ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4692 good E KOG0172 Amino acid transport and metabolism Lysine-ketoglutarate reductase/saccharopine dehydrogenase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII4692 1664847 1663513 -445
(445 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNR050c [E] KOG0172 Lysine-ketoglutarate reductase/saccharopine ... 755 0.0
SPBC3B8.03 [E] KOG0172 Lysine-ketoglutarate reductase/saccharopi... 531 e-151
Hs13027640 [E] KOG0172 Lysine-ketoglutarate reductase/saccharopi... 340 3e-93
CE18095 [E] KOG0172 Lysine-ketoglutarate reductase/saccharopine ... 317 2e-86
7297296 [E] KOG0172 Lysine-ketoglutarate reductase/saccharopine ... 303 3e-82
At4g33150 [E] KOG0172 Lysine-ketoglutarate reductase/saccharopin... 253 5e-67
>YNR050c [E] KOG0172 Lysine-ketoglutarate reductase/saccharopine
dehydrogenase
Length = 446
Score = 755 bits (1950), Expect = 0.0
Identities = 372/442 (84%), Positives = 401/442 (90%)
Query: 4 VLLLGSGFVAQPVVDVLSATDGISVTVACRTLNKAQALAKASGSNAISLDVSDPKQLDAA 63
VLLLGSGFVAQPV+D L+A D I+VTVACRTL AQALAK SGS AISLDV+D LD
Sbjct: 5 VLLLGSGFVAQPVIDTLAANDDINVTVACRTLANAQALAKPSGSKAISLDVTDDSALDKV 64
Query: 64 LADHDLVISLIPYTYHPAVVQSAIRLKKNVVTSSYISDALRELEPAIKEAGITVMNEIGL 123
LAD+D+VISLIPYT+HP VV+SAIR K +VVTSSYIS ALRELEP I +AGITVMNEIGL
Sbjct: 65 LADNDVVISLIPYTFHPNVVKSAIRTKTDVVTSSYISPALRELEPEIVKAGITVMNEIGL 124
Query: 124 DPGIDHLYAVKTIDEVHKVGGKIKSFVSYCGGLPAPEDSDNPLGYKFSWSSRGVLLALRN 183
DPGIDHLYAVKTIDEVH+ GGK+KSF+SYCGGLPAPEDSDNPLGYKFSWSSRGVLLALRN
Sbjct: 125 DPGIDHLYAVKTIDEVHRAGGKLKSFLSYCGGLPAPEDSDNPLGYKFSWSSRGVLLALRN 184
Query: 184 SAKYWKDGKIETVSSEDLMASAKPYFIYPGYSFVCYPNRDSTLFKELYHIPEADTVIRGT 243
SAKYWKDGKIETVSSEDLMA+AKPYFIYPGY+FVCYPNRDSTLFK+LYHIPEA+TVIRGT
Sbjct: 185 SAKYWKDGKIETVSSEDLMATAKPYFIYPGYAFVCYPNRDSTLFKDLYHIPEAETVIRGT 244
Query: 244 LRYQGFPEFVKALVDIGMLKDEPNELFASPQPWNKALAQYLGAASEKKEDLIKAIDSKTQ 303
LRYQGFPEFVKALVD+GMLKD+ NE+F+ P WN+AL QYLGA S KEDLI +IDSK
Sbjct: 245 LRYQGFPEFVKALVDMGMLKDDANEIFSKPIAWNEALKQYLGAKSTSKEDLIASIDSKAT 304
Query: 304 WKSDDDRERILSGLAWLGLFSDKPFNARGNVLDTLCATLEELMQYEEGERDLVVLQHKFG 363
WK D+DRERILSG AWLGLFSD RGN LDTLCA LEELMQYE+ ERD+VVLQHKFG
Sbjct: 305 WKDDEDRERILSGFAWLGLFSDAKITPRGNALDTLCARLEELMQYEDNERDMVVLQHKFG 364
Query: 364 IEWADGTTETRTSTLVDYGKVGGYSSMAATVGYPVAIATKFVLDGTIKGPGLVAPYSPAI 423
IEWADGTTETRTSTLVDYGKVGGYSSMAATVGYPVAIATKFVLDGTIKGPGL+APYSP I
