ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3755 good P KOG2399 Inorganic ion transport and metabolism K+-dependent Na+:Ca2+ antiporter
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3755 1324417 1322366 -684
(684 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL206w [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 357 3e-98
SPAC3A12.06c [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 148 3e-35
CE08141 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 94 9e-19
CE00501 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 68 4e-11
Hs13376449 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 67 1e-10
Hs10190740 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 an... 65 4e-10
At5g17850 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 65 4e-10
Hs9966787 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 and... 63 1e-09
At5g17860 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 62 3e-09
At3g14070_2 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 61 6e-09
Hs17477322 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 an... 60 8e-09
Hs4759128 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 and... 60 1e-08
At1g08960 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 59 2e-08
At1g54110_2 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 59 2e-08
YJR106w [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 58 4e-08
CE26327 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 57 7e-08
7297547 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 and r... 56 2e-07
CE08140 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter 54 8e-07
CE16611 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 and r... 52 4e-06
7296864 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 and r... 51 7e-06
>YDL206w [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 762
Score = 357 bits (917), Expect = 3e-98
Identities = 251/773 (32%), Positives = 373/773 (47%), Gaps = 111/773 (14%)
Query: 6 RKLFVLTFYLGLTVIVGFAQLSD-EVFRYVFSPLSLLTCFIALGIITSDYLTPNLTVISQ 64
R L + FY+ +++G++ S+ + + + L+ CF LG++TSD LTP+L+ IS
Sbjct: 6 RWLITIAFYVSNVILIGYSLSSNGSISEFYLHSVVLIECFSLLGVVTSDCLTPSLSYISS 65
Query: 65 ELLFISDRISGMTLLALGNAIPDITSTYKAMKRGTTTLAIGESLGAIFFLLTVVIGSMLL 124
+ ISDR+SGMTLLALGNA+PDITSTY++MK G T+LAIGE G IFFLLTVVIG M
Sbjct: 66 NIFHISDRVSGMTLLALGNALPDITSTYQSMKSGVTSLAIGELFGGIFFLLTVVIGLMGC 125
Query: 125 VRNIKLSTPPESMPLNFGTEPDGLDIWDTIYYNRRQFLRDLFMFVFLILMNIFLLRDGKL 184
V I+ + TE + D + Y+R ++ D+ +F F++L++ L DG+L
Sbjct: 126 VATIQFQ---HDKSIETYTE-ESFD--QNLSYDRSNYILDVGIFTFMLLVSGTFLADGRL 179
Query: 185 AFWECFLMCVAYCVYATYLIISTRH----------------------------------- 209
FWEC +M + YC A YLI S ++
Sbjct: 180 YFWECIVMVLTYCCCAVYLIKSYKYPCEINDALEREVEIKKTVLANNHITVPNRFTLTTT 239
Query: 210 SDASNLDENIEPL-VITDHTIHEDT---------------NMERFLRTVQDXXXXXXXXX 253
SD ++ D+ I + + D I ED N+ RF + + +
Sbjct: 240 SDITSTDDGIRYVRPLGDTQIDEDNAISLDPTRLPSKSLDNISRFNQGIPERRDLIRRRI 299
Query: 254 XXXXXXNYTGWANLKLNDTLDLWEKGTIFNHEVDE--------------AISNVGSPNTR 299
+Y GW + L D L++WEK +FN+ V ++ G P R
