ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIV0715   good          S     KOG4149  Function unknown                                               Uncharacterized conserved protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIV0715  229566  230783 406  
         (406 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YKL195w [S] KOG4149 Uncharacterized conserved protein                 148   2e-35
SPAC57A10.11c [S] KOG4149 Uncharacterized conserved protein           126   7e-29
Hs21389469 [S] KOG4149 Uncharacterized conserved protein               99   1e-20

>YKL195w [S] KOG4149 Uncharacterized conserved protein
          Length = 427

 Score =  148 bits (373), Expect = 2e-35
 Identities = 89/253 (35%), Positives = 124/253 (48%), Gaps = 52/253 (20%)

Query: 85  SEPTKDADESPVSAQEEGAEPVTTPENEITYSEETHQALAATLSGDEPAAIEPVAESVNE 144
           S   KD DES VS +++               +       A    DE  + E   E+ ++
Sbjct: 175 SSEDKDTDESKVSTKDD--------------EQSNEDNATANNQKDENISSENSEENTSD 220

Query: 145 QATEPAASGEATNEPVTGISEDTKAPSLSFDDSKTAKGVVLEDEADKKEIQQTSPDAVKT 204
           +  +  A      +P    S+D K+     DD  T +    E+E+ KK +  +   A ++
Sbjct: 221 KTLDNNAGSSEKKDPEH--SDDEKSQQGQSDDKTTTEDNNGEEESSKKTVSDSENSAKQS 278

Query: 205 ASKDGSEGESDVVLHEKSPXXXXXXXXXXXXXXXRSIXXXXXXXXXXXXXXXXXXXXXKK 264
            S D  E + ++   E+                                         ++
Sbjct: 279 ESSD--EEKEELRKQEEKQMGPTE----------------------------------EE 302

Query: 265 NEQQTAYNPETGEINWDCPCLGGMAYGPCGEEFKSAFSCFVYSEADPKGINCVEKFSTMQ 324
            + + AYNP+TGEINWDCPCLGGMA+GPCGEEFKSAFSCFVYSEA+PKGI+CVEKF  MQ
Sbjct: 303 VQHEGAYNPDTGEINWDCPCLGGMAHGPCGEEFKSAFSCFVYSEAEPKGIDCVEKFQHMQ 362

Query: 325 NCFRKYPDYYAEQ 337
           +CFRKYP++YAEQ
Sbjct: 363 DCFRKYPEHYAEQ 375


>SPAC57A10.11c [S] KOG4149 Uncharacterized conserved protein
          Length = 313

 Score =  126 bits (316), Expect = 7e-29
 Identities = 65/190 (34%), Positives = 97/190 (50%), Gaps = 11/190 (5%)

Query: 147 TEPAASGEATNEPVTGISEDTKAPSLSFDDSKTAKGVVLEDEADKKEIQQTSPDAVKTAS 206
           T  A+S +  + PV G    T+  S +    +  +   +ED  +KK   +T P   +   
Sbjct: 75  TSNASSSQDNDHPVVGEHVHTETQSYNEPYMQRHR---IEDAIEKKMTSETQPSTDEKGR 131

Query: 207 KDGSEGESDVVLHEKSPXXXXXXXXXXXXXXXRSIXXXXXXXXXXXXXXXXXXXXXKKNE 266
           K        V   E S                  +                     +++ 
Sbjct: 132 K--------VSATENSAPKKTDKEKSSGETAGNILREQIATGKDDDEYARKFEEVEEESS 183

Query: 267 QQTAYNPETGEINWDCPCLGGMAYGPCGEEFKSAFSCFVYSEADPKGINCVEKFSTMQNC 326
           +++A+NP+TGEINWDCPCLGGMA+GPCGEEFK+AFSCFVYS+++PKG+ C++KF  MQ C
Sbjct: 184 EESAFNPDTGEINWDCPCLGGMAHGPCGEEFKAAFSCFVYSKSEPKGMECLDKFQAMQAC 243

Query: 327 FRKYPDYYAE 336
           F+K+P+ Y +
Sbjct: 244 FQKHPEIYQD 253


>Hs21389469 [S] KOG4149 Uncharacterized conserved protein
          Length = 155

 Score = 99.0 bits (245), Expect = 1e-20
 Identities = 40/62 (64%), Positives = 49/62 (78%)

Query: 276 GEINWDCPCLGGMAYGPCGEEFKSAFSCFVYSEADPKGINCVEKFSTMQNCFRKYPDYYA 335
           G INW+CPCLGGMA GPCGE+FKSAFSCF YS  + KG +CV++F  MQ C +KYPD Y 
Sbjct: 60  GNINWNCPCLGGMASGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYP 119

Query: 336 EQ 337
           ++
Sbjct: 120 QE 121


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.307    0.125    0.353 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,018,856
Number of Sequences: 60738
Number of extensions: 717185
Number of successful extensions: 2609
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2604
Number of HSP's gapped (non-prelim): 5
length of query: 406
length of database: 30,389,216
effective HSP length: 109
effective length of query: 297
effective length of database: 23,768,774
effective search space: 7059325878
effective search space used: 7059325878
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)