ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII4891 good C KOG3857 Energy production and conversion Alcohol dehydrogenase, class IV
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII4891 1734340 1733087 -418
(418 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL256w [C] KOG3857 Alcohol dehydrogenase class IV 548 e-156
SPAC5H10.06c [C] KOG3857 Alcohol dehydrogenase class IV 460 e-129
Hs21389519 [C] KOG3857 Alcohol dehydrogenase class IV 117 4e-26
CE24222 [C] KOG3857 Alcohol dehydrogenase class IV 114 2e-25
7291405 [C] KOG3857 Alcohol dehydrogenase class IV 110 3e-24
>YGL256w [C] KOG3857 Alcohol dehydrogenase class IV
Length = 465
Score = 548 bits (1411), Expect = e-156
Identities = 261/384 (67%), Positives = 320/384 (82%)
Query: 35 KEMSSVVGFYMPTVSYFGEGALDEVADYIKSQNFKRPVIITDPGISKIGLTDRVASLLEK 94
++MSSV GFY+P +S+FGEGAL+E ADYIK++++K+ +I+TDPGI+ IGL+ RV +LE+
Sbjct: 82 RKMSSVTGFYIPPISFFGEGALEETADYIKNKDYKKALIVTDPGIAAIGLSGRVQKMLEE 141
Query: 95 RGSAVGIYDQTQPNPTTSNVNAGLVVLKEHNXXXXXXXXXXXAHDNAKAIALLATNGGEI 154
R V IYD+TQPNP +NV AGL VLKE N AHDNAKAIALLATNGGEI
Sbjct: 142 RDLNVAIYDKTQPNPNIANVTAGLKVLKEQNSEIVVSIGGGSAHDNAKAIALLATNGGEI 201
Query: 155 GDYEGVNKSKNKALPMVAVNTTAGTASEMTKFTIITNEEKKVKMAIIDNHITPSVAVNDP 214
GDYEGVN+SK ALP+ A+NTTAGTASEMT+FTII+NEEKK+KMAIIDN++TP+VAVNDP
Sbjct: 202 GDYEGVNQSKKAALPLFAINTTAGTASEMTRFTIISNEEKKIKMAIIDNNVTPAVAVNDP 261
Query: 215 SIMYGLPPSLTAATGLDALTHCVESYVSTAANPITDTCALKGVELIQEHLLNAFENGKDT 274
S M+GLPP+LTAATGLDALTHC+E+YVSTA+NPITD CALKG++LI E L+ A+++GKD
Sbjct: 262 STMFGLPPALTAATGLDALTHCIEAYVSTASNPITDACALKGIDLINESLVAAYKDGKDK 321
Query: 275 TARTGMCYAQYLAGMAFNNASLGYVHSIAHQLGGFYHLPHGVCNAVLLPHVQSFNKKDPR 334
ART MCYA+YLAGMAFNNASLGYVH++AHQLGGFYHLPHGVCNAVLLPHVQ N + P+
Sbjct: 322 KARTDMCYAEYLAGMAFNNASLGYVHALAHQLGGFYHLPHGVCNAVLLPHVQEANMQCPK 381
Query: 335 ANERLSQIAPHLGAKDATADALVERLLEFTKSLGIPRNLKELGVKEEDFEILAEHALKDV 394
A +RL +IA H GA + ++ L +++ IPRNLKELGVK EDFEILAEHA+ D
Sbjct: 382 AKKRLGEIALHFGASQEDPEETIKALHVLNRTMNIPRNLKELGVKTEDFEILAEHAMHDA 441
Query: 395 CGLTNPIQFTKDEVIAIIRQAYEY 418
C LTNP+QFTK++V+AII++AYEY
Sbjct: 442 CHLTNPVQFTKEQVVAIIKKAYEY 465
