ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIV0586   suspect: LH   S     KOG2913  Function unknown                                               Predicted membrane protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIV0586  186452  187300 283  
         (283 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDR090c [S] KOG2913 Predicted membrane protein                        313   2e-85
SPAC2E12.03c [S] KOG2913 Predicted membrane protein                   108   7e-24

>YDR090c [S] KOG2913 Predicted membrane protein
          Length = 310

 Score =  313 bits (802), Expect = 2e-85
 Identities = 144/223 (64%), Positives = 174/223 (77%), Gaps = 1/223 (0%)

Query: 1   MISEKAAYALATLGTVCWCVQLIPQIIYNYIRKDCTGFPPSMMFLWVVSGVPFGIYFLIT 60
           MISEKAA ALAT+ TVCWCVQLIPQIIYN+ +KDCTG PP MMFLWVVSG+PF IYF ++
Sbjct: 1   MISEKAATALATIATVCWCVQLIPQIIYNWKKKDCTGLPPLMMFLWVVSGIPFAIYFCVS 60

Query: 61  RGNIILQIQPHLFMFFCSLSWVQSLYYPPYSLSVKKILLRCILPMILFIVSFEIGFTLWL 120
           +GN+ILQ+QPHLFMFFCS+S+VQS YYPP S++  KI++  +  +I   V  E+GF LWL
Sbjct: 61  KGNVILQVQPHLFMFFCSISFVQSCYYPPISMARSKIVM-IVAAIIAADVGMEVGFILWL 119

Query: 121 RPVYDHGTHWPXXXXXXXXXXXXXXXXXPPYFELAKRQGRVVGINFIFLFVDSLGAWCSI 180
           RP+Y+ G  WP                 PPYFELAKR+GRV+GINF FLF+DSLGAW SI
Sbjct: 120 RPLYEKGVKWPDLIFGISASVLLAVGLLPPYFELAKRKGRVIGINFAFLFIDSLGAWLSI 179

Query: 181 ASVIVGNMDIMGIVLYAVVAALELGIFASHFIWCCRFKWFGNG 223
            SVI+GNMDIMGI+LY++VA +ELGIFASHFIW CRF++   G
Sbjct: 180 ISVILGNMDIMGIILYSIVAGMELGIFASHFIWWCRFRFLAKG 222


>SPAC2E12.03c [S] KOG2913 Predicted membrane protein
          Length = 283

 Score =  108 bits (271), Expect = 7e-24
 Identities = 64/208 (30%), Positives = 99/208 (46%), Gaps = 3/208 (1%)

Query: 6   AAYALATLGTVCWCVQLIPQIIYNYIRKDCTGFPPSMMFLWVVSGVPFGIYFLITRGNII 65
           A+   A LGTVCWCVQLIPQII NY  K   G     +  WVV+ +P  +Y  +   NI 
Sbjct: 18  ASTVFAILGTVCWCVQLIPQIIKNYRAKSTEGLDTLFILSWVVASIPLSVYNQVQELNIA 77

Query: 66  LQIQPHLFMFFCSLSWVQSLYYPPYSLSVKKILLRCILPMILFIVSFEIGFTLWLRPVYD 125
           L++QP LF      ++ Q LYY      ++K L   ++  +LF    +    L ++    
Sbjct: 78  LKVQPELFQALAFTTFFQCLYYGS-KWPLRKALF-VVISFMLFSGGLQAMLILTIKLGIR 135

Query: 126 HGTHWPXXXXXXXXXXXXXXXXXPPYFELAKRQGRVVGINFIFLFVDSLGAWCSIASVIV 185
               WP                 P Y  + + +  V GI+++FL +DS G+  S  S+  
Sbjct: 136 RHVEWPVVFMGVLATVLVNIGFLPQYISIFRARA-VTGISYLFLAIDSSGSLFSFLSLPF 194

Query: 186 GNMDIMGIVLYAVVAALELGIFASHFIW 213
              D++  V Y ++  +E+G+F   FI+
Sbjct: 195 DRWDVLAAVDYGLLFIIEMGVFVLAFIF 222


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.329    0.146    0.484 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,870,842
Number of Sequences: 60738
Number of extensions: 600915
Number of successful extensions: 1413
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1408
Number of HSP's gapped (non-prelim): 3
length of query: 283
length of database: 30,389,216
effective HSP length: 105
effective length of query: 178
effective length of database: 24,011,726
effective search space: 4274087228
effective search space used: 4274087228
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)