ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4351 good E KOG2348 Amino acid transport and metabolism Urea transporter
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII4351 1534939 1532771 -723
(723 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHL016c [E] KOG2348 Urea transporter 850 0.0
SPBC23G7.13c [E] KOG2348 Urea transporter 597 e-170
At5g45380 [E] KOG2348 Urea transporter 421 e-117
SPAC869.03c [E] KOG2348 Urea transporter 404 e-112
>YHL016c [E] KOG2348 Urea transporter
Length = 735
Score = 850 bits (2196), Expect = 0.0
Identities = 423/720 (58%), Positives = 518/720 (71%), Gaps = 20/720 (2%)
Query: 1 MAVMEAPLPQXXXXXXXXXXXXXXXXXMILTTYVLRRYQKEIITAEEFATAGRSVKTGLI 60
M + PLPQ M+LTTY+L+RYQKEIITAEEF TAGRSVKTGL+
Sbjct: 1 MGEFKPPLPQGAGYAIVLGLGAVFAGMMVLTTYLLKRYQKEIITAEEFTTAGRSVKTGLV 60
Query: 61 XXXXXXXXXXXXXLLQSTTMVYKVGISXXXXXXXXXXXQVILFSALAIKCKQRAPNAHTY 120
LL S+T Y GI Q+I F+ LAIK KQ APNAHTY
Sbjct: 61 AAAVVSSWIWCSTLLTSSTKEYADGIFGGYAYAAGACFQIIAFAILAIKTKQMAPNAHTY 120
Query: 121 LEIIKARYGTIGHFCYMFYALATNVLVTAMLLTGGSAVVSDLTGMHTVAACFLLPVGVVL 180
LE+++ RYG IGH CY+FYA+ATN+LVT+MLLT GSAV SDLTGM+T+A+CFLLPVGVV+
Sbjct: 121 LELVRTRYGKIGHGCYLFYAIATNILVTSMLLTSGSAVFSDLTGMNTIASCFLLPVGVVV 180
Query: 181 YTIFGGIKATFLTDYVHTXXXXXXXXXFAFTVYATNSQLGSPKAVYDLVREAAKAHPIEG 240
YT+FGGIKATFLTDY+HT FAF VYAT+ LGSP VYDLVREAAK HP++G
Sbjct: 181 YTLFGGIKATFLTDYMHTCVIIIIVLVFAFKVYATSDVLGSPGKVYDLVREAAKRHPVDG 240
Query: 241 NAGGEYLTMRSRSGGIFFVINIVGNFGTVFLDAGYWNKAISSSPAAALPGYVLGGLSWIA 300
N GEY+TM S+S GI +IN++GNFGTVFLD GYWNKAIS+SPAA+L Y +GGL+W A
Sbjct: 241 NYQGEYMTMTSKSAGILLIINLIGNFGTVFLDNGYWNKAISASPAASLKAYAIGGLAWFA 300
Query: 301 IPNLISLAMGLACVALESSPNFPTYPERLTAEQVSAGLVLPTAAVTLLGKGGAVASLLLV 360
+P+LISL MGLAC+A+E+SPNFPTYP+ LT+ Q ++GLVLP AA+ ++GKGGAVASLL++
Sbjct: 301 VPSLISLTMGLACLAVETSPNFPTYPDPLTSFQANSGLVLPAAAIAIMGKGGAVASLLMI 360
Query: 361 FMAVTSAMSAELIAVSSIFTYDIYRSYLKPKATGKQLIFSSHISCIVFGLIMSGFATGLY 420
FMAVTSAMSAELIAVSS+FTYDIYR Y+ P+A+GK+LI++SH++CI FGL MSGF+ GLY
Sbjct: 361 FMAVTSAMSAELIAVSSVFTYDIYREYIDPRASGKKLIYTSHVACIFFGLAMSGFSVGLY 420
Query: 421 YAGISMGYLYELMGIIISSAVIPCALSLFWDAQNLVAVVASPIIGTSLAIMSWLVCTKSL 480