>SPAC5H10.06c [C] KOG3857 Alcohol dehydrogenase class IV
Length = 422
Score = 460 bits (1183), Expect = e-129
Identities = 224/376 (59%), Positives = 279/376 (73%)
Query: 40 VVGFYMPTVSYFGEGALDEVADYIKSQNFKRPVIITDPGISKIGLTDRVASLLEKRGSAV 99
V FY+P+ + FG+G L E A IK FK +I+TDPGI K+GL D+V +LLE++ V
Sbjct: 46 VSAFYIPSFNLFGKGCLAEAAKQIKMSGFKNTLIVTDPGIIKVGLYDKVKALLEEQSITV 105
Query: 100 GIYDQTQPNPTTSNVNAGLVVLKEHNXXXXXXXXXXXAHDNAKAIALLATNGGEIGDYEG 159
+YD QPNPT NVN GL ++K+ N AHD AK IALLATNGG+I DYEG
Sbjct: 106 HLYDGVQPNPTVGNVNQGLEIVKKENCDSMVSIGGGSAHDCAKGIALLATNGGKIADYEG 165
Query: 160 VNKSKNKALPMVAVNTTAGTASEMTKFTIITNEEKKVKMAIIDNHITPSVAVNDPSIMYG 219
V+KS LP++A+NTTAGTASEMT+F IIT E + +KMAIID H P ++VNDP MYG
Sbjct: 166 VDKSSKPQLPLIAINTTAGTASEMTRFAIITEETRHIKMAIIDKHTMPILSVNDPETMYG 225
Query: 220 LPPSLTAATGLDALTHCVESYVSTAANPITDTCALKGVELIQEHLLNAFENGKDTTARTG 279
LPPSLTAATG+DALTH VE+YVSTAANPITD CA+K +EL+ ++L A +NGKD AR
Sbjct: 226 LPPSLTAATGMDALTHAVEAYVSTAANPITDACAVKCIELVNKYLKRAVDNGKDEEARDN 285
Query: 280 MCYAQYLAGMAFNNASLGYVHSIAHQLGGFYHLPHGVCNAVLLPHVQSFNKKDPRANERL 339
M YA++L GMAFNNASLGYVH++AHQLGGFY +PHGVCNAVLL HVQ FN +DPRAN RL
Sbjct: 286 MAYAEFLGGMAFNNASLGYVHAMAHQLGGFYGIPHGVCNAVLLAHVQKFNSRDPRANARL 345
Query: 340 SQIAPHLGAKDATADALVERLLEFTKSLGIPRNLKELGVKEEDFEILAEHALKDVCGLTN 399
IA HLG ++ TA+A ++R+ + + I +L +LGVKE+DF++L +HA+KD CG TN
Sbjct: 346 GDIAFHLGCEEHTAEAALDRISQLVLEVKIRPHLVDLGVKEKDFDVLVDHAMKDACGATN 405
Query: 400 PIQFTKDEVIAIIRQA 415
PIQ T DEV AI + A
Sbjct: 406 PIQPTHDEVKAIFKSA 421
>Hs21389519 [C] KOG3857 Alcohol dehydrogenase class IV
Length = 419
Score = 117 bits (292), Expect = 4e-26
Identities = 100/385 (25%), Positives = 157/385 (39%), Gaps = 46/385 (11%)
Query: 51 FGEGALDEVADYIKSQNFKRPVIITDPGISKIGLTDRVASLLEKRGSAVGIYDQTQPNPT 110
+G EV +K+ K ++TD +SK+ L K G +YD + PT
Sbjct: 8 YGAAVTKEVGMDLKNMGAKNVCLMTDKNLSKLPPVQVAMDSLVKNGIPFTVYDNVRVEPT 67
Query: 111 TSNVNAGLVVLKEHNXXXXXXXXXXXAHDNAKAIALLATNG-GEIGDYEGVNKSKNKAL- 168
S+ + ++ D KA L A++ + DY K K +
Sbjct: 68 DSSFMEAIEFAQKGAFDAYVAVGGGSTMDTCKAANLYASSPHSDFLDYVSAPIGKGKPVS 127
Query: 169 ----PMVAVNTTAGTASEMTKFTIITNEEKKVKMAIIDNHITPSVAVNDPSIMYGLPPSL 224
P++AV TT+GT SE T I E KVK+ I I P++ + DP +P +
