ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV0686 good O KOG2164 Posttranslational modification, protein turnover, chaperones Predicted E3 ubiquitin ligase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV0686 217149 215293 -619
(619 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR427w [O] KOG2164 Predicted E3 ubiquitin ligase 470 e-132
SPBP8B7.23 [O] KOG2164 Predicted E3 ubiquitin ligase 199 1e-50
At3g26730 [O] KOG2164 Predicted E3 ubiquitin ligase 83 1e-15
Hs7662653 [O] KOG2164 Predicted E3 ubiquitin ligase 81 5e-15
7292099 [O] KOG2164 Predicted E3 ubiquitin ligase 80 7e-15
>YLR427w [O] KOG2164 Predicted E3 ubiquitin ligase
Length = 670
Score = 470 bits (1209), Expect = e-132
Identities = 243/548 (44%), Positives = 353/548 (64%), Gaps = 26/548 (4%)
Query: 64 EDLEWNIQDELAHGDYKLNGKKTKISINHLLDFQLPEVERFKSGSTRAPDKXXXXXQSDN 123
+D++ +IQ+E+ G++KL G+KT++SINHLL+FQLPEVER KS S+ +
Sbjct: 88 QDIDLSIQEEILGGNFKLRGRKTQVSINHLLNFQLPEVEREKSRSSSSKKSNRR-----R 142
Query: 124 QDRVYLHGDSFVNANYKFLVDDEGSYEAQCNDPNVPLETESVKRVVIPKGQTCPICLSED 183
+ V+LHGD+FVN NY+ LVDD Y Q +PNVP++ E + RV++PKGQ C ICLSE+
Sbjct: 143 DEHVHLHGDTFVNVNYRLLVDDRFDYPEQNCNPNVPVDQEKILRVIVPKGQNCSICLSEE 202
Query: 184 LISPRMVVCGHVFCQTCLLQLFTNDPMVGKNDESTYARMKRKDLRECPLCSSIIKKTSVK 243
++PRMV CGH+FC +CLL F+ + V KN E+ Y+ K+K +ECPLC SII VK
Sbjct: 203 PVAPRMVTCGHIFCLSCLLNFFSIEETV-KNKETGYS--KKKKYKECPLCGSIIGPKRVK 259
Query: 244 PVLFTD---ADSKDNTPTIGSMVELQLMCRPHGSLLPLPVNLNIDPFSIGNFPSVHMKEL 300
PVL+ D + P G+ V LQLMC+PHGSLLPLPV L++DP GNFP ++ +
Sbjct: 260 PVLYEDDFDVTRLNQKPEPGATVHLQLMCKPHGSLLPLPVALHLDPLKCGNFPPANLGSI 319
Query: 301 AVYSRIMKCSTQYAIHLLERDNLQIYEQLEIDKALYHDNGKLAQLALDENVSLINGLKSK 360
Y+ IMKC Y++ L ++D + I EQ EIDKA+Y+D+GK + +++ I+ L +
Sbjct: 320 KHYAHIMKCGVSYSLELYQKDIVAIQEQYEIDKAIYNDSGKFVKQSIENINDQISTLLAA 379
Query: 361 NNNKEP-------DISALSIASGGLEKYNDDNAFFYYEAGFNSTTKFYLSPLDVKVLKNV 413
+ P + L KY+D +A+F+Y+ S+TK++LSPLDVK+L +
Sbjct: 380 TTDLSPLSNDINNGLDNFHFDDDLLTKYDDSSAYFFYQTLVASSTKYFLSPLDVKILLTI 439
Query: 414 FAHYHEFPSTIKIKVEDIHYGSMVTETTISKFKYFSHLPIGTELAFVDLDWRNEDIIPKE 473
F +Y +FP +I+ VE+IHY ++VTE I ++KY HLPIGTE+A +DLDWR +PKE
