ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4883 good F KOG1436 Nucleotide transport and metabolism Dihydroorotate dehydrogenase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII4883 1732223 1731279 -315
(315 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL216w [F] KOG1436 Dihydroorotate dehydrogenase 460 e-129
SPAC57A10.12c [F] KOG1436 Dihydroorotate dehydrogenase 65 1e-10
At3g17810 [F] KOG1799 Dihydropyrimidine dehydrogenase 61 2e-09
CE14420 [F] KOG1436 Dihydroorotate dehydrogenase 58 2e-08
7299059 [F] KOG1436 Dihydroorotate dehydrogenase 56 6e-08
Hs16753223 [F] KOG1436 Dihydroorotate dehydrogenase 52 9e-07
Hs4503373 [F] KOG1799 Dihydropyrimidine dehydrogenase 50 4e-06
>YKL216w [F] KOG1436 Dihydroorotate dehydrogenase
Length = 314
Score = 460 bits (1184), Expect = e-129
Identities = 225/314 (71%), Positives = 254/314 (80%)
Query: 1 MKPSLQVEFCGHIYENPLMNASGVCCMTTNELDDMARSSSGAFITKSATSMERDGNPEPR 60
M SL +F + YENP MNASGV CMTT ELD++A S +GAFITKSAT++ER+GNPEPR
Sbjct: 1 MTASLTTKFLNNTYENPFMNASGVHCMTTQELDELANSKAGAFITKSATTLEREGNPEPR 60
Query: 61 YVSVPLGSINSMGLPNKGIDYYLDYVLDRQDRFPSENPPFMSVAGMSIDENIKLLHRIQD 120
Y+SVPLGSINSMGLPN+GIDYYL YVL+RQ +P F SVAGMSIDEN+ LL +IQD
Sbjct: 61 YISVPLGSINSMGLPNEGIDYYLSYVLNRQKNYPDAPAIFFSVAGMSIDENLNLLRKIQD 120
Query: 121 SNFRGITELNLSCPNVPGKPQVAYDFELTDQILSSVFEFFTKPLGVKLPPYFDFAHFDAM 180
S F GITELNLSCPNVPGKPQVAYDF+LT + L VF FF KPLGVKLPPYFDFAHFD M
Sbjct: 121 SEFNGITELNLSCPNVPGKPQVAYDFDLTKETLEKVFAFFKKPLGVKLPPYFDFAHFDIM 180
Query: 181 ACILNKYPIAYVNCINSVGNGLYIDVEKESVVIKPKDGFGGIGGEYIKPTALANVRAFYN 240
A ILN++P+AYVN INS+GNGL+IDVEKESVV+KPK+GFGGIGGEY+KPTALANVRAFY
Sbjct: 181 AKILNEFPLAYVNSINSIGNGLFIDVEKESVVVKPKNGFGGIGGEYVKPTALANVRAFYT 240
Query: 241 RLNXXXXXXXXXXXXXXQDAFEHLLCGATMLQIGTELQNEGTEIFERINAELLQILEQKG 300
RL +DAFEHLLCGA+MLQIGTELQ EG +IFERI EL I+E KG
Sbjct: 241 RLRPEIKVIGTGGIKSGKDAFEHLLCGASMLQIGTELQKEGVKIFERIEKELKDIMEAKG 300
Query: 301 YQSIEDFRGKLRSI 314
Y SI+ FRGKL SI
Sbjct: 301 YTSIDQFRGKLNSI 314
>SPAC57A10.12c [F] KOG1436 Dihydroorotate dehydrogenase
Length = 443
Score = 65.1 bits (157), Expect = 1e-10
Identities = 84/343 (24%), Positives = 132/343 (37%), Gaps = 48/343 (13%)
Query: 3 PSLQVEFCGHIYENPLMNASGVCCMTTNELDDMARSSSGAFITKSATSMERDGNPEPRYV 62
PSL VE G + NP+ A+G + + + S T + GNP+PRY
Sbjct: 103 PSLAVEVWGKKFCNPIGLAAGFD-KQADAISGLLNFGFSYLEIGSVTPKPQPGNPKPRYF 161
Query: 63 SVP--LGSINSMGLPNKGIDYYLDYVLDRQDRFPSENPPFM------------------- 101
+ L IN G + G D L + R ++ ++ P +
Sbjct: 162 RLKPDLSVINRYGFNSIGHDAILAKIQKRVRKYIAKTSPQLLKQFDANPASCTDPAVLGV 221
