ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV0619 good M KOG1854 Cell wall/membrane/envelope biogenesis Mitochondrial inner membrane protein (mitofilin)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV0619 198294 196690 -535
(535 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKR016w [M] KOG1854 Mitochondrial inner membrane protein (mitofi... 301 2e-81
SPBC3E7.05c [M] KOG1854 Mitochondrial inner membrane protein (mi... 75 2e-13
Hs5803115 [M] KOG1854 Mitochondrial inner membrane protein (mito... 59 2e-08
CE04961 [M] KOG1854 Mitochondrial inner membrane protein (mitofi... 55 3e-07
YDL058w [U] KOG0946 ER-Golgi vesicle-tethering protein p115 52 2e-06
>YKR016w [M] KOG1854 Mitochondrial inner membrane protein (mitofilin)
Length = 540
Score = 301 bits (771), Expect = 2e-81
Identities = 190/553 (34%), Positives = 297/553 (53%), Gaps = 79/553 (14%)
Query: 20 KTARPFRNFLWKLGAATTVFYVGGVALSTYNYQFAELFTDNVPLAEELVQLVESYNDGTL 79
K + FRN LW + + T FY GG+ S N +F + F++NVP AE+L++ E Y+D
Sbjct: 30 KASHKFRNTLWTIALSATAFYAGGIIYSQKNDKFGDFFSNNVPFAEDLLETYEHYHDRPT 89
Query: 80 NAPQLSLDEIRKKFGSITRKVQSVPHLGSTSSTVTESQSIXXXXXXXXXXXXXXXDXXXX 139
+ S D ++ K + + GS+ + + ++I
Sbjct: 90 LFLEDSWDGLKAKSNDLLSGLT-----GSSQTRRSNRENIEVKKILSLEP---------- 134
Query: 140 XXXXXXXXXXXXXXXXHLKLDDDSNSFNNKSFVESFNKQVDSLNEKEFILPENAVESFLE 199
L ++ +++ K + S N ++SLN+ +PE+ S +
Sbjct: 135 -----------------LNIETENSDPQLKEIIGSLNDLINSLNDSNLSIPESEFNSIKK 177
Query: 200 SYHGLSSQLNELNRDLADQLNSQLGQLSAE----LKQSVESDK---VKEIESNKLQLMQQ 252
S + + L++LN L + L++ + Q ++E L E+ K K ++ N LQ + +
Sbjct: 178 SNQNMLTNLSQLNETLKEALSNYMIQRTSEVITELNTQYENSKREFEKNLQKNLLQEVDE 237
Query: 253 FEKDLSNLKVEFEQKFDSQLQSSLKANEQAMLAKHKNELAMLSIKQVQEFNKIISNKIEN 312
F+++L+ K D +L+ LKANE+ + AKH NE+ +LSI QV+EFNKII +KIE
Sbjct: 238 FKENLTKQK-------DKELEEKLKANEELLQAKHANEVGLLSITQVKEFNKIIKDKIEK 290
Query: 313 ERNGRLKNLDELNGSVKTVSDSLAALEETLLRSECVNQLTNLVSSIKFKLNLDNTPSLDI 372
ERNGRL +L+E+N V +S S+ + L ++E + QLT V IK ++N +N P ++I
Sbjct: 291 ERNGRLAHLEEINSEVNDLSKSIDRSSKILSKNEALVQLTFQVDEIKSRINNNNLPDVNI 350
Query: 373 SKDLQKLTTLVNILP----------------GKPNKCDAKE-------------PQLIDV 403
K+L +L L N+L K NK + KE P L+ V
Sbjct: 351 DKELSRLKLLSNLLSTFNKKSCCDDGDCCSCKKGNKNEGKEGKISCKCKPKTNPPSLLSV 410
