ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV1273 good E KOG1549 Amino acid transport and metabolism Cysteine desulfurase NFS1
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV1273 424042 422552 -497
(497 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YCL017c [E] KOG1549 Cysteine desulfurase NFS1 748 0.0
SPBC21D10.11c [E] KOG1549 Cysteine desulfurase NFS1 575 e-164
Hs20560255 [E] KOG1549 Cysteine desulfurase NFS1 554 e-157
7297897 [E] KOG1549 Cysteine desulfurase NFS1 547 e-155
CE17320 [E] KOG1549 Cysteine desulfurase NFS1 531 e-151
Hs10864079 [E] KOG1549 Cysteine desulfurase NFS1 520 e-147
At5g65720 [E] KOG1549 Cysteine desulfurase NFS1 517 e-146
ECU11g1770 [E] KOG1549 Cysteine desulfurase NFS1 508 e-144
Hs19923803 [E] KOG1549 Cysteine desulfurase NFS1 201 2e-51
CE02643 [E] KOG1549 Cysteine desulfurase NFS1 169 9e-42
At1g08490 [E] KOG1549 Cysteine desulfurase NFS1 108 1e-23
SPAC11D3.10 [E] KOG1549 Cysteine desulfurase NFS1 64 5e-10
Hs8923661 [H] KOG2142 Molybdenum cofactor sulfurase 57 8e-08
7297896 [E] KOG1549 Cysteine desulfurase NFS1 52 3e-06
SPCC777.03c [E] KOG1549 Cysteine desulfurase NFS1 51 3e-06
At1g27980 [E] KOG1383 Glutamate decarboxylase/sphingosine phosph... 50 8e-06
>YCL017c [E] KOG1549 Cysteine desulfurase NFS1
Length = 497
Score = 748 bits (1932), Expect = 0.0
Identities = 369/460 (80%), Positives = 416/460 (90%), Gaps = 4/460 (0%)
Query: 42 SAKVALAEDLTLETHTDNQAAAIEQANERAVSN---PD-VLDSGIHSLQHAYRESGEYGT 97
+A V L ++ +LETHTD QAAA QA+ RA ++ PD V+ SG ++ HAY+E+ +GT
Sbjct: 38 AAGVKLDDNFSLETHTDIQAAAKAQASARASASGTTPDAVVASGSTAMSHAYQENTGFGT 97
Query: 98 RPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNKEIEQARKYIADVIKA 157
RPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETN +E AR ++A +I A
Sbjct: 98 RPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNTAVENARAHVAKMINA 157
Query: 158 DPKEIIFTSGATESNNMALKGVSRFYKKTRNHIITTRTEHKCVLEAARAMKNEGYEITFL 217
DPKEIIFTSGATESNNM LKGV RFYKKT+ HIITTRTEHKCVLEAARAM EG+E+TFL
Sbjct: 158 DPKEIIFTSGATESNNMVLKGVPRFYKKTKKHIITTRTEHKCVLEAARAMMKEGFEVTFL 217
Query: 218 NVDEQGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKEIGELCRKRKVFFHTDAAQA 277
NVD+QGLI+L+ELE AIRP+TCLVSVMAVNNEIGV+QP+KEIG +CRK K++FHTDAAQA
Sbjct: 218 NVDDQGLIDLKELEDAIRPDTCLVSVMAVNNEIGVIQPIKEIGAICRKNKIYFHTDAAQA 277
Query: 278 YGKIPIDVNEMKIDLMSISSHKIYGPKGIGAIYVRRKPRVRLDPLISGGGQERGLRSGTL 337
YGKI IDVNEM IDL+SISSHKIYGPKGIGAIYVRR+PRVRL+PL+SGGGQERGLRSGTL
Sbjct: 278 YGKIHIDVNEMNIDLLSISSHKIYGPKGIGAIYVRRRPRVRLEPLLSGGGQERGLRSGTL 337
Query: 338 APPLVAGFGEAARLMMKEYENDSNHIKRLSDKLMNALLSIDHTILNGSADHRYPGCVNIS 397
APPLVAGFGEAARLM KE++ND HIKRLSDKL+ LLS +HT LNGS DHRYPGCVN+S
Sbjct: 338 APPLVAGFGEAARLMKKEFDNDQAHIKRLSDKLVKGLLSAEHTTLNGSPDHRYPGCVNVS 397
Query: 398 FAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDALAHSSIRFGIGRFTTDE 457
FAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDALAHSSIRFGIGRF+T+E
Sbjct: 398 FAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDALAHSSIRFGIGRFSTEE 457
