ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4569 good O KOG1813 Posttranslational modification, protein turnover, chaperones Predicted E3 ubiquitin ligase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII4569 1613311 1613997 229
(229 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR323c [O] KOG1813 Predicted E3 ubiquitin ligase 185 5e-47
Hs5902158 [O] KOG1813 Predicted E3 ubiquitin ligase 135 4e-32
SPBC13E7.02_1 [O] KOG1813 Predicted E3 ubiquitin ligase 135 5e-32
7300590 [O] KOG1813 Predicted E3 ubiquitin ligase 132 5e-31
At1g01350_1 [O] KOG1813 Predicted E3 ubiquitin ligase 126 2e-29
At5g06420 [O] KOG1813 Predicted E3 ubiquitin ligase 126 3e-29
CE26718 [O] KOG1813 Predicted E3 ubiquitin ligase 118 6e-27
ECU06g1060 [O] KOG1813 Predicted E3 ubiquitin ligase 75 8e-14
At4g01020_3 [O] KOG1813 Predicted E3 ubiquitin ligase 59 7e-09
At3g02290 [O] KOG0800 FOG: Predicted E3 ubiquitin ligase 53 3e-07
At3g07200 [O] KOG0320 Predicted E3 ubiquitin ligase 49 4e-06
Hs4504867 [T] KOG3872 FOG: FHA domain 49 8e-06
At5g15790 [O] KOG0800 FOG: Predicted E3 ubiquitin ligase 49 8e-06
>YLR323c [O] KOG1813 Predicted E3 ubiquitin ligase
Length = 259
Score = 185 bits (469), Expect = 5e-47
Identities = 103/256 (40%), Positives = 139/256 (54%), Gaps = 28/256 (10%)
Query: 1 MFKKRVV-KGSRDSKRKRLADEAVASDPVLPKDTGKTXXXXXXXXXXNEDKRALYLAXXX 59
MF+KR+V K S D K ++ + S+ L + + + R L L+
Sbjct: 1 MFRKRLVNKSSSDEKNQKKRQKINFSEEKLVASDEEKGSSDLMSLAKSGNSRTLQLSHEN 60
Query: 60 XXXXXXXXXXXX--XXTVLEQEIDEDI---ERKAKAK----------------VSGFVKP 98
TV + ED+ ERK A+ +SG K
Sbjct: 61 EGKLQKKGEDLDKYTLTVNDDSTKEDLLNFERKELAEKAKKRRPSDDNELVLNMSGKNKR 120
Query: 99 VSK------NMKTVTITDYQPDICKDFQKTGYCGYGDSCKFLHSRDDVAGGWKLNTDWKV 152
++K N++T + D+QPD+CKD+++TGYCGYGDSCKFLHSRDD GWKLN +W
Sbjct: 121 LTKQINQPTNIRTTVLMDFQPDVCKDYKQTGYCGYGDSCKFLHSRDDFKTGWKLNQEWNA 180
Query: 153 DETQEKEVLKELEEIPFRCFLCKKEYTSPVVTKCNHYFCSSCFMKQMKVSTNCPICGKET 212
D+ K V +LE+IPF+C LCK++Y SPVVT C HYFC SCF K MK T C IC KET
Sbjct: 181 DKEDSKAVTLDLEKIPFKCTLCKEDYKSPVVTNCGHYFCGSCFAKDMKKGTKCFICHKET 240
Query: 213 EGAAKMATKLRKLLKK 228
G+AK+A+ L+K+L K
Sbjct: 241 HGSAKVASDLQKMLNK 256
>Hs5902158 [O] KOG1813 Predicted E3 ubiquitin ligase
Length = 343
Score = 135 bits (341), Expect = 4e-32
Identities = 57/133 (42%), Positives = 87/133 (64%), Gaps = 5/133 (3%)
