ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4015 good P KOG1485 Inorganic ion transport and metabolism Mitochondrial Fe2+ transporter MMT1 and related transporters (cation diffusion facilitator superfamily)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII4015 1413160 1411622 -513
(513 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR177w [P] KOG1485 Mitochondrial Fe2+ transporter MMT1 and rela... 499 e-141
YPL224c [P] KOG1485 Mitochondrial Fe2+ transporter MMT1 and rela... 414 e-115
SPCC1020.03 [P] KOG1485 Mitochondrial Fe2+ transporter MMT1 and ... 177 4e-44
At2g47830 [P] KOG1485 Mitochondrial Fe2+ transporter MMT1 and re... 133 7e-31
CE16617 [P] KOG1482 Zn2+ transporter 56 1e-07
YOR316c [P] KOG1483 Zn2+ transporter ZNT1 and related Cd2+/Zn2+ ... 54 7e-07
CE03664 [P] KOG1482 Zn2+ transporter 51 4e-06
>YMR177w [P] KOG1485 Mitochondrial Fe2+ transporter MMT1 and related
transporters (cation diffusion facilitator superfamily)
Length = 510
Score = 499 bits (1285), Expect = e-141
Identities = 253/448 (56%), Positives = 322/448 (71%), Gaps = 26/448 (5%)
Query: 31 HSFAGLRAKEASPENITEPPKKVDSSANDLSKDGT---NAQEKLEDNPHFAKLAEDFSSH 87
H G++ +++S N + P KV N+L + A ++LE NP + KLAE F+SH
Sbjct: 30 HISTGVKVEKSSIIN-QKDPNKVRVEINELKRQAEIEKAAIKELEKNPQYQKLAEAFNSH 88
Query: 88 DHIHLQESETEENDSFKLGSML-------------------TRTPQHANHAHSHGLGNPM 128
DH+HL+ESETE+ND LG++ + T H H HSH NP+
Sbjct: 89 DHVHLRESETEQNDIISLGTIRDYKSSKCEQADKPSSLNLHSHTHSHG-HTHSHAAHNPL 147
Query: 129 LVLSREQFKNNPGVRITWIGLLTNVGLAVGKFTGGVVFHSQALIADSVHAISDLVSDFLT 188
LVLS EQ + N GVRITW+GL NVG+A+GKF GG+VFHSQAL AD++HAISD+VSD LT
Sbjct: 148 LVLSTEQIRKNAGVRITWVGLGVNVGIAIGKFFGGIVFHSQALFADAIHAISDMVSDLLT 207
Query: 189 LFSVGLATRKPTNDYPYGYGKVETIGSLAVSSILTTAGLSIGWSSLCAIAGPIVPHAILE 248
L SVGLA KPT DYPYGYGK+ET+GSLAVS+IL AG+SIGWSSLCA+ GP++PH I++
Sbjct: 208 LLSVGLAANKPTADYPYGYGKIETVGSLAVSTILAMAGISIGWSSLCALVGPVIPHTIID 267
Query: 249 FFAXXXXXXXXXXXXXX--TDINAAWIAGGSIILKEWIYKATKKVALETKSNVLMANAWH 306
TDINAAWIA SI KEWI++AT+K+A+ T SNVLMANAWH
Sbjct: 268 TIGNLGHAHTYSEDIIEDVTDINAAWIAAASIAAKEWIFRATRKIAINTNSNVLMANAWH 327
Query: 307 HRVDSLTSLVALVTISSGYLFNIQSLDAVGGLIVSGLVVKAGTQGMVASIKELIDKSLPP 366
HRVDSLTSLVALV IS+GYL NIQSLD +GGLIVSGL++KAG +GM +IKELID+S+
