ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV0384 good M KOG3332 Cell wall/membrane/envelope biogenesis N-acetylglucosaminyl phosphatidylinositol de-N-acetylase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV0384 123160 122372 -263
(263 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR281w [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol de... 171 1e-42
SPAPB2B4.01c [M] KOG3332 N-acetylglucosaminyl phosphatidylinosit... 97 3e-20
ECU08g0300 [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol... 84 2e-16
Hs4758922 [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol ... 80 3e-15
CE19222 [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol de... 65 1e-10
At2g27340 [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol ... 60 4e-09
At3g58130 [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol ... 58 1e-08
>YMR281w [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol
de-N-acetylase
Length = 304
Score = 171 bits (432), Expect = 1e-42
Identities = 96/242 (39%), Positives = 138/242 (56%), Gaps = 34/242 (14%)
Query: 51 VTLVVAHPDDEVMFFSPTLTQFNELLPISIPINVVCMTAGDADGLGHIRKQELVDSLRIM 110
+ LV+AHPDDEVMFFSP ++Q N P ++P N++C++ G+A+GLG R +EL +S ++
Sbjct: 57 INLVIAHPDDEVMFFSPIISQLNSYFPRTVPFNIICLSKGNAEGLGETRVRELNESAALL 116
Query: 111 FHG-RQFGCDVLDFEDGMDAVWDQVLLEKQLRSSIP----DSNPLVLTFDQFGVSGHINH 165
H R V+DF+DGMD +WD + L I + N +++TFD +GVS HINH
Sbjct: 117 LHNERAVSVQVMDFQDGMDEIWDIDSITSSLSQKIDIKNHNLNQIIVTFDSYGVSNHINH 176
Query: 166 ISCGRLVEKL---------------PYSHKLHLRS-DQPIYVKYSAFIAGI--------- 200
SC V+KL P+ L+LRS I +KY++FI I
Sbjct: 177 KSCYAAVKKLVDDYAQPKTKRNEQPPHVTALYLRSYKNNIVLKYNSFIWEILKILYDLIS 236
Query: 201 -FQLGISTVYPDYGKPR---CFISTLPQYLLAASAMSLAHTSQMVWFRVGWWLFSRFCFI 256
F+ I + P+ + ++T QY+LA + M AH SQ+VWFR GWW+FSRF F+
Sbjct: 237 PFRRIIQALPPNTAAEKDKLSLMNTHAQYVLAFATMLNAHESQVVWFRYGWWIFSRFVFV 296
Query: 257 NE 258
NE
Sbjct: 297 NE 298
>SPAPB2B4.01c [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol
de-N-acetylase
Length = 248
Score = 96.7 bits (239), Expect = 3e-20
Identities = 75/219 (34%), Positives = 106/219 (48%), Gaps = 16/219 (7%)
Query: 50 SVTLVVAHPDDEVMFFSPTLTQFNELLPISIPINVVCMTAGDADGLGHIRKQELVDSLRI 109
S+ V AHPDDE MFF PT+ S ++V+C++ G+ADGLG +R++ELV +
Sbjct: 33 SILFVFAHPDDESMFFGPTIDYLGN--QHSTRVHVLCLSNGNADGLGSVREKELVVAASK 90
Query: 110 MFHGRQFGCDVLD--FEDGMDAVWDQVLLEKQLRSSIPDSN-PLVLTFDQFGVSGHINHI 166
+ V D +DGM A WD + K + I N ++TFD G+SGH NHI
