ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV0552 good U KOG3065 Intracellular trafficking, secretion, and vesicular transport SNAP-25 (synaptosome-associated protein) component of SNARE complex
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV0552 175547 173880 -556
(556 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR009c [U] KOG3065 SNAP-25 (synaptosome-associated protein) com... 406 e-113
SPBC26H8.02c [U] KOG3065 SNAP-25 (synaptosome-associated protein... 162 1e-39
YMR017w [U] KOG3065 SNAP-25 (synaptosome-associated protein) com... 122 1e-27
CE17152 [U] KOG3065 SNAP-25 (synaptosome-associated protein) com... 55 2e-07
>YGR009c [U] KOG3065 SNAP-25 (synaptosome-associated protein) component of
SNARE complex
Length = 651
Score = 406 bits (1043), Expect = e-113
Identities = 264/648 (40%), Positives = 341/648 (51%), Gaps = 115/648 (17%)
Query: 14 EATPEENREYLNELGISTKTTPSNRREKFAAYGKFANDKVKSKVYAPEGYEQYANPGAQP 73
EATPE+N++ L ELGIS K R+EKFAAYGKFANDK + KVYAP GYEQYA P
Sbjct: 14 EATPEQNKDTLMELGISVKNPSKKRKEKFAAYGKFANDKAEDKVYAPPGYEQYARP---- 69
Query: 74 SERQQLDDLNNSPYD----EVSMINNRNASAS-----ARHTGNYVDPYS--ND------- 115
+ +L+DLN SP D E + +NR +S + + + DPY+ ND
Sbjct: 70 --QDELEDLNASPLDANANEATAGSNRGSSGTQDLGNGAESNSMQDPYAIENDDYRYDDD 127
Query: 116 ----------------NASPYGN-------------SDSSPYGNASASPYDTGYNA---- 142
NA+PYG+ ++ PY N + S N
Sbjct: 128 PYARFQANKSNGRGSVNAAPYGDYGGGYNGTSLNSYNNDGPYSNQNTSNSWVNANGRNSL 187
Query: 143 ----------PLQQNNNPXXXXXXXXXXXXXXXXXXX---------XDTYXXXXXXXXXX 183
P +Q P D
Sbjct: 188 NHSNSTLNVGPSRQTRQPPVSTSTNSLSLDQRSPLANPMQEKRNPYADMNSYGGAYDSNT 247
Query: 184 XXXGATGQRPS---NPYANM-----------NSQDPYGTNSYSNSGSVNAYKGFTPNVAA 229
T Q S NPYA+M S +PY + S S A +TP+ A
Sbjct: 248 NRSSGTRQGSSKNANPYASMANDSYSNGNLNRSANPYSSRSVRQPQSQQAPMTYTPSFIA 307
Query: 230 SDVSSRSYASPRHSTSATRSNDREFNFERSGAD---------------------DEIDLN 268
SD ++R+ + T EF+FE AD D IDLN
Sbjct: 308 SDEAARNSEVDLNEEPRTG----EFDFEEVYADKSAENRAALDEPDLNAVMTNEDSIDLN 363
Query: 269 ETIRPEIDNPKGVQRXXXXXXXXXXXSIHENEGIGTPAKGYQTFEDIQKESQRAEQQEED 328
+ + Q+ + +GY+TFE+IQKE + +QQEED
Sbjct: 364 ASEVDHSSRQQQQQQWFMDEQQQQQQHFNATNNQYGDQRGYKTFEEIQKEEEARQQQEED 423
Query: 329 EEVDGIKQQIKFTKQSSVASTRNTLKMAQDAELAGMNTIGMLGHQSEKLNNVERNLNLMQ 388
E VD IKQ+IKFTKQSSVASTRNTLKMAQDAE AGMNT+GMLGHQSE+LNNVE NL+LM+
Sbjct: 424 EAVDEIKQEIKFTKQSSVASTRNTLKMAQDAERAGMNTLGMLGHQSEQLNNVEGNLDLMK 483
