ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIV1584 good S KOG2722 Function unknown Predicted membrane protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIV1584 545738 544440 -433
(433 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR287w [S] KOG2722 Predicted membrane protein 508 e-144
SPAC5D6.04 [S] KOG2722 Predicted membrane protein 270 2e-72
At1g76520 [S] KOG2722 Predicted membrane protein 64 6e-10
At1g71090 [S] KOG2722 Predicted membrane protein 52 2e-06
>YBR287w [S] KOG2722 Predicted membrane protein
Length = 427
Score = 508 bits (1308), Expect = e-144
Identities = 260/423 (61%), Positives = 310/423 (72%), Gaps = 7/423 (1%)
Query: 13 SFSHLSYLTFESVLQVVIISFAGFWCAYTGLLPKQGQKIVSLLNVDLFTPCLIFXXXXXX 72
SF+HL+YL FESVLQVVII+ AGFW A +GLLPKQ QKI+SLLNVDLFTPCLIF
Sbjct: 6 SFAHLAYLVFESVLQVVIIALAGFWSASSGLLPKQSQKIISLLNVDLFTPCLIFSKLAKS 65
Query: 73 XXXXXILEISIIPLFYALTTGISFLSGKLLSYLFGFDVDETNFVVANSIFGNSNSLPVSL 132
I EI+IIP+F+ LTTGISF+SGK++S + D DETNFVVANS+FGNSNSLPVSL
Sbjct: 66 LSMAKIFEIAIIPIFFGLTTGISFISGKIMSRILDLDKDETNFVVANSVFGNSNSLPVSL 125
Query: 133 TLSLAYTLPDLTWDQIPDDSRDNVASRGILYLLIFQQIGQVLRWSWGYNKLMRWSEDSVH 192
TLSLAYTLP+LTWDQIP+D+RDNVASRGILYLLIFQQIGQ+LRWSWGYNKLM+WS ++
Sbjct: 126 TLSLAYTLPNLTWDQIPNDNRDNVASRGILYLLIFQQIGQMLRWSWGYNKLMKWSGENTQ 185
Query: 193 HNHRISSQLESQATLGNDPNNVXXXXXXXXXXXXXXXXXVNLRESPYYAGTFT---KLKQ 249
H + Q+ L PN N R + + + + K+ Q
Sbjct: 186 H----MPPSQVQSLLERTPNIDNEELVNEEQEEQELLEEENNRMNSSFLSSSSIGDKIWQ 241
Query: 250 QTCFYIAKVRSFMNPPLYSMLFSVIVASIPPLQHEFFQADGFINNTLSEAIAELGSVSIP 309
++C ++R+ +NPPLYSM+F+V+VA+I PLQ E F DGFINNT +EA+ +LGSVSIP
Sbjct: 242 KSCTVFERIRANLNPPLYSMIFAVVVAAIGPLQRELFMEDGFINNTFAEAVTQLGSVSIP 301
Query: 310 LILIVLGSNLYPSGETAALTKNHKKLVFASIMGRMVLPSCFILPIIAGCVKFIKVSILDD 369
LIL+VLGSNLYPS E T +H KL+ SI+GRM+LPSCF+LPIIA VK+I VSILDD
Sbjct: 302 LILVVLGSNLYPSAEVFPKTVHHSKLLIGSIIGRMILPSCFLLPIIAIAVKYINVSILDD 361
Query: 370 PIFLVVGFILTVSPPAIQLTQITQLNEFFEAEMANVLFWGYXXXXXXXXXXXXXXXXXXX 429
PIFLVVGF+LTVSPPAIQLTQITQLNEFFEAEMA++LFWGY
Sbjct: 362 PIFLVVGFLLTVSPPAIQLTQITQLNEFFEAEMADILFWGYAVLSLPVSIIVVSGAIYVL 421
Query: 430 QWA 432
QWA
Sbjct: 422 QWA 424
>SPAC5D6.04 [S] KOG2722 Predicted membrane protein
Length = 452
Score = 270 bits (691), Expect = 2e-72
Identities = 153/410 (37%), Positives = 223/410 (54%), Gaps = 23/410 (5%)
Query: 23 ESVLQVVIISFAGFWCAYTGLLPKQGQKIVSLLNVDLFTPCLIFXXXXXXXXXXXILEIS 82
ES L+V++I+ G+ A G LP+ QK++S LNV FTPCL+F ++++S
Sbjct: 21 ESDLEVIVIALGGYVLAKKGFLPRDAQKVISSLNVYFFTPCLVFEKVGNGLNLKMLIDLS 80
Query: 83 IIPLFYALTTGISFLSGKLLSYLFGFDVDETNFVVANSIFGNSNSLPVSLTLSLAYTLPD 142
++P+FY + + S L LL+ LF + NF A F NSNSLP++L SLA T+ D
Sbjct: 81 LLPVFYVIISAASILISFLLAKLFRLTPRQRNFATACITFQNSNSLPLALVSSLATTVKD 140
Query: 143 LTWDQIPDDSRDNVASRGILYLLIFQQIGQVLRWSWGYNKLM--------------RWSE 188
L WD+IPDD+ D VASRGI+YLLIF Q+GQ LRWS+GY L+ WS
Sbjct: 141 LLWDKIPDDTPDKVASRGIMYLLIFSQLGQALRWSYGYRILLSPNQPEDPLPIGNRSWSH 200
Query: 189 DSVHHNHRISSQLESQATLGNDPNNVXX---XXXXXXXXXXXXXXXVNLRESPYYAGTFT 245
V + I + L S A + N+V V + S G F
Sbjct: 201 SDV-NEEEIQNLLASSANVDGVQNSVQANEGSTVQTDSSAISKNDNVQVETSNEEVGGFG 259
Query: 246 KLKQQTCFYIAKVRSFMNPPLYSMLFSVIVASIPPLQHEFFQADGFINNTLSEAIAELGS 305
+ +I + F +PPLYS+ ++ +A +PPLQ FF+ F+ +++ I G