Sbjct: 365 IEWADGTTETRTSTLVDYGKVGGYSSMAATVGYPVAIATKFVLDGTIKGPGLLAPYSPEI 424
Query: 424 NDPIMKELKEKYGIYLKEKTIA 445
NDPIMKELK+KYGIYLKEKT+A
Sbjct: 425 NDPIMKELKDKYGIYLKEKTVA 446
>SPBC3B8.03 [E] KOG0172 Lysine-ketoglutarate reductase/saccharopine
dehydrogenase
Length = 450
Score = 531 bits (1369), Expect = e-151
Identities = 265/449 (59%), Positives = 341/449 (75%), Gaps = 7/449 (1%)
Query: 1 MSKVLLLGSGFVAQPVVDVLSATDGISVTVACRTLNKAQALAKA-SGSNAISLDVSDPKQ 59
M +LLLGSGFVA P ++ LS ++TVACRTL+KA+A S AI+LDV+D
Sbjct: 1 MPSILLLGSGFVAHPTLEYLSRRKENNITVACRTLSKAEAFINGIPNSKAIALDVNDEAA 60
Query: 60 LDAALADHDLVISLIPYTYHPAVVQSAIRLKKNVVTSSYISDALRELEPAIKEAGITVMN 119
L+ A+++HDL ISLIPYTYH V+++AI+ K+V T+SY++ + ELE A +AG MN
Sbjct: 61 LEKAVSEHDLTISLIPYTYHATVMKAAIKHGKHVCTTSYVNPKMAELEEAAIKAGSICMN 120
Query: 120 EIGLDPGIDHLYAVKTIDEVHKVGGKIKSFVSYCGGLPAPEDSDNPLGYKFSWSSRGVLL 179
EIG+DPGIDHLYA+KTI+EVHK GGKIKSF+SYCGGLPAPEDS+NPLGYKFSWSSRGVLL
Sbjct: 121 EIGVDPGIDHLYAIKTIEEVHKAGGKIKSFLSYCGGLPAPEDSNNPLGYKFSWSSRGVLL 180
Query: 180 ALRNSAKYWKDGKIETVSSEDLMASAKPYFIYPGYSFVCYPNRDSTLFKELYHIPEADTV 239
ALRNSAK++++GK+ + +DLM +AKPYFIYPGY+FVCYPNRDST+++E Y IPEA+T+
Sbjct: 181 ALRNSAKFYENGKLVEIDGKDLMETAKPYFIYPGYAFVCYPNRDSTVYQERYQIPEAETI 240
Query: 240 IRGTLRYQGFPEFVKALVDIGMLKDEPNELFASPQ----PWNKALAQYLGAASEKKEDLI 295
IRGTLRYQGFPEF+ LVD+G L DE + + SP+ PW + A+ + A S + DLI
Sbjct: 241 IRGTLRYQGFPEFIHCLVDMGFL-DETAQEYLSPEAPALPWKEVTARVIKAESSSEADLI 299
Query: 296 KAIDSKTQWKSDDDRERILSGLAWLGLFSDKPFNARGNVLDTLCATLEELMQYEEGERDL 355
K I S ++K +DD++RIL+GL WLG+FS KP RGN LDTLCATLEELMQYEEGERD+
Sbjct: 300 KKISSIHKFKDEDDKKRILNGLKWLGMFSSKPVTPRGNPLDTLCATLEELMQYEEGERDM 359
Query: 356 VVLQHKFGIEWADGTTETRTSTLVDYGKVGGYSSMAATVGYPVAIATKFVLDGTIKGPGL 415
++LQHKF +E +G +TRT TL+DYG GY+SMA VG P +AT+ +LDG I PG+
Sbjct: 360 LILQHKFEVETKEGKRQTRTCTLLDYGVPNGYTSMAKLVGVPCGVATQQILDGVINTPGV 419
Query: 416 VAPYSPAINDPIMKELKEKYGIYLKEKTI 444
+AP + P++ L K GI L+E+ I
Sbjct: 420 LAPNDMKLCGPLIDTL-AKEGIRLEEEII 447
>Hs13027640 [E] KOG0172 Lysine-ketoglutarate reductase/saccharopine
dehydrogenase
Length = 926
Score = 340 bits (871), Expect = 3e-93
Identities = 182/447 (40%), Positives = 271/447 (59%), Gaps = 9/447 (2%)
Query: 3 KVLLLGSGFVAQPVVDVLSATDGISVTVACRTLNKAQALAKASGSNAISLDV-SDPKQLD 61
KVL+LGSG++++PV++ LS I +TV N+ + L K N +S+D+ ++L
Sbjct: 482 KVLVLGSGYISEPVLEYLSRDGNIEITVGSDMKNQIEQLGKKYNINPVSMDICKQEEKLG 541
