ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV0489 good H KOG1540 Coenzyme transport and metabolism Ubiquinone biosynthesis methyltransferase COQ5
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV0489 154445 155356 304
(304 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YML110c [H] KOG1540 Ubiquinone biosynthesis methyltransferase COQ5 450 e-126
SPCC4G3.04c [H] KOG1540 Ubiquinone biosynthesis methyltransferas... 274 1e-73
7292846 [H] KOG1540 Ubiquinone biosynthesis methyltransferase COQ5 221 1e-57
Hs14150080 [H] KOG1540 Ubiquinone biosynthesis methyltransferase... 220 2e-57
At5g57300 [H] KOG1540 Ubiquinone biosynthesis methyltransferase ... 218 9e-57
CE29014 [H] KOG1540 Ubiquinone biosynthesis methyltransferase COQ5 208 9e-54
At1g23360 [H] KOG1540 Ubiquinone biosynthesis methyltransferase ... 87 4e-17
>YML110c [H] KOG1540 Ubiquinone biosynthesis methyltransferase COQ5
Length = 307
Score = 450 bits (1157), Expect = e-126
Identities = 220/286 (76%), Positives = 249/286 (86%), Gaps = 5/286 (1%)
Query: 23 RSFSNARVQLNQKQSNSDGFSG-----DSTTHFGSRTVLKEEKEKLVGNVFSSVASKYDV 77
R F+ A +++ NS S THFGS+TVLK K+KLVG+VFSSVA++YD+
Sbjct: 21 RCFTQAHRACKEEEVNSPLSSAAEQPEQKYTHFGSKTVLKSTKQKLVGDVFSSVANRYDL 80
Query: 78 MNDVMSLGIHRLWKDHFINKLDAGKRPNSTEPLHFIDVAGGSGDIAFGLLDHAEQKFHDV 137
MNDVMSLGIHRLWKDHFINKLDAGKRPNST PL+FIDVAGGSGDIAFGLLDHAE KF D
Sbjct: 81 MNDVMSLGIHRLWKDHFINKLDAGKRPNSTTPLNFIDVAGGSGDIAFGLLDHAESKFGDT 140
Query: 138 ESTMHIVDINADMLKEGEKRAMDQGKYYNDPRVTFLVQNGETLDQIESNSKDIYTISFGI 197
ESTM IVDIN DMLKEGEKRAM+QGKY+ DPRV FLV NGE L++I+S+SKDIYT+SFGI
Sbjct: 141 ESTMDIVDINPDMLKEGEKRAMEQGKYFKDPRVRFLVSNGEKLEEIDSDSKDIYTVSFGI 200
Query: 198 RNFTDIQAGLNTAYRVLKPGGIFFCLEFSKIDNPLVNLIYQRWAQVLPVMGSVVANDYDS 257
RNFTDIQ GLNTAYRVLKPGGIF+CLEFSKI+NPL++ YQ+WA+VLPVMGS++ANDYDS
Sbjct: 201 RNFTDIQKGLNTAYRVLKPGGIFYCLEFSKIENPLMDFAYQQWAKVLPVMGSMIANDYDS 260
Query: 258 YQYLVESIERFPSQEHFKSMIEKAGFQSAGYENLSFGICAIHWGIK 303
YQYLVESIERFP QE FKSMIEKAGF+SAGYE+L+FGICAIHWGIK
Sbjct: 261 YQYLVESIERFPDQETFKSMIEKAGFKSAGYESLTFGICAIHWGIK 306
>SPCC4G3.04c [H] KOG1540 Ubiquinone biosynthesis methyltransferase COQ5
Length = 305
Score = 274 bits (700), Expect = 1e-73
Identities = 159/311 (51%), Positives = 202/311 (64%), Gaps = 15/311 (4%)
Query: 1 MSMISARARLSLAQSLRINGGLRSFSNARVQLNQ----KQSNSDGFSGDSTTHFGSRTVL 56
MS + A LA L+ G+RS + A +L+ S+ D + THFG + V
Sbjct: 1 MSRLRAPVAKFLADGLK---GIRSTALAGSRLSNCRYTSTSSKDTDTSSHMTHFGFKDVP 57
