ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4602 good A KOG2771 RNA processing and modification Subunit of tRNA-specific adenosine-34 deaminase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII4602 1627733 1628716 328
(328 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR316c [A] KOG2771 Subunit of tRNA-specific adenosine-34 deaminase 300 1e-81
SPAP27G11.04c [A] KOG2771 Subunit of tRNA-specific adenosine-34 ... 94 2e-19
Hs19923945 [A] KOG2771 Subunit of tRNA-specific adenosine-34 dea... 72 8e-13
7302591 [A] KOG2771 Subunit of tRNA-specific adenosine-34 deaminase 67 3e-11
CE22043_1 [A] KOG2771 Subunit of tRNA-specific adenosine-34 deam... 64 3e-10
At5g24670 [A] KOG2771 Subunit of tRNA-specific adenosine-34 deam... 54 4e-07
ECU09g0990 [A] KOG2771 Subunit of tRNA-specific adenosine-34 dea... 52 9e-07
>YLR316c [A] KOG2771 Subunit of tRNA-specific adenosine-34 deaminase
Length = 322
Score = 300 bits (769), Expect = 1e-81
Identities = 164/335 (48%), Positives = 218/335 (64%), Gaps = 22/335 (6%)
Query: 1 MVKKHLNPTKIDFIKCIVEDCLWQIRDTKNVDVPDLVEAWSIEVRPRESKSVIPFVRG-I 59
MVKK NP KID+ I+E+ L QIR+ K+V+ P L+ WSI + PR+SK VI +R
Sbjct: 1 MVKKVNNPLKIDYQNGIIENRLLQIRNFKDVNTPKLINVWSIRIDPRDSKKVIELIRNDF 60
Query: 60 QDNDPVSFLHVKRIKK-LDSDTLRVLIGSKEYIPDRSTFDNMLKGV---VFQYDNVKDDN 115
Q NDPVS H+KRI+K +++ TL V++ SKEYI D +N LK + +Y+ + DD
Sbjct: 61 QKNDPVSLRHLKRIRKDIETSTLEVVLCSKEYICDEGEINNKLKSIWVGTKKYE-LSDDI 119
Query: 116 KVPKVGPSTKSLMLKWSEQYWPLIWRGNPNDQILNDYIFDMTEIRSLLTKIAEESRAIRD 175
+VP+ PSTK L WS +YWPLIW GNPNDQILNDY DM E+R+ L++ + S +
Sbjct: 120 EVPEFAPSTKELNNAWSVKYWPLIWNGNPNDQILNDYKIDMQEVRNELSRASTLSVKMAT 179
Query: 176 SDSMQLPIVSAFVNPKSGKI-IISKDYRH--SGSPLDHSVMVGINEVAKEEQERRDRVQN 232
+ Q P+VS FV+P K ++++D R+ + P+DHSVMVGI V + +E D N
Sbjct: 180 AGK-QFPMVSVFVDPSRKKDKVVAEDGRNCENSLPIDHSVMVGIRAVGERLREGVDEDAN 238
Query: 233 GTASEKDIHQGETYLCLDFDVYTSHEPCSMCSMALIHSRVKRCIFINPMSVSGALKPDSG 292
+YLCLD+DVY +HEPCSMCSMALIHSRV+R +F+ M +G+LK SG
Sbjct: 239 ------------SYLCLDYDVYLTHEPCSMCSMALIHSRVRRVVFLTEMQRTGSLKLTSG 286
Query: 293 DGYCMHSNKDLNSKYEVFQWVGEELEVPGIDSTVC 327
DGYCM+ NK LNS YE FQW+GEE V +D VC
Sbjct: 287 DGYCMNDNKQLNSTYEAFQWIGEEYPVGQVDRDVC 321
>SPAP27G11.04c [A] KOG2771 Subunit of tRNA-specific adenosine-34 deaminase
Length = 315
Score = 94.4 bits (233), Expect = 2e-19
Identities = 80/303 (26%), Positives = 136/303 (44%), Gaps = 46/303 (15%)
Query: 40 WSIEVRPRESKSVIPFVRGIQDNDPVSFLHVKRIKKLDSDT----LRVLIGSKEYIPDRS 95
W P+ + V +V+ I+ S LH R++++ + L+++I P++S
Sbjct: 41 WIACFEPKYASKVTQYVKQIRSKQKESLLHCNRLRRIQDENGSLELQIII-----CPEKS 95
Query: 96 TFDN----------MLKGVVFQYDNVKDDNKVPKVGPSTKSLMLKWSEQYWPLIWRGNPN 145
N ++ ++F Y VP P T +W+ WP+ +R +
