ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV1154 good A KOG2863 RNA processing and modification RNA lariat debranching enzyme
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV1154 380305 381459 385
(385 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL149c [A] KOG2863 RNA lariat debranching enzyme 325 5e-89
7295161 [A] KOG2863 RNA lariat debranching enzyme 233 3e-61
Hs7705891 [A] KOG2863 RNA lariat debranching enzyme 227 3e-59
SPAC17A5.02c [A] KOG2863 RNA lariat debranching enzyme 226 4e-59
At4g31770 [A] KOG2863 RNA lariat debranching enzyme 212 7e-55
CE09019 [A] KOG2863 RNA lariat debranching enzyme 189 8e-48
>YKL149c [A] KOG2863 RNA lariat debranching enzyme
Length = 405
Score = 325 bits (834), Expect = 5e-89
Identities = 169/404 (41%), Positives = 235/404 (57%), Gaps = 20/404 (4%)
Query: 1 MKRLQVAIQGCAHGQLQHIYDSLKTL--KTKPDILLILGDFQSLLTKYDYKTCAIPPKYA 58
M +L++A+QGC HGQL IY + + KT D+L+ILGDFQS+ D+K+ AIPPKY
Sbjct: 1 MTKLRIAVQGCCHGQLNQIYKEVSRIHAKTPIDLLIILGDFQSIRDGQDFKSIAIPPKYQ 60
Query: 59 KLGDFHKYYTGQLEAPILTIFIGGNHENMGQLTKIPHGGYIAKNIFYMGACNVITLMGVR 118
+LGDF YY ++EAP+ TIFIGGNHE+M L +PHGGY+AKNIFYMG NVI G+R
Sbjct: 61 RLGDFISYYNNEIEAPVPTIFIGGNHESMRHLMLLPHGGYVAKNIFYMGYSNVIWFKGIR 120
Query: 119 MAGISGIYNEFDHLRERPDWDQID---WNREKRSTYHVRDSEIVPLAMMRERISLVMSHD 175
+ +SGI+ E+D ++RPDW+ ++ W R+ YHVR S+I PL M++ RI +++SHD
Sbjct: 121 IGSLSGIWKEWDFNKQRPDWNDLENNNWKANIRNLYHVRISDIAPLFMIKHRIDIMLSHD 180
Query: 176 WPTSIAKHGDLKKLLKQKPYFRDEINDNSLGSPLNWYLLKRLKPIWWASAHLHVRFEATY 235
WP + HGD K LLK KP+F +I + LGSP+ W LL+ L+P WW SAHLHVRF A+
Sbjct: 181 WPNGVVYHGDTKHLLKLKPFFEQDIKEGKLGSPVTWQLLRDLRPQWWLSAHLHVRFMASI 240
Query: 236 KHD---HKVNNXXXXXXXXXXX------XXXXXXKPGISTQATSTKINTT---TKFIALD 283
KH+ H+ N + GI + ++ T+F+ALD
Sbjct: 241 KHNKRSHEPPNKSTSKTKKNNNEIDLDLSSDEDERSGIMNCQEENEYDSKYGETRFLALD 300
Query: 284 KCKGGNSFRHLTEITIDYDENHPTCQDRALYWDPEYLANLKYCLSIKDSLKGKDLQQIPV 343
KC + + EI D ++ ++WDPE++ NL C K+ L K +
Sbjct: 301 KCLPRRRWLEILEIEPDTSHASWKDENHRMFWDPEFINNLVICQKNKNLLSNKPFNSVNW 360
Query: 344 DEL-QSNVEVGHVEDWSKYRIP--VPQSYRSVIKQTEYVTEKFL 384
EL QSN E G DW Y IP + ++QT+ KF+
Sbjct: 361 IELSQSNREEGRDIDWENYAIPAYTLDIQKDEVRQTKAFISKFM 404
>7295161 [A] KOG2863 RNA lariat debranching enzyme
Length = 534