Query: 102 -------SVAGMSIDENIKLLHRIQD--------SNFRGITELNLSCPNVPGKPQVAYDF 146
G+++ +N K + I+D NF I +N+S PN PG +
Sbjct: 222 PRSLIPNKFLGINLGKN-KNGNEIEDYVEGVRTFGNFADILVINVSSPNTPGLRNLQKKS 280
Query: 147 ELTDQILSSVFEFFT-----KPLGVKLPPYFDFAHFDAMACILNKYPIAYVNCINSVGNG 201
L+ + + V E P+ VK+ P + +A +L K I V VGN
Sbjct: 281 ALSTLLTAVVSERNKLNSPHPPVLVKIAPDLNEEELTDIADVLKKCKIDGV----IVGNT 336
Query: 202 LYIDVEKESVVIKPKDGFGGIGGEYIKPTALANVRAFYNRLNXXXXXXXXXXXXXXQDAF 261
+ K + GG+ G +KP AL +R L+ +DA
Sbjct: 337 T-VQRPKTLKSTSHVEETGGLSGPPLKPIALNTLRTLRKHLSSDIPIIGCGGISSGKDAI 395
Query: 262 EHLLCGATMLQIGTELQNEGTEIFERINAELLQILEQKGYQSI 304
E+ GATM+Q+ T L +G I +I E+L L+ K + I
Sbjct: 396 EYARAGATMVQVYTALGYDGPVIAHKIKQEILAELKGKRWVDI 438
>At3g17810 [F] KOG1799 Dihydropyrimidine dehydrogenase
Length = 426
Score = 60.8 bits (146), Expect = 2e-09
Identities = 78/336 (23%), Positives = 140/336 (41%), Gaps = 37/336 (11%)
Query: 2 KPSLQVEFCGHIYENPLMNASGVCCMTTNELDDMARSSSGAFITKSAT-SMERDGNPEPR 60
+P L V G NP + SG + GA I K+ + + N PR
Sbjct: 49 EPDLSVTVNGLKMPNPFVIGSGPPGTNYTVMKRAFDEGWGAVIAKTVSLDASKVINVTPR 108
Query: 61 YVSVPLGSINS-----MGLPNKGI--DYYLDYVLDRQDRFPSENPPFMSVAGMSIDENI- 112
Y + GS S +G N + D L+ +L +R E P + +A + + N
Sbjct: 109 YARLRTGSNGSAKTDVIGWQNIELISDRPLETMLKEFERLKKEYPDRILIASVMEEYNKT 168
Query: 113 ---KLLHRIQDSNFRGITELNLSCPNVPGKPQ------VAYDFELTDQILSSVFEFFTKP 163
+L+ R++ + + E+N SCP+ G P+ V D L D++ + T P
Sbjct: 169 AWEELIDRVEQTGVDAL-EINFSCPH--GMPERRMGAAVGQDCALLDEVCGWINAKATVP 225
Query: 164 LGVKLPPYFDFAHFDAMACILNKYPIAYVNCINSVGNGLYIDVEKESVVIKPK---DGF- 219
+ K+ P + A + K + IN++ + + ID++ ++P+ +G+
Sbjct: 226 VWAKMTP--NITDITEPARVSLKSGCEGIAAINTIMSVMGIDMK----TLRPEPCVEGYS 279
Query: 220 --GGIGGEYIKPTALANV----RAFYNRLNXXXXXXXXXXXXXXQDAFEHLLCGATMLQI 273
GG + ++P ALA V + + + DA E +L G+ +Q+
Sbjct: 280 TPGGYSYKAVRPIALAKVMNIAKMMKSEFSEDRSLSGIGGVETGYDAAEFILLGSNTVQV 339
Query: 274 GTELQNEGTEIFERINAELLQILEQKGYQSIEDFRG 309
T + G + + AEL ++Q + +IE+FRG
Sbjct: 340 CTGVMMHGYGHVKTLCAELKDFMKQHNFSTIEEFRG 375
>CE14420 [F] KOG1436 Dihydroorotate dehydrogenase
Length = 411
Score = 58.2 bits (139), Expect = 2e-08
Identities = 71/322 (22%), Positives = 131/322 (40%), Gaps = 21/322 (6%)
Query: 3 PSLQVEFCGHIYENPLMNASGVCCMTTNELDDMARSSS-GAFITKSATSMERDGNPEPRY 61
P L+ E G ++NP+ A+G + +A++S G S T + + GN PR
Sbjct: 88 PELKCELFGREFKNPIGLAAGFD-KDGQAITQLAKNSGFGLIEIGSVTPIPQPGNNRPRV 146
Query: 62 VSV--PLGSINSMGLPNKGIDYYLDYVLDRQDRFPSENPPFMSV--AGMSIDENIKLLHR 117
+ G IN G + G+ V +D + E+ + V + E+ KL +
