ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4751 suspect: %id R KOG0254 General function prediction only Predicted transporter (major facilitator superfamily)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII4751 1680133 1681791 553
(553 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YML116w [R] KOG0254 Predicted transporter (major facilitator sup... 74 8e-13
SPBC1683.03c [R] KOG0254 Predicted transporter (major facilitato... 71 5e-12
SPBC3E7.06c [R] KOG0254 Predicted transporter (major facilitator... 65 3e-10
YBR293w [R] KOG0254 Predicted transporter (major facilitator sup... 57 6e-08
YOR378w [R] KOG0254 Predicted transporter (major facilitator sup... 52 2e-06
>YML116w [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 542
Score = 73.6 bits (179), Expect = 8e-13
Identities = 91/480 (18%), Positives = 184/480 (37%), Gaps = 34/480 (7%)
Query: 25 FICILIACGLDLASVGAMIVLVTDIETRFNVSHTQSGWALTSYVITFAGFIAFFGRVGDI 84
F C +I+ L+ A + ++ + F W + S+ + FI GR+GDI
Sbjct: 73 FTC-MISQLLNQAGTTQTLSIMNILSDSFGSEGNSKSWLMASFPLVSGSFILISGRLGDI 131
Query: 85 VGNGIMMCVFSVIFGIFSLLCAVVP---NFIAFTVFRAFQGMAGAGIVPCAYALVNKMFQ 141
G M+ V V+ I+SL+C + + F + RAFQG+ A ++P ++ ++
Sbjct: 132 YGLKKMLLVGYVLVIIWSLICGITKYSGSDTFFIISRAFQGLGIAFVLPNVLGIIGNIYV 191
Query: 142 GPNLQRYFSILSSVISAMXXXXXXXXXXXXETALGYKXXXXXXXXXXXXXXXVICVLIGY 201
G ++ +I+ S + AM G I I +
Sbjct: 192 GGTFRK--NIVISFVGAMAPIGATLGCLFA----GLIGTEDPKQWPWAFYAYSIAAFINF 245
Query: 202 PELLLNRHSYKEKFQRVYKLDVIGCLLFISGSILLVVGLTQGGES-WNDPTAYVPLAVSI 260
+ S + +D IG +L + G ILL Q S WN V L +S+
Sbjct: 246 VLSIYAIPSTIPTNIHHFSMDWIGSVLGVIGLILLNFVWNQAPISGWNQAYIIVILIISV 305
Query: 261 VLLIIFFMWNLGYAVVLKLIKPISTHKGYAYMESVNLLIPKNLMFSHNFIPVLLATFFLF 320
+ L++F ++ + +A L+P+ ++ + I ++LA FF +
Sbjct: 306 IFLVVFIIYEIRFA--------------------KTPLLPRAVIKDRHMIQIMLALFFGW 345
Query: 321 CGFMVVMYVIANYSATVEGNSMIVASIKIIPMISGLVLANGTVAYKQDLFKPKNALVVGS 380
F + + + + + + A + ++A V + P + + S
Sbjct: 346 GSFGIFTFYYFQFQLNIRQYTALWAGGTYFMFLIWGIIAALLVGFTIKNVSP-SVFLFFS 404
Query: 381 FISILGGAFFVPIKYVDDNLFWKLXXXXXXXXXXXXXXXXCYMITMAIGDAPDQYKALAG 440
++ G+ + V + F I + + P +Y+ +AG
Sbjct: 405 MVAFNVGSIMASVTPVHETYFRTQLGTMIILSFGMDLSFPASSIIFS-DNLPMEYQGMAG 463
Query: 441 GIVQTSGQFGNEVSLSIVISLLGNQTTDKADLRDRFQNASYIVITAATISTLVS-LFLVK 499
+V T + + L + ++ +D L ++ A Y+ I A+++ ++S L++V+
Sbjct: 464 SLVNTVVNYSMSLCLGMGATVETQVNSDGKHLLKGYRGAQYLGIGLASLACMISGLYMVE 523
>SPBC1683.03c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 519
Score = 70.9 bits (172), Expect = 5e-12
Identities = 106/506 (20%), Positives = 186/506 (35%), Gaps = 71/506 (14%)
Query: 13 SLFHQDTLKHLKFICILIACGLDL---ASVGAMIVLVTDIETRFNVSHT-QSGWALTSYV 68
SLFH+ + + IAC L A +G I I ++ Q W SY
Sbjct: 40 SLFHE-------IVFVFIACTAQLMTQAGLGQSIAPNNIIGKSLGTTNPGQLSWFPASYS 92