Score = 233 bits (595), Expect = 3e-61
Identities = 127/340 (37%), Positives = 195/340 (57%), Gaps = 17/340 (5%)
Query: 4 LQVAIQGCAHGQLQHIYDSLKTLK----TKPDILLILGDFQSLLTKYDYKTCAIPPKYAK 59
+++A++GCAHG+L+ IYD+++ ++ TK D+LL GDFQS D +T A+P KY
Sbjct: 1 MKIAVEGCAHGELERIYDTIEGIEKVGGTKIDLLLCCGDFQSTRNLEDLQTMAVPKKYLD 60
Query: 60 LGDFHKYYTGQLEAPILTIFIGGNHENMGQLTKIPHGGYIAKNIFYMGACNVITLMGVRM 119
+ F+KYY+G+L AP+LTIFIGGNHE L ++P+GG++A NI+Y+G V+ + GVR+
Sbjct: 61 MCSFYKYYSGELVAPVLTIFIGGNHEASNYLQELPYGGWVAPNIYYLGYAGVVNVNGVRI 120
Query: 120 AGISGIYNEFDHLRERPDWDQIDWNREKRSTYHVRDSEIVPLAMMRERISLVMSHDWPTS 179
AGISGI+ D LR ++ + RS YHVR E+ L + R+ + +SHDWPT
Sbjct: 121 AGISGIFKGHDFLRGHHEFPPYT-DSTCRSVYHVRQLEVFRLKQISGRVDIFLSHDWPTG 179
Query: 180 IAKHGDLKKLLKQKPYFRDEINDNSLGSPLNWYLLKRLKPIWWASAHLHVRFEATYKHDH 239
I ++G+ +LL++KP+F ++ LGS LLK ++P +W +AHLH +F A H+H
Sbjct: 180 IYEYGNKAQLLRKKPFFAADMESGKLGSQPLEELLKAVQPAYWFAAHLHCKFAALVPHNH 239
Query: 240 KVNNXXXXXXXXXXXXXXXXXKPGISTQAT----STKINTTTKFIALDKCKGGNSFRHLT 295
+ +A +K TKF+ALDKC +F +
Sbjct: 240 SQKLGDAESSSSSSSSEDEDEEREKVKKAAPVPPPSKSVPVTKFLALDKCLPRRAFLQVV 299
Query: 296 EITIDYDENHPTCQDRALYWDPEYLANL---KYCLSIKDS 332
E+ D E P L +D E+LA L + +S+K++
Sbjct: 300 EVPSDPIEGTP-----RLEYDAEWLAILHSTNHLISVKEN 334
>Hs7705891 [A] KOG2863 RNA lariat debranching enzyme
Length = 544
Score = 227 bits (578), Expect = 3e-59
Identities = 126/328 (38%), Positives = 190/328 (57%), Gaps = 45/328 (13%)
Query: 4 LQVAIQGCAHGQLQHIYDSLKTLKTKP----DILLILGDFQSLLTKYDYKTCAIPPKYAK 59
++VA+ GC HG+L IY++L + + D+LL GDFQ++ + D + A+PPKY
Sbjct: 1 MRVAVAGCCHGELDKIYETLALAERRGPGPVDLLLCCGDFQAVRNEADLRCMAVPPKYRH 60
Query: 60 LGDFHKYYTGQLEAPILTIFIGGNHENMGQLTKIPHGGYIAKNIFYMGACNVITLMGVRM 119
+ F++YY+G+ +AP+LT+FIGGNHE L ++P+GG++A NI+Y+G V+ GVR+
Sbjct: 61 MQTFYRYYSGEKKAPVLTLFIGGNHEASNHLQELPYGGWVAPNIYYLGLAGVVKYRGVRI 120
Query: 120 AGISGIYNEFDHLR---ERPDWDQIDWNREKRSTYHVRDSEIVPLAMMRERISLVMSHDW 176
GISGI+ D+ + E P ++ + RS YHVR+ E+ L +++ I + +SHDW
Sbjct: 121 GGISGIFKSHDYRKGHFECPPYN----SSTIRSIYHVRNIEVYKLKQLKQPIDIFLSHDW 176
Query: 177 PTSIAKHGDLKKLLKQKPYFRDEINDNSLGSPLNWYLLKRLKPIWWASAHLHVRFEATYK 236
P SI +G+ K+LLK K +FR E+ +N+LGSP LL+ LKP +W SAHLHV+F A +
Sbjct: 177 PRSIYHYGNKKQLLKTKSFFRQEVENNTLGSPAASELLEHLKPTYWFSAHLHVKFAALMQ 236
