ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3745 good H KOG2892 Coenzyme transport and metabolism Porphobilinogen deaminase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3745 1319226 1320206 327
(327 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL205c [H] KOG2892 Porphobilinogen deaminase 434 e-122
Hs20149500 [H] KOG2892 Porphobilinogen deaminase 213 3e-55
7292071 [H] KOG2892 Porphobilinogen deaminase 187 2e-47
At5g08280 [H] KOG2892 Porphobilinogen deaminase 149 7e-36
SPAC24B11.13 [H] KOG2892 Porphobilinogen deaminase 100 4e-21
>YDL205c [H] KOG2892 Porphobilinogen deaminase
Length = 327
Score = 434 bits (1117), Expect = e-122
Identities = 218/323 (67%), Positives = 261/323 (80%), Gaps = 1/323 (0%)
Query: 5 ETVRIGGRRSKLAVVQSEQVKVMIESKFSHIECPLLSVHTLGDQVQSKPLYSFGGKAVWT 64
ET+ IGGR+SKLAV+QS V +IE K+ +C + ++ TLGDQ+Q KPLYSFGGKA+WT
Sbjct: 4 ETLHIGGRKSKLAVIQSNHVLKLIEEKYPDYDCKVFTLQTLGDQIQFKPLYSFGGKALWT 63
Query: 65 KELEDLLYKDDESR-IDLIVHSLKDMPTLLPDGFELGGITKRVDPTDALVMPIGSPYSSL 123
KELED LY DD S+ +DLIVHSLKDMPTLLP+GFELGGITKRVDPTD LVMP S Y SL
Sbjct: 64 KELEDHLYHDDPSKKLDLIVHSLKDMPTLLPEGFELGGITKRVDPTDCLVMPFYSAYKSL 123
Query: 124 SELPDXXXXXXXXXXXXAQLKRKFPNLKFESIRGNIQTRLAKLDDPETPYKCIVLASAGL 183
+LPD AQLKRK+P+LKFES+RGNIQTRL KLDDP++PY+CI+LASAGL
Sbjct: 124 DDLPDGGIVGTSSVRRSAQLKRKYPHLKFESVRGNIQTRLQKLDDPKSPYQCIILASAGL 183
Query: 184 MRSGLDSRITQRFNADTMCYAVGQGALGIEIRKDDEKMKKILKEICDPSTTICCLAERSL 243
MR GL++RITQRF++DTM +AVGQGALGIEIRK D KM KIL EICD + TICCL+ER+L
Sbjct: 184 MRMGLENRITQRFHSDTMYHAVGQGALGIEIRKGDTKMMKILDEICDLNATICCLSERAL 243
Query: 244 LRTLEGGCSVPIGVVSNYDESTKVLTLKGIVINVEGTEWVEIEHKVTISNEREDSINCGK 303
+RTLEGGCSVPIGV S Y+E TK L LK IV++VEGTE VE E ++ I N +EDS+ CGK
Sbjct: 244 MRTLEGGCSVPIGVESKYNEETKKLLLKAIVVDVEGTEAVEDEIEMLIENVKEDSMACGK 303
Query: 304 ELAAKLTQNGAKEILDSINLDKI 326
LA ++ +GAK+ILD INLD+I
Sbjct: 304 ILAERMIADGAKKILDEINLDRI 326
>Hs20149500 [H] KOG2892 Porphobilinogen deaminase
Length = 361
Score = 213 bits (542), Expect = 3e-55
Identities = 124/338 (36%), Positives = 196/338 (57%), Gaps = 31/338 (9%)
Query: 7 VRIGGRRSKLAVVQSEQVKVMIESKFSHIECPLLSVHTLGDQVQSKPLYSFGGKAVWTKE 66
+R+G R+S+LA +Q++ V +++ + ++ ++++ T GD++ L G K+++TKE
Sbjct: 21 IRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKE 80
Query: 67 LEDLLYKDDESRIDLIVHSLKDMPTLLPDGFELGGITKRVDPTDALVMPIGSPYSSLSEL 126