Query: 458 EIDYVIKAITERVDFLRELSPLWEMVKEGIDLNSIEWSGH 497
E+DYV+KA+++RV FLRELSPLWEMV+EGIDLNSI+WSGH
Sbjct: 458 EVDYVVKAVSDRVKFLRELSPLWEMVQEGIDLNSIKWSGH 497
>SPBC21D10.11c [E] KOG1549 Cysteine desulfurase NFS1
Length = 498
Score = 575 bits (1483), Expect = e-164
Identities = 277/402 (68%), Positives = 334/402 (82%)
Query: 96 GTRPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNKEIEQARKYIADVI 155
G PIY+D QAT+P D RVLD+ML F+TG+YGNPHS TH+YGWE K +E AR+ IA VI
Sbjct: 97 GLHPIYMDFQATSPLDYRVLDSMLPFFTGIYGNPHSRTHAYGWEAEKAVENARQEIASVI 156
Query: 156 KADPKEIIFTSGATESNNMALKGVSRFYKKTRNHIITTRTEHKCVLEAARAMKNEGYEIT 215
ADP+EIIFTSGATESNN LKGV+RFYK + H+++ +TEHKCVL++ RA++ EG+E+T
Sbjct: 157 NADPREIIFTSGATESNNAILKGVARFYKSRKKHLVSVQTEHKCVLDSLRALQEEGFEVT 216
Query: 216 FLNVDEQGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKEIGELCRKRKVFFHTDAA 275
FL V GLINL+EL AIRP+T VSVMAVNNEIGV QPL+EIG++CR++KVFFH+DAA
Sbjct: 217 FLPVQTNGLINLDELRDAIRPDTVCVSVMAVNNEIGVCQPLEEIGKICRQKKVFFHSDAA 276
Query: 276 QAYGKIPIDVNEMKIDLMSISSHKIYGPKGIGAIYVRRKPRVRLDPLISGGGQERGLRSG 335
Q YGKI IDVN M IDLMSIS+HKIYGPKGIGA YVRR+PRVRL+PLISGGGQERGLRSG
Sbjct: 277 QGYGKIDIDVNRMNIDLMSISAHKIYGPKGIGAAYVRRRPRVRLEPLISGGGQERGLRSG 336
Query: 336 TLAPPLVAGFGEAARLMMKEYENDSNHIKRLSDKLMNALLSIDHTILNGSADHRYPGCVN 395
TLAP V GFG AAR+ +E + D HI +LS +L++ LL+I +T LNG RYPGCVN
Sbjct: 337 TLAPSQVVGFGTAARICKEEMKYDYAHISKLSQRLIDGLLAIPYTSLNGDPKSRYPGCVN 396
Query: 396 ISFAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDALAHSSIRFGIGRFTT 455
ISF YVEGESLLM L++IALSSGSACTSASLEPSYVL A+G+ D AHSSIRFGIGRFTT
Sbjct: 397 ISFNYVEGESLLMGLKNIALSSGSACTSASLEPSYVLRAIGQSDENAHSSIRFGIGRFTT 456
Query: 456 DEEIDYVIKAITERVDFLRELSPLWEMVKEGIDLNSIEWSGH 497
+ EIDY I+ ++ +V FLR +SPLW++V+EG+DL++IEWS H
Sbjct: 457 EAEIDYAIENVSRQVSFLRNMSPLWDLVQEGVDLSTIEWSQH 498
>Hs20560255 [E] KOG1549 Cysteine desulfurase NFS1
Length = 457
Score = 554 bits (1428), Expect = e-157
Identities = 259/401 (64%), Positives = 331/401 (81%), Gaps = 1/401 (0%)
Query: 98 RPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNKEIEQARKYIADVIKA 157
RP+Y+D+QATTP DPRVLD ML + YGNPHS TH+YGWE+ +E+AR+ +A +I A
Sbjct: 57 RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116
Query: 158 DPKEIIFTSGATESNNMALKGVSRFYKKTRNHIITTRTEHKCVLEAARAMKNEGYEITFL 217
DP+EIIFTSGATESNN+A+KGV+RFY+ + H+ITT+TEHKCVL++ R+++ EG+++T+L
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176
Query: 218 NVDEQGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKEIGELCRKRKVFFHTDAAQA 277
V + G+I+L+ELEAAI+P+T LVSVM VNNEIGV QP+ EIG +C RKV+FHTDAAQA
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQA 236
Query: 278 YGKIPIDVNEMKIDLMSISSHKIYGPKGIGAIYVRRKPRVRLDPLISGGGQERGLRSGTL 337
GKIP+DVN+MKIDLMSIS HKIYGPKG+GAIY+RR+PRVR++ L SGGGQERG+RSGT+
Sbjct: 237 VGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 296
Query: 338 APPLVAGFGEAARLMMKEYENDSNHIKRLSDKLM-NALLSIDHTILNGSADHRYPGCVNI 396
PLV G G A + +E E D I +LS++L+ N + S+ ++NG H YPGC+N+
Sbjct: 297 PTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINL 356