Y GISMGY+YE+MGIIISSAV+P L+L NLVA V SPI+GT LAIMSWLVCTKSL
Sbjct: 421 YGGISMGYIYEMMGIIISSAVLPVVLTLCSKDMNLVAAVVSPILGTGLAIMSWLVCTKSL 480
Query: 481 YGEITVSTTFEDDPMLTGNIVAXXXXXXXXXXXXYIFKPQNFDWEILKTITRADXXXXXX 540
Y E+TV TTF D PMLTGN+VA Y+FKPQNFDWE +K ITR D
Sbjct: 481 YKELTVDTTFMDYPMLTGNLVALLSPAIFIPILTYVFKPQNFDWEKMKDITRVDETAELV 540
Query: 541 XXXXXXXXXXXXXXXXXXQDA-----------------EKLQAVKTVITTVDEIPRE--- 580
++ EKL + + I +E
Sbjct: 541 QADPDIQLYDAEANDKEQEEETNSLVSDSEKNDVRVNNEKLIEPNLGVVISNAIFQEDDT 600
Query: 581 TIERMAEEESQYLSRASKIAGYLAIFFAISFMVLWPMPMYGTGYIFSEKFFTGWVCVLII 640
++ +EE + L+R KIA +L +FFA++F+V+WPMPMYG+ YIFS+KFFTGWV V+II
Sbjct: 601 QLQNELDEEQRELARGLKIAYFLCVFFALAFLVVWPMPMYGSKYIFSKKFFTGWVVVMII 660
Query: 641 WIFFTAFCVCCYPLWEGRHGIYTTVRGIYWDLSGQTYKLREWQDANPREMKAVQSQIIAK 700
W+FF+AF VC YPLWEGRHGIYTT+RG+YWDLSGQTYKLREWQ++NP+++ V SQI A+
Sbjct: 661 WLFFSAFAVCIYPLWEGRHGIYTTLRGLYWDLSGQTYKLREWQNSNPQDLHVVTSQISAR 720
>SPBC23G7.13c [E] KOG2348 Urea transporter
Length = 664
Score = 597 bits (1540), Expect = e-170
Identities = 301/649 (46%), Positives = 413/649 (63%), Gaps = 47/649 (7%)
Query: 28 MILTTYVLRRYQKEIITAEEFATAGRSVKTGLIXXXXXXXXXXXXXLLQSTTMVYKVGIS 87
MI ++ L+++ E T+E F TA SV+TGL+ LL S Y+ G+S
Sbjct: 26 MIFVSWSLKKFNNENQTSEHFNTASHSVRTGLVASAVVSSWTWASTLLTSAQKTYQYGVS 85
Query: 88 XXXXXXXXXXXQVILFSALAIKCKQRAPNAHTYLEIIKARYGTIGHFCYMFYALATNVLV 147
Q++LF+ LAI+ K++APNAHT+LE+++AR G I H ++ +A TN+LV
Sbjct: 86 GAFWYASGACVQILLFTVLAIELKRKAPNAHTFLEVVRARCGPIAHGVFLVFAYITNILV 145
Query: 148 TAMLLTGGSAVVSDLTGMHTVAACFLLPVGVVLYTIFGGIKATFLTDYVHTXXXXXXXXX 207
AMLL GGSA +S +TGM+TVA CFLLPVGV++YT+FGGIKATFLTDY+HT
Sbjct: 146 MAMLLCGGSATISSVTGMNTVAVCFLLPVGVIIYTMFGGIKATFLTDYIHTVIILVILIM 205
Query: 208 FAFTVYATNSQLGSPKAVYDLVREAAKAHPIEGNAGGEYLTMRSRSGGIFFVINIVGNFG 267
F+ Y+ + ++GSP +YD+++EA AHP+ GNA G YLTMRS+ G IFF+IN+ GNFG
Sbjct: 206 FSLATYSADKKIGSPGKLYDMLKEAGDAHPVAGNAQGSYLTMRSQEGAIFFIINLAGNFG 265
Query: 268 TVFLDAGYWNKAISSSPAAALPGYVLGGLSWIAIPNLISLAMGLACVALESSPNFPTYPE 327
TVF+D GYW KAI+++PA+ALPGY+LGGL+W AIP L + MGL + LE+ P FPTYP
Sbjct: 266 TVFVDNGYWQKAIAANPASALPGYILGGLAWFAIPWLAATTMGLVALGLENKPYFPTYPN 325