Sbjct: 96 MTANEIGKDFEDLGIVSKMIFLY-------AVPAFPPLTDEQFHEWNS-VWPVSYRKHVQ 147
Query: 146 DQILNDYIFDMTEIRSLLTKIAEESRAIRDSDSMQLPIVSAFVNPKSGKII-ISKDYRHS 204
Q + + ++ I S+L + + A ++ +A +P + ++ +S D R
Sbjct: 148 RQDVFT-VHELKRIESILEDLINAAGASHKHG--EIGCAAAIYDPTTDTVLAVSVDERSK 204
Query: 205 -GSPLDHSVMVGINEVAKEEQERRDRVQNGTASEKDIHQGETYLCLDFDVYTSHEPCSMC 263
+P++H VM IN VAK E RR QN T KD YLC D V +HEPC MC
Sbjct: 205 LKNPINHCVMNAINLVAKRELSRR---QNRTDGSKD-----RYLCKDLTVVMTHEPCVMC 256
Query: 264 SMALIHSRVKRCIFINPMSVSGALKPDSGDGYCMHSNKDLNSKYEVFQWVGEELEVPGID 323
SM L+HSR++R I+ ++G ++ Y +H +LN +Y + G VP I
Sbjct: 257 SMGLLHSRIRRLIYCKKQPLTGGIE----SLYGIHWRAELNHRYLAYS--GWNKPVPSIK 310
Query: 324 STV 326
+
Sbjct: 311 ENI 313
>Hs19923945 [A] KOG2771 Subunit of tRNA-specific adenosine-34 deaminase
Length = 351
Score = 72.4 bits (176), Expect = 8e-13
Identities = 61/221 (27%), Positives = 105/221 (46%), Gaps = 27/221 (12%)
Query: 117 VPKVGPSTKSLMLKWSEQYWPLIWRGNPN-DQILNDYIFDMTEIRSLLTKIAEESRAIRD 175
VP P T+ + + +WP + + L +F E ++ + + A R
Sbjct: 128 VPARPPLTRG-QFEEARAHWPTSFHEDKQVTSALAGRLFSTQERAAMQSHMERAVWAARR 186
Query: 176 SDSMQLPIVSAFV-NPKSGKIIIS-KDYRHSGSPLDHSVMVGINEVAKEEQERR------ 227
+ + L V A V +P S +++ + D + +PL H+VMV ++ VA+ +
Sbjct: 187 AAARGLRAVGAVVVDPASDRVLATGHDCSCADNPLLHAVMVCVDLVARGQGRGTYDFRPF 246
Query: 228 -----------DRVQNGTASEKDIHQ-GETYLCLDFDVYTSHEPCSMCSMALIHSRVKRC 275
V+ G + D + G YLC +D+Y + EPC+MC+MAL+H+R+ R
Sbjct: 247 PACSFAPAAAPQAVRAGAVRKLDADEDGLPYLCTGYDLYVTREPCAMCAMALVHARILR- 305
Query: 276 IFINPMSVSGALKPDSGDGYCMHSNKDLNSKYEVFQWVGEE 316
+F S GAL G + +H+ DLN +++VF+ V EE
Sbjct: 306 VFYGAPSPDGAL----GTRFRIHARPDLNHRFQVFRGVLEE 342
>7302591 [A] KOG2771 Subunit of tRNA-specific adenosine-34 deaminase
Length = 360
Score = 67.4 bits (163), Expect = 3e-11
Identities = 35/73 (47%), Positives = 46/73 (62%), Gaps = 5/73 (6%)
Query: 246 YLCLDFDVYTSHEPCSMCSMALIHSRVKRCIFINPMSVSGALKPDSGDGYCMHSNKDLNS 305
YLC +D+Y EPC MCSMAL+HSR KR F+ S +GAL + +HS ++LN
Sbjct: 290 YLCTGYDIYLLQEPCLMCSMALVHSRAKRVFFVK-NSENGAL----ATRFQLHSVRELNH 344
Query: 306 KYEVFQWVGEELE 318
YEVFQ+ +E E
Sbjct: 345 HYEVFQFTTKEDE 357
>CE22043_1 [A] KOG2771 Subunit of tRNA-specific adenosine-34 deaminase
Length = 494
Score = 63.9 bits (154), Expect = 3e-10
Identities = 73/288 (25%), Positives = 114/288 (39%), Gaps = 59/288 (20%)
Query: 35 DLVEAWSIEVRPRESKSVIPFVRGIQDNDPVSFLHVKRIKKLDSDTLRVLIGSKEYIPDR 94
D + + S + E+K +I + P H+KR+ K S VLI E +
Sbjct: 31 DTIPSTSFQALRLENKKIIGTIIRRLPELPEELSHLKRVGKDGS----VLIAESEEVA-- 84
Query: 95 STFDNMLKGVVFQYDNVKDDN---KVPKVGPSTKSLMLKWSEQYWPLIWRGNPNDQILND 151
++ G + + +D KV + P T+ + +++ WP + N + L D
Sbjct: 85 ----KLIVGKLEDAEARVEDLVPVKVARTKPQTRR-QFQVAKELWPTGFHPNHELEQLLD 139