Query: 397 SFAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDALAHSSIRFGIGRFTTD 456
SFAYVEGESLLMAL+D+ALSSGSACTSASLEPSYVL A+G D+ LAHSSIRFGIGRFTT+
Sbjct: 357 SFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTE 416
Query: 457 EEIDYVIKAITERVDFLRELSPLWEMVKEGIDLNSIEWSGH 497
EE+DY ++ + V LRE+SPLWEMV++GIDL SI+W+ H
Sbjct: 417 EEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH 457
>7297897 [E] KOG1549 Cysteine desulfurase NFS1
Length = 462
Score = 547 bits (1410), Expect = e-155
Identities = 269/435 (61%), Positives = 332/435 (75%), Gaps = 2/435 (0%)
Query: 65 EQANERAVSNPDVLDSGIHSLQHAYRESGEYGT-RPIYLDMQATTPTDPRVLDTMLKFYT 123
E + R + P Q + E RP+YLD QATTP DPRVLD ML + T
Sbjct: 28 EATSSRTAAKPAATSKEFRERQVRFNIKNEQTEGRPLYLDAQATTPMDPRVLDAMLPYLT 87
Query: 124 GLYGNPHSNTHSYGWETNKEIEQARKYIADVIKADPKEIIFTSGATESNNMALKGVSRFY 183
YGNPHS TH+YGWET +E+AR+ +A +I ADPKEIIFTSGATESNN+A+KGV+RFY
Sbjct: 88 NFYGNPHSRTHAYGWETESAVEKAREQVATLIGADPKEIIFTSGATESNNIAVKGVARFY 147
Query: 184 KKTRNHIITTRTEHKCVLEAARAMKNEGYEITFLNVDEQGLINLEELEAAIRPETCLVSV 243
+ H+ITT+TEHKCVL++ RA++NEG+++T+L V GLI+L++LE I ET LVS+
Sbjct: 148 GTKKRHVITTQTEHKCVLDSCRALENEGFKVTYLPVLANGLIDLQQLEETITSETSLVSI 207
Query: 244 MAVNNEIGVMQPLKEIGELCRKRKVFFHTDAAQAYGKIPIDVNEMKIDLMSISSHKIYGP 303
M VNNEIGV QP+ EIG+LCR R+VFFHTDAAQA GK+P+DVN M IDLMSIS HKIYGP
Sbjct: 208 MTVNNEIGVRQPVDEIGKLCRSRRVFFHTDAAQAVGKVPLDVNAMNIDLMSISGHKIYGP 267
Query: 304 KGIGAIYVRRKPRVRLDPLISGGGQERGLRSGTLAPPLVAGFGEAARLMMKEYENDSNHI 363
KG+GA+YVRR+PRVRL+P+ SGGGQERGLRSGT+ PL G G AA L ++E + D +
Sbjct: 268 KGVGALYVRRRPRVRLEPIQSGGGQERGLRSGTVPAPLAVGLGAAAELSLREMDYDKKWV 327
Query: 364 KRLSDKLMNALLS-IDHTILNGSADHRYPGCVNISFAYVEGESLLMALRDIALSSGSACT 422
LS++L++ + S + H I NG A Y GC+N+SFAYVEGESLLMAL+D+ALSSGSACT
Sbjct: 328 DFLSNRLLDRISSALPHVIRNGDAKATYNGCLNLSFAYVEGESLLMALKDVALSSGSACT 387
Query: 423 SASLEPSYVLHALGKDDALAHSSIRFGIGRFTTDEEIDYVIKAITERVDFLRELSPLWEM 482
SASLEPSYVL A+G D+ LAHSSIRFGIGRFTT EE+DY + V+ LRE+SPLWEM
Sbjct: 388 SASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTVEEVDYTADKCIKHVERLREMSPLWEM 447
Query: 483 VKEGIDLNSIEWSGH 497
V+EGIDL +I+WS H
Sbjct: 448 VQEGIDLKTIQWSQH 462
>CE17320 [E] KOG1549 Cysteine desulfurase NFS1
Length = 446
Score = 531 bits (1368), Expect = e-151
Identities = 262/436 (60%), Positives = 333/436 (76%), Gaps = 36/436 (8%)
Query: 98 RPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNKEIEQARKYIADVIKA 157
+PIYLD+QAT P DPRV+D ML + +GNPHS THSYGW+ + +EQAR+++A++IKA
Sbjct: 11 QPIYLDVQATAPMDPRVVDAMLPYMINDFGNPHSRTHSYGWKAEEGVEQAREHVANLIKA 70
Query: 158 DPKEIIFTSGATESNNMALKGVSRFYKKT-RNHIITTRTEHKCVLEAARAMKNEGYEITF 216
DP++IIFTSGATESNN+A+KGV++F K++ +NHIIT +TEHKCVL++ R ++NEG+++T+
Sbjct: 71 DPRDIIFTSGATESNNLAIKGVAKFRKQSGKNHIITLQTEHKCVLDSCRYLENEGFKVTY 130
Query: 217 LNVDEQGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKEIG---------------- 260
L VD+ G++++E+L +I ETCLVS+M VNNEIGVMQP+K+IG
Sbjct: 131 LPVDKGGMVDMEQLTQSITAETCLVSIMFVNNEIGVMQPIKQIGLFFFSNQRNTPKKKLN 190