Sbjct: 300 RGYLRSHYHGWVRMTLQDLLNIWEKQNLFNNTVKSLSLPSDDTHLFTKASLDEEGRPLIR 359
Query: 300 PMPRSNSETNFQH---LQNIPNISLNETS---PQDPSET--KSSTSLFSPQAQHLYHKSD 351
S +F L+N N + +T+ PQ+ +T TSLF Q K
Sbjct: 360 KRMNSLQPKDFYKYLSLRNGENSNALDTAISAPQNEYQTYYNEPTSLFLTVPQKKTSKKS 419
Query: 352 LEL---------NSPISDESLLRPKDRMETLRSAS--ADNIRLMTEQSSMLDDMSALNLQ 400
L N+ I ++ R ++ L S S DN L E+ ++ D + L+L
Sbjct: 420 LSCDRIPNLVRSNNIILNDEATRTQESTNALNSISDVIDNSLLQYERDDIILDRT-LSL- 477
Query: 401 LDNGISAEPEDQWESKLRVFRYLTDPNFATSNFELAIILITTPIVFFLTLIIPLHYSSMS 460
S + W S +++ YLTD + FE +L+TTP+ L L IP S
Sbjct: 478 ----CSTKSRTAWHS-FQLYNYLTDVSLEIGFFEFLSLLVTTPVSIILYLSIPSEISQTD 532
Query: 461 GSNFFKAAYYCQCAFSPFLTLLLVQREFNIWLFTLGLIVSCILLLMFFKWKLTVKEKYXX 520
Q SP + L+ F+ WL L L+++ +L++FK + T+ K+
Sbjct: 533 HDLPLSYLQNIQLIASPIILNQLITNNFSFWLLILSLVIA---ILLYFKTR-TIPNKFNS 588
Query: 521 XXXXXXXXXXXXXXXXXXXDI----LTNWAETFNLSEAILGLTVFAWGNSIGDLVSNVTF 576
I LT+W FN+SE ILGLT+F WGNSIGDLVSN+TF
Sbjct: 589 DIIFTVAFLLSLACLSKAVHIIVVTLTHWINVFNISETILGLTIFTWGNSIGDLVSNITF 648
Query: 577 TRIGVLEIALGSCFGSPLLYFLFGVGVDGMLVLLQREYPE-----GTAIWSRHIDFDVNR 631
+IGVLEIA+G+CFGSPLLYFLFGVG DG++++L + + + I HIDF V++
Sbjct: 649 VKIGVLEIAIGACFGSPLLYFLFGVGFDGIMIMLGDKTGKIVSGRDSNILMHHIDFKVDK 708
Query: 632 GLVFNCXXXXXXXXXXXXXXPLNNWKLDKRVGCLLLFLYGIITTINVYFELNK 684
L+ PLN+WK+DK++ LL LY ++T I+V+ E+++
Sbjct: 709 NLINTGVGILIAFLIFTVLIPLNDWKIDKKISIALLTLYIVVTCISVFLEVHQ 761
>SPAC3A12.06c [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 743
Score = 148 bits (373), Expect = 3e-35
Identities = 166/696 (23%), Positives = 282/696 (39%), Gaps = 124/696 (17%)
Query: 40 LLTCFIALGIITSDYLTPNLTVISQELLFISDRISGMTLLALGNAIPDITSTYKAMKRGT 99
L+ FI +GI SD+ + NL IS LL + D + G+T LALGN PDI ST+ A++ +
Sbjct: 119 LIFLFITIGISASDFFSTNLVTISW-LLQLPDSVVGVTFLALGNGSPDILSTFAAVRVNS 177
Query: 100 TTLAIGESLGAIFFLLTVVIGSMLLVRNIKLSTPPESMPLNFGTEPDGLDIWDTIYYNRR 159
+AIGE LG+ FF++ +V GS+ L++ K+ RR
Sbjct: 178 GGMAIGELLGSAFFIVAIVAGSVCLIKPFKIP--------------------------RR 211
Query: 160 QFLRDL-FMFVFLILMNIFLLRDGKLAFWECFLMCVAYCVYATYLIISTRHSDASNL-DE 217
FLRD+ F+ ++L+ +F+L DG L+ W+ +M + Y +Y ++ S + + DE
Sbjct: 212 HFLRDVAFLTGTILLVIMFVLHDGSLSIWQSLVMILYYLLYVLFVFFSGSSGVSVVITDE 271
Query: 218 NIEPLVI------------TDHTIHEDTNM--ERFLRTVQDXXXXXXXXXXXXX--XXNY 261
N P+ + +D D+ + E FL Q +Y
Sbjct: 272 NYLPVSLPVYSPVLNSFDDSDSYSSTDSELSEEAFLLPAQASRKTQKIHYINDNDPSNSY 331
Query: 262 TGWANLKLNDTLDLWEKGTIFNHEVDEAISNVGSPNTRPMP------RSNSETNFQHLQN 315
+ + + ++D + K ++ V +S P RP RS++E L++
Sbjct: 332 SSYQHSHVHDFI---HKNNTHSNRV---LSQSSGPIVRPSLLAALDFRSSNEEQHPGLRS 385
Query: 316 IPNISLNETS-PQDPSETKSSTSLFSPQAQHLYHKSDLELNSPISDESLLRPKDRMETLR 374
+ +++ + P + S S F P +N+P+S +
Sbjct: 386 LDPLNIQDGDLTMHPMHIRHSQSDFYPSG----------INTPVSGINYPN--------L 427
Query: 375 SASADNIRLMTEQSSMLDDMSALNLQLDNGISAEPEDQWESKLRVFRYLTDPNFATSN-F 433
SA+N S++ ++ D P S L + T NFA + +
Sbjct: 428 GFSANN-----SVQSLVSEIFRHPTHTDEDFPL-PSPSLSSLL----FPTLRNFAKKSWY 477
Query: 434 ELAIILITTPIVFFLTLII-----------PLHYSSMSGSNFFKAAY-----YCQCAFSP 477
E + ++ P V TL + P+++ +S N K + QC F P
Sbjct: 478 EKLMDVLAVPSVLIFTLALPVYQCPRLAVDPIYHMDVSNCNPSKPTWSRKLRLLQCVFVP 537
