ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4123 good F KOG3984 Nucleotide transport and metabolism Purine nucleoside phosphorylase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII4123 1456403 1457320 306
(306 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR209c [F] KOG3984 Purine nucleoside phosphorylase 407 e-113
SPAC1805.16c [F] KOG3984 Purine nucleoside phosphorylase 269 3e-72
Hs4557801 [F] KOG3984 Purine nucleoside phosphorylase 253 2e-67
7292245 [F] KOG3984 Purine nucleoside phosphorylase 223 2e-58
CE17994 [F] KOG3984 Purine nucleoside phosphorylase 197 1e-50
7291629 [F] KOG3984 Purine nucleoside phosphorylase 134 2e-31
Hs18550128 [F] KOG3984 Purine nucleoside phosphorylase 69 8e-12
Hs4505273 [F] KOG3985 Methylthioadenosine phosphorylase MTAP 60 3e-09
7302794 [F] KOG3985 Methylthioadenosine phosphorylase MTAP 60 4e-09
>YLR209c [F] KOG3984 Purine nucleoside phosphorylase
Length = 311
Score = 407 bits (1046), Expect = e-113
Identities = 203/307 (66%), Positives = 241/307 (78%), Gaps = 4/307 (1%)
Query: 4 LDINEQRALIKSAHRYISEKLEDHFSSEFL---PKALVICGSGLSGISTKIA-DEPKPLI 59
L++++QR I A YIS LE HF + P+ L+ICGSGL GISTK++ D P P+
Sbjct: 5 LNVSQQREAITKAAAYISAILEPHFKNTTNFEPPRTLIICGSGLGGISTKLSRDNPPPVT 64
Query: 60 LSYSTIPGFKVSTVPGHSGELIFGYMNGAPVVLMNGRLHSYEGHSLAETVHPIRALHLLG 119
+ Y IPGFK STVPGHSG L+FG MNG+PVVLMNGRLH YEG++L ET PIR L+ +G
Sbjct: 65 VPYQDIPGFKKSTVPGHSGTLMFGSMNGSPVVLMNGRLHGYEGNTLFETTFPIRVLNHMG 124
Query: 120 SINVLIVTNAAGGINASFKAGDLMCVYDHINFPGLCGFHPLRGANFDEFGPRFLATSDAY 179
+ LIVTNAAGGINA ++A DLMC+YDH+N PGL G HPLRG N DE GPRFLA SDAY
Sbjct: 125 HVRNLIVTNAAGGINAKYQACDLMCIYDHLNIPGLAGQHPLRGPNLDEDGPRFLALSDAY 184
Query: 180 DLELRKLLFSKKKELNIERKIHEGTYSYVHGPTFESRAESRFLRLAGTDAVGMSTVPEVV 239
DLELRKLLF K KEL I+R +HEGTY++V GPTFE+RAES+ +R+ G DAVGMSTVPEV+
Sbjct: 185 DLELRKLLFKKWKELKIQRPLHEGTYTFVSGPTFETRAESKMIRMLGGDAVGMSTVPEVI 244
Query: 240 TARHCGWRVLALSLITNECVVDPPASAHDENPVPIQEGKATHEEVLENSAKASKDVQELI 299
ARHCGWRVLALSLITN CVVD PASA DE+PVP+++GKATH EVLEN AS DVQ LI
Sbjct: 245 VARHCGWRVLALSLITNTCVVDSPASALDESPVPLEKGKATHAEVLENGKIASNDVQNLI 304
Query: 300 FSVVAEI 306
+V+ E+
Sbjct: 305 AAVMGEL 311
>SPAC1805.16c [F] KOG3984 Purine nucleoside phosphorylase
Length = 315
Score = 269 bits (688), Expect = 3e-72
Identities = 147/297 (49%), Positives = 190/297 (63%), Gaps = 19/297 (6%)
Query: 16 AHRYISEKLEDHFSSEFLPKALVICGSGL----SGISTKIADEPKPLILSYSTIPGFKVS 71
A YI E++ + S PK +ICGSGL SG+S + + P Y IP F VS
Sbjct: 26 AREYIIEQVPEELSK---PKVAIICGSGLGTLASGLSAPVYEVP------YEDIPHFHVS 76
Query: 72 TVPGHSGELIFGYMNG--APVVLMNGRLHSYEGHSLAETVHPIRALHLLGSINVLIVTNA 129