Sbjct: 260 AASSKISKFIVLLLDFFSPPLYSLFIALFIAVVPPLQRFFFEEGSFVEGSITSGIRMAGQ 319
Query: 306 VSIPLILIVLGSNLYPSGETAALTK-----NHKKLVFASIMGRMVLPSCFILPIIAGCVK 360
V++P+IL+VLG++L T+ N +++ ++GRMV+ +LP +
Sbjct: 320 VAVPMILVVLGASLATDISKTEPTQEVRKNNDTRVIIVCLLGRMVVVPLALLPAFSLLSY 379
Query: 361 FIKVSILDDPIFLVVGFILTVSPPAIQLTQITQLNEFFEAEMANVLFWGY 410
F ++S +DDP+F+VV F+L SP AIQLTQI QLN FE E A VL+W Y
Sbjct: 380 FSEISTVDDPVFVVVIFLLVGSPTAIQLTQICQLNGVFERECAKVLWWSY 429
>At1g76520 [S] KOG2722 Predicted membrane protein
Length = 390
Score = 63.5 bits (153), Expect = 6e-10
Identities = 73/390 (18%), Positives = 157/390 (39%), Gaps = 37/390 (9%)
Query: 25 VLQVVIISFAGFWCAYTG--LLPKQGQKIVSLLNVDLFTPCLIFXXXXXXXXXXXILEIS 82
V+++++I+ GF+ A G LL +K ++ + +F+P LI ++++
Sbjct: 15 VVEILLITSVGFYMALDGVNLLGHDARKYLNNIVFYVFSPSLIGSRLADSVTYESLVKMW 74
Query: 83 IIPLFYALTTGISFLSGKLLSYLFGFDVDETNFVVANSIFGNSNSLPVSLTLSLAYTLPD 142
+P+ LT I L G ++ + ++ GN ++P+ + ++
Sbjct: 75 FMPVNVLLTFIIGSLLGWIVIVITKPPSHLRGLILGCCAAGNLGNMPLIIIPAVCKEKGG 134
Query: 143 LTWDQIPDDSRDNVASRGILYLLIFQQIGQVLRWSWGYNKLMRWSEDSVHHNHRISSQLE 202
P ++ G+ Y+ + +G + W++ YN LMR
Sbjct: 135 ------PFGDPESCQKYGMGYVALSMAMGSIYIWTYVYN-LMR----------------- 170
Query: 203 SQATLGNDPNNVXXXXXXXXXXXXXXXXXVNLRESPYYAGTFTKLKQQTCFYIAKV--RS 260
L N P E+ AG + K+K++ KV ++
Sbjct: 171 ---VLSNSPVETPPSVESNYDSYKVPLISSKEEENNQKAGRWEKVKRRLVSLSQKVNLKT 227
Query: 261 FMNPPLYSMLFSVIVASIPPLQHEFFQADGFINNTLSEAIAELGSVSIPLILIVLGSNLY 320
P + + ++++ I PL+ + + L +++ +G ++P + +++G NL
Sbjct: 228 IFAPSTIAAMIALVIGLITPLRKLIIGTEAPLR-VLQDSVTLVGDGAVPAMTMIIGGNLL 286
Query: 321 PSGETAALTKNHKKLVFASIMGRMVLPSCFILPIIAGCVKFIKVSILDDPIFLVVGFILT 380
++ + + + ++ R VL + I+ G K V+ +P++ V +
Sbjct: 287 KGLRSSGMKMSS---IIGVLVARYVLLPMSGVLIVRGAYKLDLVT--SEPLYQFVLLLQY 341
Query: 381 VSPPAIQLTQITQLNEFFEAEMANVLFWGY 410
PPA+ L ITQL E+E + ++ W Y
Sbjct: 342 AVPPAMNLGTITQLFGTGESECSVIMLWTY 371
>At1g71090 [S] KOG2722 Predicted membrane protein
Length = 457
Score = 51.6 bits (122), Expect = 2e-06
Identities = 42/153 (27%), Positives = 75/153 (48%), Gaps = 9/153 (5%)
Query: 258 VRSFMNPPLYSMLFSVIVASIPPLQHEFFQADGFINNTLSEAIAELGSVSIPLILIVLGS 317
V+ + PP + L ++I+ S+P L+ F D ++ +++++ +GS +P +++VLG
Sbjct: 283 VKHILQPPTIASLLAIIIGSVPQLKSVVFGYDAPLS-FITDSLNIMGSAMVPSVMLVLGG 341
Query: 318 NLY--PSGETAALTKNHKKLVFASIMGRMVLPSCFILPIIAGCVKFIKVSILDDPIFLVV 375
L P+ T L + + S+ +VLP I I+ K +S D P+F V
Sbjct: 342 MLSEGPNESTLGL----RTTIGISVARLLVLPLVGI-GIVMSADKLGLISSAD-PMFKFV 395
Query: 376 GFILTVSPPAIQLTQITQLNEFFEAEMANVLFW 408
+ +P AI L I L + E + +LFW
Sbjct: 396 LLLQYSTPSAILLGAIASLRGYAVREASALLFW 428
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.324 0.140 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,878,863
Number of Sequences: 60738
Number of extensions: 815557
Number of successful extensions: 2060
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2051
Number of HSP's gapped (non-prelim): 6
length of query: 433
length of database: 30,389,216
effective HSP length: 109
effective length of query: 324
effective length of database: 23,768,774
effective search space: 7701082776
effective search space used: 7701082776
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)