ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV0297 good P KOG3789 Inorganic ion transport and metabolism Nitrogen permease regulator NLRG/NPR2
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV0297 96096 94117 -660
(660 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YEL062w [P] KOG3789 Nitrogen permease regulator NLRG/NPR2 520 e-147
SPAC23H3.03c [P] KOG3789 Nitrogen permease regulator NLRG/NPR2 173 7e-43
Hs5729949 [P] KOG3789 Nitrogen permease regulator NLRG/NPR2 132 1e-30
7293296 [P] KOG3789 Nitrogen permease regulator NLRG/NPR2 109 2e-23
CE28406_2 [P] KOG3789 Nitrogen permease regulator NLRG/NPR2 90 1e-17
>YEL062w [P] KOG3789 Nitrogen permease regulator NLRG/NPR2
Length = 624
Score = 520 bits (1340), Expect = e-147
Identities = 292/678 (43%), Positives = 403/678 (59%), Gaps = 81/678 (11%)
Query: 1 MTSTFDGFVPIHTVFYTLFHPTEGSKVRFQFPPNSLENGKIDFDTIKNYIIPKPQLCNKL 60
M S F GFVPIHT+FY++FHPTEGSK++++FPPN+L+N I+F+T KNYIIPKP LC+KL
Sbjct: 10 MLSYFQGFVPIHTIFYSVFHPTEGSKIKYEFPPNNLKNHGINFNTFKNYIIPKPILCHKL 69
Query: 61 LTFKYGEYRIVCYPVNVNARYYARNSXXXXXXXXXPYDCATSPYEPAIARLGKMFKVLEE 120
+TFKYG YRIVCYPV +N+ YARN PYDC TSPYEPAI RLGKMFKVLEE
Sbjct: 70 ITFKYGTYRIVCYPVTINSPIYARNFFSFNFVFVFPYDCETSPYEPAITRLGKMFKVLEE 129
Query: 121 QSQILSKSEQNPVYFQLKSNDSNAADQSTEMGSITISEHTDIKSTGDDGEK--------- 171
Q+Q+LSKSE++PV+F LK +++ ST S T + ++ T EK
Sbjct: 130 QNQLLSKSERDPVFFDLKVLENSTTTPSTAGPSSTPNPSSNTTPTHPTSEKDTKDMRSSR 189
Query: 172 YLEIIKELAKNQKSLIIEDLLMKLFLDLNNYSECLIPIDEGNSIDIKLFPVRTPPSSNIS 231
Y ++IK+L Q + I+DLLM++F DLNNYSECLIPIDEGN++DIK+FP+ PP++ +S
Sbjct: 190 YSDLIKDLGLPQSAFSIQDLLMRIFQDLNNYSECLIPIDEGNAVDIKIFPLLRPPTTCVS 249
Query: 232 VEDVPIAVVNLSDIIDVNWDPTMVKIVPYINGINSVAQIAVSARSDVELVQECIKHLMYY 291
+EDVP++ VNL IIDVNWDPTM+ IVPYI+G+NS+A+I+ + SD LV ECI+HL+YY
Sbjct: 250 LEDVPLSSVNLKKIIDVNWDPTMMSIVPYIDGLNSIAKISKLSNSDPGLVIECIRHLIYY 309
Query: 292 NCVILSDIFQFSNIYAPTSEIHNFLTNPAVAKACQLFVQGDSL-KQSLLPFEGRPNNIYP 350
CV LSDIFQFSNIYAP+S I NFLT+P +A CQ +V + K S LP N
Sbjct: 310 KCVTLSDIFQFSNIYAPSSLIRNFLTDPLMASDCQSYVTFPEVSKISNLPL----NKSLG 365
Query: 351 TGRKQASTSTVHSRRRDSN-----DTATTGSTSSVRDTHRNYLXXXXXXXYFRAHASENT 405
+G + + + +V + + S+ D+ TT +S+ R + + L Y S+ +
Sbjct: 366 SGDQDSPSFSVRRKSKSSSIPSNPDSRTTSFSSTSRVSQNSSLNSSFSSIYKDWRQSQTS 425
