ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3749 good A KOG1258 RNA processing and modification mRNA processing protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3749 1320694 1322340 549
(549 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR235w [A] KOG1258 mRNA processing protein 473 e-133
SPBC4B4.09 [A] KOG1258 mRNA processing protein 80 8e-15
YML046w [A] KOG1258 mRNA processing protein 70 6e-12
At1g04080 [A] KOG1258 mRNA processing protein 52 2e-06
>YDR235w [A] KOG1258 mRNA processing protein
Length = 544
Score = 473 bits (1216), Expect = e-133
Identities = 229/537 (42%), Positives = 337/537 (62%), Gaps = 1/537 (0%)
Query: 7 LLGDQKLSEFSLLICKYPKSXXXXXXXXXXXXXKASPISKSINKDLLQLVRSTYESLLYQ 66
L+ D+ S +L + +YPKS ++PI KS LL+L+R TY S+L +
Sbjct: 7 LIHDENFSTLTLNVSRYPKSLAYWEKLLNYIVKASAPICKSTEPQLLKLIRCTYSSMLNE 66
Query: 67 FPFLENYHVEYGLLEYKLGNFGKFHAIFENALQYFNNRSLLIWVEYLKICNKIMPSDKQL 126
FP+LENY++++ LLEYKLGN H IF+ LQ FN RSLL+W YLK CN ++ KQL
Sbjct: 67 FPYLENYYIDFALLEYKLGNVSMSHKIFQRGLQAFNQRSLLLWTSYLKFCNNVISHQKQL 126
Query: 127 FQKYAFAESYIGLHFFGAEFWHMYLNEIKLRCKTPQHYIRTLRKVIEIPLYEYSSFYDLW 186
F+KY AE Y+GLHFF EFW +YL +I RC + + Y LRK++EIPL+ +S FY LW
Sbjct: 127 FKKYETAEEYVGLHFFSGEFWDLYLEQISSRCTSSKKYWNVLRKILEIPLHSFSKFYALW 186
Query: 187 LQTIEDVKDLSQLRFYAEDKELWKKLKIDTQLNGRRGPHLQVAKKALKKVSKELFLVVQY 246
LQ I+D+ DL QL EL KKLKID +GR+GP+LQ AKK LKK++KE+++VVQY
Sbjct: 187 LQRIDDIMDLKQLSQLTSKDELLKKLKIDINYSGRKGPYLQDAKKKLKKITKEMYMVVQY 246
Query: 247 EVMEIYELFESKITKRYYMSAEELIPHQEIIVWTKYFEYIREKKISDLIKLQFQRSVLPL 306
+V+EIY +FESKI YY S E L+ EI W KY +Y + L L FQR++LPL
Sbjct: 247 QVLEIYSIFESKIYINYYTSPETLVSSDEIETWIKYLDYTITLQTDSLTHLNFQRALLPL 306
Query: 307 AHYDHIWLQYANYLIDDLNDFIGAKNVMLQALSYVHKRSRIVTMLSSVLIRLGQISELHQ 366
AHYD +W++Y+ +LI+ ND +GAKNV+L L + K++ I+ +L SV+ +L + L
Sbjct: 307 AHYDLVWIKYSKWLINSKNDLLGAKNVLLMGLKFSLKKTEIIKLLYSVICKLNEYVLLRN 366
Query: 367 LYDDISLAFSNDLTTSDDFEIFIDYIQFNLFLSSANSKSRFSGLTNNIEFSDNLLDVLMK 426
L + I ++S+++ DDFEIF DY+QF F ++ SR+S +N + L D + K
Sbjct: 367 LLEKIESSYSDNVENVDDFEIFWDYLQFKTFCQNSLYSSRYSDSQSNGLLNKELFDKVWK 426
Query: 427 KASKEDDHNRQYILLNMVNEMYERFPRALVETKVYQHIINSKWKSFLENPKFWYLYCSLV 486
+ S ++ + Q ILLN + + Y + VE ++Q II W+ +L+N FW YC L+
