ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV1181 good O KOG3946 Posttranslational modification, protein turnover, chaperones Glutaminyl cyclase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV1181 390309 389305 -335
(335 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YFR018c [O] KOG3946 Glutaminyl cyclase 236 3e-62
7296321 [O] KOG3946 Glutaminyl cyclase 163 4e-40
Hs6912618 [O] KOG3946 Glutaminyl cyclase 161 1e-39
Hs8923092 [O] KOG3946 Glutaminyl cyclase 156 4e-38
7295416 [O] KOG3946 Glutaminyl cyclase 146 5e-35
CE17979 [O] KOG3946 Glutaminyl cyclase 139 6e-33
7294735 [O] KOG3946 Glutaminyl cyclase 103 4e-22
>YFR018c [O] KOG3946 Glutaminyl cyclase
Length = 363
Score = 236 bits (603), Expect = 3e-62
Identities = 132/329 (40%), Positives = 197/329 (59%), Gaps = 35/329 (10%)
Query: 19 EFPFKSYYDSTLADLMSINETYSGP---LLSPFNKTRVPGSKGSKEVQEFVLDEFKS-MN 74
E ++ Y+ + L + ++ + ++ LL PFN+TRVPGS+GS+E+Q F+++ F + +
Sbjct: 30 ELSYEQYHAAHLNEAINPDSGWNKSTKNLLLPFNRTRVPGSEGSREIQRFIIEHFNNTLA 89
Query: 75 QNWAVELQNFEENGYEFTNMIFSLGSNDS-YLALAAHYDSKLEPDGFIGGIDSAAPCAML 133
WAVE Q FEENGY F N++ +L +N S YL LAAHYD+K+ P G +G IDSAA CA L
Sbjct: 90 GEWAVETQAFEENGYRFNNLVMTLQNNASEYLVLAAHYDTKIAPTGMVGAIDSAASCAAL 149
Query: 134 LYLSHFIDDVI----TEDLSVIDP-TLLGQFT-GVKIIFFDGEEAFKEWTSTDSLYGSRH 187
LY + F+ + T++ + ++ T++ T GVKI+FFDGEEA +EW DS+YG+R
Sbjct: 150 LYTAQFLTHIACHERTKEYNDLESNTVVSNSTLGVKIVFFDGEEAIEEWGPEDSIYGARR 209
Query: 188 AAAKWEKDGTLDKMDLFVLLDLLG-GKESITVPNYTKAARAFYKNLLQIERTYNKFYGAS 246
AA+W DGT+ ++ L LLDLLG G+E VP+Y Y+ L +IE + +
Sbjct: 210 LAAQWLADGTMTRIRLLFLLDLLGSGEEEPLVPSYYAETHQEYQLLNRIED--DLLFRRG 267
Query: 247 DKI-FQSSVSNNIR--------------------ISDDQEPFFTRGVKTLHLIPFPFPSS 285
D+I +S+++ + I DD PF GV LH IP PFPS+
Sbjct: 268 DEINGESALAAEVARQRKHLDPTDYRFLGLGHSVIGDDHTPFLAAGVPVLHAIPLPFPST 327
Query: 286 WHTLADDYAHLDEKHIHKWCIIMCEFIIK 314
WHT+ DD+ HLD W +++CEF+++
Sbjct: 328 WHTVDDDFRHLDAAETRHWALLVCEFVVQ 356
>7296321 [O] KOG3946 Glutaminyl cyclase
Length = 352
Score = 163 bits (412), Expect = 4e-40
Identities = 106/307 (34%), Positives = 159/307 (51%), Gaps = 35/307 (11%)
Query: 28 STLADLMSINETYSGPLLSPFNKTRVPGSKGSKEVQEFVLDEFKSMNQNWAVELQNFEEN 87
S L+D + + E L+ RV G+ V+E+++ + ++ W VE+ +F ++
Sbjct: 54 SNLSDKLHLREAIDKILIP-----RVVGTTNHSIVREYIVQSLRDLD--WDVEVNSFHDH 106
Query: 88 G-----YEFTNMIFSLGSN-DSYLALAAHYDSKLEPD-GFIGGIDSAAPCAMLLYLSHFI 140
F N+I +L N + YL L+ HYDSK P F+G DSA PCAMLL L+
