ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV0132 good F KOG3721 Nucleotide transport and metabolism Mitochondrial endonuclease
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV0132 40289 39294 -332
(332 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL208c [F] KOG3721 Mitochondrial endonuclease 425 e-119
SPAC17C9.08 [F] KOG3721 Mitochondrial endonuclease 298 7e-81
7303509 [F] KOG3721 Mitochondrial endonuclease 216 4e-56
CE29488 [F] KOG3721 Mitochondrial endonuclease 196 5e-50
Hs4758270 [F] KOG3721 Mitochondrial endonuclease 188 1e-47
Hs4826714_1 [F] KOG3721 Mitochondrial endonuclease 156 5e-38
7299390 [F] KOG3721 Mitochondrial endonuclease 109 6e-24
7295984 [F] KOG3721 Mitochondrial endonuclease 59 8e-09
>YJL208c [F] KOG3721 Mitochondrial endonuclease
Length = 329
Score = 425 bits (1093), Expect = e-119
Identities = 197/263 (74%), Positives = 226/263 (85%), Gaps = 2/263 (0%)
Query: 65 NVDPGAFFKYGFPGPIHDLETRQEFISCYDRRTRNPYWVLEHITPESLKTKGADRKNSIF 124
NVDP FFKYGFPGPIHDL+ R+EFISCY+R+T+NPYWVLEHITPESL + ADRKNS F
Sbjct: 53 NVDPSGFFKYGFPGPIHDLQNREEFISCYNRQTQNPYWVLEHITPESLAARNADRKNSFF 112
Query: 125 KEDEQIPEMFRGRLKDYFRSGYDRGHQAPAANAKFSQQAMDDTFYLTNMCPQVGEGFNRD 184
KEDE IPE FRG+L+DYFRSGYDRGHQAPAA+AKFSQQAMDDTFYL+NMCPQVGEGFNRD
Sbjct: 113 KEDEVIPEKFRGKLRDYFRSGYDRGHQAPAADAKFSQQAMDDTFYLSNMCPQVGEGFNRD 172
Query: 185 YWAHLEYFCRQLVDKYNSVRIMTGPLYLPKRDPKDGKFKVTYEMIGNPPNIAVPTHFFKL 244
YWAHLEYFCR L KY SVRI+TGPLYLPK+DP D KF+V YE+IGNPP+IAVPTHFFKL
Sbjct: 173 YWAHLEYFCRGLTKKYKSVRIVTGPLYLPKKDPIDNKFRVNYEVIGNPPSIAVPTHFFKL 232
Query: 245 VVAERPKQLGNGRDDELHVAAFVLPNAPISNSTTLIEFEVPVNALERSSGLQLLQNVPKE 304
+VAE P N +++ VAAFVLPN PISN T L +FEVP++ALERS+GL+LLQ VP
Sbjct: 233 IVAEAP--TANPAREDIAVAAFVLPNEPISNETKLTDFEVPIDALERSTGLELLQKVPPS 290
Query: 305 KKKELCKQVRCEITVREFNNTVL 327
KKK LCK+V C+I VR+F+N +
Sbjct: 291 KKKALCKEVNCQIVVRDFSNAAI 313
>SPAC17C9.08 [F] KOG3721 Mitochondrial endonuclease
Length = 335
Score = 298 bits (763), Expect = 7e-81
Identities = 140/256 (54%), Positives = 191/256 (73%), Gaps = 5/256 (1%)
Query: 71 FFKYGFPGPIHDLETRQEFISCYDRRTRNPYWVLEHITPESLKTKGADRKNSIFKEDEQI 130
FF+YG PGP+ D ++S +DRRTRNP++ E IT ESL + +R+ S F D+ I
Sbjct: 63 FFQYGIPGPVADQRVNHGYMSVFDRRTRNPFYTAETITQESLNQRKGNRRYSEFVPDDNI 122
Query: 131 PEMFRGRLKDYFRSGYDRGHQAPAANAKFSQQAMDDTFYLTNMCPQVGEGFNRDYWAHLE 190
