ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4624 suspect: LH U KOG2079 Intracellular trafficking, secretion, and vesicular transport Vacuolar assembly/sorting protein VPS8
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII4624 1632791 1636384 1198
(1198 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YAL002w [U] KOG2079 Vacuolar assembly/sorting protein VPS8 531 e-150
7295341 [U] KOG2079 Vacuolar assembly/sorting protein VPS8 55 7e-07
>YAL002w [U] KOG2079 Vacuolar assembly/sorting protein VPS8
Length = 1176
Score = 531 bits (1367), Expect = e-150
Identities = 355/1188 (29%), Positives = 602/1188 (49%), Gaps = 71/1188 (5%)
Query: 64 IGTAKGAVVIFNKKQFVQSVLAPSLPD------FRSKVTFIDINQDGTHVCAAYESGDIF 117
+GT+KG V+IFN + +Q++L P+L + RS V I I DGTHV A+YE+G+I
Sbjct: 1 MGTSKGCVLIFNYNEHLQTILVPTLSEDPSIHSIRSPVKSIVICSDGTHVAASYETGNIC 60
Query: 118 LWDLNS--QVDENSDSNH-------LEAVLHIASHRGHSFNGLNFIPGKHTNIAVSDSTG 168
+W+LN +V S+ + L AVLHI H GL+F +HT + VSD TG
Sbjct: 61 IWNLNVGYRVKPTSEPTNGMTPTPALPAVLHIDDHVNKEITGLDFFGARHTALIVSDRTG 120
Query: 169 HIVLHKGTRNHLWQLVPSAKEILRLNPMINPLLTTRIRSN-GEDYDAEHLLAVLSNRSFA 227
+ L+ G R WQLV ++K+IL +N L+ +++ + + +LL+VL+ FA
Sbjct: 121 KVSLYNGYRRGFWQLVYNSKKILDVNSSKEKLIRSKLSPLISREKISTNLLSVLTTTHFA 180
Query: 228 VISLSSP-TLSTYRLIELSHKNSATFGY-ISWNDVNPNQVAFSVDNQLSLV-----DIRF 280
+I LS +L +E S +NS ISW N ++VA+SV+N++S++ D
Sbjct: 181 LILLSPHVSLMFQETVEPSVQNSLVVNSSISWTQ-NCSRVAYSVNNKISVISISSSDFNV 239
Query: 281 PTLQWTYEAPECICKVAWXXXXXXXXXXXXXXXXXXXXGSVDTDEDPVLTSIDLLIHELM 340
+ + E E I + W + +L +D LIH+LM
Sbjct: 240 QSASHSPEFAESILSIQWIDQLLLGVLTISHQFLVLH----PQHDFKILLRLDFLIHDLM 295
Query: 341 VPESENHFATLNNTIVVLTNYSLMVGKINSWSDIVLHHIHKGDYINALGTLGYYLQGPSF 400
+P ++ +F + +LTNYS +GK SWSDI L HI KGDY+ AL + LQ +
Sbjct: 296 IPPNK-YFVISRRSFYLLTNYSFKIGKFVSWSDITLRHILKGDYLGALEFIESLLQ--PY 352
Query: 401 DSITALLHLEKDYQKRIRQIEPSFNNLTLATVQFLLKKDDISYSKVTEATSLILDL---- 456
+ LL L+ + ++R +Q+ F NL+LA ++FL+KKD+ Y++V + +++ +
Sbjct: 353 CPLANLLKLDNNTEERTKQLMEPFYNLSLAALRFLIKKDNADYNRVYQLLMVVVRVLQQS 412
Query: 457 -GSIFSDEKMYVLLEQVGEALMTKHNEEFQEILYEKIVNGTVTYLSPILFKAVMEYTVSS 515
+ S + V LEQ E K N + E++ + G+VT +SP+LF+++++Y
Sbjct: 413 SKKLDSIPSLDVFLEQGLEFFELKDNAVYFEVVANIVAQGSVTSISPVLFRSIIDYYAKE 472
Query: 516 GNDRRLEXXXXXXXXXXXXXXYAFKVCKEFRLYSALLYLWTTILDDYKSPLVDIIFYFAG 575
N + +E A K+C+++ L+ L+Y+W I DDY++P+VD+I+ +
Sbjct: 473 ENLKVIEDLIIMLNPTTLDVDLAVKLCQKYNLFDLLIYIWNKIFDDYQTPVVDLIYRISN 532
Query: 576 KEECCVVFDAN--IEDNTLFEYIESTLLGKVYPTEKRV-PSKKILYLKNXXXXXXXXXXX 632
+ E CV+F+ + T+F+Y+ L G+ YP + PS K ++
Sbjct: 533 QSEKCVIFNGPQVPPETTIFDYVTYILTGRQYPQNLSISPSDKCSKIQRELSAFIFSGFS 592
Query: 633 XEWPQGSGKKLLTCPLPAEEPAFPYASLLISSSAQNFLHVLDVSFEDVLFNE------SP 686
+WP S KL C P EEPAFPY LL+ S+ FL +L+ FE LFN+ S
Sbjct: 593 IKWPSNSNHKLYICENPEEEPAFPYFHLLLKSNPSRFLAMLNEVFEASLFNDDNDMVASV 652
Query: 687 QNGFHFTRQHIIDVLLDIQKNF-KENHVKLLIALFVFNNLPKYSQFIRLTNTIIDSQIDV 745
+RQ++ID+LLD K+ +++++L+A+F+ ++ KY QFI+++N +D ++
Sbjct: 653 GEAELVSRQYVIDLLLDAMKDTGNSDNIRVLVAIFIATSISKYPQFIKVSNQALDCVVNT 712
Query: 746 ILSADLEGFEQISEDCLEALLSVYSPDNINALIDKMSGVDFYRVRLLLYKKAGRYTDYLT 805