Query: 62 AALADHDLVISLIPYTYHPAVVQSAIRLKKNVVTSSYISDALRELEPAIKEAGITVMNEI 121
+A DLVISL+PY HP V ++ I K N+VT+SYI+ AL+ELE ++++AGIT++ E+
Sbjct: 542 FLVAKQDLVISLLPYVLHPLVAKACITNKVNMVTASYITPALKELEKSVEDAGITIIGEL 601
Query: 122 GLDPGIDHLYAVKTIDEVHKVGGKIKSFVSYCGGLPAPEDSDNPLGYKFSWSSRGVLLAL 181
GLDPG+DH+ A++TID+ +VG I+S++SYCGGLPAPE S+NPL YKFSWS GVL+ +
Sbjct: 602 GLDPGLDHMLAMETIDKAKEVGATIESYISYCGGLPAPEHSNNPLRYKFSWSPVGVLMNV 661
Query: 182 RNSAKYWKDGKIETVSSE-DLMASAKPYFIYPGYSFVCYPNRDSTLFKELYHIPEADTVI 240
SA Y DGK+ V+ + + +PG + YPNRDST + E+Y I A T++
Sbjct: 662 MQSATYLLDGKVVNVAGGISFLDAVTSMDFFPGLNLEGYPNRDSTKYAEIYGISSAHTLL 721
Query: 241 RGTLRYQGFPEFVKALVDIGMLKDEPNELF---ASPQPWNKALAQYLGAASEKKEDLIKA 297
RGTLRY+G+ + + V +G++ E F A+P W + L +G + + D++K
Sbjct: 722 RGTLRYKGYMKALNGFVKLGLINREALPAFRPEANPLTWKQLLCDLVGISPSSEHDVLKE 781
Query: 298 IDSKTQWKSDDDRERILSGLAWLGLFSDKPFNARGNVLDTLCATLEELMQYEEGERDLVV 357
K K D + L WLGL D+ ++LD L L + Y E+D++V
Sbjct: 782 AVLK---KLGGDNTQ-LEAAEWLGLLGDEQVPQAESILDALSKHLVMKLSYGPEEKDMIV 837
Query: 358 LQHKFGIEWADGTTETRTSTLVDYGKVGGYSSMAATVGYPVAIATKFVLDGTIKGPGLVA 417
++ FGI G E +T LV YG + G+S+MA TVG P A+A K +LDG I GL+
Sbjct: 838 MRDSFGIRHPSGHLEHKTIDLVAYGDINGFSAMAKTVGLPTAMAAKMLLDGEIGAKGLMG 897
Query: 418 PYSPAINDPIMKELKEKYGIYLKEKTI 444
P+S I PI++ +K + IY + TI
Sbjct: 898 PFSKEIYGPILERIKAEGIIYTTQSTI 924
>CE18095 [E] KOG0172 Lysine-ketoglutarate reductase/saccharopine
dehydrogenase
Length = 934
Score = 317 bits (813), Expect = 2e-86
Identities = 174/437 (39%), Positives = 263/437 (59%), Gaps = 5/437 (1%)
Query: 3 KVLLLGSGFVAQPVVDVLSATDGISVTVACRTLNKAQALAKASGSNAISLDVS-DPKQLD 61
+VLLLG+G V+ P D S +++TVA + Q L + ++ +D++ + ++
Sbjct: 492 RVLLLGAGMVSGPFADFYSKQKDVNLTVATESQRDGQRLCTSPNIQSVVVDIARESHTME 551
Query: 62 AALADHDLVISLIPYTYHPAVVQSAIRLKKNVVTSSYISDALRELEPAIKEAGITVMNEI 121
+ +HDLV+SL+P+ +HP V + I ++++VTSSY+S L L+ A K+A +T+MNE
Sbjct: 552 RLIREHDLVVSLLPFNFHPLVAKMCISNQRDMVTSSYVSPELEALDKAAKDADVTIMNEA 611
Query: 122 GLDPGIDHLYAVKTIDEVHKVGGKIKSFVSYCGGLPAPEDSDNPLGYKFSWSSRGVLLAL 181
GLDPGIDH+ A++ D++ + GG+I SF S+CGGLPAPE SDNPL YKFSWS +GVL AL
Sbjct: 612 GLDPGIDHMLAMECFDDIKEHGGRITSFESFCGGLPAPEWSDNPLRYKFSWSPKGVLTAL 671
Query: 182 RNSAKYWKDGKIETVSSEDLMASAKPYFIYPGYSFVCYPNRDSTLFKELYHI-PEADTVI 240
N AKY K+GKI V S ++ + PG + + +PNRDST + ++Y + + T+I
Sbjct: 672 MNPAKYLKNGKIVEVPSGSVVDNLIDIDFMPGLNLIGFPNRDSTKYSDVYGLGNDCKTII 731
