ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV0604 good S KOG3783 Function unknown Uncharacterized conserved protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV0604 190557 192731 725
(725 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKR018c [S] KOG3783 Uncharacterized conserved protein 904 0.0
YJL082w [S] KOG3783 Uncharacterized conserved protein 827 0.0
SPAC11E3.14 [S] KOG3783 Uncharacterized conserved protein 155 2e-37
SPBC83.16c [S] KOG3783 Uncharacterized conserved protein 142 2e-33
>YKR018c [S] KOG3783 Uncharacterized conserved protein
Length = 725
Score = 904 bits (2336), Expect = 0.0
Identities = 462/732 (63%), Positives = 568/732 (77%), Gaps = 14/732 (1%)
Query: 1 MFRVFNALTGAGSPKATELSGEERTKFILQQAHDFEIALQAMDYVLDDNAEVGLKLLADN 60
MF+VF + ELS EE+TK IL+QAHDFE AL+AMDYVLDDNA+ GL LL ++
Sbjct: 1 MFKVFGF---GAKEEIPELSQEEKTKAILKQAHDFEQALRAMDYVLDDNADEGLALLDES 57
Query: 61 QATSPGDATVGALAKGVIEFLEATLGFEPEVMKKASTTLAEAEQLSLKSRAKLQKLNIKT 120
A D T+ ALA+GVIEFLEATLGFE E MKKAS TLA+AE LSLKSR + QK+ +K+
Sbjct: 58 DAKEASDQTINALARGVIEFLEATLGFEAEEMKKASATLAKAEALSLKSRERAQKIGLKS 117
Query: 121 SSLYPPGTEYAVTYTESCLLHALLMLFSESMVEGAKALFKLRKAYHMLQDILKEINASEK 180
SSLYPPGT YAVTYTESCLLHALLM+FSESM+E AKA+ KLRK+Y+MLQ+IL+ I A+ K
Sbjct: 118 SSLYPPGTVYAVTYTESCLLHALLMIFSESMMEAAKAILKLRKSYYMLQEILETIKAANK 177
Query: 181 RKRSSIYL-QEINEST-ASFISSGTCFTSYDIPYKLTPEEEQDKELLDLANKVYSLRRKR 238
K+ I E EST A+FI+ G F S DIPY+LTPEE++DK+LL A +++S+R +R
Sbjct: 178 AKKLKITSGSEDKESTPATFITGGDAFNSVDIPYELTPEEQKDKDLLQFAEQIHSMRTER 237
Query: 239 LCGAHIGNSPAINRLRDDVGAASLKKGSDEETQEFQLLSDNANINQATMDEFIHSGVNLC 298
L GAHIGNSPAINRLR ++G +++ +EE + ++LSD+ +++QAT+DEF+HSGVNLC
Sbjct: 238 LSGAHIGNSPAINRLRGELGLQAMEDLPEEEITDHKVLSDDIDLSQATIDEFVHSGVNLC 297
Query: 299 FGILQVVLSLIPPAIGAVLSVVGFHGSREEGLRLVWKSTKDRNIHXXXXXXXXXFYYDGP 358
FGILQVV+SL+PPAIGAVLSVVGF GSREEGLRLVWK+TK RN+H FYYDGP
Sbjct: 298 FGILQVVISLLPPAIGAVLSVVGFRGSREEGLRLVWKATKQRNVHGCIGLLALMFYYDGP 357
Query: 359 FQFTDIDFDIPAARDDNLPVT-----EMDRPTLLHPGKILTSALLQARALFPNSALWLLQ 413
FQFTD DFDIPAA D+ EMD PTLLHPGKIL ALLQ+RALFPNSALWLL
Sbjct: 358 FQFTDDDFDIPAAVKDSSNSEDSEDEEMDGPTLLHPGKILEDALLQSRALFPNSALWLLN 417
Query: 414 EARMLSKQGRLKEAVDLLDSIDHNTIEMKQVKALIVFEKATTLVYMHEFERGAETMLIML 473