Query: 389 IQNRVADDKVSELKKLNRNILAVHVSNPFHSKRRAREAEDKIKLQRMQDKQNQEDITSTL 448
+QN+VAD+KV+ELKKLNR+ILAVHVSNPF+SKRR RE E+++K +++++K +E + L
Sbjct: 484 VQNKVADEKVAELKKLNRSILAVHVSNPFNSKRRRREREEQLKNRKIEEKLMREQTSQQL 543
Query: 449 HQSTKRIENAMSSGHGPSEVRDRYQNQQILEKAKRYQFXXXXXXXXXXXXXXKNLDKIGQ 508
QST+RIE AM++ + SEVR+RYQ + +LEKAKRYQF +NLD+I Q
Sbjct: 544 SQSTQRIEGAMNANNNISEVRERYQRKNVLEKAKRYQFENDEEDDEMELEIDRNLDQIQQ 603
Query: 509 ISGRLKKLAITTGKEIDSQQDRIKRIEESTDDLDIKIHVNTTRLTNIR 556
+S RLKK+A+TTGKE+DSQQ R+ IEESTDDLDI +H+NT RL IR
Sbjct: 604 VSNRLKKMALTTGKELDSQQKRLNNIEESTDDLDINLHMNTNRLAGIR 651
>SPBC26H8.02c [U] KOG3065 SNAP-25 (synaptosome-associated protein) component of
SNARE complex
Length = 419
Score = 162 bits (410), Expect = 1e-39
Identities = 114/377 (30%), Positives = 187/377 (49%), Gaps = 34/377 (9%)
Query: 194 SNPYANMNSQDPYGT----NSYSNSGSVNAY-----------KGFTPNVAASDVSSRSYA 238
SN Y N S + YG+ N+Y ++GS N Y G++ +D +R
Sbjct: 61 SNNYGNYGSSNNYGSYGASNTYGSNGSSNNYGNYGATNSNGDAGYSITPIRNDPYARKDM 120
Query: 239 SPRHSTSATRSNDREFNFERSGADDEIDLNETIRPEIDNPKGVQRXXXXXXXXXXXSIHE 298
P S++A +R + + D +DL + E+ +Q +
Sbjct: 121 PPMKSSAAV--TERPSMHRSAPSQDTLDLK---KQELFAGARIQNDDESTTDTIPHNDDG 175
Query: 299 NEGIGTPAKGYQTFEDIQKESQRAEQQEEDEEVDGIKQQIKFTKQSSVASTRNTLKMAQD 358
EG +GY+ + EED+EV+ IKQ+I+F KQ S++STRN L MA +
Sbjct: 176 TEG-DEYGEGYR------------DGYEEDQEVEAIKQKIQFVKQDSLSSTRNALLMAGN 222
Query: 359 AELAGMNTIGMLGHQSEKLNNVERNLNLMQIQNRVADDKVSELKKLNRNILAVHVSNPFH 418
AE G+ T+ LG Q+EK+ E+ L++ +I + A+++ ELK LNR++ A+HV P+
Sbjct: 223 AEQMGLATLANLGEQTEKIATAEKELDISKIHAKRAEEQARELKTLNRSMFAIHVPKPWG 282
Query: 419 SKRRAREAEDKIKLQRMQDKQNQEDITSTLHQSTKRIENAMSSGHGPSEVRDRYQNQQIL 478
+R E ++ +R ++Q++ ++S KRI+ AM ++ + + IL
Sbjct: 283 KAKRVAAEEARLAAKRDAERQDEMLNRQFAYRSQKRIDQAMKDNMKSNKKKGDSKGVSIL 342
Query: 479 EKAKRYQFXXXXXXXXXXXXXXKNLDKIGQISGRLKKLAITTGKEIDSQQDRIKRIEEST 538
E++ YQF NLD+IG ++ RLK LA TG+EIDSQ R+ I + +
Sbjct: 343 ERS-HYQFEPDAEDDAMEKEIDGNLDQIGALATRLKGLAYATGQEIDSQNARLGSIHDKS 401
Query: 539 DDLDIKIHVNTTRLTNI 555
D LD +++N RL +I
Sbjct: 402 DRLDTDVYLNVERLRHI 418
>YMR017w [U] KOG3065 SNAP-25 (synaptosome-associated protein) component of
SNARE complex
Length = 397
Score = 122 bits (306), Expect = 1e-27
Identities = 79/261 (30%), Positives = 129/261 (49%), Gaps = 4/261 (1%)
Query: 296 IHENEGIGTPAKGYQTFEDIQKESQRAE-QQEEDEEVDGIKQQIKFTKQSSVASTRNTLK 354