Sbjct: 427 RLSCKEKKSGQEILLNNLVQFYSKDTVEFVEKNIFQKIIEFGWEYYLQNGMFWNCYCRLI 486
Query: 487 WYDNNITYLKRRKHIINDIIPSA-AKYGKAVLSEVELFISNYLPDDMELFTEAYTNQ 542
++D + +YL +R++I+ I P K+ ++VL + F +Y P++M+ E +T +
Sbjct: 487 YFDTSRSYLDKRQYIVRKIWPQIDKKFAQSVLPSLTEFCESYFPEEMDTLEEMFTEE 543
>SPBC4B4.09 [A] KOG1258 mRNA processing protein
Length = 612
Score = 80.1 bits (196), Expect = 8e-15
Identities = 67/302 (22%), Positives = 138/302 (45%), Gaps = 30/302 (9%)
Query: 44 ISKSINKDLLQLVRSTYESLLYQFPFLENYHVEYGLLEYKLGNFGKFHAIFENALQYFNN 103
+ ++ +K + +RS Y+ L ++P L Y +Y E+ + I+E + +
Sbjct: 45 VGRNSSKQAINTLRSVYDRFLGKYPLLFGYWKKYADFEFFVAGAEASEHIYERGIAGIPH 104
Query: 104 RSLLIWVEYLKICNKIMPSDKQLFQKYAFAESYIGLHFFGAEFWHMYLNEIKLRCKTPQH 163
S+ +W Y + ++ + + + +GL F FW YL E + R + P +
Sbjct: 105 -SVDLWTNYCAFKMETNGDANEVRELFMQGANMVGLDFLSHPFWDKYL-EFEERQERPDN 162
Query: 164 YIRTLRKVIEIPLYEYSSFYDLWLQTIEDVKDLSQLRFYAEDKELWKKLKIDT------- 216
+ L ++I IPL++Y+ +++ ++Q + + + QL ++ ++ D
Sbjct: 163 VFQLLERLIHIPLHQYARYFERFVQ-VSQSQPIQQLL----PPDVLASIRADVTREPAKV 217
Query: 217 ------QLNGRRGPHLQVAKK---ALKKVSKELFLVVQYEVMEIYELFESKITKRYYMSA 267
Q+ RG L++ ++ + + ++F VQ E + + FES+I KR Y
Sbjct: 218 VSAGSKQITVERG-ELEIEREMRARIYNIHLQIFQKVQLETAKRW-TFESEI-KRPYFHV 274
Query: 268 EELIPHQEIIVWTKYFEYIREKKISDLIKLQFQRSVLPLAHYDHIWLQYANYLI---DDL 324
+EL +++ W KY ++ + I ++R ++ A YD W +YA ++ D L
Sbjct: 275 KEL-DEAQLVNWRKYLDFEEVEGDFQRICHLYERCLITCALYDEFWFRYARWMSAQPDHL 333
Query: 325 ND 326
ND
Sbjct: 334 ND 335
>YML046w [A] KOG1258 mRNA processing protein
Length = 629
Score = 70.5 bits (171), Expect = 6e-12
Identities = 64/330 (19%), Positives = 140/330 (42%), Gaps = 57/330 (17%)
Query: 56 VRSTYESLLYQ----FPFLENYHVEYGLLEYKLGNFGKFHAIFENALQYFNNRSLLIWVE 111
++ E++L+Q +P L + + +EY+L K A+ ++++F SL +W +
Sbjct: 69 IKLALETILWQILRKYPLLFGFWKRFATIEYQLFGLKKSIAVLATSVKWFPT-SLELWCD 127
Query: 112 YLKICNKIMPSDKQLFQ-KYAFAESYIGLHFFGAEFWHMYLNEIKLRCKTPQHYIRTLRK 170
YL + P++ + + A+ IG F FW ++ E ++ K + R
Sbjct: 128 YLNVLCVNNPNETDFIRNNFEIAKDLIGKQFLSHPFWDKFI-EFEVGQKNWHNVQRIYEY 186
Query: 171 VIEIPLYEYSSFYDLWLQTIEDVKDLSQLRFYAEDKELWKKLKIDTQLNGRRGPHLQVAK 230
+IE+PL++Y+ F+ + + + +K L ID L
Sbjct: 187 IIEVPLHQYARFF-------------TSYKKFLNEKNLKTTRNIDIVLR----------- 222
