ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV0767 good J KOG2648 Translation, ribosomal structure and biogenesis Diphthamide biosynthesis protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV0767 249292 251052 587
(587 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL191w [J] KOG2648 Diphthamide biosynthesis protein 503 e-142
SPBC17D1.02 [J] KOG2648 Diphthamide biosynthesis protein 197 3e-50
At3g59630 [J] KOG2648 Diphthamide biosynthesis protein 137 6e-32
7299937 [J] KOG2648 Diphthamide biosynthesis protein 126 8e-29
CE15608 [J] KOG2648 Diphthamide biosynthesis protein 125 2e-28
Hs21536476 [J] KOG2648 Diphthamide biosynthesis protein 100 6e-21
HsM4557533 [J] KOG2648 Diphthamide biosynthesis protein 90 1e-17
SPBC3B8.05 [J] KOG2648 Diphthamide biosynthesis protein 76 2e-13
CE17401 [J] KOG2648 Diphthamide biosynthesis protein 74 5e-13
>YKL191w [J] KOG2648 Diphthamide biosynthesis protein
Length = 534
Score = 503 bits (1294), Expect = e-142
Identities = 275/581 (47%), Positives = 365/581 (62%), Gaps = 65/581 (11%)
Query: 7 VAPSLSTAQTEDTFEFQSYGETEHSRSYLGSDVTKDNLVELVSAYYSVPELIQYFEEHPQ 66
VAP+LST Q++ F+ E + S SYLG D L++L+SAYY+V L+ Y E+HP+
Sbjct: 3 VAPALSTTQSDVAFQKVETHEIDRS-SYLGPCYNSDELMQLISAYYNVEPLVGYLEQHPE 61
Query: 67 YKKITLQFPDELVLDSSIVVQLMQQELVKAEDETSQSFNTIDTDEVLHNEKXXXXXXXXX 126
Y+ +TLQFPD+L+ DSS++V+L+Q +
Sbjct: 62 YQNVTLQFPDDLIKDSSLIVRLLQSKFPHG------------------------------ 91
Query: 127 XXXKIDKTETKRKVWILADTSYSSCCVDEVASEHVKGDIVVHFGDACMNAVQKLPVVYSL 186
K K W+LADT+YS+CCVDEVA+EHV ++VVHFGDAC+NA+Q LPVVYS
Sbjct: 92 ----------KIKFWVLADTAYSACCVDEVAAEHVHAEVVVHFGDACLNAIQNLPVVYSF 141
Query: 187 GRPVLDLDLVVSQFKLKYAAKDQKICLMADAPHSMHMKSIYDILHDKEGYKNVVYSDINQ 246
G P LDL LVV F+ + KICLMA+AP S H+ +Y+IL Y N++YS +N
Sbjct: 142 GTPFLDLALVVENFQRAFPDLSSKICLMANAPFSKHLSQLYNILKGDLHYTNIIYSQVNT 201
Query: 247 DMLQSD-THIVGYNNAVEHDERL----RKCVTCGNRNIYAEVDVSELNEYDLFHVTIPKD 301
++ I+ + E +++ + V G + + D +Y LFH+T P+D
Sbjct: 202 SAVEEKFVTILDTFHVPEDVDQVGVFEKNSVLFGQHD---KADNISPEDYHLFHLTTPQD 258
Query: 302 PHLLYLTTKFQSVTLYDPSSGMLNEGPFPSMMKRYKFMHMARTAGTIGILVNTLSLRNTK 361
