ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV0997 good R KOG0289 General function prediction only mRNA splicing factor
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV0997 328358 329830 491
(491 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLL036c [R] KOG0289 mRNA splicing factor 178 2e-44
SPAC29A4.08c [R] KOG0289 mRNA splicing factor 114 4e-25
At2g33340 [R] KOG0289 mRNA splicing factor 103 6e-22
Hs7657381 [R] KOG0289 mRNA splicing factor 102 1e-21
CE02043 [R] KOG0289 mRNA splicing factor 102 1e-21
7302603 [R] KOG0289 mRNA splicing factor 81 4e-15
At1g04510 [R] KOG0289 mRNA splicing factor 74 4e-13
>YLL036c [R] KOG0289 mRNA splicing factor
Length = 503
Score = 178 bits (451), Expect = 2e-44
Identities = 135/506 (26%), Positives = 226/506 (43%), Gaps = 75/506 (14%)
Query: 1 MFCAISGKPPIKAVLSPNSKCIFEQHLIEQYIEQKGTDPITDDPLQKTDLVEINATPQQI 60
M CAISGK P + VLSP S+ IFE+ L+EQY++ G DPIT++PL ++VEI + QQ
Sbjct: 1 MLCAISGKVPRRPVLSPKSRTIFEKSLLEQYVKDTGNDPITNEPLSIEEIVEIVPSAQQA 60
Query: 61 XXXXXXXXXTIANNYSIPSLLSTLQKEWDAVMLENFELRKQLDVCKKNLSDTLYRFDXXX 120
T+ NYSIP+LL++LQ EWDA+MLENF+LR LD K LS +Y D
Sbjct: 61 SLTESTNSATLKANYSIPNLLTSLQNEWDAIMLENFKLRSTLDSLTKKLSTVMYERDAAK 120
Query: 121 XXXXXXFVERDQLKQELAELTSAITSSNVENDGTIDKLQNSDQILSDFTSELSTKSQEYV 180
+E+++ ++L + + + E +F L S+++V
Sbjct: 121 LVAAQLLMEKNEDSKDLPKSSQQAVAITRE----------------EFLQGLLQSSRDFV 164
Query: 181 TNSKKSKKKLQHFSNVSVVQSTDFSEIDLISRNQHVNLNERSVVVKDNDKAFVFGPQDSI 240
K K N+ ++Q+ ++S N ++ K+ +K+ + +
Sbjct: 165 ARGKLKAPKWPILKNLELLQAQNYSR------------NIKTFPYKELNKSMYYDKWVCM 212
Query: 241 LDVSQATEYLVEFKD-----------------------------VLYTFTQGLLAAYDLH 271
+ + KD +L + + D
Sbjct: 213 CRCEDGALHFTQLKDSKTITTITTPNPRTGGEHPAIISRGPCNRLLLLYPGNQITILDSK 272
Query: 272 TKSLLYEEVLEGTDGPVIFMAYPHQISDSYFLFVTERGTIYGYEVTKKALFTISQVKLND 331
T +L E ++ + +I+M ++++ YF++ RGTI + + + K +
Sbjct: 273 TNKVLREIEVDSAN-EIIYMYGHNEVNTEYFIWADNRGTIGFQSYEDDSQYIVHSAKSDV 331
Query: 332 SVQFVNYHKDGALLAIGNQNEGVYVINLLAATKDPIKFSTGFPA------KRVDFGLNGY 385
HKD LLA+ + + + V NL P + S+ FP K V F NGY
Sbjct: 332 EYSSGVLHKDSLLLALYSPDGILDVYNL----SSPDQASSRFPVDEEAKIKEVKFADNGY 387
Query: 386 WMFVFGDSDLNVYDLRKDPGTLAMEPLS---FAENIISVELDDTAKGFFV-SMGNSQVQW 441
WM V D + +DLRKD GTLA + F ++ ++DD+ K S ++ +
Sbjct: 388 WMVVECDQTVVCFDLRKDVGTLAYPTYTIPEFKTGTVTYDIDDSGKNMIAYSNESNSLTI 447
Query: 442 YEY---VKGQSFVEKSTLPIETNTAE 464
Y++ K + E+S L ++++TA+
Sbjct: 448 YKFDKKTKNWTKDEESALCLQSDTAD 473