Sbjct: 107 APIKGKLHFHNIIATLNPNAERYLVLSCHYDSKYMPGVEFLGATDSAVPCAMLLNLAQ-- 164
Query: 141 DDVITEDLSVIDPTLLGQFTGVKIIFFDGEEAFKEWTSTDSLYGSRHAAAKWEKDGTLDK 200
V+ E L + + L + ++FFDGEEAF+EW DS+YG+RH A KW +G LD+
Sbjct: 165 --VLQEQLKPLKKSKLS----LMLLFFDGEEAFEEWGPKDSIYGARHLAKKWHHEGKLDR 218
Query: 201 MDLFVLLDLLGGKESITVPNYTKAARAFYKNLLQIERTYNKF-----YGAS-------DK 248
+D+ VLLDLLG + ++ + ++Y + +E K Y +S +
Sbjct: 219 IDMLVLLDLLGAPDP-AFYSFFENTESWYMRIQSVETRLAKLQLLERYASSGVAQRDPTR 277
Query: 249 IFQSSVSNNIRISDDQEPFFTRGVKTLHLIPFPFPSSWHTLADDYAHLDEKHIHKWCIIM 308
FQS + I DD PF R V LHLIP PFPS WHT D+ + +D +I+
Sbjct: 278 YFQSQAMRSSFIEDDHIPFLRRNVPILHLIPVPFPSVWHTPDDNASVIDYATTDNLALII 337
Query: 309 CEFIIKY 315
F ++Y
Sbjct: 338 RLFALEY 344
>Hs6912618 [O] KOG3946 Glutaminyl cyclase
Length = 361
Score = 161 bits (407), Expect = 1e-39
Identities = 114/311 (36%), Positives = 159/311 (50%), Gaps = 33/311 (10%)
Query: 30 LADLMSINETYSGPLLSPFNKTRVPGSKGSKEVQEFVLDEFKSMNQNWAVELQNFEEN-- 87
+A+ SI+E + L P R PGS GS ++ ++ + + +W +E+ F
Sbjct: 56 IAEGTSISEMWQNDL-QPLLIERYPGSPGSYAARQHIMQRIQRLQADWVLEIDTFLSQTP 114
Query: 88 -GYE-FTNMIFSLGSNDS-YLALAAHYDSKLEPDG----FIGGIDSAAPCAMLLYLSHFI 140
GY F+N+I +L +L LA HYDSK F+G DSA PCAM+L L+ +
Sbjct: 115 YGYRSFSNIISTLNPTAKRHLVLACHYDSKYFSHWNNRVFVGATDSAVPCAMMLELARAL 174
Query: 141 DDVITEDLSVID--PTLLGQFTGVKIIFFDGEEAFKEWTSTDSLYGSRHAAAKWEKD--- 195
D + +V D P L +++IFFDGEEAF W+ DSLYGSRH AAK
Sbjct: 175 DKKLLSLKTVSDSKPDL-----SLQLIFFDGEEAFLHWSPQDSLYGSRHLAAKMASTPHP 229
Query: 196 ----GT--LDKMDLFVLLDLLGGKESITVPNYTKAARAFYKNLLQIERTYN-----KFYG 244
GT L MDL VLLDL+G T PN+ + +++ L IE + K +
Sbjct: 230 PGARGTSQLHGMDLLVLLDLIGAPNP-TFPNFFPNSARWFERLQAIEHELHELGLLKDHS 288
Query: 245 ASDKIFQSSVSNNIRISDDQEPFFTRGVKTLHLIPFPFPSSWHTLADDYAHLDEKHIHKW 304
+ FQ+ + I DD PF RGV LHLIP PFP WHT+ D+ +LDE I
Sbjct: 289 LEGRYFQNYSYGGV-IQDDHIPFLRRGVPVLHLIPSPFPEVWHTMDDNEENLDESTIDNL 347
Query: 305 CIIMCEFIIKY 315
I+ F+++Y
Sbjct: 348 NKILQVFVLEY 358
>Hs8923092 [O] KOG3946 Glutaminyl cyclase
Length = 382
Score = 156 bits (395), Expect = 4e-38
Identities = 105/296 (35%), Positives = 149/296 (49%), Gaps = 31/296 (10%)
Query: 39 TYSGPLLSPFNKTRVPGSKGSKEVQEFVLDEFKSMNQNWAVELQNFEENG----YEFTNM 94
TY PLL R PGS G+ +V++F+ +S+ W VEL F + +F N+
Sbjct: 96 TYLRPLLV----VRTPGSPGNLQVRKFLEATLRSLTAGWHVELDPFTASTPLGPVDFGNV 151
Query: 95 IFSLGSNDS-YLALAAHYDSKLEPDG---FIGGIDSAAPCAMLLYLSHFIDDVITEDLSV 150
+ +L + +L LA HYDSKL P G F+G DSA PCA+LL L+ +D ++