PEMF+ +L DY SGYDRGHQ PAA+ KFSQ+AM++TFYL+NMCPQVG+GFNR+YWA+ E
Sbjct: 123 PEMFQAKLGDYRGSGYDRGHQVPAADCKFSQEAMNETFYLSNMCPQVGDGFNRNYWAYFE 182
Query: 191 YFCRQLVDKYNSVRIMTGPLYLPKRDPKDGKFKVTYEMIGNPPNIAVPTHFFKLVVAERP 250
+CR+L KY SV IMTGPLYLPK++ + G+++V Y +IGNPPN+AVPTHFFK+++AE+
Sbjct: 183 DWCRRLTSKYGSVTIMTGPLYLPKKNER-GQWEVQYRVIGNPPNVAVPTHFFKVIIAEKS 241
Query: 251 KQLGNGRDDELHVAAFVLPNAPISNSTTLIEFEVPVNALERSSGLQLLQNVPKEKKKELC 310
+ + VAAFVLPN PI+++ L F VPV +ER+SGL++L NVPK +K+LC
Sbjct: 242 GEPTSSPS----VAAFVLPNKPIADNFPLKNFAVPVEVVERASGLEILSNVPKGNRKQLC 297
Query: 311 KQVRCEITVREFNNTV 326
+V C++ V+EF +V
Sbjct: 298 SEVVCQLNVKEFVESV 313
>7303509 [F] KOG3721 Mitochondrial endonuclease
Length = 310
Score = 216 bits (550), Expect = 4e-56
Identities = 114/252 (45%), Positives = 155/252 (61%), Gaps = 11/252 (4%)
Query: 55 QAGRVSLVGSNVDPGAFFKYGFPGPIHDLETRQEFISCYDRRTRNPYWVLEHITPESL-K 113
Q VSL + G KYGFPG H + + +++ YDRR R P+WV EH+T ES+ K
Sbjct: 60 QENNVSLTATPSRIGQIMKYGFPGLDH-VRSHSDYVLSYDRRNRVPHWVFEHLTAESVAK 118
Query: 114 TKGADRKNSIFKEDEQIPEMFRGRLKDYFRSGYDRGHQAPAANAKFSQQAMDDTFYLTNM 173
DR FK+DE I FR + DY RSGYDRGH A A N + Q+ D+TFYL+NM
Sbjct: 119 NDAVDRSKCDFKQDESIHPFFRSQNTDYRRSGYDRGHMAAAGNHRLHQKHCDETFYLSNM 178
Query: 174 CPQVGEGFNRDYWAHLEYFCRQLVDKYNSVRIMTGPLYLPKRDPKDGKFKVTYEMIGNPP 233
PQVG+GFNRD W LE R+L Y++V + TGPLYLP ++ DGK V YE+IG
Sbjct: 179 APQVGQGFNRDAWNTLEAHVRRLTKTYSNVYVCTGPLYLPHKE-DDGKSYVKYEVIG-AN 236
Query: 234 NIAVPTHFFKLVVAERPKQLGNGRDDELHVAAFVLPNAPISNSTTLIEFEVPVNALERSS 293
+AVPTHF+K++V G D +LH+ ++V+PN ISN T + F+VP ++ERS+
Sbjct: 237 TVAVPTHFYKVIV-------GESADHKLHMESYVMPNQVISNDTPISVFQVPPESVERSA 289
Query: 294 GLQLLQNVPKEK 305
GL + +++
Sbjct: 290 GLLFFDQINRKQ 301
>CE29488 [F] KOG3721 Mitochondrial endonuclease
Length = 308
Score = 196 bits (497), Expect = 5e-50
Identities = 107/238 (44%), Positives = 152/238 (62%), Gaps = 12/238 (5%)
Query: 73 KYGFPGPIHDLETRQEFISCYDRRTRNPYWVLEHITPESLK-TKGADRKNSIFKEDEQIP 131
K+G+PG ++ T ++F+ YD +TR +WV EH+TPE LK +G DRK FK D P
Sbjct: 71 KHGYPG-FTNVRTYEDFVLSYDYKTRTAHWVCEHLTPERLKHAEGVDRKLCEFKPDITFP 129
Query: 132 EMFRGRLKDYFRSGYDRGHQAPAANAKFSQQAMDDTFYLTNMCPQVGEGFNRDYWAHLEY 191
+ F + DY SG+DRGH A A N + SQ A+D TFYL+NM PQVG GFNRD W LE
Sbjct: 130 QKFLSQNTDYKCSGFDRGHLAAAGNHRKSQLAVDQTFYLSNMSPQVGRGFNRDKWNDLEM 189
Query: 192 FCRQLVDKYNSVRIMTGPLYLPKRDPKDGKFKVTYEMIGNPPNIAVPTHFFKLVVAERPK 251
