ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV0283 good R KOG3164 General function prediction only Uncharacterized proteins of PilT N-term./Vapc superfamily
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV0283 89887 90690 268
(268 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR004w [R] KOG3164 Uncharacterized proteins of PilT N-term./Vap... 287 9e-78
SPCC18.12c [R] KOG3164 Uncharacterized proteins of PilT N-term./... 191 7e-49
7296046 [R] KOG3164 Uncharacterized proteins of PilT N-term./Vap... 104 1e-22
CE27743 [R] KOG3164 Uncharacterized proteins of PilT N-term./Vap... 94 3e-19
Hs14150118 [R] KOG3164 Uncharacterized proteins of PilT N-term./... 87 3e-17
At2g34570 [R] KOG3164 Uncharacterized proteins of PilT N-term./V... 82 8e-16
At2g46230 [R] KOG3165 Predicted nucleic-acid-binding protein con... 66 6e-11
YDR339c [R] KOG3165 Predicted nucleic-acid-binding protein conta... 65 1e-10
Hs7705730 [R] KOG3165 Predicted nucleic-acid-binding protein con... 64 2e-10
SPBC32H8.04c [R] KOG3165 Predicted nucleic-acid-binding protein ... 54 2e-07
ECU05g0560 [R] KOG3165 Predicted nucleic-acid-binding protein co... 53 4e-07
CE09810 [R] KOG3165 Predicted nucleic-acid-binding protein conta... 50 4e-06
>YOR004w [R] KOG3164 Uncharacterized proteins of PilT N-term./Vapc
superfamily
Length = 254
Score = 287 bits (735), Expect = 9e-78
Identities = 143/215 (66%), Positives = 177/215 (81%), Gaps = 5/215 (2%)
Query: 1 MRQKRAKSYRKQMLVYNHTFKFRQPYQVLVDDQLVLETFNSSFDFVKGLQRTLQAEVKPM 60
MRQKRAKSYRKQ+LVY+HTFKFR+PYQVLVD+QLVLE NS+F+ GL+RTLQA+VK M
Sbjct: 1 MRQKRAKSYRKQLLVYSHTFKFREPYQVLVDNQLVLECNNSNFNLPSGLKRTLQADVKVM 60
Query: 61 ITQCCMQSLYQTNNQGAIDAGKQFERRRCNHDPKEPKSVLECLTSVVDVNGKNKHRYVVA 120
ITQCC+Q+LY+T N GAI+ KQFERRRCNH K+PKS EC+ SVV+++G NKHRYVVA
Sbjct: 61 ITQCCIQALYETRNDGAINLAKQFERRRCNHSFKDPKSPAECIESVVNISGANKHRYVVA 120
Query: 121 TQDVEIRRRLRKIPGVPLVYMNRSVMVMEPLSNASEKVSREVEEQKLYKGLNDPKFAGIA 180
+QD+++RR+LR +PGVPL+++ RSVMVMEPLS AS K S+ EEQKLYKGLNDP +
Sbjct: 121 SQDIDLRRKLRTVPGVPLIHLTRSVMVMEPLSTASAKASKITEEQKLYKGLNDPNIEKL- 179
Query: 181 RDENDEAGAENQENKPKKRK-GPKEPNPLSMKKKK 214
++ D +G +E+ KKRK GPK PNPLS+KKKK
Sbjct: 180 QESGDGSG---KESITKKRKLGPKAPNPLSVKKKK 211
>SPCC18.12c [R] KOG3164 Uncharacterized proteins of PilT N-term./Vapc
superfamily