>SPAC29A4.08c [R] KOG0289 mRNA splicing factor
Length = 488
Score = 114 bits (284), Expect = 4e-25
Identities = 121/470 (25%), Positives = 202/470 (42%), Gaps = 47/470 (10%)
Query: 1 MFCAISGKPPIKAVLSPNSKCIFEQHLIEQYIEQKGTDPITDDPLQKTDLVEINATPQQI 60
MFC+ISG+ P + V+S S ++E+ LIEQ I + DP+T DLV + P +
Sbjct: 1 MFCSISGETPKEPVISRVSGNVYEKRLIEQVIRETSKDPVTQQECTLEDLVPVKV-PDFV 59
Query: 61 XXXXXXXXXTIANNYSIPSLLSTLQKEWDAVMLENFELRKQLDVCKKNLSDTLYRFDXXX 120
S+P+LLS Q+EWD+V LE FELR+ L K+ LS LY D
Sbjct: 60 RPRPPSAT-------SLPALLSLFQEEWDSVALEQFELRRNLTETKQELSTALYSLDAAL 112
Query: 121 XXXXXXFVERDQLKQELAELTSAI--TSSNVENDGTIDKLQNSDQILSDFTSELSTKSQE 178
ERD+ ++ LA+ + I SS ++ ++ DQ+ + S + QE
Sbjct: 113 RVISRLTKERDEAREALAKFSDNIGTVSSKTIEVQEVEMGESDDQLKTSLRSTVEKTFQE 172
Query: 179 YVTNSKKSKKKLQHFSNVSVVQ------STDFSEIDLISRNQHVNLNERS--VVVKDNDK 230
+ K++K + + ++ +V Q ST ++ S E S V+ D+
Sbjct: 173 LSSKRKRTKLQPKWATDDAVSQLLQATPSTILENLETESTTSFFPSPENSSFVLCLHKDE 232
Query: 231 AFVFGPQ-DSILDVSQATE----YLVEFKDVLYTFTQGLLAAYDLHTKSLLYEEVLEGT- 284
Q +S L + + + +L K + T ++ ++ S + V E T
Sbjct: 233 LLCLDIQSNSTLKIFEGSALACCWLTSSK--IAVATADAISIFEFPVSSSGLQSVGEPTS 290
Query: 285 ----DGPVIFM-AYPHQISDSYFLFVTERGTIYGYEVTKKALFTISQVKLNDSVQFVNYH 339
D V F+ A+P +L Y + + + + + + + + H
Sbjct: 291 SIPIDEKVNFLQAHP----SGEYLLAASNEKCYIFSLKSQ----VYNITVAQHITSLAVH 342
Query: 340 KDGALLAIGNQNEGVYVINLLAATKDPIKFST-GFPAKRVDFGLNGYWMFVF--GDSDLN 396
DG L G +N G ++ + KF P K + FG NGYW+ V DSD+
Sbjct: 343 PDGNLFVAGLEN-GELRFFETSSGNELTKFGPHSSPVKTLQFGENGYWLVVTTNDDSDIF 401
Query: 397 VYDLRKDPGTLAMEPLSFAENIISVELDDTAKGFFVSMGNSQVQWYEYVK 446
++DLRK ++ + + +V LD T++ VS + + YVK
Sbjct: 402 IWDLRKSE---LVQKIPLQTKVAAVSLDITSQ-LLVSSDGETLYVHIYVK 447
>At2g33340 [R] KOG0289 mRNA splicing factor
Length = 540
Score = 103 bits (257), Expect = 6e-22
Identities = 114/470 (24%), Positives = 206/470 (43%), Gaps = 62/470 (13%)
Query: 1 MFCAISGKPPIKAVLSPNSKCIFEQHLIEQYIEQKGTDPITDDPLQKTDLVEINATPQQI 60
M CAISG+ P++ V+S S +FE+ LIE++I G P+T +PL D+V I
Sbjct: 1 MNCAISGEVPVEPVVSTKSGLLFERRLIERHISDYGKCPVTGEPLTIDDIVPIKT----- 55
Query: 61 XXXXXXXXXTIANNYSIPSLLSTLQKEWDAVMLENFELRKQLDVCKKNLSDTLYRFDXXX 120
+ SIP LL T Q EWD +ML NF L +QL ++ LS LY+ D
Sbjct: 56 ---GEIIKPKTLHTASIPGLLGTFQNEWDGLMLSNFALEQQLHTARQELSHALYQHDSAC 112
Query: 121 XXXXXXFVERDQLKQELAEL-------TSAITSSNVENDG---TIDKLQNSD--QILSDF 168
ERD+ +Q LAE+ A+T++ ++G +D+ D ++
Sbjct: 113 RVIARLKKERDEARQLLAEVERHIPAAPEAVTANAALSNGKRAAVDEELGPDAKKLCPGI 172
Query: 169 TSELSTKSQEYVTNSKKSKKKLQHFSNVSVVQSTDFSEIDLISRNQHVNLNERSVVVKDN 228