Query: 478 F-LTLLLVQREFNIWLFTLGLIVS--CILLLMFFKWKLTVKEK------YXXXXXXXXXX 528
F + ++++ + S CI L ++ T +EK +
Sbjct: 538 FAFVTFSITGGNRLYIYAASSVFSILCITALYYY----TDEEKPPKFLPWVSFIGFVLGI 593
Query: 529 XXXXXXXXXXXDILTNWAETFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLEIALGS 588
IL FNL+E+ILGLTVFA GNS+ DL++++ R G E+A+G
Sbjct: 594 IWISTIANEVVGILRALGVIFNLNESILGLTVFAAGNSLSDLIADIMIARSGFPEMAMGG 653
Query: 589 CFGSPLLYFLFGVGVDGMLVLLQREYPEGTAIWSRHIDFDVNRGLVFNCXXXXXXXXXXX 648
FG P L L G+G+ + + ++ L
Sbjct: 654 VFGGPTLNILIGIGISSFYSSISNHGNDSV--------IEIPHSLSITAYFLLACLLLLL 705
Query: 649 XXXPLNNWKLDKRVGCLLLFLYGIITTINVYFELNK 684
PLN +++++ +G LL LY + T+ N+ EL K
Sbjct: 706 IYVPLNRFRVNRVLGLLLFILYIVGTSTNIVVELLK 741
>CE08141 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 651
Score = 93.6 bits (231), Expect = 9e-19
Identities = 124/593 (20%), Positives = 231/593 (38%), Gaps = 86/593 (14%)
Query: 30 VFRYVFSPLSLLTCFIALGIITSDYLTPNLTVISQELLFISDRISGMTLLALGNAIPDIT 89
V + L L+ F+ + I D+ P ++ I L +S+ I+G+T LA GN PD+
Sbjct: 51 VILIILGILYLIILFVIMSSIADDFFCPAISGIVSHLR-MSESIAGVTFLAFGNGAPDVF 109
Query: 90 STYKAM---KRGTTTLAIGESLGAIFFLLTVVIGSMLLVRNIKLSTPPESMPLNFGTEPD 146
S+ ++ + LA+G+ G F+ TVV+ ++ ++ K++ P
Sbjct: 110 SSISSVLTTPKPKADLALGDLFGTSIFVTTVVLAIIIFTKSFKVAIIPT----------- 158
Query: 147 GLDIWDTIYYNRRQFLRDLFMFVFLILMNIF-LLRDGKLAFWECFLMCVAYCVYATYLII 205
LRDL ++ + +F L+ K+ W Y VY +II
Sbjct: 159 ---------------LRDLIFYMTTLAFIVFCFLKFDKIEVWMPATFLGIYGVYVVTVII 203
Query: 206 STRHSDASNLDENIEPLVITDHTIHEDTNMERFLRTVQDXXXXXXXXXXXXXXXNYTGWA 265
+ T + N+++ + ++D G
Sbjct: 204 LGIY-----------------RTHRKKRNLKKKNKELEDFLSRPSSAASTTPIF---GAM 243
Query: 266 NLKLNDTLDLWEKGTIFNHEV--DEAISNVGSPNTRPMPRSNSETNFQHLQNIPNISLNE 323
LK +T+ + +F+ + + I N+ N + +N+ N + I N+ +E
Sbjct: 244 KLK-EETISV---SALFHFMIGYSQFIKNLTRANLKRTTNNNNNDNKNEKRGIVNLGFSE 299
Query: 324 TSPQDPSETKSSTSLFSPQAQHLYHKSDLELNSPISDESLLRPKDRMETLRSASADNIRL 383
PSE K ST +F ++DLE ++D + R E A + +
Sbjct: 300 PY-SIPSERKIST-IFKQNT----FETDLESLESLADLDGSDDEGREEKFGYA---HHTV 350
Query: 384 MTEQSSMLDDMSALNLQLDNGISAEPEDQWESKLRVFRYLTD----PNFATSNFELAIIL 439
T D +S + +++ I W+ +F +L F+ N + I+
Sbjct: 351 FTSH----DQISLVASEIEE-IEITTWRSWDWVWDLFNHLKSWPSRDEFSEMNIFIKIVT 405
Query: 440 ITTPI-VFFLTLIIPLHYSSMSGSNFFKAAYYCQCAFSPFLTLLLVQREFN----IWLFT 494
+ + VFF L +P + S F + F+ F ++ + F+ +WL+
Sbjct: 406 VIKVVPVFFFKLTVPSNEMSWCKPLFILHCF-ASIQFALFSIQIITLKPFDGSPGLWLYG 464
Query: 495 LGLIVSCILLLMFFKWKLTVKEKYXXXXXXXXXXXXXXXXXXXXXD----ILTNWAETFN 550
LG ++ M+F L+ ++KY + ++T
Sbjct: 465 LGFSAILAMVAMYFL-PLSKEQKYYKEIYSYLGFLMSIAWIYATSNEIVSVVTMIGVVTG 523
Query: 551 LSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLEIALGSCFGSPLLYFLFGVGV 603
LS +LGLT+ AW N IGD+V+++ + G ++A+ + G PL L G G+
Sbjct: 524 LSMELLGLTIMAWSNCIGDIVADIAVVKQGYPKMAMAAAIGGPLFNLLIGFGL 576
>CE00501 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 590
Score = 68.2 bits (165), Expect = 4e-11
Identities = 51/188 (27%), Positives = 87/188 (46%), Gaps = 12/188 (6%)
Query: 435 LAIILITTPIVFFLTLIIPLHYSSMSGSNFFKAAYYCQCAFSPFLTLLLVQREFN----I 490
+A I + ++F LT IPL+ S S A+ C AF F ++ F+ +