Query: 69 ITFAGFIAFFGRVGDIVGNGIMMCVFSVIFGIFSLLCA---VVPNFIAFTVFRAFQGMAG 125
+T FI GR+GDI G+ M + + F I+SL+ + I F V RA G+A
Sbjct: 93 LTVGTFILIAGRLGDIYGHKKMFVLGYIWFCIWSLISGFSYYAKSVIMFDVCRALTGIAP 152
Query: 126 AGIVPCAYALVNKMF-QGPNLQRYFS----------ILSSVISAMXXXXXXXXXXXXETA 174
A ++P A AL+ +++ G F+ +L SV S + TA
Sbjct: 153 AFLLPNALALLGRVYPPGKKKNLIFALFGATAPNGFLLGSVFSGIFAQLSWWPWTYWTTA 212
Query: 175 LGYKXXXXXXXXXXXXXXXVICVLIGYPELLLNRHSYKEKFQRVYKLDVIGCLLFISGSI 234
++C++ H ++ + K D +G +SG +
Sbjct: 213 -------------------IVCIVFAIIGYFAIPHIEADEVEEKQKFDYLGAFFGVSGLV 253
Query: 235 LLVVGLTQGG-ESWNDPTAYVPLAVSIVLLIIFFMWNLGYAVVLKLIKPISTHKGYAYME 293
L+ QG W P Y+ L + + L++F + V ++++PI
Sbjct: 254 LINFSWNQGPVVGWQVPYVYILLIIGFLSLVVFVL------VEKRVVQPI---------- 297
Query: 294 SVNLLIPKNLMFSHNFIPVLLATFFLFCGFMVVMYVIANYSATVEGNSMIVASIKIIPM- 352
L P M + VL+ + F + MY + + + + ++ + ++ P+
Sbjct: 298 ----LAPS--MMNSEMGCVLICVAAGWACFGIWMYYLWQFLENLRFATPLLVTAQLTPVG 351
Query: 353 ISGLVLANGTVAYKQDLFKPKNALVVGSFISILGGAFFVPIKYVDDNLFWKLXXXXXXXX 412
ISG A T Y KP +VV S I+ G + + +W
Sbjct: 352 ISGCAAAL-TTGYLLKRLKPTKIMVV-SMIAFTVGTILIATAPIHQT-YWAQTFVSIIVT 408
Query: 413 XXXXXXXXCYMITMAIGDAPDQYKALAGGIVQTSGQFGNEVSLSIVISLLGNQTTDKADL 472
M P Q++ +A +V T + + L I ++ + DL
Sbjct: 409 PWGMDMSFPAATLMLSDFVPKQHQGIAASLVSTVVNYSISIGLGIAGTVETHLNHKGVDL 468
Query: 473 RDRFQNASYIVITAATISTLVSLFLV 498
+++A Y+ + V++ V
Sbjct: 469 IRGYRSAWYMGTGFGGLGVCVAILTV 494
>SPBC3E7.06c [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 577
Score = 65.1 bits (157), Expect = 3e-10
Identities = 95/445 (21%), Positives = 158/445 (35%), Gaps = 49/445 (11%)
Query: 33 GLDLASVGAMIVL--VTDIETRFNVSHTQSGWALTSYVITFAGFIAFFGRVGDIVGNGIM 90
G+ LA++ IV T I F +Q W T+Y+I+ F FG+ DI G
Sbjct: 98 GVVLAALDNTIVASTYTKIGAEFG-KFSQVSWTATAYMISCTAFQPLFGKFCDIYGRKKT 156
Query: 91 MCVFSVIFGIFSLLCAVVPNFIAFTVFRAFQGMAGAGIVPCAYALVNKMF---QGPNLQR 147
+ +FGI LC + RA G+ G G+ L++ + Q Q
Sbjct: 157 LLAAYCVFGIGCFLCGTSRSLWQLVAARAIAGIGGGGMNSTVSILMSDIVPLKQRGTYQG 216
Query: 148 YFSILSSVISAMXXXXXXXXXXXXETALGYKXXXXXXXXXXXXXXXVICVLIGYPELLLN 207
++ ++ S++ +G+ I L Y L L
Sbjct: 217 IINVFFAIGSSLGGPVGGYFADQYTWRIGF---------LIQVPLIAIAFLCVYFTLNLP 267
Query: 208 RHSYKEKFQRVYKLDVIGCLLFISGSILLVVGLTQGG--ESWNDPTAYVPLAVSIVLLII 265
H++ R K+D+ G +L I G + T GG WNDP L S + +
Sbjct: 268 HHNHVSFMTRFRKIDLKGLILLIIGVTTMTCAFTLGGNVREWNDPVVISLLIASSISYLS 327
Query: 266 FFMWNLGYAVVLKLIKPISTHKGYAYMESVNLLIPKNLMFSHNFIPVLLATFFLFCGFMV 325
F Y +P L P +++ + L FF
Sbjct: 328 FV-----YVEAFVAFEP---------------LAPMDVLTERTCLSSYLCNFFHSVANFG 367
Query: 326 VMYVIANYSATVEGNSMIVASIKIIPMISGLVLANGTVAYKQDLFKPKNALVVGSFISIL 385
+Y + + +++ + I++IPMI G L + L + VGS+
Sbjct: 368 WIYGMPLFFQSIKNEGAEKSGIRLIPMIIGSSLGSLLGGAVISLTGNYKKITVGSYFFGS 427
Query: 386 GGAFFVPIKYVDDNLFWKLXXXXXXXXXXXXXXXXCYMITMAIGDAPDQYKALAGGIVQT 445
A F+ ++Y N W+ ++ + I +P ++A A + T