Query: 261 ------------------ELCRKRKVFFHTDAAQAYGKIPIDVNEMKIDLMSISSHKIYG 302
ELCR + V+FHTDAAQA GK+PIDVNEMKIDLMSIS+HKIYG
Sbjct: 191 LTKISKFFSFDSLIAEFSELCRSKGVYFHTDAAQATGKVPIDVNEMKIDLMSISAHKIYG 250
Query: 303 PKGIGAIYVRRKPRVRLDPLISGGGQERGLRSGTLAPPLVAGFGEAARLMMKEYENDSNH 362
PKG GA+YVRR+PRVR++ +SGGGQERGLRSGT+A PL G GEAA++ KE D H
Sbjct: 251 PKGAGALYVRRRPRVRIEAQMSGGGQERGLRSGTVAAPLCIGLGEAAKIADKEMAMDKAH 310
Query: 363 IKRLSDKLMNALLS-IDHTILNGSADHRYPGCVNISFAYVEGESLLMALRDIALSSGSAC 421
++RLS L+N + + H I NG A H YPGCVN+SFAYVEGESLLMAL+ IALSSGSAC
Sbjct: 311 VERLSQMLINGISDKLPHIIRNGDARHAYPGCVNLSFAYVEGESLLMALKSIALSSGSAC 370
Query: 422 TSASLEPSYVLHALGKDDALAHSSIRFGIGRFTTDEEIDYVIKAITERVDFLRELSPLWE 481
TSASLEPSYVL A+G ++ LAHSSIRFG+GRFTTDEE+ + I + LR+LSPLWE
Sbjct: 371 TSASLEPSYVLRAIGSEEDLAHSSIRFGLGRFTTDEEVKHTIDLCIRETNRLRDLSPLWE 430
Query: 482 MVKEGIDLNSIEWSGH 497
MV+EGIDL SI+W+ H
Sbjct: 431 MVQEGIDLKSIQWTQH 446
>Hs10864079 [E] KOG1549 Cysteine desulfurase NFS1
Length = 457
Score = 520 bits (1340), Expect = e-147
Identities = 244/401 (60%), Positives = 318/401 (78%), Gaps = 1/401 (0%)
Query: 98 RPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNKEIEQARKYIADVIKA 157
RP+Y+D+QATTP DPRVLD ML + YGNPHS TH+YGWE+ +E+AR+ +A +I A
Sbjct: 57 RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116
Query: 158 DPKEIIFTSGATESNNMALKGVSRFYKKTRNHIITTRTEHKCVLEAARAMKNEGYEITFL 217
DP+EIIFTSGATESNN+A+KGV+RFY+ + H+ITT+TEHKCVL++ R+++ EG+++T+L
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176
Query: 218 NVDEQGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKEIGELCRKRKVFFHTDAAQA 277
V + G+I+L+ELEAAI+P+T LVSVM VNNEIGV QP+ EIG +C RKV+FHTDAAQA
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQA 236
Query: 278 YGKIPIDVNEMKIDLMSISSHKIYGPKGIGAIYVRRKPRVRLDPLISGGGQERGLRSGTL 337
GKIP+DVN+MKIDLMSIS HKIYGPKG+GAIY+RR+PRVR++ L SGGGQERG+RSGT+
Sbjct: 237 VGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTV 296
Query: 338 APPLVAGFGEAARLMMKEYENDSNHIKRLSDKLM-NALLSIDHTILNGSADHRYPGCVNI 396
PLV G G A + +E E D I +LS++L+ N + S+ ++NG H YPGC+N+
Sbjct: 297 PTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINL 356
Query: 397 SFAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDALAHSSIRFGIGRFTTD 456
SFAYVEGESLLMAL+D+ALSSGS+C+ P L L +HSSIRFGIG FTT+
Sbjct: 357 SFAYVEGESLLMALKDVALSSGSSCSLHPWSPLMCLEQLALMRIYSHSSIRFGIGAFTTE 416
Query: 457 EEIDYVIKAITERVDFLRELSPLWEMVKEGIDLNSIEWSGH 497
EE+DY ++ + V+ LRE+SPLWEMV++GIDL SI+W+ H
Sbjct: 417 EEVDYTVEKCIQHVNRLREMSPLWEMVQDGIDLKSIKWTQH 457
>At5g65720 [E] KOG1549 Cysteine desulfurase NFS1
Length = 453
Score = 517 bits (1331), Expect = e-146
Identities = 249/401 (62%), Positives = 316/401 (78%), Gaps = 1/401 (0%)
Query: 98 RPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNKEIEQARKYIADVIKA 157
RP+YLDMQATTP DPRV D M YGNPHS TH YGWE +E AR +A +I+A
Sbjct: 53 RPLYLDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEA 112
Query: 158 DPKEIIFTSGATESNNMALKGVSRFYKKTRNHIITTRTEHKCVLEAARAMKNEGYEITFL 217
