ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4507 good R KOG2382 General function prediction only Predicted alpha/beta hydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII4507 1594915 1595835 307
(307 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR015c [R] KOG2382 Predicted alpha/beta hydrolase 244 1e-64
YGR031w [R] KOG2382 Predicted alpha/beta hydrolase 97 2e-20
SPAC22H12.03 [R] KOG2382 Predicted alpha/beta hydrolase 89 8e-18
7290805 [R] KOG2382 Predicted alpha/beta hydrolase 69 1e-11
CE18906 [R] KOG2382 Predicted alpha/beta hydrolase 62 1e-09
At3g52570 [R] KOG2382 Predicted alpha/beta hydrolase 52 1e-06
>YGR015c [R] KOG2382 Predicted alpha/beta hydrolase
Length = 328
Score = 244 bits (622), Expect = 1e-64
Identities = 138/325 (42%), Positives = 196/325 (59%), Gaps = 28/325 (8%)
Query: 2 MSRRLHS--LAKKDIVDLAYSHIGGKFAGNHL-----HPAIITLHGVFGAKAHFKPLAKR 54
MSR H+ L K IVDL++ + L PAII +HG+ G+ F L K
Sbjct: 1 MSRLAHNKALPYKIIVDLSFHRTRLPSDVSSLIKFEQRPAIINIHGLLGSHVMFHSLNKL 60
Query: 55 LASDLKTDIYSVDLRNHGDSPIAKPYDYITLSKDIVHFIKTQVGAERPVQMIGFSLGGKV 114
L+ L DI+SVD+RNHG SP A PYDY TL+ D+++FI+T +G ERP+ ++GFS+GGK+
Sbjct: 61 LSRKLDADIFSVDVRNHGISPKAIPYDYTTLTNDLIYFIETHIGLERPIYLLGFSMGGKI 120
Query: 115 SLISTLSDECNIRGCVSIDIPPYETPVLDPILLENYDTILKIINGEDGYVIKKGETNWKH 174
+L++TL NIR C+SID+PPYETP LDP++L+NYD I++II + I +G +W+
Sbjct: 121 ALLTTLYKNINIRKCISIDLPPYETPELDPMILQNYDLIMRIIRRD--VKILRGSPSWQK 178
Query: 175 KVLNIFKANKCNWNE---GLARYFAAGFVMNPVNH----------SVKDEFLEFKQPLKL 221
KVL +FK+ +CN + +A YFA GF+ N+ D ++ + PL
Sbjct: 179 KVLELFKSLECNKRKCGGAVALYFANGFLSVKSNNVHQAQLHYEQQQHDPYINYSMPLSS 238
Query: 222 MPDLLDELKAWPD---KEAFGANGFNYQTQKPVLFCKALKSPFISSDYSLLSKQYPNCTV 278
MP+LLDE+K WPD + F G + VLF K L+S FI++DYSLL +P V
Sbjct: 239 MPNLLDEVKKWPDLSNQRDFFQKG---TASRKVLFMKGLQSNFINNDYSLLRYNFPCADV 295
Query: 279 EEFNTSHNIAHEAADRFYESITSFF 303
EFNT HN+ E + ++ I +FF
Sbjct: 296 REFNTGHNLLLENPEDSFKCILNFF 320
>YGR031w [R] KOG2382 Predicted alpha/beta hydrolase
Length = 342
Score = 97.4 bits (241), Expect = 2e-20
Identities = 78/283 (27%), Positives = 133/283 (46%), Gaps = 24/283 (8%)
Query: 33 PAIITLHGVFGAKAHFKPLAKRLASDLKTDIYSVDLRNHGDSPIAKPYDYITLSKDIVHF 92
P II LHG+FG K + + + + L L D+Y +DLRNHG SP + ++Y +S+D+ HF
Sbjct: 75 PPIIILHGLFGNKLNNRSIGRNLNKKLGRDVYLLDLRNHGSSPHSSVHNYEVMSEDVKHF 134
