ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3822 suspect: LH U KOG2273 Intracellular trafficking, secretion, and vesicular transport Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3822 1349181 1352126 982
(982 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPR097w [U] KOG2273 Membrane coat complex Retromer subunit VPS5/... 761 0.0
SPCC1450.12 [U] KOG2273 Membrane coat complex Retromer subunit V... 149 3e-35
>YPR097w [U] KOG2273 Membrane coat complex Retromer subunit VPS5/SNX1 Sorting
nexins and related PX domain-containing proteins
Length = 1073
Score = 761 bits (1966), Expect = 0.0
Identities = 436/1085 (40%), Positives = 626/1085 (57%), Gaps = 136/1085 (12%)
Query: 5 LSSVEEHYLKRELLRIELNTEFEKLNDINALRKFGCPFSDEDPKSKGKAXXXXXXXXXXX 64
L+ EEHYLKRELLR +L+ E KLND ALRKFG PFS DP +
Sbjct: 9 LNPTEEHYLKRELLRCQLDYEIGKLNDQFALRKFGYPFSPNDPTAPQPIS---------- 58
Query: 65 XXXXXXTLNSDDEV----KQKFVGEFPVLSHFLQNFVMTFPLLSKELVKDPSFWQGKVQV 120
N+D K F +P+LS+ LQ F+ TFPLLS L+ D FWQ KVQV
Sbjct: 59 --------NNDSSPVLGGKGHFSVNYPMLSYVLQEFISTFPLLSTNLLVDEKFWQSKVQV 110
Query: 121 FFEHFMSLPFSSSFDREELTKRRKMALKLSKVILLFYNSGIGVSKERQYYEQEKYHLTK- 179
FFEHFMSL FS S+DREE +KR+K++ KLSKVILL +NSG+G +E+ YY ++K+ L
Sbjct: 111 FFEHFMSLGFSESYDREEASKRKKVSKKLSKVILLLFNSGVGSFQEQAYYNEDKFVLQSG 170
Query: 180 DAEKATKLNKFTMPSKDNLQYLLTNEPIYFNGIDINVIAVVEHRLL-------------- 225
A K + + KF MP+++NL+ LLTNE ++ NG D+N+I+V
Sbjct: 171 QARKRSNIEKFAMPTRENLENLLTNESVFINGWDVNIISVFNKNSRKCTESVDNDKSSKS 230
Query: 226 FPDDKVKPKPAKSVAN--KWMKSNLGFD---------------FSKLSL-IDSGSSK--R 265
P K KS A+ KWMK+ FSKLSL + S SK R
Sbjct: 231 TPTSSPKSHAIKSFASTSKWMKNAFNNTINSTINSMPESSASLFSKLSLGVPSTKSKQSR 290
Query: 266 HQSYFMIRVRKEDP----------------ATTVFVYKTYEDFKRLAKDLKTGYPGKKLP 309
YF+I+++K+D A +V +TY DFK+L+ DLK+ +PGKK P
Sbjct: 291 KHHYFLIKIKKQDDDDQDNSNEENSNLDHHAGYFYVTRTYSDFKKLSHDLKSEFPGKKCP 350
Query: 310 QLPHKN-KIQASIKQDGV------------DVTETLESITQQAKAXXXXXXXXXXXXXXX 356
+LPH+N K+ + I + V + T ++ Q A
Sbjct: 351 RLPHRNKKVTSMITKTEVLHNGQTKSAAREKIVNTFDTDLQSASESDNSSFLQTTNELSA 410
Query: 357 XXXXXXXXXXXXNTPNI----------------------------------NKNRTLPRE 382
NI +K TL E
Sbjct: 411 TETVLTEKETETLRKNILNEIKEEDNIDEDEYEEEGEGEESDFDEYKDASDSKINTLVGE 470
Query: 383 RMRTSLRQYLRTLCQEKEVATGTPLKKFLFSKSERLEDAELNSAVKNDMKNRELVDLTNI 442
+MRTSLRQYLRTLC++ EV+ + +++F S L+ ++N + +D++NR L+D++N+