VPGH+ +L F ++ P +++ GR HSYEG+ + T P+R + ++G + V++VTNA
Sbjct: 77 HVPGHASKLYFAFLGEKRVPTMILAGRYHSYEGYPIEATTFPVRLMKVMG-VEVMVVTNA 135
Query: 130 AGGINASFKAGDLMCVYDHINFPGLCGFHPLRGANFDEFGPRFLATSDAYDLELRKLLFS 189
AGG+N FK GDLM + DHINFPGL G +PLRG N EFG RF SDAYDLELRKL++
Sbjct: 136 AGGLNQGFKVGDLMILKDHINFPGLAGMNPLRGPNAHEFGVRFPPLSDAYDLELRKLVYD 195
Query: 190 KKKELNIERKIHEGTYSYVHGPTFESRAESRFLRLAGTDAVGMSTVPEVVTARHCGWRVL 249
K + R IHEG Y++V GP FE+RAESR L L G D VGMSTVPEVV ARHCG RVL
Sbjct: 196 AAKAHKVSRTIHEGCYAFVSGPCFETRAESRMLALMGADCVGMSTVPEVVVARHCGIRVL 255
Query: 250 ALSLITNECVVDPPASAHDENPVP---IQEGKATHEEVLENSAKASKDVQELIFSVV 303
A+SL+TN VV+ SA D V + +G A H EVLE A+ DV+ ++ ++V
Sbjct: 256 AISLVTNNVVVEESPSAKDLVEVDSNVMSKGAANHLEVLEVGIAAAADVRTMVETIV 312
>Hs4557801 [F] KOG3984 Purine nucleoside phosphorylase
Length = 289
Score = 253 bits (646), Expect = 2e-67
Identities = 129/273 (47%), Positives = 175/273 (63%), Gaps = 15/273 (5%)
Query: 34 PKALVICGSGLSGISTKIADEPKPLILSYSTIPGFKVSTVPGHSGELIFGYMNGAPVVLM 93
P+ +ICGSGL G++ K+ I YS IP F STVPGH+G L+FG++NG V+M
Sbjct: 25 PQVAIICGSGLGGLTDKLTQAQ---IFDYSEIPNFPRSTVPGHAGRLVFGFLNGRACVMM 81
Query: 94 NGRLHSYEGHSLAETVHPIRALHLLGSINVLIVTNAAGGINASFKAGDLMCVYDHINFPG 153
GR H YEG+ L + P+R HLLG ++ L+VTNAAGG+N F+ GD+M + DHIN PG
Sbjct: 82 QGRFHMYEGYPLWKVTFPVRVFHLLG-VDTLVVTNAAGGLNPKFEVGDIMLIRDHINLPG 140
Query: 154 LCGFHPLRGANFDEFGPRFLATSDAYDLELRKLLFSKKKELNIERKIHEGTYSYVHGPTF 213
G +PLRG N + FG RF A SDAYD +R+ S K++ +R++ EGTY V GP+F
Sbjct: 141 FSGQNPLRGPNDERFGDRFPAMSDAYDRTMRQRALSTWKQMGEQRELQEGTYVMVAGPSF 200
Query: 214 ESRAESRFLRLAGTDAVGMSTVPEVVTARHCGWRVLALSLITNECVVDPPASAHDENPVP 273
E+ AE R L+ G DAVGMSTVPEV+ ARHCG RV SLITN+ ++D +
Sbjct: 201 ETVAECRVLQKLGADAVGMSTVPEVIVARHCGLRVFGFSLITNKVIMDYESLE------- 253
Query: 274 IQEGKATHEEVLENSAKASKDVQELIFSVVAEI 306
KA HEEVL +A++ +++ + ++A I
Sbjct: 254 ----KANHEEVLAAGKQAAQKLEQFVSILMASI 282
>7292245 [F] KOG3984 Purine nucleoside phosphorylase
Length = 353
Score = 223 bits (569), Expect = 2e-58
Identities = 127/305 (41%), Positives = 172/305 (55%), Gaps = 16/305 (5%)
Query: 5 DINEQRALIKSAHRY-ISEKLEDHFS--SEFLPKALVICGSGLSGISTKIADEPKPLILS 61
++ R L + + Y + E++ D + S PK +ICGSGL ++ I D P I
Sbjct: 57 ELRALRVLNEDTYPYEVIEEIADFITKGSGMRPKIGIICGSGLGSLADMIQD---PKIFE 113
Query: 62 YSTIPGFKVSTVPGHSGELIFGYMNGAPVVLMNGRLHSYEGHSLAETVHPIRALHLLGSI 121
Y IP F VSTV GH+G L+ G + GA V+ M GR H YEG+ LA+ P+R + L G +
Sbjct: 114 YEKIPNFPVSTVEGHAGRLVVGTLEGATVMAMQGRFHFYEGYPLAKCSMPVRVMKLCG-V 172
Query: 122 NVLIVTNAAGGINASFKAGDLMCVYDHINFPGLCGFHPLRGANFDEFGPRFLATSDAYDL 181
L TNAAGGIN F GD+M ++DH+N G G PL+G N FGPRF A ++Y+
Sbjct: 173 EYLFATNAAGGINPRFAVGDIMLMHDHVNMLGFAGNSPLQGPNDPRFGPRFPALVNSYNK 232
