ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV1436 good TO KOG3288 Posttranslational modification, protein turnover, chaperones OTU-like cysteine protease
r_klactIV1436 good TO KOG3288 Signal transduction mechanisms OTU-like cysteine protease
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV1436 485753 486709 319
(319 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YFL044c [TO] KOG3288 OTU-like cysteine protease 237 2e-62
SPAC24C9.14 [TO] KOG3288 OTU-like cysteine protease 147 2e-35
7295482 [TO] KOG3288 OTU-like cysteine protease 134 2e-31
Hs18545764 [TO] KOG3288 OTU-like cysteine protease 131 1e-30
At1g50670 [TO] KOG3288 OTU-like cysteine protease 125 6e-29
>YFL044c [TO] KOG3288 OTU-like cysteine protease
Length = 301
Score = 237 bits (604), Expect = 2e-62
Identities = 130/324 (40%), Positives = 203/324 (62%), Gaps = 31/324 (9%)
Query: 1 MRVKVSISGKSDKVLTVEAECDLETFQGLILSSYEDDLSLQSMRYGFPPQSILFQGNEAK 60
M++KV+ +G ++V+T++ + L I ++ +++MR+G+PPQ I QG +A
Sbjct: 1 MKLKVTGAG-INQVVTLKQDATLNDLIEHI------NVDVKTMRFGYPPQRINLQGEDAS 53
Query: 61 T----LEELGICNGERIIVSLSKADEAIATRTQPSINASKANTLPNKEVKKKPTGKSESV 116
L+ELGI +GE+I + S ++E+ + LP + K K KS +
Sbjct: 54 LGQTQLDELGINSGEKITIESSDSNESFS--------------LPPPQPKPKRVLKSTEM 99
Query: 117 QIDST--RALQLHEVPDDNSCLFHAISYAVYKNISLSSELRGVVASEVRRDPFEYSDAIL 174
I + L +H V DDNSCLFHAI+Y ++K S+ +LR +V+ EV +P +++DAIL
Sbjct: 100 SIGGSGENVLSVHPVLDDNSCLFHAIAYGIFKQDSVR-DLREMVSKEVLNNPVKFNDAIL 158
Query: 175 GRPNHEYSEWIMKHSSWGGGIEIAILSKHFQYAIYVLDVDARKFEKFNEDLYDKFIIIAY 234
+PN +Y++WI+K SWGG IEI I+S AIYV+D+DA K EKFNED +D +I+I +
Sbjct: 159 DKPNKDYAQWILKMESWGGAIEIGIISDALAVAIYVVDIDAVKIEKFNEDKFDNYILILF 218
Query: 235 NGVHYDTVEIFDGTQHQTVFDIGEKDTEFLLQKTQEIAKKMKREGKSFNTARDSILCNTC 294
NG+HYD++ + + +TVF+ + +++ +L ++A +K+ G SFNT + I CNTC
Sbjct: 219 NGIHYDSLTM---NEFKTVFNKNQPESDDVLTAALQLASNLKQTGYSFNTHKAQIKCNTC 275
Query: 295 GTTLIGEREVSKHAEKTGHVDFRQ 318
T +GEREV++HAE TGHVDF Q
Sbjct: 276 QMTFVGEREVARHAESTGHVDFGQ 299
>SPAC24C9.14 [TO] KOG3288 OTU-like cysteine protease
Length = 329
Score = 147 bits (371), Expect = 2e-35
Identities = 107/334 (32%), Positives = 170/334 (50%), Gaps = 29/334 (8%)
Query: 1 MRVKVSISGKSDKVLTVEAECDLETFQGLILSSYEDDLSLQSMRYGFPPQSILFQGNEAK 60
