ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4492 good R KOG3010 General function prediction only Methyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII4492 1591629 1590739 -297
(297 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER175c [R] KOG3010 Methyltransferase 199 5e-51
YHR209w [R] KOG3010 Methyltransferase 119 4e-27
At4g22530 [R] KOG3010 Methyltransferase 74 3e-13
At5g10830 [R] KOG3010 Methyltransferase 66 7e-11
At2g41380 [R] KOG3010 Methyltransferase 62 1e-09
At3g61210 [R] KOG3010 Methyltransferase 53 6e-07
SPAC25B8.09 [R] KOG3010 Methyltransferase 51 2e-06
At3g54150 [R] KOG3010 Methyltransferase 50 5e-06
At1g55450 [R] KOG3010 Methyltransferase 49 9e-06
>YER175c [R] KOG3010 Methyltransferase
Length = 299
Score = 199 bits (505), Expect = 5e-51
Identities = 107/307 (34%), Positives = 169/307 (54%), Gaps = 20/307 (6%)
Query: 1 MSEFTKTDFNAGDYSNFRPSYPPEWYRTLNEFHKGNRNLVIDVGCGPGTATFDISRNLS- 59
MS F+ +DFN+ YS+ RPSYP ++Y+ ++E+H G R L++DVGCGPGTAT +++ L
Sbjct: 1 MSTFSASDFNSERYSSSRPSYPSDFYKMIDEYHDGERKLLVDVGCGPGTATLQMAQELKP 60
Query: 60 FKRVIGADISEPMIEKA---KGRAIEVDNSVKFVVYNGELDDIISRDPFNPSSKQVPDLI 116
F+++IG+D+S MI+ A K + + +V F + + + + D + KQ D+I
Sbjct: 61 FEQIIGSDLSATMIKTAEVIKEGSPDTYKNVSFKISSSDDFKFLGADSVD---KQKIDMI 117
Query: 117 TMAECAHWMDWDTILGQVAKILPSQGTLAVWGYVDPAFVDFPELDDEIEKLQYGDAPFGL 176
T ECAHW D++ L GT+A+WGY DP F D+PE DD + ++ YG GL
Sbjct: 118 TAVECAHWFDFEKFQRSAYANLRKDGTIAIWGYADPIFPDYPEFDDLMIEVPYGKQ--GL 175
Query: 177 GPYWQQPGRQRLRDLYPQLPPSEKWFTDINL-------WRHDIRSQDNKNTPLIMEKTMT 229
GPYW+QPGR RLR++ + F DI + R ++ + PL++ K +T
Sbjct: 176 GPYWEQPGRSRLRNMLKDSHLDPELFHDIQVSYFCAEDVRDKVKLHQHTKKPLLIRKQVT 235
Query: 230 LEAFDKYTTTWSSYHKWQQLHQKQNDGSINAINPRTIFLNKVYKHTGLKSHDLVRVAWKT 289
L F Y TWS+YH+W+Q + ++ + + + L ++ + V W T
Sbjct: 236 LVEFADYVRTWSAYHQWKQDPKNKDKEDV----ADWFIKESLRRRPELSTNTKIEVVWNT 291
Query: 290 VALLARR 296
L +R
Sbjct: 292 FYKLGKR 298
>YHR209w [R] KOG3010 Methyltransferase
Length = 291
Score = 119 bits (299), Expect = 4e-27
Identities = 87/306 (28%), Positives = 146/306 (47%), Gaps = 29/306 (9%)
Query: 2 SEFTKTDFNAGDYSNFRPSYPPEWYRTLNEFHKGNRNLVIDVGCGPGTATFDISRNLSFK 61
+ + +F + Y+N RPSYP + +FHKG R ++D+GCG G ATF + FK
Sbjct: 4 TSYLNKNFESAHYNNVRPSYPLSLVNEIMKFHKGTRKSLVDIGCGTGKATFVVEP--YFK 61
Query: 62 RVIGADISEPMIEKAKGRAIE--VDNSVKFVVYNGELDDIISRDPFNPSSKQVPDLITMA 119