Query: 101 KNMKTVTITDYQPDICKDFQKTGYCGYGDSCKFLHSRDDVAGGWKLNTD-----WKVDET 155
++++ DYQPDICKD+++TG+CG+GDSCKFLH R D GW++ + + V E
Sbjct: 187 EHLRATVRWDYQPDICKDYKETGFCGFGDSCKFLHDRSDYKHGWQIERELDEGRYGVYED 246
Query: 156 QEKEVLKELEEIPFRCFLCKKEYTSPVVTKCNHYFCSSCFMKQMKVSTNCPICGKETEGA 215
+ EV + EEIPF+CF+C++ + +PVVTKC HYFC SC ++ + + C +C ++T G
Sbjct: 247 ENYEVGSDDEEIPFKCFICRQSFQNPVVTKCRHYFCESCALQHFRTTPRCYVCDQQTNGV 306
Query: 216 AKMATKLRKLLKK 228
A +L L+K
Sbjct: 307 FNPAKELIAKLEK 319
>SPBC13E7.02_1 [O] KOG1813 Predicted E3 ubiquitin ligase
Length = 382
Score = 135 bits (340), Expect = 5e-32
Identities = 68/161 (42%), Positives = 95/161 (58%), Gaps = 23/161 (14%)
Query: 82 EDIERKAKAKVSGFVKPV-SKNMKTV---TITDYQPDICKDFQKTGYCGYGDSCKFLHSR 137
+D E+K++ V PV S N TV TI DYQPD+CKD++ TGYCGYGD+CKFLH R
Sbjct: 158 KDFEKKSQ------VGPVLSSNASTVRMNTIIDYQPDVCKDYKLTGYCGYGDTCKFLHMR 211
Query: 138 DDVAGGWKLNTDWKVDETQEKEVL-----------KELEEIPFRCFLCKKEYTSPVVTKC 186
+D GW+L+ +W D QEK ++ E+IPF C +CKK+Y SP+ T C
Sbjct: 212 EDYKAGWQLDREW--DSVQEKYKKGAKLEEGMVKNEKKEDIPFVCLICKKDYRSPIATTC 269
Query: 187 NHYFCSSCFMKQMKVSTNCPICGKETEGAAKMATKLRKLLK 227
H+FC C + + + + C CG +T+G + +LLK
Sbjct: 270 GHHFCEQCAITRYRKTPTCIQCGADTKGLFSVDKNFDRLLK 310
>7300590 [O] KOG1813 Predicted E3 ubiquitin ligase
Length = 357
Score = 132 bits (331), Expect = 5e-31
Identities = 58/127 (45%), Positives = 78/127 (60%), Gaps = 9/127 (7%)
Query: 110 DYQPDICKDFQKTGYCGYGDSCKFLHSRDDVAGGWKLNTDWKVDET---------QEKEV 160
DYQPDICKD+++TGYCG+GDSCKFLH R D GW+L D + T + E+
Sbjct: 194 DYQPDICKDYKETGYCGFGDSCKFLHDRSDYKAGWQLEMDHENQRTGDVDSDGDDTKYEI 253
Query: 161 LKELEEIPFRCFLCKKEYTSPVVTKCNHYFCSSCFMKQMKVSTNCPICGKETEGAAKMAT 220
+ E +PF+C +C++ + +PVVTKC HYFC C + Q K S C IC ++T G A
Sbjct: 254 HSDEETLPFKCHICRQSFVNPVVTKCKHYFCEKCALAQYKKSQRCIICSQQTNGIFNPAK 313
Query: 221 KLRKLLK 227
+L LK
Sbjct: 314 ELIARLK 320
>At1g01350_1 [O] KOG1813 Predicted E3 ubiquitin ligase
Length = 357
Score = 126 bits (317), Expect = 2e-29
Identities = 53/131 (40%), Positives = 77/131 (58%), Gaps = 16/131 (12%)
Query: 110 DYQPDICKDFQKTGYCGYGDSCKFLHSRDDVAGGWKLNTDWK----------------VD 153
DYQPDICKD+++TGYCGYGDSCKFLH R D GW++ +W+ D
Sbjct: 200 DYQPDICKDYKETGYCGYGDSCKFLHDRGDYKPGWQIEKEWEEAEKVRKRNKAMGVEDED 259
Query: 154 ETQEKEVLKELEEIPFRCFLCKKEYTSPVVTKCNHYFCSSCFMKQMKVSTNCPICGKETE 213