Length = 260
Score = 191 bits (486), Expect = 7e-49
Identities = 111/230 (48%), Positives = 143/230 (61%), Gaps = 18/230 (7%)
Query: 1 MRQKRAKSYRKQMLVYNHTFKFRQPYQVLVDDQLVLETFNSSFDFVKGLQRTLQAEVKPM 60
MRQKRAK+YRK M Y F FR+PYQVLVD + + D L RT+Q +KPM
Sbjct: 1 MRQKRAKNYRKLMHTYQLLFGFREPYQVLVDADFLKDLSQQKIDIQAALARTVQGAIKPM 60
Query: 61 ITQCCMQSLYQTNNQ--GAIDAGKQFERRRCNHDPKEPKSVLECLTSVVDVNGKNKHRYV 118
ITQCC++ LY +++ I K FERRRC H E S EC+ SVV++NG+NKHRYV
Sbjct: 61 ITQCCIRQLYSKSDELKQEIRIAKSFERRRCGHID-EALSPSECIQSVVNINGRNKHRYV 119
Query: 119 VATQDVEIRRRLRKIPGVPLVYMNRSVMVMEPLSNAS--EKVSRE------VEEQKLYKG 170
VATQD E+R+ LR +PGVPL+YM RSV+++EP S A+ EK ++E +E+KL
Sbjct: 120 VATQDPELRQALRSVPGVPLIYMKRSVVILEPASRATLLEKHNKESVQMGMSKEEKLLLS 179
Query: 171 LNDPKFAGIARDENDE------AGAENQENKP-KKRKGPKEPNPLSMKKK 213
+A D+ D AG E+ K KKRKGPK PNPLS+KK+
Sbjct: 180 GKKRSANELAIDDQDTKESTDLAGTEDSAPKANKKRKGPKGPNPLSIKKR 229
>7296046 [R] KOG3164 Uncharacterized proteins of PilT N-term./Vapc
superfamily
Length = 244
Score = 104 bits (260), Expect = 1e-22
Identities = 73/219 (33%), Positives = 107/219 (48%), Gaps = 17/219 (7%)
Query: 1 MRQKRAKSYRKQMLVYNHTFKFRQPYQVLVDDQLVLETFNSSFDFVKGLQRTLQAEVKPM 60
M+ R K K ++ + F +R+PYQVL+D + +++ Q VK +
Sbjct: 1 MKISRFKKSHKTLVFFASNFDYREPYQVLIDATFCQAALQQKIGIDEQIKKYFQCGVKLL 60
Query: 61 ITQCCMQSLYQTNNQ--GAIDAGKQFERRRCNHDPKEPKSVLECLTSVVDVNGKNKHRYV 118
TQC + GA K+F +C H+ K P EC+ S+ N RYV
Sbjct: 61 TTQCVILESESLGAPLTGATSIVKRFHVHKCGHEGK-PVPASECIKSMTKDN-----RYV 114
Query: 119 VATQDVEIRRRLRKIPGVPLVYMNRSVMVMEPLSNASEK-VSREVEEQKLYKGLNDPKFA 177
VA+QD ++ LRKIPG L+Y++++ V+E S AS+K V R + L K + +
Sbjct: 115 VASQDRLLQESLRKIPGRCLLYLHKATPVLEAPSKASKKWVQRRAKNLMLGKQVEKIDYM 174
Query: 178 GIARDENDEAGAENQEN--KPKKRKGPKEPNPLSMKKKK 214
++ G + E KPKK KGPK PNPLS KK K
Sbjct: 175 ------KEKQGLKPAETAVKPKKHKGPKNPNPLSCKKSK 207
>CE27743 [R] KOG3164 Uncharacterized proteins of PilT N-term./Vapc
superfamily
Length = 232
Score = 93.6 bits (231), Expect = 3e-19
Identities = 66/216 (30%), Positives = 101/216 (46%), Gaps = 20/216 (9%)
Query: 1 MRQKRAKSYRKQMLVYNHTFKFRQPYQVLVDDQLVLETFNSSFDFVKGLQRTLQAEVKPM 60
M+ KR K + + Y + +KF PY+VLVD + + + + L E M
Sbjct: 1 MKVKRLKRANRLLTFYKYNYKFVAPYRVLVDGTFCNAALQEKLNLAEQIPKYLTEETHLM 60
Query: 61 ITQCCMQSLYQTNN--QGAIDAGKQFERRRCNHDPKEPKSVLECLTSVVDVNGKNKHRYV 118
