ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV0732 good R KOG2105 General function prediction only Predicted metal-dependent hydrolase, contains AlaS domain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV0732 237846 236491 -452
(452 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL040w [R] KOG2105 Predicted metal-dependent hydrolase contains... 388 e-108
Hs13376886_2 [R] KOG2105 Predicted metal-dependent hydrolase con... 159 1e-38
7290417 [R] KOG2105 Predicted metal-dependent hydrolase contains... 128 2e-29
Hs22064738 [R] KOG2105 Predicted metal-dependent hydrolase conta... 94 4e-19
Hs20555071 [J] KOG0188 Alanyl-tRNA synthetase 54 5e-07
7295490 [J] KOG0188 Alanyl-tRNA synthetase 54 6e-07
>YNL040w [R] KOG2105 Predicted metal-dependent hydrolase contains AlaS
domain
Length = 456
Score = 388 bits (997), Expect = e-108
Identities = 209/445 (46%), Positives = 285/445 (63%), Gaps = 15/445 (3%)
Query: 22 VGSLACQRDSFKAKEFETTVIACDVGKKKNT----FEIELQDTILFPEGGGQPSDSGKFL 77
VG+LACQR+SF F+T V++C+ K K +EIELQDTILFPEGGGQPSDSG
Sbjct: 10 VGALACQRNSFLFDGFKTLVVSCEPTKNKKGEIEGYEIELQDTILFPEGGGQPSDSGFLK 69
Query: 78 LKTG-------ASIPVSSVFRRGLHAVHITDRDVPVGAKVSVEIDWDKRLDYMQQHTGQH 130
+ G I VS V R GLHA H + + G V V +D KR+DYMQQHTGQH
Sbjct: 70 IVEGNRNSSKIEKILVSHVSRFGLHAKHHVNDYIEPGTTVEVAVDEQKRMDYMQQHTGQH 129
Query: 131 LVSAILEQKWGLKTLSWSMGGIPSEKKPKIEPYDLFNYLEINRRLTSEEVKELSDTVTEY 190
L+SAILE+ + + T+SWSMGGI ++KKP +EP D FNY+E+NR+LT +E+ +SD + +
Sbjct: 130 LLSAILERNYKVDTVSWSMGGIITKKKPVLEPSDYFNYIELNRKLTLDEITNVSDEINQL 189
Query: 191 ITVNPKTITIFEG-DPESQGEISTHKVPDDYDLSKGVLRVVHIEDLDKNPCCGTHLQTTS 249
I P+ I + E E+ E+ST K+PDDYDLSKGVLR +HI D+D NPCCGTHL+ TS
Sbjct: 190 IINFPQEIIVEERIGEETVDEVSTSKIPDDYDLSKGVLRTIHIGDIDSNPCCGTHLKCTS 249
Query: 250 QISSVLILPSQSSVRGTNSRLFFMCGDRVRRYALFSNEVISNTKKLLSCSEDQIEHKCDM 309
QI S+LIL +QS+VRG+NSRL+FMCG RV YA N+++ ++K LLSCSE QI K
Sbjct: 250 QIGSILILSNQSAVRGSNSRLYFMCGKRVSLYAKSVNKILLDSKNLLSCSETQISEKITR 309
Query: 310 LLKNMQKTTKREQFWIKELAGFCSKSLITDLKENSK--AHFIRDEYGTLEFLTQLYNENN 367
K +Q+ KREQ+WIK LA S+ L+ LK + K A+F+ +EYGTLE L Q++ E +
Sbjct: 310 QTKQIQQLNKREQYWIKRLARTASEELMNTLKASGKKRAYFMEEEYGTLELLLQIHKEVS 369
Query: 368 QLIAGSSLEDYCYVLAGREKASGGGAIIIVSDSSNRIQEVSDKLKSVVSQLXXXXXXXXX 427