PKEI+F SGATE+NNMA+KGV FYK T+ H+ITT+TEHKCVL++ R ++ EG+E+T+L
Sbjct: 113 SPKEIVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYL 172
Query: 218 NVDEQGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKEIGELCRKRKVFFHTDAAQA 277
V GL++LE L AIRP+T LVS+MAVNNEIGV+QP++EIG +C++ V FHTDAAQA
Sbjct: 173 PVKTDGLVDLEMLREAIRPDTGLVSIMAVNNEIGVVQPMEEIGMICKEHNVPFHTDAAQA 232
Query: 278 YGKIPIDVNEMKIDLMSISSHKIYGPKGIGAIYVRRKPRVRLDPLISGGGQERGLRSGTL 337
GKIP+DV + + LMS+S+HKIYGPKG+GA+YVRR+PR+RL+PL++GGGQERGLRSGT
Sbjct: 233 IGKIPVDVKKWNVALMSMSAHKIYGPKGVGALYVRRRPRIRLEPLMNGGGQERGLRSGTG 292
Query: 338 APPLVAGFGEAARLMMKEYENDSNHIKRLSDKLMNALL-SIDHTILNGSADHRYPGCVNI 396
A + GFG A L MKE E D IK L ++L+N + +D ++NGS D RY G +N+
Sbjct: 293 ATQQIVGFGAACELAMKEMEYDEKWIKGLQERLLNGVREKLDGVVVNGSMDSRYVGNLNL 352
Query: 397 SFAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDALAHSSIRFGIGRFTTD 456
SFAYVEGESLLM L+++A+SSGSACTSASLEPSYVL ALG D+ +AH+SIRFGIGRFTT
Sbjct: 353 SFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVDEDMAHTSIRFGIGRFTTK 412
Query: 457 EEIDYVIKAITERVDFLRELSPLWEMVKEGIDLNSIEWSGH 497
EEID ++ ++V+ LRE+SPL+EMVKEGID+ +I+WS H
Sbjct: 413 EEIDKAVELTVKQVEKLREMSPLYEMVKEGIDIKNIQWSQH 453
>ECU11g1770 [E] KOG1549 Cysteine desulfurase NFS1
Length = 432
Score = 508 bits (1308), Expect = e-144
Identities = 255/403 (63%), Positives = 314/403 (77%), Gaps = 4/403 (0%)
Query: 96 GTRPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNKEIEQARKYIADVI 155
G + I+LD+Q+TTP DPRVLD ML FYT ++GNPHS TH YGW+ +E+AR +A +I
Sbjct: 30 GGKRIFLDVQSTTPVDPRVLDAMLPFYTTVFGNPHSRTHRYGWQAEAAVEKARSQVASLI 89
Query: 156 KADPKEIIFTSGATESNNMALKGVSRFYKKTRN--HIITTRTEHKCVLEAARAMKNEGYE 213
DPKEIIFTSGATESNN+ALKGVS F K HIIT +TEHKC+L+ R ++ G E
Sbjct: 90 GCDPKEIIFTSGATESNNLALKGVSGFKLKEGKAAHIITLQTEHKCILDTCRNLEENGVE 149
Query: 214 ITFLNVDEQGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKEIGELCRKRKVFFHTD 273
+T+L V G++++++++ +I+ T LVS+ AVN+EIG +QPLKEIG LC++R V FHTD
Sbjct: 150 VTYLPVGNDGVVDIDDVKKSIKENTVLVSIGAVNSEIGTVQPLKEIGMLCKERGVLFHTD 209
Query: 274 AAQAYGKIPIDVNEMKIDLMSISSHKIYGPKGIGAIYVRRKPRVRLDPLISGGGQERGLR 333
AAQ GKI IDVNEM IDL+S+ +HKIYGPKGIGA+YVRR+PRVR+ PLI+GGGQERGLR
Sbjct: 210 AAQGVGKIQIDVNEMNIDLLSMCAHKIYGPKGIGALYVRRRPRVRMVPLINGGGQERGLR 269
Query: 334 SGTLAPPLVAGFGEAARLMMKEYENDSNHIKRLSDKLMNAL-LSIDHTILNGSADHRYPG 392
SGT+A PLV GFG+AA + KE + D HIK LS KL N +I+ I+NGS + +PG
Sbjct: 270 SGTVASPLVVGFGKAAEICSKEMKRDFEHIKELSKKLKNMFKKNIEGVIINGS-EKGFPG 328
Query: 393 CVNISFAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDALAHSSIRFGIGR 452
CVN+SF +VEGESLLM L+DIALSSGSACTSASLEPSYVL ALG+DD LAHSSIRFGIGR
Sbjct: 329 CVNVSFPFVEGESLLMHLKDIALSSGSACTSASLEPSYVLRALGRDDELAHSSIRFGIGR 388
Query: 453 FTTDEEIDYVIKAITERVDFLRELSPLWEMVKEGIDLNSIEWS 495
FT +EID V E V LRE+SPL+EMVKEGIDL+ I W+
Sbjct: 389 FTMAKEIDIVANKTVEAVQKLREMSPLYEMVKEGIDLSKISWT 431
>Hs19923803 [E] KOG1549 Cysteine desulfurase NFS1
Length = 445
Score = 201 bits (512), Expect = 2e-51
Identities = 137/416 (32%), Positives = 214/416 (50%), Gaps = 40/416 (9%)