Query: 93 I-KTQVGAERPVQMIGFSLGGKVSLISTLSDE--CNIRGCVSIDIPPYETPVLDPILLEN 149
I K ++ +IG S+GGKV+++ L + C++ C+ + P P + +E
Sbjct: 135 ITKHELNTNGGPIIIGHSMGGKVAMMLVLKNPQLCSMLVCIE-NAPVSLRPNAE--FVEY 191
Query: 150 YDTILKIINGEDGYVIKKGETNWKHKVLNIFKANKCNWNEGLARYFAAGF--VMNPVNHS 207
+++I+N KG+T K + A + NE + R+ V + S
Sbjct: 192 IKALMEIVN-------DKGKTIRTLKQADEHLAERIGGNELVRRFLLTALKKVKMDNSSS 244
Query: 208 VKDEFLEFKQPLKLMPDLL--DELKAWPDKEAFGANGFNYQTQKPVLFCKALKSPFISSD 265
V E + PL + D + E+ AWP A + +P LF +A +S ++ +
Sbjct: 245 VSSYTFEERIPLATLKDAIVKGEIAAWPLDPA------RERWTRPALFIRATQSHYVVDE 298
Query: 266 Y-SLLSKQYPNCTVEEFNTSHNIAHEAADRFYESITSFFKRCE 307
Y ++ +P + + H + E ESI F +R E
Sbjct: 299 YLPIIGAFFPRFETRDIDAGHWVNAEKPGECAESIVDFVERHE 341
>SPAC22H12.03 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 270
Score = 89.0 bits (219), Expect = 8e-18
Identities = 71/273 (26%), Positives = 124/273 (45%), Gaps = 27/273 (9%)
Query: 32 HPAIITLHGVFGAKAHFKPLAKRLASDLKTDIYSVDLRNHGDSPIAKPYDYITLSKDIVH 91
HP ++ HG+ G+K +++ LAK+ + L DIY++D R HGDSP P Y ++ D
Sbjct: 20 HPPVLIFHGLLGSKRNWRSLAKKFSCKLDRDIYAIDQRCHGDSPCVAPLSYSAMALDAFQ 79
Query: 92 FIKTQVGAERPVQMIGFSLGGKVSLISTLSDECNIRGCVSIDIPPYETPVLDPILLENYD 151
F+K +IG S+G K ++++ L + V +D P+ L +Y
Sbjct: 80 FMKDH--KLDKASIIGHSMGAKTAMVTALKWPDKVEKLVVVDNSPWYQD-----LPRDYG 132
Query: 152 TIL-KIINGEDGYVIKKGETNWKHKVLNIFKANKCNWNEGLARYFAAGFVMNPVNHSVKD 210
K+I ++ + K E + K+++ + + L R F + N+S
Sbjct: 133 AYFRKMIQIDEANITKYSEAD---KMMSTVE------KDILVRSFLLSNLKKDSNNS--- 180
Query: 211 EFLEFKQPLKLMPDLLDELKAWPDKEAFGANGFNYQTQKPVLFCKALKSPFI-SSDYSLL 269
+F+ P++L+ L ++ +P N Y + P L +ALK+PFI S +
Sbjct: 181 NTFKFRVPIELISKSLKTIEGFP----ASLNDLVYDS--PTLVIRALKAPFIPDSALPVF 234
Query: 270 SKQYPNCTVEEFNTSHNIAHEAADRFYESITSF 302
K +P + + H + E F ESI +F
Sbjct: 235 KKFFPKYELVSLDCGHWVHFEKPKEFSESIINF 267
>7290805 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 308
Score = 68.6 bits (166), Expect = 1e-11
Identities = 62/313 (19%), Positives = 132/313 (41%), Gaps = 33/313 (10%)
Query: 1 MMSRRLHSLAKKDIVDLAYSHIGGKFAGNHLHPAIITLHGVFGAKAHFKPLAKRLASDLK 60
++ RR +S D V+L++ G+ P ++T HG+FG+K +++ ++K L +
Sbjct: 23 LVVRREYSSEIPDPVELSFDSYTGE--NPETSPPLLTYHGLFGSKQNWRGISKALVRKVS 80
Query: 61 TDIYSVDLRNHGDSPIAKPYDYITLSKDIVHFIKTQVGAERPVQMIGFSLGGKVSLISTL 120
