ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIV0128   suspect: LH   O     KOG2879  Posttranslational modification, protein turnover, chaperones   Predicted E3 ubiquitin ligase

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIV0128  38504  39235 244  
         (244 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YJL210w [O] KOG2879 Predicted E3 ubiquitin ligase                      87   3e-17

>YJL210w [O] KOG2879 Predicted E3 ubiquitin ligase
          Length = 271

 Score = 86.7 bits (213), Expect = 3e-17
 Identities = 72/279 (25%), Positives = 108/279 (37%), Gaps = 60/279 (21%)

Query: 1   MSRVAQLDGVSLDLQLTRALQSDLGFPCP--------QIVVEILVFLLGSRRW-HQGSVA 51
           MSRVAQLD ++LD +L     S+              ++ +  +VF+  +R   H GS  
Sbjct: 1   MSRVAQLDSIALDKELYGQFWSEFNAAFNTSEHKEEWELALNTVVFMCATRFLPHYGSSC 60

Query: 52  TYGSVLTGVGYQCSRWRLFAVTVVLRHL--------------------LHRFERTNLVYE 91
           TYGS L+GV +QC +  L+ VTV+  ++                    L  ++  NL+Y 
Sbjct: 61  TYGSALSGVVFQCRKRTLYVVTVLAGYVWKKITHIIFNGPHCGNQMMWLKLYKWVNLLYH 120

Query: 92  LLDLIHLLQFLTS-GTGGPSLLH-----------RLFRVPAVFQPGFXXXXXXXXXXXXE 139
             D+ + L+FL + G    + L            RL R  +     F             
Sbjct: 121 GCDVTNFLRFLAAEGPNARAFLSPLYRAFNVHSTRLIRDGSAIASEFYSNSVFAGLEYQN 180

Query: 140 VQMLWNASIEMIAGLSLFKNGFXXXXXXXXXXXXXXXXXXXXXXXXXXXXEKFSQKNTLC 199
            Q+LWNA +E+ +   L K G                                    T+C
Sbjct: 181 RQLLWNALLELFSNTLLTKRGLLTFVKKPPRSRSTTTY------------------KTVC 222

Query: 200 PVCGEVATNAFTITCCNTRYCYTCALE-VEQSHCLVCNT 237
           P CG   TN + I CC   YCY C ++ +E S C  C +
Sbjct: 223 PRCGGFPTNPYQIACCRANYCYVCVVKALEWSMCDACGS 261


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.329    0.141    0.453 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,073,693
Number of Sequences: 60738
Number of extensions: 424202
Number of successful extensions: 1261
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1259
Number of HSP's gapped (non-prelim): 2
length of query: 244
length of database: 30,389,216
effective HSP length: 103
effective length of query: 141
effective length of database: 24,133,202
effective search space: 3402781482
effective search space used: 3402781482
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)