P LLYL+T FQSV ++DP+ + GPFPS+M+RYK+MH+ARTAG IGILVNTLSLRNT+
Sbjct: 259 PRLLYLSTVFQSVHIFDPALPGMVTGPFPSLMRRYKYMHVARTAGCIGILVNTLSLRNTR 318
Query: 362 ETMNKLTKLLKENGKKHYLFVVGKPNVAKLANFEPIDIWCILGCGQGGIVLDQYNEFYKP 421
ET+N+L KL+K KKHYLFVVGKPNVAKLANFE IDIWCILGC Q GI++DQ+NEFYKP
Sbjct: 319 ETINELVKLIKTREKKHYLFVVGKPNVAKLANFEDIDIWCILGCSQSGIIVDQFNEFYKP 378
Query: 422 IITPYELLMALSDEVTWTGQWITDFKSIINQIENEVNDSDDMEQDILSSGTECRSDEDEA 481
IITPYEL +ALS+EVTWTG+W+ DF+ I++IE + QD +S+ T SDE
Sbjct: 379 IITPYELNLALSEEVTWTGKWVVDFRDAIDEIEQNLGG-----QDTISAST--TSDE--- 428
Query: 482 PEFNAVTGKYVSTSRPLRQIARLEIETPVEEVRASDSTELVKQFSQTVA-IRNTVSTSAA 540
PEF+ V G+Y STSRPLR + LE+E ++ DS +L + + + A I+ TVSTSA+
Sbjct: 429 PEFDVVRGRYTSTSRPLRALTHLELEAADDD----DSKQLTTRHTASGAVIKGTVSTSAS 484
Query: 541 FLQTRHWTGLGSDYKXXXXXXXXXXXXXXXXXXVARSYQFD 581
LQ R W GLGSD+ VAR Y FD
Sbjct: 485 ALQNRSWKGLGSDF-DSTEVDNTGADIEEGISGVARGYGFD 524
>SPBC17D1.02 [J] KOG2648 Diphthamide biosynthesis protein
Length = 503
Score = 197 bits (502), Expect = 3e-50
Identities = 146/501 (29%), Positives = 236/501 (46%), Gaps = 87/501 (17%)
Query: 61 FEEHPQYKKITLQFPDELVLDSSIVVQLMQQELVKAEDETSQSFNTIDTDEVLHNEKXXX 120
F + Y + LQFPDE + DS V ++ LV+A
Sbjct: 45 FIKSGNYNSVALQFPDEHLADSGKVASILTN-LVEAN----------------------- 80
Query: 121 XXXXXXXXXKIDKTETKRKVWILADTSYSSCCVDEVASEHVKGDIVVHFGDACMNAVQKL 180
V ILADT+Y SCCVDEVA+EH+ D +VH+G AC++ +L
Sbjct: 81 -------------------VQILADTNYGSCCVDEVAAEHMSADAIVHYGRACLSPTSRL 121
Query: 181 PVVYSLGRPVLDLDLVVSQFKLKYAAKDQKICLMADAPHSMHMKSIYDILHDKEGYKNVV 240
PV+Y GR ++L + + DQ I L++D SI L GY+NV
Sbjct: 122 PVLYVFGRLPINLHKLEKCLTIPL---DQNILLVSDTRWYYAQDSILKSLKTL-GYQNVY 177
Query: 241 YSDI------NQDMLQSDTHIVGYNNAVEHDERLRKCVTCGNRNIYAEVDVSELNEYDLF 294
S + N + + I G ++ L+ +Y D L+ +
Sbjct: 178 ESHLKERIEPNLEEASTSYTIPGRTYSLPKSLSLQDMTL-----LYIGPDSPTLSSILMS 232
Query: 295 HVTIPKDPHLLYLTTKFQSVTLYDP-SSGMLNEGPFPS--MMKRYKFMHMARTAGTIGIL 351
H + L +F S +DP S+ ++ E F + +RY + R AG IGI+
Sbjct: 233 HYS---------LVNQFLS---FDPLSNKIVEESSFTGAKLRRRYALVQRCRDAGVIGIV 280
Query: 352 VNTLSLRNTKETMNKLTKLLKENGKKHYLFVVGKPNVAKLANFEPIDIWCILGCGQGGIV 411
+ TL + +N+L K++ GKK Y+ VGK N AKLANF+ I+ + ++ CG+ ++
