ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV1510 good R KOG2730 General function prediction only Methylase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV1510 514645 513737 -303
(303 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL157w [R] KOG2730 Methylase 272 5e-73
Hs19923661 [R] KOG2730 Methylase 125 1e-28
CE23958 [R] KOG2730 Methylase 107 2e-23
At1g30550 [R] KOG2730 Methylase 105 8e-23
SPAC521.01 [R] KOG2730 Methylase 59 7e-09
>YPL157w [R] KOG2730 Methylase
Length = 315
Score = 272 bits (695), Expect = 5e-73
Identities = 123/236 (52%), Positives = 168/236 (71%), Gaps = 2/236 (0%)
Query: 35 DDSYRINPYQRPNDKKLFKFWKNRATIFKLINNGNIYLTDELWFSVTPENLAQFTANYVR 94
+D+Y+I + + KLFK+WKNR +F I++ +IY+TDELWFSVTPE +A F AN+V+
Sbjct: 33 NDAYKIESSKPLKNGKLFKYWKNRRRLFSKIDSASIYMTDELWFSVTPERIACFLANFVK 92
Query: 95 HCLPNGNCIVDVFCGGGGNTIQFARLFEKVYGIDSNIDHLYCAYKNAECYNSAVKLKLIY 154
C+PN I+DVFCGGGGNTIQFA F VYG+D +I+H+YC KNA+ Y ++ L
Sbjct: 93 ACMPNAERILDVFCGGGGNTIQFAMQFPYVYGVDYSIEHIYCTAKNAQSYGVDDRIWLKR 152
Query: 155 GDWEESLKKTLVKNLGKTPDCIFASPPWGGVDYLRQDTYDLEEHLQPSPLSKILSVFFSV 214
G W++ + K + + DC+F SPPWGG +YLR D YDLE+HL+P ++K+L F +
Sbjct: 153 GSWKKLVSKQKLSKI--KYDCVFGSPPWGGPEYLRNDVYDLEQHLKPMGITKMLKSFLKL 210
Query: 215 CQNVVLFLPRNSDLDQLSSITHDLLGPDEKCKVVYVKDNGYMKGILAFWGRPFYDF 270
NV++FLPRNSDL+QLS T +LGP KCKV+YVK+NGYMKGI WG F+++
Sbjct: 211 SPNVIMFLPRNSDLNQLSRATRKVLGPFAKCKVLYVKENGYMKGIFCMWGECFFNY 266
>Hs19923661 [R] KOG2730 Methylase
Length = 853
Score = 125 bits (313), Expect = 1e-28
Identities = 80/215 (37%), Positives = 116/215 (53%), Gaps = 14/215 (6%)
Query: 50 KLFKFWKNRATIFKLINNGNIYLTDELWFSVTPENLAQFTANYVRHCLPNGNCIVDVFCG 109
+L K+W R +F ++G I L E WFSVTPE +A+ A V + +VD FCG
Sbjct: 643 ELAKYWAQRYRLFSRFDDG-IKLDREGWFSVTPEKIAEHIAGRVSQSF-KCDVVVDAFCG 700
Query: 110 GGGNTIQFARLFEKVYGIDSNIDHLYCAYKNAECYNSAVKLKLIYGDWEESLKKTLVKNL 169
GGNTIQFA +V ID + + A NAE Y A K++ I GD+ L + +K
Sbjct: 701 VGGNTIQFALTGMRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDF--LLLASFLK-- 756
Query: 170 GKTPDCIFASPPWGGVDYLRQDTYDLEEHLQPSPLSKILSVFFSVCQNVVLFLPRNSDLD 229
D +F SPPWGG DY +T+D+ + P +I + + N+V FLPRN+D+D
Sbjct: 757 ---ADVVFLSPPWGGPDYATAETFDIRTMMSPDGF-EIFRLSKKITNNIVYFLPRNADID 812
Query: 230 QLSSITHDLLGPDEKCKVVYVKDNGYMKGILAFWG 264
Q++S L GP + ++ N +K I A++G
Sbjct: 813 QVAS----LAGPGGQVEIEQNFLNNKLKTITAYFG 843
>CE23958 [R] KOG2730 Methylase
Length = 566
Score = 107 bits (267), Expect = 2e-23
Identities = 72/223 (32%), Positives = 108/223 (48%), Gaps = 8/223 (3%)
Query: 47 NDKKLFKFWKNRATIFKLINNGNIYLTDELWFSVTPENLAQFTANYVRHCLPNGNCIVDV 106
ND ++ K++ R +F ++ G I + E WFSVTPE +A+ A+ V N + +VD
Sbjct: 341 NDPEISKYYYQRYRLFSRLDQG-IIMDREGWFSVTPEAIAEHIADRVVR--NNVSVVVDA 397
Query: 107 FCGGGGNTIQFARLFEKVYGIDSNIDHLYCAYKNAECYNSAVKLKLIYGD-WEESLKKTL 165
F G GGN IQFA V ID + L CA +NA Y + I D ++ +
Sbjct: 398 FTGVGGNAIQFALRGAHVIAIDMDPVRLKCARENARVYGVENYIDFICADFFDVAATWQA 457
Query: 166 VKNLGKTPDCIFASPPWGGVDYLRQDTYDLEEHLQPSPLSKILSVFFSVCQNVVLFLPRN 225
K L D +F SPPWGG YL+ +DL P+ + I V +C N+ FLPRN