EARMLS +GRL+EAV L+DSID + I M+QVK+L++F++A TL+++H+++R AE +L +L
Sbjct: 418 EARMLSGKGRLEEAVALMDSIDVSKIRMRQVKSLMIFDRAITLIHLHQYDRAAEDILSLL 477
Query: 474 SISEWSHALYTYFAGCCYLEMFRKYEMGILNGENXXXXXXXXXXRATNLVFESANMVGKK 533
IS+WSHA YTYFAGCCYLE +R EMG++ + +A L+F S N++GKK
Sbjct: 478 DISDWSHAFYTYFAGCCYLENWRMCEMGLMKSDKKDEYQK----KAEELIFTSVNLLGKK 533
Query: 534 KFMSKILPLDRFLLRKVDQFKKFQNIIKSSDPLDSIGVSPVHELIYFYNGYNRMTMKELN 593
F SK LPLDRF+LRKV+QFK + + +PLD I SPVHE+ YFYNGYNRM+ + L
Sbjct: 534 TFKSKNLPLDRFILRKVEQFKAKKEELGVENPLDGIATSPVHEIAYFYNGYNRMSEEHLE 593
Query: 594 LSLKSLTEYRNPTIDLQNPDQELIKDLLTSLVLRRTGKIEEGCKILDTQVLPQLFTIQND 653
L+ K LTEYRNP I+ + DQELIKDLL SL LRR G I+EGC ILD +VLP+ F+IQN
Sbjct: 594 LTKKMLTEYRNPAIEALDSDQELIKDLLVSLTLRRLGHIQEGCDILDEKVLPKFFSIQNG 653
Query: 654 KVKYIKKTEDPWVYPTAFYERALFSWKLKGMNGLHEAHEWLIRAQNYQDDYELSTRIGMK 713
KVKYIKKTEDPW YPTA YERALF+WKL+GM+GL E+ EWL+RAQ Y DDYELSTR+GMK
Sbjct: 654 KVKYIKKTEDPWAYPTALYERALFTWKLEGMDGLPESKEWLLRAQGYADDYELSTRVGMK 713
Query: 714 IKAAKDRVEESI 725
IKAA DRV+ S+
Sbjct: 714 IKAAIDRVDHSL 725
>YJL082w [S] KOG3783 Uncharacterized conserved protein
Length = 731
Score = 827 bits (2137), Expect = 0.0
Identities = 430/742 (57%), Positives = 538/742 (71%), Gaps = 29/742 (3%)
Query: 1 MFRVFNALTGAGSPKATELSGEE---RTKFILQQAHDFEIALQAMDYVLDDNAEVGLKLL 57
MFRVF G+ K + SGEE +TK +L+QA+DFEIAL+AMD+VLDD + GL LL
Sbjct: 1 MFRVF----GSFGSKGNQSSGEEQSTKTKQVLKQANDFEIALKAMDFVLDDRTDEGLNLL 56
Query: 58 ADNQATSPGDATVGALAKGVIEFLEATLGFEPEVMKKASTTLAEAEQLSLKSRAKLQKLN 117
+ + D T+ LA+GVIEFL+ATL FE E MK+A+ TL +AEQ+S KS+ +K N
Sbjct: 57 KKAEMETGSDQTILTLARGVIEFLQATLSFETEEMKRAAITLGKAEQMSWKSKQNAEKTN 116
Query: 118 IKTSSLYPPGTEYAVTYTESCLLHALLMLFSESMVEGAKALFKLRKAYHMLQDILKEINA 177
++SS+YPPGT YAVTYTESCLLHALLMLFSESM+E AKAL KLR+AY MLQDI+ +
Sbjct: 117 FRSSSIYPPGTVYAVTYTESCLLHALLMLFSESMMEAAKALLKLRRAYTMLQDIMVTVKK 176
Query: 178 SEKRKRSSIYL--QEINESTASFISSGTCFTSYDIPYKLTPEEEQDKELLDLANKVYSLR 235
+E+ K SS ++ ES SF+S+ T F S DIPYKL+ E++ + LL+ A K+Y++R
Sbjct: 177 AERSKNSSSPSPSEKSQESCGSFVSAETTFISVDIPYKLSSEDKSNPLLLEFAEKIYTMR 236
Query: 236 RKRLCGAHIGNSPAINRLRDDVGAASLKKGSDEETQEFQLLSDNANINQATMDEFIHSGV 295
RL GAHIGN+P+ +RLRDD+G + + + + +SD+ ++ QAT+DEFIHSG
Sbjct: 237 MGRLSGAHIGNTPSFHRLRDDLGLQT----TPSQASDRHSVSDDFDLEQATIDEFIHSGA 292