+R+++ +S V TVEA + +F L+ + + + ++++GFPPQ I ++
Sbjct: 4 LRLRLKYENQS-AVETVEANATVGSFLDLVAAKFSLPRNSIALKFGFPPQDIPLVNSDVP 62
Query: 61 TLEELGICNGERIIVSLSKADEAIATR--TQPSIN--ASKANTLPNKEVKKKPTGKSESV 116
L L + +G++I+V L A + +T +P I A+K P E+ P +S
Sbjct: 63 -LSTL-VSSGQQILV-LKNAATSFSTNEPAKPPIPNAATKPTFPPQTEISNPPAVSHQSK 119
Query: 117 QIDSTRA--------LQLHEVPDDNSCLFHAISYAVYKNISLSS-ELRGVVASEVRRDPF 167
+ L +PDDNSCLF A+S K + S ELR +VA++V +P
Sbjct: 120 NTSQDPPYVSTPIGDIALRVMPDDNSCLFRALS----KPLGFSPYELREIVANQVLSNPD 175
Query: 168 EYSDAILGRPNHEYSEWIMKHSSWGGGIEIAILSKHFQYAIYVLDVDARKFEKFN-EDLY 226
YS AILG+P+ EY+ WI K +SWGG IE++ILS HF I +DV + + +N +
Sbjct: 176 IYSTAILGKPSIEYASWIRKETSWGGYIELSILSSHFGVEICSVDVKTGRVDSYNPQPAT 235
Query: 227 DKFIIIAYNGVHYD----TVEIFDGTQHQTVFDIGEKDTEFLLQKTQEIAKKMKREGKSF 282
+ I Y+G+HYD ++D +FD + + Q++A +K
Sbjct: 236 GQRTYIVYSGIHYDLAALAAVLWDTDVDVVLFDASDVT---ITPYVQQLASLLKNMHYYT 292
Query: 283 NTARDSILCNTCGTTLIGEREVSKHAEKTGHVDF 316
+TA SI C CGT L+GE++ + HA TGH F
Sbjct: 293 DTASFSIRCTICGTGLVGEKDATAHALATGHTQF 326
>7295482 [TO] KOG3288 OTU-like cysteine protease
Length = 347
Score = 134 bits (336), Expect = 2e-31
Identities = 89/298 (29%), Positives = 146/298 (48%), Gaps = 30/298 (10%)
Query: 46 GFPPQSI-LFQGNEAKTLEELGICNGERIIVSLSKA---------------DEAIATRTQ 89
G+PP+ + L Q E + L+ +GI +GE +IV A DEA+A R Q
Sbjct: 50 GYPPKPLDLSQQQEQRALKAVGINSGETLIVEEKAAPAPAAPVPGGTTVEDDEALARRLQ 109
Query: 90 PSINASKANTLPNKEVKKK-----PTGKSESVQIDSTRALQLHEV-PDDNSCLFHAISYA 143
A V + P +ES + L +V P DNSCLF +I +
Sbjct: 110 AEEEAQLLQETAGGPVAQAADYQLPVAPTESGPNGDFNGILLKKVVPADNSCLFTSIRFV 169
Query: 144 VYKNISL--SSELRGVVASEVRRDPFEYSDAILGRPNHEYSEWIMKHSSWGGGIEIAILS 201
+ + S +R ++A EV DP Y+DA+LG+ N EY WI K SWGG IE++ILS
Sbjct: 170 LNGKVDNEGSEMMRHIIAQEVAADPQSYNDAVLGKSNAEYCAWIQKADSWGGAIEVSILS 229
Query: 202 KHFQYAIYVLDVDARKFEKFNEDL-YDKFIIIAYNGVHYDT--VEIFDGTQHQTVFDIGE 258
++ I V+D+ +F ED + + + ++G+HYD +E T+F + E
Sbjct: 230 NYYGIEIDVVDIQNAIINRFGEDKNFGLRVFLLFDGIHYDPLYMETSPSAAPATIFPVEE 289
Query: 259 KDTEFLLQKTQEIAKKMKREGKSFNTARDSILCNTCGTTLIGEREVSKHAEKTGHVDF 316
+ Q+ +++A + + + N + ++ C C L+G+ + +HA++TGH +F
Sbjct: 290 LG---VYQQAEQLANEAQSSRQYTNVDKFTLRCMQCDVRLVGQVQAQEHAKQTGHKNF 344
>Hs18545764 [TO] KOG3288 OTU-like cysteine protease