+ + E Y +L G E+ + G+++I+S+S +I ++ L S++ L
Sbjct: 370 NFLKDDT-EGYEIILCGYERQTNTGSLLILSESGEKIANLAANLGSILQNLKGGGGKKGG 428
Query: 428 XXXXXVALYKASEFEGLQHYLDSEF 452
+ +EF L YL +F
Sbjct: 429 KWQGKITSISNAEFAALSDYLSHDF 453
>Hs13376886_2 [R] KOG2105 Predicted metal-dependent hydrolase contains AlaS
domain
Length = 428
Score = 159 bits (401), Expect = 1e-38
Identities = 124/401 (30%), Positives = 202/401 (49%), Gaps = 61/401 (15%)
Query: 36 EFETTVIAC---------DVGKKK--NTFEIELQDTILFPEGGGQPSDSGKFLLKTGASI 84
+F TTV++C GKK+ + F++ L+DT+LFPEGGGQP D G T I
Sbjct: 29 DFTTTVVSCCPAELQTEGSNGKKEVLSGFQVVLEDTVLFPEGGGQPDDRG-----TINDI 83
Query: 85 PVSSVFRRGLHAVHITDRDVPVGAKVSVEIDWDKRLDYMQQHTGQHLVSAILEQKWGLKT 144
V V RRG A H T + G++V V +DW++R D+MQQH+GQHL++A+ + + LKT
Sbjct: 84 SVLRVTRRGEQADHFTQTPLDPGSQVLVRVDWERRFDHMQQHSGQHLITAVADHLFKLKT 143
Query: 145 LSWSMGGIPSEKKPKIEPYDLFNYLEINR-RLTSEEVKELSDTVTEYI----TVNPKTIT 199
SW +G S +E++ +T+E+V + +V E I VN + ++
Sbjct: 144 TSWELGRFRSA-------------IELDTPSMTAEQVAAIEQSVNEKIRDRLPVNVRELS 190
Query: 200 IFEGDPESQGEISTHKVPDDYDLSKGVLRVVHIEDLDKNPCCGTHLQTTSQISSVLILPS 259
+ DPE + ++S +PDD+ G +RVV+IE +D N CCGTH+ S + + IL +
Sbjct: 191 L--DDPEVE-QVSGRGLPDDH---AGPIRVVNIEGVDSNMCCGTHVSNLSDLQVIKILGT 244
Query: 260 QSSVRGTNSRLFFMCGDRVRRYALFSNEVISNTKKLLSC-SEDQIEHKCDMLLKNMQKTT 318
+ + + L F+ G+RV ++ S+ LL C +ED +E +K +Q +T
Sbjct: 245 EKG-KKNRTNLIFLSGNRVLKWMERSHGTEKALTALLKCGAEDHVE-----AVKKLQNST 298
Query: 319 KREQ----FWIKELAGFCSKSLITDLKENSKAHFIRDEYGTLEFLTQLYNENNQLIAGSS 374
K Q +++LA + SL R E G EF+ + NE S
Sbjct: 299 KILQKNNLNLLRDLAVHIAHSLRNSPDWGGVVILHRKE-GDSEFMNIIANEI------GS 351
Query: 375 LEDYCYVLAGREKASGGGAIIIVSDSSNRIQEVSDKLKSVV 415
E ++ G EK GG + +++ ++ + ++ V+
Sbjct: 352 EETLLFLTVGDEK---GGGLFLLAGPPASVETLGPRVAEVL 389
>7290417 [R] KOG2105 Predicted metal-dependent hydrolase contains AlaS
domain
Length = 297
Score = 128 bits (322), Expect = 2e-29
Identities = 81/246 (32%), Positives = 126/246 (50%), Gaps = 34/246 (13%)
Query: 27 CQRDSFKAKEFETTVIACDVG-----------KKKNTFEIELQDTILFPEGGGQPSDSGK 75
CQ DSF KEF+T +++ + +K F + +DTILFPEGGGQP D G
Sbjct: 5 CQEDSF-LKEFKTKIVSSEFATLDWTDPSGKVEKLKGFNVICEDTILFPEGGGQPCDYG- 62
Query: 76 FLLKTGASIPVSSVFRRGLHAVHITDRDVPV--GAKVSVEIDWDKRLDYMQQHTGQHLVS 133
T PV +V R+G AVH + A+V + +D+ +RLD+MQQH+GQHL++
