ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIV0219   suspect: LH   U     KOG0946  Intracellular trafficking, secretion, and vesicular transport  ER-Golgi vesicle-tethering protein p115

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIV0219  69450 67747 -568 
         (568 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDL058w [U] KOG0946 ER-Golgi vesicle-tethering protein p115            54   5e-07

>YDL058w [U] KOG0946 ER-Golgi vesicle-tethering protein p115
          Length = 1790

 Score = 54.3 bits (129), Expect = 5e-07
 Identities = 94/429 (21%), Positives = 181/429 (41%), Gaps = 75/429 (17%)

Query: 88   QLFNGIKTIEKVLLHLMEIIEEEFGCNEDEL-FQKELTALFDLLEEISDTLVSIKP---- 142
            +L   ++T EK L    E+ E E    E+++  +KE T     L  +   L S++     
Sbjct: 1100 ELETKLETSEKAL---KEVKENEEHLKEEKIQLEKEATETKQQLNSLRANLESLEKEHED 1156

Query: 143  IISSLKNLFDTAIEFNEIFKDHMNSLDEEIENNLSKCIELQEQNFESPVRHNKPTFTLDQ 202
            + + LK   +        + + ++ L++EI +   +   ++++N E           L+ 
Sbjct: 1157 LAAQLKKYEEQIANKERQYNEEISQLNDEITSTQQENESIKKKNDE-----------LEG 1205

Query: 203  LIKTLSSTNSPTKGLQIPAFSPSEQKIHEKLTQLEDSSAPIDLSLKHVLSERLD------ 256
             +K + ST+     L+         +I E   + E + A +  S+K V SE +       
Sbjct: 1206 EVKAMKSTSEEQSNLKKSEIDALNLQIKELKKKNETNEASLLESIKSVESETVKIKELQD 1265

Query: 257  --NFETRDVVNLEYLMRLLRKKYQYILDKYELLQVELRDLKQAIVDEKWVLIFTTLNDEL 314
              NF+ ++V  LE  ++    K      KY  LQ E   +K+ + D K          EL
Sbjct: 1266 ECNFKEKEVSELEDKLKASEDKNS----KYLELQKESEKIKEEL-DAK--------TTEL 1312

Query: 315  RIMLKDVEKL--LLKVGNPDLSEQLKT-------------KLHDQLRSKSYTIDKTFGII 359
            +I L+ +  L    +    +LS   KT             KL ++++ K+   +K     
Sbjct: 1313 KIQLEKITNLSKAKEKSESELSRLKKTSSEERKNAEEQLEKLKNEIQIKNQAFEK----- 1367

Query: 360  YNALESSILNESIATVTNEHAEKWLQLNTKFEHLLPEHQNEND-PNKTITQLSNDISELR 418
                E  +LNE  +T+T E++EK   +NT  + L+   QNEN+   K I    +++ ++ 
Sbjct: 1368 ----ERKLLNEGSSTITQEYSEK---INTLEDELI-RLQNENELKAKEIDNTRSELEKVS 1419

Query: 419  LNDAPSEKPGSNRSSIGAILFKKMNIKPVLVREGDENDSNSDNVKCDRHRYSNPFFDPCQ 478
            L++   E     +++I ++  + ++ K  + R    ND    +++ D  R      +  +
Sbjct: 1420 LSN--DELLEEKQNTIKSLQDEILSYKDKITR----NDEKLLSIERDNKRDLESLKEQLR 1473

Query: 479  ATKLSPKKV 487
            A + S  KV
Sbjct: 1474 AAQESKAKV 1482


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.316    0.134    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,657,717
Number of Sequences: 60738
Number of extensions: 1485341
Number of successful extensions: 5070
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 5070
Number of HSP's gapped (non-prelim): 1
length of query: 568
length of database: 30,389,216
effective HSP length: 112
effective length of query: 456
effective length of database: 23,586,560
effective search space: 10755471360
effective search space used: 10755471360
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)