Sbjct: 343 MAYINVVPNLLFKLT--IPLNEMSWSKPLTLLHAFTCP-AFLLFSIQFFLETPFSGSPGL 399
Query: 491 WLFTLGLIVSCILLLMFFKWKLTVKEKYXXXXXXXXXXXXXXX----XXXXXXDILTNWA 546
W++ L + + +L+M F +L+V+ KY +++T
Sbjct: 400 WVYGLAVSIVLAILIMVFT-ELSVQPKYYKEIYSYSGFIMSIAWIYLISSEVVNVVTMLG 458
Query: 547 ETFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLEIALGSCFGSPLLYFLFGVGVDGM 606
+S +LGLT+ AW NSIGDL+++V+ + G +A+ + G PL L G G+
Sbjct: 459 VVSRVSHEVLGLTILAWSNSIGDLIADVSVVKQGYPRMAMAAAIGGPLFNLLMGFGLPFT 518
Query: 607 LVLLQREY 614
+ LQ +Y
Sbjct: 519 IAKLQGKY 526
Score = 51.2 bits (121), Expect = 5e-06
Identities = 43/182 (23%), Positives = 77/182 (41%), Gaps = 29/182 (15%)
Query: 26 LSDEVFRYVFSPLSLLTCFIALGIITSDYLTPNLTVISQELLFISDRISGMTLLALGNAI 85
L EV + + +L FI + D+ +P+++ I L IS+ ++G+T +A GN
Sbjct: 2 LVKEVILIIVGVIYMLVLFIMVSSAADDFFSPSISSIVAHLR-ISESVAGVTFMAFGNGA 60
Query: 86 PDITSTYKAMKRGTT---TLAIGESLGAIFFLLTVVIGSMLLVRNIKLSTPPESMPLNFG 142
PD+ + ++ T LA+GE G F+ T+V+ +++L
Sbjct: 61 PDVFGSIASVLSSPTPKADLALGELFGGGLFVTTMVVSTIIL------------------ 102
Query: 143 TEPDGLDIWDTIYYNRRQFLRDLFMFVFLILMNIFLLRDGKLAFWECFLMCVAYCVYATY 202
T P ++++ TI R L L FL +F ++ W Y +Y
Sbjct: 103 TSPFDVEVFSTI----RDLLFYLVALSFLAFCFVFY---NRVTLWMPLTFLGLYLLYVIT 155
Query: 203 LI 204
+I
Sbjct: 156 VI 157
>Hs13376449 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 389
Score = 66.6 bits (161), Expect = 1e-10
Identities = 47/136 (34%), Positives = 67/136 (48%), Gaps = 21/136 (15%)
Query: 540 DILTNWAETFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLEIALGSCFGSPLLYFLF 599
+IL + F LS +LGLT+ AWGNSIGD S+ T R G +A +CFG + L
Sbjct: 247 NILRSLGVVFRLSNTVLGLTLLAWGNSIGDAFSDFTLARQGYPRMAFSACFGGIIFNILV 306
Query: 600 GVGVDGMLVLLQREY------PEGTAIWSRHIDFDVNRGLVFNCXXXXXXXXXXXXXXPL 653
GVG+ G L+ + R + P+G +W + + LVF+ PL
Sbjct: 307 GVGL-GCLLQISRSHTEVKLEPDGLLVWV--LAGALGLSLVFS-----------LVSVPL 352
Query: 654 NNWKLDKRVG-CLLLF 668
++L + G CLLLF
Sbjct: 353 QCFQLSRVYGFCLLLF 368
>Hs10190740 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 and related
proteins
Length = 593
Score = 64.7 bits (156), Expect = 4e-10
Identities = 37/136 (27%), Positives = 65/136 (47%), Gaps = 8/136 (5%)
Query: 548 TFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLEIALGSCFGSPLLYFLFGVGVDGML 607
T + + I+G+T A G S+ D ++++ R G+ ++A+ + GS + L G+G+ L
Sbjct: 453 TLGIPDVIMGITFLAAGTSVPDCMASLIVARQGMGDMAVSNSIGSNVFDILIGLGLPWAL 512
Query: 608 VLLQREYPEGTAIWSRHIDFDVNRGLVFNCXXXXXXXXXXXXXXPLNNWKLDKRVGCLLL 667
L +Y + SR GL+++ LN W+LDK++GC L
Sbjct: 513 QTLAVDYGSYIRLNSR--------GLIYSVGLLLASVFVTVFGVHLNKWQLDKKLGCGCL 564
Query: 668 FLYGIITTINVYFELN 683
LYG+ ++ E N
Sbjct: 565 LLYGVFLCFSIMTEFN 580
>At5g17850 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 559
Score = 64.7 bits (156), Expect = 4e-10
Identities = 47/188 (25%), Positives = 83/188 (44%), Gaps = 27/188 (14%)
Query: 38 LSLLTCFIALGIITSDYLTPNLTVISQELLFISDRISGMTLLALGNAIPDITST---YKA 94
L LL F LG S+Y +L +S+ LL +S ++G+TLL+LGN PD+ ++ +
Sbjct: 97 LWLLLLFYLLGHTASEYFCSSLESLSK-LLNLSPTVAGVTLLSLGNGAPDLFASLVSFMG 155
Query: 95 MKRGTTTLAIGESLGAIFFLLTVVIGSMLLVRNIKLSTPPESMPLNFGTEPDGLDIWDTI 154