Query: 57 KEEKEKLVGNVFSSVASKYDVMNDVMSLGIHRLWKDHFINKLDAGKRPNSTEPLHFIDVA 116
++EKE LV NVFSSVA KYD MND MSLGIHRLWK+ F+++L+ G NST P+ +DVA
Sbjct: 58 EDEKEHLVKNVFSSVAKKYDEMNDAMSLGIHRLWKNIFVSRLNPG---NSTVPMKILDVA 114
Query: 117 GGSGDIAFGLLDHAEQKFHDVESTMHIVDINADMLKEGEKRAMDQGKYYNDPRVTFLVQN 176
GG+GDIAF +L+HA D + + + DIN DML G +R+ + YY+ RV F+ QN
Sbjct: 115 GGTGDIAFRILNHATNHNGDRNTRVIVADINPDMLSVGLRRSK-KTPYYDSGRVEFIEQN 173
Query: 177 GETLDQIESNSKDIYTISFGIRNFTDIQAGLNTAYRVLKPGGIFFCLEFSKI-DNPLVNL 235
E LD+I NS D+YTI+FGIRN T I L AYRVLKPGG+F CLEFSK+ PL L
Sbjct: 174 AEILDKIPDNSIDMYTIAFGIRNCTHIPKVLEQAYRVLKPGGVFSCLEFSKVYPAPLAEL 233
Query: 236 IYQRWAQVLPVMGSVVANDYDSYQYLVESIERFPSQEHFKSMIEKAGFQSA---GYENLS 292
Q ++LP++G+++A D SY+YLVESIERFP + F MIE AGF A GYE LS
Sbjct: 234 YRQYSFKILPLLGTIIAGDSQSYEYLVESIERFPDAKTFAKMIEDAGFTLAGETGYETLS 293
Query: 293 FGICAIHWGIK 303
FGI AIH GIK
Sbjct: 294 FGIAAIHTGIK 304
>7292846 [H] KOG1540 Ubiquinone biosynthesis methyltransferase COQ5
Length = 301
Score = 221 bits (562), Expect = 1e-57
Identities = 129/307 (42%), Positives = 178/307 (57%), Gaps = 20/307 (6%)
Query: 10 LSLAQSLRINGGLRSFSNARVQLNQKQSNSDGFSG-------DSTTHFGSRTVLKEEKEK 62
+ +S R+ R F + R Q++ SG + TTHFG +TV + EKE+
Sbjct: 1 MQTTRSTRLLSLARRFVHTRTASQSAQNSKGMASGAESISGKEKTTHFGFQTVRESEKEQ 60
Query: 63 LVGNVFSSVASKYDVMNDVMSLGIHRLWKDHFINKLDAGKRPNSTEPLHFIDVAGGSGDI 122
V VF VA+ YDVMND MSLGIHR+WKD F+ +L T + +D+AGG+GDI
Sbjct: 61 KVHEVFEQVANSYDVMNDAMSLGIHRVWKDVFVERL------GPTHGMRLLDMAGGTGDI 114
Query: 123 AFGLLDHAEQKFHDVESTMHIV--DINADMLKEGEKRAMDQG---KYYNDPRVTFLVQNG 177
F L + + + + H+ DIN ML GE+RA G ++ V + +
Sbjct: 115 TFRYLRYLNNQPNPQQRPSHVTVSDINQHMLNVGEERAKRLGLTTDQLSNCTVAWQCADA 174
Query: 178 ETLDQIESNSKDIYTISFGIRNFTDIQAGLNTAYRVLKPGGIFFCLEFSKIDNPLVNLIY 237
E L ++ S YTI+FGIRN T + L+ AYRVL+PGG F CLEFS + N + +Y
Sbjct: 175 EKLPFPDA-SFTAYTIAFGIRNCTHVDKVLSEAYRVLQPGGRFMCLEFSHLTNETMQWLY 233
Query: 238 QRWA-QVLPVMGSVVANDYDSYQYLVESIERFPSQEHFKSMIEKAGFQSAGYENLSFGIC 296
+++ QV+P MG ++A + +YQYLVESI RFP QE FK MIE+AGF YENL+FG+
Sbjct: 234 DQYSFQVIPPMGQLLAGQWQAYQYLVESIRRFPKQEQFKQMIEQAGFDQVSYENLTFGVV 293
Query: 297 AIHWGIK 303
+IH G K
Sbjct: 294 SIHSGFK 300
>Hs14150080 [H] KOG1540 Ubiquinone biosynthesis methyltransferase COQ5
Length = 307
Score = 220 bits (560), Expect = 2e-57
Identities = 128/285 (44%), Positives = 171/285 (59%), Gaps = 39/285 (13%)
Query: 48 THFGSRTVLKEEKEKLVGNVFSSVASKYDVMNDVMSLGIHRLWKDHFINKLDAGKRPNST 107