Query: 231 KALKKVSKELFLVVQYEVMEIYELFESKITKRYYMSAEELIPHQEIIVWTKYFEYIREKK 290
Q V EI++ FESKI + ++ + L + ++ W++Y +++ +
Sbjct: 223 ------------KTQTTVNEIWQ-FESKIKQPFFNLGQVL--NDDLENWSRYLKFVTDPS 267
Query: 291 IS---DLIKLQFQRSVLPLAHYDHIWLQYANYLIDDLNDFIGAKNVMLQALSYVHKRSRI 347
S + + F R ++P ++++ W+ Y +L KN+ + + +++++
Sbjct: 268 KSLDKEFVMSVFDRCLIPCLYHENTWMMYIKWLT--------KKNISDEVVVDIYQKANT 319
Query: 348 VTMLSSVLIRLGQISELHQLYDDISLAFSN 377
L +R + L + Y + F+N
Sbjct: 320 FLPLDFKTLRYDFLRFLKRKYRSNNTLFNN 349
>At1g04080 [A] KOG1258 mRNA processing protein
Length = 768
Score = 52.4 bits (124), Expect = 2e-06
Identities = 60/298 (20%), Positives = 124/298 (41%), Gaps = 32/298 (10%)
Query: 45 SKSINKDLLQLVRSTYESLLYQFPFLENYHVEYGLLEYKLGNFGKFHAIFENALQYFNNR 104
++ I +D + +R Y++ L +FP Y ++ E ++G K ++E A+
Sbjct: 110 TERIAQDNIAKIRKVYDAFLAEFPLCYGYWKKFADHEARVGAMDKVVEVYERAVLGV-TY 168
Query: 105 SLLIWVEYLKICNKIMPSDKQLFQKYAFAESYIGLHFFGAEFWHMYLNEIKLRCKTPQHY 164
S+ IW+ Y + + + + A Y+G F + W Y+ E + +
Sbjct: 169 SVDIWLHYCTFAINTYGDPETIRRLFERALVYVGTDFLSSPLWDKYI-EYEYMQQDWSRV 227
Query: 165 IRTLRKVIEIPLYEYSSFYDLWLQTIEDVKDLSQLRFYAEDKELWKKLKID--------- 215
+++E P+ ++ + + + + + LS+LR E + D
Sbjct: 228 ALIYTRILENPIQNLDRYFSSF-KELAETRPLSELRSAEESAAAAVAVAGDASESAASES 286
Query: 216 --------TQLNG--RRGPHLQVAKKALKKVSKELFLVVQYEVMEIYELFESKI------ 259
+Q++G + P L+ A + K+ + + ++ E FESKI
Sbjct: 287 GEKADEGRSQVDGSTEQSPKLESASSTEPEELKKYVGIREAMYIKSKE-FESKIIGYEMA 345
Query: 260 TKRYYMSAEELIPHQEIIVWTKYFEYI-REKKISDLIKLQFQRSVLPLAHYDHIWLQY 316
+R Y L E+ W Y ++I R+ + ++KL ++R V+ A+Y W++Y
Sbjct: 346 IRRPYFHVRPL-NVAELENWHNYLDFIERDGDFNKVVKL-YERCVVTCANYPEYWIRY 401
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.323 0.139 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,869,966
Number of Sequences: 60738
Number of extensions: 1360599
Number of successful extensions: 3510
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3499
Number of HSP's gapped (non-prelim): 8
length of query: 549
length of database: 30,389,216
effective HSP length: 111
effective length of query: 438
effective length of database: 23,647,298
effective search space: 10357516524
effective search space used: 10357516524
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)