Query: 406 SLATG---ENQMKRILPTRSCLFDLYKSLSQGKTVKDWYKQNFEIIRDNRIDVRKLIMFG 462
++ N R LPTRSCLFDLY+SLSQG+T+K WY+ + I+++N ID+R+ I FG
Sbjct: 426 CSSSNIHVINNRNRFLPTRSCLFDLYRSLSQGQTLKTWYESKYMILKENNIDIRRFITFG 485
Query: 463 ITRRLIYRVNSYPIVKNVGTWDILKSLVSKEPKTHYGSGSSKNIFSAADIKVVDTKEEGN 522
+ +R+IYR S+P++ N G S+EPK ++ + TK+ N
Sbjct: 486 LEKRIIYRCYSFPVMINAG---------SREPK---------------EMTPIITKDLVN 521
Query: 523 QSDKLDAADEXXXXXXXXXXXXXDVPNTMSKFARYYDDSPSNNAFTEXXXXXXXXXXXXX 582
L+ + N + PS +F
Sbjct: 522 NDKLLEKRNHNHLLSATGSRNTAQSGNLK-------PERPSKVSFEMQRVS--------- 565
Query: 583 XXFDFGTAGQQKTVTEVPDLGDGXXXXXXXXXXXXXLILLESLSNAETFDKVCTKLKKSR 642
A + T+ ++ D +G IL ES+ NAETFDK+C L K +
Sbjct: 566 -----SLATGKSTMPKLSDEEEG--------------ILEESIRNAETFDKICVLLSKPK 606
Query: 643 HEVELLLKDLGHYNIVNS 660
EVE L +LG + ++NS
Sbjct: 607 LEVESYLNELGEFKVINS 624
>SPAC23H3.03c [P] KOG3789 Nitrogen permease regulator NLRG/NPR2
Length = 409
Score = 173 bits (439), Expect = 7e-43
Identities = 107/335 (31%), Positives = 163/335 (47%), Gaps = 62/335 (18%)
Query: 6 DGFVP-IHTVFYTLFHPTEGSKVRFQFPPNSLEN---GK---IDFDTIKNYIIPKPQLCN 58
D F P + +F+ LF P +G V + P S+ N GK + F+TI +Y+IPK +LCN
Sbjct: 13 DSFPPRLLAIFFALFDPLQGPIVACEAPAGSVTNVDGGKNCLLPFETISDYVIPKRELCN 72
Query: 59 KLLTFKYGEYRIVCYPVNVNARYYARNSXXXXXXXXXPYDCATSPYEPAIARLGKMFKVL 118
K +T Y+++ +P+++ Y RN+ + ++ Y P + RL + +VL
Sbjct: 73 KTITVCTNHYQVIGHPISIIGSNYERNALIFNMCMIFHEEEDSACYIPLVKRLARNLEVL 132
Query: 119 EEQSQILSKSEQNPVYFQLKSNDSNAADQSTEMGSITISEHTDIKSTGDDGEKYLEIIKE 178
E+Q +S + PV F
Sbjct: 133 EKQIHYISDLNKRPVIFS------------------------------------------ 150
Query: 179 LAKNQKSLIIEDLLMKLFLDLNNYSECLIPIDEGNSIDIKLFPVRTPPSSNISVEDVPIA 238
+IE +L D+NN+ EC+I +D+ NSI+IKLFPV P + VPI
Sbjct: 151 --------VIEQILE----DMNNFCECMIQLDDQNSINIKLFPV-FPSPPTVKSFHVPIL 197
Query: 239 VVNLSDIIDVNWDPTMVKIVPYINGINSVAQIAVSARSDVELVQECIKHLMYYNCVILSD 298
L ++D NWD T+ K+ P+INGINSV +IA A Q+C++H +YY C+I++D
Sbjct: 198 TAQLDLLMDKNWDMTVQKVYPFINGINSVQRIAELANVSYRSCQKCMEHFLYYGCLIIAD 257
Query: 299 IFQFSNIYAPTSEIHNFLTNPAVAKACQLFVQGDS 333
IFQF NIYA T+ N L +P + C +V +S
Sbjct: 258 IFQFHNIYAMTTNAPNLLQDPDFQRECTAYVSTNS 292
Score = 57.4 bits (137), Expect = 7e-08
Identities = 31/72 (43%), Positives = 45/72 (62%), Gaps = 4/72 (5%)
Query: 407 LATGENQMKRILPTRSCLFDLYKSLSQGKTVKDWYKQNFEIIRDNRIDVRKLIMFGITRR 466