LE L K++ +DL+VHSLKD+PT+LP GF +G I KR +P DA+V +L L
Sbjct: 81 LEHALEKNE---VDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETL 137
Query: 127 PDXXXXXXXXXXXXAQLKRKFPNLKFESIRGNIQTRLAKLDDPETPYKCIVLASAGLMRS 186
P+ AQL+RKFP+L+F SIRGN+ TRL KLD+ + + I+LA+AGL R
Sbjct: 138 PEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDE-QQEFSAIILATAGLQRM 196
Query: 187 GLDSRITQRFNADTMCYAVGQGALGIEIRKDDEKMKKILKEICDPSTTICCLAERSLLRT 246
G +R+ Q + + YAVGQGALG+E+R D+ + ++ + DP T + C+AER+ LR
Sbjct: 197 GWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRH 256
Query: 247 LEGGCSVPIGVVSNYDESTKVLTLKGIVINVEGTEWVE--IEHKVTISNERED------- 297
LEGGCSVP+ V + + LT G V +++G++ ++ ++ + + + ED
Sbjct: 257 LEGGCSVPVAVHTAMKDGQLYLT--GGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQ 314
Query: 298 ----------------SINCGKELAAKLTQNGAKEILD 319
+ N G LA L GAK ILD
Sbjct: 315 LVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILD 352
>7292071 [H] KOG2892 Porphobilinogen deaminase
Length = 652
Score = 187 bits (475), Expect = 2e-47
Identities = 108/313 (34%), Positives = 180/313 (57%), Gaps = 5/313 (1%)
Query: 4 KETVRIGGRRSKLAVVQSEQVKVMIESKFSHIECPLLSVHTLGDQVQSKPLYSFGGKAVW 63
++ +R+G R+S+LA++Q++ V ++ + + + ++ T GD+V + L G K+++
Sbjct: 5 EKVIRVGSRKSELALIQTKHVIGRLQKLYPKQKFEIHTMSTFGDRVLNVSLPKIGEKSLF 64
Query: 64 TKELEDLLYKDDESRIDLIVHSLKDMPTLLPDGFELGGITKRVDPTDALVMPIGSPYSSL 123
T++LED L +D +VHSLKD+PT LP G +G + +R D DALV+ ++
Sbjct: 65 TRDLEDALRNGG---VDFVVHSLKDLPTALPTGMAIGAVLEREDARDALVLRENFKGHTI 121
Query: 124 SELPDXXXXXXXXXXXXAQLKRKFPNLKFESIRGNIQTRLAKLDDPETPYKCIVLASAGL 183
+ LP AQ++R +P+L IRGN+ TRLAKLD ++ + I+LA AGL
Sbjct: 122 ASLPKGSVIGTSSLRRTAQIRRMYPHLTVCDIRGNLNTRLAKLDAADSKFSGIILAQAGL 181
Query: 184 MRSGLDSRITQRFNADTMCYAVGQGALGIEIRKDDEKMKKILKEICDPSTTICCLAERSL 243
+R G SRI+Q + YAVGQGAL +E R +D+++ +L+++ +TT LAERS
Sbjct: 182 VRMGWMSRISQVLEPTDLLYAVGQGALAVECRANDDQVLAMLQKLMCLNTTCRILAERSF 241
Query: 244 LRTLEGGCSVPIGVVSNYDESTKVLTLKGIVINVEGTEWVEIEHKVTISNEREDSINCGK 303
L+TL GGCS P+ V SN + + +++ G W ++ + I N ++N +
Sbjct: 242 LKTLGGGCSAPVAVWSNLKGEPLNGNSQEVGLSLTGAVW-SLDGAIEIRNHLACALN-EQ 299
Query: 304 ELAAKLTQNGAKE 316
+L + GA+E
Sbjct: 300 KLEGDQRKRGAQE 312
>At5g08280 [H] KOG2892 Porphobilinogen deaminase
Length = 382
Score = 149 bits (375), Expect = 7e-36
Identities = 103/312 (33%), Positives = 157/312 (50%), Gaps = 24/312 (7%)