Query: 237 HDHKVNNXXXXXXXXXXXXXXXXXKPGISTQATSTKINTTTKFIALDKCKGGNSFRHLTE 296
H K G + +A TKF+ALDKC F + E
Sbjct: 237 HQAK--------------------DKGQTARA--------TKFLALDKCLPHRDFLQILE 268
Query: 297 ITIDYDENHPTCQDRALYWDPEYLANLK 324
I E+ P+ D L +D E+L L+
Sbjct: 269 I-----EHDPSAPD-YLEYDIEWLTILR 290
>SPAC17A5.02c [A] KOG2863 RNA lariat debranching enzyme
Length = 466
Score = 226 bits (576), Expect = 4e-59
Identities = 107/237 (45%), Positives = 162/237 (68%), Gaps = 5/237 (2%)
Query: 3 RLQVAIQGCAHGQLQHIYDSLKTLKTKPDILLILGDFQSLLTKYDYKTCAIPPKYAKLGD 62
+++V +QGC HG L ++Y + K K D+L+I GDFQ+L DY ++PPK+ +LGD
Sbjct: 15 KMRVGVQGCCHGILDNLY--ILAEKRKVDLLIIGGDFQALRNVSDYHGISMPPKFKRLGD 72
Query: 63 FHKYYTGQLEAPILTIFIGGNHENMGQLTKIPHGGYIAKNIFYMGACNVITLMGVRMAGI 122
F YY G+ +APILTIF+GGNHE L ++P+GG++A NI+YMG +VI + G+R+AGI
Sbjct: 73 FFNYYNGRNKAPILTIFVGGNHEASNYLDELPYGGWVAPNIYYMGRSSVINVGGLRIAGI 132
Query: 123 SGIYNEFDHLRERPDWDQIDWN-REKRSTYHVRDSEIVPLAMMRERISLVMSHDWPTSIA 181
SGIY+ D+ + R ++ + +N + +S YH R+ +++ L +++ I + +SHDWP I
Sbjct: 133 SGIYSAMDYKKGR--YEGLPYNYKMLKSIYHTREFDVLSLKSLQKPIDIFLSHDWPRGIE 190
Query: 182 KHGDLKKLLKQKPYFRDEINDNSLGSPLNWYLLKRLKPIWWASAHLHVRFEATYKHD 238
+HGD+ KLL+ KP+FR+E+ N LGSP LL LKP +W +AHLH +F A H+
Sbjct: 191 QHGDVAKLLRHKPFFRNEVERNDLGSPALEELLVELKPRYWMAAHLHTKFTAVVHHN 247
>At4g31770 [A] KOG2863 RNA lariat debranching enzyme
Length = 401
Score = 212 bits (540), Expect = 7e-55
Identities = 116/302 (38%), Positives = 169/302 (55%), Gaps = 46/302 (15%)
Query: 4 LQVAIQGCAHGQLQHIYDSLKTLK----TKPDILLILGDFQSLLTKYDYKTCAIPPKYAK 59
+++AI+GC HG L ++Y +++ + TK D+LL GDFQ++ + D + +P KY +
Sbjct: 1 MKIAIEGCMHGDLDNVYKTIQHYEQIHNTKVDLLLCCGDFQAVRNEKDMDSLNVPRKYRE 60
Query: 60 LGDFHKYYTGQLEAPILTIFIGGNHENMGQLTKIPHGGYIAKNIFYMGACNVITLMGVRM 119
+ F KYY+GQ API TIFIGGNHE L ++ +GG+ A NI+++G V+ VR+
Sbjct: 61 MKSFWKYYSGQEVAPIPTIFIGGNHEASNYLWELYYGGWAATNIYFLGFAGVVKFGNVRI 120
Query: 120 AGISGIYNEFDHLR----ERPDWDQIDWNREKRSTYHVRDSEIVPLAMMRERISLVMSHD 175
G+SGIYNE H R ERP +++ RS YHVRD ++ L + E + + +SHD
Sbjct: 121 GGLSGIYNE-RHYRSGHFERPPYNE----STIRSVYHVRDYDVQKLMQLEEPLDIFLSHD 175
Query: 176 WPTSIAKHGDLKKLLKQKPYFRDEINDNSLGSPLNWYLLKRLKPIWWASAHLHVRFEATY 235
WP I +GD + L++QKPYFR EI + +LGS LL++LKP +W SAHLH +F A
Sbjct: 176 WPVGITDYGDSESLMRQKPYFRQEIEEKTLGSKPAALLLEKLKPQYWFSAHLHCKFAAAV 235