Sbjct: 458 DKKLAPKVDAVFLSPPWGGPSYLKAKEFDLATGCCPNGID-IFDVSLKICPNIAYFLPRN 516
Query: 226 SDLDQLSSITHDLLGPDEKCKVVYVKDNGYMKGILAFWGRPFY 268
+ + QL + + ++ N +K I ++G+ Y
Sbjct: 517 TKVSQLVELA---TKAKSRMEIEQSALNSKIKTITVYYGKLAY 556
>At1g30550 [R] KOG2730 Methylase
Length = 398
Score = 105 bits (262), Expect = 8e-23
Identities = 72/212 (33%), Positives = 108/212 (49%), Gaps = 18/212 (8%)
Query: 23 IKVRHRLKKHFFDDSYRINPYQRPNDKKLFKFWKNRATIFKLINNGNIYLTDELWFSVTP 82
+K R RLKK + N + K+ K+W R +F + G I + +E W+SVTP
Sbjct: 165 LKKRSRLKKEVKSTIEKDNGRRH----KITKYWIQRYDLFSRYDQG-IEMDEEGWYSVTP 219
Query: 83 ENLAQFTANYVRHCLPNGNCIVDVFCGGGGNTIQFARLFEKVYGIDSNIDHLYCAYKNAE 142
E +A A R G ++D F G GGNTIQFA++ V ID + + A NA
Sbjct: 220 EEIAIKQAQRYR-----GKVVIDCFSGVGGNTIQFAKVCSSVVAIDIDPVKVELAMNNAM 274
Query: 143 CYNSAVKLKLIYGDWEESLKKTLVKNLGKTPDCIFASPPWGGVDYLRQDTYDLEEHLQPS 202
Y A ++ + GD+ + L +L D +F SPPWGG Y ++Y+L + LQP
Sbjct: 275 VYGVANRVDFVIGDFIQ-----LAPSL--KGDVVFLSPPWGGPMYRDFESYNL-DMLQPR 326
Query: 203 PLSKILSVFFSVCQNVVLFLPRNSDLDQLSSI 234
+ + S+ N+++FLPRN DL Q+ +
Sbjct: 327 DGYSLFQIAQSITPNIIMFLPRNVDLAQVEEL 358
Score = 64.7 bits (156), Expect = 2e-10
Identities = 56/226 (24%), Positives = 91/226 (39%), Gaps = 62/226 (27%)
Query: 50 KLFKFWKNRATIFKLINNGNIYLTDELWFSVTPENLAQFTANYVRHCLPNGNCIVDVFCG 109
K+ ++W R +F + G I + +E W+SVTPE +A A R G ++D F G
Sbjct: 18 KISRYWIQRYDLFSKYDQG-IEMDEEGWYSVTPEEIAIKQAERCR-----GKVVIDCFSG 71
Query: 110 GGGNTIQFARLFEKVYGIDSNIDHLYCAYKNAECYNSAVKLKLIYGDWEESLKKTLVKNL 169
G ID + + A NA+ Y A ++ + GD+ + L +L
Sbjct: 72 VGA--------------IDIDPMKIALAMNNAKVYGVANRIDFVTGDFMQ-----LAPSL 112
Query: 170 GKTPDCIFASPPWGGVDYLRQDTYDLEEHLQPSPLSKILSVFFSVCQNVVLFLPRNSDLD 229
D +F SPPWGG Y + ++Y L+ + LPR+ ++
Sbjct: 113 --KGDVLFLSPPWGGPTYSKVESYKLD-----------------------MLLPRDGGVE 147
Query: 230 QLSSITHDLLGPDEKCKV------------VYVKDNGYMKGILAFW 263
++ S+TH+ K+ KDNG I +W
Sbjct: 148 RVDSLTHESHNMTRSMKLKKRSRLKKEVKSTIEKDNGRRHKITKYW 193
>SPAC521.01 [R] KOG2730 Methylase
Length = 135
Score = 59.3 bits (142), Expect = 7e-09
Identities = 42/118 (35%), Positives = 61/118 (51%), Gaps = 4/118 (3%)
Query: 46 PNDKKLFKFWKNRATIFKLINNGNIYLTDELWFSVTPENLAQFTANYVRHCLPNGNCIVD 105
P K L K+W NR +F + G I+L + W+SVTPE +A A V + I+D
Sbjct: 21 PVPKALKKYWNNRYNLFSRFDEG-IWLDYQSWYSVTPEKVAVAIAKSVVDFI-QPELIID 78
Query: 106 VFCGGGGNTIQFARLFEKVYGIDSNIDHLYCAYKNAECYN-SAVKLKLIYGDWEESLK 162
F G GGNTIQFA+ + V I+ + + A N E Y + ++ I GD ++ K
Sbjct: 79 AFSGCGGNTIQFAK-YCPVISIEIDPIKIAMAKHNLEIYGIPSSRVTFIQGDVLDTFK 135
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.142 0.454
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,749,916
Number of Sequences: 60738
Number of extensions: 881693
Number of successful extensions: 1873
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1855
Number of HSP's gapped (non-prelim): 6
length of query: 303
length of database: 30,389,216
effective HSP length: 106
effective length of query: 197
effective length of database: 23,950,988
effective search space: 4718344636
effective search space used: 4718344636
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)