VIG D S M+ A+ E +D ++F+ GE D+ S P + D++ A
Sbjct: 62 EVIGIDPSSAMLSIAEKETNERRLDKKIRFINAPGE--DLSSIRP------ESVDMVISA 113
Query: 120 ECAHWMDWDTILGQVAKILPSQGTLAVWGYVDPAFVDFPELDDEIEKLQYGDAPFGLGPY 179
E HW + + + QV+ IL S GT A W Y+ P FVDFPE + K YG + +G Y
Sbjct: 114 EAIHWCNLERLFQQVSSILRSDGTFAFWFYIQPEFVDFPEALNVYYK--YGWSKDYMGKY 171
Query: 180 WQQPGRQRLRDLYPQLPPS--EKWFTDINLWRH-----DIRSQDNKNTPLIMEKTMTLEA 232
R+ L + + S F DI + + + + +N+ + +TL
Sbjct: 172 LNDNQREILLNYGGEKLRSLLSDRFGDIEVTIYSPSDPNASTVTAENSQFLWRAAITLNQ 231
Query: 233 FDKYTTTWSSYHKWQQLHQKQNDGSINAINPRTIFLNKVYKHTGLKSHDL-VRVAWKTVA 291
F ++ +WS Y W + + + D + IF+N++ + + ++ +++ W T
Sbjct: 232 FKEFVKSWSIYTSWARDNPSKPDIA-------DIFINELKEICHCEDLNVPLKIEWSTFY 284
Query: 292 LLARRK 297
L R++
Sbjct: 285 YLCRKR 290
>At4g22530 [R] KOG3010 Methyltransferase
Length = 261
Score = 73.6 bits (179), Expect = 3e-13
Identities = 65/236 (27%), Positives = 96/236 (40%), Gaps = 26/236 (11%)
Query: 8 DFNAGDYSNFRPSYPPEWYRTLNEFHKGNRNLVIDVGCGPGTATFDISRNLSFKRVIGAD 67
D A Y + RP+YP +WY L NL D G G G A I+ + ++RV+ D
Sbjct: 6 DSQADIYLDARPTYPADWYSKLAAL-SHRHNLAWDAGTGNGQAAIGIAEH--YERVVATD 62
Query: 68 ISEPMIEKAKGRAIEVDNSVKFVVYNGELDDIISRDPFNPSSKQVPDLITMAECAHWMDW 127
+SE M+ K + + E+ D+I + DLIT+A HW D
Sbjct: 63 VSETMLNLGKPHPKVTYHHTPSSMTEDEMVDLI-------GGENSVDLITVATAVHWFDL 115
Query: 128 DTILGQVAKILPSQ-GTLAVWGYVDPAFVDFPELDDEIEKLQYGDAPFGLGPYWQQPGRQ 186
++L G +AVW Y V+ PE D + + PY + P Q
Sbjct: 116 PRFYAIANRLLRKPGGIIAVWSYNTDMVVN-PEFDSVMTRFIAETL-----PYCKFPESQ 169
Query: 187 RLRDLYPQLPPSEKWFTDINLWRHDIRSQDNKNTPLIMEKTMTLEAFDKYTTTWSS 242
D Y LP F + L + K L M+KT++ E F + +WS+
Sbjct: 170 YFLDGYKTLPFP---FESVGL------GSEGKPMELEMKKTVSFEGFLRMLRSWSA 216
>At5g10830 [R] KOG3010 Methyltransferase
Length = 261
Score = 65.9 bits (159), Expect = 7e-11
Identities = 60/236 (25%), Positives = 97/236 (40%), Gaps = 27/236 (11%)
Query: 8 DFNAGDYSNFRPSYPPEWYRTLNEFHKGNRNLVIDVGCGPGTATFDISRNLSFKRVIGAD 67
D A Y + RP+YP EW+ L + + L D G G A ++ + ++ V+ D
Sbjct: 6 DKQADLYLDARPNYPSEWFSKLADL-TDHHGLAWDAATGNGQAALAVAEH--YESVVATD 62
Query: 68 ISEPMIEKAKGRAIEVDNSVKFVVYNGELDDIISRDPFNPSSKQVPDLITMAECAHWMDW 127
+SE ++ A + + EL ++I + DLIT+A+ HW D
Sbjct: 63 VSESQLKLATPHPKINYRHTPTSMTDDELVELI-------GGENSVDLITVAQGVHWFDL 115
Query: 128 DTILGQVAKILPSQ-GTLAVWGYVDPAFVDFPELDDEIEKLQYGDAPFGLGPYWQQPGRQ 186