Query: 182 ELRKLLFSKKKELNIERKIHEGTYSYVHGPTFESRAESRFLRLAGTDAVGMSTVPEVVTA 241
+L K + IE IH G YS + GP +E+ AE + LR+ G DAVGMSTV EV+TA
Sbjct: 233 DLINKAIEIAKAMGIESNIHVGVYSCLGGPNYETIAELKALRMMGVDAVGMSTVHEVITA 292
Query: 242 RHCGWRVLALSLITNECVVDPPASAHDENPVPIQEGKATHEEVLENSAKASKDVQELIFS 301
RHC +V A SLITN+C + DE A H+EV+ + K EL+
Sbjct: 293 RHCDMKVFAFSLITNKCATEYSDKKDDE---------ANHDEVMAVAKNRQKACCELVSR 343
Query: 302 VVAEI 306
++ EI
Sbjct: 344 LIREI 348
>CE17994 [F] KOG3984 Purine nucleoside phosphorylase
Length = 301
Score = 197 bits (502), Expect = 1e-50
Identities = 114/270 (42%), Positives = 152/270 (56%), Gaps = 18/270 (6%)
Query: 38 VICGSGLSGISTKIADEPKPLILSYSTIPGFKVSTVPGHSGELIFGYMNGAPVVLMNGRL 97
+ICGSGL I + D IL YS IPGF + V GH G +IFG + G VV + GR
Sbjct: 47 IICGSGLGPIGDTVQDAT---ILPYSKIPGFPTTHVVGHKGNMIFGKLGGKKVVCLQGRF 103
Query: 98 HSYEGH-SLAETVHPIRALHLLGSINVLIVTNAAGGINASFKAGDLMCVYDHINFPGLCG 156
H YE + LA P+R +H LG I ++IV+NAAGGINA + GDLM + DHI P L G
Sbjct: 104 HPYEHNMDLALCTLPVRVMHQLG-IKIMIVSNAAGGINAVLRHGDLMLIKDHIFLPALAG 162
Query: 157 FHPLRGANFDEFGPRFLATSDAYDLELRKLLFSKKKELNIERKIHEGTYSYVHGPTFESR 216
F PL G N FG RF++ DAYD +LR+L + ++ ++EG Y GP +ES
Sbjct: 163 FSPLVGCNDPRFGARFVSVHDAYDKQLRQLAIDVGRRSDM--TLYEGVYVMSGGPQYESP 220
Query: 217 AESRFLRLAGTDAVGMSTVPEVVTARHCGWRVLALSLITNECVVDPPASAHDENPVPIQE 276
AE + G DA+GMST EV AR CG +VL SLITN +D AS
Sbjct: 221 AEVSLFKTVGADALGMSTCHEVTVARQCGIKVLGFSLITNIANLDADASV---------- 270
Query: 277 GKATHEEVLENSAKASKDVQELIFSVVAEI 306
+ +HEEV++ + +A + + ++ EI
Sbjct: 271 -EVSHEEVMDIAQQAGERASRFVSDIITEI 299
>7291629 [F] KOG3984 Purine nucleoside phosphorylase
Length = 339
Score = 134 bits (336), Expect = 2e-31
Identities = 78/226 (34%), Positives = 121/226 (53%), Gaps = 6/226 (2%)
Query: 30 SEFLPKALVICGSGLSGISTKIADEPKPLILSYSTIPGFKVSTVPGHSGELIFGYMNGAP 89
S PK +ICGS LS + + + +P+++ Y IP F P S + G + GAP
Sbjct: 61 SHIRPKYGLICGSFLSDMVSLVE---QPVVIPYEDIPNFPDGIEPDCS--FVLGTVMGAP 115
Query: 90 VVLMNGRLHSYEGHSLAETVHPIRALHLLGSINVLIVTNAAGGINASFKAGDLMCVYDHI 149
++ + HS +G++LA P+R + L G + +++T+ A ++ F GD+M V DHI
Sbjct: 116 IIALVHSFHSCDGYNLATCALPVRVMQLCG-VRTIMLTSEAAAVDHGFALGDIMLVQDHI 174
Query: 150 NFPGLCGFHPLRGANFDEFGPRFLATSDAYDLELRKLLFSKKKELNIERKIHEGTYSYVH 209
N G+ PL G + FG R + +AYD +L + K + I++ +H G + +
Sbjct: 175 NVVGMMHQTPLEGPSDPRFGSRRFSMVNAYDKDLLEKALEIGKRMGIQKFLHSGVLACMG 234
Query: 210 GPTFESRAESRFLRLAGTDAVGMSTVPEVVTARHCGWRVLALSLIT 255
GP + AE R LR AVGMS VPEV+ A H G +VLA +I+
Sbjct: 235 GPILGTVAEERMLRTMEVSAVGMSLVPEVIAAHHGGLKVLAFVVIS 280
>Hs18550128 [F] KOG3984 Purine nucleoside phosphorylase
Length = 97
Score = 68.9 bits (167), Expect = 8e-12