+GT + + +G F+ VV+G + + + + +
Sbjct: 156 ESKGTYDVGLNTVVGGSGFVTCVVVGIISISLHKR-----------------------RV 192
Query: 155 YYNRRQFLRDLFMFVFLILMNIFLLRDGKLAFWECFLMCVAYCVYATYLIISTRHSDASN 214
R F+RD+ F I +L GK+ FW C Y VY ++++S R
Sbjct: 193 RIERAAFIRDICFFCAAIGSLALILVYGKINFWGALGFCSLYAVYVAFVVLSWRFGGDQG 252
Query: 215 LDENIEPL 222
+ ++E +
Sbjct: 253 AESDLESI 260
Score = 57.0 bits (136), Expect = 9e-08
Identities = 28/66 (42%), Positives = 41/66 (61%), Gaps = 3/66 (4%)
Query: 541 ILTNWAETFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLE---IALGSCFGSPLLYF 597
+LT+ F +S +ILGLTV AWGNSIGDL++N+T E +A+ C+ P+
Sbjct: 419 LLTSLGYIFGVSPSILGLTVLAWGNSIGDLITNLTMALHDGNEGAQVAVSGCYAGPIFNT 478
Query: 598 LFGVGV 603
LF +G+
Sbjct: 479 LFALGI 484
>Hs9966787 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 and related
proteins
Length = 661
Score = 63.2 bits (152), Expect = 1e-09
Identities = 42/166 (25%), Positives = 76/166 (45%), Gaps = 25/166 (15%)
Query: 38 LSLLTCFIALGIITSDYLTPNLTVISQELLFISDRISGMTLLALGNAIPDITSTYKAMKR 97
+ ++ FIAL I+ ++ P+LTVI+++L ISD ++G T +A G + P++ ++ +
Sbjct: 140 IGMIYMFIALAIVCDEFFVPSLTVITEKL-GISDDVAGATFMAAGGSAPELFTSLIGVFI 198
Query: 98 GTTTLAIGESLGAIFFLLTVVIGSMLLVRNIKLSTPPESMPLNFGTEPDGLDIWDTIYYN 157
+ + IG +G+ F + VIG L F E L W
Sbjct: 199 AHSNVGIGTIVGSAVFNILFVIGMCAL----------------FSREILNLTWW------ 236
Query: 158 RRQFLRDLFMFVFLILMNIFLLRDGKLAFWECFLMCVAYCVYATYL 203
RD+ ++ ++M I D + +WE L+ AY Y ++
Sbjct: 237 --PLFRDVSFYIVDLIMLIIFFLDNVIMWWESLLLLTAYFCYVVFM 280
>At5g17860 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 570
Score = 62.0 bits (149), Expect = 3e-09
Identities = 44/133 (33%), Positives = 63/133 (47%), Gaps = 11/133 (8%)
Query: 541 ILTNWAETFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIG---VLEIALGSCFGSPLLYF 597
+L + F +S ++LGLTV AWGNS+GDL++NVT G +IAL C+ PL
Sbjct: 427 LLISLGNIFGISPSVLGLTVLAWGNSLGDLIANVTVAFHGGNDGAQIALSGCYAGPLFNT 486
Query: 598 LFGVGVDGMLVLLQREYPEGTAIWSRHIDFDVNRGLVFNCXXXXXXXXXXXXXXPLNNWK 657
+ G+GV +++ EYP I S + L+ P +
Sbjct: 487 VIGLGVP-LVISSLAEYPGVYIIPS-------DNSLLETLGFLMVGLLWALVIMPKKKMR 538
Query: 658 LDKRVGCLLLFLY 670
LDK VG LL +Y
Sbjct: 539 LDKLVGGGLLAIY 551
Score = 55.5 bits (132), Expect = 3e-07
Identities = 43/169 (25%), Positives = 76/169 (44%), Gaps = 27/169 (15%)
Query: 40 LLTCFIALGIITSDYLTPNLTVISQELLFISDRISGMTLLALGNAIPDITSTYKAMKRGT 99
L F LG + Y P+L +S+ +L +S ++G+TLL+LGN PD+ S+ + R
Sbjct: 109 LFVLFYLLGDTAASYFCPSLDSLSK-VLKLSPTMAGVTLLSLGNGAPDLFSSVVSFTRSN 167
Query: 100 T-TLAIGESLGAIFFLLTVVIGSMLLVRNIKLSTPPESMPLNFGTEPDGLDIWDTIYYNR 158
+ LG FF+ + V+G++ ++ G+ +D R
Sbjct: 168 NGDFGLNSILGGAFFVSSFVVGTICVL---------------IGSRDVAID--------R 204
Query: 159 RQFLRD-LFMFVFLILMNIFLLRDGKLAFWECFLMCVAYCVYATYLIIS 206
F+RD +F+ V L + + + GK+ W Y +Y +L +S
Sbjct: 205 NSFIRDVVFLLVALCCLGLIIF-IGKVTIWVALCYLSIYLLYVGFLSVS 252
>At3g14070_2 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 609
Score = 60.8 bits (146), Expect = 6e-09
Identities = 39/143 (27%), Positives = 65/143 (45%), Gaps = 16/143 (11%)
Query: 541 ILTNWAETFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIG--VLEIALGSCFGSPLLYFL 598