++T + K + T + +F LY SL QG VKDW +N EI + +DVR+LI FG +
Sbjct: 288 VSTNSSNAKNV--TFATIFKLYCSLRQGLRVKDWMNENKEIFKG--LDVRRLISFGTIKG 343
Query: 467 LIYRVNSYPIVK 478
LIYRV+ YP ++
Sbjct: 344 LIYRVHKYPYLE 355
>Hs5729949 [P] KOG3789 Nitrogen permease regulator NLRG/NPR2
Length = 380
Score = 132 bits (333), Expect = 1e-30
Identities = 89/330 (26%), Positives = 150/330 (44%), Gaps = 59/330 (17%)
Query: 11 IHTVFYTLFHPTEGSKVRFQFPPNSLENGKIDFDTIKNYIIPKPQLCNKLLTFKYGEYRI 70
I +F++ FHPT G K+ +Q P + + FDT++ YII KP+L NKL+T E ++
Sbjct: 7 IECIFFSEFHPTLGPKITYQVPEDFISREL--FDTVQVYIITKPELQNKLITVTAMEKKL 64
Query: 71 VCYPVNVNARYYARNSXXXXXXXXXPYDCATSPYEPAIARLGKMFKVLEEQSQILSKSEQ 130
+ PV + + Y+RN+ T EP + +L LE +S +S E
Sbjct: 65 IGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVSMEES 124
Query: 131 NPVYFQLKSNDSNAADQSTEMGSITISEHTDIKSTGDDGEKYLEIIKELAKNQKSLIIED 190
QK + I
Sbjct: 125 K---------------------------------------------------QKLVPIMT 133
Query: 191 LLMKLFLDLNNYSECLIPIDEGNSIDIKLFPVRTPPSSNISVE-DVPIAVVNLSDIIDVN 249
+L++ +LN C +PIDE N+I +K+ R P ++ E DVP+ + D +
Sbjct: 134 ILLE---ELNASGRCTLPIDESNTIHLKVIEQRPDPP--VAQEYDVPVFTKDKEDFFNSQ 188
Query: 250 WDPTMVKIVPYINGINSVAQIAVSARSDVELVQECIKHLMYYNCVILSDIFQFSNIYAPT 309
WD T +I+PYI+G + +I+ A ++ LV+ I++L+YY V L I Q+SN+Y PT
Sbjct: 189 WDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQYSNVYCPT 248
Query: 310 SEIHNFLTNPAVAKACQLFVQGDSLKQSLL 339
++ + + + ++ +AC +V K++ L
Sbjct: 249 PKVQDLVDDKSLQEACLSYVTKQGHKRASL 278
>7293296 [P] KOG3789 Nitrogen permease regulator NLRG/NPR2
Length = 412
Score = 109 bits (272), Expect = 2e-23
Identities = 88/320 (27%), Positives = 139/320 (42%), Gaps = 60/320 (18%)
Query: 11 IHTVFYTLFHPTEGSKVRFQFPPNSLENGKIDFDTIKNYIIPKPQLCNKLLTFKYGEYRI 70
I +F + FH T G K+ Q P N + K FD I YIIPK L +LT + +I
Sbjct: 40 IRCIFLSEFHATAGCKISCQVPDNYIS--KDVFDAINVYIIPKQHLQRCILTVNAMDVKI 97
Query: 71 VCYPVNV-NARYYARNSXXXXXXXXXPYDCATSPYEPAIARLGKMFKVLEEQSQILSKSE 129
V YPV + + + YARN+ + YEP + +L +
Sbjct: 98 VGYPVGIQDQQKYARNAFLFNLCFVCDSRARSVQYEPVVKKLSEYL-------------- 143
Query: 130 QNPVYFQLKSNDSNAADQSTEMGSITISEHTDIKSTGDDGEKYLEIIKELAKNQKSLIIE 189
I + E + S DD + I + + +
Sbjct: 144 ------------------------IMMEEESCFLSREDDKRRLQNIFETVLR-------- 171
Query: 190 DLLMKLFLDLNNYSECLIPIDEGNSIDIKLFPVRTPPSSNISVEDVPIAVVNLSDIIDVN 249