Query: 241 RGTLRYQGFPEFVKALVDIGMLKDEPNELFASPQPWNKALAQYLGAASEKKEDLIKAIDS 300
RGTLRYQGF + VKAL +G+L + + F S + + + + S +K D+ DS
Sbjct: 732 RGTLRYQGFVDTVKALHSVGLLSGDNIDSFTSNIGPDLTWKELIASLSNQKLDIFP--DS 789
Query: 301 KTQWKSDDDRERILSGLAWLGLFSDKPFNARGNVLDTLCATLEELMQYEEGERDLVVLQH 360
+ + LS L LGLFSDK + G +DTL L +++ +++ E DLVVL H
Sbjct: 790 LRHIIEEKVGGKGLSALENLGLFSDKVVDRHGTPIDTLAQYLAKILAFKDHESDLVVLNH 849
Query: 361 KFGIEWADGTTETRTSTLVDYGKVGGYSSMAATVGYPVAIATKFVLDGTIKGPGLVAPYS 420
G + G +E +LV YG G+S+MA TVGY AI + VL+ I+ G+ P
Sbjct: 850 DIGAQLPGGNSERHRISLVQYGNPNGFSAMARTVGYTTAIVSHMVLNNEIQRAGIQRPIL 909
Query: 421 PAINDPIMKELKEKYGI 437
+ P +K L++ +GI
Sbjct: 910 KEVYRPALKRLRD-FGI 925
>7297296 [E] KOG0172 Lysine-ketoglutarate reductase/saccharopine
dehydrogenase
Length = 972
Score = 303 bits (776), Expect = 3e-82
Identities = 172/439 (39%), Positives = 259/439 (58%), Gaps = 14/439 (3%)
Query: 3 KVLLLGSGFVAQPVVDVLSATDGISVTVACRTLNKAQALAKA-SGSNAISLDVSDPK-QL 60
KVL+LG+G V+ P+V+ L +S+TV + +A LA+ +G +++ LDV++ L
Sbjct: 529 KVLVLGAGMVSAPLVEWLHREKDVSITVCSQVKEEADRLAQQYAGVDSVYLDVNESTGHL 588
Query: 61 DAALADHDLVISLIPYTYHPAVVQSAIRLKKNVVTSSYISDALRELEPAIKEAGITVMNE 120
D+V+SL+PY+ H V + + ++VT+SY++D + L K G+T+MNE
Sbjct: 589 QELCGRADVVVSLLPYSLHGMVARYCVAEGTHMVTASYLNDEISGLHEEAKAKGVTIMNE 648
Query: 121 IGLDPGIDHLYAVKTIDEVHKVGGKIKSFVSYCGGLPAPEDSDNPLGYKFSWSSRGVLLA 180
+GLDPGIDHL A++ I EV G ++SFVSYCGGLPAPE S+N L YKFSWS RGVLL
Sbjct: 649 VGLDPGIDHLLALECIHEVQDKGAVVESFVSYCGGLPAPEHSNNALRYKFSWSPRGVLLN 708
Query: 181 LRNSAKYWKDGKIETVS-SEDLMASAKPYFIYPGYSFVCYPNRDSTLFKELYHI-PEADT 238
++AKY G+I +S +LM+S + PG++ +PNRDST + LY + + T
Sbjct: 709 TLSAAKYLSQGQIVEISGGGELMSSPRSLDFLPGFALEGFPNRDSTKYGNLYGLGRDVHT 768
Query: 239 VIRGTLRYQGFPEFVKALVDIGMLKDEPNELFASPQP---WNKALAQYLGAASEKKEDLI 295
++RGT+RY+GF E +K + +G++ EP+ L P W + + +G +
Sbjct: 769 LLRGTIRYKGFSESIKPMQLLGLIDPEPHALLHPSGPDVTWRQLVIHLMGMSD------- 821
Query: 296 KAIDSKTQWKSDDDRERILSGLAWLGLFSDKPFNARGNVLDTLCATLEELMQYEEGERDL 355
I + + +R + G+ LGL D P LDTL L + + +E ERDL
Sbjct: 822 STIFYENLKQKLTERIGDVDGIESLGLLDDTPVVKLNTPLDTLSHYLSKRLAFERDERDL 881
Query: 356 VVLQHKFGIEWADGTTETRTSTLVDYGKVGGYSSMAATVGYPVAIATKFVLDGTIKGPGL 415
VVL+H+ GI W DG E R V YG+ G+S+MA TVG P AIA K +LDG I+ G+
Sbjct: 882 VVLRHEVGIRWPDGRREERGINFVVYGQPQGHSAMAMTVGKPAAIAAKMILDGEIQERGV 941
Query: 416 VAPYSPAINDPIMKELKEK 434
+ P++P I P+++ L+ +
Sbjct: 942 LLPFTPDIYRPMLQRLRSE 960