Sbjct: 91 YQIDKTNVHVVSDPQLQDGMQAKWDPTDVAKHISQIIERYNIKTLITFDNKGISGHPNHI 150
Query: 167 SC----GRLVEKLPYSHKLHLRSDQPIYVKYSAFIAGIFQLGISTVYPDYGKPRCFI--S 220
+C ++V+ P L S I+ KY +++ I L V G+ I +
Sbjct: 151 ACYEGAMKIVKATPQVQVFVLESVN-IFRKYISYLDTIPTL----VQSQAGRNDTIIIHA 205
Query: 221 TLPQYLLAASAMSLAHTSQMVWFRVGWWLFSRFCFINEL 259
AM H SQMVWFR GW S++ N L
Sbjct: 206 DRKSTQRIRDAMVRGHKSQMVWFRYGWIYLSKYMSNNVL 244
>ECU08g0300 [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol
de-N-acetylase
Length = 246
Score = 84.3 bits (207), Expect = 2e-16
Identities = 75/221 (33%), Positives = 107/221 (47%), Gaps = 53/221 (23%)
Query: 53 LVVAHPDDEVMFFSPTLTQFNELLPISIPINVVCMTAGDADGLGHIRKQELVDSLRIMFH 112
L++AHPDDE MFF+P+L L + I+++C++ G+ +G G R++EL
Sbjct: 59 LLIAHPDDESMFFAPSL------LSLGGDIDILCLSNGNKEGKGKEREKEL--------- 103
Query: 113 GRQFGCDV-------LDFEDGMDAVWDQVLLE-KQLRSSIPDSNPLVLTFDQFGVSGHIN 164
R+ GC F DG D WD V++ K L I +++TFD+ GVSGH N
Sbjct: 104 -RKVGCYTGAKVLITTFFSDGED--WDPVMIYIKLLAIYILRPFDVLMTFDEAGVSGHKN 160
Query: 165 HISCGR---LVEKLPYSHKLHLRSDQPIYVKYSAFIAGIFQLGISTV----YPDYGKPRC 217
HISC + L K+ +S K L + ++ KY ++ F STV Y + K C
Sbjct: 161 HISCSKGAGLFLKI-HSVKGLLLKSKNLFQKYGVDMS--FSRISSTVPFSMYMEPVKMMC 217
Query: 218 FISTLPQYLLAASAMSLAHTSQMVWFRVGWWLFSRFCFINE 258
F H SQMVWFR + LFS F N+
Sbjct: 218 F-----------------HRSQMVWFRYLYVLFSNFMSYND 241
>Hs4758922 [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol
de-N-acetylase
Length = 252
Score = 80.1 bits (196), Expect = 3e-15
Identities = 72/227 (31%), Positives = 102/227 (44%), Gaps = 42/227 (18%)
Query: 53 LVVAHPDDEVMFFSPTLTQFNELLPISIPINVVCMTAGDADGLGHIRKQELVDSLRIMFH 112
LV+AHPDDE MFF+PT+ L + + ++C +AG+ G RK+EL+ S
Sbjct: 45 LVIAHPDDEAMFFAPTVL---GLARLRHWVYLLCFSAGNYYNQGETRKKELLQS------ 95
Query: 113 GRQFGCDVL-------------DFEDGMDAVWDQVLLEKQLRSSIP-DSNPLVLTFDQFG 158
CDVL DF D WD + + L I + LV+TFD G
Sbjct: 96 -----CDVLGIPLSSVMIIDNRDFPDDPGMQWDTEHVARVLLQHIEVNGINLVVTFDAGG 150
Query: 159 VSGHINHISCGRLVE------KLPYSHKLHLRSDQPIYVKYSAFIAGIFQLGISTVYPDY 212
VSGH NHI+ V KLP + + KY + L +S ++
Sbjct: 151 VSGHSNHIALYAAVRALHSEGKLPKGCSVLTLQSVNVLRKY----ISLLDLPLSLLHT-- 204
Query: 213 GKPRCFISTLPQYLLAASAMSLAHTSQMVWFRVGWWLFSRFCFINEL 259
+ F+ + A AMS H SQ++WFR + +FSR+ IN L
Sbjct: 205 -QDVLFVLNSKEVAQAKKAMS-CHRSQLLWFRRLYIIFSRYMRINSL 249
>CE19222 [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol
de-N-acetylase
Length = 151
Score = 64.7 bits (156), Expect = 1e-10
Identities = 43/118 (36%), Positives = 66/118 (55%), Gaps = 8/118 (6%)