Query: 236 KHDHKVNNXXXXXXXXXXXXXXXXXKPGISTQATSTKINTTTKFIALDKCKGGNSFRHLT 295
+H + + TKF+ALDKC G F +
Sbjct: 236 QHGND---------------------------------GSVTKFLALDKCLPGKKFLQII 262
Query: 296 EI 297
EI
Sbjct: 263 EI 264
>CE09019 [A] KOG2863 RNA lariat debranching enzyme
Length = 504
Score = 189 bits (479), Expect = 8e-48
Identities = 115/368 (31%), Positives = 192/368 (51%), Gaps = 64/368 (17%)
Query: 2 KRLQVAIQGCAHGQLQHIYDSLKTLKTKP----DILLILGDFQSLLTKYDYKTCAIPPKY 57
K++++A+ GC+HG++ IY+++ ++ K D+L+ GD+Q++ D +IPPKY
Sbjct: 39 KKIRIAVVGCSHGEMDAIYETMTLIEQKNGYKFDLLICCGDYQAVRNYGDLPHMSIPPKY 98
Query: 58 AKLGDFHKYYTGQLEAPILTIFIGGNHENMGQLTKIPHGGYIAKNIFYMGACNVITLMGV 117
L F+KYY+G+ +AP+LT+FIGGNHE G L ++P+GG++A NI+YMG N I +
Sbjct: 99 RSLQTFYKYYSGEQKAPVLTLFIGGNHEASGYLCELPNGGWVAPNIYYMGFANCIRFANL 158
Query: 118 RMAGISGIYN----EFDHLRERPDWDQIDWNREKRSTYHVRDSEIVPLAMMR-------- 165
R+AG+SGI++ +F H ERP + + R+ +S YHVR+ ++ L ++
Sbjct: 159 RIAGLSGIFSQGDFQFSHY-ERPSFSE----RDVKSAYHVRNVDMFRLRQLKSDNENKIS 213
Query: 166 ERISLVMSHDWPTSIAKHGDLKKLLKQKPYFRDEINDNSLGSPLNWYLLKRLKPIWWASA 225
I +++SHDWP I GD + L ++K F + N LG+P L+ +P ++ +A
Sbjct: 214 NPIDIMLSHDWPGGIPDFGDKEWLFRKKDLFEADHNSGKLGNPSGMKLIYDCRPRYYLAA 273
Query: 226 HLHVRFEATYKHDHKVNNXXXXXXXXXXXXXXXXXKPGISTQATSTKINTTTKFIALDKC 285
HLH+ F A H + + + T+F++LDK
Sbjct: 274 HLHIAFAALVPH-----------------------------KGSGSGRPQPTRFLSLDKP 304
Query: 286 KGGNSF----RHLTEITIDYDENHPTCQDRALYWDPEYLANLKYCLSIKDSLKGKDLQQI 341
G F RH E+ + D L +DP++LA L+ D L +Q+
Sbjct: 305 IPGRKFMQASRHALELNVADDAK------LELSYDPQWLAILRN----TDLLTTGTKEQL 354
Query: 342 PVDELQSN 349
+ ++ SN
Sbjct: 355 ILPDMASN 362
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.137 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,573,798
Number of Sequences: 60738
Number of extensions: 1012472
Number of successful extensions: 2068
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2043
Number of HSP's gapped (non-prelim): 9
length of query: 385
length of database: 30,389,216
effective HSP length: 108
effective length of query: 277
effective length of database: 23,829,512
effective search space: 6600774824
effective search space used: 6600774824
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)