T C ++ L + GA KQFE C H P++ +CL + K +Y+
Sbjct: 61 TTNCVLKELEKFGPLLYGAFVIAKQFEIAECTHST--PRAASDCLAHLARRAASGKTKYL 118
Query: 119 VATQDVEIRRRLRKIPGVPLVYMNRSVMVMEPLSNASEK-VSREVEEQKLYKGLNDPKFA 177
+ATQD E+ +LR I G P++Y+ ++++ +S A++ S+E E K K L +
Sbjct: 119 IATQDDELTEKLRAIVGTPIMYIKFKTVLLDNISEATKAGCSKEESEIKKLKELKN---- 174
Query: 178 GIARDENDEAGAENQENKPKKRKGPKEPNPLSMKKK 213
A QE K KK+K K NPLS KKK
Sbjct: 175 ---------ALIGQQELKKKKKK--KGVNPLSCKKK 199
>Hs14150118 [R] KOG3164 Uncharacterized proteins of PilT N-term./Vapc
superfamily
Length = 249
Score = 86.7 bits (213), Expect = 3e-17
Identities = 63/216 (29%), Positives = 101/216 (46%), Gaps = 7/216 (3%)
Query: 1 MRQKRAKSYRKQMLVYNHTFKFRQPYQVLVDDQLVLETFNSSFDFVKGLQRTLQAEVKPM 60
M+ R K +K + + + F R+PYQ+L+D + L R L E +
Sbjct: 1 MKITRQKHAKKHLGFFRNNFGVREPYQILLDGTFCQAALRGRIQLREQLPRYLMGETQLC 60
Query: 61 ITQCCMQSLYQTNNQ--GAIDAGKQFERRRCNHDPKEPKSVLECLTSVVDVNGKNKHRYV 118
T+C ++ L GA ++ + R C H K S ECL S+V+ N H Y
Sbjct: 61 TTRCVLKELETLGKDLYGAKLIAQKCQVRNCPHF-KNAVSGSECLLSMVEEG--NPHHYF 117
Query: 119 VATQDVEIRRRLRKIPGVPLVYMNRSVMVMEPLSNASEKVSREVEEQKLYKGLNDPKFAG 178
VATQD + +++K PGVPL+++ ++ MV++ S + + VE +L
Sbjct: 118 VATQDQNLSVKVKKKPGVPLMFIIQNTMVLDKPSPKTIAFVKAVESGQLVSVHEKESIKH 177
Query: 179 IARDENDEAGAENQENKPKKRKGPKEPNPLSMKKKK 214
+ ++ E +++ KKRK PNPLS KKK
Sbjct: 178 LKEEQGLVKNTE--QSRRKKRKKISGPNPLSCLKKK 211
>At2g34570 [R] KOG3164 Uncharacterized proteins of PilT N-term./Vapc
superfamily
Length = 258
Score = 82.0 bits (201), Expect = 8e-16
Identities = 68/239 (28%), Positives = 110/239 (45%), Gaps = 29/239 (12%)
Query: 1 MRQKRAKSYRKQMLVYNHTFKFRQPYQVLVDDQLVLETFNSSFDFVK-GLQRTLQAEVKP 59
MR KR K R+ + + + FRQPY+VL D V + + L VK
Sbjct: 1 MRVKRQKKNRRTVRFFTVCYGFRQPYKVLCDGTFVHHLVTNEITPADTAVSELLGGPVKL 60
Query: 60 MITQCCMQSLYQTNNQGA--IDAGKQFERRRCNHDPKEPKSVLECLTSVVDVNGKNKHRY 117
T+C + L + A ++A + C H+ E K+ ECL+ V+ V +N +
Sbjct: 61 FTTRCVIAELEKLGKDFAESLEAAQTLNTATCEHE--EAKTADECLSEVIGV--QNTEHF 116
Query: 118 VVATQDVEIRRRLRKIPGVPLVYMNRSVMVMEPLSNASEKVSREVEEQKL-----YKGLN 172
+ TQD E RR+L++ VPLV+ R++++++ S+ + +++ E ++L K L
Sbjct: 117 FLGTQDAEFRRKLQQESIVPLVFGLRNILLIDQPSDFQRQSAKDSENKRLTMTDTEKKLL 176
Query: 173 DPKFAGIARDENDEAGAENQE----------------NKPK-KRKGPKEPNPLSMKKKK 214
+ A I EA N+E ++P+ KR K PNPLS KKK