Sbjct: 63 ----TLGGFPVKNVQRKGSTAVHFVESPTSFEQDAEVLLTLDYQRRLDHMQQHSGQHLIT 118
Query: 134 AILEQKWGLKTLSWSMGGIPSEKKPKIEPYDLFNYLEINR-RLTSEEVKELSDTVTEYIT 192
A+ ++++ T SWS+G S Y++++ L S E +L + +
Sbjct: 119 ALFDREFKYDTTSWSLGSTVS-------------YIQLSTPHLISRESLDLIERQANDLI 165
Query: 193 VNPKTITIFEGDPESQGEISTHKVPDDYDLS-KGVLRVVHIEDLDKNPCCGTHLQTTSQI 251
+ +T+ DPE E + P +G+ RVV IE ++ N CCGTH+ SQ+
Sbjct: 166 REGREVTVLLVDPEVAQEFQDARAPRGLPKDHEGLARVVRIEGIESNMCCGTHVTNLSQL 225
Query: 252 SSVLIL 257
+ +L
Sbjct: 226 QCIKLL 231
>Hs22064738 [R] KOG2105 Predicted metal-dependent hydrolase contains AlaS
domain
Length = 307
Score = 94.0 bits (232), Expect = 4e-19
Identities = 84/303 (27%), Positives = 146/303 (47%), Gaps = 45/303 (14%)
Query: 123 MQQHTGQHLVSAILEQKWGLKTLSWSMGGIPSEKKPKIEPYDLFNYLEINR-RLTSEEVK 181
MQQH+GQHL++A+ + + LKT SW +G S +E++ +T+E+V
Sbjct: 1 MQQHSGQHLITAVADHLFKLKTTSWELGRFRSA-------------IELDTPSMTAEQVA 47
Query: 182 ELSDTVTEYIT----VNPKTITIFEGDPESQGEISTHKVPDDYDLSKGVLRVVHIEDLDK 237
+ +V E I VN + +++ DPE + ++S +PDD+ G +RVV+IE +D
Sbjct: 48 AIEQSVNEKIRDRLPVNVRELSL--DDPEVE-QVSGRGLPDDH---AGPIRVVNIEGVDS 101
Query: 238 NPCCGTHLQTTSQISSVLILPSQSSVRGTNSRLFFMCGDRVRRYALFSNEVISNTKKLLS 297
N CCGTH+ S + + IL ++ + + L F+ G+RV ++ S+ LL
Sbjct: 102 NMCCGTHVSNLSDLQVIKILGTEKG-KKNRTNLIFLSGNRVLKWMERSHGTEKALTALLK 160
Query: 298 C-SEDQIEHKCDMLLKNMQKTTKREQ----FWIKELAGFCSKSLITDLKENSKAHFIRDE 352
C +ED +E +K +Q +TK Q +++LA + SL R E
Sbjct: 161 CGAEDHVE-----AVKKLQNSTKILQKNNLNLLRDLAVHIAHSLRNSPDWGGVVILHRKE 215
Query: 353 YGTLEFLTQLYNENNQLIAGSSLEDYCYVLAGREKASGGGAIIIVSDSSNRIQEVSDKLK 412
G EF+ + NE S E ++ G EK GG + +++ ++ + ++
Sbjct: 216 -GDSEFMNIIANEI------GSEETLLFLTVGDEK---GGGLFLLAGPPASVETLGPRVA 265
Query: 413 SVV 415
V+
Sbjct: 266 EVL 268
>Hs20555071 [J] KOG0188 Alanyl-tRNA synthetase
Length = 985
Score = 53.9 bits (128), Expect = 5e-07
Identities = 67/273 (24%), Positives = 112/273 (40%), Gaps = 40/273 (14%)
Query: 57 LQDTILFPEGGGQPSDSGKFLLKTGAS---IPVSSVFRRGLHAVH--ITDRDVPVGAKVS 111
L T + E GGQ SD G +L++ G PV+ G +H + + +G +V
Sbjct: 559 LDRTNFYAEQGGQASDRG-YLVRAGQEDVLFPVARAQVCGGFILHEAVAPECLRLGDQVQ 617
Query: 112 VEIDWDKRLDYMQQHTGQHLVSAILEQKWGLKTLSWSMGGIPSEKKPKIEPYDLFNYLEI 171
+ +D RL M +HT HL++ L Q G T ++ + P L +