+ +K+ ++ +PF CF+C++ + PVVTKC HYFC C +K + C +C + T
Sbjct: 260 DEADKDSDEDENALPFACFICREPFVDPVVTKCKHYFCEHCALKHHTKNKKCFVCNQPTM 319
Query: 214 GAAKMATKLRK 224
G A +++K
Sbjct: 320 GIFNAAHEIKK 330
>At5g06420 [O] KOG1813 Predicted E3 ubiquitin ligase
Length = 378
Score = 126 bits (316), Expect = 3e-29
Identities = 53/131 (40%), Positives = 77/131 (58%), Gaps = 16/131 (12%)
Query: 110 DYQPDICKDFQKTGYCGYGDSCKFLHSRDDVAGGWKLNTDWKV----------------D 153
DYQPDICKD+++TGYCGYGDSCKFLH R D GW++ +W+ D
Sbjct: 235 DYQPDICKDYKETGYCGYGDSCKFLHDRGDYKPGWQIEKEWEEAEKVRKRNKAMGVEDDD 294
Query: 154 ETQEKEVLKELEEIPFRCFLCKKEYTSPVVTKCNHYFCSSCFMKQMKVSTNCPICGKETE 213
+ +K+ ++ +PF CF+C++ + PVVTKC HYFC C +K + C +C + T
Sbjct: 295 DEADKDSDEDENALPFACFICREPFLDPVVTKCKHYFCEHCALKHHTKNKKCFVCNQPTM 354
Query: 214 GAAKMATKLRK 224
G A +++K
Sbjct: 355 GIFNAAHEIKK 365
>CE26718 [O] KOG1813 Predicted E3 ubiquitin ligase
Length = 384
Score = 118 bits (296), Expect = 6e-27
Identities = 48/124 (38%), Positives = 76/124 (60%), Gaps = 5/124 (4%)
Query: 110 DYQPDICKDFQKTGYCGYGDSCKFLHSRDDVAGGWKLNTDWKV-----DETQEKEVLKEL 164
D+ PDICKD+++TG+C +GDSCKF+H R D GW+++ +++ ++ E+ +
Sbjct: 175 DFAPDICKDYKETGFCTFGDSCKFVHDRSDYKHGWEIDEEYEAGKYGAEDDANYEIHEGD 234
Query: 165 EEIPFRCFLCKKEYTSPVVTKCNHYFCSSCFMKQMKVSTNCPICGKETEGAAKMATKLRK 224
+ P CF+C + P+VTKC HYFC+ C +K + S+ CPIC + TE A +L
Sbjct: 235 DTFPEDCFICGNPFVDPIVTKCKHYFCTGCALKSFQKSSKCPICQQNTENIMNTAKELLT 294
Query: 225 LLKK 228
LK+
Sbjct: 295 YLKR 298
>ECU06g1060 [O] KOG1813 Predicted E3 ubiquitin ligase
Length = 101
Score = 75.1 bits (183), Expect = 8e-14
Identities = 37/107 (34%), Positives = 53/107 (48%), Gaps = 17/107 (15%)
Query: 108 ITDYQPDICKDFQKTGYCGYGDSCKFLHSRDDVAGGWKLNTDWKVDETQEKEVLKELEEI 167
+ D +CK F++TGYCGYGDSCK+ H R E +E V+ +
Sbjct: 7 VIDTHKVVCKPFRETGYCGYGDSCKYSHDRS--------------AEYEEAPVISGPGPL 52
Query: 168 PFRCFLCKKEYTSPVVTKCNHYFCSSCFMKQMKVSTNCPICGKETEG 214
C +CKK + VV +C H FCS C +++ + C +CGK G
Sbjct: 53 ---CGICKKTFEERVVAECGHSFCSLCAIRKYQDGDECGVCGKAMYG 96
>At4g01020_3 [O] KOG1813 Predicted E3 ubiquitin ligase
Length = 328
Score = 58.5 bits (140), Expect = 7e-09
Identities = 25/84 (29%), Positives = 44/84 (51%), Gaps = 6/84 (7%)
Query: 145 KLNTDWKVDETQEKEVLKELEEIPFRCFLCKKEYTSPVVTKCNHYFCSSCFMKQMKVSTN 204
KL TD+ + ++ + L P C +C+ + PVVT CNHYFC C +K +
Sbjct: 65 KLETDFVLTGEEDDDAL------PLACSICQNPFLDPVVTNCNHYFCDKCALKHHTENDT 118