Sbjct: 428 VAALFM-LRYGYSNFNWEYAVYPFSGGLGNGIAVTTTLVAI-IHASPSAFQASA---IAT 482
Query: 446 SGQF-------GNEVSLSIVISLLG 463
S F G +S SIV ++LG
Sbjct: 483 SYLFRSNGCVLGVSISSSIVQTVLG 507
>YBR293w [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 474
Score = 57.4 bits (137), Expect = 6e-08
Identities = 53/265 (20%), Positives = 104/265 (39%), Gaps = 34/265 (12%)
Query: 60 SGWALTSYVITFAGFIAFFGRVGDIVGNGIMMCVFSVIFGIFSLLCAVVPNFIAFTVFRA 119
S W T+Y+IT F +G D +G + + F I L C N + RA
Sbjct: 4 SNWITTAYLITSTSFQPLYGSFSDALGRRNCLFFANGAFTIGCLACGFSKNIYMLSFMRA 63
Query: 120 FQGMAGAGIVPCAYALVNKMFQGPNLQRYFSILSSVISAMXXXXXXXXXXXXETALGYKX 179
G+ G G++ + +VN + + F +++ +++G++
Sbjct: 64 LTGIGGGGLITLS-TIVNSDVIPSSKRGIFQAFQNLLLGFGAICGASFGGTIASSIGWR- 121
Query: 180 XXXXXXXXXXXXXXVICVLIGYPELLLNRHSYKEKFQRVYK--------LDVIGCLLFIS 231
+ +L+ Y + N+ Y + +++ +DV+G +L I+
Sbjct: 122 ---WCFLIQVPISVISSILMNY--YVPNQKEYNRQNSSIFQNPGKILRDIDVMGSILIIT 176
Query: 232 GSIL----LVVGLTQGGESWNDPTAYVPLAVSIVLLIIFFMW---------------NLG 272
G L L +G + SW P+ + L S+++L++F + N
Sbjct: 177 GLTLQLLYLSLGCSTSKLSWTSPSVLLLLVGSVIILLLFILHERKTSARAIIPMELVNSS 236
Query: 273 YAVVLKLIKPISTHKGYAYMESVNL 297
Y+VV+ I + YAY+ ++ L
Sbjct: 237 YSVVVLSISILVGFASYAYLFTLPL 261
>YOR378w [R] KOG0254 Predicted transporter (major facilitator superfamily)
Length = 515
Score = 52.0 bits (123), Expect = 2e-06
Identities = 55/240 (22%), Positives = 92/240 (37%), Gaps = 19/240 (7%)
Query: 37 ASVGAMIVLVTDIETRFNVSHT-QSGWALTSYVITFAGFIAFFGRVGDIVGNGIMMCVFS 95
A +G + + I F ++ Q W ++Y +T FI GR+GDI G+ +
Sbjct: 58 AGLGQSLAPLHIIGNSFGTTNAGQLSWFASAYSLTVGTFILIAGRLGDIFGHKKFFVLGF 117
Query: 96 VIFGIFSLLC--AVVPNFIAFTVFRAFQGMAGAGIVPCAYALVNKMFQ-GPNLQRYFSIL 152
+ ++SLL +V N I F RAFQGM A ++P A A++ + ++ G FS+
Sbjct: 118 FWYALWSLLAGFSVYSNQIFFDCCRAFQGMGPAFLLPNAIAILGRTYKPGRRKNMVFSLF 177
Query: 153 SSVISAMXXXXXXXXXXXXETALGYKXXXXXXXXXXXXXXXVICVLIGYPELLLNRHS-- 210
+ + A + C ++ + H+
Sbjct: 178 GASAPGGFFLGAVFSSMLGQLA---------WWPWAYWIMGIACFVLAVAGYFVIPHTPM 228
Query: 211 ---YKEKFQRVYKLDVIGCLLFISGSILLVVGLTQGG-ESWNDPTAYVPLAVSIVLLIIF 266
F+ + ++D G + + G IL QG W P Y L V L+IF
Sbjct: 229 PSRDASSFKLLERIDFAGSVTGVVGLILFNFAWNQGPVVGWQTPYTYALLIVGTFFLVIF 288
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.327 0.142 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,430,159
Number of Sequences: 60738
Number of extensions: 1017512
Number of successful extensions: 2930
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 2919
Number of HSP's gapped (non-prelim): 9
length of query: 553
length of database: 30,389,216
effective HSP length: 111
effective length of query: 442
effective length of database: 23,647,298
effective search space: 10452105716
effective search space used: 10452105716
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)