+L + E + ++ +IL LTV AWGNS+GDLVSN+ T G ++IAL C+ P+ L
Sbjct: 467 LLVTFGEIYGINPSILALTVLAWGNSMGDLVSNIALTMNGGDGVQIALSGCYAGPMFNTL 526
Query: 599 FGVGVDGMLVLLQREYPEGTAIWSRHID---FDVNRGLVFNCXXXXXXXXXXXXXXPLNN 655
G+G+ + WS+ D ++ L + P N+
Sbjct: 527 VGLGMSMLF-----------GAWSKSPDTYMLPEDKSLFYTLGFLVLGLVWAMVILPRND 575
Query: 656 WKLDKRVGCLLLFLYGIITTINV 678
+ + +G L+ +Y I T +
Sbjct: 576 MQPSRTLGVGLIAIYLIFVTFRL 598
>Hs17477322 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 and related
proteins
Length = 660
Score = 60.5 bits (145), Expect = 8e-09
Identities = 37/136 (27%), Positives = 63/136 (46%), Gaps = 8/136 (5%)
Query: 548 TFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLEIALGSCFGSPLLYFLFGVGVDGML 607
T + + I+G+T A G S+ D ++++ R G+ ++A+ + GS + L G+GV L
Sbjct: 519 TLGIPDVIMGITFLAAGTSVPDCMASLIVARQGLGDMAVSNTIGSNVFDILVGLGVPWGL 578
Query: 608 VLLQREYPEGTAIWSRHIDFDVNRGLVFNCXXXXXXXXXXXXXXPLNNWKLDKRVGCLLL 667
+ Y I SR GLV++ LN W+LD+++G +L
Sbjct: 579 QTMVVNYGSTVKINSR--------GLVYSVVLLLGSVALTVLGIHLNKWRLDRKLGVYVL 630
Query: 668 FLYGIITTINVYFELN 683
LY I ++ E N
Sbjct: 631 VLYAIFLCFSIMIEFN 646
>Hs4759128 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 and related
proteins
Length = 1099
Score = 60.1 bits (144), Expect = 1e-08
Identities = 42/166 (25%), Positives = 74/166 (44%), Gaps = 25/166 (15%)
Query: 34 VFSPLSLLTCFIALGIITSDYLTPNLTVISQELLFISDRISGMTLLALGNAIPDITSTYK 93
V ++ F+AL I+ +Y P L VI+ +L IS+ ++G T +A G + P++ ++
Sbjct: 456 VLHVFGMMYVFVALAIVCDEYFVPALGVITDKLQ-ISEDVAGATFMAAGGSAPELFTSLI 514
Query: 94 AMKRGTTTLAIGESLGAIFFLLTVVIGSMLLVRNIKLSTPPESMPLNFGTEPDGLDIWDT 153
+ + + IG +G+ F + VIG+ L F E L W
Sbjct: 515 GVFISHSNVGIGTIVGSAVFNILFVIGTCSL----------------FSREILNLTWWP- 557
Query: 154 IYYNRRQFLRDLFMFVFLILMNIFLLRDGKLAFWECFLMCVAYCVY 199
RD+ ++ ++M I D +A+WE L+ +AY Y
Sbjct: 558 -------LFRDVSFYILDLIMLILFFLDSLIAWWESLLLLLAYAFY 596
>At1g08960 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 546
Score = 59.3 bits (142), Expect = 2e-08
Identities = 50/189 (26%), Positives = 73/189 (38%), Gaps = 17/189 (8%)
Query: 442 TPIVFFLTLIIPLHYSSMSGSNFFKAAYYCQCAFSPFLT------------LLLVQREFN 489
TP+ L L IP S S F+++A C F+ T L
Sbjct: 299 TPVSVLLNLTIPKP-SPSEWSRFYRSANIVFCPFALLYTCNSFVQLNHPISFLFPNTHLP 357
Query: 490 IWLFTLGLIVSCILLLMFFKWKLTVKEKYXXXXXXXXXXXXX--XXXXXXXXDILTNWAE 547
+WL L + S + L F K K + + L
Sbjct: 358 LWLVVL-FMTSSLAFLHFTVEKQPPKTEQLPVIVVAFIMSVFWISTIAGELLNCLAALGT 416
Query: 548 TFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLEIALGSCFGSPLLYFLFGVGVDGML 607
L A+LGLTV AWGNS+GDLV++V + G +A+ CF P+ L G+G ++
Sbjct: 417 LLKLPPALLGLTVLAWGNSVGDLVADVAVAKAGRPAMAMAGCFAGPMFNMLVGLG-SALV 475
Query: 608 VLLQREYPE 616
+ YP+
Sbjct: 476 MQTANVYPD 484
>At1g54110_2 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 610
Score = 58.9 bits (141), Expect = 2e-08
Identities = 40/143 (27%), Positives = 64/143 (43%), Gaps = 16/143 (11%)
Query: 541 ILTNWAETFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIG--VLEIALGSCFGSPLLYFL 598
+L + + ++ +ILGLTV AWGNS+GDLVSN+ + G ++IAL C+ P+ L
Sbjct: 468 LLVTFGGIYGINPSILGLTVLAWGNSMGDLVSNIALSMNGGDGVQIALSGCYAGPMFNTL 527