Query: 98 RPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNKEIEQARKYIADVIKA 157
R +Y+D ATTP +P V+ M K +GNP S+ +S G + I AR+ +A +I
Sbjct: 30 RKVYMDYNATTPLEPEVIQAMTKAMWEAWGNP-SSPYSAGRKAKDIINAARESLAKMIGG 88
Query: 158 DPKEIIFTSGATESNNMALKGVSRFY-----------------KKTRNHIITTRTEHKCV 200
P++IIFTSG TESNN+ + V + + K + H IT+ EH +
Sbjct: 89 KPQDIIFTSGGTESNNLVIHSVVKHFHANQTSKGHTGGHHSPVKGAKPHFITSSVEHDSI 148
Query: 201 -LEAARAMKNEGYEITFLNVDE-QGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKE 258
L ++ + +TF+ V + G ++++ AA+RP T LV++M NNE G++ P+ E
Sbjct: 149 RLPLEHLVEEQVAAVTFVPVSKVSGQTEVDDILAAVRPTTRLVTIMLANNETGIVMPVPE 208
Query: 259 IGELCRKRK----------VFFHTDAAQAYGKIPIDVNEMKIDLMSISSHKIYGPKGIGA 308
I + + + HTDAAQA GK +DV ++ +D ++I HK YGP+ IGA
Sbjct: 209 ISQRIKALNQERVAAGLPPILVHTDAAQALGKQRVDVEDLGVDFLTIVGHKFYGPR-IGA 267
Query: 309 IYVRRKPRVR-LDPLISGGGQERGLRSGTLAPPLVAGFGEAARLMMKEYENDSNHIKRLS 367
+Y+R L P++ GGGQER R GT P++AG G+AA L+ + E H++ +
Sbjct: 268 LYIRGLGEFTPLYPMLFGGGQERNFRPGTENTPMIAGLGKAAELVTQNCEAYEAHMRDVR 327
Query: 368 DKL---MNALLSIDHTILNGS--ADHRYPGCVNISF--AYVEGESLLMALRDIALSSGSA 420
D L + A LN R P N S ++G +L R + S G+A
Sbjct: 328 DYLEERLEAEFGQKRIHLNSQFPGTQRLPNTCNFSIRGPRLQGHVVLAQCRVLMASVGAA 387
Query: 421 CTS-ASLEPSYVLHALGKDDALAHSSIRFGIGRFTTDEEIDYVIKAITERVDFLRE 475
C S +PS VL + G +A +++R +GR TT E+D V++ + + V L +
Sbjct: 388 CHSDHGDQPSPVLLSYGVPFDVARNALRLSVGRSTTRAEVDLVVQDLKQAVAQLED 443
>CE02643 [E] KOG1549 Cysteine desulfurase NFS1
Length = 328
Score = 169 bits (428), Expect = 9e-42
Identities = 106/327 (32%), Positives = 175/327 (53%), Gaps = 16/327 (4%)
Query: 154 VIKADPKEIIFTSGATESNNMALKGVSRFYKKTRN--HIITTRTEHKCVLEAARAMKNEG 211
+ D + ++FTSG TESNN ++G R KK HIITT EH +LE + + +G
Sbjct: 1 MFNVDGECVVFTSGGTESNNWVIEGTIRNAKKVSKLPHIITTNIEHPSILEPLKRREEDG 60
Query: 212 -YEITFLNVDE-QGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKEIGELCRKRKV- 268
+T+++++ G + + + A+ +TCLV++M NN+ GV+QP+ EI + R++
Sbjct: 61 EISVTYVSINPLTGFVTSQSILDALTSDTCLVTIMLANNDTGVLQPVSEIFQAIREKLKT 120
Query: 269 ---FFHTDAAQAYGKIPIDVNEMKIDLMSISSHKIYGPKGIGAIYVRRKPRVRLDPLISG 325
F H+D AQA GKIP++V + D +++ HK YGP+ GA+ K + R+ P++ G
Sbjct: 121 NVPFLHSDVAQAAGKIPVNVRSLSADAVTVVGHKFYGPRS-GALIFNPKSK-RIPPMLLG 178
Query: 326 GGQERGLRSGTLAPPLVAGFGEAARLMMKEYENDSNHIKRLSDKLMNALLS--IDHTILN 383
G QE G RSGT P++ G GEAA++ + + N + +++ D LL + I++
Sbjct: 179 GNQESGWRSGTENTPMIVGLGEAAKVYNEGFLNIESILRQNRDYFEELLLKRLRNSHIIH 238
Query: 384 GSADHRYPGCVNISFAYV--EGESLLMALRDIALSSGSACTSASLEPSYVLHALGKDDAL 441
R P +++F G LL + S+G+AC P +L A G A+
Sbjct: 239 FIGSQRLPNTSSVAFLDYPSHGCDLLEKCQTFNASTGAACHKNECSP--ILKACGIPFAV 296
Query: 442 AHSSIRFGIGRFTTDEEIDYVIKAITE 468
A ++R GR T EI+ V+ + +
Sbjct: 297 ASKTVRISFGRSTKIAEIETVVDELVQ 323
>At1g08490 [E] KOG1549 Cysteine desulfurase NFS1
Length = 503
Score = 108 bits (271), Expect = 1e-23
Identities = 70/233 (30%), Positives = 112/233 (48%), Gaps = 2/233 (0%)
Query: 96 GTRPIYLDMQATTPTDPRVLDTMLKFYTGLYGNPHSNTHSYGWETNKEIEQARKYIADVI 155