Sbjct: 281 IGTLGVHRYLHVLNQLRKMILNAGKKPYMLAVGKLNPAKLANFQEIECFVLIACGENSLI 340
Query: 412 LDQYNEFYKPIITPYELLMALSDEVTWTGQWITDFKSIINQIENEVNDSDDMEQDILSSG 471
EFY+PI+TP+EL+ ALS +++W +I F ++ E + +
Sbjct: 341 --DSKEFYRPIVTPFELVKALSSDMSWNNDFILSFDEVLKLSEGKQS----------KEP 388
Query: 472 TECRSDEDEAPEFNAVTGKYVSTSRPLRQIARLEIETPVEEVRASDSTELVKQFSQTVAI 531
+E ++E P F+ +TGK+V+ S P+R + + +ET + S S + K+ +++A+
Sbjct: 389 SEVLTEESAEPHFSLITGKFVN-STPMRHL-DVTLETADAKNNDSSSASIEKRGMRSLAV 446
Query: 532 RNTVSTSAAFLQTRHWTGLGS 552
S +AAFLQ++ W+GL S
Sbjct: 447 NGVYSPAAAFLQSKSWSGLDS 467
>At3g59630 [J] KOG2648 Diphthamide biosynthesis protein
Length = 491
Score = 137 bits (344), Expect = 6e-32
Identities = 118/474 (24%), Positives = 203/474 (41%), Gaps = 65/474 (13%)
Query: 67 YKKITLQFPDELVLDSSIVVQLMQQELVKAEDETSQSFNTIDTDEVLHNEKXXXXXXXXX 126
+ +I LQFPDEL+ DS+ VV ++ + D + F
Sbjct: 23 FTRIALQFPDELLKDSTKVVSALKSKTRLLTDREVRFF---------------------- 60
Query: 127 XXXKIDKTETKRKVWILADTSYSSCCVDEVASEHVKGDIVVHFGDACMNAVQKLPVVYSL 186
++ADT+Y SCC+DEV + H+ + VVH+G C++ LP +
Sbjct: 61 ---------------VMADTTYGSCCIDEVGALHIDSECVVHYGQTCLSPTSVLPAFFVF 105
Query: 187 GRPVLDLDLVVSQFKLKYAAK-DQKICLMADAPHSMHMKSIYDIL-----HDKEGYKNVV 240
G+ +++ V + YA+K D+ I ++ ++ + I + L + NV+
Sbjct: 106 GKASINVSSCVKHL-IDYASKSDKPIMILYGLEYAHVIPQIREELGLSKTDSQLSVANVL 164
Query: 241 YSDIN--QDMLQSDTHIVGYNNAVEHDERLRKCVTCGNRNIYAEVDVSELNEYDLFHVTI 298
S I+ +D +S H Y+ + D + + S++ +Y LF +
Sbjct: 165 CSFISPSKDPRESMEHPRPYSESDSSDSLSSSRSYRLGGLTWDLPEGSKIEDYLLFWIGS 224
Query: 299 PKDPHL-LYLTTKFQSVTLYDPSSGMLNEGPFPS---MMKRYKFMHMARTAGTIGILVNT 354
+ LT + YD L + + +RY + A+ A IGILV T
Sbjct: 225 DSSAFANVVLTFNGCDIVRYDAEEDSLVTEFYQQRRILKRRYYLVEKAKDANIIGILVGT 284
Query: 355 LSLRNTKETMNKLTKLLKENGKKHYLFVVGKPNVAKLANFEPIDIWCILGCGQGGIVLDQ 414
L + ++ + L+ GKK Y+ +G+PN AKLANF D++ + C Q ++
Sbjct: 285 LGVAGYLHMIHHMQALISAAGKKSYILAMGRPNPAKLANFPECDVFIYISCAQTALL--D 342
Query: 415 YNEFYKPIITPYELLMALSDEVTWTGQWITDFKSIINQIENEVN---DSDDMEQDILSSG 471
EF P+ITP+E +A S WTG ++ F+ +IN +++E S++ G
Sbjct: 343 SKEFMSPVITPFEANLAFSRGSEWTGAYLMHFQDVINSVKSESEAHIGSEEPRFSFFQGG 402