Sbjct: 618 LHVDEAWRLGCMAKHTATHLLNWALRQTLGPGT---------EQQGSHLNPEQLRLDVTT 668
Query: 172 NRRLTSEEVKELSDTVTE--------YITVNPKTITIFEGDPESQGEISTHKVPDDYDLS 223
LT E+++ + +TV E Y+ P +T S E+ PD +
Sbjct: 669 QTPLTPEQLRAVENTVQEAVGQDEAVYMEEVPLALTAQVPGLRSLDEV----YPDPVRVV 724
Query: 224 KGVLRVVHIEDLDKNP--------CCGTHLQTTSQISSVLILPSQSSVRGTNSRLFFMCG 275
+ V H D CCGTHL T + ++I+ + +GT +RL + G
Sbjct: 725 SVGVPVAHALDPASQAALQTSVELCCGTHLLRTGAVGDLVIIGDRQLSKGT-TRLLAVTG 783
Query: 276 DRVRRY----ALFSNEVISNTKKLLSCSEDQIE 304
++ ++ + EV + T++L S D E
Sbjct: 784 EQAQQARELGQSLAQEVKAATERLSLGSRDVAE 816
>7295490 [J] KOG0188 Alanyl-tRNA synthetase
Length = 1012
Score = 53.5 bits (127), Expect = 6e-07
Identities = 72/297 (24%), Positives = 122/297 (40%), Gaps = 48/297 (16%)
Query: 65 EGGGQPSDSGKFLLKTGA------SIPVSSVFRRGLHAVHITDRDVP-------VGAKVS 111
E GGQ SD GK L+ S+ V V VHI P +G +V
Sbjct: 582 ESGGQQSDGGKILVSNHQQPDHPHSLDVIGVKHLNDCVVHICKLSSPTDAFQLAIGDEVE 641
Query: 112 VEIDWDKRLDYMQQHTGQHLVSAILEQKWGLKTLSWSMGGIPSEKKPKIEPYDLFNYLEI 171
+++D +R HT HL++A + + K +++ + S + K+E L
Sbjct: 642 LQVDAQQRQLNTCHHTATHLLNAAIRSLF--KKVTYQVSSSVSSDQCKLELGLL------ 693
Query: 172 NRRLTSEEVKELSDTVTEYI-TVNPKTITIFEG-DPESQGEISTHKVPDDYDLSKGVLRV 229
+R+ +V+ + D + I + P + + + Q +I+ VP + +G LR+
Sbjct: 694 GKRIQKTDVQLIEDLINRVICSAAPVEVQLLSAAEVLEQNDITM--VPGEVYPEQG-LRL 750
Query: 230 VHIEDLD-----KNPCCGTHLQTTSQISSVLILPSQSSVRGTNSRLFFMCGDRVRRYALF 284
V++E + K CCGTH TS++S I+ +++ TN F +
Sbjct: 751 VNVESPELQLSSKELCCGTHATNTSELSCFCIV----NLKQTNRARF--------AFTAV 798
Query: 285 SNEVISNTKKLLSCSEDQIEHKCDMLLKNMQKTTKREQFWIKELAGFCSKSLITDLK 341
+ + N K + + H+ D+L K Q T K EL L TD+K
Sbjct: 799 AGQAAENVLKTAAL----LRHRVDLLEKQFQ-TDKLTNATEAELQTIRHNMLHTDIK 850
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,818,577
Number of Sequences: 60738
Number of extensions: 1112834
Number of successful extensions: 2866
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 2850
Number of HSP's gapped (non-prelim): 6
length of query: 452
length of database: 30,389,216
effective HSP length: 110
effective length of query: 342
effective length of database: 23,708,036
effective search space: 8108148312
effective search space used: 8108148312
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)