++E+ T+ + + +KK Q + Q+ + ID + R ++ + K
Sbjct: 173 SAEIITELTDCNAALSQKRKKRQ------IPQT--LASIDTLERFTQLSSHPLHKTNK-- 222
Query: 229 DKAFVFGPQDSILDVSQATEYL----VEFKDVLYTFTQG-LLAAYDLHTKSLLYEEVLEG 283
P +D+ + + + V+ VL+ G +L+ H+K + + +
Sbjct: 223 -------PGICSMDILHSKDVIATGGVDATAVLFDRPSGQILSTLTGHSKKVTSVKFVGD 275
Query: 284 TDGPVIFMAYPHQI-------SDSYFLFVTERGTIYGYEVTK-KALFTISQVKLNDSVQF 335
+D ++ A ++ ++ YF+ + GT Y+++ L +S N
Sbjct: 276 SD--LVLTASADKVRAVTVHPTNKYFVSASLDGTWCFYDLSSGSCLAQVSDDSKNVDYTA 333
Query: 336 VNYHKDGALLAIGNQNEGVYVINLLAATKDPIKFS--TGFPAKRVDFGLNGYWMFVFGDS 393
+H DG +L G ++ V I + + + KF TG + F NGY++ +
Sbjct: 334 AAFHPDGLILGTGT-SQSVVKIWDVKSQANVAKFDGHTG-EVTAISFSENGYFLATAAED 391
Query: 394 DLNVYDLRKDPGTLAMEPLSFAENIISVELDDTAKGFFVSMGNSQVQWYE 443
+ ++DLRK L + + SVE D + G ++ + S ++ Y+
Sbjct: 392 GVRLWDLRK----LRNFKSFLSADANSVEFDPS--GSYLGIAASDIKVYQ 435
>Hs7657381 [R] KOG0289 mRNA splicing factor
Length = 504
Score = 102 bits (255), Expect = 1e-21
Identities = 108/475 (22%), Positives = 193/475 (39%), Gaps = 50/475 (10%)
Query: 1 MFCAISGKPPIKAVLSPNSKCIFEQHLIEQYIEQKGTDPITDDPLQKTDLVEIN-ATPQQ 59
+ C+IS + P +SP S ++E+ LIE+YI + GTDPI + PL + L++I A P +
Sbjct: 3 LICSISNEVPEHPCVSPVSNHVYERRLIEKYIAENGTDPINNQPLSEEQLIDIKVAHPIR 62
Query: 60 IXXXXXXXXXTIANNYSIPSLLSTLQKEWDAVMLENFELRKQLDVCKKNLSDTLYRFDXX 119
SIP++L LQ EWDAVML +F LR+QL ++ LS LY+ D
Sbjct: 63 PKPPSAT---------SIPAILKALQDEWDAVMLHSFTLRQQLQTTRQELSHALYQHDAA 113
Query: 120 XXXXXXXFVERDQLKQELAEL--------TSAITSSNVENDGTIDKLQNSDQILSDFTSE 171
E ++ LA L A+ SS G + + + L T E
Sbjct: 114 CRVIARLTKEVTAAREALATLKPQAGLIVPQAVPSSQPSVVGAGEPMDLGE--LVGMTPE 171
Query: 172 LSTKSQEYVTNSKKSKKKLQHFSNVSVVQSTDFSEIDLISRNQHVNLNERSV-------- 223
+ K Q+ T +KK +V+ + S+ ++ HV L+ S+
Sbjct: 172 IIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQVA--SHVGLHSASIPGILALDL 229
Query: 224 VVKDNDKAFVFGPQDSILDVSQATEYLV--------EFKDVLYTFTQGLLAAYDLHTKSL 275
D +K G +++ +++E ++ + V++ +Q L+ +
Sbjct: 230 CPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSVVFHPSQDLVFSASPDATIR 289
Query: 276 LYE-------EVLEGTDGPVIFMAYPHQISDSYFLFVTERGTIYGYEVTKKALFTISQVK 328
++ +V+ + V ++ H D ++ + T + L ++
Sbjct: 290 IWSVPNASCVQVVRAHESAVTGLSL-HATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDET 348
Query: 329 LNDSVQFVNYHKDGALLAIGNQNEGVYVINLLAATKDPIKFSTGFPAKRVDFGLNGYWMF 388
S+ +H DG + G + + + +L T P + F NGY++
Sbjct: 349 SGCSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLA 408