Length = 232
Score = 131 bits (330), Expect = 1e-30
Identities = 69/192 (35%), Positives = 110/192 (56%), Gaps = 4/192 (2%)
Query: 129 VPDDNSCLFHAISYAV---YKNISLSSELRGVVASEVRRDPFEYSDAILGRPNHEYSEWI 185
VP DNSCLF ++ Y V N + + E+R ++A V DP YS+AILG+ N EY +WI
Sbjct: 38 VPADNSCLFTSVYYVVEGGVLNPACAPEMRRLIAQIVASDPDFYSEAILGKTNQEYCDWI 97
Query: 186 MKHSSWGGGIEIAILSKHFQYAIYVLDVDARKFEKFNEDL-YDKFIIIAYNGVHYDTVEI 244
+ +WGG IEI+ILSK +Q I V+D + ++F ED Y K +++ Y+G+HYD ++
Sbjct: 98 KRDDTWGGAIEISILSKFYQCEICVVDTQTVRIDRFGEDAGYTKRVLLIYDGIHYDPLQR 157
Query: 245 FDGTQHQTVFDIGEKDTEFLLQKTQEIAKKMKREGKSFNTARDSILCNTCGTTLIGEREV 304
I + + +L + E+A + +R + + R ++ C C L G+ E
Sbjct: 158 NFPDPDTPPLTIFSSNDDIVLVQALELADEARRRRQFTDVNRFTLRCMVCQKGLTGQAEA 217
Query: 305 SKHAEKTGHVDF 316
+HA++TGH +F
Sbjct: 218 REHAKETGHTNF 229
>At1g50670 [TO] KOG3288 OTU-like cysteine protease
Length = 208
Score = 125 bits (315), Expect = 6e-29
Identities = 62/197 (31%), Positives = 116/197 (58%), Gaps = 7/197 (3%)
Query: 129 VPDDNSCLFHAISYAVYKNISLSSELRGVVASEVRRDPFEYSDAILGRPNHEYSEWIMKH 188
+P DNSCLF+AI Y + K+ + + ELR V+A+ V + +Y++A LG+ N EY WI+
Sbjct: 10 IPSDNSCLFNAIGYVMDKDKNKAPELRQVIAAAVASNKEKYNEAFLGKLNEEYCAWILNP 69
Query: 189 SSWGGGIEIAILSKHFQYAIYVLDVDARKFEKFNEDL-YDKFIIIAYNGVHYDTVEI--F 245
WGG IE++IL+ ++ I D+ + + + + YD+ +++ Y+G+HYD + + F
Sbjct: 70 DKWGGAIELSILADYYGREIAAYDIQTSRCDLYGQTRNYDERVMLIYDGLHYDALALSPF 129
Query: 246 DGTQH---QTVFDIG-EKDTEFLLQKTQEIAKKMKREGKSFNTARDSILCNTCGTTLIGE 301
+G + T++ +G ++ + + K +R+ +TA ++ C C +IG+
Sbjct: 130 EGAEEDFDMTIYPVGKDRSIGSIEGLALNLVKDQQRKRSYTDTANFTLRCGVCQIGVIGQ 189
Query: 302 REVSKHAEKTGHVDFRQ 318
+E +HA+ TGHV+F++
Sbjct: 190 KEAVEHAQATGHVNFQE 206
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,692,063
Number of Sequences: 60738
Number of extensions: 778371
Number of successful extensions: 2567
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2546
Number of HSP's gapped (non-prelim): 5
length of query: 319
length of database: 30,389,216
effective HSP length: 106
effective length of query: 213
effective length of database: 23,950,988
effective search space: 5101560444
effective search space used: 5101560444
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)