Query: 472 -TECRSDEDEAPEFNAVTGKYVSTSRPLRQIARLEIETPVEEVRASDSTELVKQ 524
E D+A TG+ ++ L Q A + ++R +D L KQ
Sbjct: 403 YVEDHKTNDQAKNGEEDTGETMT----LVQAAEKAL-----QLRGNDHNSLTKQ 447
>7299937 [J] KOG2648 Diphthamide biosynthesis protein
Length = 469
Score = 126 bits (317), Expect = 8e-29
Identities = 109/397 (27%), Positives = 179/397 (44%), Gaps = 88/397 (22%)
Query: 67 YKKITLQFPDELVLDSSIVVQLMQQELVKAEDETSQSFNTIDTDEVLHNEKXXXXXXXXX 126
YK++ LQFPD+ L S + + +EL+ ED
Sbjct: 52 YKRVCLQFPDDY-LPHSNAISVGLKELLAPEDV--------------------------- 83
Query: 127 XXXKIDKTETKRKVWILADTSYSSCCVDEVASEHVKGDIVVHFGDACMNAVQKLPVVYSL 186
KV+ILADT+Y SCCVDE+A+ HV D V+HFG+AC + +LPV+Y
Sbjct: 84 ------------KVFILADTTYGSCCVDEIAAAHVDADSVIHFGNACRSRAARLPVLYLY 131
Query: 187 GRPVLDLDLVVSQFK-LKYAAKDQKICLMADAPHSMHM------KSIYDILHDKEGYKNV 239
LD+++++S+ + L+ + D+++C+ D + H+ K + +IL KE
Sbjct: 132 PELPLDVNVLLSKLQGLRAESIDRQVCVYLDIGY-QHVYGEKLKKQLSEILEPKE----- 185
Query: 240 VYSDINQDMLQSDTHIVGYNNAVEHDERL-RKCVTCGNRNIYAEVDVSELNEYDLFHVTI 298
+L+ I + L R C+ G N + +T
Sbjct: 186 -------LLLELFPPIEAESTTTRETTNLERICIFIGADN----------QRFANLSLTA 228
Query: 299 PKDPHLLYLTTKFQSVTLYDPSSGML-NEGPFPSMMKRYKFMHM--ARTAGTIGILVNTL 355
P +YD SS L ++ P + R ++ H+ + A T+G++V TL
Sbjct: 229 PAAVQW----------HIYDGSSDSLSSKNPLTAQFIRRRYFHIEKCKDAQTLGLIVATL 278
Query: 356 SLRNTKETMNKLTKLLKENGKKHYLFVVGKPNVAKLANFEPIDIWCILGCGQGGIVLDQY 415
S + + +L + K G K L VG+ N AKLANF ID + ++GC +
Sbjct: 279 SAEGYLDVVTRLQTMAKGRGIKTQLISVGRINPAKLANFLEIDCFVLIGCPFNNMY--DS 336
Query: 416 NEFYKPIITPYELLMALSD--EVTWTGQWITDFKSII 450
E+YKPI++ +E MAL+ + + ++TDFK ++
Sbjct: 337 KEYYKPIVSVFEAEMALNPAWHMRYPEAYVTDFKQLL 373
>CE15608 [J] KOG2648 Diphthamide biosynthesis protein
Length = 476
Score = 125 bits (313), Expect = 2e-28
Identities = 98/356 (27%), Positives = 181/356 (50%), Gaps = 41/356 (11%)
Query: 133 KTETKRKVWILADTSYSSCCVDEVASEHVKGDIVVHFGDACMNA-VQKLPVVYSLGRPVL 191
++E+ +K ++LADTSY SCCVDEVA+ H +VHFG+AC +A K+ V Y LG
Sbjct: 83 RSESAKKTFVLADTSYRSCCVDEVAAAHADCTALVHFGEACHSAPTDKIDVKYVLG---- 138
Query: 192 DLDLVVSQFKLKYAAKDQKICLMADAPHSMHMKSIYD--ILHDKEGYKNVVYSDINQDML 249
+ + + QF++++ +AD + H+ + D H++E V+ +++L