Sbjct: 177 VKRTAKIIASNRKEATIANEEWGMPRVVSTKNGLGVKDRPQFKRNRAKGPNPLSCMKKK 235
>At2g46230 [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 216
Score = 65.9 bits (159), Expect = 6e-11
Identities = 45/147 (30%), Positives = 70/147 (47%), Gaps = 13/147 (8%)
Query: 14 LVYNHTFKFRQPYQVLVDDQLVLETFNSSFDFVKGLQRTLQAEVKPMITQCCMQSLYQTN 73
L +++ PY+VLVD + + + D KG++ L A P IT C M L +
Sbjct: 51 LFFSYNSTLVPPYRVLVDTNFINFSIQNKIDLEKGMRDCLYANCTPCITDCVMAELEKLG 110
Query: 74 NQGA----IDAGKQFERRRCNHDPKEPKSVLECLTSVVDVNGKNKHR-YVVATQDVEIRR 128
+ I FER C H +CL V +H+ ++VAT D +++R
Sbjct: 111 QKYRVALRIAKDPHFERLPCIHKGTYAD---DCLVDRV-----TQHKCFIVATCDRDLKR 162
Query: 129 RLRKIPGVPLVYMNRSVMVMEPLSNAS 155
R+RKIPGVP++Y+ +E L A+
Sbjct: 163 RIRKIPGVPIMYVTNRKYSIEKLPEAT 189
>YDR339c [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 189
Score = 64.7 bits (156), Expect = 1e-10
Identities = 42/143 (29%), Positives = 71/143 (49%), Gaps = 13/143 (9%)
Query: 14 LVYNHTFKFRQPYQVLVDDQLVLETFNSSFDFVKGLQRTLQAEVKPMITQCCMQSLYQTN 73
L + + + PYQVL+D + + D V+G+ L A+ P+IT C M L +
Sbjct: 51 LFFQYNQAIKPPYQVLIDTNFINFSIQKKVDIVRGMMDCLLAKCNPLITDCVMAELEKLG 110
Query: 74 NQGAI----DAGKQFERRRCNHDPKEPKSVLECLTSVVDVNGKNKHR-YVVATQDVEIRR 128
+ I + +R C+H + +CL V+ +H+ Y+VAT D +++
Sbjct: 111 PKYRIALKLARDPRIKRLSCSH---KGTYADDCL-----VHRVLQHKCYIVATNDAGLKQ 162
Query: 129 RLRKIPGVPLVYMNRSVMVMEPL 151
R+RKIPG+PL+ + V+E L
Sbjct: 163 RIRKIPGIPLMSVGGHAYVIEKL 185
>Hs7705730 [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 198
Score = 63.9 bits (154), Expect = 2e-10
Identities = 40/134 (29%), Positives = 66/134 (48%), Gaps = 13/134 (9%)
Query: 14 LVYNHTFKFRQPYQVLVDDQLVLETFNSSFDFVKGLQRTLQAEVKPMITQCCMQSLYQTN 73
L + + + PY +LVD + + + D V+ + L A+ P IT C M + +
Sbjct: 55 LFFQYNTQLGPPYHILVDTNFINFSIKAKLDLVQSMMDCLYAKCIPCITDCVMAEIEKLG 114
Query: 74 NQGA----IDAGKQFERRRCNHDPKEPKSVLECLTSVVDVNGKNKHR-YVVATQDVEIRR 128
+ I +FER C H +CL V +H+ Y+VAT D +++R
Sbjct: 115 QKYRVALRIAKDPRFERLPCTHKGTYAD---DCLVQRV-----TQHKCYIVATVDRDLKR 166
Query: 129 RLRKIPGVPLVYMN 142
R+RKIPGVP++Y++
Sbjct: 167 RIRKIPGVPIMYIS 180
>SPBC32H8.04c [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 192
Score = 54.3 bits (129), Expect = 2e-07
Identities = 38/132 (28%), Positives = 62/132 (46%), Gaps = 17/132 (12%)
Query: 17 NHTFKFRQ----PYQVLVDDQLVLETFNSSFDFVKGLQRTLQAEVKPMITQCCMQSLYQT 72