Query: 296 NLCFGILQVVLSLIPPAIGAVLSVVGFHGSREEGLRLVWKSTKDRNIHXXXXXXXXXFYY 355
NLC+GILQVVLSL+PPAIGAVLS+VGF GSREEGLRLVWK+TK+RN+H FYY
Sbjct: 293 NLCYGILQVVLSLLPPAIGAVLSIVGFKGSREEGLRLVWKATKERNVHGCIGLLGLMFYY 352
Query: 356 DGPFQFTDIDFDIPA------------ARDDNLPVTEMDRPTLLHPGKILTSALLQARAL 403
DGPFQFTD DFDIP D +L MD TLLHPGKIL ALL+ARAL
Sbjct: 353 DGPFQFTDADFDIPPNDNGSRALNKSRTNDSSLLPGYMDSATLLHPGKILEDALLKARAL 412
Query: 404 FPNSALWLLQEARMLSKQGRLKEAVDLLDSIDHNTIEMKQVKALIVFEKATTLVYMHEFE 463
FPNSALWLL EA+ML+ +GRL++++ L+DSID N+I M+QVK+L+VFE+A LV +HE+
Sbjct: 413 FPNSALWLLNEAKMLAGKGRLRDSLALMDSIDVNSIRMRQVKSLMVFERAILLVNLHEYN 472
Query: 464 RGAETMLIMLSISEWSHALYTYFAGCCYLEMFRKYEMGILNGENXXXXXXXXXXRATNLV 523
R A+ ++ +L IS+WSHALYTYFAGCCYLE +R ++G+LN RA L+
Sbjct: 473 RAADDLISLLDISDWSHALYTYFAGCCYLENWRMTQLGLLNDGKEQFYKE----RARELI 528
Query: 524 FESANMVGKKKFMSKILPLDRFLLRKVDQFKKFQNIIKSSDPLDSIGVSPVHELIYFYNG 583
F++ +++GKK F SK LPLDRF+LRKV QF Q + +PLDSI SPVHEL YFYNG
Sbjct: 529 FDAPSLLGKKTFKSKNLPLDRFMLRKVQQFNNMQKKLNLQEPLDSIATSPVHELAYFYNG 588
Query: 584 YNRMTMKELNLSLKSLTEYRNPTIDLQNPDQELIKDLLTSLVLRRTGKIEEGCKILDTQV 643
YNRMT +L L+ K LTEY NP ID ++PDQELI++LL SL LRR G E G +LD V
Sbjct: 589 YNRMTENDLILTKKMLTEYHNPAIDSEDPDQELIRNLLLSLTLRRLGDAERGLALLDDIV 648
Query: 644 LPQLFTIQNDKVKYIKKTEDPWVYPTAFYERALFSWKLKGMNGLHEAHEWLIRAQNYQDD 703
LP++F IQN KVKY KKTEDPW YP A YERALF WKL GM L+E EWL+RAQNY D
Sbjct: 649 LPKIFYIQNGKVKYFKKTEDPWAYPAALYERALFCWKLGGMESLNECREWLLRAQNYAAD 708
Query: 704 YELSTRIGMKIKAAKDRVEESI 725
YELSTRIGMKIKAA DRVE ++
Sbjct: 709 YELSTRIGMKIKAALDRVENAL 730
>SPAC11E3.14 [S] KOG3783 Uncharacterized conserved protein
Length = 588
Score = 155 bits (393), Expect = 2e-37
Identities = 124/456 (27%), Positives = 213/456 (46%), Gaps = 44/456 (9%)
Query: 268 EETQEFQLLSDNANINQATMDEFIHSGVNLCFGILQVVLSLIPPAIGAVLSVVGFHGSRE 327
+ET E + LS NA +DEFI SGV +G+L ++S+ PP++ +LS+ FHG R+
Sbjct: 173 KETGEIKSLSPNATF----IDEFIESGVITGYGVLTFLVSMFPPSLSRILSLFSFHGVRK 228
Query: 328 EGLRLVWKSTKDRNIHXXXXXXXXXFYYDGPFQFTDIDFDIPAARDDNLPVTEMDRPTLL 387
E L L+W+++K NI F+ ++P + D+ L
Sbjct: 229 ESLELLWRASKYPNIQGAIALLCLYA------------FNAMIQSLGSIPPSNYDQE-LE 275
Query: 388 HPGKILTSALLQARALFPNSALWLLQEARMLSKQGRLKEAVDLLD-SIDHNTIEMKQVKA 446