Sbjct: 139 SMPIFIDQFRMEFQN-------VADQLTAEHIVLLMDSCFSHEQEKVAAVI-----KEVL 186
Query: 250 QSDTHIVGYNNAVEHDERLRKCVTCGNRNIYAEVDVSEL----NEYDLFHVTIPKDPHLL 305
+ H+ + + ++ L++ +NIY ++ DL P P L
Sbjct: 187 PGNRHV--ECSLLPSEDVLKQ----NRQNIYLGREIPSCLRNNQPTDLIFCGFPNSPLLP 240
Query: 306 YLTTKFQS---VTLYDPSSGMLNEGPFPS---MMKRYKFMHMARTAGTIGILVNTLSLRN 359
+ S V+ ++P + + S + KR + + A T+G++V ++ +
Sbjct: 241 IWLLSYPSCSTVSHFNPINKTIQHESTRSSRLLRKRLFLVEKLKDADTVGLVVGSVGVDK 300
Query: 360 TKETMNKLTKLLKENGKKHYLFVVGKPNVAKLANFE-PIDIWCILGCGQGGIVLDQYNEF 418
+E + ++ ++ K+ GKK Y+ VGK NV KL+NF ID++ +L C G+VLD +++
Sbjct: 301 HREAVKRMREMCKKAGKKIYVISVGKINVPKLSNFSTDIDVFVLLSC-PFGVVLDS-SDY 358
Query: 419 YKPIITPYELLMALSDEVTWTGQ--WITDFKSII-NQIENEVNDSDDMEQDILSSG 471
++P+++ +E +AL+ TW W +F + + ++IE EV D L SG
Sbjct: 359 FRPVVSYFEAEIALNPAKTWAADFGWSAEFAAFLEDKIETEVPDDKAAGDFSLISG 414
>Hs21536476 [J] KOG2648 Diphthamide biosynthesis protein
Length = 489
Score = 100 bits (249), Expect = 6e-21
Identities = 81/307 (26%), Positives = 140/307 (45%), Gaps = 29/307 (9%)
Query: 136 TKRKVWILADTSYSSCCVDEVASEHVKGDIVVHFGDACMNA-VQKLPVVYSLGRPVLDLD 194
T K++IL DT+Y SCCVD + +E ++HFG AC++ + LPV + L + + L+
Sbjct: 73 TGSKMFILGDTAYGSCCVDVLGAEQAGAQALIHFGPACLSPPARPLPVAFVLRQRSVALE 132
Query: 195 LVVSQFKLKYAAKDQKICLMADAPHSMHMKSIYDILHDKEGYKNVVYSDINQDMLQSDTH 254
L V F+ + + L+++ + ++++ +L + Y D+ ++ S
Sbjct: 133 LCVKAFEAQNPDPKAPVVLLSEPACAHALEALATLLRPR-------YLDL---LVSSPAF 182
Query: 255 IVGYNNAVEHDERLRKCVTCGNRNIYAEVDVSELNEYDLFHVTIPK-------DPHLLYL 307
+ L + G R + L EY F+V K DP L L
Sbjct: 183 PQPVGSLSPEPMPLERF---GRR--FPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRL 237
Query: 308 TTKF---QSVTLYDPSSGML-NEGPFPSMMK--RYKFMHMARTAGTIGILVNTLSLRNTK 361
+ Q + P +G +EG ++ R + AR A +G+L TL + +
Sbjct: 238 LLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARDARVVGLLAGTLGVAQHR 297
Query: 362 ETMNKLTKLLKENGKKHYLFVVGKPNVAKLANFEPIDIWCILGCGQGGIVLDQYNEFYKP 421
E + L L + GK+ Y+ +G+P AKLANF +D++ +L C G + F++P
Sbjct: 298 EALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQLSGSFFQP 357
Query: 422 IITPYEL 428
I+ P EL
Sbjct: 358 ILAPCEL 364