I S+ ++G +IS+ LE+LL Y I ++ +F +V +YK +Y L+
Sbjct: 713 ICSSRVQGIYEISQIALESLLPYYHSRTTENFILELKEKNFNKVLFHIYKSENKYASALS 772
Query: 806 FILSDQHRNTNGNDIIAGLKLCDGATAKSPIGRSE---ICDLLSDTFPKIIENCQDQRSL 862
IL + N I + + D K P G E + +++ F ++ ++
Sbjct: 773 LILETKDIEKEYNTDI--VSITDYILKKCPPGSLECGKVTEVIETNFDLLLSRIGIEK-C 829
Query: 863 VSIIDSFDSSLHEKILLISDPLTLLGYLRILLSGNYHLSAPFLKKARPKYLSLLYETGDA 922
V+I FD +LH++IL + + T YL L S +++ K+ R ++ L +
Sbjct: 830 VTIFSDFDYNLHQEILEVKNEETQQKYLDKLFS-TPNINNKVDKRLRNLHIELNCKYKSK 888
Query: 923 SGVSQWVDNIDLKNIEVESIVDIINGNRDSRTLIRLLVRLKNYGSAIDKINSKINELLQN 982
+ W++ L N E I+D++N + + + RL+++ A+ + S I + L N
Sbjct: 889 REMILWLNGTVLSNAESLQILDLLNQDSNFEAAAIIHERLESFNLAVRDLLSFIEQCL-N 947
Query: 983 NDFDEIDNYLDLGISVSQKTDETNKN-------WVKLISNLVS---QYPTLTDDQKAFCD 1032
I L+ S+ + D+ N W+ LI+ L++ +YP+ D++K C+
Sbjct: 948 EGKTNISTLLE---SLRRAFDDCNSAGTEKKSCWILLITFLITLYGKYPS-HDERKDLCN 1003
Query: 1033 KSLQKVFIELSDGNLNDIENSEESFFWKTLMEIFENXXXXXXXXXXXXXXXXNIFTAYSV 1092
K LQ+ F+ L + ++S FW+ + + E+ N+F Y +
Sbjct: 1004 KLLQEAFLGLVRSKSSSQKDSGGE-FWEIMSSVLEHQDVILMKVQDLKQLLLNVFNTYKL 1062
Query: 1093 EETVKLLILQIINQSASSDITTYMHRKEHGWAIQSHECEVCGKKIWGIGIDNKVFEQWES 1152
E ++ LI +II S+ + Y GW+I + +CE+CGKKIWG G+D +F WE+
Sbjct: 1063 ERSLSELIQKIIEDSSQDLVQQYRKFLSEGWSIHTDDCEICGKKIWGAGLDPLLFLAWEN 1122
Query: 1153 ARCHE--VAASGSSALVVFSCGHSFHSSCLENMGQHEGAFKCLTCDDE 1198
+ H+ ++ + LV+F C H FH +CLEN+ Q + CL C E
Sbjct: 1123 VQRHQDMISVDLKTPLVIFKCHHGFHQTCLENLAQKPDEYSCLICQTE 1170
>7295341 [U] KOG2079 Vacuolar assembly/sorting protein VPS8
Length = 1229
Score = 55.1 bits (131), Expect = 7e-07
Identities = 55/238 (23%), Positives = 103/238 (43%), Gaps = 26/238 (10%)
Query: 467 VLLEQVGEALMTKHNEEFQEILYEKIVNGTVTYLSPILFKAVMEYTVSSGNDRRLEXXXX 526
+L Q+ E L E F + + E I T+ ++P++ +A+++Y + + +LE
Sbjct: 538 LLWTQLWEKLHNSSTELFLQHISEHIEKETIHSVNPVISQALVDYWLEH-SPAKLEQLIL 596
Query: 527 XXXXXXXXXXYAFKVCKEFRLYSALLYLWTTILDDYKSPLVDIIFYFAGKEECCVVFDAN 586
K K+ RL+ A +YL T L+DY + L +++ V D
Sbjct: 597 KLDWMCLDLNQVLKAVKKHRLFRAQIYLNTQALNDYTAALTELL--------PLVTPDET 648
Query: 587 IEDNTLFEYIESTLLGKVYPTEKRVPSKKILYLKNXXXXXXXXXXXXEWPQGSGKKLLTC 646
N L Y+ S L G+ YP+ + +P + + +K+ E
Sbjct: 649 DLGNCLLVYVSSCLAGREYPSGE-IPVELVHQVKHDVLRCLTSQHSKE------------ 695
Query: 647 PLPAEEPAFPYASLLISSSAQNFLHVLDVSFEDVLFNESPQNGFHFTRQHIIDVLLDI 704
+E +PY L+ + L+V+ ++F++ F S + G R+ II++LL+I
Sbjct: 696 -NAGDELPYPYLRALLKFDTRETLNVISLAFQEREF--SNELGISH-RKRIINLLLEI 749
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.136 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70,315,267
Number of Sequences: 60738
Number of extensions: 3028328
Number of successful extensions: 9868
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 9849
Number of HSP's gapped (non-prelim): 4
length of query: 1198
length of database: 30,389,216
effective HSP length: 118
effective length of query: 1080
effective length of database: 23,222,132
effective search space: 25079902560
effective search space used: 25079902560
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)