N F+F + PY V++D + D +GL L A+ P I+ C M L +
Sbjct: 52 NLFFQFNESLGPPYHVIIDTNFINFCLQQKIDLFEGLMTCLYAKTIPCISDCVMAELEKL 111
Query: 73 NNQGAIDA----GKQFERRRCNHDPKEPKSVLECLTSVVDVNGKNKHR-YVVATQDVEIR 127
+ I ++FER C H +C+ V +H+ Y+VAT D ++
Sbjct: 112 GIRYRIALRIAKDERFERLPCTHKGTYAD---DCIVQRV-----MQHKCYLVATNDKNLK 163
Query: 128 RRLRKIPGVPLV 139
+R+RKIPG+P++
Sbjct: 164 QRIRKIPGIPIL 175
>ECU05g0560 [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 179
Score = 53.1 bits (126), Expect = 4e-07
Identities = 34/128 (26%), Positives = 58/128 (44%), Gaps = 15/128 (11%)
Query: 17 NHTFKFRQPYQVLVDDQLVLETFNSSFDFVKGLQRTLQAEVKPMITQCCMQSLYQTNNQG 76
NHT R PY +++D + + +D + L + + A +K I C + L
Sbjct: 45 NHTL--RPPYNIILDTNFINDCIRKKYDIREQLMKAVNASIKICIPDCVIGELESLGRPF 102
Query: 77 AIDAGK----QFERRRCNHDPKEPKSVLECLTSVVDVNGKNKHR-YVVATQDVEIRRRLR 131
+ +R C+H +C+ N + HR Y+VAT DV +++R++
Sbjct: 103 RVALASIRDSDVQRLECDHKGTYAD---DCI-----FNRVSAHRCYIVATSDVALKQRIK 154
Query: 132 KIPGVPLV 139
IPGVPL+
Sbjct: 155 AIPGVPLI 162
>CE09810 [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 196
Score = 49.7 bits (117), Expect = 4e-06
Identities = 38/134 (28%), Positives = 64/134 (47%), Gaps = 12/134 (8%)
Query: 25 PYQVLVDDQLVLETFNSSFDFVKGLQRTLQAEVKPMITQCCMQSLYQTNN-QGAIDAGK- 82
P+ V+VD V + D +G L A+ T C + L + + A+ K
Sbjct: 65 PFHVIVDTNFVNFAVKNRIDMFQGFMDCLFAKTIVYATDCVLAELEKVRRFKIALKVLKD 124
Query: 83 -QFERRRCNHDPKEPKSVLECLTSVVDVNGKNKHR-YVVATQDVEIRRRLRKIPGVPLVY 140
+ +R +C H +CL V +H+ Y+VAT D +++RR+RKIPGVP++Y
Sbjct: 125 PRVQRLKCEHKGTYAD---DCLVQRV-----TQHKCYIVATCDRDLKRRIRKIPGVPIMY 176
Query: 141 MNRSVMVMEPLSNA 154
+ +E + +A
Sbjct: 177 IVNHRFSIERMPDA 190
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.131 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,132,918
Number of Sequences: 60738
Number of extensions: 564691
Number of successful extensions: 1699
Number of sequences better than 1.0e-05: 12
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 1680
Number of HSP's gapped (non-prelim): 12
length of query: 268
length of database: 30,389,216
effective HSP length: 105
effective length of query: 163
effective length of database: 24,011,726
effective search space: 3913911338
effective search space used: 3913911338
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)