CR++ K + I+TGPLYLPK + DGK + Y++IG+ N+AVPTHFFK+ + E
Sbjct: 190 HCRRVAKKMINSYIITGPLYLPKLE-GDGKKYIKYQVIGD-NNVAVPTHFFKVALFE--- 244
Query: 252 QLGNGRDDELHVAAFVLPNAPISNSTTLIEFEVPVNALERSSGLQLLQNV-PKEKKKE 308
+ + +++LPNA I ++ + +F VP++A+ERS+GL++ + PK KE
Sbjct: 245 ----VTPGKFELESYILPNAVIEDTVEISKFHVPLDAVERSAGLEIFARLDPKSIVKE 298
>Hs4758270 [F] KOG3721 Mitochondrial endonuclease
Length = 297
Score = 188 bits (477), Expect = 1e-47
Identities = 99/234 (42%), Positives = 145/234 (61%), Gaps = 12/234 (5%)
Query: 68 PGAFFKYGFPGPIHDLETRQEFISCYDRRTRNPYWVLEHITPESLKTKGADRKNSIFKED 127
PG KYG PG + L++R+ ++ CYD RTR WV+E + PE L+ G DR+ F+ED
Sbjct: 61 PGELAKYGLPG-LAQLKSRESYVLCYDPRTRGALWVVEQLRPERLRGDG-DRRECDFRED 118
Query: 128 EQIPEMFRGRLKDYFRSGYDRGHQAPAANAKFSQQAMDDTFYLTNMCPQVGEGFNRDYWA 187
+ + R DY SG+DRGH A AAN ++SQ+AMDDTFYL+ + PQV N++ W
Sbjct: 119 DSVHAYHRATNADYRGSGFDRGHLAAAANHRWSQKAMDDTFYLSKVAPQVPH-LNQNAWN 177
Query: 188 HLEYFCRQLVDKYNSVRIMTGPLYLPKRDPKDGKFKVTYEMIGNPPNIAVPTHFFKLVVA 247
+LE + R L Y +V + TGPL+LP R DGK V Y++IG ++AVPTHFFK+++
Sbjct: 178 NLEKYSRSLTRSYQNVYVCTGPLFLP-RTEADGKSYVKYQVIGK-NHVAVPTHFFKVLIL 235
Query: 248 ERPKQLGNGRDDELHVAAFVLPNAPISNSTTLIEFEVPVNALERSSGLQLLQNV 301
E ++ + +V+PNAP+ + L F VP+ ++ER+SGL + N+
Sbjct: 236 E-------AAGGQIELRTYVMPNAPVDEAIPLERFLVPIESIERASGLLFVPNI 282
>Hs4826714_1 [F] KOG3721 Mitochondrial endonuclease
Length = 308
Score = 156 bits (394), Expect = 5e-38
Identities = 96/268 (35%), Positives = 144/268 (52%), Gaps = 13/268 (4%)
Query: 55 QAGRVSLVGSNVDPGA----FFKYGFPGPIHDLETRQEFISCYDRRTRNPYWVLEHITPE 110
Q +L G D A ++GFP + YD+ R P WVLEHI+
Sbjct: 42 QGAEGALTGKQPDGSAEKAVLEQFGFPLTGTEARCYTNHALSYDQAKRVPRWVLEHISKS 101
Query: 111 SLKTKGADRKNSIFKEDEQIPEMFRGRLKDYFRSGYDRGHQAPAANAKFSQQAMDDTFYL 170
+ ADRK+ FK D IP F +DY SG+ RGH APA N KFS +AM +TFYL
Sbjct: 102 KIMGD-ADRKHCKFKPDPNIPPTFSAFNEDYVGSGWSRGHMAPAGNNKFSSKAMAETFYL 160
Query: 171 TNMCPQVGEGFNRDYWAHLEYFCRQLVDKYNSVRIMTGPLYLPKRDPKDGKFKVTYEMIG 230
+N+ PQ + N YW +E +CR+L +++ V +++GPL LP + DGK V+Y++IG
Sbjct: 161 SNIVPQDFDN-NSGYWNRIEMYCRELTERFEDVWVVSGPLTLP-QTRGDGKKIVSYQVIG 218
Query: 231 NPPNIAVPTHFFKLVVAERPKQLGNGRDDELHVAAFVLPNAPISNSTTLIEFEVPVNALE 290
N+AVP+H +K+++A R + + L + AFV+PN I L EF+V + LE
Sbjct: 219 E-DNVAVPSHLYKVILARR----SSVSTEPLALGAFVVPNEAIGFQPQLTEFQVSLQDLE 273
Query: 291 RSSGLQLLQNVPKEKK-KELCKQVRCEI 317
+ S L ++ + + +C C++