Sbjct: 440 FHYYSKFPESIETTVENIHYDTVVTEQLIRRYKYIGHLPIGTEIALLDLDWRKIPFLPKE 499
Query: 474 VYNKFFAELRQRHRKAMTKRNKEDKDKITYQERLEQEHLEFYQRENNN--QYPPMQKPAT 531
+Y +F EL+QR RK K+ KEDK+K Y++RLEQEH EFY++EN N ++ + AT
Sbjct: 500 IYEQFAHELKQRRRKFTMKKQKEDKEKKLYEKRLEQEHAEFYRKENGNSLKFEDSVQMAT 559
Query: 532 QFPSKKTSTVNHPS-NIALSASPDDEASS----KPKMKERTVWGTQITVT-DQNVIEEDL 585
+ S +S++ S I++ P + S+ P +RT+WGT I VT D+ +E+
Sbjct: 560 HYESIVSSSIPLNSLGISMLGPPTNSCSTPQKQAPSHTKRTIWGTSIAVTEDEKASKENK 619
Query: 586 EFEKMLLE 593
EF+ MLL+
Sbjct: 620 EFQDMLLQ 627
>SPBP8B7.23 [O] KOG2164 Predicted E3 ubiquitin ligase
Length = 673
Score = 199 bits (505), Expect = 1e-50
Identities = 160/527 (30%), Positives = 258/527 (48%), Gaps = 49/527 (9%)
Query: 84 KKTKISINHLLDFQLPEVERFKSGSTRAPDKXXXXXQSDNQDRVYLHGDS--FVNANYKF 141
K+ +I++NHLL+FQ R S + AP + + Q + D +VNANY+F
Sbjct: 119 KRGQINLNHLLNFQF--TPRTNSNAFSAPPRRSRGYNTYGQGSGHHPMDKSRYVNANYRF 176
Query: 142 LVDDEGSYEAQCNDPNVPLETESVKRVVIPKG---QTCPICLSEDLISPRMVVCGHVFCQ 198
+V G Y++Q DP+ P++ E V +V+ CP CL E ++ RM CGHV+C
Sbjct: 177 VVSPIGDYQSQKLDPDSPVKWEDVWQVLCSSDFQLAACPFCLEEKPVAARMSRCGHVYCF 236
Query: 199 TCLLQLFTNDPMVG--KNDESTYARMKRKDLRECPLCSSIIKKTSVKPVLFTDADSKDNT 256
+CLL+ F P K E++ ++ + R CP+C I+ V P+ + + D +
Sbjct: 237 SCLLR-FVETPTAAEVKAAETSGTKIVKCGHRSCPICWDSIRLRDVHPIRWVE-DKEFQK 294
Query: 257 PTIGSMVELQLMCRPHGSLLPLPVNLNIDPFSI-GNFPSVHMKEL----AVYSRIMKCST 311
G V L+L R +GS+L P + F++ G+F S + A Y+RI+ S
Sbjct: 295 LEEGKSVCLRLYQRNNGSILAFPRSCR--SFALDGSFHSDEIPNFTMSGAAYARIIIGSH 352
Query: 312 QYAI--HLLERDNLQIYEQLEIDKALYHDN--GKLAQLALDENVSLINGLKSKN-NNKEP 366
Y + HLLE + LQ +I D ++ Q+ LD SL + +N + +
Sbjct: 353 DYMVQQHLLEIEQLQQIAAEDISLYGSADTYYERITQILLDRISSLSESVNEQNIKSLQT 412
Query: 367 DISALSIASGGLEKYN--DD----------NAFFYYEAGFNSTTKFYLSPLDVKVLKNVF 414
DI L + S L++ + DD +A+ +Y+ +S YLSPLD+++LK+ F
Sbjct: 413 DIDNLCLQSNSLKQLSEVDDLNDVSGSEIADAYLFYQPFAHS--HIYLSPLDIRILKSAF 470
Query: 415 AHYHEFPSTIKIKVEDIHYGSMVTETTISKFKYFSHLPIGTELAFVDLDWRNEDIIPKEV 474
Y FP + +VE I G +V +FKY +HLP G E+AF++ DW IIPKEV
Sbjct: 471 GSYENFPDELVPRVERISSGHLVNSELRQRFKYMAHLPEGCEVAFIECDW--SKIIPKEV 528