THFG TV +EEK V VF SVA KYDVMND+MSLGIHR+WKD + K+ +
Sbjct: 32 THFGFETVSEEEKGGKVYQVFESVAKKYDVMNDMMSLGIHRVWKDLLLWKM------HPL 85
Query: 108 EPLHFIDVAGGSGDIAFGLLDHAEQKFHDVE----------------------------S 139
+DVAGG+GDIAF L++ + + + S
Sbjct: 86 PGTQLLDVAGGTGDIAFRFLNYVQSQHQRKQKRQLRAQQNLSWEEITKEYQNEEDSLGGS 145
Query: 140 TMHIVDINADMLKEGEKRAMDQGKYYNDPRVTFLVQNGETLDQIESNSKDIYTISFGIRN 199
+ + DIN +MLK G+++A+ QG + +++ + E L + + DIYTI+FGIRN
Sbjct: 146 RVVVCDINKEMLKVGKQKALAQGYRAG---LAWVLGDAEELP-FDDDKFDIYTIAFGIRN 201
Query: 200 FTDIQAGLNTAYRVLKPGGIFFCLEFSKIDNPLVNLIYQRWA-QVLPVMGSVVANDYDSY 258
T I L A+RVLKPGG F CLEFS+++NPL++ +Y ++ QV+PV+G V+A D+ SY
Sbjct: 202 VTHIDQALQEAHRVLKPGGRFLCLEFSQVNNPLISRLYDLYSFQVIPVLGEVIAGDWKSY 261
Query: 259 QYLVESIERFPSQEHFKSMIEKAGFQSAGYENLSFGICAIHWGIK 303
QYLVESI R PSQE FK MIE AGF YE+L+ GI AIH G K
Sbjct: 262 QYLVESIRRSPSQEEFKDMIEDAGFHKVTYESLTSGIVAIHSGFK 306
>At5g57300 [H] KOG1540 Ubiquinone biosynthesis methyltransferase COQ5
Length = 288
Score = 218 bits (555), Expect = 9e-57
Identities = 125/269 (46%), Positives = 167/269 (61%), Gaps = 23/269 (8%)
Query: 48 THFGSRTVLKEEKEKLVGNVFSSVASKYDVMNDVMSLGIHRLWKDHFINKLD--AGKRPN 105
T FG + V +EEK KLVGNVF++VAS YD+MNDVMS G+HRLWK+ + KL AG +
Sbjct: 29 TSFGFQEVKEEEKSKLVGNVFTNVASSYDIMNDVMSGGLHRLWKERLVGKLSPFAGMK-- 86
Query: 106 STEPLHFIDVAGGSGDIAFGLLD-------HAEQKFHDV---ESTMHIVDINADMLKEGE 155
+DVAGG+GD+AF + D A QK + E+ +++ DIN +ML G+
Sbjct: 87 ------HLDVAGGTGDVAFRIYDAVYSVKRRALQKVDEASLEETQIYVCDINPNMLNVGK 140
Query: 156 KRAMDQGKYYNDPRVTFLVQNGETLDQIESNSKDIYTISFGIRNFTDIQAGLNTAYRVLK 215
+RA ++G D + V+ + NS D YTI+FGIRN T I+ L AYRVLK
Sbjct: 141 QRAAERG--LRDNKSLVWVEGDAEALSFDDNSMDGYTIAFGIRNVTHIEKALAEAYRVLK 198
Query: 216 PGGIFFCLEFSKIDNPLVNLIYQRWA-QVLPVMGSVVANDYDSYQYLVESIERFPSQEHF 274
GG F CLE S ++ P+ +Y ++ QV+P +G ++A D +SYQYLVES+ RFP QE F
Sbjct: 199 RGGRFLCLELSHVEIPVFKNLYDLYSFQVIPNLGELIAGDRESYQYLVESVRRFPPQERF 258
Query: 275 KSMIEKAGFQSAGYENLSFGICAIHWGIK 303
SMI AGF+ YENL G+ AIH IK
Sbjct: 259 ASMIADAGFEKVEYENLVGGVVAIHSAIK 287
>CE29014 [H] KOG1540 Ubiquinone biosynthesis methyltransferase COQ5
Length = 285
Score = 208 bits (529), Expect = 9e-54
Identities = 119/284 (41%), Positives = 176/284 (61%), Gaps = 19/284 (6%)
Query: 20 GGLRSFSNARVQLNQKQSNSDGFSGDSTTHFGSRTVLKEEKEKLVGNVFSSVASKYDVMN 79
G R FS +NQ S++ THFG V + EKE+ V +VF++VA KYD+MN
Sbjct: 18 GNFRQFS-----VNQVNSDNKRSEPGKKTHFGFTDVDEAEKEQKVHHVFANVAKKYDLMN 72