Identities = 35/93 (37%), Positives = 53/93 (56%), Gaps = 1/93 (1%)
Query: 93 MNGRLHSYEGHSLAETVHPIRALHLLGSINVLIVTNAAGGINASFKAGDLMCVYDHINFP 152
M GR + Y+G+ L + LLG N+L+ T+AAGG+N + G +M + DHI
Sbjct: 1 MQGRFYLYDGYLLWNMIFLHEVFQLLGG-NILVATDAAGGLNPKSEVGRIMLLCDHIKLL 59
Query: 153 GLCGFHPLRGANFDEFGPRFLATSDAYDLELRK 185
G C + +G N + FG F ATSDAY+ +++
Sbjct: 60 GFCDQNSPKGPNDERFGVHFPATSDAYNWTMKQ 92
>Hs4505273 [F] KOG3985 Methylthioadenosine phosphorylase MTAP
Length = 283
Score = 60.5 bits (145), Expect = 3e-09
Identities = 51/182 (28%), Positives = 87/182 (47%), Gaps = 5/182 (2%)
Query: 77 SGELIFGYMNGAPVVLM--NGRLHSYEGHSLAETVHPIRALHLLGSINVLIVTNAAGGIN 134
S LI G + +L+ +GR H+ + + I AL G +V IVT A G +
Sbjct: 42 SDALILGKIKNVDCILLARHGRQHTIMPSKVNYQAN-IWALKEEGCTHV-IVTTACGSLR 99
Query: 135 ASFKAGDLMCVYDHINFPGLCGFHPLRGANFDEFGPRFLATSDAYDLELRKLLFSKKKEL 194
+ GD++ + I+ + G++ G + ++ + + R++L K+L
Sbjct: 100 EEIQPGDIVIIDQFIDRTTMRPQSFYDGSHSCARGVCHIPMAEPFCPKTREVLIETAKKL 159
Query: 195 NIERKIHEGTYSYVHGPTFESRAESRFLRLAGTDAVGMSTVPEVVTARHCGWRVLALSLI 254
+ R +GT + GP F SRAES R G D + M+TVPEVV A+ G ++++
Sbjct: 160 GL-RCHSKGTMVTIEGPRFSSRAESFMFRTWGADVINMTTVPEVVLAKEAGICYASIAMA 218
Query: 255 TN 256
T+
Sbjct: 219 TD 220
>7302794 [F] KOG3985 Methylthioadenosine phosphorylase MTAP
Length = 289
Score = 60.1 bits (144), Expect = 4e-09
Identities = 57/184 (30%), Positives = 85/184 (45%), Gaps = 9/184 (4%)
Query: 77 SGELIFGYMNGAPVVLM--NGRLHSYEGHSLAETVHPIRALHLLGSINVLIVTNAAGGIN 134
S LI G +NG VL+ +GR H ++ + I AL +G + LIV+ A G +
Sbjct: 48 SDALIEGEINGVQCVLLARHGRKHDIMPSNVNYRAN-IWALRDVGCTH-LIVSTACGSLR 105
Query: 135 ASFKAGDLMCVYDHIN--FPGLCGFHPLRGANFDEFGPRFLATSDAYDLELRKLLFSKKK 192
K G+L+ +D I+ L F+ G G L A+ R +L K
Sbjct: 106 EEIKPGNLVVPHDFIDRTTKRLQTFYD--GKAQSPRGVCHLPMFPAFSERTRNILLQAAK 163
Query: 193 ELNIERKIHEGTYSYVHGPTFESRAESRFLRLAGTDAVGMSTVPEVVTARHCGWRVLALS 252
EL I + T + GP F SR+ES R G D + M+T PEVV A+ G +++
Sbjct: 164 ELEIPAH-DKATIVTIEGPRFSSRSESHMFRQWGGDLINMTTCPEVVLAKEAGLLYGSVA 222
Query: 253 LITN 256
+ T+
Sbjct: 223 IATD 226
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.136 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,702,466
Number of Sequences: 60738
Number of extensions: 812954
Number of successful extensions: 2067
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2040
Number of HSP's gapped (non-prelim): 9
length of query: 306
length of database: 30,389,216
effective HSP length: 106
effective length of query: 200
effective length of database: 23,950,988
effective search space: 4790197600
effective search space used: 4790197600
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)