IH + G Y + ++ + Q EEDE VD ++ +I+ TK SV +T TL+
Sbjct: 135 IHRDLGNFANENDYPQWRKVESQYNLENVQPEEDEIVDRLRSEIRSTKLKSVKTTSRTLE 194
Query: 355 MAQDAELAGMNTIGMLGHQSEKLNNVERNLNLMQIQNRVADDKVSELKKLNRNILAVHVS 414
A +A G + L QS +L +E N ++++IQ+ VAD K+ EL NR++LA+
Sbjct: 195 KAIEARCTGKRVLQQLSCQSNQLTKIESNCDMLKIQSNVADRKIDELAHENRSLLALKSP 254
Query: 415 NPFHSKRRAREAEDKIKLQRMQDKQNQEDITSTLHQSTKRIENAMSSGHGPSEVRDRYQN 474
NPF K+R RE D+I +++ + Q++ S K + +SS +G +
Sbjct: 255 NPFR-KKREREKRDQIYNLKLKHRHLQQETMKRAQDSDKNLAINLSSEYG--RYGQGVER 311
Query: 475 QQILEKAKRYQFXXXXXXXXXXXXXXKNLDKIGQISGRLKKLAITTGKEIDSQQDRIKRI 534
Q+IL A++YQF NL++I +SG LK +A G+E ++Q R+ I
Sbjct: 312 QRILRDAQKYQFEADEEDNQMEIDLYGNLEQIKAVSGDLKIMAHAFGREFEAQNTRMFDI 371
Query: 535 EESTDDLDIKIHVNTTRLTNI 555
E + D + RL +
Sbjct: 372 ENNVQQADNALQAKRYRLEKV 392
>CE17152 [U] KOG3065 SNAP-25 (synaptosome-associated protein) component of
SNARE complex
Length = 277
Score = 55.5 bits (132), Expect = 2e-07
Identities = 51/217 (23%), Positives = 92/217 (41%), Gaps = 23/217 (10%)
Query: 328 DEEVDGIKQQIKFTKQSSVASTRNTLKMAQDAELAGMNTIGMLGHQSEKLNNVERNLNLM 387
++E D +++I+ T Q S+ ST + + +++E G +T L Q EKL N E+NL+ +
Sbjct: 33 EDEADYYEREIEKTLQESLDSTERSRRHLENSEKIGTSTAQQLLEQREKLENTEKNLDEI 92
Query: 388 QIQNRVADDKVSELKKLNRNILAVHVSNPFHSKRRAREAEDKIKLQRMQDKQNQEDITST 447
+ +++ + N N L F +K + E Q K S
Sbjct: 93 H--------RTTQMTQRNLNSLKSFFGGMFKNKFTKKPQEPTETPTVPQSKS-----ASR 139
Query: 448 LHQSTKRIENAMSSG--HGPSEVRDRYQNQQILEKAKRYQFXXXXXXXXXXXXXXKNLDK 505
L ++ + + S GPS R ++ + K R++ +NLD
Sbjct: 140 LSETATNLSSGGGSATFSGPSGQRTLTESSRSAIKGTRWE--------AMDNQIDENLDM 191
Query: 506 IGQISGRLKKLAITTGKEIDSQQDRIKRIEESTDDLD 542
+ L++L GKE+DSQ + + RI+ + D
Sbjct: 192 MSANLRNLQRLGADLGKEVDSQNEMLDRIQYKAERND 228
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.306 0.125 0.338
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,747,429
Number of Sequences: 60738
Number of extensions: 1223748
Number of successful extensions: 4458
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4444
Number of HSP's gapped (non-prelim): 7
length of query: 556
length of database: 30,389,216
effective HSP length: 111
effective length of query: 445
effective length of database: 23,647,298
effective search space: 10523047610
effective search space used: 10523047610
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (22.0 bits)