LYFLHGLFAIFSMNTVLIMTTIFLYTVPLIYDRKQARIDRAIDRMKDLVIHRFHKNYNKV 203

Query: 185 QEKATPYVKQL 195
            EK  PY+ ++
Sbjct: 204 VEKTEPYIDKI 214


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.314    0.130    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,349,223
Number of Sequences: 60738
Number of extensions: 470394
Number of successful extensions: 1262
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1259
Number of HSP's gapped (non-prelim): 2
length of query: 285
length of database: 30,389,216
effective HSP length: 105
effective length of query: 180
effective length of database: 24,011,726
effective search space: 4322110680
effective search space used: 4322110680
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)