Query: 599 FGVGVDGMLVLLQREYPEGTAIWSRHID---FDVNRGLVFNCXXXXXXXXXXXXXXPLNN 655
G+G+ L WS+ + + L + P N
Sbjct: 528 VGLGMSMFL-----------GAWSKSPETYMIPEDNSLFYTLGFLIFGLIWSLVMLPRNE 576
Query: 656 WKLDKRVGCLLLFLYGIITTINV 678
+ +K +G L+ LY I T +
Sbjct: 577 MRPNKVMGIGLITLYLIFVTFRL 599
>YJR106w [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 725
Score = 58.2 bits (139), Expect = 4e-08
Identities = 29/86 (33%), Positives = 49/86 (56%), Gaps = 15/86 (17%)
Query: 540 DILTNWAETFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLE---------------I 584
+++ + + LS+AILGLT+FAWGNS+GDL+SN++ R+ + I
Sbjct: 551 EMMEIYQKILGLSKAILGLTIFAWGNSVGDLISNISMCRLYKTQTHYQDRVRLATKFFMI 610
Query: 585 ALGSCFGSPLLYFLFGVGVDGMLVLL 610
+ SC G +L + G+G G++ +L
Sbjct: 611 SCASCLGGVMLNSMGGIGFSGLVSML 636
>CE26327 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 668
Score = 57.4 bits (137), Expect = 7e-08
Identities = 30/108 (27%), Positives = 62/108 (56%), Gaps = 4/108 (3%)
Query: 27 SDEVFRYVFSPLSLLTCFIALGIITSDYLTPNLTVISQELLFISDRISGMTLLALGNAIP 86
S + + S + LL F+ + ++ D+ +P++ I + L IS+ I+G+T LA GN P
Sbjct: 98 SIRIILIIISIIYLLFLFVVMTVVADDFFSPSIAGIVRHLK-ISESIAGVTFLAFGNGAP 156
Query: 87 DITSTYKAM---KRGTTTLAIGESLGAIFFLLTVVIGSMLLVRNIKLS 131
D+ + ++ + LAIG+ +G F+ TVV+ +++L ++ +++
Sbjct: 157 DVFGSIASVITTPKPKADLAIGDIIGGGIFVTTVVLSAIILTKSFRIA 204
Score = 56.2 bits (134), Expect = 2e-07
Identities = 46/184 (25%), Positives = 79/184 (42%), Gaps = 12/184 (6%)
Query: 425 DPNFATSNFELAIILITTPIVFFLTLIIPLHYSSMSGSNFFKAAYYCQCAFSPFLTLLLV 484
D + + F I ++ FFL L IP + S +C + F +
Sbjct: 392 DEFYEMNYFSRFITVVAVIPTFFLKLTIPSNEMPWS-KPILIIHCFCSIQLALFAVQISA 450
Query: 485 QREFN----IWLFTLGLIVSCILLLMFFKWK-LTVKEKYXXXXXXXXXXXXXXX----XX 535
+ F+ +WL+ GL++S IL L+ ++ L ++KY
Sbjct: 451 KSPFHGSPGLWLY--GLLISIILSLLALRFTPLDREQKYYREVYSYLGFLMSIAWIYTIS 508
Query: 536 XXXXDILTNWAETFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLEIALGSCFGSPLL 595
+++T +S+ ILGLT+ AW N IGD+VS+V + G ++A+ + G PL
Sbjct: 509 SEIINVITMIGVATGVSQEILGLTIMAWSNCIGDIVSDVAVVKQGFPKMAMAAAIGGPLF 568
Query: 596 YFLF 599
L+
Sbjct: 569 SKLY 572
>7297547 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 and related
proteins
Length = 856
Score = 56.2 bits (134), Expect = 2e-07
Identities = 38/166 (22%), Positives = 73/166 (43%), Gaps = 25/166 (15%)
Query: 38 LSLLTCFIALGIITSDYLTPNLTVISQELLFISDRISGMTLLALGNAIPDITSTYKAMKR 97
+ ++ F+AL I+ ++ P+L VI ++L I+D ++G T +A G + P++ ++ +
Sbjct: 338 IGVIYMFVALAIVCDEFFVPSLDVIIEKL-GITDDVAGATFMAAGGSAPELFTSVIGVFV 396
Query: 98 GTTTLAIGESLGAIFFLLTVVIGSMLLVRNIKLSTPPESMPLNFGTEPDGLDIWDTIYYN 157
+ IG +G+ F + VIG L LS L W
Sbjct: 397 SFDDVGIGTIVGSAVFNILFVIGMCALFSKTVLS----------------LTWW------ 434
Query: 158 RRQFLRDLFMFVFLILMNIFLLRDGKLAFWECFLMCVAYCVYATYL 203
RD + +L+ I+ RD ++ +WE ++ Y Y ++
Sbjct: 435 --PLFRDCSFYSISLLVLIYFFRDNRIFWWEALILFTIYIGYVAFM 478
>CE08140 [P] KOG2399 K+-dependent Na+:Ca2+ antiporter
Length = 703
Score = 53.9 bits (128), Expect = 8e-07
Identities = 43/184 (23%), Positives = 78/184 (42%), Gaps = 9/184 (4%)
Query: 428 FATSNFELAIILITTPIV-FFLTLIIPLHYSSMSGSNFFKAAYYCQCAFSPFLTLLLVQR 486