Sbjct: 471 KMRTSLRQYLRTLCKDAEVSQSSSIRRFFLS-GPNLDIKDINPKIADDIRNRALIDVSNL 529
Query: 443 EHQIKFQKMAFERSLKLQDSLKEMKTSXXXXXXXXXXXXKEFKEKRDPYELSPILRDFFD 502
E+QI+FQ+MA E+SLKLQDS+K+ KTS E K+ +LSP+L+DF +
Sbjct: 530 ENQIRFQQMALEKSLKLQDSMKDFKTSLLKDEKYLMSLLVEIKDNTKVEDLSPLLQDFVE 589
Query: 503 WCKIYMSATLYQMFLGDDSGYELYKQVRRLHKLLPYTIMIQILRFTNPIAIMKGMMDLFM 562
WCKIY+S+ +YQMFLG+D+ YELY Q+RRLHKL+PYT+M QI++FTNPIAIM+GM++LFM
Sbjct: 590 WCKIYISSMIYQMFLGNDNSYELYTQIRRLHKLMPYTVMGQIMKFTNPIAIMRGMIELFM 649
Query: 563 AQPFGGQSLLQTMFSTILTDDLKGQDKIIEELETVIKTNHQYGTEICRFFKKVIFENE-- 620
AQPFGG SLLQTMFSTILTDDLK Q I+ELE I + + + K +F N+
Sbjct: 650 AQPFGGHSLLQTMFSTILTDDLKTQKVAIKELERKIAEMDPGASVVTKCLKDFVFNNDTK 709
Query: 621 ---DSSLLDMDAVHSDVKLMSMPISVIITMRGTEMEHVSSDALAELMESYTYWKNEQENS 677
D+ L MDAV+++ + M+MP+ +I+ M+ + + +A L+ESY+ WK ++E++
Sbjct: 710 DEHDTKLFTMDAVNAESESMNMPVPLIVLMKSAAANLIPDEVVAGLIESYSSWKLQKEDT 769
Query: 678 IALVHVRSNDSLSASVGVYFTRIKEYLQLLIQERDKRLMKQLWEDPELSQLIKSMVTLFY 737
AL +V S D G+YFT +K+ QL I+E DK+LM+QLW+DPEL+Q++K++VT+ Y
Sbjct: 770 DAL-NVTSED----QSGIYFTHVKDLWQLYIKEHDKQLMRQLWQDPELTQMLKAIVTMIY 824
Query: 738 EPMIKVFKVARMDLAFRNFEKFMNDLIYLLDNVINGNKGSVTITNIVDEINELINKHETS 797
EPM+K+FKVARMD+A +NFEKFM DLI L+D+VING G T ++V+EI+ L+ KH+ +
Sbjct: 825 EPMVKIFKVARMDVALKNFEKFMGDLIRLVDDVINGQLGVSTQFDVVEEIHNLVTKHQDA 884
Query: 798 FYQFAHDIYVNDTQGIFEGFITWICSIINFLQRSKFGKVEDAIDLNVLLQKSDVDADI-- 855
F++F HD+Y+ND++GIFEGFITWI +I+ FLQ+SKFG + ID N L+ + D+D D+
Sbjct: 885 FFEFIHDVYLNDSEGIFEGFITWITTIVKFLQKSKFGGPSERIDFNKLICRDDIDIDVKL 944
Query: 856 VKEQLQQVIDKRLESRKLYSELVXXXXXXXXXXXXXXXXXVNNLLDRQWKNLNHMVIPXX 915
+K Q+ V++K++ +RK+Y +L+ +L + W ++N +V+P
Sbjct: 945 LKVQVNNVLNKKIGARKIYKKLLDLKVKQGTKQNNKHAA---GILQKNWSDINSLVMPSS 1001
Query: 916 XXXXXXXXXXXXXXXXXIQDYEYLQNDEADELELKYKAILDKEVPVSEIEKFLETTFRPK 975
DY++L + ELE +YK +L+ V SEI+K F +
Sbjct: 1002 SGSFGLGDGDLVDLDLDTGDYDFLHKENEVELEKQYKDLLNLVVDESEIDKLRSQVFAQE 1061
Query: 976 LREVL 980
L+ L
Sbjct: 1062 LKNYL 1066
>SPCC1450.12 [U] KOG2273 Membrane coat complex Retromer subunit VPS5/SNX1
Sorting nexins and related PX domain-containing proteins
Length = 821
Score = 149 bits (375), Expect = 3e-35
Identities = 135/597 (22%), Positives = 235/597 (38%), Gaps = 122/597 (20%)
Query: 4 ELSSVEEHYLKRELLRIELNTEFEKLNDINALRKFGCPFSDEDPKSKGKAXXXXXXXXXX 63
E+ +++ HYLK+EL + + E +++ L G PF + KGK+