Sbjct: 147 FRLLEDEGVINRYGFNSDGVGRVHQRVRSARDSWVPESYAYFGVNLGKNKLTEDAKLDYE 206
Query: 118 IQDSNFRGITE---LNLSCPNVPGKPQVAYDFELTDQILSSVFEFFTKPLGVKLPPYFDF 174
I + F + LN+S PN PG + +L +++L+ V + K P F
Sbjct: 207 IGVNYFAPHCDYLVLNVSSPNTPGLRSMQKKSDL-EKLLAYVHQALEMHKLEKQPQVFLK 265
Query: 175 AHFDAMACILNKYPIAYVNCINSVGNGLYIDVEKESVVIKP-------KDGFGGIGGEYI 227
D + L N ++ +G+ + + + +P K GG+ G +
Sbjct: 266 IAPDLIESELKDIAQVVTNKKFAI-DGIIVS---NTTIARPDYLRSENKTETGGLSGAPV 321
Query: 228 KPTALANVRAFYNRLNXXXXXXXXXXXXXXQDAFEHLLCGATMLQIGTELQNEGTEIFER 287
+ + VR Y N DA+E + GA+++Q+ + EG + +
Sbjct: 322 REISTECVRKIYKLTNGQIPIIGCGGVFSGADAYEKIRAGASLVQLYSAFVYEGFPVIGK 381
Query: 288 INAELLQILEQKGYQSIEDFRG 309
I EL+++L + G+ + + G
Sbjct: 382 IKRELVELLRKDGFSHVSEAIG 403
>7299059 [F] KOG1436 Dihydroorotate dehydrogenase
Length = 405
Score = 56.2 bits (134), Expect = 6e-08
Identities = 69/322 (21%), Positives = 123/322 (37%), Gaps = 26/322 (8%)
Query: 4 SLQVEFCGHIYENPLMNASGVCCMTTNELDDMARSSSGAFITKSATSMERDGNPEPRY-- 61
+L F G + NP+ A+G +D + G + T ++GNP+PR
Sbjct: 85 NLHTSFFGRMLSNPIGIAAGFD-KNAEAVDGLQDLGFGFIEVGTVTPAAQEGNPKPRVFR 143
Query: 62 VSVPLGSINSMGLPNKGIDYYLDYVLDRQDRFPSENPPFMSVAGMSIDENIKLLHRIQD- 120
++ IN G + G L + R + F V G+++ N + I D
Sbjct: 144 LTEDKAIINRYGFNSDGHQAVLQRL-----RLLRKKENFNGVVGVNLGRNKTTMSPIADY 198
Query: 121 ----SNFRGITE---LNLSCPNVPG------KPQVAYDFELTDQILSSVFEFFTKPLGVK 167
F + + +N+S PN G K ++ E + SS+ + P+ +K
Sbjct: 199 VQGVRVFGPVADYLVINVSSPNTKGLRDMQSKEKLRELLEQVNDTKSSLDKNKNVPILLK 258
Query: 168 LPPYFDFAHFDAMACILNKYPIAYVNCINSVGNGLYIDVEKESVVIKPKDGFGGIGGEYI 227
L P + ++ + I S ++EK + + GG+ G +
Sbjct: 259 LSPDLSLDDMKDIVWVIKRKKSRVDGLIVSNTTVSRENIEKNKLA----EETGGLSGPPL 314
Query: 228 KPTALANVRAFYNRLNXXXXXXXXXXXXXXQDAFEHLLCGATMLQIGTELQNEGTEIFER 287
K + + Y + DA+E + GA+ +QI T L EG + E
Sbjct: 315 KARSTEMIAQMYQLTDGKIPIIGVGGVASGYDAYEKIEAGASYVQIYTALVYEGPALVED 374
Query: 288 INAELLQILEQKGYQSIEDFRG 309
I AEL ++ + G+ ++ D G
Sbjct: 375 IKAELSALITRLGHTNVADVVG 396
>Hs16753223 [F] KOG1436 Dihydroorotate dehydrogenase
Length = 395
Score = 52.4 bits (124), Expect = 9e-07
Identities = 70/329 (21%), Positives = 131/329 (39%), Gaps = 40/329 (12%)
Query: 5 LQVEFCGHIYENPLMNASGVCCMTTNELDDMARSSSGAFITKSATSMERDGNPEPRYVSV 64
L+V GH + NP+ A+G +D + + G S T ++GNP PR +
Sbjct: 78 LEVRVLGHKFRNPVGIAAGFD-KHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRL 136
Query: 65 PLGS--INSMGLPNKG---IDYYLDYVLDRQDRFPSENPPFMSVAGM---SID------E 110
P IN G + G +++ L +Q + + P G S+D E
Sbjct: 137 PEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNKTSVDAAEDYAE 196
Query: 111 NIKLLHRIQDSNFRGITELNLSCPNVPGKPQVAYDFELTDQILSSVFE-------FFTKP 163
+++L + D +N+S PN G + EL ++L+ V +
Sbjct: 197 GVRVLGPLAD-----YLVVNVSSPNTAGLRSLQGKAELR-RLLTKVLQERDGLRRVHRPA 250
Query: 164 LGVKLPPYFDFAHFDAMACILNKYPIAYVNCINSVGN---GLYIDVEKESVVIKPKDGFG 220
+ VK+ P + +A ++ + I + N+ + GL + E+ G
Sbjct: 251 VLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQGALRSET---------G 301
Query: 221 GIGGEYIKPTALANVRAFYNRLNXXXXXXXXXXXXXXQDAFEHLLCGATMLQIGTELQNE 280
G+ G+ ++ + +R Y QDA E + GA+++Q+ T L
Sbjct: 302 GLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFW 361
Query: 281 GTEIFERINAELLQILEQKGYQSIEDFRG 309
G + ++ EL +L+++G+ + D G
Sbjct: 362 GPPVVGKVKRELEALLKEQGFGGVTDAIG 390
>Hs4503373 [F] KOG1799 Dihydropyrimidine dehydrogenase
Length = 1025
Score = 50.1 bits (118), Expect = 4e-06
Identities = 75/325 (23%), Positives = 131/325 (40%), Gaps = 24/325 (7%)
Query: 5 LQVEFCGHIYENPLMNASGVCCMTTNELDDMARSSSGAFITKSATSMERD--GNPEPRYV 62
+ VE G + NP AS +T+ + + G +TK+ S+++D N PR +
Sbjct: 533 ISVEMAGLKFINPFGLASATPATSTSMIRRAFEAGWGFALTKTF-SLDKDIVTNVSPRII 591
Query: 63 ----SVPL------GSINSMGLPNKGIDYYLDYVLDRQDRFPSENPPFMSVAGMSIDENI 112
S P+ +N + K Y+ V + + FP + + ++
Sbjct: 592 RGTTSGPMYGPGQSSFLNIELISEKTAAYWCQSVTELKADFPDNIVIASIMCSYNKNDWT 651
Query: 113 KLLHRIQDSNFRGITELNLSCPNVPGKPQVAY----DFELTDQILSSVFEFFTKPLGVKL 168
+L + +DS + ELNLSCP+ G+ + D EL I V + P KL
Sbjct: 652 ELAKKSEDSGADAL-ELNLSCPHGMGERGMGLACGQDPELVRNICRWVRQAVQIPFFAKL 710
Query: 169 PPYFDFAHFDAMACILNKYPIAYVNCINSVGN--GLYID-VEKESVVIKPKDGFGGIGGE 225
P + ++A + V N+V GL D +V I + +GG+ G
Sbjct: 711 TP--NVTDIVSIARAAKEGGANGVTATNTVSGLMGLKSDGTPWPAVGIAKRTTYGGVSGT 768
Query: 226 YIKPTALANVRAFYNRLNXXXXXXXXXXXXXXQDAFEHLLCGATMLQIGTELQNEGTEIF 285
I+P AL V + L + + L GA++LQ+ + +QN+ +
Sbjct: 769 AIRPIALRAVTSIARALPGFPILATGGIDSA-ESGLQFLHSGASVLQVCSAIQNQDFTVI 827
Query: 286 ERINAELLQILEQKGYQSIEDFRGK 310
E L +L K + ++D+ G+
Sbjct: 828 EDYCTGLKALLYLKSIEELQDWDGQ 852
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.139 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,911,649
Number of Sequences: 60738
Number of extensions: 815411
Number of successful extensions: 1760
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1751
Number of HSP's gapped (non-prelim): 8
length of query: 315
length of database: 30,389,216
effective HSP length: 106
effective length of query: 209
effective length of database: 23,950,988
effective search space: 5005756492
effective search space used: 5005756492
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)