Query: 404 VVNELNSLTSAKENKQILSNEQLLNRWGLLQDKIREASLLPPNAGFLGHVSAKFFSLFLF 463
++EL S S K+ILSNEQ+ NRW LL D + ASLLPPN+G LG ++AK FSLFLF
Sbjct: 411 ALDELESTCS---GKKILSNEQIYNRWNLLADDFKTASLLPPNSGILGQLTAKVFSLFLF 467
Query: 464 NKSG-ISNENDIDSVISRVTENIKLNRLDKAVEDVVQLQGWSRLEADDWLQAARSKLELE 522
K+G SN D DSV +RV +N++++ L+ AVE+VV L+GW + W++ AR KLE++
Sbjct: 468 TKTGNPSNATDFDSVYARVGDNLRVSNLNDAVEEVVSLKGWPHKVCESWIEDARRKLEVQ 527
Query: 523 TLVDVVDHEIKTL 535
LV+++D EI+TL
Sbjct: 528 RLVEILDCEIRTL 540
>SPBC3E7.05c [M] KOG1854 Mitochondrial inner membrane protein (mitofilin)
Length = 550
Score = 75.1 bits (183), Expect = 2e-13
Identities = 82/340 (24%), Positives = 156/340 (45%), Gaps = 49/340 (14%)
Query: 206 SQLNELNRDLADQLNSQLGQLSAELKQSVES---DKVKEIESNKLQLM----QQFEKDLS 258
S LNE N +L+ +L ++ E + E +KV E+ES L+ ++++
Sbjct: 227 SSLNEAN-----ELHGKLSKIQQEQEHLFEQRLREKVSEMESKLEALLIARDEKWQSAFE 281
Query: 259 NLKVEFEQKFDSQLQSSLKANEQAMLAKHKNELAMLSIKQVQEFNKIISNKIENERNGRL 318
+ K+ ++ +++LQ L +K KNEL +I + + I ++E ER RL
Sbjct: 282 SEKLRLQKLHEARLQQELFKLASVFESKLKNELTEQAITLEKLHLQSIKAQVEQERGSRL 341
Query: 319 KNLDELNGSVKTVSDSLAALEETLLRSECVNQLTNLVSSIKFKLNLDNTPSLDISKDLQ- 377
L EL S + + + L +L ++T LV ++ NT D++KD++
Sbjct: 342 GRLQELRNSFQQLQE----LVRVVLHEN--GRVTRLV-------DVSNTLD-DLNKDMRF 387
Query: 378 -KLTTL---VNILPG--KPNKCDAKEPQLIDVVVNELNSLTSAKENKQILSNEQLLNRWG 431
KL+ + VN L K ++ A ++I+ +V ++ IL E+L ++
Sbjct: 388 HKLSEVRQHVNTLKEATKDDELAALASRVIEKIV----------DSGPILDKEELQTKFD 437
Query: 432 LLQDKIREASLLPPNAGFLGHVSAKFFSLFLFNKSGISNENDIDSV---ISRVTENIKLN 488
L +I + L +GF GH+ + S + + DI SV + ++ +
Sbjct: 438 TLSKEIYKTCFLTTESGFFGHLKSIILSQL---PAAVFKSPDIVSVKKTLEDARSHLLKD 494
Query: 489 RLDKAVEDVVQLQGWSRLEADDWLQAARSKLELETLVDVV 528
LD +V ++ L W R + DW+ A R ++EL+ ++++
Sbjct: 495 DLDGSVRALLSLSQWPRALSRDWINACRRRMELQQAIEII 534
>Hs5803115 [M] KOG1854 Mitochondrial inner membrane protein (mitofilin)
Length = 758
Score = 58.9 bits (141), Expect = 2e-08
Identities = 73/350 (20%), Positives = 153/350 (42%), Gaps = 47/350 (13%)
Query: 207 QLNELNRDLADQLNSQ-------LGQLSAELKQSVESDKVKEIESNKLQLMQQFEKDLSN 259
++++LNR+LA+Q ++ L + E K++ +S K +E ++ ++ + ++ +
Sbjct: 411 RIDQLNRELAEQKATEKQHITLALEKQKLEEKRAFDSAVAKALEHHRSEIQAEQDRKIEE 470
Query: 260 LKVEFEQKFDSQLQSS-----------LKANEQAMLAKHKNELAMLSIKQVQEFNKIISN 308