Query: 475 YNKFFAELRQRHRKAMTKRNKEDKDKITYQERLEQEHLEFYQRENNNQYPPMQKPATQFP 534
F +E+ +R ++ + +E++ Y++R E++ E E N Q P + + P
Sbjct: 529 LLTFKSEISKRRKQRKAQEMREER----YRQRAERDTEEQIYSELNMQRPVPKTVVHEDP 584
Query: 535 SKKTSTV--NHPSNIALSASPDDEASSKPKMKERTVWGTQITVTDQN 579
++ T+ +H + D+ S K TVWGT+ QN
Sbjct: 585 AEAFPTLGSHHQFESTDEVNTDESTLSTAK----TVWGTRALANIQN 627
>At3g26730 [O] KOG2164 Predicted E3 ubiquitin ligase
Length = 769
Score = 83.2 bits (204), Expect = 1e-15
Identities = 122/550 (22%), Positives = 214/550 (38%), Gaps = 113/550 (20%)
Query: 88 ISINHLLDFQLPEVERFKSGSTRAPDKXXXXXQSDNQDRVYLHGDSFVNANYKFLVDDEG 147
IS NHLL+FQ + + +S P + + + R + + D F+ ANYKF+V D G
Sbjct: 161 ISGNHLLNFQYDPISQPQSRGPPPPQR-----RQQYKGRPF-NKDLFLKANYKFVVLDTG 214
Query: 148 SYEAQCNDPNVPLETESV--KRVVIPKGQTCPICLSEDLISPRMVVCGHVFCQTCLLQLF 205
+ DP+ L+ + + R P CPICL E + P++ CGH+FC C+LQ
Sbjct: 215 DHSPDSMDPDKMLQWDDIICVRYSTPSPVQCPICL-EYPLCPQITSCGHIFCFPCILQYL 273
Query: 206 TNDPMVGKNDESTYARMKRKDLRECPLCSSIIKKTSVKPVLFTDADSKDNTPTIGSMVEL 265
+ G ++ K + CPLC +I + V + ++G +E
Sbjct: 274 ----LTGVDNH------KVDCFKRCPLCFVMISPRELYTVYIENVKQY----SVGDPIEF 319
Query: 266 QLMCRPHGSLLPLPVNLN-----------IDPFSIGNFPSVHMKELAVYSRIMKCSTQYA 314
L+ R S P N + DPFS F +L+V + + + A
Sbjct: 320 VLLTRKKDSFAPTRKNEHDLAVPNGENEIYDPFS--KFTFTQDVDLSVRQAVSELDSWIA 377
Query: 315 ---IHLLERDNLQIY-----EQLEIDKALYHDN-----GKLAQLALDENVSLI------- 354
L+E +Y E+LE K ++++ K+ A ++ SL
Sbjct: 378 RADPQLVEDLEKHLYVNAALERLEQRKLYWNEHKLSIYSKVCTTARNQTQSLSPPDVSRV 437
Query: 355 --------NGLKSKNNN---KEPDISALSIASG----GLEKYNDDNAFFYYE-------A 392
+G +N K + S+ + G LEK D++ E +
Sbjct: 438 GYQAPMGGHGAPDSGSNDQDKSTEDSSADKSDGESQSSLEKGCDNDLGHPLEDQDAPSSS 497
Query: 393 GFNSTTKFYLSPLDVKVLKNVFAH--------YHEFPSTIKIKVEDIHYGS--------- 435
N + F +D K LK+ A+ H T+ +K HYGS
Sbjct: 498 SCNGSKGFLSHQIDTKDLKDNDAYNFYQSVDGQHIILHTLNVKCLLHHYGSYDFLPPRLS 557
Query: 436 ---------MVTETTISKFKYFSHLPIGTELAFVDLDWRNEDIIPKEVYNKFFAELRQRH 486
+E ++++ SH + T L +D+ ++ +P + + F E+++R
Sbjct: 558 GKILEMETITQSEAIRRRYRFLSHFSLSTTLQ-IDM----KESLPPDAFAPFIHEIKKRE 612
Query: 487 RKAMTKRNKEDKDKITYQERLEQEHL----EFYQRENNNQYPPMQKPATQFPSKKTSTVN 542