Query: 80 DVMSLGIHRLWKDHFINKLDAGKRPNSTEPLHFIDVAGGSGDIAFGLLDHAEQKFHDVES 139
D MS+G+HRLWKD+++ L P + + L D+AGG+GDIAF +L H+ +
Sbjct: 73 DAMSMGVHRLWKDYYVGGLQV---PYNAKCL---DMAGGTGDIAFRILRHSPT------A 120
Query: 140 TMHIVDINADMLKEGEKRAMDQGKYYNDPRVTFLVQNGETLDQIESNSKDIYTISFGIRN 199
+ + DIN ML G+KRA ++ + R ++ N E + ESN+ D++T+SFGIRN
Sbjct: 121 KVTVSDINQPMLDVGKKRA-EKERDIQPSRAEWVCANAEQMP-FESNTYDLFTMSFGIRN 178
Query: 200 FTDIQAGLNTAYRVLKPGGIFFCLEFSKIDNPLVNLIYQRWAQVLPVMGSVVANDYDSYQ 259
T + + A+RVLKPGG LEFS++++ L + V+PV+G ++A+D SYQ
Sbjct: 179 CTHPEKVVREAFRVLKPGGQLAILEFSEVNSALKPIYDAYSFNVIPVLGEILASDRASYQ 238
Query: 260 YLVESIERFPSQEHFKSMIEKAGFQSAGYENLSFGICAIHWGIK 303
YLVESI +FP+Q+ F +I + GF + YENL+FG+C+IH G+K
Sbjct: 239 YLVESIRKFPNQDEFARIIREEGFSNVRYENLTFGVCSIHKGMK 282
>At1g23360 [H] KOG1540 Ubiquinone biosynthesis methyltransferase COQ5
Length = 281
Score = 86.7 bits (213), Expect = 4e-17
Identities = 73/246 (29%), Positives = 120/246 (48%), Gaps = 26/246 (10%)
Query: 53 RTVLKEEKEKLVGNVFSSVASKYDVMNDVMSLGIHRLWKDHFINKLDAGKRPNSTEPLHF 112
RTV+K E+ + +F+ +A YD +ND++SLG HR+WK+ ++ A K +
Sbjct: 26 RTVVKCSNERRI--LFNRIAPVYDNLNDLLSLGQHRIWKNMAVSWSGAKKGD------YV 77
Query: 113 IDVAGGSGDIAFGLLDHAEQKFHDVESTMHIVDINADMLKEGEKRAMDQGKYYNDPRVTF 172
+D+ GSGD+AF L + V ST ++ ++ E+ A+ + R +
Sbjct: 78 LDLCCGSGDLAFLLSE-------KVGSTGKVMGLDF----SSEQLAVAATRQSLKARSCY 126
Query: 173 LVQNGETLD-QIESNSKDIYTISFGIRNFTDIQAGLNTAYRVLKPGGIFFCLEFSKIDNP 231
G+ +D + D T+ +G+RN D + YRVLKPG L+F+K N
Sbjct: 127 KWIEGDAIDLPFDDCEFDAVTMGYGLRNVVDRLRAMKEMYRVLKPGSRVSILDFNK-SNQ 185
Query: 232 LVNLIYQRWAQVLPVMGSVVANDYD---SYQYLVESIERFPSQEHFKSMIEKAGFQSAGY 288
V Q W ++ + VA YD Y+YL SI + + E +++ +AGF SA +
Sbjct: 186 SVTTFMQGW--MIDNVVVPVATVYDLAKEYEYLKYSINGYLTGEELETLALEAGFSSACH 243
Query: 289 ENLSFG 294
+S G
Sbjct: 244 YEISGG 249
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.136 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,873,257
Number of Sequences: 60738
Number of extensions: 761943
Number of successful extensions: 2014
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1987
Number of HSP's gapped (non-prelim): 8
length of query: 304
length of database: 30,389,216
effective HSP length: 106
effective length of query: 198
effective length of database: 23,950,988
effective search space: 4742295624
effective search space used: 4742295624
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)