Query: 328 RLTAEQVSAGLVLPTAAVTLLGKGGAVASLLLVFMAVTSAMSAELIAVSSIFTYDIYRSY 387
R++ +VS GLVLP AA+ L+G+ GA A+LLLVFMAVTSA SAELIAVSSIFTYDIY+ Y
Sbjct: 326 RMSDLEVSEGLVLPYAAIALMGRAGANATLLLVFMAVTSAASAELIAVSSIFTYDIYKQY 385
Query: 388 LKPKATGKQLIFSSHISCIVFGLIMSGFATGLYYAGISMGYLYELMGIIISSAVIPCALS 447
++P+ATGK+L+++ H S IVFG MSGFATGLYY +SMGYLY LMG+++ AV+P
Sbjct: 386 VRPRATGKELLYTGHASLIVFGFAMSGFATGLYYGQVSMGYLYLLMGVLVCPAVVPATCV 445
Query: 448 LFWDAQNLVAVVASPIIGTSLAIMSWLVCTKSLYGE-ITVSTTFEDDPMLTGNIVAXXXX 506
+ + + +AV SP++G +I++WLV ++ G+ +T+ TT ++PML GN+V
Sbjct: 446 MLFSRVSTIAVTVSPVLGIISSIITWLVVARAEGGKTLTIETTGANNPMLAGNVVGLLSP 505
Query: 507 XXXXXXXXYIFKPQNFDWEILKTITRADXXXXXXXXXXXXXXXXXXXXXXXXQDAEKLQA 566
IF P+ +D+ L + +++Q
Sbjct: 506 ALYILILSIIF-PEKYDFNRL----------------------LATFAMHFSSEEDEIQQ 542
Query: 567 VKTVITTVDEIPRETIERMAEEESQYLSRASKIAGYLAIFFAISFMVLWPMPMYGTGYIF 626
K L+RAS I+ A+ +F++LWP PMYGTGYIF
Sbjct: 543 TKK-----------------------LNRASVISKVAALIITAAFIILWPWPMYGTGYIF 579
Query: 627 SEKFFTGWVCVLIIWIFFTAFCVCCYPLWEGRHGIYTTVRGIYWDLSGQ 675
S++FFTGWV V +IWIFFT F V +PLWEGR+ IY V + + G+
Sbjct: 580 SKRFFTGWVVVGLIWIFFTVFAVGIFPLWEGRNDIYQVVSNMAASIFGR 628
>At5g45380 [E] KOG2348 Urea transporter
Length = 694
Score = 421 bits (1081), Expect = e-117
Identities = 244/649 (37%), Positives = 351/649 (53%), Gaps = 74/649 (11%)
Query: 36 RRYQKEIITAEEFATAGRSVKTGLIXXXXXXXXXXXXXLLQSTTMVYKVGISXXXXXXXX 95
+RY T+E F TAGR+VKTGLI +LQS+ + ++ G+S
Sbjct: 61 KRYVGARHTSEWFNTAGRNVKTGLIASVIVSQWTWAATILQSSNVAWQYGVSGPFWYASG 120
Query: 96 XXXQVILFSALAIKCKQRAPNAHTYLEIIKARYGTIGHFCYMFYALATNVLVTAMLLTGG 155
QV+LF +AI+ K++APNAHT EI+KAR+GT H ++ + LATNV+VTAMLL GG
Sbjct: 121 ATIQVLLFGVMAIEIKRKAPNAHTVCEIVKARWGTATHIVFLVFCLATNVVVTAMLLLGG 180
Query: 156 SAVVSDLTGMHTVAACFLLPVGVVLYTIFGGIKATFLTDYVHTXXXXXXXXXFAFTVYAT 215
SAVV+ LTG++ AA FL+P+GVV+YT+ GG+KATFL YVH+ F F VY +
Sbjct: 181 SAVVNALTGVNLYAASFLIPLGVVVYTLAGGLKATFLASYVHSVIVHVALVVFVFLVYTS 240
Query: 216 NSQLGSPKAVYDLVRE-AAKAH--------------PIEGNAGGEYLTMRSRSGGIFFVI 260
+ +LGSP VYD +++ AK+ P++GN G YLTM S G +F +I
Sbjct: 241 SKELGSPSVVYDRLKDMVAKSRSCTEPLSHHGQACGPVDGNFRGSYLTMLSSGGAVFGLI 300