Query: 205 CPICGKETEGAAKMATKLRKLLKK 228
C +C + T G A ++++ +++
Sbjct: 119 CFVCNEPTLGLFDTAVEIKERIEE 142
>At3g02290 [O] KOG0800 FOG: Predicted E3 ubiquitin ligase
Length = 67
Score = 53.1 bits (126), Expect = 3e-07
Identities = 26/63 (41%), Positives = 39/63 (61%), Gaps = 6/63 (9%)
Query: 154 ETQEKEVLKELEEIPFRCFLCKKEYTSP---VVTKCNHYFCSSCFMKQMKVSTNCPICGK 210
+++ +L E E++ C C +EYTS +VTKC+H+F SC + M+ S NCP+CGK
Sbjct: 3 DSENMYILSEDEDV---CPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVCGK 59
Query: 211 ETE 213
E
Sbjct: 60 VME 62
>At3g07200 [O] KOG0320 Predicted E3 ubiquitin ligase
Length = 182
Score = 49.3 bits (116), Expect = 4e-06
Identities = 20/56 (35%), Positives = 27/56 (47%)
Query: 156 QEKEVLKELEEIPFRCFLCKKEYTSPVVTKCNHYFCSSCFMKQMKVSTNCPICGKE 211
+ K V +EE F C +C +T V TKC H FC C + + CP C K+
Sbjct: 112 KSKAVAPPVEEPKFSCPICLCPFTQEVSTKCGHIFCKKCIKNALSLQAKCPTCRKK 167
>Hs4504867 [T] KOG3872 FOG: FHA domain
Length = 485
Score = 48.5 bits (114), Expect = 8e-06
Identities = 20/61 (32%), Positives = 32/61 (51%), Gaps = 3/61 (4%)
Query: 154 ETQEKEVLKELEEI---PFRCFLCKKEYTSPVVTKCNHYFCSSCFMKQMKVSTNCPICGK 210
+ Q++EVL + ++ +C +C + + V C H FCS C + MK CPIC K
Sbjct: 383 QAQKEEVLSHMNDVLENELQCIICSEYFIEAVTLNCAHSFCSYCINEWMKRKIECPICRK 442
Query: 211 E 211
+
Sbjct: 443 D 443
>At5g15790 [O] KOG0800 FOG: Predicted E3 ubiquitin ligase
Length = 232
Score = 48.5 bits (114), Expect = 8e-06
Identities = 22/46 (47%), Positives = 29/46 (62%), Gaps = 3/46 (6%)
Query: 171 CFLCKKEYTSP---VVTKCNHYFCSSCFMKQMKVSTNCPICGKETE 213
C C +EY S +VTKC+H+F SC + M+ S NCP+CGK E
Sbjct: 182 CPTCLEEYISENPKIVTKCSHHFHLSCIYEWMERSENCPVCGKVME 227
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.133 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,505,213
Number of Sequences: 60738
Number of extensions: 506966
Number of successful extensions: 2818
Number of sequences better than 1.0e-05: 13
Number of HSP's better than 0.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2802
Number of HSP's gapped (non-prelim): 13
length of query: 229
length of database: 30,389,216
effective HSP length: 103
effective length of query: 126
effective length of database: 24,133,202
effective search space: 3040783452
effective search space used: 3040783452
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)