Sbjct: 128 VPLKPLIAVPTTSGTGSETTGVAIFDYEHLKVKIGITSRAIKPTLGLIDPLHTLHMPARV 187
Query: 225 TAATGLDALTHCVESYVS-------------------TAANPITDTCALKGVELIQEHLL 265
A +G D L H +ESY + +NPI+D A+ + ++ ++L
Sbjct: 188 VANSGFDVLCHALESYTTLPYHLRSPCPSNPITRPAYQGSNPISDIWAIHALRIVAKYLK 247
Query: 266 NAFENGKDTTARTGMCYAQYLAGMAFNNASLGYVHSIAHQLGGFYHL------------- 312
A N D AR+ M A AG+ F NA + H +++ + G +
Sbjct: 248 RAVRNPDDLEARSHMHLASAFAGIGFGNAGVHLCHGMSYPISGLVKMYKAKDYNVDHPLV 307
Query: 313 PHGVCNAVLLPHVQSFNKKDPRANERLSQIAPHLGAKDATA---DA---LVERLLEFTKS 366
PHG+ + P V +F + ER ++A LGA TA DA L + L +F
Sbjct: 308 PHGLSVVLTSPAVFTFTAQ--MFPERHLEMAEILGADTRTARIQDAGLVLADTLRKFLFD 365
Query: 367 LGIPRNLKELGVKEEDFEILAEHAL 391
L + L +G + D L + L
Sbjct: 366 LDVDDGLAAVGYSKADIPALVKGTL 390
>CE24222 [C] KOG3857 Alcohol dehydrogenase class IV
Length = 465
Score = 114 bits (286), Expect = 2e-25
Identities = 98/407 (24%), Positives = 166/407 (40%), Gaps = 47/407 (11%)
Query: 51 FGEGALDEVADYIKSQNFKRPVIITDPGISKIGLTDRVASLLEKRGSAVGIYDQTQPNPT 110
FG+G E+ +++ K+ ++ITD + L+ ++D PT
Sbjct: 53 FGKGVTLEIGYDVRNLGAKKTLLITDKNVQNTIAFKNAEQALKMVNIEYEVFDDVLIEPT 112
Query: 111 TSNVNAGLVVLKEHNXXXXXXXXXXXAHDNAKAIALLATNG-GEIGDYEG--VNKS---K 164
+++ + K D KA AL A+N + D+ G KS K
Sbjct: 113 VNSMQKAIAFAKSKQFDSFIAVGGGSVIDTTKAAALYASNPEADFLDFVGPPFGKSMQPK 172
Query: 165 NKALPMVAVNTTAGTASEMTKFTIITNEEKKVKMAIIDNHITPSVAVNDPSIMYGLPPSL 224
N LP++AV TTAGT SE T I+ E K K I I P +AV DP + +P ++
Sbjct: 173 NPMLPLIAVPTTAGTGSETTAAAIMDLPEHKCKTGIRLRCIKPYLAVVDPLNVMSMPRNV 232
Query: 225 TAATGLDALTHCVESYVS-------------------TAANPITDTCALKGVELIQEHLL 265
+G D L H +ES+ + +NPI+D + + + +I ++
Sbjct: 233 AIYSGFDVLCHALESFTALPFDQRSPRPENPGVRPLYQGSNPISDVWSKEALRIIGKYFR 292
Query: 266 NAFENGKDTTARTGMCYAQYLAGMAFNNASLGYVHSIAHQLGGFYH-------------L 312
+ + D ART M A AG+ F NA + H +++ + +
Sbjct: 293 RSIFDPTDEEARTEMLKASSFAGIGFGNAGVHLCHGLSYPISSQAKSCVADDYPKEKNLI 352
Query: 313 PHGVCNAVLLPHVQSFNKKDPRANER-------LSQIAPHLGAKDATADALVERLLEFTK 365
PHG+ +V+ V F +R L P+ + + + L +RL + +
Sbjct: 353 PHGL--SVMTTAVADFEFTTAACPDRHLISAQTLGADIPNNASNEYISRTLCDRLRGYMR 410
Query: 366 SLGIPRNLKELGVKEEDFEILAEHALKDVCGLTNPIQFTKDEVIAII 412