Sbjct: 328 HRVDSLTSLVALVAISTGYLVNIQSLDTIGGLIVSGLIIKAGGEGMCIAIKELIDQSVSR 387
Query: 367 TDSRYVEVETMTKEVLSKLISNNNSKKPYRIVDLTVMTSGPNMHANMVLEAPLQRWDNIL 426
D RY+E+ET+ K+ L+KLISNNNS+KPY + +LT+++SGPN+ ++ LE PLQ+W NIL
Sbjct: 388 DDPRYLEIETLVKDTLNKLISNNNSQKPYGLKELTLLSSGPNLRGHLTLEVPLQKWGNIL 447
Query: 427 SVSEFEIVTDHLRSVLYKNIPSLRRLNI 454
V+EFEIVT HLR+VL + +LRRL+I
Sbjct: 448 GVNEFEIVTHHLRNVLTNEVSNLRRLDI 475
>YPL224c [P] KOG1485 Mitochondrial Fe2+ transporter MMT1 and related
transporters (cation diffusion facilitator superfamily)
Length = 449
Score = 414 bits (1064), Expect = e-115
Identities = 208/409 (50%), Positives = 287/409 (69%), Gaps = 10/409 (2%)
Query: 46 ITEPPKKVDSSANDLSKDGTNAQEKLEDNPHFAKLAEDFSSHDHIHLQESETEENDSFKL 105
I+ P++ S+ L+K+G ++ + +L SS + + +++E+E K
Sbjct: 41 ISANPRRCLHSSKLLNKEG-------QEEGYNEQLISKMSSQNGSNSRQNESEGKKEGKA 93
Query: 106 GSMLTRTPQHANHAHSHGLGNPMLVLSREQFKNNPGVRITWIGLLTNVGLAVGKFTGGVV 165
S+ + +H+H+H NP+L L+ +Q K NPGVRITWIGL +NVG+AVGKF GG+
Sbjct: 94 SSVKSLLQHTHSHSHTHMHDNPLLSLNVQQIKKNPGVRITWIGLASNVGMAVGKFVGGIT 153
Query: 166 FHSQALIADSVHAISDLVSDFLTLFSVGLATRKPTNDYPYGYGKVETIGSLAVSSILTTA 225
FHSQAL+ADSVHA+SDLVSDFLTLFSV A+RKPT++YPYGYGKVET+GSLAVS+IL A
Sbjct: 154 FHSQALLADSVHALSDLVSDFLTLFSVQYASRKPTSEYPYGYGKVETVGSLAVSTILAMA 213
Query: 226 GLSIGWSSLCAIAGPIVPHAILEFFA---XXXXXXXXXXXXXXTDINAAWIAGGSIILKE 282
G+SIGWSSLCAI GP++PHAILE A T++NA WIA GSI++KE
Sbjct: 214 GISIGWSSLCAIVGPVIPHAILESMAGLIGETHSHSQSLTQQATNVNAVWIAAGSILVKE 273
Query: 283 WIYKATKKVALETKSNVLMANAWHHRVDSLTSLVALVTISSGYLFNIQSLDAVGGLIVSG 342
W+++ATKKVA++T SNVLMANAWHHRVDSLTSLVALV I+S Y FNIQSLD +GGL+VSG
Sbjct: 274 WVFQATKKVAIQTNSNVLMANAWHHRVDSLTSLVALVAITSSYFFNIQSLDNLGGLVVSG 333
Query: 343 LVVKAGTQGMVASIKELIDKSLPPTDSRYVEVETMTKEVLSKLISNNNSKKPYRIVDLTV 402
L++K G QG+++S+KEL+D+S+PPTD RY+E+E++ K+ + L ++ + K+ + DLT+
Sbjct: 334 LIIKTGGQGILSSLKELVDQSIPPTDPRYLEIESVIKDSIGSLKTDLDLKQSLHVRDLTI 393
Query: 403 MTSGPNMHANMVLEAPLQRWDNILSVSEFEIVTDHLRSVLYKNIPSLRR 451
+ SGPN+ A LE P+ + + E +R L +P++ R
Sbjct: 394 LASGPNLRATTTLEVPVLHSGQEVGIRFLENAISTIREDLRMKVPNVGR 442
>SPCC1020.03 [P] KOG1485 Mitochondrial Fe2+ transporter MMT1 and related
transporters (cation diffusion facilitator superfamily)