+Y++D+RNHG+SP + ++ +S+D+ F++ + + +G S+GG+ +
Sbjct: 81 RKVYAIDVRNHGESPHSSVHNSKAMSEDLRLFMEQR--SHPNAACMGHSMGGRSMMYFAR 138
Query: 121 SDECNIRGCVSIDIPPYETPVLDPILLENYDTILKIINGEDGYVIKKGETNWKHKVLNIF 180
+ + +DI P P + E +D ++ ++ + +G + K+L
Sbjct: 139 KYPELVERLIVVDISPISVPRSTGEMTEIFDAMVS-LDLSPSMSMSEGRKIAREKLLKAT 197
Query: 181 KANKCNW-NEGLARYFAAGFVMNPVNHSVKDEFL----EFKQPLKLMPDLLDELKAWPDK 235
+ ++ L + G N V EFL +++ L+ +P
Sbjct: 198 EDETVDFIMLNLRKNPDTGAFSWACNAHVLREFLTRFDKYQSNLEELPPYTG-------- 249
Query: 236 EAFGANGFNYQTQKPVLFCKALKSPFISSD-YSLLSKQYPNCTVEEFNTSHNIAHEAADR 294
P F +SP++ + + + K +PN + + H + E
Sbjct: 250 --------------PTTFICGTRSPYMRREQWPQIQKMFPNSEIHWLDAGHLVHFEKPQE 295
Query: 295 FYESITSFFKRCE 307
F ++ F R E
Sbjct: 296 FLTIVSEFLNRTE 308
>CE18906 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 299
Score = 61.6 bits (148), Expect = 1e-09
Identities = 30/83 (36%), Positives = 51/83 (61%), Gaps = 4/83 (4%)
Query: 35 IITLHGVFGAKAHFKPLAKRLASDLKTDIYSVDLRNHGDSPIAKPYDYITLSKDIVHFI- 93
++ +HG+FG K ++ + K L L+ +Y+VD+RNHG SP + Y +++D+V FI
Sbjct: 48 LVIVHGLFGQKQNWNSVGKALHKKLEAPVYAVDVRNHGSSPHTETMSYTEMAEDLVLFID 107
Query: 94 --KTQVGAERPVQMIGFSLGGKV 114
K + R V ++G S+GGK+
Sbjct: 108 KVKEETKKTR-VNLLGHSMGGKI 129
>At3g52570 [R] KOG2382 Predicted alpha/beta hydrolase
Length = 343
Score = 51.6 bits (122), Expect = 1e-06
Identities = 33/91 (36%), Positives = 49/91 (53%), Gaps = 11/91 (12%)
Query: 36 ITLHGVFGAKAHFKPLAKRLASDLKTDIYS------VDLRNHGDSP----IAKPYDYITL 85
+ LHG+ G+ +++ ++ LAS L S VDLRNHG S + P+D +
Sbjct: 57 LILHGLLGSGRNWRSFSRSLASSLSVSSASDWKMILVDLRNHGRSAEVEGLNPPHDLVNS 116
Query: 86 SKDIVHFIKTQVGAERPVQMIGFSLGGKVSL 116
+KD+ +K G P +IG SLGGKV+L
Sbjct: 117 AKDLADLVKAS-GWNWPDVVIGHSLGGKVAL 146
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.138 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,580,468
Number of Sequences: 60738
Number of extensions: 850930
Number of successful extensions: 1875
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1862
Number of HSP's gapped (non-prelim): 6
length of query: 307
length of database: 30,389,216
effective HSP length: 106
effective length of query: 201
effective length of database: 23,950,988
effective search space: 4814148588
effective search space used: 4814148588
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)