Query: 389 VFG-DSDLNVYDLRKDPGTLAMEPLSFAENIISVELDDTAKGFFVSMGNSQVQWY 442
DS + ++DLRK + L N L G ++++G + VQ Y
Sbjct: 409 TAADDSSVKLWDLRK---LKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIY 460
>CE02043 [R] KOG0289 mRNA splicing factor
Length = 509
Score = 102 bits (255), Expect = 1e-21
Identities = 118/477 (24%), Positives = 208/477 (42%), Gaps = 74/477 (15%)
Query: 3 CAISGKPPIKAVLSPNSKCIFEQHLIEQYIEQKGTDPITDDPLQKTDLVEINATPQQIXX 62
C ISG+ V+S S IF++ LI ++I + GTDPI+ L + LV + +
Sbjct: 22 CGISGELTEDPVVSQVSGHIFDRRLIVKFIAENGTDPISHGELSEDQLVSLKS------- 74
Query: 63 XXXXXXXTIANNYSIPSLLSTLQKEWDAVMLENFELRKQLDVCKKNLSDTLYRFDXXXXX 122
+ SIPSLL LQ EWD VML +F LR+QL + ++ LS +LY+ D
Sbjct: 75 GGTGSAPRNVSGTSIPSLLKMLQDEWDTVMLNSFSLRQQLQIARQELSHSLYQHDAACRV 134
Query: 123 XXXXFVERDQLKQELAELTSAITSSNVENDGTIDKLQNSDQILSDFTSELSTKSQEYVTN 182
E ++ L+ L TS+ V++D +ID+ ++ + ++L KS+
Sbjct: 135 ISRLSKELTAAREALSTL-KPHTSAKVDDDVSIDESEDQQGLSEAILAKLEEKSKSLTAE 193
Query: 183 SKKSKK-------------KLQHFSNVSVVQSTDFSEIDLISRNQHVNLN---ERSVVVK 226
K+ K +L+ ++ + + ST I + +++L +++VV+
Sbjct: 194 RKQRGKNLPEGLAKTEELAELKQTASHTGIHSTGTPGITALDIKGNLSLTGGIDKTVVLY 253
Query: 227 DNDK---------------AFVFGPQDSILDVSQATEYLVEFKDVLYTFTQGLLAAYDLH 271
D +K A V P D+I +S + + + + ++ ++ D+H
Sbjct: 254 DYEKEQVMQTFKGHNKKINAVVLHP-DNITAISASADSHIRVWSATDSSSKAII---DVH 309
Query: 272 TKSLLYEEVLEGTDGPVIFMAYPHQISDSYFLFVTERGTIYGYEVTKKALFTISQVKLND 331
+ ++ G I A DSY+ F R G + K ++ SQ+
Sbjct: 310 QAPV--TDISLNASGDYILSA----SDDSYWAFSDIRS---GKSLCKVSVEPGSQI---- 356
Query: 332 SVQFVNYHKDGALLAIGNQNEGVYVINLLAATKDPIKFSTGFP-----AKRVDFGLNGYW 386
+V + +H DG + G + V + +L T + FP + + F NGY+
Sbjct: 357 AVHSIEFHPDGLIFGTGAADAVVKIWDLKNQT-----VAAAFPGHTAAVRSIAFSENGYY 411
Query: 387 MFVFG-DSDLNVYDLR--KDPGTLAMEPLSFAENIISVELDDTAKGFFVSMGNSQVQ 440
+ D ++ ++DLR K+ T A E + I S+ D T G F+ +G +VQ
Sbjct: 412 LATGSEDGEVKLWDLRKLKNLKTFANEE---KQPINSLSFDMT--GTFLGIGGQKVQ 463
>7302603 [R] KOG0289 mRNA splicing factor
Length = 505
Score = 80.9 bits (198), Expect = 4e-15
Identities = 104/492 (21%), Positives = 192/492 (38%), Gaps = 84/492 (17%)
Query: 1 MFCAISGKPPIKAVLSPNSKCIFEQHLIEQYIEQKGTDPITDDPLQKTDLVEINATPQQI 60
+ CA++ + P V+SP+S +FE+ +IE+Y+ + G DPI+ L+ +L+EI TP +
Sbjct: 3 LVCALTNEVPETPVVSPHSGAVFEKRVIEKYLLENGCDPISGKELKPEELIEIK-TPAVV 61
Query: 61 XXXXXXXXXTIANNYSIPSLLSTLQKEWDAVMLENFELRKQLDVCKKNLSDTLYRFDXXX 120
SIP+ L T+Q EWDA+M+ +F R+QL ++ LS LY+ D
Sbjct: 62 KPKPPSAT-------SIPATLKTMQDEWDALMIHSFTQRQQLQTTRQELSHALYQHDAAC 114