Query: 261 NIVGNFGTVFLDAGYWNKAISSSPAAALPGYVLGGLSWIAIPNLISLAMGLACVALESSP 320
NIVGNFGTVF+D GYW AI++ P++ GY+LGGL W A+P ++ ++GL +AL+
Sbjct: 301 NIVGNFGTVFVDNGYWVSAIAARPSSTHKGYLLGGLVWFAVPFSLATSLGLGALALDLP- 359
Query: 321 NFPTYPERLTAEQVSAGLVLPTAAVTLLGKGGAVASLLLVFMAVTSAMSAELIAVSSIFT 380
++ ++ GLV P A+ L+GK G++ L ++FMAVTSA S+ELIAVSS+FT
Sbjct: 360 --------ISKDEADRGLVPPATAIALMGKSGSLLLLTMLFMAVTSAGSSELIAVSSLFT 411
Query: 381 YDIYRSYLKPKATGKQLIFSSHISCIVFGLIMSGFATGLYYAGISMGYLYELMGIIISSA 440
YDIYR+Y+ P+ATG+Q++ S + + FG M A L AG+S+G++Y MG++I SA
Sbjct: 412 YDIYRTYINPRATGRQILKISRCAVLGFGCFMGILAVVLNKAGVSLGWMYLAMGVLIGSA 471
Query: 441 VIPCALSLFWDAQNLVAVVASPIIGTSLAIMSWLVCTKSLYGEITVSTTFEDDPMLTGNI 500
VIP A L W N + G I++WL K+ YG + + +T ++ PML GN+
Sbjct: 472 VIPIAFMLLWSKANAFGAILGATSGCVFGIITWLTTAKTQYGRVDLDSTGKNGPMLAGNL 531
Query: 501 VAXXXXXXXXXXXXYIFKPQNFDWEILKTITRADXXXXXXXXXXXXXXXXXXXXXXXXQD 560
VA +PQN+DW + I
Sbjct: 532 VAILT-----------VRPQNYDWSTTREI------------------------------ 550
Query: 561 AEKLQAVKTVITTVDEIPRETIERMAEEESQYLSRASKIAGYLAIFFAISFMVLWPMPMY 620
V + DE E + EE+ L RA + F I +V+WP+ +
Sbjct: 551 -----KVVEAYASGDEDVDVPAEELREEK---LRRAKAWIVKWGLVFTILIVVIWPV-LS 601
Query: 621 GTGYIFSEKFFTGWVCVLIIWIFFTAFCVCCYPLWEGRHGIYTTVRGIY 669
+FS +F W V I W + + PL E I + G++
Sbjct: 602 LPARVFSRGYFWFWAIVAIAWGTIGSIVIIGLPLVESWDTIKSVCMGMF 650
>SPAC869.03c [E] KOG2348 Urea transporter
Length = 661
Score = 404 bits (1038), Expect = e-112
Identities = 228/636 (35%), Positives = 337/636 (52%), Gaps = 45/636 (7%)
Query: 28 MILTTYVLRRYQKEIITAEEFATAGRSVKTGLIXXXXXXXXXXXXXLLQSTTMVYKVGIS 87
MIL TYVL+RY E+ +E F TA RSVKTGLI LL S M Y+ G+
Sbjct: 26 MILVTYVLKRYVGEVQDSEHFTTASRSVKTGLISSAVVSSWTWPGTLLTSAGMAYEYGVC 85
Query: 88 XXXXXXXXXXXQVILFSALAIKCKQRAPNAHTYLEIIKARYGTIGHFCYMFYALATNVLV 147
Q+ F+ +A++ K+ AP AHT +EI+KAR+G H ++FYAL TN++V
Sbjct: 86 GSMWYSFAFTVQITFFTVIALQVKRVAPGAHTIVEIVKARFGQASHAVFLFYALGTNIIV 145
Query: 148 TAMLLTGGSAVVSDLTGMHTVAACFLLPVGVVLYTIFGGIKATFLTDYVHTXXXXXXXXX 207
+AMLL GGS +S +TGMH VAA FLLP+GV LYT+ GG+K+TFL+D+ HT
Sbjct: 146 SAMLLLGGSQAISAITGMHVVAAGFLLPLGVWLYTVSGGLKSTFLSDWTHTVIVYIVILI 205