Length = 397
Score = 177 bits (448), Expect = 4e-44
Identities = 102/295 (34%), Positives = 156/295 (52%), Gaps = 16/295 (5%)
Query: 118 HAHSHGLGNPMLVLSREQFKN--NPGVRITWIGLLTNVGLAVGKFTGGVVFHSQALIADS 175
H H HG ML L + K +P +++ W+GL +N+GLA K GGV S L+AD+
Sbjct: 48 HTHDHGSDKEMLELVKALKKEGKSPELKLAWLGLYSNIGLAAAKGIGGVALQSSILVADA 107
Query: 176 VHAISDLVSDFLTLFSVGLATRKPTNDYPYGYGKVETIGSLAVSSILTTAGLSIGWSSLC 235
H + D +SD +TL ++ + ++KPT YP G+GK ETIG+ VS +L + I SSL
Sbjct: 108 AHQLGDTLSDLVTLATLKICSKKPTQKYPAGFGKWETIGTFTVSGLLVAVSVGIAHSSLS 167
Query: 236 AIAGPIVPHAILEFFAXXXXXXXXXXXXXXTDINAAWIAGGSIILKEWIYKATKKVALET 295
+ + P+A E A + GS++LKEW+++ T+ VA +T
Sbjct: 168 RLYTILFPYAGSE-HTHIGHSHNPSQLLFEHPFMALGLIFGSVVLKEWLFRKTRTVAQKT 226
Query: 296 KSNVLMANAWHHRVDSLTSLVALVTISSGYLFNIQSLDAVGGLIVSGLVVKAGTQGMVAS 355
SN+L+ANAWHHR D+LT +V+L+ +S Y N LD G +VS +V AG +
Sbjct: 227 DSNILLANAWHHRADALTGMVSLLALSGTYFLNAPWLDPFFGCLVSIVVFSAGFNSSKKA 286
Query: 356 IKELIDKSLPPTDSRYVEVETMTKEVLSKLISNNNSKKPYRIVDLTVMTSGPNMH 410
+L+D++ P + R + + K K PY+IV T++ + MH
Sbjct: 287 FLQLLDRA-PSEELRIAVTDALLK----------GEKLPYKIV--TILGNAHAMH 328
>At2g47830 [P] KOG1485 Mitochondrial Fe2+ transporter MMT1 and related
transporters (cation diffusion facilitator superfamily)
Length = 483
Score = 133 bits (334), Expect = 7e-31
Identities = 81/227 (35%), Positives = 124/227 (53%), Gaps = 11/227 (4%)
Query: 141 GVRITWIGLLTNVGLAVGKFTGGVVFHSQALIADSVHAISDLVSDFLTLFSVGLATRKPT 200
G +I +GL ++GL+V K G + S A+IAD+ H++SD+V + L S A
Sbjct: 73 GEKIFRLGLTADIGLSVAKALTGYLCGSTAIIADAAHSVSDVVLSGVALVSYRAANVPKD 132
Query: 201 NDYPYGYGKVETIGSLAVSSILTTAGLSIGWSS--LCAIAGPIVPHAILEFFAXXXXXXX 258
++PYG+GK ET+G+L +S++L G I W + L +IA P I
Sbjct: 133 KEHPYGHGKFETLGALGISAMLLATGSGIAWHALDLLSIALSAAPEVI--------HSGH 184
Query: 259 XXXXXXXTDINAAWIAGGSIILKEWIYKATKKVALETKSNVLMANAWHHRVDSLTSLVAL 318
I A + SI +KE +Y TK+ + S ++MANAWHHR D+++SLVAL
Sbjct: 185 HHGIDMNHPILALTVTIASISIKEGLYWITKRAGEKQGSGLMMANAWHHRSDAISSLVAL 244
Query: 319 VTISSGYLFNIQSLDAVGGLIVSGLVVKAGTQGMVASIKELIDKSLP 365
V + G + + LD + GL+VS ++V AG + SI EL+D ++P
Sbjct: 245 VGV-GGSILGVNFLDPLAGLVVSTMIVNAGLKTGHQSILELVDAAIP 290
>CE16617 [P] KOG1482 Zn2+ transporter
Length = 410
Score = 56.2 bits (134), Expect = 1e-07