Sbjct: 8 EIDALKRHYLKKELFSLLIADELNFVSEPTNLDHLGSPFVE-----KGKSVIPYEKS--- 59
Query: 64 XXXXXXXTLNSDDEVKQKFVGEFPVLSHFLQNFVMTFPLLSKELVKDPSFWQGKVQVFFE 123
+ PVL + F++TFP L + W + +
Sbjct: 60 ---------------------QIPVLRFIFKRFILTFPFLDPD--SQNQLWNVNFRNLLK 96
Query: 124 HFMSLPFSSSFDREELTKRRKMALKLSKVILLFYNSGIGVSKERQYYEQEKYHLTKDAEK 183
S D + T K LK ++ L + +H +D EK
Sbjct: 97 SLSKSNVSVLPDDSDATHLHKWLLKFQNMLTLLMSRA--------------FHSIQD-EK 141
Query: 184 ATKLNKFTMPS--KDNLQYLLTNEPIYFNGIDINVIAVVEHRLLFPDDKVKPKPAKSVAN 241
+ P KD + L +P N ++ V+ V +S
Sbjct: 142 NINIELLDTPKLEKDLHKSELQQKP---NILEAYVLGV----------------RQSTQA 182
Query: 242 KWMKSNLGFDFSKLSLIDSGSSKRHQSYFMIRVRKEDPATTVFVYKTYEDFKRLAKDLKT 301
K +++ +++ +I+V E+ T F+ + Y DF L
Sbjct: 183 KMLRTKRVYEY------------------LIKVSYEE--NTFFIARKYSDFSHFHHLLLK 222
Query: 302 GYPGKKLPQLPHKNKIQASIKQDGVDVTETLESITQQAKAXXXXXXXXXXXXXXXXXXXX 361
YP +P+LP K+ + L S +
Sbjct: 223 SYPNAYVPRLPPKDDHDTYLNSSEDSTLSPLPSRSSDT---------------------- 260
Query: 362 XXXXXXXNTPNINKNRTLPRERMRTSLRQYLRTLCQEKEVATGTPLKKFLFSKSERLEDA 421
N P + L +ERMR LRQY++ + + E+ + FL L +
Sbjct: 261 -------NDPQSDSQHVLRQERMRIELRQYIKNILNDDELHLTEEVLSFLTDDPVTLTAS 313
Query: 422 ELNSAVKNDMKNRELVDLTNIEHQIKFQKMAFERSLKLQDSLKEMKTSXXXXXXXXXXXX 481
EL D+ R+ +D+ Q KF ++A +R+ +L ++E K S
Sbjct: 314 ELT-----DINFRQELDVIRQLEQQKFVEIASQRAKELDVYMEEFKRSLTENNGFTTLFT 368
Query: 482 KEFKEKRDPYELSPILRDFFDWCKIYMSATLYQMFLGDDSGYELYKQVRRLHKLLPYTIM 541
E KEK ELSP L+ +W ++ +++T+Y F+G + E Y Q++++H+L PYT++
Sbjct: 369 -ELKEKNSISELSPSLQKTIEWARVNLASTIYDTFIGKEKSLETYLQIKKMHQLFPYTVI 427
Query: 542 IQILRFTNPIAIMKGMMDLFMAQPFGGQSLLQTMFSTILTDDLKGQDKIIEELETVI 598
I+RF+NP+++MK ++D +AQPFG +SL Q + S L ++++ K+I E I
Sbjct: 428 KNIMRFSNPLSVMKRILDTLLAQPFGMKSLFQRLLSISLNENVRAIHKLISRYEARI 484
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.136 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 51,088,092
Number of Sequences: 60738
Number of extensions: 2080806
Number of successful extensions: 5989
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 5970
Number of HSP's gapped (non-prelim): 8
length of query: 982
length of database: 30,389,216
effective HSP length: 116
effective length of query: 866
effective length of database: 23,343,608
effective search space: 20215564528
effective search space used: 20215564528
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)