>At4g33150 [E] KOG0172 Lysine-ketoglutarate reductase/saccharopine dehydrogenase
Length = 1064
Score = 253 bits (645), Expect = 5e-67
Identities = 162/477 (33%), Positives = 258/477 (53%), Gaps = 37/477 (7%)
Query: 2 SKVLLLGSGFVAQPVVDVLSATDGIS--------------------VTVACRTLNKAQAL 41
S VL+LG+G V +P D L++ IS V VA L A+
Sbjct: 587 SGVLILGAGRVCRPAADFLASVRTISSQQWYKTYFGADSEEKTDVHVIVASLYLKDAKET 646
Query: 42 AKA-SGSNAISLDVSDPKQLDAALADHDLVISLIPYTYHPAVVQSAIRLKKNVVTSSYIS 100
+ S A+ LDVSD + L ++ D+V+SL+P + H V ++ I LKK++VT+SY+
Sbjct: 647 VEGISDVEAVRLDVSDSESLLKYVSQVDVVLSLLPASCHAVVAKTCIELKKHLVTASYVD 706
Query: 101 DALRELEPAIKEAGITVMNEIGLDPGIDHLYAVKTIDEVHKVGGKIKSFVSYCGGLPAPE 160
D L K AGIT++ E+GLDPGIDH+ A+K I++ H GK+KSF SYCGGLP+P
Sbjct: 707 DETSMLHEKAKSAGITILGEMGLDPGIDHMMAMKMINDAHIKKGKVKSFTSYCGGLPSPA 766
Query: 161 DSDNPLGYKFSWSSRGVLLALRNSAKYWKDGKIETVSSEDLMASAKPYFI--YPGYSFVC 218
++NPL YKFSW+ G + A +N AKY +G I V ++L SA + + P ++ C
Sbjct: 767 AANNPLAYKFSWNPAGAIRAGQNPAKYKSNGDIIHVDGKNLYDSAARFRVPNLPAFALEC 826
Query: 219 YPNRDSTLFKELYHI-PEADTVIRGTLRYQGFPEFVKALVDIGMLKDEPNELFASPQ--P 275
+PNRDS ++ E Y I EA T+ RGTLRY+GF + L +G E N++ ++ +
Sbjct: 827 FPNRDSLVYGEHYGIESEATTIFRGTLRYEGFSMIMATLSKLGFFDSEANQVLSTGKRIT 886
Query: 276 WNKALAQYLGAASEKKEDLIKAID--SKTQWKSDDDRE---RILSGLAWLGLFSDKPF-N 329
+ L+ L ++ + + + + SK K +E + + +LG ++ +
Sbjct: 887 FGALLSNILNKDADNESEPLAGEEEISKRIIKLGHSKETAAKAAKTIVFLGFNEEREVPS 946
Query: 330 ARGNVLDTLCATLEELMQYEEGERDLVVLQHKFGIEWADG-TTETRTSTLVDYGKVGG-- 386
+V D C +EE + Y E+D+V+L H+ +E+ + E T+TL+++G +
Sbjct: 947 LCKSVFDATCYLMEEKLAYSGNEQDMVLLHHEVEVEFLESKRIEKHTATLLEFGDIKNGQ 1006
Query: 387 -YSSMAATVGYPVAIATKFVLDGTIKGPGLVAPYSPAINDPIMKELKEKYGIYLKEK 442
++MA TVG P AI +++ IK G++ P + P + ++ + YGI L EK
Sbjct: 1007 TTTAMAKTVGIPAAIGALLLIEDKIKTRGVLRPLEAEVYLPAL-DILQAYGIKLMEK 1062
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.135 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,067,643
Number of Sequences: 60738
Number of extensions: 1178617
Number of successful extensions: 2946
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2922
Number of HSP's gapped (non-prelim): 6
length of query: 445
length of database: 30,389,216
effective HSP length: 110
effective length of query: 335
effective length of database: 23,708,036
effective search space: 7942192060
effective search space used: 7942192060
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)