++ + K+ K+KI + E + F Q +++ +PP + +
Sbjct: 613 KQRKERAWKDRKNKIKAEVAAAAELVPLMASFGQSSSSDDFPPFSLDDFEALGNSAPISS 672
Query: 543 HPSNIALSAS 552
P NI +S
Sbjct: 673 SPPNIGERSS 682
>Hs7662653 [O] KOG2164 Predicted E3 ubiquitin ligase
Length = 761
Score = 80.9 bits (198), Expect = 5e-15
Identities = 63/253 (24%), Positives = 124/253 (48%), Gaps = 35/253 (13%)
Query: 87 KISINHLLDFQLPEVERFKSGSTRAPDKXXXXXQSDNQDRVYLHGDSFVNANYKFLVDDE 146
KI++NHLL+F R ++G ++ + + + + F+ AN +F+V ++
Sbjct: 87 KINLNHLLNFTFEP--RGQTGHFEGSGHGSWGKRNKWGHKPF-NKELFLQANCQFVVSED 143
Query: 147 GSYEAQCNDPNVPLETESVKRVVIPKGQ--TCPICLSEDLISPRMVVCGHVFCQTCLLQL 204
Y A DP+ + + V++V I + +CPICL + ++ CGH+FC C+L
Sbjct: 144 QDYTAHFADPDTLVNWDFVEQVRICSHEVPSCPICLYPPTAA-KITRCGHIFCWACILH- 201
Query: 205 FTNDPMVGKNDESTYARMKRKDLRECPLCSSIIKKTSVKPVLFTDADSKDNTPTIGSMVE 264
Y + K +CP+C S + K +K V+ T++ + +G +
Sbjct: 202 --------------YLSLSEKTWSKCPICYSSVHKKDLKSVVATES----HQYVVGDTIT 243
Query: 265 LQLMCRPHGSLLPLPVN--LNID-PFSIGNFPSVHMKELAVYSRIMKCSTQYAIH-LLER 320
+QLM R G L+ LP + +N+D P +G+ ++ + YS+++ S + +H ++
Sbjct: 244 MQLMKREKGVLVALPKSKWMNVDHPIHLGD------EQHSQYSKLLLASKEQVLHRVVLE 297
Query: 321 DNLQIYEQLEIDK 333
+ + + +QL +K
Sbjct: 298 EKVALEQQLAEEK 310
Score = 53.1 bits (126), Expect = 1e-06
Identities = 43/193 (22%), Positives = 80/193 (41%), Gaps = 25/193 (12%)
Query: 373 IASGGLEKYNDDNAFFYYEAGFNSTTKFYLSPLDVKVLKNVFAHYHEFPSTIKIKVEDIH 432
I G + + +++Y+A +L P++V+ L + P I V +I
Sbjct: 442 ITKSGFTRLSSSPCYYFYQA--EDGQHMFLHPVNVRCLVREYGSLERSPEKISATVVEIA 499
Query: 433 YGSMVTETTISKFKYFSHLPIGTELAFVDLDWRNEDIIPKEVYNKFFAELRQRHRKAMTK 492
G ++E + +Y SHLP+ E + +L + ++ KE F ++ +R
Sbjct: 500 -GYSMSEDVRQRHRYLSHLPLTCEFSICELALQ-PPVVSKETLEMFSDDIEKR------- 550
Query: 493 RNKEDKDKITYQERLEQEHLEFYQRENNNQYPPMQKP---ATQFPSKKTSTVNHPSNI-- 547
K + K +ER + +E + + +YP + P QFP+ + T + S +
Sbjct: 551 --KRQRQKKAREERRRERRIEIEENKKQGKYPEVHIPLENLQQFPAFNSYTCSSDSALGP 608
Query: 548 -------ALSASP 553
ALS SP
Sbjct: 609 TSTEGHGALSISP 621
>7292099 [O] KOG2164 Predicted E3 ubiquitin ligase
Length = 708
Score = 80.5 bits (197), Expect = 7e-15
