ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII4063  suspect: LH   K     KOG3122  Transcription                                                  DNA-directed RNA polymerase III subunit

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII4063 1434661 1433450 -404 
         (404 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDL150w [K] KOG3122 DNA-directed RNA polymerase III subunit           267   2e-71
SPCC18.07 [K] KOG3122 DNA-directed RNA polymerase III subunit          54   3e-07

>YDL150w [K] KOG3122 DNA-directed RNA polymerase III subunit
          Length = 422

 Score =  267 bits (683), Expect = 2e-71
 Identities = 167/426 (39%), Positives = 232/426 (54%), Gaps = 33/426 (7%)

Query: 2   SGRLPGL----NQGKPSTGTSLKFKPKVVARKSKEERDANVLKV---EEPNKQVEKKKYT 54
           +GRLP L    + G  S   SLKFKPK VARKSKEER+A   KV   EE  +  +KK + 
Sbjct: 7   NGRLPSLKDSSSNGGGSAKPSLKFKPKAVARKSKEEREAAASKVKLEEESKRGNDKKHFN 66

Query: 55  QKPQ------GNQKRQPKYLNNTRIITSGPLAAXXXXXXXXXXXXXXIKTEGSQSSLVQR 108
            K +      G Q+R  KYLNNT +I+SGPLAA              IK+EGS SSLVQ+
Sbjct: 67  NKNKRVTGAGGQQRRMAKYLNNTHVISSGPLAAGNFVSEKGDLRRGFIKSEGSGSSLVQK 126

Query: 109 GLKXXXXXXXXXXXXX--------XXKAGKRINMGKEYTEADFKKETDDLFDEDEDSNTD 160
           GL+                       K+ K+ NMGKE+   +  ++ DD    + + ++D
Sbjct: 127 GLETIDNGAESSENEAEDDDNEGVASKSKKKFNMGKEFEARNLIEDEDD---GESEKSSD 183

Query: 161 AAMSEETRASKQLANLFPVRAVRINHEKVDSLQKVVQESMSTLQTREPTPGPVKLEPEQS 220
             M +E   SK++  LFPVR VR+ HE V+++++ +QE++S   TREPTP  VK EP  +
Sbjct: 184 VDMDDEEWRSKRIEQLFPVRPVRVRHEDVETVKREIQEALSEKPTREPTPS-VKTEPVGT 242

Query: 221 DGTLNDIVNXXXXXXXXXXXXXXXXXDFQSVDSAEALRETNLLFEDHKHMYKKLNKINDV 280
              L   +                  +FQSVD  EA  E  LL  DH+H+ +KL K+N+ 
Sbjct: 243 G--LQSYLEERERQVNEKLADLGLEKEFQSVDGKEAAAELELLNADHQHILRKLKKMNNK 300

Query: 281 PNKFMFFQFPSNLPEFQPQPPVKSTEEDVKPNGEAKVDEGSEQSNSKNSKETQIDSPEPL 340
           P +FM FQ P+ LP F+ +P VK  +ED++       D   ++ N K        S   L
Sbjct: 301 PERFMVFQLPTRLPAFE-RPAVKEEKEDMETQAS---DPSKKKKNIKKKDTKDALSTREL 356

Query: 341 IGNVGSIRMHRSGKMSIKIGDVVMDISRGAESTFLQDVV--TXXXXXXXXXXXXQIDGRA 398
            G VGSIR+H+SGK+S+KIG+VVMDI +GAE+TFLQDV+  +            ++DG+ 
Sbjct: 357 AGKVGSIRVHKSGKLSVKIGNVVMDIGKGAETTFLQDVIALSIADDASSAELLGRVDGKI 416

Query: 399 IVTPHI 404
           +VTP I
Sbjct: 417 VVTPQI 422


>SPCC18.07 [K] KOG3122 DNA-directed RNA polymerase III subunit
          Length = 330

 Score = 54.3 bits (129), Expect = 3e-07
 Identities = 35/133 (26%), Positives = 56/133 (41%), Gaps = 12/133 (9%)

Query: 282 NKFMFFQFPSNLPEFQ---------PQPPVKSTEEDVKPNGEAKVD---EGSEQSNSKNS 329
           ++ +  QFP  LP F          P   VK  E   K + E K D    G E  N  + 
Sbjct: 196 DQMVLMQFPDKLPRFMGDADVDPVWPSSEVKEEEAGEKTDVEEKKDPDKHGRENGNIDDL 255

Query: 330 KETQIDSPEPLIGNVGSIRMHRSGKMSIKIGDVVMDISRGAESTFLQDVVTXXXXXXXXX 389
                 +  P  G +G +R+H+SGK ++++G V   +  G++  FLQ++           
Sbjct: 256 TSLPYPAFHPPAGQIGVLRVHQSGKTTLEMGGVNFVVQSGSDCLFLQEIAVVDYDSKRIW 315

Query: 390 XXXQIDGRAIVTP 402
                + R IV+P
Sbjct: 316 NLGSFERRMIVSP 328


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.308    0.128    0.345 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,848,421
Number of Sequences: 60738
Number of extensions: 840632
Number of successful extensions: 4019
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4012
Number of HSP's gapped (non-prelim): 2
length of query: 404
length of database: 30,389,216
effective HSP length: 109
effective length of query: 295
effective length of database: 23,768,774
effective search space: 7011788330
effective search space used: 7011788330
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)