G++ +YLD AT+ VLD + +Y N H H + E E ARK +A I
Sbjct: 70 GSKLVYLDSAATSQKPAAVLDALQNYYEFYNSNVHRGIHYLSAKATDEFELARKKVARFI 129
Query: 156 KA-DPKEIIFTSGATESNNMALKGVSRFYKKTRNHIITTRTEH-KCVLEAARAMKNEGYE 213
A D +EI+FT ATE+ N+ K + +I T EH C++ + G
Sbjct: 130 NASDSREIVFTRNATEAINLVAYSWGLSNLKPGDEVILTVAEHHSCIVPWQIVSQKTGAV 189
Query: 214 ITFLNVDEQGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKEIGELCRKRKVFFHTD 273
+ F+ ++E + ++ +L I P+T LV+V V+N + P++EI D
Sbjct: 190 LKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNVLASSLPIEEIVVWAHDVGAKVLVD 249
Query: 274 AAQAYGKIPIDVNEMKIDLMSISSHKIYGPKGIGAIYVRRKPRVRLDPLISGG 326
A Q+ + +DV ++ D + SSHK+ GP GIG +Y + + P + GG
Sbjct: 250 ACQSVPHMVVDVQKLNADFLVASSHKMCGPTGIGFLYGKSDLLHSMPPFLGGG 302
>SPAC11D3.10 [E] KOG1549 Cysteine desulfurase NFS1
Length = 434
Score = 63.9 bits (154), Expect = 5e-10
Identities = 61/237 (25%), Positives = 102/237 (42%), Gaps = 16/237 (6%)
Query: 97 TRPIYLDMQATTPTDPRVLDTMLKFYT--GLYGNPHSNTHSYGWETNKEIEQARKYIADV 154
T+ IYL+ P + ++ T L+ Y GLY NPH W+ + E+ R +A +
Sbjct: 21 TKTIYLNQSFQAPMN-MLVSTALQGYINEGLY-NPHPKPM---WK--ERTEETRSLLAKL 73
Query: 155 IKADPKE-IIFTSGATESNNMALKGVSRFYKKTRNHIITTRTEHKCVLEAARAMKNEGYE 213
+ A K+ I FT TE N+ + + K ++++ EH A++N+G E
Sbjct: 74 LNASTKDSITFTRDTTEGLNLFQRSLKW---KPGDNVVILDNEHPNQGFGWIALQNDGLE 130
Query: 214 ITFLNVDEQGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKEIGELCRKRKVFFHTD 273
+ + + Q N + T + + +V G +K+I R + + D
Sbjct: 131 VRLVPNEGQYHANASTFAPYVDSRTKAIGLSSVMFHSGQKNDVKDIANAFRPKGIHVLAD 190
Query: 274 AAQAYGKIPIDVNEMKIDLMSISSHKIYG-PKGIGAIYVR--RKPRVRLDPLISGGG 327
Q G IDV ++ + + S HK G P G+G +YV +R P GGG
Sbjct: 191 LTQQVGLSKIDVQDLNVSACAFSCHKGLGCPTGLGVLYVSPLAISELRSTPPFVGGG 247
>Hs8923661 [H] KOG2142 Molybdenum cofactor sulfurase
Length = 888
Score = 56.6 bits (135), Expect = 8e-08
Identities = 69/257 (26%), Positives = 105/257 (40%), Gaps = 26/257 (10%)
Query: 100 IYLDMQATTPTDPRVLDTMLK-FYTGLYGNPHSNTHSYGWETNKEIEQARKYIADVIK-- 156
+YLD T L++ YGNPHS S T+ +EQ R I
Sbjct: 50 VYLDHAGATLFSQSQLESFTSDLMENTYGNPHSQNISSKL-THDTVEQVRYRILAHFHTT 108
Query: 157 ADPKEIIFTSGATESNNM---ALKGVSRFYKKTRNHIITTRTEHKCVLEAARAMKNEGYE 213
A+ +IFT+G+T + + A VS+ + + + H V+ M+N
Sbjct: 109 AEDYTVIFTAGSTAALKLVAEAFPWVSQGPESSGSRFCYLTDSHTSVV----GMRNVTMA 164
Query: 214 ITFLNVD--EQGLINLEELEAAIRPETC----LVSVMAVNNEIGVMQPLKEIGELCRKR- 266
I +++ + L + EE A+ C L A +N GV PL I E+ R
Sbjct: 165 INVISIPVRPEDLWSAEERGASASNPDCQLPHLFCYPAQSNFSGVRYPLSWIEEVKSGRL 224
Query: 267 -------KVFFHTDAAQAYGKIPIDVNEMKIDLMSISSHKIYG-PKGIGAIYVRRKPRVR 318
K F DAA P+D++ + D + IS +KI+G P G+GA+ V +
Sbjct: 225 RPVSTPGKWFVLLDAASYVSTSPLDLSAHQADFVPISFYKIFGFPTGLGALLVHNRAAPL 284
Query: 319 LDPLISGGGQERGLRSG 335
L GGG +G
Sbjct: 285 LRKTYFGGGTASAYLAG 301
>7297896 [E] KOG1549 Cysteine desulfurase NFS1
Length = 134
Score = 51.6 bits (122), Expect = 3e-06
Identities = 27/65 (41%), Positives = 32/65 (48%), Gaps = 1/65 (1%)
Query: 65 EQANERAVSNPDVLDSGIHSLQHAYRESGEYGT-RPIYLDMQATTPTDPRVLDTMLKFYT 123
E + R + P Q + E RP+YLD QATTP DPRVLD ML + T