>HsM4557533 [J] KOG2648 Diphthamide biosynthesis protein
Length = 489
Score = 89.7 bits (221), Expect = 1e-17
Identities = 82/308 (26%), Positives = 131/308 (41%), Gaps = 31/308 (10%)
Query: 136 TKRKVWILADTSYSSCCVDEVASEHVKGDIVVHFGDACMNAVQK-LPVVYSLGRPVLDLD 194
T K++IL DT+Y SCCVD + +E ++HFG AC++ + LPV + L L
Sbjct: 73 TGSKMFILGDTAYGSCCVDVLGAEQAGXQALIHFGPACLSPPXRPLPVAFVLSSTFCGLG 132
Query: 195 LVVSQFKLKYAAKDQKICLMADAPHSMH-MKSIYDILHDKEGYKNVVYSDINQDMLQSDT 253
+ Q + Q C A P H ++++ +L + Y D+
Sbjct: 133 -TLCQDLWGPKPRXQSACGAAGEPACAHALEALAXLLRPR-------YLDLLVSSPAFPQ 184
Query: 254 HIVGYNNAVEHDERLRKCVTCGNRNIYAEVDVSELNEYDLFHVTIPK-------DPHLLY 306
+ + ER G R + L EY F+V K DP L
Sbjct: 185 PVGSLSPEPMPXERF------GRR--FPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSR 236
Query: 307 LTTKF---QSVTLYDPSSGML-NEGPFPSMMK--RYKFMHMARTAGTIGILVNTLSLRNT 360
L + Q + P +G +EG ++ R + AR A +G+L T +
Sbjct: 237 LLLGWAPGQPFSSCCPDTGKTQDEGARAGGLRARRRYXVERARDARVVGLLAGTXGVAQH 296
Query: 361 KETMNKLTKLLKENGKKHYLFVVGKPNVAKLANFEPIDIWCILGCGQGGIVLDQYNEFYK 420
+E + L L + GK+ Y+ +G+P AKLANF +D++ +L C G + F++
Sbjct: 297 REALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQLSGSFFQ 356
Query: 421 PIITPYEL 428
PI+ P EL
Sbjct: 357 PILAPCEL 364
>SPBC3B8.05 [J] KOG2648 Diphthamide biosynthesis protein
Length = 436
Score = 75.9 bits (185), Expect = 2e-13
Identities = 74/312 (23%), Positives = 129/312 (40%), Gaps = 33/312 (10%)
Query: 142 ILADTSYSSCCVDEVASEHVKGDIVVHFGDACMNAVQKLP--VVYSLGRPVLDLDLVVSQ 199
++ D +Y +CC+D+ + + D +VH+G +C+ V + P V+Y +DL VVS
Sbjct: 121 VMGDVTYGACCIDDFTARALDCDFLVHYGHSCLIPVDQTPIKVLYVFVDIKIDLQHVVSS 180
Query: 200 FKLKYAAKDQKICLMADAPHSMHMKSIYDILH--DKEGYKNVVYSDINQDMLQSDTHIVG 257
K + + ++ L+ + SI D L D++G K Y I Q S +G
Sbjct: 181 LKHNLPS-NSRLALVGTIQFVGSLNSIKDALQIQDEDG-KGGFYVVIPQAKPLSPGEALG 238
Query: 258 YNNAVEHDERLRKCVTCGNRNIYAEVDVSELNEYDLFHVTIPKDPHLLYLTTKFQSVTLY 317
+ + + G+ + E + + + Y
Sbjct: 239 CTSPYIEKGSVDALIYIGDGRFHLESVMIANPDLPAYR---------------------Y 277
Query: 318 DPSSGMLN-EGPFPSMMK--RYKFMHMARTAGTIGILVNTLSLRNTKETMNKLTKLLKEN 374
DP S L+ E MK RY + ARTA G++ TL + + + + L L++N
