ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4559 suspect: LH K KOG2905 Transcription Transcription initiation factor IIF, small subunit (RAP30)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII4559 1608722  1609885 388  
         (388 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YGR005c [K] KOG2905 Transcription initiation factor IIF small su...   433   e-121
SPBC1198.13c [K] KOG2905 Transcription initiation factor IIF sma...    95   2e-19

>YGR005c [K] KOG2905 Transcription initiation factor IIF small subunit
           (RAP30)
          Length = 400

 Score =  433 bits (1114), Expect = e-121
 Identities = 222/336 (66%), Positives = 252/336 (74%), Gaps = 2/336 (0%)

Query: 15  EEAFDGNDIENNEKKVYEESLDLDLSNARKKIWLVRLPKFLAENWRNRTALHGQELGKVR 74
           EE FDGNDIENNE KVYEESLDLDL  + +++WLVRLP FLAE WR+R  LHGQELGK+R
Sbjct: 37  EEVFDGNDIENNETKVYEESLDLDLERSNRQVWLVRLPMFLAEKWRDRNNLHGQELGKIR 96

Query: 75  INNADHSIQLLLNEDEDNKDIPHKYDLELTKKEVTNEYVFTEQNLKKYQQFAKELESNPE 134
           IN     I LLLNE+ DN  IPH+YDLELTKK V NEYVFTEQNLKKYQQ  KELE++PE
Sbjct: 97  INKDGSKITLLLNEN-DNDSIPHEYDLELTKKVVENEYVFTEQNLKKYQQRKKELEADPE 155

Query: 135 LQRQAYIXXXXXXXXXXXXXXXXXXXYNKRRFQHRVMTDRDGRDRYIPYVKTIPKKTAVT 194
            QRQAY+                    N+++F HRVMTDRDGRDRYIPYVKTIPKKTA+ 
Sbjct: 156 KQRQAYLKKQEREEELKKKQQQQKRRNNRKKFNHRVMTDRDGRDRYIPYVKTIPKKTAIV 215

Query: 195 GKVYHECQVMPSIDDPNYHTIVEHRRQIVRNIHKPTVTVLDQTPGVTMSNAGMSMRSDTS 254
           G V HECQVMPS++DPNYH IVE RR IV+  +K  +T LD+T GVTMS+ GMSMRSD S
Sbjct: 216 GTVCHECQVMPSMNDPNYHKIVEQRRNIVKLNNKERITTLDETVGVTMSHTGMSMRSDNS 275

Query: 255 KFLKVGKEK-KNNARAIRLPXXXXXXXXXXXXXXXXXWSLKGLKERTRQPEAYLKESLDQ 313
            FLKVG+EK K+N ++IR+P                 WSLKGLKERTRQPEA+LKE LD+
Sbjct: 276 NFLKVGREKAKSNIKSIRMPKKEILDYLFKLFDEYDYWSLKGLKERTRQPEAHLKECLDK 335

Query: 314 VAMLVKKGPYALKYTLKSEYKKLKEAERAATLGELA 349
           VA LVKKGPYA KYTL+ EYKKLKE ER ATLGELA
Sbjct: 336 VATLVKKGPYAFKYTLRPEYKKLKEEERKATLGELA 371


>SPBC1198.13c [K] KOG2905 Transcription initiation factor IIF small subunit
           (RAP30)
          Length = 307

 Score = 94.7 bits (234), Expect = 2e-19
 Identities = 57/153 (37%), Positives = 80/153 (52%), Gaps = 4/153 (2%)

Query: 189 KKTAVTGKVYHECQVMPSIDDPNYHTIVEHRRQIVRNIHKPTVTVLDQTPGVTMS--NAG 246
           K TA+ G V HEC V P I+D +Y  +++ +R +  +  K  V ++D   G  ++    G
Sbjct: 108 KSTALVGTVAHECNVSPVIND-DYRRVMQ-KRALAASAPKRKVQMIDDRGGSLLAPGTLG 165

Query: 247 MSMRSDTSKFLKVGKEKKNNARAIRLPXXXXXXXXXXXXXXXXXWSLKGLKERTRQPEAY 306
              RS TS    V        +  R+P                 W+LKGL+E  +QPE Y
Sbjct: 166 SRSRSTTSFIRNVKPRTGEGLKNSRIPRNELLDILFKCFEDYEYWTLKGLREYVKQPEVY 225

Query: 307 LKESLDQVAMLVKKGPYALKYTLKSEYKKLKEA 339
           LKE LD +A+L K+GPYALKY+LK EYK   +A
Sbjct: 226 LKEVLDSIAILNKRGPYALKYSLKPEYKGTMDA 258



 Score = 69.3 bits (168), Expect = 9e-12
 Identities = 37/95 (38%), Positives = 51/95 (52%), Gaps = 3/95 (3%)

Query: 23  IENNEKKVYEESL-DLDLSNARKKIWLVRLPKFLAENWRNRTALHGQELGKVRINNADHS 81
           +   E   YE+   DLDL     ++WLV++PKFL + W +        LG VR+ N +  
Sbjct: 8   VRTEEDDRYEDDAGDLDLGQIGSRVWLVKIPKFLMDKWNSIPEDDAANLGCVRVKNDE-- 65

Query: 82  IQLLLNEDEDNKDIPHKYDLELTKKEVTNEYVFTE 116
           IQLLL    +N D+P  Y+L +  K V N YVF E
Sbjct: 66  IQLLLQNSPENADVPKIYNLRVMNKFVRNSYVFRE 100


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.313    0.131    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,988,235
Number of Sequences: 60738
Number of extensions: 762762
Number of successful extensions: 2004
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1996
Number of HSP's gapped (non-prelim): 3
length of query: 388
length of database: 30,389,216
effective HSP length: 108
effective length of query: 280
effective length of database: 23,829,512
effective search space: 6672263360
effective search space used: 6672263360
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)