H A+ R + ALW + EA++ G + A+++ + SID + M+Q+ A
Sbjct: 276 H----CLQAVKDIRKRYSKGALWAVMEAKIYFLTGESQMALEMEELSIDSS---MEQIIA 328
Query: 447 LIVFEKATTLVYMHEFERGAETMLIMLSISEWSHALYTYFAGCCYLEMFRKYEMGILNGE 506
+ F+ A V M +F++ A+ ++ + ++ WSHA Y YFAGCC L+ ++ IL
Sbjct: 329 MKGFDTAMLYVGMRKFKQAADAIIELEDLNSWSHAFYRYFAGCCLLQHGKE----ILGSG 384
Query: 507 NXXXXXXXXXXRATNLVFESANMVGKKKFMSKILPLDRFLLRKVDQFKKFQNIIKSSDPL 566
+ + +V KKK + LP++ +L+RKV +++ + S
Sbjct: 385 GSEEEAKASIDHGIEYLKNAPTLVQKKK-KRRTLPVEAYLIRKVQKWEDRAEKLNIS-IA 442
Query: 567 DSIGVSPVHELIYFYNGYNRMTMKELNLSLKSLTEYRNPTIDLQNPDQELIKDLLTSLVL 626
D++ V P ELIY + + KE L T D+ +K+ L ++
Sbjct: 443 DAMDVPPYAELIYIFVICSLKDPKEAEALRADL-----ETCKCSEEDEAGLKEFLLGVID 497
Query: 627 RRTGKIEEGCKILDTQVLPQLFTIQNDKVKYIKKTEDPWVYPTAFYERALFSWKLKGMNG 686
R K + C++ VL Q D+ + + W+ P A+YE A W + GM
Sbjct: 498 RHL-KEYDSCRVRMEHVL------QLDQGYLSRDNRELWILPFAYYELAALYWDMHGMAA 550
Query: 687 LHEAHEWLIRAQNYQDDYELSTRIGMKIKAAKDRVE 722
E + +L +AQ + +DY+L R+ M +AA ++
Sbjct: 551 EKEVNHYLKKAQEF-NDYDLQNRLSMLAQAATQTLQ 585
Score = 57.0 bits (136), Expect = 1e-07
Identities = 36/146 (24%), Positives = 73/146 (49%), Gaps = 8/146 (5%)
Query: 39 LQAMDYVLDDNAEVGLKLLADNQATSPGDATVGALAKGVIEFLEATLGFEPEVMKKASTT 98
L +D +LDD+A+ +D D A+ + F A LG EP+ +++A+
Sbjct: 38 LYGLDLMLDDDAQGVKSAFSD-------DTVESAVGRSGSAFYNAILGLEPKAIEEATEA 90
Query: 99 LAEAEQLSLKSRAKLQKLNIKTSSLYPPGTEYAVTYTESCLLHALLMLFSESMVEGAKAL 158
L AEQ++ + R K + + + YP G E + ++ L+ + L S+S+V+ KA
Sbjct: 91 LYHAEQVT-EERKKHFEHHDRPIGSYPAGMELQLCLSDIYLMQSALGFVSDSIVDSMKAA 149
Query: 159 FKLRKAYHMLQDILKEINASEKRKRS 184
+++RK + +++ + + +K +
Sbjct: 150 YRIRKTFLSFSKMMEHVRDVQHKKET 175
>SPBC83.16c [S] KOG3783 Uncharacterized conserved protein
Length = 563
Score = 142 bits (358), Expect = 2e-33
Identities = 113/444 (25%), Positives = 201/444 (44%), Gaps = 55/444 (12%)
Query: 288 DEFIHSGVNLCFGILQVVLSLIPPAIGAVLSVVGFHGSREEGLRLVWKSTKDR--NIHXX 345
+E++ +G LC G+ +++SL+PP + +L+V G+ G R+ L+ +W R +
Sbjct: 165 NEYVQTGTLLCTGLFTLLISLLPPKMITILNVFGYKGDRDWALQCMWMPALQRPTSFFAA 224
Query: 346 XXXXXXXFYYDGPFQFTDIDFDIPAARDDNLPVTEMDRPTLLHPGKILTSALLQARALFP 405
YY G Q I P D P K L + P
Sbjct: 225 VAFAALIQYYSGAVQLCSIYKKTPEEPDG-------------WPDKRCFEILEKVEKAHP 271
Query: 406 NSALWLLQEARMLSKQGRLKEAVDLLDSIDHNTI-EMKQVKALIVFEKATTLVYMHEFER 464