DLN I ++ N+I +K+ + P + VP+ + NL D N
Sbjct: 172 --------DLNERKVATI-VEGDNTIYLKIV-MHKPDPPPVKDHMVPLLLANLRDAPLDN 221
Query: 250 WDPTMVKIVPYINGINSVAQIAVSARSDVELVQECIKHLMYYNCVILSDIFQFSNIYAPT 309
WD T +I+PYINGIN VA+IA A + +LV+ CI++L+YY V L I ++SN+Y T
Sbjct: 222 WDLTTQQILPYINGINHVARIAAEADVETDLVKSCIQNLVYYGVVQLLPILKYSNVYM-T 280
Query: 310 SEIHNFLTNPAVAKACQLFV 329
+ + + + +++ AC+ +V
Sbjct: 281 QNLKHLIQSASLSGACRKYV 300
>CE28406_2 [P] KOG3789 Nitrogen permease regulator NLRG/NPR2
Length = 427
Score = 90.1 bits (222), Expect = 1e-17
Identities = 83/339 (24%), Positives = 137/339 (39%), Gaps = 69/339 (20%)
Query: 4 TFDGFVPIHTVFYTLFHPTEGSKVRFQFPPNSLENGKID--FDTIKNYIIPKPQLCNKL- 60
TFD + + FH T G+ ++ P EN K++ F K IIPKP +
Sbjct: 24 TFDDAPQLLGILLAEFHNTLGAIIKHSVPK---ENNKVEATFHFTKGLIIPKPSMFRNSF 80
Query: 61 -LTFKYGEYRIVCYPVNVNARYYARNSXXXXXXXXXPY-DCATSPYEPAIARLGKMFKVL 118
LT K +++ +PV ++ + Y R A + YEP + + + L
Sbjct: 81 SLTIKSLRCKVLMFPVGIDHKSYERGRFTFDMAFIVDMTSSAETMYEPIVQKCAEYLIEL 140
Query: 119 EEQSQILSKSEQNPVYFQLKSNDSNAADQSTEMGSITISEHTDIKSTGDDGEKYLEIIKE 178
E + + L+ NP Y
Sbjct: 141 EMEYKFLT----NPKY-------------------------------------------- 152
Query: 179 LAKNQKSLIIEDLLMKLFLDLNNYSECLIPIDEGN----SIDIKLFPVRT---PPSSNIS 231
K+Q + D++ ++F DL+N E +I + + ++ KL P+ PP +
Sbjct: 153 --KDQ----VLDVMERMFNDLSNKGESVIEVSLEDEKIVTLYFKLCPLYRGCEPPEIDYH 206
Query: 232 VEDVPIAVVNLSDIIDVNWDPTMVKIVPYINGINSVAQIAVSARSDVELVQECIKHLMYY 291
+ + I V L+D + D KI+P I+GIN+V +IA+ D LV C+++L +Y
Sbjct: 207 MVPMFIREVTLTDHLIEKMDVLSQKIIPQIDGINTVREIAIILELDPSLVARCVRNLHFY 266
Query: 292 NCVILSDIFQFSNIYAPTSEIHNFLTNPAVAKACQLFVQ 330
CV L +F + N Y T +H+F N C FV+
Sbjct: 267 ECVSLVPMFLYYNTYVATERVHDFYKNQTEINDCLEFVK 305
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.134 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,168,104
Number of Sequences: 60738
Number of extensions: 1451666
Number of successful extensions: 3773
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3748
Number of HSP's gapped (non-prelim): 15
length of query: 660
length of database: 30,389,216
effective HSP length: 113
effective length of query: 547
effective length of database: 23,525,822
effective search space: 12868624634
effective search space used: 12868624634
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)