Identities = 41/180 (22%), Positives = 70/180 (38%), Gaps = 23/180 (12%)
Query: 43 PENITEPPKKVDSSANDLSKDGTNAQEKLEDNPHFAKLAEDFSSHDHIHLQESETEENDS 102
P NI E + V SS + +S D ++ + H H H H ++ +D
Sbjct: 26 PANIEEDTQSVSSSDSGVSADS------IDHHHHGHGHGHSHGGHGHSHTHNNDDSSSDC 79
Query: 103 FKLGSMLTRTPQHANHAHSHGLGNPMLVLSREQFKNNPGVRITWIGLLTNVGLAVGKFTG 162
G H HSH + K ++ W + +F G
Sbjct: 80 SGAGG--------GAHKHSH---------DEKYQKGRRAEKVLWAVAALSAVFIAAEFVG 122
Query: 163 GVVFHSQALIADSVHAISDLVSDFLTLFSVGLATRKPTNDYPYGYGKVETIGSLAVSSIL 222
G S A++ D+ H +SDL+S +++F++ A + +GY + E +G+L IL
Sbjct: 123 GFWAQSLAIMTDAGHMLSDLLSFIISIFAIRCARLPASKRLSFGYERAEVLGALTSVIIL 182
>YOR316c [P] KOG1483 Zn2+ transporter ZNT1 and related Cd2+/Zn2+
transporters (cation diffusion facilitator superfamily)
Length = 439
Score = 53.5 bits (127), Expect = 7e-07
Identities = 36/102 (35%), Positives = 57/102 (55%), Gaps = 2/102 (1%)
Query: 142 VRITWIGLLTNVGLAVGKFTGGVVFHSQALIADSVHAISDLVSDFLTLFSVGLA-TRKPT 200
V+I + LL V + + T G + HS ALIADS H ++D++S + L++V +A R P
Sbjct: 8 VKIISLLLLDTVFFGI-EITTGYLSHSLALIADSFHMLNDIISLVVALWAVNVAKNRNPD 66
Query: 201 NDYPYGYGKVETIGSLAVSSILTTAGLSIGWSSLCAIAGPIV 242
+ Y YG+ + E +G+L + L +SI +L I P V
Sbjct: 67 STYTYGWKRAEILGALINAVFLIALCVSILIEALQRIIAPPV 108
>CE03664 [P] KOG1482 Zn2+ transporter
Length = 382
Score = 51.2 bits (121), Expect = 4e-06
Identities = 23/78 (29%), Positives = 44/78 (55%)
Query: 138 NNPGVRITWIGLLTNVGLAVGKFTGGVVFHSQALIADSVHAISDLVSDFLTLFSVGLATR 197
N RI W+ ++ + V + GGV+ S A++ D+ H ++D S ++LFS+ +A R
Sbjct: 69 NRRATRILWLTVVLCLFFMVCEVIGGVLAGSLAIVTDAAHLLTDFASVLISLFSLYIARR 128
Query: 198 KPTNDYPYGYGKVETIGS 215
P+ +G+ + E +G+
Sbjct: 129 PPSQKMSFGFHRAEVLGA 146
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,848,148
Number of Sequences: 60738
Number of extensions: 1030823
Number of successful extensions: 3285
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3272
Number of HSP's gapped (non-prelim): 8
length of query: 513
length of database: 30,389,216
effective HSP length: 111
effective length of query: 402
effective length of database: 23,647,298
effective search space: 9506213796
effective search space used: 9506213796
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)