Query: 121 XXXXXXFVERDQLKQELAELTSAITSSN---------------------VENDG----TI 155
E ++ LA L +N +E G I
Sbjct: 115 RVIARLNKEVAAAREALATLKPQAGIANAPTAIPQPALASEAGGAAAHPMEQAGMSAEVI 174
Query: 156 DKLQNSDQILSDFTSELSTKSQEYVTNSKKSKKKLQ-------HFSNVSVVQSTDFSEID 208
KLQ+ +L+ + E + + + K L H ++V + + D + D
Sbjct: 175 QKLQDKATVLTQERKKRGRTVPEDLVTTDQVKNFLTVASHPGLHSASVPGILALDINSAD 234
Query: 209 LISRNQHVNLNERSVVVKDNDKAFVFGPQDSILDVSQATEYLVEFKDVLYTFTQG--LLA 266
++ NDK +D+ V+ + + V+Y + +
Sbjct: 235 -----------HSKILTGGNDKNATVFNKDTEQMVAILKGHTKKITKVIYHPNEDTVITG 283
Query: 267 AYDL-----HTKSLLYEEVLEGTDGPVIFMAYPHQISDSYFLFVTERGTIYGY-EVTKKA 320
+ D+ H + + +L +GPV ++ H D +L T + + ++
Sbjct: 284 SPDMNIRIWHVPTSQTQLLLRCHEGPVTGLSL-HPTGD--YLLSTSSDKHWAFSDIRTGR 340
Query: 321 LFTISQVKLNDSVQFVNYHKDGALLAIGNQNEGVYVINLLAATKDPIKFSTGFPAKRVDF 380
L T + +H DG + G + V + +L + P + F
Sbjct: 341 LLTKVIDTAEVGLTTAQFHPDGLIFGTGTVDSQVKIWDLKEQSNVANFPGHTGPISAISF 400
Query: 381 GLNGYWMFVFG-DSDLNVYDLRKDPGTLAMEPLSFAENIISVELDD---------TAKGF 430
NGY++ D+ + ++DLRK +N +++LDD G
Sbjct: 401 SENGYYLATAADDACVKLWDLRK------------LKNFKTIQLDDGYEVKDLCFDQSGT 448
Query: 431 FVSMGNSQVQWY 442
++++ S V+ Y
Sbjct: 449 YLAIAGSDVRVY 460
>At1g04510 [R] KOG0289 mRNA splicing factor
Length = 535
Score = 74.3 bits (181), Expect = 4e-13
Identities = 51/155 (32%), Positives = 72/155 (45%), Gaps = 28/155 (18%)
Query: 1 MFCAISGKPPIKAVLSPNSKCIFEQHLIEQYIEQKGTDPITDDPLQKTDLVEINA----T 56
M CAISG+ P + V+S S ++E+ LI+ +I G P+T +P D+V I
Sbjct: 1 MNCAISGEVPEEPVVSKKSGLLYEKRLIQTHISDYGKCPVTGEPHTLDDIVPIKTGKIVK 60
Query: 57 PQQIXXXXXXXXXTIANNYSIPSLLSTLQK------------EWDAVMLENFELRKQLDV 104
P+ + SIP LL T Q EWD++ML NF L +QL
Sbjct: 61 PKPLHTA------------SIPGLLGTFQTDHIELKWMNLIWEWDSLMLSNFALEQQLHT 108
Query: 105 CKKNLSDTLYRFDXXXXXXXXXFVERDQLKQELAE 139
++ LS LY+ D ERD+ +Q LAE
Sbjct: 109 ARQELSHALYQHDAACRVIARLKKERDESRQLLAE 143
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.134 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,275,022
Number of Sequences: 60738
Number of extensions: 1111268
Number of successful extensions: 3478
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3461
Number of HSP's gapped (non-prelim): 12
length of query: 491
length of database: 30,389,216
effective HSP length: 110
effective length of query: 381
effective length of database: 23,708,036
effective search space: 9032761716
effective search space used: 9032761716
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)