ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII4196  suspect: LH   R     KOG0274  General function prediction only                               Cdc4 and related F-box and WD-40 proteins

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII4196 1481469 1479544 -642 
         (642 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YML088w [R] KOG0274 Cdc4 and related F-box and WD-40 proteins         419   e-117

>YML088w [R] KOG0274 Cdc4 and related F-box and WD-40 proteins
          Length = 668

 Score =  419 bits (1077), Expect = e-117
 Identities = 238/612 (38%), Positives = 352/612 (56%), Gaps = 41/612 (6%)

Query: 1   MSRPSL--SSLPVEVLVNIFSHLDDSDFRSLEQTCKLFDRIVHDEELWKNLFLQRHNTLW 58
           M RP L    LP E+L+NIFSHLD+ D  +L++    F  ++HDEELWKNLF  R +T  
Sbjct: 1   MERPGLVLQDLPPEILINIFSHLDEKDLFTLQELSTHFRNLIHDEELWKNLFKSRVHTTH 60

Query: 59  FPSFSRSNLYSMEYIKRNMALNEWRHNRATKTKYTITHDVSQQPQDISRVVFDFPRCACY 118
           FP+FS+S+ +S+EYI+R   L+ W+HN+A +TKYTI    +     I R+VFD+PR A Y
Sbjct: 61  FPTFSQSSKFSVEYIERTRGLHHWQHNKAIRTKYTIIPTRNWDQPSIERIVFDYPRVAAY 120

Query: 119 NEGMVTFLQLQ---KKRKKDRVAYIQCTTPHGTSARHFNINAVVFGRYDGRVFGKLLTNK 175
           N+G +T LQLQ   +++K  ++ YI CTTP G S   FNINA VFGR+DGRVFGKLL+NK
Sbjct: 121 NDGTITILQLQNHKRQKKFKKLIYIPCTTPQGCSTMDFNINAAVFGRFDGRVFGKLLSNK 180

Query: 176 SYLSPVTEFDDRHYSAVTAIVTT-AYQDSPDDVCVSGDESGEVIWWRNTQKEKKMKISSH 234
           SYL+PV EF  RH + VTAI  + ++  S +D  VSG E+GE+IWW   +  K  K+S+ 
Sbjct: 181 SYLTPVMEFTGRHSAGVTAICNSESWDTSREDWSVSGSENGEIIWWCENKLVKMWKVSNR 240

Query: 235 PIADLHVHKNTTIAIDLETIYVIKSMEEVHSIDLIAKIGTDFSNCKVTKVDFGGRNIVVA 294
            I  L   K+ T+ +D E +Y+I  M+E+HSID+   +       +  K+DFG   +V+A
Sbjct: 241 VIWKLAFFKDWTLIMDDEKLYIIHQMQELHSIDIPKDLDEQPMRVRFFKMDFGSMTLVLA 300

Query: 295 TLFEIYVISIDIGKDFGRTRPMFFLSAIQEVSIDEATAKKERDLSLAGGDGCYIAVLTED 354
            L  +Y IS++   +FG  R +     I  V IDE T+++E++   AG DGCYI++LT  
Sbjct: 301 DLNNVYTISVNPNGNFGNLRKLEMPEQICAVEIDEKTSQREQNWQFAGDDGCYISLLTTQ 360

Query: 355 KNVSTINIR-MPGVDLECQSTVSLHESWVTCQINNLVVVCAFSGMIGVYDVMTGNEIRVI 413
             +  INIR +    L+ Q  +S  E     Q+ NL+VV A   ++ + + MTG  I+ +
Sbjct: 361 NTLYIINIRDLSSSGLKVQCKISFDEQVYVSQVTNLIVVVALPNVLQILNAMTGELIKTV 420

Query: 414 RNSEHYPEFLNISHGQILVGKESTLYYYQYVSIGD--HSKKKRGGNRARSNKWNENLQQQ 471
             +E +PEFL +S  +I++G  + L Y ++VS     H    +G N   SNKWNE L  +
Sbjct: 421 LKTEKFPEFLKVSQDKIIMGSGNVLNYLKFVSSDSKKHHHSTKGKNTV-SNKWNETLNTE 479

Query: 472 LKMYDDEEQKRITDRNYMDKLKKKFVGDID-----DEE--VQFQIALMEXXXXXXXXXX- 523
           L+ YD++E  R   ++ + +L   + GD++     DEE  +Q +IAL+E           
Sbjct: 480 LQYYDEDEDLRRKRQSEISRLIDAYGGDLELSGDTDEENDIQLRIALLESQEAQARNQAE 539

Query: 524 ---------------LNPEDHLLGSQESNST------TNQFIDDDEIDVDFLKALQDSET 562
                             E  L+   ++NS+      TN  ID+D  D +FL+A++ S  
Sbjct: 540 AGEPVGDDEDEQLRRALEESQLIYETQTNSSANHGNNTNDEIDED--DEEFLRAIRQSRV 597

Query: 563 QERRAEKRRERT 574
           ++ R    R  T
Sbjct: 598 EDERRRHLRNHT 609


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.317    0.133    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,923,623
Number of Sequences: 60738
Number of extensions: 1668741
Number of successful extensions: 5926
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 5921
Number of HSP's gapped (non-prelim): 1
length of query: 642
length of database: 30,389,216
effective HSP length: 113
effective length of query: 529
effective length of database: 23,525,822
effective search space: 12445159838
effective search space used: 12445159838
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)