+ +W L A++LS + EA+ +L+ + +KQ++ LIVFE A + H +
Sbjct: 272 DGPMWPLHRAKLLSMVKKQDEAIVVLEELMAKPPPRLKQLEVLIVFEHALDCAFSHRYVD 331
Query: 465 GAETMLIMLSISEWSHALYTYFAGCCYLEMFRKYEMGILNGENXXXXXXXXXXRATNLVF 524
GA + L + S+++ S ALY+YFA C+L+ + +A+ L+
Sbjct: 332 GANSFLKLSSLNDSSTALYSYFAAACFLQ------------DVHVNANVEALEKASKLLE 379
Query: 525 ESANMVGKKKFMSKILPLDRFLLRKVDQFKKFQNIIKSSDPL-DSIGVSPVHELIYFYNG 583
++V +K PLD + RKV + K + + L + +G SP++EL+Y +NG
Sbjct: 380 PLHDLVA-----NKTAPLDVHIRRKVGKLIKRRASAGNQGGLAEYVGFSPLYELVYVWNG 434
Query: 584 YNRMTMKELNLSLKSLTEYRNPTIDLQNPDQELIKDLLTSLVLRRTGKIEEGCKILDTQV 643
+ RMT EL+ K E P Q+ D ++ + L+ + VLR G+ +E IL
Sbjct: 435 FRRMTDDELS---KFDVERMEP---WQDQDDDICQALIKATVLRNLGRTDEVFPIL---- 484
Query: 644 LPQLFTIQNDKVKYIKKTEDPWVYPTAFYERALFSWKLKG--MNGLHEAHEWLIRAQNYQ 701
K+ + +T + W A YE A+ ++ G GL +L +A+++
Sbjct: 485 ---------QKICAVTRTTETWAVAFAHYEMAVAFFESNGSKKEGLKHCDAYLRKARDFG 535
Query: 702 DDYELSTRIGMKIKAAKDRVEESI 725
D E +R+ ++++ A+ V + +
Sbjct: 536 GDNEFESRLIIRVQLARHVVRKCL 559
Score = 58.5 bits (140), Expect = 3e-08
Identities = 41/144 (28%), Positives = 70/144 (48%), Gaps = 7/144 (4%)
Query: 30 QQAHDFEIALQAMDYVLDDNAEVGLKLLADNQATSPGDATVGALAKGVIEFLEATLGFEP 89
Q+ +F +Q ++ +LDD K+ + G+++ + V+ F++A L FEP
Sbjct: 7 QRLQNFVTVIQGLNDILDD------KMDEATEKFKSGNSSFHLSGQAVVAFIQAVLTFEP 60
Query: 90 EVMKKASTTLAEAEQLSLKSRAKLQKLNIKTSSLYPPGTEYAVTYTESCLLHALLMLFSE 149
K + + A + + K N S+ + PG EY V+ LL AL+ SE
Sbjct: 61 SRFKDSQNRIDIAIKALSADKDDASKNNTFLST-FDPGVEYRVSIGLMLLLSALIGFCSE 119
Query: 150 SMVEGAKALFKLRKAYHMLQDILK 173
S+V K+++KLRKA+ + I K
Sbjct: 120 SIVTSVKSVYKLRKAHSIFSKINK 143
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.135 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,593,656
Number of Sequences: 60738
Number of extensions: 1596311
Number of successful extensions: 4468
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4447
Number of HSP's gapped (non-prelim): 8
length of query: 725
length of database: 30,389,216
effective HSP length: 114
effective length of query: 611
effective length of database: 23,465,084
effective search space: 14337166324
effective search space used: 14337166324
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)