F+ N + II+I I FF+ + IP + S F + F+ F ++ +
Sbjct: 446 FSEMNIFVKIIMIIRIIPSFFMKMTIPSNEMSWC-KPLFLLHCFASIQFALFSIQIINLK 504
Query: 487 EFN----IWLFTLGLIVSCILLLMFF---KWKLTVKEKYXXXXXXXXXXXXXXXXXXXXX 539
F+ +WL+ L + LL + F +L ++
Sbjct: 505 PFDGSPGLWLYGLAFSATIALLALCFLPLNKELKYHKEVYSYLGFLMSIAWIYATANEII 564
Query: 540 DILTNWAETFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLEIALGSCFGSPLLYFLF 599
++T LS+ +LGLTV +W + IGD+V+++ + G ++A+ + G PL L
Sbjct: 565 SVMTMVGVVTGLSQELLGLTVMSWSDCIGDIVADIAVIKQGYPKMAMAAAIGGPLFNLLI 624
Query: 600 GVGV 603
G G+
Sbjct: 625 GFGL 628
Score = 52.4 bits (124), Expect = 2e-06
Identities = 30/105 (28%), Positives = 55/105 (51%), Gaps = 4/105 (3%)
Query: 40 LLTCFIALGIITSDYLTPNLTVISQELLFISDRISGMTLLALGNAIPDI---TSTYKAMK 96
L+ F+ + I D+ P+++ I L +S+ I+G+T LA GN PD+ S+ +
Sbjct: 112 LIFLFVVMSTIADDFFCPSISGIVTHLK-MSESIAGVTFLAFGNGAPDVFGSISSVLSTP 170
Query: 97 RGTTTLAIGESLGAIFFLLTVVIGSMLLVRNIKLSTPPESMPLNF 141
+ LA+G+ G F+ TVV+ ++ ++ K++ P L F
Sbjct: 171 KPKAALALGDLFGTSIFVTTVVLAIIIFTKSFKVAIIPTLRDLIF 215
>CE16611 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 and related
proteins
Length = 591
Score = 51.6 bits (122), Expect = 4e-06
Identities = 35/128 (27%), Positives = 60/128 (46%), Gaps = 12/128 (9%)
Query: 547 ETFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLEIALGSCFGSPLLYFLFGVGVDGM 606
ETF + I+GLT+ A G SI DL+++V R G+ ++A+ S GS L G+ + +
Sbjct: 449 ETFGIPTEIMGLTILAAGTSIPDLITSVIVARKGLGDMAVSSSIGSNLFDVCVGLPIPWL 508
Query: 607 ----LVLLQREYPEGTAIWSRHIDFDVNRGLVFNCXXXXXXXXXXXXXXPLNNWKLDKRV 662
+ + + E + ++ S GLV + ++ WK+DK
Sbjct: 509 IHFAIGVFKSEPTQNISVTS--------NGLVCSLGLLFAMLIVLLTCVAISRWKMDKFF 560
Query: 663 GCLLLFLY 670
G L++F Y
Sbjct: 561 GLLMIFSY 568
>7296864 [PT] KOG1307 K+-dependent Ca2+/Na+ exchanger NCKX1 and related
proteins
Length = 658
Score = 50.8 bits (120), Expect = 7e-06
Identities = 35/154 (22%), Positives = 68/154 (43%), Gaps = 14/154 (9%)
Query: 541 ILTNWAETFNLSEAILGLTVFAWGNSIGDLVSNVTFTRIGVLEIALGSCFGSPLLYFLFG 600
++T T + + ++GLT A G S+ D +S++ + G ++A+ + GS + L
Sbjct: 496 MITVIGSTLAIPDTVMGLTFVAAGVSVPDALSSIAVIKEGFGDMAVSNAIGSNVFDILVC 555
Query: 601 VGVDGMLV-----------LLQREYPEGTAIWSRHIDFDVNRGLVFNCXXXXXXXXXXXX 649
+G+ + ++ + + ++S+ F GL ++
Sbjct: 556 LGLPWFIQTAIIKPGSHVNVISKGAISLSCLFSKQFRFS---GLAYSTLSLFSTVVFLIL 612
Query: 650 XXPLNNWKLDKRVGCLLLFLYGIITTINVYFELN 683
LN WKLDKR+G +L+ Y T+ +ELN
Sbjct: 613 STHLNGWKLDKRLGIILMVWYLFFITLASLYELN 646
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.325 0.140 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,838,874
Number of Sequences: 60738
Number of extensions: 1466928
Number of successful extensions: 5364
Number of sequences better than 1.0e-05: 20
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 5281
Number of HSP's gapped (non-prelim): 79
length of query: 684
length of database: 30,389,216
effective HSP length: 113
effective length of query: 571
effective length of database: 23,525,822
effective search space: 13433244362
effective search space used: 13433244362
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)