++L G +AVWGY D + PE D +L PF W+ P Q
Sbjct: 116 PRFYSVATRLLRKPGGIIAVWGYND--VIVSPEFDAVQYRLHATTLPF-----WKYPYIQ 168
Query: 187 RLRDLYPQLPPSEKWFTDINLWRHDIRSQDNKNTPLIMEKTMTLEAFDKYTTTWSS 242
+ D Y LP F ++ + + + L M KT + E + +WS+
Sbjct: 169 HIFDSYEALPFP---FENVGM------GSEGEPLKLEMPKTTSFEGIIRMFKSWSA 215
>At2g41380 [R] KOG3010 Methyltransferase
Length = 269
Score = 61.6 bits (148), Expect = 1e-09
Identities = 64/239 (26%), Positives = 104/239 (42%), Gaps = 33/239 (13%)
Query: 11 AGDYSNFRPSYPPEWYRTLNEFHKGNRNLVIDVGCGPGTATFDISRNLS--FKRVIGADI 68
A Y+ RPSYP + + + +L DVG G G A SR+L+ +K VI D
Sbjct: 9 AEQYAAARPSYPTKLFEYIAS-KTPCHDLAWDVGAGSGQA----SRSLAGIYKNVIATDT 63
Query: 69 SEPMIEKAKGRAIEVDNSVKFVVYNGELDDIISRDPFNPSSKQVPDLITMAECAHWMDWD 128
S +E A + + E++ +++ P S DL+T+A+ HW D
Sbjct: 64 SSKQLEFAAKLPNVRYEITPPTMSSSEIEKLVA-----PESSV--DLVTVAQALHWFDLT 116
Query: 129 TILGQVAKILPS-QGTLAVWGYVDPAFVDFPELDDEIEKLQYGDAPFGLGPYWQQPGRQR 187
V +L G +A W Y + PE++D ++K+ LGP+W R+
Sbjct: 117 NFYSNVKHVLKKPNGVIAAWCYTN------PEVNDAVDKVFQRFYDEKLGPHWDL-ARRL 169
Query: 188 LRDLYPQLP-PSEKWFTDINLWRHDIRSQDNKNTPL--IMEKTMTLEAFDKYTTTWSSY 243
+ D Y + P EK D S ++++ P+ + EK M E + Y + S+Y
Sbjct: 170 VEDGYRGIEFPFEKVDND--------ESTESQSFPVRFVTEKEMVFEEYMTYLRSSSAY 220
>At3g61210 [R] KOG3010 Methyltransferase
Length = 261
Score = 52.8 bits (125), Expect = 6e-07
Identities = 43/169 (25%), Positives = 74/169 (43%), Gaps = 21/169 (12%)
Query: 11 AGDYSNFRPSYPPEWYRTLNEFHKGNRNLVIDVGCGPGTATFDISRNLSFKRVIGADISE 70
A +Y N RP YP WY+ L N + DVG G G A ++ +++V+ DI+E
Sbjct: 12 ADEYLNARPKYPTIWYKVL-AGRTSNHKVAWDVGTGNGQAAIGVAE--YYQKVVATDINE 68
Query: 71 PMIEKAKGRAIEVDNSVKFVVYNGEL----DDIISRDPFNPSSKQVPDLITMAECAHWMD 126
+++A K Y+ DD+++ + D+I A+ H+ D
Sbjct: 69 SQLQRAM-------KHPKVTYYHTPSSMSDDDLVTL----LGGENSIDIIIAAQALHYFD 117
Query: 127 WDTILGQVAKILPSQ-GTLAVWGYVDPAFVDFPELDDEIEKLQYGDAPF 174
V ++L Q G + VW Y D + P++D +++L P+
Sbjct: 118 LKRFYPIVKRVLRKQGGIIVVWVYND--LIITPKVDSIMKRLVDSTLPY 164
>SPAC25B8.09 [R] KOG3010 Methyltransferase
Length = 251
Score = 50.8 bits (120), Expect = 2e-06
Identities = 45/167 (26%), Positives = 69/167 (40%), Gaps = 18/167 (10%)
Query: 6 KTDFNAGDYSNFRPSYPPEWYRTLN-EFHKGNRNLVIDVGCGPGTATFDISRNLSFKRVI 64
KTD DY RP YPP+ LN EF + ++++G G G T I + K +I
Sbjct: 7 KTDI--ADYETARPDYPPQITEWLNDEFSVNETSTILELGAGSGKLTPRIIASQP-KEII 63