Sbjct: 152 VATLDPRAARHLTLACHYDSKLFPPGSTPFVGATDSAVPCALLLELAQALDLELSRAKKQ 211
Query: 151 IDPTLLGQFTGVKIIFFDGEEAFKEWTSTDSLYGSRHAAAKWEK------DGTLDKMDLF 204
P L +++F DGEEA KEW DSLYGSRH A E + ++LF
Sbjct: 212 AAPVTL------QLLFLDGEEALKEWGPKDSLYGSRHLAQLMESIPHSPGPTRIQAIELF 265
Query: 205 VLLDLLGGKESITVPNYTKAARAFYKNLLQIERTYNKF-----YGASDKIFQSSVSNNIR 259
+LLDLLG ++ + R F++ L IE+ ++ + FQ +
Sbjct: 266 MLLDLLGAPNPTFYSHFPRTVRWFHR-LRSIEKRLHRLNLLQSHPQEVMYFQPGEPSG-S 323
Query: 260 ISDDQEPFFTRGVKTLHLIPFPFPSSWHTLADDYAHLDEKHIHKWCIIMCEFIIKY 315
+ DD PF RGV LHLI PFP+ WHT AD +L +H C I+ F+ +Y
Sbjct: 324 VEDDHIPFLRRGVPVLHLISTPFPAVWHTPADTEVNLHPPTVHNLCRILAVFLAEY 379
>7295416 [O] KOG3946 Glutaminyl cyclase
Length = 626
Score = 146 bits (368), Expect = 5e-35
Identities = 98/305 (32%), Positives = 160/305 (52%), Gaps = 39/305 (12%)
Query: 48 FNKT-------RVPGSKGSKEVQEFVLDEFKSMNQNWAVELQNFEEN-----GYEFTNMI 95
FN+T RV GS+G ++V+E+++ + + E+ F++ F N++
Sbjct: 327 FNRTLDSILVPRVVGSRGHQQVREYLVQSLNGLG--FQTEVDEFKQRVPVFGELTFANVV 384
Query: 96 FSLGSN-DSYLALAAHYDSKLEPD--GFIGGIDSAAPCAMLLYLSHFIDDVITEDLSVID 152
++ ++LALA HYDSK P+ GF+G DSA PCA+LL + + + ++
Sbjct: 385 GTINPQAQNFLALACHYDSKYFPNDPGFVGATDSAVPCAILLNTAKTLGAYLQKEFRNRS 444
Query: 153 PTLLGQFTGVKIIFFDGEEAFKEWTSTDSLYGSRHAAAKW--EKDGT--------LDKMD 202
G+ +IFFDGEEAFKEWT DS+YGS+H AAK ++ G+ +D+++
Sbjct: 445 DV------GLMLIFFDGEEAFKEWTDADSVYGSKHLAAKLASKRSGSQAQLAPRNIDRIE 498
Query: 203 LFVLLDLLGGKESITVPNYTKAARAFYKNLLQIE---RTYNKFYGASDKIFQSSVSNNIR 259
+ VLLDL+G + ++ + + +L+QIE RT + G ++ +F S VS +
Sbjct: 499 VLVLLDLIGARNP-KFSSFYENTDGLHSSLVQIEKSLRTAGQLEG-NNNMFLSRVSGGL- 555
Query: 260 ISDDQEPFFTRGVKTLHLIPFPFPSSWHTLADDYAHLDEKHIHKWCIIMCEFIIKYWNPD 319
+ DD PF V LHL+ PFP WHT D+ A+L I + + F+ +Y
Sbjct: 556 VDDDHRPFLDENVPVLHLVATPFPDVWHTPRDNAANLHWPSIRNFNRVFRNFVYQYLKRH 615
Query: 320 KVPLH 324
P++
Sbjct: 616 TSPVN 620
Score = 51.6 bits (122), Expect = 2e-06
Identities = 41/123 (33%), Positives = 59/123 (47%), Gaps = 12/123 (9%)
Query: 37 NETYSGPLLSPFNKTRVPGSKGSKEVQEFVLDEFKSMNQNWAVELQNFEENGYEFTNMI- 95
+E + L+ K R GSKG EVQ+F+ E K ++ + L+N + G TN++
Sbjct: 17 DEEHFNATLAKLLKPRSVGSKGHAEVQDFIETELKRLD---FITLKNDFQQGVNITNLVG 73
Query: 96 FSLGSNDSYLALAAHYDSK----LEPDGFIGGIDSAAPCAMLL----YLSHFIDDVITED 147
F YL L HYDSK + D F+ + A CA+LL L F+ D +E
Sbjct: 74 FWNMRAKFYLMLTCHYDSKKPENVTTDEFLAAAEGAVSCAILLNVAKTLRQFLIDRWSEK 133
Query: 148 LSV 150
SV
Sbjct: 134 KSV 136