Sbjct: 28 EATSSRTAAKPAATSKEFRERQVRFNIKNEQTEGRPLYLDAQATTPMDPRVLDAMLPYLT 87
Query: 124 GLYGN 128
YGN
Sbjct: 88 NFYGN 92
>SPCC777.03c [E] KOG1549 Cysteine desulfurase NFS1
Length = 396
Score = 51.2 bits (121), Expect = 3e-06
Identities = 66/312 (21%), Positives = 127/312 (40%), Gaps = 35/312 (11%)
Query: 101 YLDMQATTPTDPRVLDTMLKFYT-GLYGNPHSNTHSYGWETNKEIEQARKYIADVIKADP 159
+ ++ P + V +T+ ++ GLY NP+ W+ ++ E+ R+ +A + A
Sbjct: 28 FFNLSFQPPMNSIVANTINEYVDWGLY-NPNPKPL---WQ--QKAEETRELVAKYLNASS 81
Query: 160 KE-IIFTSGATESNNMALKGVSRFYKKTRNHIITTRTEHKCVLEAARAMKNEGYEITFLN 218
+ I FT TE N+ + + + + ++++ EH +++ G E+ +
Sbjct: 82 SDSIAFTRDTTEGLNLFQRSM---HFQPGDNVVLLDGEHPNHGFGWISLQEAGLEVRLIP 138
Query: 219 VDEQGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKEIGELCRKRKVFFHTDAAQAY 278
+ + E + +T + + +V G + +K+I R + D Q
Sbjct: 139 SKDIYYADASTFEPYVDEKTKAIGISSVMFHSGQLNNVKDICNKFRPENIHVLVDMTQQV 198
Query: 279 GKIPIDVNEMKIDLMSISSHK-IYGPKGIGAIYVRRK--PRVRLDPLISGGGQERGLRSG 335
G IDV E+ + + + HK + P G+G +YV P+++ P I G G L +
Sbjct: 199 GLSSIDVQELNVSACAFACHKGLSCPTGLGVLYVAPDVLPQLKNVPPIVGAGAIENLDAN 258
Query: 336 TLAPPLVAGFGEAAR--------------------LMMKEYENDSNHIKRLSDKLMNALL 375
L F A R LM +EN +++ L L++ L
Sbjct: 259 LLVNLNPIYFSTARRFEHLNKSLITTLCLNNYLSFLMDIGFENIEQYLRELGKSLVDELS 318
Query: 376 SIDHTILNGSAD 387
+ TI+ GS D
Sbjct: 319 KLGVTII-GSKD 329
>At1g27980 [E] KOG1383 Glutamate decarboxylase/sphingosine phosphate lyase
Length = 544
Score = 50.1 bits (118), Expect = 8e-06
Identities = 62/245 (25%), Positives = 101/245 (40%), Gaps = 26/245 (10%)
Query: 165 TSGATESNNMALKGVSRF--YKK--TRNHIITTRTEHKCVLEAARAMKNEGYEITFLNVD 220
TSG TES +A+K + YKK TR +I + H +AA+ K + + + VD
Sbjct: 203 TSGGTESIVLAVKSSRDYMKYKKGITRPEMIIPESGHSAYDKAAQYFKIKLWRVP---VD 259
Query: 221 EQGLINLEELEAAIRPETCLVSVMAVNNEIGVMQPLKEIGELCRKRKVFFHTDAA----- 275
+ +++ I T ++ A G++ P++E+G+L + FH D
Sbjct: 260 KDFRADVKATRRHINRNTIMIVGSAPGFPHGIIDPIEELGQLALSYGICFHVDLCLGGFV 319
Query: 276 ------QAYGKIPIDVNEMKIDLMSISSHKIYG--PKGIGAIYVR----RKPRVRLDPLI 323
Y P D + + +S+ HK YG PKG + R RK +
Sbjct: 320 LPFARKLGYQIPPFDFSVQGVTSISVDVHK-YGLAPKGTSTVLYRNHEIRKHQFVAVTEW 378
Query: 324 SGGGQERGLRSGTLAPPLVAGFGEAARLMMKE-YENDSNHIKRLSDKLMNALLSIDHTIL 382
SGG +G+ LVAG A + +E Y +++ I S +L + I +
Sbjct: 379 SGGLYVSPTIAGSRPGSLVAGAWAAMMSLGEEGYLQNTSKIMEASKRLEEGVREIHELFV 438
Query: 383 NGSAD 387
G D
Sbjct: 439 IGKPD 443
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,743,412
Number of Sequences: 60738
Number of extensions: 1201555
Number of successful extensions: 3061
Number of sequences better than 1.0e-05: 16
Number of HSP's better than 0.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 3029
Number of HSP's gapped (non-prelim): 17
length of query: 497
length of database: 30,389,216
effective HSP length: 111
effective length of query: 386
effective length of database: 23,647,298
effective search space: 9127857028
effective search space used: 9127857028
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)