Identities = 96/450 (21%), Positives = 186/450 (41%), Gaps = 58/450 (12%)
Query: 78 DYKLNGKKT----KISINHLLDFQLPEVERFKSGSTRAPDKXXXXXQSDNQDRVYLHGDS 133
D++LN T K ++NHLL+F E + +A + ++ +
Sbjct: 114 DHELNSVYTQGSKKQNLNHLLNFHCVPRELERGHHHQAQQHHGLSGRKQRYNK-----EQ 168
Query: 134 FVNANYKFLVDDEGSYEAQCN-DPNVPLETESVKRVVIPKGQT--CPICLSEDLISPRMV 190
F+ AN++F++ +AQ N P+ ++ ++++ I + CPICL ++ ++
Sbjct: 169 FLQANFQFVI--RSGAKAQVNGSPDALIDWSYIEQINIQTTEELQCPICLYPP-VAAKLT 225
Query: 191 VCGHVFCQTCLLQLFTNDPMVGKNDESTYARMKRKDLRECPLCSSIIKKTSVKPVLFTDA 250
CGH +C CLL Y + K R+CP+C I +K
Sbjct: 226 RCGHAYCWPCLLH---------------YLSLSDKTWRKCPICYDAIHAGDLKSCTIEQL 270
Query: 251 DSKDNTPTIGSMVELQLMCRPHGSLLPLPVNLNIDPFSIGNFPSVHMKELAV-YSRIMKC 309
+G + QLM R GS+ + +I FP V E A YS+ +
Sbjct: 271 RDLQ----VGEKITFQLMRRRKGSMYIENHVAGLGE-TIERFPFVSAGEEAKRYSKFLIA 325
Query: 310 STQYAIHLLERDNLQIY--------EQLEIDKALYHDNGKLAQLALD--------ENVSL 353
++ER+ ++ E + I +AL ++ +L ++ E +
Sbjct: 326 KRMDVAAIIERERNELLAESDVSCPEDVFIQQALVMLQERVEKLGVEKPDPKEDEEETAP 385
Query: 354 INGLKSKNN---NKEPDISALSIASGGLEKYNDDNAFFYYEAGFNSTTKFYLSPLDVKVL 410
+ L+++ K D S S + Y+ ++ +YY N YL PL+VK+L
Sbjct: 386 VLCLETETKIDIEKSDDASISSGEASSSLSYSSNHNKYYYFYQSNDGQNIYLHPLNVKML 445
Query: 411 KNVFAHYHEFPSTIKIKVEDIHYGSMVTETTISKFKYFSHLPIGTELAFVDLDWRNEDII 470
+ + P I+ ++ + SM E KF HLP+ + + V+++ + ++
Sbjct: 446 QACYGTLDLGPLLIEAQIVQMEQHSM-DEEHRRKFTCLGHLPLTCQFSVVEVELQ-PPVV 503
Query: 471 PKEVYNKFFAELRQRHRKAMTKRNKEDKDK 500
+ F ++ R +K +R++E++ +
Sbjct: 504 TGGILKLFKEDILHR-KKERQRRDREERKR 532
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.133 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,912,616
Number of Sequences: 60738
Number of extensions: 1722828
Number of successful extensions: 4533
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 4510
Number of HSP's gapped (non-prelim): 8
length of query: 619
length of database: 30,389,216
effective HSP length: 112
effective length of query: 507
effective length of database: 23,586,560
effective search space: 11958385920
effective search space used: 11958385920
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)