Query: 7 VRIGGRRSKLAVVQSEQVKVMIESKFS--------HIECPLLSVHTLGDQVQSKPLYSFG 58
+RIG R S LA+ Q+ + + ++ K HIE + T GD++ S+PL G
Sbjct: 75 IRIGTRGSPLALAQAYETREKLKKKHPELVEDGAIHIEI----IKTTGDKILSQPLADIG 130
Query: 59 GKAVWTKELEDLLYKDDESRIDLIVHSLKDMPTLLPDGFELGGITKRVDPTDALVMPIGS 118
GK ++TKE+++ L ID+ VHS+KD+PT LP+ L R D DA + +
Sbjct: 131 GKGLFTKEIDEALIN---GHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAA 187
Query: 119 PYSSLSELPDXXXXXXXXXXXXAQLKRKFPNLKFE-SIRGNIQTRLAKLDDPETPYKCIV 177
+L+ELP +Q+ K+P L E + RGN+QTRL+KL + + +
Sbjct: 188 ---TLAELPAGSVVGTASLRRKSQILHKYPALHVEENFRGNVQTRLSKLQGGKV--QATL 242
Query: 178 LASAGLMRSGLDSRITQRFNADTMCYAVGQGALGIEIRKDDEKMKKILKEICDPSTTICC 237
LA AGL R + + + D M AV QGA+GI R DD+KM L + T +
Sbjct: 243 LALAGLKRLSMTENVASILSLDEMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAI 302
Query: 238 LAERSLLRTLEGGCSVPIGVVSNYDESTKVLTLKGIVINVEGTEWVEIEHKVTISNERED 297
ER+ L TL+G C PI ++ DE + +G+V + +GT+ +E K ED
Sbjct: 303 SCERAFLETLDGSCRTPIAGYASKDEEGNCI-FRGLVASPDGTKVLETSRKGPY--VYED 359
Query: 298 SINCGKELAAKL 309
+ GK+ +L
Sbjct: 360 MVKMGKDAGQEL 371
>SPAC24B11.13 [H] KOG2892 Porphobilinogen deaminase
Length = 125
Score = 100 bits (248), Expect = 4e-21
Identities = 53/128 (41%), Positives = 83/128 (64%), Gaps = 6/128 (4%)
Query: 1 MESKETVRIGGRRSKLAVVQSEQVKVMIESKFSHIECPLLSVHTLGDQVQSKPLYSFG-- 58
M S + IG R+SKLAV+QSE ++ +E + H+E P++S T+GD++ SK L+ F
Sbjct: 1 MPSCTSFPIGTRKSKLAVIQSEIIREELEKHYPHLEFPIISRDTIGDEILSKALFEFKRQ 60
Query: 59 -GKAVWTKELEDLLYKDDESRIDLIVHSLKDMPTLLPDGFELGGITKRVDPTDALVMPIG 117
K++WT+ELE LL ++ ++VHSLKD+P+ +PDG + I KR P DA+V G
Sbjct: 61 LAKSLWTRELEALLV---TNQCRILVHSLKDLPSEMPDGMVIACIPKRSCPLDAIVFKAG 117
Query: 118 SPYSSLSE 125
S Y ++++
Sbjct: 118 SHYKTVAD 125
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.135 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,526,256
Number of Sequences: 60738
Number of extensions: 764548
Number of successful extensions: 2028
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2013
Number of HSP's gapped (non-prelim): 5
length of query: 327
length of database: 30,389,216
effective HSP length: 107
effective length of query: 220
effective length of database: 23,890,250
effective search space: 5255855000
effective search space used: 5255855000
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)