G+P LK +G + D E+L E A V + + E+I+ +
Sbjct: 411 DFGVPNGLKGMGFEFSDIEMLTEAASHSVPNIAISPKSADREIISTL 457
>7291405 [C] KOG3857 Alcohol dehydrogenase class IV
Length = 464
Score = 110 bits (276), Expect = 3e-24
Identities = 94/385 (24%), Positives = 156/385 (40%), Gaps = 46/385 (11%)
Query: 51 FGEGALDEVADYIKSQNFKRPVIITDPGISKIGLTDRVASLLEKRGSAVGIYDQTQPNPT 110
FG G EV +++ ++ ++TD + ++ L + G +YD+T+ PT
Sbjct: 53 FGPGVSAEVGADLRNLGARKVCLVTDKNVVQLPSVKVALDSLARNGINYEVYDETRVEPT 112
Query: 111 TSNVNAGLVVLKEHNXXXXXXXXXXXAHDNAKAIALLATNGG-EIGDYEGVNKSKNKAL- 168
++ + + A D AKA L +++ E DY + K +
Sbjct: 113 DGSMWHAVEFARGKEFDAFLAIGGGSAMDTAKAANLFSSDANAEFLDYVNCPIGRGKEIS 172
Query: 169 ----PMVAVNTTAGTASEMTKFTIITNEEKKVKMAIIDNHITPSVAVNDPSIMYGLPPSL 224
P++A+ TT+GT SE T I ++ K I + P++AV DP P +
Sbjct: 173 VKLKPLIAMPTTSGTGSETTGVAIFDYKKLHAKTGISSKFLKPTLAVIDPLHTLSQPQRV 232
Query: 225 TAATGLDALTHCVESYVST-------------------AANPITDTCALKGVELIQEHLL 265
A G D H +ES+ + NP++D A +E I+++ +
Sbjct: 233 MAFAGFDVFCHALESFTAVDYRERGLAPSDPSLRPTYQGRNPVSDVWARFALETIRKNFV 292
Query: 266 NAFENGKDTTARTGMCYAQYLAGMAFNNASLGYVHSIAHQLGGFYH-------------L 312
NA + AR+ M A +AG+ F NA + H +++ + G +
Sbjct: 293 NAIYQPDNLEARSQMHLASTMAGVGFGNAGVHLCHGLSYPISGNVRDYKPKGYSADHALI 352
Query: 313 PHGVCNAVLLPHVQSFNKKDPRANERLSQIAPHLGAK---DATADA---LVERLLEFTKS 366
PHG+ + P V F P +R + A LGA+ ADA L + + F +
Sbjct: 353 PHGLSVVISAPAVFEFTA--PACPDRHLEAAQLLGAEVRGVEKADAGRLLADTVRGFMQR 410
Query: 367 LGIPRNLKELGVKEEDFEILAEHAL 391
GI L+ELG D L E L
Sbjct: 411 AGIENGLRELGFSSSDIPALVEGTL 435
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.133 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,606,660
Number of Sequences: 60738
Number of extensions: 958826
Number of successful extensions: 2239
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2226
Number of HSP's gapped (non-prelim): 6
length of query: 418
length of database: 30,389,216
effective HSP length: 109
effective length of query: 309
effective length of database: 23,768,774
effective search space: 7344551166
effective search space used: 7344551166
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)