++ E + +QL+ L+ EQ + ++ + L+ +Q +F ++
Sbjct: 471 VRDAMENEMRTQLRRQAAAHTDHLRDVLRVQEQELKSEFEQNLSEKLSEQELQFRRLSQE 530
Query: 309 KIENERNGRLKNLDELNGSVKTVSDSLAALEETLLRSECVNQLTNLVSSIKFKLNLDN-- 366
+++N L G + V A EE + +QL V ++K+ + +
Sbjct: 531 QVDNFTLDINTAYARLRGIEQAVQSHAVAEEE----ARKAHQLWLSVEALKYSMKTSSAE 586
Query: 367 TPSLDISKDLQKLTTLVNILPGKPNKCDAKEPQLIDVVVNELNSLTSAKENKQILSNEQL 426
TP++ + ++ + K N D + Q + + SLT + + S E L
Sbjct: 587 TPTIPLGSAVEAI---------KANCSDNEFTQALTAAIPP-ESLT-----RGVYSEETL 631
Query: 427 LNRWGLLQDKIREASLLPPNAGFLGHVSAKFF-SLFLFNKSGISNE-----NDIDS--VI 478
R+ +Q R +++ L + SL LF + DI++ ++
Sbjct: 632 RARFYAVQKLARRVAMIDETRNSLYQYFLSYLQSLLLFPPQQLKPPPELCPEDINTFKLL 691
Query: 479 SRVTENIKLNRLDKAVEDVVQLQGWSRLEADDWLQAARSKLELETLVDVV 528
S + I+ L+ A + V QL+G SR A DWL+ AR LE + +V+++
Sbjct: 692 SYASYCIEHGDLELAAKFVNQLKGESRRVAQDWLKEARMTLETKQIVEIL 741
>CE04961 [M] KOG1854 Mitochondrial inner membrane protein (mitofilin)
Length = 727
Score = 54.7 bits (130), Expect = 3e-07
Identities = 102/409 (24%), Positives = 163/409 (38%), Gaps = 59/409 (14%)
Query: 161 DDSNSFNNKSFVES--FNKQVDSLNE--KEFILPENAVESFLESYHGLSSQLNE--LNRD 214
D+ N+ NKS ES N+ D + + ++F L ++ ++ H LNE LN
Sbjct: 324 DEINALVNKSRQESAVLNQYKDLIEKSRQQFALEMKSILPNVD-IHAKDKNLNEDELNAL 382
Query: 215 LA------DQLNSQLG--QLSAEL--KQSVESDKVKE--IESNKLQLM-----QQFEKDL 257
+A DQL QL Q+ EL +++E ++ + I S KL + +Q E ++
Sbjct: 383 IAHAHLKVDQLRCQLSDQQVREELHISKALEEQRLADERIASEKLSIEMSRVGRQNELEI 442
Query: 258 SNLKVEFEQKFDSQLQSSLKANEQAMLAKHKNELAMLSIKQVQEF----NKIISNKIENE 313
VE ++ +L++ LK A H L + Q Q F N+ + + E
Sbjct: 443 ERALVESRSSWEGELENQLKRTASA----HSEHLEQVIRTQRQLFEIEQNQKVEEAVLQE 498
Query: 314 RNGRLKN----LDELNGSVKTVSDSLAALEETLLRSECVNQLTNLVSSIKFKLNLDNTPS 369
RN K L L G + + +A E + NL+ ++K N
Sbjct: 499 RNLHSKQVGAALSRLEGIEEALGSRVALDNENRRAKQFWIACHNLIDTLKH----GNKAG 554
Query: 370 LDISKDLQKLTTLVNILPGKPNKCDAKEPQLIDVVVNEL-NSLTSAKENKQILSNEQLLN 428
+I + L +N+L KE D VN + +S + + L N
Sbjct: 555 NNIDERRLPLNESLNLL---------KEVNPEDEFVNAIIDSFPKQATTVGTYTEQDLKN 605
Query: 429 RWGLLQDKIREASLLPPNAGFLGHVSAKFF-SLFLF-------NKSGIS-NENDIDSVIS 479
R+ L R+ + + N G LG + SLFL N I N D ++S
Sbjct: 606 RFEQLYKIGRKTASIDENGGTLGAYFWSYVKSLFLVDMPQQYGNLDAIDVNNTDNYEILS 665
Query: 480 RVTENIKLNRLDKAVEDVVQLQGWSRLEADDWLQAARSKLELETLVDVV 528