Sbjct: 278 DPYSHKLSIESYAHEEMKSIRYSAVEKARTAKKFGLIQGTLGRQGSPKVLENLKNTLRKN 337
Query: 375 GKKHYLFVVGKPNVAKLANFEPIDIWCILGCGQGGIVLDQYNEFYKPIITPYELLMALSD 434
K ++ + ++L F ID W + C + + +D F P++TPYE A +
Sbjct: 338 NKDFVCVLMSEIFPSRLGQFSDIDAWIQVACPR--LSIDWGYAFPAPLLTPYEASAAF-N 394
Query: 435 EVTWTGQWITDF 446
V W + DF
Sbjct: 395 VVPWKEVYPMDF 406
>CE17401 [J] KOG2648 Diphthamide biosynthesis protein
Length = 396
Score = 74.3 bits (181), Expect = 5e-13
Identities = 66/310 (21%), Positives = 135/310 (43%), Gaps = 28/310 (9%)
Query: 142 ILADTSYSSCCVDEVASEHVKGDIVVHFGDACMNAVQK---LPVVYSLGRPVLDLDLVVS 198
I+ D +Y +CCVD+ ++ + D++VH+G +C+ +Q + ++Y ++L ++
Sbjct: 80 IMGDVTYGACCVDDYTAKSMGCDLLVHYGHSCLVPIQNTDGIAMLYVFVNIHINLSHLID 139
Query: 199 QFKLKYAAKDQKICLMADAPHSMHMKSIYDILHDKEGYKNVVYSDINQDMLQSDTHIVGY 258
K + K ++ +++ ++++ + + + + DI Q S ++G
Sbjct: 140 CVKENFQGK--RLVVVSTVQFIPSLQTLRTTFNKDD---SSIRIDIPQCKPLSPGEVLGC 194
Query: 259 NNAVEHDERLRKCVTCGNRNIYAEVDVSELNEYDLFHVTIPKDPHLLYLTTKFQSVTLYD 318
+ + V G+ + E + E + F DP+ LT +F YD
Sbjct: 195 TSPRLDASKYDAIVYLGDGRFHLESIMIHNPEIEAFQY----DPYSRKLTREF-----YD 245
Query: 319 PSSGMLNEGPFPSMMKRYKFMHMARTAGTIGILVNTLSLRNTKETMNKLTKLLKENGKKH 378
N R + +AR T G++ TL + + + +L L+ GKK
Sbjct: 246 HDLMRKN---------RIGSIEIARKCTTFGLIQGTLGRQGNLKVVEELEAQLERKGKKF 296
Query: 379 YLFVVGKPNVAKLANFEPIDIWCILGCGQGGIVLDQYNEFYKPIITPYELLMALSDEVTW 438
++ + KLA F +D W + C + + +D +F KP++ P+EL +AL +
Sbjct: 297 LRVLLSEIFPEKLAMFPEVDCWVQVACPR--LSIDWGTQFPKPLLYPFELAVALDNISVP 354
Query: 439 TGQWITDFKS 448
+ W D+ S
Sbjct: 355 SDHWPMDYYS 364
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.132 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,032,677
Number of Sequences: 60738
Number of extensions: 1428269
Number of successful extensions: 4250
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 4216
Number of HSP's gapped (non-prelim): 23
length of query: 587
length of database: 30,389,216
effective HSP length: 112
effective length of query: 475
effective length of database: 23,586,560
effective search space: 11203616000
effective search space used: 11203616000
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)