Query: 51 VTLVVAHPDDEVMFFSPTLTQFNELLPISIPINVVCMTAGDADGLGHIRKQELVDSL-RI 109
+ L++AHPDDE MFFSPT+ LL + V+C++ G+ DGLG IR +EL + ++
Sbjct: 34 ILLLIAHPDDETMFFSPTI---RALLQAGHRVFVLCISNGNFDGLGKIRARELSRAASKL 90
Query: 110 MFHGRQFGCDVLDFEDGMDA-VWDQ-VLLEKQLRSSIPDSNPLVLTFDQFGVSGHINH 165
C LD+++ D W++ L + +R + V++FD GVSGH NH
Sbjct: 91 GISASDVIC--LDYDEFADGDTWNRNALCQIVMRHVEVLAADTVISFDSHGVSGHHNH 146
>At2g27340 [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol
de-N-acetylase
Length = 223
Score = 59.7 bits (143), Expect = 4e-09
Identities = 57/210 (27%), Positives = 92/210 (43%), Gaps = 41/210 (19%)
Query: 60 DEVMFFSPTLTQFNELLPISIPINVVCMTAGDADGLGHIRKQELVDSLRIMFHGRQFGCD 119
D+ FFSPT+ F + ++++C + G+ADG+G IR QEL + ++
Sbjct: 34 DDGKFFSPTINYFTST---ACNLHILCFSTGNADGMGSIRDQELHRACAVL--------K 82
Query: 120 VLDFEDGMDAVWDQVLLEKQLRSSIPDSNPLVLTFDQFGVSGHINH----------ISCG 169
V+ F+ + + D S + ++TFD +GV GH NH I
Sbjct: 83 VIPFD--KEGICD--------NDSCHCNEEHIITFDNYGVWGHCNHRDVHPPIDCKIDSA 132
Query: 170 RLVEKLPYSHKLHLRSDQPIYVKYSAFIAGIFQLGISTVYPDYGKPRCFISTLPQYLLAA 229
+ + Y H++ L I+ KY G + +S + P I Q +
Sbjct: 133 KRIHGFLYVHQVSLN----IFRKY----CGPVDIWLS-ILSAKRHPSKVIIINKQPWKSF 183
Query: 230 SAMSLAHTSQMVWFRVGWWLFSRFCFINEL 259
AM+ H SQ VWFR + LFS + ++N L
Sbjct: 184 KAMA-QHLSQWVWFRKLFVLFSSYTYVNTL 212
>At3g58130 [M] KOG3332 N-acetylglucosaminyl phosphatidylinositol
de-N-acetylase
Length = 124
Score = 58.2 bits (139), Expect = 1e-08
Identities = 35/113 (30%), Positives = 58/113 (50%), Gaps = 22/113 (19%)
Query: 64 FFSPTLTQFNELLPISIPINVVCMTAGDADGLGHIRKQEL----------VDSLRIMFHG 113
FFSPT+ N L + ++++C++ G+ADG+G IR EL + L+I+ H
Sbjct: 7 FFSPTI---NYLASNACNLHMLCLSTGNADGMGSIRNNELHRACAVLKVPLQQLKILNHP 63
Query: 114 RQFGCDVLDFEDGMDAVWDQVLLEKQLRSSIPDSN-PLVLTFDQFGVSGHINH 165
+ +DG +W LL + + + + ++TFD +GVSGH NH
Sbjct: 64 --------NLQDGFGQLWSHDLLTEIIEEEVTKHDIHTIITFDNYGVSGHCNH 108
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.328 0.142 0.450
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,933,081
Number of Sequences: 60738
Number of extensions: 653990
Number of successful extensions: 1854
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1836
Number of HSP's gapped (non-prelim): 7
length of query: 263
length of database: 30,389,216
effective HSP length: 104
effective length of query: 159
effective length of database: 24,072,464
effective search space: 3827521776
effective search space used: 3827521776
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)