Query: 208 FAFTVYATNSQLGSPKAVYDLVREAAKAHPIEGNAGGEYLTMRSRSGGIFFVINIV-GNF 266
F Y ++ +GS +YDL+ E +K +P G G YLT+ +R +F NIV G F
Sbjct: 206 TLFVAYTSSVHIGSIDKMYDLLTEVSKTNPSTGYK-GSYLTVTNRD-AVFVGWNIVIGGF 263
Query: 267 GTVFLDAGYWNKAISSSPAAALPGYVLGGLSWIAIPNLISLAMGLACVALESSPNFPTYP 326
TVF D Y KAI++ P +A+ GY GGL+W+ +P + A L+C+AL ++P TYP
Sbjct: 264 ATVFCDPSYGQKAIAAKPISAMKGYFAGGLAWLIVPWAMGSAAALSCLALTNNPVSVTYP 323
Query: 327 ERLTAEQVSAGLVLPTAAVTLLGKGGAVASLLLVFMAVTSAMSAELIAVSSIFTYDIYRS 386
+ ++++QVS G+ L L+GK GA A +L++FMA TSA SAEL+A SS+ TYD+YR+
Sbjct: 324 DPVSSKQVSEGMPLLYGMTALMGKNGAAAGVLILFMASTSATSAELVAFSSVMTYDVYRN 383
Query: 387 YLKPKATGKQLIFSSHISCIVFGLIMSGFATGLYYAGISMGYLYELMGIIISSAVIPCAL 446
Y +P A+GK+L+ +H+ +F + M A Y GI++ ++ +GI + AV L
Sbjct: 384 YFRPNASGKELVRVTHVFVTIFAVCMGALAVLFNYIGITISWIITFIGIALGPAVFGITL 443
Query: 447 SLFWDAQNLVAVVASPIIGTSLAIMSWL-VCTKSLYGEITVSTTFEDDPMLTGNIVAXXX 505
+LFW N ++ +G+ ++ W+ C K G + +T GN
Sbjct: 444 TLFWKKMNKYGMIIGCPMGSITGVVCWVGSCYKFSNGVVNKTTLNTPYANAVGNFTGLFS 503
Query: 506 XXXXXXXXXYIFKPQNFDWEILKTITRADXXXXXXXXXXXXXXXXXXXXXXXXQDAEKLQ 565
Y F ++ D L
Sbjct: 504 GLIYIVLISYFFPNKSDDLNNLN------------------------------------- 526
Query: 566 AVKTVITTVDEIPRETIERMAEEESQYLSRASKIAGYLAIFFAISFMVLWPMPMYGTGYI 625
+ + D E ++ AE E + L R+ +I +++ +++ P+PMYG+ YI
Sbjct: 527 --EKFVLGDDATAEEIVD--AETEKKQLDRSLRIGIFVSWIIFFILVIIVPLPMYGSKYI 582
Query: 626 FSEKFFTGWVCVLIIWIFFTAFCVCCYPLWEGRHGI 661
FS+ FF GW+ V+IIW A + YPL+E R I
Sbjct: 583 FSKLFFRGWIIVIIIWTLIAALYITFYPLYESRDTI 618
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.324 0.137 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,473,295
Number of Sequences: 60738
Number of extensions: 1280548
Number of successful extensions: 3809
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3796
Number of HSP's gapped (non-prelim): 8
length of query: 723
length of database: 30,389,216
effective HSP length: 114
effective length of query: 609
effective length of database: 23,465,084
effective search space: 14290236156
effective search space used: 14290236156
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)