Query: 152 YIFDMTEIRSLLTKIAEESRAIRDSDSMQLPIVSAFVNPKSGKIIISKDY-----RHSGS 206
F ++ + + E+ + D G I + D R S
Sbjct: 140 GSFLTESLKKYVDRWLGEAARLGD-----------------GCIAVQNDELLSTGRPSSH 182
Query: 207 PLDHSVMVGINEVAKEEQERRDRVQNGTASEKDIHQGETYLCLDFDVYTSHEPCSMCSMA 266
PL H VM + + K G+ YL DV+ EPC+MCSMA
Sbjct: 183 PLGHPVMEMVGNLPKRH-------------------GDDYLGTGSDVFLITEPCAMCSMA 223
Query: 267 LIHSRVKRCIFINPMSVSGALKPDSGDGYCMHSNKDLNSKYEVFQWVG 314
L+H RVKR +F S +G LK DG+ +H +N YEVF+ G
Sbjct: 224 LVHFRVKR-VFYARNSRNGVLKE---DGWQLHLEPSINHHYEVFRVEG 267
>At5g24670 [A] KOG2771 Subunit of tRNA-specific adenosine-34 deaminase
Length = 438
Score = 53.5 bits (127), Expect = 4e-07
Identities = 34/113 (30%), Positives = 55/113 (48%), Gaps = 14/113 (12%)
Query: 209 DHSVMVGINEVAKEEQ--ERRDRVQNGTASE--KDIHQGETYLCLDFDVYTSHEPCSM-- 262
DH + AK+++ + VQN + E +D YLC +D++ EPC++
Sbjct: 316 DHVPPSNTDSPAKKQKTSSQSPDVQNDSREETVRDPSMERPYLCTGYDIFLLLEPCTIRD 375
Query: 263 ----CSMALIHSRVKRCIFINPMSVSGALKPDSGDGYCMHSNKDLNSKYEVFQ 311
C+MAL+H R+KR + P + +G L G + + K LN Y VF+
Sbjct: 376 MFPRCAMALVHQRIKRIFYAFPNTTAGGL----GSVHRLQGEKSLNHHYAVFR 424
>ECU09g0990 [A] KOG2771 Subunit of tRNA-specific adenosine-34 deaminase
Length = 302
Score = 52.4 bits (124), Expect = 9e-07
Identities = 63/256 (24%), Positives = 104/256 (40%), Gaps = 62/256 (24%)
Query: 69 HVKRIKKLDSDTLRVLIGSKEYIPDRSTFDNMLKGVVFQYDNVKDDN------------- 115
H+KRIK DSD L VL+ + + D + D ++ G+V N
Sbjct: 86 HLKRIKPQDSDLL-VLV---DLVQDGGSMDRVV-GLVRSMAEGGFGNGLPAEDLASSIEI 140
Query: 116 -KVPKVGPSTKSLMLKWSEQYWPLIWRGNPNDQILNDYIFDMTEIRSLLTKIAEESRAIR 174
VP+ P T S + + WP I +++ +YI + E+ ++
Sbjct: 141 VSVPEHQPITDSQYAEGTA-LWPCIRTHRFIEELDIEYITRVVEM-------------LK 186
Query: 175 DSDSMQLPIVSAFVNPKSGKIIISKDYRHSGSPLDHSVMVGINEVAKEEQERRDRVQNGT 234
++ + + + G + + +D + L HSV+ + EV+K +
Sbjct: 187 TRSAISICCGACIIAGGEGIVSVQED---TDDVLGHSVLRAVEEVSKAQV---------- 233
Query: 235 ASEKDIHQGETYLCLDFDVYTSHEPCSMCSMALIHSRVKRCIFINPMSVSGALKPDSGDG 294
+YLC D + EPC CSMA +H R+KR + VSG P S G
Sbjct: 234 ----------SYLCTGLDAFIPREPCLSCSMAFVHGRIKRVFCVK--RVSGG--PFS--G 277
Query: 295 YCMHSNKDLNSKYEVF 310
++ NK LN +Y V+
Sbjct: 278 LKINYNKSLNHRYPVY 293
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.136 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,305,358
Number of Sequences: 60738
Number of extensions: 939587
Number of successful extensions: 2280
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2265
Number of HSP's gapped (non-prelim): 8
length of query: 328
length of database: 30,389,216
effective HSP length: 107
effective length of query: 221
effective length of database: 23,890,250
effective search space: 5279745250
effective search space used: 5279745250
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)