Sbjct: 274 KLSVLVFFPHLDRTSDIRNICSVDTCKL 301
>7299390 [F] KOG3721 Mitochondrial endonuclease
Length = 242
Score = 109 bits (272), Expect = 6e-24
Identities = 68/240 (28%), Positives = 116/240 (48%), Gaps = 21/240 (8%)
Query: 73 KYGFPGPIHDLETRQEFISCYDRRTRNPYWVLEHITPESLKTKGADRKN--SIFKEDEQI 130
KYGFPG + ++ + F+ YDRR R +WV EH+ ++ + N + + D I
Sbjct: 2 KYGFPG-MDEIHVYKNFVLSYDRRNRIAHWVCEHVKSGCVQERDEKTLNKPNAYITDNTI 60
Query: 131 PEMFRGRLKDYFRSGYDRGHQAPAANAKFSQQAMDDTFYLTNMCPQVGEGFNRDYWAHLE 190
P +F ++D+ S + GH A N K + + TN+ P + G W LE
Sbjct: 61 PTIFSANMRDFKNSDWVGGHLASPQNYKCDALKFLEAYKFTNIVP-INRGLKNHIWYRLE 119
Query: 191 YFCRQLVDKYNSVRIMTGPLYLPKRDPKDGKFKVTYEMIGNPPNIAVPTHFFKLVVAERP 250
+ R +++SV + TGPL++P+R + V Y ++G +AVPTHFFK+++
Sbjct: 120 SYVRDKAIEFDSVHVYTGPLFMPQR-ITFRNWSVRYHVMG-MNTVAVPTHFFKIII---- 173
Query: 251 KQLGNGRDDELH-----VAAFVLPNAPISNSTTLIEFEVPVNALERSSGLQLLQNVPKEK 305
R+DE + + +V+PNA + L F V +E +GL+ +++
Sbjct: 174 ------REDEFNRDMPIMEGYVVPNAYVDKDMDLRSFLADVRDIEHFAGLKFCDGQQRDQ 227
>7295984 [F] KOG3721 Mitochondrial endonuclease
Length = 217
Score = 59.3 bits (142), Expect = 8e-09
Identities = 46/179 (25%), Positives = 77/179 (42%), Gaps = 37/179 (20%)
Query: 73 KYGFPGPIHDLETRQ--EFISCYDRRTRNPYWVLEHITPESLKTKGADRKNSIFKEDEQI 130
KYGFP +D+ + +F++ +DRR W+ E + D N + D
Sbjct: 2 KYGFPST-NDITINETFDFVTSFDRRNSAILWMCERV----------DLSNRVVYGDST- 49
Query: 131 PEMFRGRLKDYFRSGYDRGHQAPAANAKFSQQAMDDTFYLTNMCPQVGEGFNRDYWAHLE 190
APA F Q F+L+N+ P + GFN W L
Sbjct: 50 -------------------SVAPAG--AFGQSEAARVFFLSNIRPFLNRGFNLTVWDRLL 88
Query: 191 YFCRQLVDKYNSVRIMTGPLYLPKRDPKDGKFKVTYEMIGNPPNIAVPTHFFKLVVAER 249
+ ++ ++ +V TG +YLP R+ K + + ++ +AVPTHFFK++V ++
Sbjct: 89 QYVHEMSQRHGTVYAYTGSIYLP-RELKSNSWFLEFQS-EERTMVAVPTHFFKILVIDK 145
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.138 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,122,006
Number of Sequences: 60738
Number of extensions: 877102
Number of successful extensions: 1748
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1717
Number of HSP's gapped (non-prelim): 9
length of query: 332
length of database: 30,389,216
effective HSP length: 107
effective length of query: 225
effective length of database: 23,890,250
effective search space: 5375306250
effective search space used: 5375306250
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)