Query: 65 GADISEPMIEKAKGRAIEVDNSVKFVVYNGELDDIISRDPFNPSSKQVPDLITMAECAHW 124
D M++ K + VD V G I P + DL+ +C HW
Sbjct: 64 AVDTYVEMLDVLKKKFPNVDCRV------GSAMAI-------PLEDESVDLVACGQCFHW 110
Query: 125 MDWDTILGQVAKILPSQGTLA-VWGYVDPAFVDFPELDDEIEKLQYG 170
+ L ++ ++L G LA +W D + + +EK + G
Sbjct: 111 FANEEALKEIYRVLKPNGKLALIWNIRDNSVPWVEKCSQLLEKCRGG 157
>At3g54150 [R] KOG3010 Methyltransferase
Length = 323
Score = 49.7 bits (117), Expect = 5e-06
Identities = 38/162 (23%), Positives = 71/162 (43%), Gaps = 13/162 (8%)
Query: 14 YSNFRPSYPPEWYRTLNEFHKGNRNLVIDVGCGPGTATFDISRNLSFKRVIGADISEPMI 73
Y + RP YP +W++ + + DVG G G A + + ++ V+ DI+E +
Sbjct: 12 YLDARPRYPIDWFKKI-AARTQDHKFAWDVGTGNGQAAIGLVEH--YENVVATDINEAQL 68
Query: 74 EKAKGRAIEVDNSVKFVVYNGELDDIISRDPFNPSSKQVPDLITMAECAHWMDWDTILGQ 133
++A + + + E+ D++ + DLI A+ H+ D +
Sbjct: 69 QRAIKHSRISYHHTPTTISEDEMVDLL-------GGENSVDLIVAAQAVHFFDLNVFYNV 121
Query: 134 VAKILPSQGTL-AVWGYVDPAFVDFPELDDEIEKLQYGDAPF 174
++L +G L AVW Y D + E+D +++L PF
Sbjct: 122 AKRVLRKEGGLIAVWVYND--IIISHEIDPIMKRLVDSTLPF 161
>At1g55450 [R] KOG3010 Methyltransferase
Length = 311
Score = 48.9 bits (115), Expect = 9e-06
Identities = 43/169 (25%), Positives = 71/169 (41%), Gaps = 13/169 (7%)
Query: 7 TDFNAGDYSNFRPSYPPEWYRTLNEFHKGNRNLVIDVGCGPGTATFDISRNLSFKRVIGA 66
+D A Y N RP YP +W+ L G DVG G G A ++ F++V
Sbjct: 5 SDKLADAYQNARPRYPIDWFTKL-AARTGQHKSAWDVGTGNGQAAIGLAE--YFEKVTAT 61
Query: 67 DISEPMIEKAKGRAIEVDNSVKFVVYNGELDDIISRDPFNPSSKQVPDLITMAECAHWMD 126
DI+E +++A + + ++ ++ D DLI A+ H+ D
Sbjct: 62 DINEAQLKRAVKHERISYHHTPTTMSEDQMVALVGGD-------NSVDLIVAAQAVHYFD 114
Query: 127 WDTILGQVAKILPSQGTL-AVWGYVDPAFVDFPELDDEIEKLQYGDAPF 174
++L +G L AV+ Y D + PE+D +++L PF
Sbjct: 115 LAPFYNVAKRVLRKEGGLIAVFVYND--IIISPEVDSIMKRLVDSTFPF 161
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.137 0.434
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,379,093
Number of Sequences: 60738
Number of extensions: 924607
Number of successful extensions: 1972
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1955
Number of HSP's gapped (non-prelim): 10
length of query: 297
length of database: 30,389,216
effective HSP length: 106
effective length of query: 191
effective length of database: 23,950,988
effective search space: 4574638708
effective search space used: 4574638708
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)