R + + LDKA+ V L+G A DW+ RS LE L ++
Sbjct: 666 RAKQYVHNGDLDKAIRVVQLLKGQPAHLARDWIVDTRSYLESRLLAQLL 714
>YDL058w [U] KOG0946 ER-Golgi vesicle-tethering protein p115
Length = 1790
Score = 52.0 bits (123), Expect = 2e-06
Identities = 88/401 (21%), Positives = 175/401 (42%), Gaps = 55/401 (13%)
Query: 157 LKLDDDSNSFNNKSFVESFNKQVDSLNEKEFILPENAVESFLESYHGLSSQLNELNRDLA 216
+K + S KS +++ N Q+ L +K E S LES + S+ ++ ++L
Sbjct: 1210 MKSTSEEQSNLKKSEIDALNLQIKELKKKN----ETNEASLLESIKSVESETVKI-KELQ 1264
Query: 217 DQLN---SQLGQLSAELKQSV-----------ESDKVK-EIESNKLQLMQQFEK--DLSN 259
D+ N ++ +L +LK S ES+K+K E+++ +L Q EK +LS
Sbjct: 1265 DECNFKEKEVSELEDKLKASEDKNSKYLELQKESEKIKEELDAKTTELKIQLEKITNLSK 1324
Query: 260 LKVEFEQKFD--SQLQSSLKANEQAMLAKHKNELAMLSIKQVQEFNKIISNKIENERNGR 317
K + E + + S + N + L K KNE+ + + Q F K K+ NE +
Sbjct: 1325 AKEKSESELSRLKKTSSEERKNAEEQLEKLKNEIQIKN----QAFEK--ERKLLNEGSST 1378
Query: 318 LKNLDELNGSVKTVSDSLAALE-ETLLRSECVNQLTNLVSSIKFKLNLDNTPSLDISKDL 376
+ E + + T+ D L L+ E L+++ ++ N S ++ K++L N L+ ++
Sbjct: 1379 I--TQEYSEKINTLEDELIRLQNENELKAK---EIDNTRSELE-KVSLSNDELLEEKQNT 1432
Query: 377 QKLTTLVNILPGKPNKCDAKEPQLIDVVVNELNSLTSAKENKQILSNEQLLNRWGLL--- 433
K +L + + +K + +L+ + + L S KE + + GL
Sbjct: 1433 IK--SLQDEILSYKDKITRNDEKLLSIERDNKRDLESLKEQLRAAQESKAKVEEGLKKLE 1490
Query: 434 QDKIREASLLPPNAGFLGHVSAKFFSLFLFNKSGISNENDIDSVISR----VTENIK--- 486
++ +E + L + + + + S KS + D + + E+IK
Sbjct: 1491 EESSKEKAELEKSKEMMKKLESTIESNETELKSSMETIRKSDEKLEQSKKSAEEDIKNLQ 1550
Query: 487 ------LNRLDKAVEDVVQLQGWSRLEADDWLQAARSKLEL 521
++R++++ +D+ +L+ R+EA + K EL
Sbjct: 1551 HEKSDLISRINESEKDIEELKSKLRIEAKSGSELETVKQEL 1591
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.312 0.129 0.344
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,955,803
Number of Sequences: 60738
Number of extensions: 991344
Number of successful extensions: 3948
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 3937
Number of HSP's gapped (non-prelim): 9
length of query: 535
length of database: 30,389,216
effective HSP length: 111
effective length of query: 424
effective length of database: 23,647,298
effective search space: 10026454352
effective search space used: 10026454352
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)