>CE17979 [O] KOG3946 Glutaminyl cyclase
Length = 342
Score = 139 bits (350), Expect = 6e-33
Identities = 101/302 (33%), Positives = 144/302 (47%), Gaps = 34/302 (11%)
Query: 37 NETYSGPLLSPFNKTRVPGSKGSKEVQEFVLDEFKSMNQNWAVELQNFEENG----YEFT 92
N T +L+P R+ +K ++V ++ L F N +A E F + F
Sbjct: 46 NTTRFKEILAPIMVPRIVDTKQHRQVGDY-LQSFLH-NLGFATEWDAFTDTTPLGTRNFR 103
Query: 93 NMIFSLG-SNDSYLALAAHYDSKLEPDG-FIGGIDSAAPCAMLLYLSHFIDDVITEDLSV 150
N+I + S L LA HYDSK+ P I DSA PCAM+L ++ + + + ++
Sbjct: 104 NLIATFDESAPRRLVLACHYDSKIIPGQVMIAATDSAVPCAMMLDIAQTLAPYMYKRVA- 162
Query: 151 IDPTLLGQFTGVKIIFFDGEEAFKEWTSTDSLYGSRHAAAKWEK--------------DG 196
Q G+++IFFDGEEAF++WT+TDSLYGSRH A KWE+
Sbjct: 163 -------QQIGLQLIFFDGEEAFRDWTATDSLYGSRHLAQKWEQKWYPSSSSLNNFELSK 215
Query: 197 TLDKMDLFVLLDLLGGKESITVPNYTKAARAFYKNLLQIE---RTYNKFYGASDKIFQSS 253
LD++D+ +LLDLLG A + L +E RT +F
Sbjct: 216 ELDRIDVLMLLDLLGAANPSIGNTIGMGANDLFSQLADVESNLRTSGCLSSLRRNVFNKQ 275
Query: 254 VSNNIRISDDQEPFFTRGVKTLHLIPFPFPSSWHTLADDYAHLDEKHIHKWCIIMCEFII 313
+S N ++ DD PF RGV LHLI PFPS WH +D+ L I ++ F+
Sbjct: 276 LSYN-QVEDDHIPFLKRGVPILHLITVPFPSVWHRSSDNANALHYPTIDHMTAVIRVFVA 334
Query: 314 KY 315
KY
Sbjct: 335 KY 336
>7294735 [O] KOG3946 Glutaminyl cyclase
Length = 205
Score = 103 bits (257), Expect = 4e-22
Identities = 56/144 (38%), Positives = 77/144 (52%), Gaps = 21/144 (14%)
Query: 164 IIFFDGEEAFKEWTSTDSLYGSRHAAAKWEKDGTLDKMDLFVLLDLLGGKESITVPNYTK 223
++FFDGEEAF EW+ DS YGSRH A WEK G LDK+DLFVL DL+G K+ +
Sbjct: 36 LVFFDGEEAFGEWSQEDSPYGSRHLAELWEKHGFLDKIDLFVLPDLIGAKDVV------- 88
Query: 224 AARAFYKNLLQIERTYNKFYGASDKIFQSSVSNN----------IRISDDQEPFFTRGVK 273
F KN+L +++ K+FQ+ + + I + DD PF R V
Sbjct: 89 ----FKKNILDTSGWFHRLVQLELKLFQAGIVRSERPLFKFEPGIDVDDDHLPFTRRNVP 144
Query: 274 TLHLIPFPFPSSWHTLADDYAHLD 297
+HLI +P+ WH D ++D
Sbjct: 145 VIHLISNKYPAVWHQAEDVEMNVD 168
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.138 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,389,824
Number of Sequences: 60738
Number of extensions: 968445
Number of successful extensions: 2046
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2009
Number of HSP's gapped (non-prelim): 10
length of query: 335
length of database: 30,389,216
effective HSP length: 107
effective length of query: 228
effective length of database: 23,890,250
effective search space: 5446977000
effective search space used: 5446977000
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)